140 results about "Diapause" patented technology

The present invention discloses an artificial breeding and generation method for thyestillagebleri, and an artificial feedstuff for the thyestillagebleri larvae. The method comprises the following steps: placing a single mature thyestillagebleri larva in a test tube at a temperature of 20-30 DEG C and relative humidity of 60-80%; after pupating and emerging, carrying out pairing for the imago, then placing the paired imagoes in a container provided with piemarker stems to carry out copulation and oviposition; carrying out disinfection for eggs; placing the hatched larvae in a container filled with the artificial feedstuff to breed until the larvae mature, wherein the artificial feedstuff in the container is replaced for a plurality of times. A formula of the artificial feedstuff comprises, by weight, 5-20% of corn flour, 5-20% of soybean powder, 3-10% of maggot powder, 3-10% of sugar, 3-7% of agar, 0.1-1% of Vc, 0.1-1% of a preservative and 50-70% of water. According to the present invention, the thyestillagebleri is bred through the method provided by the present invention, the diapause of the larvae is not generated, the developmental duration is substantially shortened; the feedstuff does not contain the host component; the main components are easy to obtain; the production method has characteristics of simple process, low cost and no seasonal restriction, and is applicable for large-scale indoor breeding.

The invention relates to a method for a silkworm diapause variety low temperature egg incubation transgene. Silkworm diapause variety next generation graine voltinism is changed in the special egg incubation condition, to ensure that present generation silkworm diapause variety moths obtained under the special egg incubation condition mate, to give birth to non-diapause next generation graine, finally referring to the microinjection transgene method of the non-diapause silkworm eggs embryo, the gene transferring is performed, therefore the obstruction of the silkworm diapause variety transgene technology is expertly broken, and a transgene silkworm is effectively manufactured. The method directly adopts the universal use utility silkworm diapause variety as original material in production, can avoid the long and troublesome breeding process, and directly utilizes the transgene technology to create the good new special silkworm variety with various characteristics, the breeding cycle can be greatly shortened, and massive manpower and physical resources are saved.

The invention relates to a silkworm diapause variety early pickling transgene method. The hydrochloric dipping treatment is performed to a silkworm diapause ovum, by utilizing a certain time before silkworm egg embryo is developed to a germ band formed, namely, before 2 hours and a half after egg casting, then the embryo microinjection is performed to the graine of diapause treatment canceling, the G0 generation silkworm obtained from the incubation breeding bred to eclosion, the G1 generation silkworms are produced through the idiogamy or the backcross of the silkworms, and labeled and sieved through fluorescence, and therefore the transgene silkworms are effectively manufactured. The method directly adopts the universal use utility silkworm diapause variety as original material in production, can avoid the long and troublesome breeding process, and directly utilizes the transgene technology to create the good new special silkworm variety with various characteristics, the breeding cycle can be greatly shortened, and massive manpower and physical resources are saved.

The invention provides a method for making transgenic silkworms of a silkworm diapause variety. The method comprises the steps of utilizing the relationship between the environmental conditions of a parental generation and the diapause property of filial-generation silkworm eggs and utilizing a generation before transgenic operation to control the environmental conditions of the diapause silkworm variety so as to change the diapause property of a present generation of transgenic operation, performing silkworm-egg embryo microinjection on non-diapause silkworm eggs, utilizing fluorescent protein labeling to screen and making the transgenic silkworms. The method has the advantages of ingeniously breaking through the obstacles of transgenic technology for the silkworm diapause variety and effectively overcoming the defects of the prior transgenic method, and can lay a solid technical foundation for the large-scale application of silkworm transgenic technology in functional gene research, bioreactor development and other practical research.

A colorful silkworm cocoon creation method by pigment protein is disclosed. A pigment protein determining color of coral reed and related marine creature is fusion expressed in bombyx mori silk fibroin by genetic modification of silkworm and especially genetic modification for diapause varieties, so that novel colored silkworm cocoon is created. Exogenous pigment protein is fusion expressed in silk fibroin, and exists stably and uniformly in silk fibroin molecular, so pigment protein will not be lost in silk reeling procedure, and color variety of pigment protein is abundant. The method used by the invention solves two problems of little variety in color of silkworm cocoon and instability in pigment binding, simplifies spinning process of silk fabrics, eliminates harm of dyeing process to silk fiber quality and human health.

