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The invention provides for methods of manufacturing an oligonucleotide comprising a pentavalent phosphate triester. In particular, the method comprises providing a 5′ blocked-nucleoside, deblocking the 5′ blocked-nucleoside to form a 5′ OH-nucleoside, coupling the 5′ OH-nucleoside with a phosphoramidite to form and oligonucleotide comprising a trivalent phosphite triester; and oxidizing the oligonucleotide comprising a trivalent phosphite triester to the oligonucleotide comprising a pentavalent phosphate triester. In some embodiments, the wash between any of the steps above is with at least one solvent wash comprising a toluene.

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

The present invention features novel compositions, linkers, derivatized solid supports, and methods for the efficient solid phase synthesis of oligonucleotides, including RNA, DNA, RNA-DNA chimeras, and analogs thereof.

The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain poly unsaturated fatty acids.

A process and apparatus for producing a synthesis gas for use as a gaseous fuel or as feed into a Fischer-Tropsch reactor to produce a liquid fuel in a substantially self-sustaining process. In one embodiment, a slurry of particles of carbonaceous material in water, and hydrogen from an internal source, are fed into a hydro-gasification reactor under conditions whereby methane rich producer gases are generated and fed into a steam pyrolytic reformer under conditions whereby synthesis gas comprising hydrogen and carbon monoxide are generated. A portion of the hydrogen generated by the steam pyrolytic reformer is fed through a hydrogen purification filter into the hydro-gasification reactor, the hydrogen therefrom constituting the hydrogen from an internal source. The remaining synthesis gas generated by the steam pyrolytic reformer is either used as fuel for a gaseous fueled engine to produce electricity and / or process heat or is fed into a Fischer-Tropsch reactor under conditions whereby a liquid fuel is produced. Molten salt loops are used to transfer heat from the hydro-gasification reactor, and Fischer-Tropsch reactor if liquid fuel is produced, to the steam generator and the steam pyrolytic reformer. In another embodiment of the invention, carbonaceous material can be heated simultaneously in the presence of both hydrogen and steam to undergo steam pyrolysis and hydro-gasification in a single step.

In an image processing apparatus of the present invention, which displays image data, such as images photographed by imaging apparatus such as a digital camera and photographing conditions as images before correction and images after correction, on an image display section, distortion and color tone of images obtained from an unused camera are corrected and joined to each other as viewing images before and after correction, so that the parameters used in the correction are stored in accordance with its apparatus, and the stored parameters are read out and used to correct in images obtained from previously used camera, and the joined image and image data are displayed on a monitor and output by a printer, or stored in a recording medium.

A process and apparatus for producing a synthesis gas for use as a gaseous fuel or as feed into a Fischer-Tropsch reactor to produce a liquid fuel in a substantially self-sustaining process. A slurry of particles of carbonaceous material in water, and hydrogen from an internal source, are fed into a hydro-gasification reactor under conditions whereby methane rich producer gases are generated and fed into a steam pyrolytic reformer under conditions whereby synthesis gas comprising hydrogen and carbon monoxide are generated. A portion of the hydrogen generated by the steam pyrolytic reformer is fed through a hydrogen purification filter into the hydro-gasification reactor, the hydrogen therefrom constituting the hydrogen from an internal source. The remaining synthesis gas generated by the steam pyrolytic reformer is either used as fuel for a gaseous fueled engine to produce electricity and / or process heat or is fed into a Fischer-Tropsch or similar reactor under conditions whereby a liquid fuel is produced.

A process and apparatus for producing a synthesis gas for use as a gaseous fuel or as feed into a Fischer-Tropsch reactor to produce a liquid fuel in a substantially self-sustaining process. A slurry of particles of carbonaceous material in water, and hydrogen from an internal source, are fed into a hydro-gasification reactor under conditions whereby methane rich producer gases are generated and fed into a steam pyrolytic reformer under conditions whereby synthesis gas comprising hydrogen and carbon monoxide are generated. A portion of the hydrogen generated by the steam pyrolytic reformer is fed through a hydrogen purification filter into the hydro-gasification reactor, the hydrogen therefrom constituting the hydrogen from an internal source. The remaining synthesis gas generated by the steam pyrolytic reformer is either used as fuel for a gaseous fueled engine to produce electricity and / or process heat or is fed into a Fischer-Tropsch reactor under conditions whereby a liquid fuel is produced. Molten salt loops are used to transfer heat from the hydro-gasification reactor, and Fischer-Tropsch reactor if liquid fuel is produced, to the steam generator and the steam pyrolytic reformer.

