2207 results about "Cholesterol" patented technology

The invention provides a lipidosome, a preparation method thereof and application of the lipidosome in preparation of a medicine for treating PARP inhibitor-resistant breast cancer, and relates to the technical field of biology. The preparation method of the liposome provided by the invention comprises the following steps: under a stirring condition, injecting an ethanol solution dissolved with soybean phospholipid and cholesterol into a histidine aqueous solution for hydration, volatilizing an ethanol component after the hydration is finished, and carrying out ultrasonic treatment to prepare the liposome. The preparation method is simple and convenient, and the prepared liposome loaded with histidine is high in encapsulation efficiency. The research of the inventor finds that histidine metabolism reprogramming occurs in the PARPi resistance process of breast cancer cells, so that DNA synthesis repair is maintained. And after the PARPi-resistant breast cancer cells are treated by adopting the liposome disclosed by the invention, the histidine content in the breast cancer cells can be increased, so that the PARPi-resistant cells are sensitive to PARPi, and further the treatment on the PARPi-resistant breast cancer is realized.

The invention relates to the field of traditional Chinese medicine effective component new dosage form pharmaceutical preparations, and discloses an M2 type macrophage membrane coated ROS responsive bionic nano drug delivery system, preparation and application. Mesoporous silica is used as a core to load a traditional Chinese medicine effective component, namely tripterine, and lipid components, namely PEGylated distearoyl phosphatidyl ethanolamine (DSPE-PEG), dioleoyl lecithin (DOPC), cholesterol and the like, are used as raw materials to prepare a ROS-responsive lipid coating, so that ROS-responsive tripterine mesoporous silica is formed. And then realizing bionic fusion with the M2 type macrophage membrane on the outer layer through an ultrasonic coating technology to prepare the M2 type macrophage membrane coated ROS responsive bionic mesoporous silica nano drug delivery system. The invention can realize targeted therapy of rheumatoid arthritis, and has the advantages of biological safety and the like.

The invention relates to the technical field of health early warning, in particular to a human health early warning method and system based on physical examination data analysis, which comprises the following steps: collecting physical examination data of a human body, analyzing index changes of blood pressure, blood sugar, cholesterol and body mass index of the human body, sorting the physical examination data of the human body through time sequence sorting, and generating a time sequence sorting result. According to the method, time sequence sorting and spatial dimension evaluation are performed on the physical examination data, so that the health risk can be accurately identified and the early warning level can be dynamically evaluated, the data analysis precision is improved, the risk prediction dimension is increased, early warning is more timely and accurate, and the accuracy of early warning is improved by performing layout evaluation on the physical examination data points in a finite dimension space. Potential health abnormalities can be identified on multiple levels, and multi-dimensional analysis provides a new way for identifying complex health problems, so that health management is more active and predictable, health monitoring and risk management are optimized, and the health condition of a patient is improved.

The invention relates to a preparation method and application of an artificially synthesized exosome with an anti-aging effect, and belongs to the technical field of cosmetics and biology. The artificially synthesized exosome is prepared from hydrogenated lecithin, phytosphingosine, cholesterol, ceramide, squalane, sodium stearoyl glutamate, a surface modification structure, polyol, an active component and water. The preparation method comprises the steps of preparation of an alcohol phase, preparation of a water phase, preparation of a mixed solution and high-pressure homogenization treatment. The artificially synthesized exosome prepared by the preparation method provided by the invention is good in stability and high in anti-aging activity.

The invention provides a display screen suitable for cholesterol liquid crystal and a driving method. The display screen comprises a time schedule controller, a source driver, a grid driver and a common voltage generating circuit. The time schedule controller obtains image data and output setting, and outputs a source electrode control signal, a grid electrode control signal and a common voltage control signal; the source electrode driver outputs a source electrode driving signal according to the source electrode control signal, the source electrode driving signal comprises a multi-frame first alternating current signal and a multi-frame first direct current signal, and then output is paused until a next image is displayed; the gate driver outputs a gate driving signal according to the gate control signal; the common voltage generation circuit outputs a common voltage signal according to the common voltage control signal, and the common voltage signal comprises a multi-frame second AC signal and a multi-frame second DC signal; the second AC signal and the first AC signal are synchronous and opposite in polarity, and the second DC signal and the first DC signal are synchronous. The image display requirement can be met, and power consumption is reduced.

The invention belongs to the technical field of medicine, and discloses an active targeting atherosclerotic plaque liposome and application thereof in diagnosis, a carrier material for forming the liposome comprises targeting peptide R8 modified methoxy polyethylene glycol phospholipid (R8-DSPE-PEG), polypropylene sulfide-polyethylene glycol (PPS-PEG) and cholesterol (CH), and the targeting peptide R8 modified methoxy polyethylene glycol phospholipid (R8-DSPE-PEG), the polypropylene sulfide-polyethylene glycol (PPS-PEG) and the cholesterol (CH) are combined to form the active targeting atherosclerotic plaque liposome. The sequence of the targeting peptide R8 is Asp-Ser-Ser-Ser-Gln-Cys-His-Arg, and the sequence of the targeting peptide R8 is Asp-Ser-Ser-Gln-Cys-His- The liposome provided by the invention can improve the enrichment concentration of a fluorescent dye or a drug in local lesion, and has the advantages of low toxicity and high efficiency, and the obtained targeted liposome has a good effect on diagnosis of atherosclerosis.