The invention provides a constant-temperature inducing maize-borer oophagous trichogrammae diapause storage method. The collected maize-borer oophagous trichogrammaes which have developed to the ovum telophase from the middle larva stage adopt constant-temperature inducing maize-borer oophagous trichogrammae diapause, and are stored under a specific temperature and humidity, and the development of the maize-borer oophagous trichogrammae is restored by changing the prior temperature to use. The method is applicable to oophagous trichogrammae breeding by using small gelechiid ovum; the technology matches large-scale oophagous trichogrammae production; the method is applicable to long-term production and storage of maize-borer oophagous trichogrammaes; thereby, sufficient maize-borer oophagous trichogrammaes are prepared for pest control, and the method can also be used to store breeding maize-borer oophagous trichogrammaes.

The invention discloses a method for regulating and controlling agryponflexorius, which adopts following steps: 1), agryponflexorius to be subjected to diapause induction is placed under the condition with the temperature of 17 plus / minus 1 DEG C and a photoperiod from (L8 : D16) to (L12 : D12) for induction, and is enabled to enter a diapause state; 2), the agryponflexorius entering the diapause state in the step 1 is stored under a dark condition with the temperature of 4 DEG C; and 3), after being ended in storage in step 2, the agryponflexorius resumes growth under a condition with temperature of 23 DEG C and a photoperiod from L14 to D10 to L16 to D8. The method has the advantages that the diapause induction rate is 100%, the storage period after diapause is 120 days, and after 20 days from diapause removal, adult agryponflexorius can be hatched. According to the invention, the agryponflexorius can be propagated in large, the shelf life of products and time in keeping prevention and control functions in fields can be prolonged, the prevention and treatment efficiency of insects is improved, and the method has important significance in knowing on developmental mechanism of natural enemy insects and exploring adaptive mechanism of insects to environment and evolutionary approaches of insects.

The present invention relates to a breeding technique of wild bumblebee, which is mainly suitable for artificial feeding and breeding of wild bumblebee and belongs to the technical field of wild insect breeding. The breeding technique of bumblebee is composed of the following steps: capturing a queen bee of the wild bumblebee, setting a simulation environment, inducing the queen bee to lay eggs, performing low-temperature treatment to the bee colony, carefully choosing queen bees of the bee colony, causing virgin queen bees to copulate, refrigerating the queen bees for breeding, raising the temperature for arousing the queen bees, etc. The breeding technique of wild bumblebee is characterized by the copulation of the virgin queen bees. The newborn queen bees which are mature in sexual development and drones are caused to fly for 4 hours in a small-size shelter which has enough light and is provided with flowering fruits or vegetables in a temperature of 25-30 DEG C and a relative humidity of 60-65%. The queen bees for breeding are refrigerated. The queen bees are stored in a refrigerating chamber of a refrigerator with a relative humidity of 60-75% and a temperature of 0-5 DEG C for causing the queen bees to enter a dormancy diapause state along with the simulation of natural temperature and humidity. The breeding technique of the wild bumblebee has the following advantages: high success rate of bumblebee queens, high egg laying rate, high herding rate of bumblebee, etc.

The invention discloses a method for regulating diapause of aphidius gifuensis ashmead. The method provided by the invention comprises following step 1) or 2), wherein the step 1) comprises the following steps of: A, treating aphidius gifuensis ashmead larva at 5-15 DEG C and with a thermoperiod being C18: T6 and a photoperiod being L8: D16 so as to obtain the aphidius gifuensis ashmead in a diapause state; B, carrying out diapause termination on the aphidius gifuensis ashmead in the diapause state to obtain hatched aphidius gifuensis ashmead; and the step 2) comprises the following steps of: C, carrying out diapause treatment on the aphidius gifuensis ashmead larva at a low temperature and with a photoperiod being L8: D16 to obtain the aphidius gifuensis ashmead in the diapause state, wherein the low temperature is no more than 12 DEG C; and D, carrying out diapause termination on the aphidius gifuensis ashmead in the diapause state to obtain the hatched aphidius gifuensis ashmead. The experimental results of the invention show that the method provided by the invention can realize accurate regulation on the development progress of the aphidius gifuensis ashmead.