An image synthesization method, whereby a plurality of images, each of which has a partially overlapping image area, are synthesized to create a single synthetic image, comprises a determination step of inputting a plurality of image data sets that correspond to the plurality of images that are input, and of determining whether or not an image in the partially overlapping image area of each of images that are indicated by the plurality of image data sets includes mainly characters an image processing step of performing, for the plurality of image data sets that are input, image processing in consonance with a result of a determination performed at the determination step and an image synthesization step of synthesizing images that are indicated by the resultant plurality of image data, for which the image processing has been performed at the image processing step. With this arrangement, a plurality of images can be easily and effectively synthesized.

The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desaturase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.

The present invention provides a membrane-electrode assembly which comprises: at least one rod-form or tubular electrode; a tubular diaphragm disposed around the periphery of the electrode; and a wire-form counter electrode disposed around the periphery of the diaphragm, the diaphragm being fixed to the rod-form or tubular electrode with the wire-form counter electrode to thereby form an electrode chamber having a gas / liquid passage between the diaphragm and the rod-form or tubular electrode.

A system and method of rendering overlapping layers in a computer display, such as a windowing system, employs front-to-back assembly of the displayed image. An arbitrary number of overlapping elements, such as windows, can be presented, without requiring temporary storage space or additional off-screen buffers. The front-to-back assembly technique minimizes the number of memory transfers performed in connection with rendering an image, and avoids unnecessary reading and processing of pixels that will not contribute to the final image. Special effects such as semi-transparency, shadows, and irregular shapes can be accommodated and processed in an efficient manner.

The invention discloses a virtual viewpoint synthesis method based on depth and shade information, comprising steps of: (1) defining a 3D plotting equation of a plotting method based on a depth image; (2) according to the spatial positions of the reference viewpoints and the virtual viewpoints, forming a pixel plotting sequence; (3) adopting a horizontal half pixel plotting method based on a depth image, referring to the main viewpoint, plotting an image of the virtual viewpoints; (4) according to the shade information of the auxiliary viewpoints, processing shade compensation on the virtual viewpoint image, to attain a virtual viewpoint image of high resolution; (5) converting the image of high resolution to attain an image of original resolution; (6) adopting an asymmetry linear interpolation method to process residual cavity compensation; (7) processing block effect smoothing to attain the virtual viewpoint synthesis image of high quality. The invention is suitable for parallel optical axis camera array mode, wherein the virtual viewpoints for synthesis are between the main viewpoint and the auxiliary viewpoints. Therefore, the invention can effectively synthesize virtual viewpoint images of high quality.

The present invention relates to methods of synthesizing long-chain polyunsaturated fatty acids, especially eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, in recombinant cells such as yeast or plant cells. Also provided are recombinant cells or plants which produce long-chain polyunsaturated fatty acids. Furthermore, the present invention relates to a group of new enzymes which possess desatorase or elongase activity that can be used in methods of synthesizing long-chain polyunsaturated fatty acids.

Various multiple-mask patterning methods by employing the layout decomposition and stitching technique are invented. The inventions pertain to methods of decomposing and synthesizing two-dimensional features on a substrate having the feature density increased to multiple times (up to eight times) of what is possible using the standard optical lithographic technique; and methods to release the overlay requirement when patterning the critical layers of semiconductor devices. The invented processes allow IC designers to pattern random two-dimensional circuit features that are beyond the resolution capability of optical lithography. They provide production-worthy methods for the semiconductor industry to continue IC scaling beyond the half pitch of 10 nm.

