26 results about "Diapause" patented technology

The invention relates to a method for improving the diapause egg rate of silkworm germplasm resources, which comprises the following steps: during the period from pupation completion to silkworm moth eclosion, illumination period regulation of 8-hour illumination and 16-hour darkness is adopted, a light source does not contain an ultraviolet wave band, the illumination intensity is 350lx-600lx, and the light source is uniformly distributed; the temperature in the illumination period is maintained at 26-28 DEG C, the temperature in the dark period is reduced to 23-25 DEG C to form a day-night temperature difference, and the temperature change and the illumination period are switched synchronously or adjusted gradually in stages; the relative humidity in the illumination period is controlled to be 70-75%, the relative humidity in the dark period is improved to be 75-85%, and the humidity change is synchronous with illumination period switching. The method is mainly used for silkworm germplasm production by regulating and controlling the photoperiod to cooperate with temperature and humidity changes and simulating a natural autumn cooling signal to induce diapause, the storable and planned production of silkworm eggs is guaranteed, and resource waste is reduced.

The invention relates to the technical field of agricultural biology, in particular to application of a neuropeptide gene CpILP6 in relieving diapause of codling moths. After siRNA is microinjected to silence CpILP6 in a targeted manner, the diapause state of CpILP6 is changed and tends to a normal development phenotype; the research result of the invention provides a theoretical basis for defining the regulation function of the neuropeptide gene CpILP6 in the diapause induction and maintenance process of codling moth.

The invention discloses a method for regulating and controlling migratory locust embryo diapause by a BTB transcription factor LOCMI00260 and application of the method. According to the present invention, the full length of the BTB transcription factor LOCMI00260 is cloned from migratory locust, the specific double-stranded RNA interference sequence fragment dsRNA of the BTB transcription factor LOCMI00260 is developed, and the dsRNA of the BTB transcription factor LOCMI00260 is introduced into the migratory locust egg by using the microinjection method; the result shows that the migratory locust BTB transcription factor LOCMI00260 can improve the diapause rate of migratory locust. The invention provides a new means and thought for migratory locust prevention and treatment.

The invention relates to the field of economic animal feeding, and particularly discloses a technical method for promoting efficient and healthy breeding of bumblebees based on circadian rhythm, which comprises the following steps: breaking the diapause state of queen bees by using high-concentration COs, recovering the activity of the queen bees, transferring the recovered queen bees into an early-stage queen bee nesting feeding box, and providing normal pollen and liquid food; putting the queen bee breeding box into an incubator, simulating a natural photoperiod environment at the temperature of 28-29 DEG C and the humidity of 50-60%, and helping the queen bee to adapt to the circadian rhythm; at the beginning, the feeding amount of the queen bees every day is equal to the size of soybeans, and after the first batch of worker bees are hatched, the queen bees, the worker bees and honeycombs are transferred into a larger feeding box; according to the method, the biological clock of the queen bee is adjusted by controlling time service factors such as illumination, temperature, humidity and food, the survival rate of the queen bee is remarkably increased, the growth and development of the queen bee and the health state of a swarm are improved, and the technical method for promoting efficient and healthy breeding of the bumblebees based on the circadian rhythm is established.

The invention relates to the technical field of biology, in particular to a diapause and storage method for apis orientalis. Through diapause induction and long-term storage of diapause larvae of the apis orientalis, the shelf life of the apis orientalis is remarkably prolonged, the production cost is reduced, and technical guarantee is provided for annual production and large-area field application of the apis orientalis. The method comprises the following steps: carrying out diapause induction on 4-instar larvae of the apis orientalis at the temperature of 20-30 DEG C and the illumination period of 16L: 8D to obtain the apis orientalis in the diapause period; and storing the diapause-period aphanus orientalis at the temperature of 20-30 DEG C and the illumination period of 16L: 8D for 30-180 days.

The application discloses an application of a nuclear receptor target gene in preparation of a biological preparation for blocking activation of diapause larvae, and relates to the field of biotechnology and parasite prevention and control. According to two nuclear receptor targets of Haemonchus contortus HCON_00101910 (regulating activity of worms) and HCON_00023750 (regulating development of larvae), the two nuclear receptor targets are combined with host progesterone and estradiol and are activated, constitute a key signal path in the spring activation process of diapause larvae, and have no homologous genes in mammals, and the safety is excellent. By constructing specific shRNA lentivirus vectors (the silencing efficiency is all greater than or equal to 60%) targeting the two genes, single target or double target synergistic intervention is realized, and the activity-development double key links of worms in the host body can be specifically blocked. The application provides a new paradigm of preventive prevention and control with high specificity and green safety, and provides core technical support for solving the seasonal epidemic problem of blood fluke disease.

