332 results about "Spodoptera" patented technology

The invention relates to the field of biological control, and discloses screening of entomogenous fungi with high pathogenicity to prodenia litura and a compound application of the entomogenous fungi and tetraniliprole. The beauveria bassiana strain with high toxicity to the prodenia litura is collected from a tobacco field in polder town in Jianghua County, Yangzhou City, Hunan Province. The invention further provides an efficient, pollution-free and residue-free insecticide combining the tolfenpyrad and the beauveria bassiana, and the insecticide has a synergistic effect. Research on the compounding effect of the tetratolfenpyrad and the beauveria bassiana shows that the insecticide can show a remarkable synergistic effect after being compounded, the prevention and treatment effect is improved, the use dosage of a single chemical pesticide can be obviously reduced, environmental pollution is reduced, and the problems that an existing pesticide is prone to generating resistance, not obvious in pesticide effect and the like are effectively solved.

The present invention relates to the field of microbial culture, in particular to a method for expanding propagation and producing spores of Metarhizium rileyi and use thereof. In the present invention, Metarhizium rileyi Mr006 is sprayed on 2nd instar live larvae of Spodoptera litura treated with a sub-lethal dose of chlorantraniliprole to cause infection, the 2nd instar larvae of the Spodoptera litura infected with the Metarhizium rileyi Mr006 spores are concentrated into a sterile box, placed into an artificial climate box and continuously fed with an artificial feed until death or pupation, the infection condition of the insect bodies is observed, spores are collected after a 1st complete spore production, after the infected insects are subjected to moisture preservation, expanded propagation and culture are continuously performed, and the spores are repeatedly collected twice to obtain the maximum spore yield.

The invention belongs to the technical field of pesticides, and particularly relates to a mesoionic pyrimidinium compound, and an N-oxide or salt and application thereof. The compound is shown as a general formula I, wherein X1 and X2 respectively and independently represent hydrogen, halogen, cyano, hydroxyl, amino, alkyl or halogenated alkyl; y1, Y2, and Y3 each independently represent hydrogen, halogen, or the like; q1 and Q2 respectively and independently represent CH2, O or S, and at least one of Q1 and Q2 is O; r1 and R2 respectively and independently represent halogen. The compound has an excellent prevention and treatment effect on pests such as spodoptera frugiperda and armyworm.

The invention discloses application of a microbial natural product GameXPeptide-A in improving the pest control effect of an insecticide, and belongs to the technical field of biological control. Experiments prove that after the GameXPeptide-A is combined with insecticides such as metarhizium anisopliae, beauveria bassiana, nuclear polyhedrosis virus, 4% of abamectin acetamiprid, 10% of spinosad emamectin benzoate, 30% of etoxazole nitrile, 45% of biphenyl etoxazole, 5% of emamectin benzoate, 5% of chlorantraniliprole, 5% of abamectin and the like for use, the insecticidal effect is obviously improved; the insecticidal activity of the insecticide on agricultural pests such as thrips, ostrinia nubilalis and prodenia litura can be effectively improved, so that the substance can be combined with different insecticides for use, and the insecticidal activity of the insecticide on crop pests in agricultural production such as lepidoptera, diptera, coleoptera, orthoptera, hemiptera and homoptera is improved; the compound can be used for development and application of novel pest immunosuppressants or insecticide synergists.

The invention discloses an indole sophocarpidine derivative photoaffinity active molecular probe which is formed by connecting an active group and a reactive group, the active group is a sophocarpidine derivative, the reactive group is a substance containing a diaziridine group and an alkyne group, and the chemical formula of the sophocarpidine derivative is as shown in the formula I. The invention further discloses a preparation method of the indole sophocarpidine derivative photoaffinity active molecular probe. After Spodoptera frugiperda Sf9 cells are treated by the indole sophocarpidine derivative photoaffinity active molecular probe for 72 hours, the inhibition rate can reach 86.33%, potential target protein when the sophocarpidine derivative acts on the Sf9 cells can be determined by utilizing the indole sophocarpidine derivative photoaffinity active molecular probe, targeted modification of sophocarpidine is facilitated, and the application range of the indole sophocarpidine derivative photoaffinity active molecular probe is widened. Further, matrine pesticides with excellent insecticidal activity are efficiently synthesized, and agricultural pests are better prevented and controlled.