A parametric stereo upmix apparatus generates left and right signals from a mono downmix signal based on spatial parameters. The parametric stereo upmix includes a predictor configured to predict a difference signal including a difference between the left and right signals based on the mono downmix signal scaled with a prediction coefficient. The prediction coefficient is derived from the spatial parameters. The parametric stereo upmix apparatus further includes an arithmetic unit configured to derive the left and right signals based on a sum and a difference of the mono downmix signal and the difference signal.

A parametric stereo upmix apparatus (300, 400) generating a left signal (206) and a right signal (207) from a mono downmix signal (204) based on spatial parameters (205). Said parametric stereo upmix being characterized in that it comprises a means (310) for predicting a difference signal (311) comprising a difference between the left signal (206) and the right signal (207) based on the mono downmix signal (204) scaled with a prediction coefficient (321). Said prediction coefficient is derived from the spatial parameters (205). Said parametric stereo upmix apparatus (300, 400) further comprises an arithmetic means (330) for deriving the left signal (206) and the right signal (207) based on a sum and a difference of the mono downmix signal (204) and said difference signal (311).

The present disclosure identifies pathways, mechanisms, systems and methods to confer production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in engineered and / or evolved methylotrophs such that these organisms efficiently convert C1 compounds, such as formate, formic acid, formaldehyde or methanol, to organic carbon-based products of interest, and in particular the use of organisms for the commercial production of various carbon-based products of interest.

An image synthesization method, whereby a plurality of images, each of which has a partially overlapping image area, are synthesized to create a single synthetic image, comprises a determination step of inputting a plurality of image data sets that correspond to the plurality of images that are input, and of determining whether or not an image in the partially overlapping image area of each of images that are indicated by the plurality of image data sets includes mainly characters an image processing step of performing, for the plurality of image data sets that are input, image processing in consonance with a result of a determination performed at the determination step and an image synthesization step of synthesizing images that are indicated by the resultant plurality of image data, for which the image processing has been performed at the image processing step. With this arrangement, a plurality of images can be easily and effectively synthesized.

The invention provides a method of synthesizing IM-5 molecular sieve by using a composite template. The method comprises the following steps: (1) dissolving an inorganic base, an aluminum source and two templates in deionized water and carrying out uniform mixing so as to prepare a mixed solution; (2) adding a silicon source into the mixed solution obtained in step (1), optionally adding an additive and carrying out uniform mixing so as to prepare colloid or a solid-liquid mixture; (3) transferring the colloid or the solid-liquid mixture obtained in step (2) to a crystallization kettle, carrying out hydrothermal crystallization at a temperature of 120 to 200 DEG C for 1 to 10 d, carrying out cooling after crystallization and rinsing, filtering and drying the obtained mixed liquor so as to obtain raw powder of the IM-5 molecular sieve. A mole ratio of reactants satisfies the equation of SiO2: Al2O3: M2O: R1: R2: H2O = 60: 0.4-6: 6-21: 0.6-18: 0.6-12: 300-1800, wherein M2O is an alkali metal oxide, R1 is a first template, and R2 is a second template. The method provided in the invention widens the range of mixing ratios of raw materials, improves yield of a single kettle and enables time for crystallization to be further shortened and enlarged production to be easier.

An efficient synthesis for the preparation of N-[(3R,6S)-6-(2,3-difluorophenyl)-2-oxo-1-(2,2,2-trifluoroethyl)azepan-3-yl]-4-(2-oxo-2,3-dihydro-1H-imidazo[4,5-b]pyridin-1-yl)piperidine-1-carboxamide, by coupling (3R,6S)-3-amino-6-(2,3-difluorophenyl)-1-(2,2,2-trifluoroethyl)azepan-2-one and 2-oxo-1-(4-piperidinyl)-2,3-dihydro-1H-imidazo[4,5-b]pyridine dihydrochloride with 1,1′-carbonyldiimidazole (“CDI”) as carbonyl source; an efficient preparation of the potassium salt of N-[(3R,6S)-6-(2,3-difluorophenyl)-2-oxo-1-(2,2,2-trifluoroethyl)azepan-3-yl]-4-(2-oxo-2,3-dihydro-1H-imidazo[4,5-b]pyridin-1-yl)piperidine-1-carboxamide; efficient syntheses for the preparation of intermediates (3R,6S)-3-amino-6-(2,3-difluorophenyl)-1-(2,2,2-trifluoroethyl)azepan-2-one and 2-oxo-1-(4-piperidinyl)-2,3-dihydro-1H-imidazo[4,5-b]pyridine dihydrochloride, and the potassium salt of N-[(3R,6S)-6-(2,3-difluorophenyl)-2-oxo-1-(2,2,2-trifluoroethyl)azepan-3-yl]-4-(2-oxo-2,3-dihydro-1H-imidazo[4,5-b]pyridin-1-yl)piperidine-1-carboxamide including the potassium salt ethanolate and potassium salt hydrate.