The invention relates to a flounder triploid fry batch producing method, which comprises a triploid fry induction method, a method for identifying the triploid produced by the method, and a method for evaluating triploid adult fish growing performance and gonadal development. By the method, a triploid flounder fry batch producing method by adopting low-temperature shock is established, and proper starting time and proper low-temperature treatment time of triploid induction are determined; a method for identifying the triploid fry is established; and the method for evaluating triploid fish growing and gonadal development situation is established. Through the triploid fry induction method, the triploid fry is inducted and generated efficiently in batches, the induction efficiency of the triploid fry is up to 100 percent, the growing speed of the induced triploid fish is 20 percent higher than that of the diploid fish, and the gonad is in diapauses. The method is easy to operate, practical, safe and reliable, has strong practicability, and opens a novel technical method for sex control of paralichthys olivaceus and sole and multiploid breeding.

The invention discloses a diapause induction and cancellation method of natural enemy insect Coccinella septempunctata. The method comprises the steps of inducing adult of Coccinella septempunctata under condition of 18 DEG C and lighting period of L10:D14 to enable Coccinella septempunctata to enter diapause state; and transferring the Coccinella septempunctata in diapause state to a condition of 24 DEG C and lighting period of L16:D8 to recover development. The method has diapause induction rate close to 100% and diapause canceled in 7.52-8.62 days. The method is of great significance for large-scale propagating Coccinella septempunctata, prolonging shelf period and field prevention / control action time, grasping natural enemy insect development characteristic and occurrence dynamics, improving pest prevention / control efficiency, deepening understanding about natural enemy insect development mechanism, and investigating environment adaptability mechanism and evolution route of insect.

The invention discloses a coccinella septempunctata diapause control method. The method comprise the following steps: (1) inducing a coccinella septempunctata adut under the conditions that the temperature is 18 DEG C and photoperiod is L10: D14, so that the coccinella septempunctata enters into a diapause state: (2) storing the coccinella septempunctata in the diapause state under the conditions that the temperature is 18 DEG C and the photoperiod is L10: D14 (or darker); and (3) after finishing the storage, carrying out recovery development on the coccinella septempunctata under the conditions that the temperature is 24 DEG C and the photoperiod is L16: D8. By using the coccinella septempunctata diapause control method provided by the invention, the diapause induction rate is near 100%, the storage term after the diapause can reach 114-183 days, and the diapause can be released for 7.52-8.62 days. The method has important effects of multiplying the coccinella septempunctata in large scale, prolonging the product shelf life, having field prevention and control functions, mastering the growing characteristics and develoment of the natural enemy insect, improving the pest control efficiency, enhancing the knowledge of the developmental mechanism of the natural enemy insect, exploring the adaptive mechanism and evolution of the insect to the environment and the like.

The invention discloses a migratory locust serine protease inhibitor 7 and a coding gene and application thereof. By means of a gene cloning technology, a migratory locust LmSerpin7 gene is cloned from migratory locusts, dsRNA used for interfering a migratory locust LmSerpin7 gene is synthesized, and dsRNA is guided into the migratory locusts through an injection method to conduct RNAi on the migratory locust LmSerpin7 gene. The result shows that the gene can regulate migratory locust egg diapause, and a theoretical basis is provided for further deeply understanding the migratory locust diapause mechanism and providing new biological pesticide target sites.