The invention provides a nano photosensitizer modified by outer membrane vesicles of genetically engineered bacteria as well as a preparation method and application of the nano photosensitizer. The nano photosensitizer is a nano material with the particle size of 80-120 nm, and the nano photosensitizer modified by the genetically engineered bacterium outer membrane vesicle comprises dipalmitic acid phosphatidylcholine (DPPC), distearoyl phosphatidylcholine (DSPC), dioleoyl lecithin (DOPC), cholesterol, a small molecule dye and CD47nb-OMV membrane protein. The chemical structure of the small molecule dye is shown as a formula I or a formula II. The nano photosensitizer OBDlp modified by outer membrane vesicles of genetically engineered bacteria serves as a novel nano photosensitizer, tumor targeting PTT is mediated through NIR II FL / PA imaging, tumor immunogenicity is improved, antigen presenting cells are activated, and infiltration and functionalization of CD8 + T cells are caused. And OBDlp and laser are combined to induce release of a large amount of DAMPs and trigger ICD to promote maturation of cDC1s. Meanwhile, a CD47 nano antibody carried by the nano photosensitizer induces polarization of M1 phenotype macrophages by blocking a CD47-SIRP alpha signal transduction axis, and an immunosuppression tumor microenvironment is reversed.

The invention relates to a bionic exosome for anti-inflammation and repair as well as a preparation method and application thereof, and belongs to the field of cosmetics. The bionic exosome provided by the invention comprises a membrane structure and an internal load phase, the membrane structure comprises a phospholipid bilayer, a stabilizer and a targeting ligand; the internal load phase comprises a solvent and an active component; the phospholipid bilayer is composed of phospholipid compounds; preferably, at least one of the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and the 1, 2-dioleoyl-sn-glycerol-3-phosphoethanolamine is adopted as the phospholipid compound, and at least one of the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and the 1, 2-dioleoyl-sn-glycerol-3-phosphoethanolamine is adopted as the phospholipid compound; at least one of cholesterol, phytosphingosine and sodium stearoyl glutamate is preferably adopted as a stabilizer, so that the stabilizer of the obtained bionic exosome is improved, and the bionic exosome has the advantages of high encapsulation efficiency, good encapsulation efficiency stability, simplicity in operation, excellent anti-inflammatory and repairing effects and the like.

The invention discloses pediococcus acidilactici NDF12 and application thereof, the pediococcus acidilactici NDF12 is preserved in Guangdong Microbial Culture Collection Center on January 8, 2025, is called GDMCC for short, and the preservation number is GDMCC NO: 65732. The invention further discloses a preparation method of the pediococcus acidilactici NDF12. The strain is derived from Chinese local traditional dairy products, phenotype and genotype safety evaluation proves that the strain is safe and non-toxic, has excellent fermentation characteristics and probiotic characteristics, and can be used as a leavening agent for producing yoghourt. The bacterial strain also has gastrointestinal fluid endurance capacity, adhesion capacity, bacteriostatic activity, antioxidant activity, cholesterol lowering activity and blood sugar lowering activity, and has great application prospects in the fields of foods, medicines, feeds and the like.

The invention discloses a process and a device for continuously synthesizing vitamin D3 through photocatalysis, and relates to the technical field of process on-line monitoring and intelligent control. Photochemical reaction requirements under raw materials with different concentrations are matched through multi-band illumination weight dynamic distribution; when the concentration of the 7-dehydrocholesterol fluctuates, the illumination ratio of 290nm to 305nm wavebands is adjusted in real time by a reinforcement learning algorithm, so that the conversion of a pre-vitamin D3 intermediate is more efficient, and the competitive consumption of a by-product path is reduced; the reaction retention time is further optimized through synchronous flow rate adjustment, and local excessive irradiation caused by sudden concentration increase is avoided; the environment temperature and humidity change is incorporated into a state vector, and the control module generates a compensation instruction accordingly; and if the humidity changes suddenly, pulse type flow compensation is triggered to counteract reaction efficiency attenuation. A closed-loop response mechanism reduces the dependence on physical temperature control equipment, and is especially suitable for mobile production scenes.