A system and method of rendering overlapping layers in a computer display, such as a windowing system, employs front-to-back assembly of the displayed image. An arbitrary number of overlapping elements, such as windows, can be presented, without requiring temporary storage space or additional off-screen buffers. The front-to-back assembly technique minimizes the number of memory transfers performed in connection with rendering an image, and avoids unnecessary reading and processing of pixels that will not contribute to the final image. Special effects such as semi-transparency, shadows, and irregular shapes can be accommodated and processed in an efficient manner.

The invention discloses a catalyst for synthesis of methyl methoxyacetate (MMAC) by use of dimethoxymethane (DMM) and CO as raw materials, the catalyst is a molecular sieve with twelve membered ring cavity, ten membered ring channel and supercage structures, the International Zeolite Association (IZA) defines the topological structure of the catalyst as MWW type such as MCM-22 and MCM-49, the molecular formula is SixAlyOz, and the molecular sieve is hydrogen type. The molecular sieve SiO2 / Al2O3 ratio is 10-200. Carbonylation reaction is performed in a gas-solid phase fixed bed reactor, the reaction pressure is 1-50*105Pa, a reaction raw material gas comprises the dimethoxymethane with the partial pressure of 1-50kPa and balance of CO (1-49*105Pa) or CO / He (1-49* 105Pa), the reaction temperature is 60-180 DEG C, and the space velocity is 20-500L .g<-1>.h<-1>. Compared with a MFI type molecular sieve (HZSM-5) only having a crossed ten membered ring structure and a BEA type molecular sieve (Hbeta) only having a twelve membered ring structure, the MWW type molecular sieve has higher methyl methoxyacetate selectivity.

The invention discloses a contact network failure detection and diagnosis method based on an unmanned aerial vehicle, which comprises the following steps: (1) image acquisition: carrying a video camera to shoot along a contact network by an unmanned aerial vehicle so as to respectively acquire contact network images under visible light and infrared light; (2) image graying; (3) image enhancement; (4) image segmentation; (5) image dissection; (6) image fusion: fusing Laplacian pyramid layers under visible light with corresponding Laplacian pyramid layers under infrared light, and carrying out image reconstruction on the fused Laplacian pyramid to obtain a contact network component image after the visible light image and the infrared light image are fused; and (7) carrying out image identification and failure judgment by a BP (back-propagation) neural network. The method can be used for effectively acquiring a contact network image in the operation process of a locomotive in a multidirectional multiangular real-time mode, automatically identifying the contact network component in the image, and judging whether the contact network fails and the type of the failure; and the judgment result is more accurate and reliable, and can better ensure the safety of railway transportation.

The invention discloses a method for synthesizing methyl propionate by ethylene. The methyl propionate is prepared from the materials: ethylene, methanol, carbon monoxide and water under existence of a catalyst in a carbonyl combination manner; and the adopted catalyst is a composite catalyst system taking palladium acetate as a main catalyst, and metal ions such as cobalt, nickel or ruthenium and the like as a promoter. The method disclosed by the invention has good catalytic activity and selectivity at a low temperature and low pressure; the composite catalyst system has the advantages of low consumption, good selectivity, long service life and the like; carbonyl synthesis of the methyl propionate from the ethylene can be efficiently catalyzed; the reaction result is based on the ethylene; the yield of the methyl propionate is greater than 95%; and the entire method has good commercial value.