The invention belongs to the technical field of resource insect exploitation and utilization, and particularly relates to a method for timely obtaining adult sericinus montelus gray by manually regulating pupa diapause. The method is characterized by comprising the following steps of: when initially hatching the larvae, feeding sericinus montelus gray larvae under the periodic conditions of the normal temperature of 15 to 25 DEG C and short light / dark cycle of 8 / 16 to 12 / 12 hours to induce diapause of sericinus montelus gray pupae so as to obtain sericinus montelus gray diapause pupae; preserving the sericinus montelus gray diapause pupae for 15 to 30 days under the conditions of the temperature of 0 to 10 DEG C and complete darkness to promote the diapause development of the sericinus montelus gray diapause pupae; and transferring the sericinus montelus gray diapause pupae after low-temperature preservation to the conditions of the temperature of 25 to 28 DEG C and the light / dark cycle of 14 / 10 to 16 / 8 hours to terminate diapause. The method realizes centralized eclosion of the sericinus montelus gray diapause pupae within 4 to 5 days, and provides butterfly sources of timely butterfly flying for scenic spot building, celebration activities and the like.

The invention relates to a method for instant relieving diapauses by heat shock and delaying hatching by cold storage for bombyx mori. The method comprises the following steps: S1, incubating silkwormeggs subjected to acid leaching until the silkworm eggs are hatched, and carrying out breeding until cocooning is achieved; S2, collecting male and female silkworm moths which come out at the same time, mating the male and female silkworm moths for 4-5 hours, then separating copulating moth, and putting the female moths on silkworm egg paper for laying eggs; S3, after eggs are laid, protecting silkworm egg paper containing the silkworm eggs into a incubator, carrying out heat shock with hot water for 5 seconds after a preset time, carrying out airing, dividing the silkworm eggs into three parts; and S4, incubating the first part of silkworm eggs, collecting newly hatched silkworms and carrying out breeding; incubating the second part of silkworm eggs, placing the silkworm eggs into a middle storage for 2 hours when the silkworm eggs turn green, wrapping the silkworm eggs with film, placing the silkworm eggs into a cold storage, taking out the silkworm eggs of a preset amount every 3 days, placing the taken silkworm eggs into the middle storage for 2 hours, then placing the silkworm eggs in an environment of 25 DEG C, collecting newly hatched silkworms and carrying out breeding; and placing the third silkworm eggs into the middle storage for 6 hours, then wrapping the silkworm eggs with film in the cold storage, after the silkworm is stored for 30 days, taking a part of the silkworm eggs every 5 days, placing the silkworm eggs into the middle storage for 24 hours, then collecting newly hatched silkworms and carrying out breeding.

The invention relates to a method for creating a novel green cocoon practical variety of silkworms by using GFP protein. The method comprises the step of fusing large amount of the GFP protein in chromoproteins expressing and deciding colors of related marine organisms such as coral reefs and so on in silkworm fibroin by using a transgenic technology for the silkworms, in particular a transgenic technology for a diapause variety, so as to quicken cultivating the novel green cocoon practical variety of the silkworms. The method has the advantages that the method not only quickens practical process of transgenic silkworms greatly, but also produces fiber fabrics capable of being directly applied to weaving silk without being printed and dyed, simplifies spinning technology greatly, saves production cost and is favorable for environmental protection.

The method of incubating brine shrimp cyst to produce free swimming nauplius brine shrimp larva is to setting brine shrimp cyst and the additive in the weight ratio of 100 to 0.5-4 simultaneously into incubating culture medium. The additive is organic benzene containing one or several o-hydroxy radicals, such as pyrogallic acid, methyl pyrogallate, tannic acid, tanning extract, ea polyphenol, etc. The additive can damage the diapause mechanism of brine shrimp cyst to raise the brine shrimp cyst incubating within 24 hr to 90 %, and has flocculating effect to separate nauplius brine shrimp larva easily from un-incubated brine shrimp cyst. The present invention expands the application range of incubating culture medium and is especially suitable for incubating treatment of brine shrimp cyst the inland brine lake produces.