The invention belongs to the field of microbial natural product pesticides, and particularly discloses a polyketone compound as well as a preparation method and application thereof. The compound disclosed by the invention is prepared from a fermentation crude extract of a strain of tobacco endophytic fungus Aspergillus japonicus TE-739D, and the compound disclosed by the invention is prepared from the fermentation crude extract of the tobacco endophytic fungus Aspergillus japonicus TE-739D. The chemical structure of the compound is identified through a modern spectroscopy technology, such as a high resolution mass spectrum HRESIMS and a superconducting nuclear magnetic resonance spectrum NMR, and the compound is named as japonidiene C. The invention further discloses a preparation method of the compound. The polyketone compound japondiene C has an obvious insecticidal activity on 2-instar larvae of spodoptera frugiperda, and can be used for preventing and treating the second instar larvae of the spodoptera frugiperda.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus thuringiensis ZR-560 and application thereof, the bacillus thuringiensis ZR-560 is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.30586. The invention further discloses a preparation method of the bacillus thuringiensis ZR-560. The bacillus thuringiensis ZR-560 strain provided by the invention has high insecticidal toxicity, and the parasporal crystal protein preparation of the bacillus thuringiensis ZR-560 strain has a corrected death rate of 100% for spodoptera frugiperda, ostrinia nubilalis, prodenia litura and beet armyworm, and is a Bt strain with a relatively broad-spectrum insecticidal effect.

The invention belongs to the technical field of pesticides, and particularly relates to a mesoionic pyrimidinium compound, and an N-oxide or salt and application thereof. The compound is shown as a general formula I, wherein Q1 and Q2 respectively and independently represent O or S; x represents a cyano group, a cyano alkyl group, an aryl group or a heterocyclic group; y1, Y2, Y3, Z1, Z2, R1, R2, R3, R4, R5, R6, R7, and R8 independently represent hydrogen, halogen, a cyano group, a nitro group, an alkyl group, etc. The compound has an excellent prevention and treatment effect on pests such as spodoptera frugiperda, armyworm and prodenia litura.

The invention belongs to the technical field of pesticides, and particularly relates to a mesoionic pyrimidinium compound, and an N-oxide or salt and application thereof. The compound is shown as a general formula I, wherein X and Z respectively and independently represent hydrogen, alkyl, alkenyl, alkynyl, naphthenic base and the like; y represents chlorine or bromine. The compound has an excellent prevention and treatment effect on pests such as spodoptera frugiperda, armyworm and prodenia litura.

The invention provides metarhizium ledebusii GZFW2211 and an application of the metarhizium ledebusii GZFW2211. The green muscardine fungus GZFW2211 is preserved in Guangdong Microbial Culture Collection Center on February 13, 2025, and the preservation number is GDMCC No: 65784. The spodoptera frugiperda spore suspension is a strain separated from spodoptera frugiperda larvae, the strain is high in growth speed, short in conidium production time and large in spore production quantity, and the prepared spore emulsion suspension is high and stable in pathogenicity to important pests such as spodoptera frugiperda, prodenia litura and agrotis ypsilon. The insecticidal composition can be widely used for preventing and treating pests such as spodoptera litura, prodenia litura and agrotis ypsilon.

The invention belongs to the technical field of pesticides, and particularly relates to a mesoionic pyrimidinium compound, and an N-oxide or salt and application thereof. The compound is shown as a general formula I, wherein Q1 and Q2 respectively and independently represent O or S; x represents a cyano group, a cyano alkyl group, an aryl group or a heterocyclic group; y represents hydrogen, an alkyl group, an alkenyl group, an alkynyl group or the like; and Z1, Z2, R1, R2, R3, R4, R5, R6, R7 and R8 independently represent hydrogen, halogen, cyano, nitro, alkyl and the like. The compound has an excellent prevention and treatment effect on pests such as spodoptera frugiperda, armyworm and prodenia litura.