The invention discloses a corcyra cephalonica breeding method which comprises the following steps: from the day of inoculation of corcyra cephalonica eggs, the corcyra cephalonica eggs are fed with feed for five times, the feeding time points are respectively the 10th-14th day, the 17th-21st day, the 24th-28th day, the 31st-35th day and the 38th-42nd day after inoculation, the feeding amount of the third feeding is the maximum value of all the five feeding amounts, and the feeding amount of the fourth feeding is the maximum value of all the five feeding amounts. The ratio of the third feeding amount to the second feeding amount is 1.4-1.6, and the ratio of the third feeding amount to the fourth feeding amount is 1.2-1.3. According to the method provided by the invention, the rice moths grow relatively early, the moth yield and the egg laying amount are high, meanwhile, the problems of unbalanced nutrient feeding of larvae, diapause, low yield and the like in the feeding process are avoided, and large-scale production of the rice moths is facilitated.

The application discloses a silkworm cocoon preservation method for one-time autumn release of tussah silkworm in a region of bivoltine silkworm, which comprises the following processes: after tussah cocoon picking in autumn, the tussah cocoon is placed on an indoor cocoon bed, the indoor temperature is increased to 16±1 DEG C, and is maintained for 10-15 days; natural light is used for illumination for 8 hours per day; then, dark treatment is carried out, ventilation and moisture removal are performed, pupation is performed, and pupa stomach shaping is performed; then, the tussah cocoon is placed in an environment with a temperature of 15±2 DEG C and a relative humidity of 30%-50%, diapause is performed for 60-90 days, illumination is performed for 8 hours per day, dark treatment is performed for 16 hours per day, and the illumination intensity is 150 lux; then, cocoon preservation, cocoon warming, eclosion adjustment, moth catching, moth airing, mating, egg production, mirror inspection, egg preservation, egg surface disinfection, mountain climbing and ant catching are performed; and the tussah silkworm is released by using a three-moving method and is evenly distributed, and tussah cocoon picking is performed. The application breaks the limitation of tussah silkworm in a bivoltine region, the tussah silkworm is not released in spring in a bivoltine region, and the tussah silkworm is released only once in autumn every year, so that the influence of high temperature, dry weather in spring, tussah silkworm diseases and insect pests and drug damage on the tussah silkworm is reduced, the annual yield of tussah cocoon is increased by more than 30%, the total cocoon weight is high, and the cocoon quality release result is improved.

The invention belongs to the field of plant protection, and particularly relates to a diapause control gene AjFoxO of apis cerana and application of the diapause control gene AjFoxO. According to the application disclosed by the invention, the key gene AjFoxO for diapause control of the small apis belly bees is found through screening, and the influence of the AjFoxO gene on the diapause behavior of the small apis belly bees is verified through an addition test of RNAi (Ribonucleic Acid Interfere) and exogenous juvenile hormone. The method can be used for diapause control of the small apis belly bees, so that the shelf life of parasitic wasp products is controlled, and the optimal economic benefit is achieved. And the method can also be used for breeding and screening the variety of the small apis belly so as to obtain the variety with long diapause period and serve for biological prevention and control of agriculture and forestry.

The invention discloses a method for hatching diapause silkworm eggs at any time based on plasma treatment. The method solves the problems that an existing diapause silkworm egg hatching technology depends on hydrochloric acid treatment, operation is complex, pollution is large, and hatching operation at any time is tedious. According to the technical scheme, the method is characterized in that different plasma treatment parameters and refrigeration time combinations are selected according to the egg ages of silkworm eggs; the diapause eggs can be directly treated for 5 minutes by 10 kV / cm after postpartum for 3 days or treated for 1 minute by 12 kV / cm after being refrigerated for 30 days; 4-6 days after delivery, the shallow diapause eggs need to be refrigerated for 30 days and then treated for 1 minute with 15kV / cm; after 7 days after delivery, the eggs need to be refrigerated for 35 days, and then are treated for 1 minute by 15kV / cm; after 10 days after delivery, deep diapause eggs need to be refrigerated for 50 days and then treated for 1 minute with 12 kV / cm; the refrigeration condition is 5 DEG C and the humidity is 75-85%. According to the method, diapause can be relieved simply, conveniently, efficiently and environmentally, controllable incubation of silkworm eggs at any time is achieved, and the annual continuous production requirement of the sericulture is met.