The invention provides a method for regulating and controlling diapause of microplitis syntretomorpha. The method includes the steps of diapause induction, diapause storage and diapause relieving. In the step of diapause induction, prodenia litura where microplitis syntretomorpha is parasitized for 1 day to 3 days is independently subpackaged in flat-bottom test tubes, the test tubes are placed under the condition with the temperature of 18+ / -1 DEG C, the relative humidity of 70+ / -10% and the photoperiod of L10:D14 for induction, and microplitis cocoons in the diapause state are obtained after host larvae come out of the cocoons. In the diapause storage step, wet fine sand is laid on the diapause cocoons after the host larvae are removed, the diapause cocoons are transferred to the dark environment with the temperature of 6 DEG C to be stored. In the diapause relieving step, the diapause cocoons are placed under the condition with the temperature of 27+ / -2 DEG C, the humidity of 70+ / -10% and the photoperiod of L14:D10, and eclosion microplitis syntretomorpha is obtained in about 5 days. The diapause rate of microplitis syntretomorpha induced through the method can reach more than 98%, the diapause cocoons are stored for 3 months to 5 months under the condition of 6 DEG C, and the eclosion microplitis syntretomorpha does not degenerate in the aspects of the egg laying amount, the life and other biological indicators. By means of the method, the retention cycle of microplitis syntretomorpha can be effectively prolonged by 4 times to 5 times, and the reference basis is provided for regulating and controlling development of microplitis syntretomorpha.

The invention relates to the field of insect bioengineering, in particular to a method for transgene of eggs of bivoltine silkworm varieties. In the method, early activated eggs are obtained by utilizing combination of protection temperature and light system in a parent generation silkworm egg hatching period, and artificial hatching treatment on the silkworm eggs before offspring fertilization; and then the early activated eggs are subjected to micro-injection and screening so as to obtain transgenic silkworm eggs. The method can solve the problems that the artificial hatching treatment after the diapaused eggs of the bivoltine silkworm varieties are subjected to transgenic injection causes damage to the silkworm eggs and the silkworm eggs cannot be hatched, also can solve the problem of low hatching rate due to transgenic injection after the early hydrochloric acid hatching treatment on the diapaused eggs of the bivoltine silkworm varieties, and also solves the problems that embryo robustness is poor and larva survival rate is low for offspring non-diapause eggs of the bivoltine silkworm varieties obtained by the hydrochloric acid hatching treatment on the diapaused eggs of the bivoltine silkworm varieties and low-temperature and dark hatching conditions for the parent generation silkworm eggs.

The invention discloses a method for increasing the hatching rate of diapause artemia eggs in a weak light environment. The diapause artemia eggs are hatched in a hatching culture medium, and the artemia eggs are contacted with a mixture of tea polyphenol and vitamin C sodium; and the concentration ratio of the vitamin C sodium and the tea polyphenol which are introduced into the hatching culture medium is (1:4)-(1:5). The invention aims to provide the method for increasing the hatching rate of the diapause artemia eggs in the weak light environment; and with the adoption of the method, the hatching rate is increased, the stability of the hatching rate is guaranteed, and the stable hatching rate of a large hatching place is guaranteed.

The invention discloses a diapause induction method of harmonia dimidiate. The diapause induction method comprises the steps of 1) determining the diapause control sensitive stage of the harmonia dimidiate as adjusts just after eclosion and selecting the harmonia dimidiate at the stage as the diapause induction objects, and 2) putting the harmonia dimidiate selected in the step 1) in a diapause induction environment and enabling the harmonia dimidiate to enter the diapause state by regulating and controlling the combination of illumination time each day, temperature and total induction time, wherein the illumination time each day is photoperiod (8-10)L: (16-14) D, the temperature is in the range of 13-15, and the continuous induction time is greater than or equal to 192 hours. The diapause induction method of the harmonia dimidiate is capable of realizing accurate control on the excellent natural predatory enemy insect harmonia dimidiate by controlling the environmental factors in combination necessary treatment measures; the diapause rate can be about 95%; technical guarantee is provided for annual large-scale production of the harmonia dimidiate.