The invention discloses a pepper field prodenia litura adult trapping and killing device and method. The pepper field prodenia litura adult trapping and killing device comprises an AI adult prediction system, an insect trapping device, a high-voltage power grid killing device, an insect corpse collecting device and an energy supply device. The AI adult prediction system is in communication connection with the insect trapping device and is used for monitoring prodenia litura adults in real time and sending a starting signal to the insect trapping device; the insect trapping device carries a prodenia litura sex attractant and is used for releasing the prodenia litura sex attractant to trap adults of prodenia litura after receiving the opening signal; the high-voltage power grid killing device is used for killing adults through cooperation of contact electric shock and air electric shock; the insect corpse collecting device is arranged below the high-voltage power grid killing device and is used for collecting killed insect corpses; the energy supply device is electrically connected with the AI adult prediction system, the insect trapping device and the high-voltage power grid killing device and supplies power to all electric components. Non-chemical, accurate, environment-friendly and efficient trapping and killing of the prodenia litura adults in the pepper field and stable and long-acting operation of the device are realized.

The invention belongs to the technical field of pesticides, and particularly relates to a substituted benzisoxazole compound, a preparation method thereof, an insecticidal composition and application. The compound is shown as a general formula I, wherein Z1, Z2, Z3, Z4 and Z5 independently represent N or CR; r independently represents hydrogen, halogen, alkyl, alkenyl or the like; x represents an alkyl group, a haloalkyl group, an alkenyl group or the like; y represents alkyl. The compound has excellent prevention and treatment effects on pests such as spodoptera frugiperda, armyworm, plutella xylostella and prodenia litura.

The invention discloses compound application of entomogenous fungi with high pathogenicity to prodenia litura and thymol. The compounded insecticide is prepared by combining a beauveria bassiana strain with the preservation number of GDMCC (China General Microbiological Culture Collection Center) NO: 65072 and thymol. The research on the compounding effect of thymol and beauveria bassiana shows that the compounded insecticide can show a remarkable synergistic effect, the prevention and treatment effect is improved, the use dosage of a single chemical pesticide is reduced, the environmental pollution is reduced, and the problems that the existing pesticide is easy to generate resistance, the pesticide effect is not obvious and the like are effectively solved.

The invention belongs to the technical field of biological control, and relates to a preparation method of prodenia litura nuclear polyhedrosis virus concentrate, which comprises the following steps: cultivating and hatching prodenia litura eggs at constant temperature, culturing the prodenia litura eggs in a dark place into 4-day-old larvae, performing virus infection and proliferation on the 4-day-old larvae, collecting and screening dead insects with strong toxicity and early death as virus semi-finished products, and refrigerating and storing the virus semi-finished products. And finally, preparing the virus semi-finished product into mother powder to obtain the prodenia litura nuclear polyhedrosis virus mother drug. The method comprises the following steps: extracting a parent drug sample to obtain a virus polyhedrosis, extracting a DNA comparison gene sequence of the virus polyhedrosis, prodenia litura nuclear polyhedrosis virus which is identified as prodenia litura nuclear polyhedrosis virus when the similarity between the virus polyhedrosis and Spodoptera litura Nucleoplydrovirus reaches 80% or above, and measuring various performance indexes, so that the prodenia litura nuclear polyhedrosis virus can be identified as prodenia litura nuclear polyhedrosis virus. The prodenia litura nuclear polyhedrosis virus mother drug prepared by the preparation method is used for preventing and treating lepidoptera pests such as prodenia litura, has important significance, has good development and application prospects, and has application value in preparation of insecticides and prevention and treatment of flat thorn moths.