The invention belongs to the technical field of biological prevention and control of agricultural pests, and discloses dsRNA of a migratory locust retinoic acid X receptor and application of the dsRNA. The dsRNA of the migratory locust retinoic acid X receptor is specifically dsrxr, and the dsrxr interferes with or inhibits the expression of the migratory locust retinoic acid X receptor gene rxr. The designed and synthesized dsrxr can relieve migratory locust egg diapause and reduce the migratory locust egg diapause rate, and after migratory locust is injected with a dsrxr solution, the migratory locust egg diapause rate is remarkably reduced. After the dsrxr is used, diapause of migratory locust eggs is relieved in the overwintering period, hatched larvae cannot survive in cold climate, and the aim of preventing and controlling migratory locust in generation intervals is achieved.

To develop and provide a technique for making transgenic silkworms of commercial lines in a short time and with little effort.SOLUTION: A method for making transgenic silkworms includes a step for introducing an intended nucleic acid into an egg, followed by a step for breaking the dormancy of the dormant egg by non-thermal equilibrium plasma treatment.

The invention discloses a method for accurately regulating and controlling the diapause of braconidae girdle under a single condition. The method comprises the following steps: selecting Asiatic corn borers in which braconidae girdle is parasitized in a diapause sensitive stage as an object for diapause induction; regulating and controlling the temperature to induce the bees to enter a diapause state; removing the non-parasitized ostrinia furnacalis according to the morphological difference; diapause is maintained or relieved under the original induction condition, and diapause is relieved under the conventional feeding condition. According to the method, the regulation and control opportunity is accurately mastered, and the temperature of a single condition is regulated and controlled, so that the cotesia girdle enters diapause without depending on the diapause state of the host ostrinia furnacalis, the limitation of inducing the diapause of the host is overcome, and convenient and efficient diapause regulation and control are realized. The diapause maintenance process is simple and convenient, the storage period is prolonged, after the release, the quality can be ensured, batch-by-batch or centralized eclosion adult bees can be flexibly obtained, and large-scale production and efficient release and utilization are facilitated.

The invention belongs to the technical field of biological prevention and control of agricultural pests, and particularly relates to a control method for inducing diapause of noctuid black egg bees. The method comprises the following steps: a, establishing a prodenia litura population and collecting new spawning blocks; b, continuously breeding the noctuid black-egg bees by taking the fresh prodenia litura eggs in the step a as hosts, and collecting female noctuid black-egg bees which are just subjected to eclosion; c, the prodenia litura eggs in the step a are inoculated with the noctuid black egg bee female bees in the step b for parasitism; and d, transferring to a diapause induction environment for treatment after parasitism. Under the conditions that the temperature is 8 DEG C and the photoperiod is 0L: 24D, or under the conditions that the temperature is 12 DEG C and the photoperiod is 8L: 16D, the diapause rate after 35-day induction treatment is relatively high, and the diapause rate reaches 97.5% or above. According to the method disclosed by the invention, the diapause rate of the black egg noctuid is remarkably improved through coordinated regulation and control of the temperature, the photoperiod and the induction time, and a key technical support is provided for long-term storage and large-scale application of the black egg noctuid.

The invention discloses a method for intervening growth of echinococcus multilocularis based on diapause pheromone Ascr # 18 and application, and belongs to the technical field of prevention and control of zoonosis parasitic diseases. According to the invention, the Ascr # 18 is identified in the echinococcus multilocularis through liquid chromatography-mass spectrometry (LC-MS) for the first time, and it is verified that the Ascr # 18 can be combined with a downstream G-protein coupled receptor (GPCR) in a high-affinity manner, and a signal channel is activated. After the Ascr # 18 is treated, the expression level of development key genes daf-9 and daf-12 in the protoscolex of the echinococcus multilocularis can be remarkably reduced, and the movement activity of the development key genes daf-9 and daf-12 is inhibited, so that the development process and infection ability of parasites are blocked. The invention provides a novel biological prevention and control strategy which does not depend on traditional chemical anthelmintic drugs, is high in targeting, high in safety, environment-friendly and controllable, is suitable for comprehensive prevention and control of alveolar echinococcosis, and can also provide a theoretical basis and an application reference for prevention and control of other tapeworm and larva parasitic diseases.