The invention relates to antheraeae pemyi male pupae wine and a preparation method thereof, and belongs to the technical field of preparation of health care wine. The preparation method comprises the following steps: preparation of antheraeae pemyi male pupa in the late development stage; preparation of an extract liquid of the antheraeae pemyi male pupa in the late development stage; preparation of a traditional Chinese medicinal extract liquid; preparation of the antheraeae pemyi male pupae wine, wherein the preparation of the antheraeae pemyi male pupa in the late development stage comprises the following steps: after the termination of diapauses, heating antheraeae pemyi male pupa to 18-22 DEG C at the speed of 3-6 DEG C / d at the relative humidity 50-70% or below; keeping the constant temperature of 18-22 DEG C till the effective accumulative temperature reaches 180-200 DEG C, so as to obtain the antheraeae pemyi male pupa in the late development stage. The preparation method has the benefit that the antheraeae pemyi male pupa in the late development stage is adopted in the antheraeae pemyi male pupae wine, so that all the nutrition and active components of the male pupa are retained in the antheraeae pemyi male pupae wine.

The invention discloses special culturing salt for increasing the hatchability of brine shrimp eggs. The incubation of the brine shrimp eggs is performed in an incubation culture medium, different substances are added to the incubation culture medium and are mixed to prepare special salt in the solid powder manner according to the following corresponding percentage by weight: 85-90% of sodium chloride, 3-5% of magnesium chloride, 2-4% of magnesium sulphate, 2-3% of calcium chloride, 2-3% of potassium chloride, 1-2% of sodium bicarbonate, 0.2-0.8% of sodium carbonate, and 0.1-0.15% of calcium peroxide. The concentration of the incubation culture medium is 28-35 g / L, and the density of the brine shrimp eggs in the incubation culture medium is less than or equal to 3 g of dried brine shrimp eggs per liter of the incubation culture medium. The method not only guarantees the salinity desired by the incubation of the brine shrimp eggs, but also does not cause a side effect on the incubation of the brine shrimp eggs; most important of all, varied component salt substances and an appropriate amount of the calcium peroxide can destroy the diapause eggs in the brine shrimp eggs, and the brine shrimp eggs are promoted to incubate brine shrimp nauplius which can freely go, so that the hatchability of the brine shrimp eggs reaches 80% or above.

The invention discloses a method for adjusting the diapause of Exorista civilis Rond. The method comprises the following steps that (1) the Exorista civilis Rond with the diapause to be induced is placed in the environment with the conditions that the temperature is 17+ / -1DEG C, and the photoperiod ranges from L8:D16 to L12:D12, and induction is carried out on the Exorista civilis Rond so that the Exorista civilis Rond can be in a diapause state; (2) the Exorista civilis Rond which is obtained in the step (1), and is in the diapause state is stored in the dark with the temperature of 4DEG C; (3) after storage in the step (2) is accomplished, the Exorista civilis Rond is placed in the environment with the conditions that the temperature is 23+ / -1DEG C, and the photoperiod ranges from L14:D10 to L16:D8, and development is recovered. According to the method for adjusting the diapause of the Exorista civilis Rond, the diapause induction rate is 100%, the storage period after the diapause can be 120 days, and eclosion adult Exorista civilis Rond can be obtained after 20 days when the diapause is removed. The method for adjusting the diapause of the Exorista civilis Rond has important significance for large-scale expanding propagation of the Exorista civilis Rond, extension of the shelf life of Exorista civilis Rond products and the field prevention and control action time of the Exorista civilis Rond products, improvement of pest control efficiency, deepening of understanding about the natural enemy insect development mechanism, and exploration of the adaptive mechanism of insects to the environment and the evolution path of the insects.

The invention discloses a method for improving the cold resistance of nasonia vitripennis. The method includes phases of 1), starting to regulate and rear hosts from adult stages, regulating illumination durations and temperatures on a daily basis and rearing the hosts until larvae are born; 2), regulating and rearing the larvae born by the hosts in the phase 1), regulating illumination durations and temperatures on a daily basis, selecting pupae formed in preset numbers of days at the phase and using the selected pupae as diapause hosts; 3), starting to regulate and rear the nasonia vitripennis from eclosion adult bee stages, arranging adult bees under dark conditions, regulating temperatures and rearing the adult bees for preset numbers of days; 4), selecting male and female adult bees paired in the phase 3) when the nasonia vitripennis lays eggs, and rearing the male and female adult bees in containers with the host pupae formed in the phase 2) according to a 1:1 proportion of adult bee pairs to the fly pupae; 5), regulating illumination durations and temperatures of rearing environments in the phase 4) on a daily basis, removing the adult bees after preset time elapses, allowing the host pupae to develop under identical conditions until larvae of the nasonia vitripennis develop.