The utility model relates to a trapping device for grassland spodoptera exigua. The trapping device comprises a first trapping mechanism and a second trapping mechanism, wherein the top of the first trapping mechanism is sleeved with the second trapping mechanism in a sliding mode; the first trapping mechanism comprises a hollow first barrel, an inlet is formed in the side portion of the first barrel, a one-way insect inlet assembly is installed at the inlet, a tray is arranged in the first barrel, and attractant is loaded in the tray; the second trapping mechanism comprises a hollow second cylinder body, a trapping lamp is arranged at the top of the second cylinder body, an electric insect net is arranged at the bottom of the second cylinder body, and after being attracted by the attractant to enter the first cylinder body in a one-way mode, the grassland spodoptera exigua is attracted to the position near the second cylinder body through the trapping lamp and touches the electric insect net so as to be killed through electric shock. According to the grassland spodoptera frugiperda trapping device, the trapping effect on grassland spodoptera frugiperda is effectively improved, the first barrel and the second barrel allow the second barrel to be contained in the first barrel through sliding sleeving, the problem that a traditional trapping device is lack of portability due to curing installation is solved, and the grassland spodoptera frugiperda trapping device can be moved and contained and is convenient and practical.

The invention discloses an Acinetobacter baumannii H5 strain and application thereof, the Acinetobacter baumannii H5 strain is preserved in the Guangdong Microbial Culture Collection Center on September 29, 2024, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 65133; and the strain has a good degradation effect on polyethylene glycol terephthalate plastics. As the spodoptera frugiperda is a recently occurring major invading pest, domestic and overseas scholars are very limited in research on intestinal microorganisms of the spodoptera frugiperda, no related report about that intestinal strains of the spodoptera frugiperda can effectively degrade polyethylene glycol terephthalate exists at present, and the research on the degradation mechanism of the spodoptera frugiperda is also relatively lacked. The Acinetobacter baumannii H5 can enrich a plastic degradation microorganism germplasm resource library, provides the most basic resource for the technical breakthrough of polyethylene glycol terephthalate biodegradation, and also can optimize the ecological environment protection.

The invention relates to the technical field of spodoptera frugiperda repellent strains, in particular to a spodoptera frugiperda egg surface bacterium separation and culture method and spawning repellent application, and is characterized by comprising the following steps: S1, feeding to-be-tested insects; s2, disinfecting spodoptera frugiperda pupae; s3, culturing and collecting an egg surface microorganism fermentation solution without environmental bacteria; s4, preliminary purification and separation identification of ovum surface microorganisms; s4-1, screening and purifying the strain; s4-2, separation and identification of spodoptera frugiperda egg surface microorganisms; s5, detecting the oviposition avoiding activity of the lactobacillus; s5-1, planting the plants; s5-2, determining the avoiding activity by a spawning cage method; and S5-3, performing field simulation determination on the spawning avoiding activity. According to the invention, the condition that the lactobacillus on the spawning surface of the spodoptera frugiperda can generate obvious spawning avoiding activity on the spodoptera frugiperda under indoor and field simulation conditions is clear, the positive effect on biological control of the spodoptera frugiperda is achieved, the drug resistance of the spodoptera frugiperda to drugs can be reduced, a strategy is provided for pest control, and a certain theoretical basis is provided for biological control.

The present invention relates to a method of combating and / or controlling a pest selected from the Noctuidae family, which comprises applying to said pest, to a locus of said pest, to a plant susceptible to attack by said pest, or to a plant propagation material susceptible to attack by said pest, a pesticidal composition comprising isocycloseram as active ingredient A, and emamectin as active ingredient B.

The invention discloses a prodenia litura nuclear polyhedrosis virus SpltMNPV-c5 and an application of the prodenia litura nuclear polyhedrosis virus SpltMNPV-c5. Relates to the technical field of biological control. According to the invention, SpltMNPV-c 5 is processed into a preparation for preventing and treating lepidoptera pests such as prodenia litura, and the preparation has important significance; according to the invention, mother powder prepared by SpltMNPV-c 5 is used as an effective component, an optimal formula is determined by measuring wetting time, suspension rate and the like of a carrier, an auxiliary agent and an ultraviolet protective agent, the carrier, the auxiliary agent and the ultraviolet protective agent are mixed according to a ratio to prepare the wettable powder, and various performance indexes are measured; the virulence to prodenia litura is measured by a leaf soaking method and an insect soaking method, so that a theoretical basis is provided for developing SpltMNPV-c5 into a preparation for preventing and treating prodenia litura.

