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Method for inducing diapause of Trichogramma ostriniae by constant temperature and storage method

A technology of Trichogramma corn borer and storage method, which is applied in animal husbandry and other fields, and can solve the problems of high production cost and long-term storage of Trichogramma and production scale.

Active Publication Date: 2008-12-24
DRY LAND FARMING INST OF HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the breeding technology of Trichogramma corn borer and other parasitic small eggs of Trichogramma corn borer and other parasitic Trichogramma in industrialized breeding has been relatively mature. The long-term storage of bee species has very urgent requirements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: Taking 1,000,000 Trichogramma corn borer as an example on July 10 this year, the method of breeding diapause Trichogramma corn borer is as follows:

[0013] 1. Breeding bees: First, on January 30, a sufficient amount of wheat moth eggs and seed bees (40g wheat moth eggs, 100,000 Trichogramma corn borer seed bees) were connected to the breeding beehive, and the temperature of the breeding beehive was controlled at The temperature is between 20-28°C, and the relative humidity is controlled between 70%-90%.

[0014] 2. Induce the diapause of Trichogramma corn borer: 24 hours after receiving the bees, blow off the seed bees, collect the eggs of the wheat moth and put them in the breeding room to continue to develop. On the third day after receiving the bees (about 50 hours after receiving the bees), put the Trichogramma bee into a shallow open bottle, seal it with a black cloth, and then transfer it to a controlled temperature of 10.5-12.5°C and a humidity of ...

Embodiment 2

[0017] Example 2: The basic conditions and steps of the constant temperature induced Trichogramma corn borer diapause storage method are the same as in Example 1, wherein the storage conditions of the induced diapause Trichogramma is a temperature of 3-5 °C, and the storage time is 5 months . The conditions for the long-term storage of Trichogramma to recover and develop are as follows: temperature 28°C, humidity 90%, photoperiod 14L:10D.

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Abstract

The invention provides a constant-temperature inducing maize-borer oophagous trichogrammae diapause storage method. The collected maize-borer oophagous trichogrammaes which have developed to the ovum telophase from the middle larva stage adopt constant-temperature inducing maize-borer oophagous trichogrammae diapause, and are stored under a specific temperature and humidity, and the development of the maize-borer oophagous trichogrammae is restored by changing the prior temperature to use. The method is applicable to oophagous trichogrammae breeding by using small gelechiid ovum; the technology matches large-scale oophagous trichogrammae production; the method is applicable to long-term production and storage of maize-borer oophagous trichogrammaes; thereby, sufficient maize-borer oophagous trichogrammaes are prepared for pest control, and the method can also be used to store breeding maize-borer oophagous trichogrammaes.

Description

technical field [0001] The invention relates to a diapause storage method for insects, in particular to a long-term storage method for the dominant parasitic natural enemy of corn borer, Trichogramma corn borer. Background technique [0002] The corn borer Trichogramma (Trichogramma ostriniae Pang et Chen) is the dominant natural enemy of corn borer, the main pest of corn borer, in most areas of my country. Low-cost, large-scale production of this bee species and its use in the control of corn borer can effectively control the pest damage and improve my country's grain production. Yield. At present, my country has successfully realized the breeding of Trichogramma corn borer with wheat moth eggs or rice moth eggs. In view of the poor storage resistance of conventionally propagated Trichogramma, the characteristics of storage resistance after Trichogramma diapause were used to induce Trichogramma corn borer. Bee diapause and long-term storage is one of the best ways to prolong...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 郑礼宋凯毛文杰刘冬郑书宏
Owner DRY LAND FARMING INST OF HEBEI ACAD OF AGRI & FORESTRY SCI
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