The invention belongs to the technical field of animal molecular marker screening and application. The artificial climate box for diapause maintenance of the procambarus clarkii comprises a cabinet body, a culture system, a water circulation system, a temperature control system, an oxygenation system and an electric power system. The cabinet body realizes efficient heat insulation. The breeding system is divided into a plurality of independent storage bins through transverse and vertical stainless steel filter screens, and brushes are arranged to increase the attachment surface of shrimp seeds. The water circulation system is combined with the water filtering device and the inner and outer double-pipe type dirt collecting device to achieve multi-stage filtering and removing. The power system is provided with a power-off alarm and a standby power supply to guarantee continuous operation of the system. The diapause state of the procambarus clarkii larvae can be effectively induced and prolonged by cooperatively regulating and controlling water cleanliness, low-temperature stability and dissolved oxygen sufficiency, intensive long-term storage of the procambarus clarkii larvae is achieved, seasonal supply limitation is broken through, and finally the industrial target that the procambarus clarkii larvae exist in four seasons and the procambarus clarkii exists in four seasons is achieved.

The invention provides a diapause maintenance and storage method for procambarus clarkia, which comprises the following steps: selecting female shrimps with ovary development to IV stage or V stage in an aquaculture pond, collecting and transferring the majority of the female shrimps into an aquarium in an artificial climate box after the majority of the female shrimps become lobster shrimps, and enabling the adult shrimps and the lobster shrimps to enter a diapause state at the same time by regulating and controlling the photoperiod and the temperature; feeding commodity expanded feed and cleaning residual feed and excrement in time. By means of the storage method, precise control over diapause maintenance of the procambarus clarkii is achieved, large-scale full-year fry supply and full-year cultivation of the procambarus clarkii can be achieved, backcross breeding material construction of offspring and parents can be achieved, and efficient intensive germplasm bank construction for conservation of important germplasm resources can be achieved; according to the method, the healthy, efficient and sustainable development level of the procambarus clarkii industry can be effectively improved.

This invention discloses a method for rapidly removing diapause from dormant brine shrimp eggs cultured indoors and improving the hatching rate, relating to the field of brine shrimp culture using natural aquatic feed. The method includes the following steps: A. Collection and preservation of dormant brine shrimp eggs; B. Pretreatment of dormant brine shrimp eggs; C. Incubation under constant light. This method can shorten the diapause removal time for indoor-cultured brine shrimp eggs to less than 20 days and increase the hatching rate to 92.5%.

The invention belongs to the technical field of agricultural biology, and particularly provides a miRNA combination for improving the diapause rate of migratory locust embryos and application of the miRNA combination. MiRNA in the miRNA combination is miR-92a and miR-92b, the nucleotide sequence of the miR-92a is as shown in SEQ ID NO: 1, and the nucleotide sequence of the miR-92b is as shown in SEQ ID NO: 2. The miR-92a agonist and the miR-92b agonist are mixed and injected into migratory locust ovaries in a long photoperiod, so that the diapause rate of migratory locust embryos is effectively increased. According to the method, the migratory locust population number can be fundamentally reduced, migratory locust disaster risk is reduced, and a new means and thought are provided for migratory locust prevention and treatment.

The utility model discloses an electrical stimulation device capable of uniformly relieving diapause of silkworm eggs for silkworm frame seed production. The electrical stimulation device comprises a high-voltage static generation and output end; the silkworm egg processing structure comprises an insulating support plate, a metal pole plate, an insulating support frame and a discharge structure; the metal pole plate is connected above the insulating support plate; the insulating support frame comprises two vertical support rods and an insulating cross rod vertically connected between the vertical support rods, and the two vertical support rods are vertically connected above the insulating support plate; the insulating cross rod is connected to the upper end of the vertical supporting rod; the discharge structure comprises an upper end, a middle section and a lower end, the upper end is connected to the high-voltage electrostatic output end, the top of the middle section is connected to the upper end, and the lower end is connected to the bottom of the middle section; wherein the metal pole plate is positioned between the two vertical support rods; the upper end, the middle section and the lower end are electrically connected, and the lower end is composed of a plurality of thin wires which are arranged in a conical shape. The device is adjusted according to the electric field tolerance degree of different silkworm varieties to meet the electric stimulation conditions required by silkworm eggs.