The present invention belongs to the technical field of pesticides, and particularly relates to a mesoionic pyrimidinium compound, an N-oxide or salt thereof and the use thereof. The compound is as shown in general formula (I), wherein X represents cyano, cyanoalkyl, aryl, or heterocycly; Q1 and Q2 each independently represent O or S; M1, M2, M3, M4 and M5 each independently represent CR9 or N, and at least two of them are N; R1, R2, R3, R4, R5, R6, R7, R8 and R9 each independently represent hydrogen, nitro, amino, cyano, halogen, etc. The compound has an excellent control effect on pests such as Chilo suppressalis, Spodoptera frugiperda, Mythimna separata, Spodoptera litura, Aphis craccivora, rice planthopper, etc., and is safe for bees.

The invention relates to the technical field of microorganisms, in particular to serratia marcescens and application of the serratia marcescens in prevention and treatment of lepidoptera pests. The serratia marcescens CB08 provided by the invention is preserved in the China Center for Type Culture Collection, and the preservation number of the serratia marcescens CB08 is CCTCC (China Center for Type Culture Collection) NO: M 2025266. The strain has a remarkable prevention and control effect on Lepidoptera pests such as spodoptera frugiperda, armyworm, prodenia litura and cotton bollworm, has no pathogenicity on host crops such as corn, is a bacterium with excellent Lepidoptera pest prevention and control potential, has a very wide application prospect, and is expected to bring a new solution to the field of agricultural pest prevention and control.

The present invention provides an isoxazoline-substituted benzamide compound and a stereoisomer and agriculturally or veterinarily acceptable salt thereof. The compound is as represented by formula (I). In the formula, X represents hydrogen, halogen, hydroxyl, alkoxy, or alkylthio; Y is hydrogen, halogen, alkyl, or haloalkyl; Z is haloalkyl or haloalkoxy; M is CH or N; Q is hydrogen or halogen; R1 is hydrogen, halogen, hydroxyl, cyano, amino, nitro, formyl, cyanoalkyl, hydroxyalkyl, alkyl, etc.; and R2 is hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, etc. The compound has an excellent control effect on pests such as Spodoptera frugiperda, Myzus persicae, Nilaparvata lugens, Tetranychus truncates, and Bradysia odoriphaga.

The invention belongs to the technical field of insect feeding, and particularly relates to artificial feed for spodoptera frugiperda. Comprising the following raw materials in parts by mass: 10 to 30 parts of soybean meal, 30 to 50 parts of wheat germ, 70 to 100 parts of wheat bran, 10 to 50 parts of corn flour, 20 to 50 parts of crushed corn leaves, 15 to 40 parts of beer yeast powder, 5 to 30 parts of five-vitamin glucose, 5 to 20 parts of casein, 5 to 15 parts of ascorbic acid, 25 to 35 parts of agar powder, 0.5 to 2.0 parts of choline, 1.0 to 3.0 parts of sorbic acid, 0.1 to 0.5 part of inositol, 0.05 to 0.20 part of streptomycin sulfate, 1.0 to 3.0 parts of methyl parahydroxybenzoate, 15 to 30 parts of konjaku flour, 15 to 35 parts of milk powder, 0.2 to 2.0 parts of vitamin B6 and 1.0 to 1.0 part of corn germ oil. 5.0 parts of water and 1000 to 1500 parts of water. The artificial feed can significantly shorten the developmental duration of spodoptera frugiperda and improve the pupation rate and eclosion rate.

The invention discloses application of cordyceps javanica Bd01 and a dispersible oil suspending agent thereof to prevention and control of spodoptera frugiperda, and relates to the technical field of biology. The research finds that the cordyceps javanica Bd01 has a strong infection lethal effect on larvae of spodoptera frugiperda at all ages, the pupation rate and eclosion rate of the larvae can be remarkably reduced, and the breeding cycle of pests can be effectively blocked. The invention further develops a dispersible oil suspending agent, and the suspending rate is effectively improved through formula optimization. The dosage form takes soybean oil as a dispersion medium, does not contain harmful solvents such as methylbenzene, is free of dust pollution and is friendly to operators and the environment, and ascorbic acid is added to improve the ultraviolet resistance of spores and prolong the effective period of the pesticide effect. In addition, the preparation also has a certain control effect on caffeinidae, is wide in application scene, provides a new scheme for green control of spodoptera frugiperda, solves the problems of chemical pesticide resistance and residue, and accords with the development requirements of green agriculture.