The invention discloses beauveria bassiana granules. The beauveria bassiana granules are prepared through a method comprising the following steps that a fermentation culture medium is inoculated with beauveria bassiana or beauveria bassiana spores, wherein the fermentation culture medium is prepared through the method that 4 g of glucose, 0.4 g of monopotassium phosphate, 0.2 g of magnesium sulfate, 0.1 g of zinc sulfate, 8 mg of vitamin B1, 1,000 g of corn stalk particles and 1.2 liters of water are added into every one liter of the PD culture medium; fermentation is conducted for 7 days in the environment in which the temperature is 25 DEG C and the absolute humidity is 100%. The beauveria bassiana granules have the effects of being capable of adapting to a low-humidity and high-temperature environment in a field, efficiently infecting ostrinia nubilalis and efficiently killing pests; especially, the beauveria bassiana granules have a very good infection effect on diapause-relieving ostrinia nubilalis larvae, and therefore an important significance on long-term pest resisting in a corn production area is achieved; meanwhile, the beauveria bassiana granules belong to a biological preparation and have the advantages of being environmentally friendly and safe.

The invention discloses an efficient cultivation method of tussah cordyceps militaris. The method comprises the following steps: treating autumn cocoons which are in a diapauses period and are just subjected to cocooning and pupation at a temperature of 19-28 DEG C and humidity of 70-80% for 6-8 days, carrying out storing at 0-4 DEG C for 17-23 days, inoculating 0.3-0.7 ml of a cordyceps militarisliquid strain to the back parts of disinfected tussah living pupae from a third somite to a fifth somite, placing the tussah living pupae into a plastic disc, cultivating cordyceps militaris at proper temperature, humidity and illumination conditions for 45-50 days, and then carrying out harvesting after the cordyceps militaris is mature. Through the cultivation method provided by the invention,the grass yield of the tussah cordyceps militaris reaches 89.2%, the space utilization rate is increased by 56.7%, product quality, production efficiency and economic benefits are remarkably improved,and a technical guarantee is provided for industrialized production of the tussah cordyceps militaris, sufficient raw materials are provided for industries of food, medicine and health-care products,and the market application prospect is wide.

The invention belongs to the technical field of Raman spectrum detection and discloses a Raman spectrum model establishment method for rapid nondestructive identification on diapause termination silkworm eggs. The method comprises a Raman spectrum detection technique and a PLS_DA classification algorithm and mainly comprises steps of sample selection, data acquisition, data digging, conclusion analysis, and the like, wherein the step of sample selection comprises steps of selecting diapause eggs and diapause termination eggs of two varieties of Dazao and 9Fu*7Xiang; the step of data acquisition comprises steps of carrying out spectrum data acquisition by using a laser Raman spectrometer, processing Raman spectrum data, and establishing a PLS_DA qualitative analysis Raman spectrum model. Byadopting the method, diapause and diapause termination silkworm eggs can be rapidly and nondestructively distinguished, and identification analysis on nondestructive diapause termination household silkworm eggs can be finally achieved.

The invention discloses a diapause maintaining and cancellation method of Coccinella septempunctata. The method comprises the following steps: transferring Coccinella septempunctata entering diapause state to a condition of 18 DEG C and lighting period of L10:D14 (or dark) for storage; and after storage, transferring the Coccinella septempunctata to a condition of 24 DEG C and lighting period of L16:D8 to recover development. The method has diapause maintaining time of 114-183 days and diapause canceled in 7.52-8.62 days. The method is of great significance for large-scale propagating Coccinella septempunctata, prolonging shelf period and field prevention / control action time, grasping natural enemy insect development characteristic and occurrence dynamics, improving pest prevention / control efficiency, deepening understanding about natural enemy insect development mechanism, and investigating environment adaptability mechanism and evolution route of insect.