The present invention discloses a method for recovering honeycomb cells and monitoring offspring development of alfalfa leafcutter bees, comprising the following steps: 1. placing honeycombs and hanging honeycomb panels; 2. inserting paper straws into the honeycomb panels; 3. releasing adult bees; 4. recovering paper straws; 5. cultivating larvae; 6. cultivating prepupae; and 7. performing data statistics and analysis. The method for recovering honeycomb cells and monitoring offspring development provided by the present invention can promptly and quickly recover newly constructed honeycombs by female bees, monitor the development of offspring in real time, and obtain information such as the diapause rate, offspring mortality rate, natural enemy damage rate, empty nest rate, and pollen ball percentage of offspring recovered within a selected time period. This provides a simple and rapid monitoring technology for timely and accurate understanding of the causes of bee losses during the propagation and application of alfalfa leafcutter bees. The method provided by the present invention can guide those skilled in the art to take timely measures to reduce offspring losses, and provide more efficient and high-quality pollination services for alfalfa seed production by protecting and utilizing alfalfa leafcutter bees.

The present application belongs to the technical field of aquaculture, and relates to a method for recovering and growing of Procambarus clarkia diapause fry, which comprises two stages of diapause recovery and growing: first, the diapause fry is placed in recovery liquid A, and slowly warmed to room temperature at a rate of 2 DEG C per day to realize recovery of the vitality of the fry; then, the recovered fry is transferred to a growing system, and through management measures such as regulation of water quality (pH 7.5-8.6, salinity 0.10-0.50 ‰, water temperature 15-30 DEG C), hierarchical feeding (special feed in the early stage, and shrimp feed in the later stage), regular water change and probiotic supplement, the fry is cultivated to a size of greater than or equal to 1 g. The present application significantly improves the recovery rate (greater than or equal to 95%) and the survival rate (greater than or equal to 88%) of the diapause fry, shortens the cultivation period, promotes the cultivation of large-scale and high-uniformity fry, and provides stable fry source for the out-of-season cultivation of Procambarus clarkia.

This invention discloses a method and system for predicting the diapause rate of bipolar silkworms based on the fusion of multi-source environmental factors and molecular markers. The method first collects multi-source environmental data during the critical growth and development period of the female silkworm generation, including temperature, light duration, and feed amount; simultaneously, it acquires molecular marker data closely related to diapause induction. Through a unique feature engineering approach, the environmental data is transformed into biologically meaningful feature vectors, which are then deeply fused with the molecular marker data to construct an input feature set. This set is then used to train a gradient boosting decision tree model, resulting in a silkworm diapause rate prediction model. The prediction model is then used to predict new diapause rates in silkworms. This invention overcomes the limitations of traditional diapause identification methods, which are often delayed and destructive, achieving a forward-looking and quantitative accurate prediction of population diapause rates, providing a core tool for the intelligent regulation of silkworm production.

The application discloses a method for interfering with the growth of multilocular echinococcosis based on diapause pheromone Ascr#18 and application thereof, and belongs to the technical field of prevention and control of parasitic zoonosis. daf-9 With daf-12 The application discloses a method for interfering with the growth of multilocular echinococcosis based on diapause pheromone Ascr#18 and application thereof, and belongs to the technical field of prevention and control of parasitic zoonosis. The application discloses a method for interfering with the growth of multilocular echinococcosis based on diapause pheromone Ascr#18 and application thereof, and belongs to the technical field of prevention and control of parasitic zoonosis. The application provides a novel biological prevention and control strategy which is independent of traditional chemical deworming drugs, has high targeting, high safety, is environment-friendly and controllable, and is suitable for comprehensive prevention and control of alveolar echinococcosis, and can also provide a theoretical basis and application reference for prevention and control of other cestode larva parasitic diseases.

The present invention discloses a cultivation method for improving the molting rate and survival rate of diapause first-instar Chinese horseshoe crabs. The diapause first-instar Chinese horseshoe crabs refer to individuals that are hatched into first-instar juveniles in September of the current year and have not molted into second-instar horseshoe crabs until April of the following year. The method comprises the following steps: selecting diapause first-instar Chinese horseshoe crabs and placing them in seawater containing 30-90 mg / L of vitamin C for cultivation. The present invention can not only effectively improve the molting rate of diapause first-instar juvenile horseshoe crabs but also improve the survival rate of the juvenile horseshoe crabs, thereby obtaining second-instar horseshoe crab seedlings with good quality.