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Method for timely obtaining adult sericinus montelus gray by manually regulating pupa diapause

A technology of ribbon swallowtail and diapause, which is applied in the field of artificial breeding of resource insects, can solve the problems of waste, quantity dependence of ribbon swallowtail adults, artificial breeding dependence, etc., and achieves the effects of ensuring accuracy, low cost and simple process.

Inactive Publication Date: 2010-11-24
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the Ribbon Swallowtail is a monophagous butterfly, the artificial diet has not been successfully developed, and the artificial feeding is completely dependent on the natural host
During the growth and development season of the Ribbon Swallowtail, fully utilize the natural host to raise, and the Ribbon Swallowtail pupae obtained under the above-mentioned disclosed conditions are non-diapause pupae, which can only be stored at low temperature for a short period of time (usually a dormant state); if Obtain adult when there is no market demand, then completely waste; In addition, the amount of Ribbon Swallowtail adults obtained by the above-mentioned method depends on the number of host plants, and there is no cumulative effect

Method used

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  • Method for timely obtaining adult sericinus montelus gray by manually regulating pupa diapause
  • Method for timely obtaining adult sericinus montelus gray by manually regulating pupa diapause
  • Method for timely obtaining adult sericinus montelus gray by manually regulating pupa diapause

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The eggs of Ribbon Papilionaceae were collected and hatched at a temperature of 15-25°C and a light / dark cycle of 13 / 11-8 / 16 hours, and were raised with isolated leaves of horseshoe bells. Fresh isolated leaves of horseshoe bells were replaced and supplemented daily until pupation , almost all of the obtained Ribbon Papilio pupae were diapause pupae, and the length of the 9th segment of the abdomen of the diapause pupae was about 4 times that of the 3-day-old non-diapause pupae. Store the 3-15-day-old diapause pupae in full darkness at 4°C. After 30 days of storage, transfer the diapause pupae to 25°C with a light / dark cycle of 16 / 8 hours to eliminate diapause. 50% of the diapause pupae Adults will emerge from the 10th day to the 14th day ( figure 1 ).

Embodiment 2

[0031] According to the steps of Example 1, the difference is that the diapause pupae of the 3-15-day-old Swallowtail butterfly were stored at 0° C. in total darkness.

Embodiment 3

[0033] According to the steps described in Example 1, the difference is that the 3-15-day-old diapause pupae of the Ribbon Papilio was stored at 4° C. in total darkness for 60 days.

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Abstract

The invention belongs to the technical field of resource insect exploitation and utilization, and particularly relates to a method for timely obtaining adult sericinus montelus gray by manually regulating pupa diapause. The method is characterized by comprising the following steps of: when initially hatching the larvae, feeding sericinus montelus gray larvae under the periodic conditions of the normal temperature of 15 to 25 DEG C and short light / dark cycle of 8 / 16 to 12 / 12 hours to induce diapause of sericinus montelus gray pupae so as to obtain sericinus montelus gray diapause pupae; preserving the sericinus montelus gray diapause pupae for 15 to 30 days under the conditions of the temperature of 0 to 10 DEG C and complete darkness to promote the diapause development of the sericinus montelus gray diapause pupae; and transferring the sericinus montelus gray diapause pupae after low-temperature preservation to the conditions of the temperature of 25 to 28 DEG C and the light / dark cycle of 14 / 10 to 16 / 8 hours to terminate diapause. The method realizes centralized eclosion of the sericinus montelus gray diapause pupae within 4 to 5 days, and provides butterfly sources of timely butterfly flying for scenic spot building, celebration activities and the like.

Description

technical field [0001] The invention relates to the technical field of artificial cultivation of resource insects, in particular to a method for obtaining adults of the Ribbon Papilio butterfly by artificially regulating pupae diapause in good time. The method of the invention is a method for artificially controlling the diapause of the ornamental butterfly pupae and obtaining the adults of the Ribbon Papilio in good time, and provides a new way for the industrial development of the ornamental butterfly. Background technique [0002] The development of butterfly resources is currently a hot spot in the development of insect resources at home and abroad. Butterflies are brightly colored and loved by people. They are insect resources with great development value. They can be used as sightseeing resources and raw materials for making butterfly handicrafts. In recent years, the activity of releasing butterflies in foreign countries has gradually spread to our country. A Google s...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 王小平刘四周兴苗丁楠朱芬雷朝亮
Owner HUAZHONG AGRI UNIV
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