The invention discloses application of an epidermal protein gene SlituCP26 in prevention and treatment of prodenia litura, and relates to the technical field of biology. The nucleotide sequence of the SlituCP26 gene is shown as SEQ ID NO: 1, the SlituCP26 gene is highly expressed in an indoxacarb resistant strain, dsRNA of the gene is prepared, the dsRNA is micro-injected into prodenia litura larvae, after injection, the expression level of the SlituCP26 gene is obviously reduced, the body type of the larvae is obviously reduced, the epidermis thickness is obviously reduced, and the sensitivity to indoxacarb is obviously improved. The invention also provides a SlituCP26 gene promoter, and the drug resistance of the prodenia litura can be detected through the difference in sequence after the promoter is amplified. According to the invention, the fact that the epidermal protein SlituCP26 gene participates in the effect of the prodenia litura on resisting insecticides and the formation process of a resistance mechanism is clear, and the epidermal protein SlituCP26 gene has theoretical significance on the development of new insecticides.

The invention belongs to the field of microorganisms, and particularly discloses Aspergillus nomiae for preventing and controlling spodoptera frugiperda. The technical problem to be solved by the invention is how to prevent noctuid insects from harming plants. The invention provides aspergillus for preventing and treating noctuid insects from harming plants, the aspergillus is Aspergillus nomiae WNAN1, and the preservation number of the aspergillus nomiae is CGMCC (China General Microbiological Culture Collection Center) No.41741. The invention further provides a preparation method of the aspergillus nomiae. Experiments prove that the aspergillus has a relatively high fatality rate on third-instar larvae of the spodoptera frugiperda, and can be used for preventing and controlling the spodoptera frugiperda.

The invention relates to the field of chemical engineering and pesticides, in particular to an isoxazoline derivative containing dihydrazide as well as a preparation method and application of the isoxazoline derivative. The bishydrazide-containing isoxazoline derivative is a compound as shown in a formula (1). The novel isoxazoline derivative containing dihydrazide is obtained by combining an isoxazoline structure and a dihydrazide structure and carrying out special structural design, and the isoxazoline derivative containing dihydrazide has no cross resistance with existing insecticides, is relatively low in toxic and side effects, and can be applied to the field of pesticides. Meanwhile, the insecticidal composition has broad-spectrum insecticidal activity and an excellent insecticidal effect, and the problem that the pesticide resistance of pests such as spodoptera frugiperda and plutella xylostella is enhanced at present is effectively solved. Formula (1) # imgabs0 #

Methods for controlling plant pests are disclosed. In particular, insecticidal proteins having toxicity to Spodoptera insect pests are provided. Polynucleotides comprising codons optimized for expression in plants encoding the insecticidal proteins are also provided. Methods of making the insecticidal proteins and methods of using the insecticidal proteins and polynucleotides encoding the insecticidal proteins of the invention, for example in transgenic plants to confer protection from insect damage, are also disclosed.

The present invention provides a gene derived from Spodoptera exigua with antiviral effect and its application. The amino acid sequence of the encoded protein is SEQ ID NO:1, and the nucleotide sequence is SEQ ID NO:2. The present invention also provides a method for increasing the sensitivity of Spodoptera exigua to Spodoptera exigua nuclear polyhedrosis virus, which is to reduce the expression level of the gene in Spodoptera exigua. The present invention has screened a gene with antiviral effect from the important agricultural pest Spodoptera exigua, analyzed its sequence structure and spatio-temporal expression pattern, and at the same time used overexpression and RNA interference techniques to prove that the gene has an effect against Spodoptera exigua nuclear polyhedrosis virus, providing a new target for better controlling Spodoptera exigua with viruses in production and having good application prospects.