11 results about "Rice moth" patented technology

The invention discloses a method for cultivating trichogramma ostriniae in vitro, and belongs to the field of insect feeding. The artificial host ovum comprises a sealed hemisphere prepared from a plastic penetrating material, an oviposition attractant coated on the outer convex surface of the hemisphere and an internal artificial culture solution, the artificial culture solution is a tussah egg solution. According to the method, the tussah egg liquid is used for cultivating the trichogramma ostriniae, and the effect of reducing the breeding cost can be achieved. The body length of the trichogramma ostriniae cultivated by the method is increased by about 21.4% compared with that of trichogramma ostriniae bred by rice moth eggs; the average brood amount is increased by about 24.9%; the parasitic rate is obviously higher than that of trichogramma ostriniae bred by rice moth eggs. The biological control effect on the trichogramma ostriniae is remarkably improved.

The utility model provides a kind of loaded pheromone trapping device. A kind of loaded pheromone trapping device, comprising: mounting plate;Trapping cylinder is set to the top of mounting plate, trapping cylinder includes installation cylinder, opening hole, cone bucket, taper slot and opening slot. The utility model provides a kind of loaded pheromone trapping device, by pushing pull rod to drive slide bar and extrusion plate to move downwards, extrusion plate moves downwards inside trapping cylinder, the two species of rice moth received in trapping cylinder is rolled and crushed, can wait for a long time to disassemble trapping cylinder and extrusion device to clean, the device simple structure, practicality is strong, can effectively reduce the labor intensity of operator, improve the elimination efficiency of two species of rice moth simultaneously, reduce the accumulation of a large number of two species of rice moth in trapping cylinder Influence the volatilization efficiency of induction core, prolong the service life of pheromone in induction core, improve the trapping efficiency of trapping device, fast efficiency capture pest, improve the practicality of device.

The invention discloses a corcyra cephalonica breeding method which comprises the following steps: from the day of inoculation of corcyra cephalonica eggs, the corcyra cephalonica eggs are fed with feed for five times, the feeding time points are respectively the 10th-14th day, the 17th-21st day, the 24th-28th day, the 31st-35th day and the 38th-42nd day after inoculation, the feeding amount of the third feeding is the maximum value of all the five feeding amounts, and the feeding amount of the fourth feeding is the maximum value of all the five feeding amounts. The ratio of the third feeding amount to the second feeding amount is 1.4-1.6, and the ratio of the third feeding amount to the fourth feeding amount is 1.2-1.3. According to the method provided by the invention, the rice moths grow relatively early, the moth yield and the egg laying amount are high, meanwhile, the problems of unbalanced nutrient feeding of larvae, diapause, low yield and the like in the feeding process are avoided, and large-scale production of the rice moths is facilitated.

The invention discloses a preparation method of chromosomes of tiny insects trichogramma dendrolimus, and belongs to the technical field of chromosome preparation. Comprising the following steps: (1) preparing a pre-pupa material: selecting corcyra cephalonica eggs parasitized by trichogramma dendrolimus for 4-8 days, and cutting out a trichogramma dendrolimus pre-pupa; (2) pretreatment: pretreating the pre-pupa material in a colchicine solution; (3) low-permeability treatment: carrying out low-permeability treatment on the pretreated material in a low-permeability solution; and (4) fixing, uncovering, dyeing and sealing the material subjected to the low-permeability treatment. The invention provides a simpler trichogramma dendrolimus chromosome slide preparation method, chromosome slides with clear forms can be prepared in batches, the dispersion degree of chromosomes is large, the length, centromere position, arm ratio and other characteristics of the chromosomes can be observed more clearly, the chromosome characteristics are determined through karyotype analysis, and the chromosome slide preparation method is suitable for large-scale production. And a foundation is laid for cell level research and genomics deep research of trichogramma dendrolimus.

This invention discloses an antibacterial and antiviral coating based on *Pseudomonas aeruginosa* oil, its preparation method, and its application, belonging to the technical field of antibacterial and antiviral coatings. The coating comprises *Pseudomonas aeruginosa* oil, nano-silver, chitosan, and polylactic acid. The preparation method involves first dissolving polylactic acid and chitosan separately, then mixing and adding nano-silver and *Pseudomonas aeruginosa* oil, followed by stirring and degassing. This invention combines *Pseudomonas aeruginosa* oil rich in unsaturated fatty acids with nano-silver and chitosan in a polylactic acid film-forming matrix. Utilizing the destructive effect of *Pseudomonas aeruginosa* oil on viral envelopes, the rapid bactericidal effect of nano-silver, and the auxiliary antibacterial effect of chitosan, the three components synergistically enhance the coating's highly efficient and broad-spectrum killing and inhibitory capabilities against common pathogens and viruses, while also exhibiting good biosafety and environmental friendliness.

The utility model belongs to insect artificial breeding technical field, concretely relates to a box drawer type device of rice moth feeding and oviposition integration, including vertical cabinet, three layers of drawers are arranged in vertical cabinet, top layer is adult catching drawer, middle is larva feeding drawer, bottom layer is adult oviposition drawer, larva feeding drawer has bottom, top is open, the top of adult catching drawer is gauze, bottom is the net board of pluggable, when the net board in adult catching drawer is drawn out, adult catching drawer and larva feeding drawer intercommunicate, when the net board is inserted into adult catching drawer, can intercept rice moth adult between gauze and net board, when adult catching drawer shifts to bottom layer, replaces adult oviposition drawer, the below of adult oviposition drawer is provided with insect egg collection unit, through the structural design of " larva feeding drawer is above, adult catching drawer is below", utilizes the habit of adult insect flying upward to light, makes it automatically fly into the adult catching drawer (gauze place) of feeding drawer from above after eclosion.

The invention discloses an artificial feed for corcyra cephalonica. The artificial feed comprises the following components in parts by weight: corn flour, wheat bran, white granulated sugar, yeast, vitamin C, inositol, cholesterol, choline chloride, multivitamins, Webster salt and water and does not contain soybean flour. The feed formula provided by the invention can shorten the initial moth seeing period of the rice moths and improve the breeding efficiency, and is beneficial to large-scale production of the rice moths.

The invention provides a method for identifying ritadine from a host of Noctuid black egg bees and breeding the Noctuid black egg bees on a large scale, and belongs to the technical field of biological prevention and control of agricultural pests. The invention provides a noctuid black egg bee host recognition ritadine, which is obtained by treating spodoptera frugiperda eggs with n-hexane and separating a liquid phase. According to the present invention, the Noctuid black-egg bee host recognition ritamine can domesticate Noctuid black-egg bees and improve the parasitism rate of corcyra cephalonica eggs and the eclosion rate of offspring bees, and the results of the embodiment show that after the Noctuid black-egg bees are domesticated, the parasitism rate of the F30 generation is 88.23%, the eclosion rate of the offspring bees is 89.92%, and the large-scale breeding of the Noctuid black-egg bees can be achieved.

The invention discloses a method for improving parasite efficacy of beetles to corcyra cephalonica eggs by using noctuid scale. The invention finds that the nedoptera can improve the parasitic ability of the abdominal cocoon bees, and provides a rice moth egg for improving the parasitic ability of the abdominal cocoon bees, and a preparation method of the rice moth egg comprises the following steps: 1) collecting the nedoptera, namely the nedoptera, the nedoptera and the rice moth egg; and 2) uniformly mixing the gray wing noctuid scale hair and the rice moth eggs to obtain the rice moth eggs. The gray wing moth scale hair comprises grassland spodoptera scale hair and / or prodenia litura scale hair. According to the method disclosed by the invention, the rice moth eggs are prepared from the noctuid scale, so that the parasitic rate of the abdominal cocoon bees to rice moth egg masses is increased from about 36% to more than 80%, the breeding cost can be reduced, the labor is saved, and the efficient breeding of the abdominal cocoon bees is realized.

The invention discloses a trichogramma gene editing method. The method comprises the following steps: (1) preparing a test bee species and hosts: preparing host tussah eggs; trichogramma is inoculated, and the trichogramma is inoculated for 6-8 hours; the parasitized tussah eggs are clamped in a fruit fly tube to be cultured; (2) preparation of pupae for injection: culturing in an incubator for 9-10 days, and splitting tussah eggs to obtain female pre-pupae of trichogramma; (3) preparing a solution for injection: mixing Cas9 and sgRNA, and incubating to form an RNP compound; (4) micro-injection: injecting the solution into hemolymph; (5) culturing female pupae in vitro after injection; (6) sub-packaging single heads of the eclosion female bees into finger-shaped tubes, and putting corcyra cephalonica egg cards for parasitizing until the offspring drones are all eclosion; and (7) screening mutants: observing mutation phenotypes under a stereoscope, and determining that gene editing succeeds through PCR (Polymerase Chain Reaction) detection. According to the method, the hosts are replaced by the tussah eggs from the rice moth eggs, so that mechanical damage caused in the dissecting process is reduced.

This invention discloses a method for raising *Spodoptera litura* (side-necked bugs). Instead of *Spodoptera litura*, it uses artificially fed larvae such as mealworms, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae. Sufficient feed ensures a good food source for the bugs, and the availability of feed is higher than that of fresh leaves, effectively avoiding the risk of virus transmission from fresh leaves. The feeding process is also simpler and easier to operate. The bugs are fed a nutrient solution in conjunction with the feed. Using honey water, glucose, and mixed juice in the nutrient solution as attractants can induce feeding. Amino acid powder can provide sufficient nutrients for the side bug, resulting in a high survival rate and large egg production, which is beneficial for the large-scale propagation of the side bug. Feeding the side bug with a mixture of mealworm, rice moth larvae, citrus fruit fly larvae, and beet armyworm larvae can provide more comprehensive nutrition, shorten the development period, increase the survival rate of each developmental stage, and extend the survival time of adults, which can effectively increase the egg production of the side bug.