The invention discloses a bionic nano-composite, a preparation method thereof and application of the bionic nano-composite in preparation of a product for treating atherosclerosis, and belongs to the technical field of medicine preparation. The bionic nano compound is a metal nano enzyme Ir-TiO2 coated by an M2 macrophage cell membrane. According to the invention, the surfaces of Ir-TiO2 nanoparticles are coated with M2 macrophage membranes, so that the nanocomposite with a bionic structure is prepared. After intravenous injection, the formed bionic nanocomposite can actively target endothelial cells in atherosclerotic lesions, and release Ir-TiO2 antagonism to endothelial cells in plaques and cytoplasm of inflammatory macrophages. After the Ir-TiO2 reaches the diseased region, the Ir-TiO2 can play an enzyme role to achieve an anti-inflammatory effect, and can promote inflammatory cells such as macrophages and smooth muscle cells at the diseased region to discharge cholesterol, reduce lipid deposition in the cells, reduce the generation of foam cells and promote the growth of plaques. The nanotherapy can effectively prevent the progress of atherosclerosis lesion inflammation and relieve atherosclerosis.

Provided is a lipid formulation which is substantially free from cholesterol and which is mixed with nucleic acid to form lipid nucleic acid nanoparticles which are capable of transfecting cells of the immune system in a less destructive manner than electroporation.

The invention relates to the technical field of organic supramolecular chemical materials, in particular to an octyloxy-modified imine gelator, bi-component gel of the octyloxy-modified imine gelator and a preparation method and application of the octyloxy-modified imine gelator. The gelator is a symmetric o-octyloxy modified p-phenylenedialdehyde-maleonitrile imine gelator, and has an obvious aggregation-induced emission property; the gelator and the cholesteryl half-ester gel can form double-component organic gel without fluorescence emission in an organic solvent; the fluorescence-emission-free double-component gel formed in tert-butyl alcohol is red fluorescence-emission gel when meeting water, and is converted into blue fluorescence-emission gel when meeting NaClO, so that water and NaClO can be rapidly and intuitively detected; the gelator has fluorescence enhancement and blue shift after encountering NaClO in a solution state, has fluorescence'off-on 'detection on NaClO, has fluorescence emission intensity reduced after Hg < 2 + > is added in the solution state, and has fluorescence'on-off' detection performance on Hg < 2 + >.

The invention relates to the technical field of organic material detection, in particular to an imine molecular sensor with a five-detection function, two-component gel as well as a preparation method and application of the imine molecular sensor. The imine molecular sensor has three signal responses of fluorescence on-off, ultraviolet absorption and color change to Fe < 3 + >, Co < 2 + > and Ni < 2 + > in a solution state, and has ultraviolet absorption and fluorescence on-off dual-detection performance to acid and alkali changes; the imine molecular sensor and cholesteryl half ester can form two-component gel through a heating-low-temperature ultrasonic cooling method; after Fe < 3 + >, Co < 2 + >, Ni < 2 + >, NaOH and HCl are respectively added, the orange of the gel becomes milky white, reddish brown, transparent red, milky white and milky white under sunlight, and the fluorescence of the orange of the gel disappears under an ultraviolet lamp, so that the rapid and sensitive detection of Fe < 3 + >, Co < 2 + >, Ni < 2 + >, acid and alkali under two states of the imine molecular solution and the gel is realized. The imine molecular sensor provided by the invention has the advantages of mild synthesis conditions, simple preparation and the like, and creates favorable conditions for popularization and application of the imine molecular sensor.

The invention provides an mRNA (messenger ribonucleic acid) vaccine and application of the mRNA vaccine in preparation of a medicine for treating tumors. The vaccine consists of ionizable lipid, cholesterol, phospholipid, a PEG (Polyethylene Glycol) derivative and mRNA (Messenger Ribonucleic Acid) containing a gene sequence for coding norovirus S protein fusion tumor antigen or a gene sequence for coding tumor antigen fusion ferritin. According to the mRNA vaccine designed by the invention, the antigen expression quantity is increased, a nanocrystallization antigen structure is formed to promote antigen processing and presentation, strong anti-tumor cell immune response is stimulated, and a tumor local microenvironment is effectively remodeled, so that a remarkable tumor inhibition effect is achieved.

The present invention provides methods for treating hypercholesterolemia. The methods of the present invention comprise administering to patients with heterozygous familial hypercholesterolemia a pharmaceutical composition comprising a PCSK9 inhibitor. In certain embodiments, the PCSK9 inhibitor is an anti-PCSK9 antibody such as the exemplary antibody referred to herein as mAb316P. The methods of the present invention are useful for treating patients with heterozygous familial hypercholesterolemia who are not adequately controlled by maximum tolerated dose statin therapy with or without other lipid lowering therapy.

The present invention relates to antisense oligonucleotides which are complementary to conserved TDP-43 binding sites on pre-mRNA transcripts, which are capable of restoring RNA binding protein function in the processing of multiple independent mRNAs in TDP-43 depleted cells. The contiguous nucleotide sequence of the antisense oligonucleotides comprise 2′-O-methoxyethyl-RNA (2′-MOE) nucleosides and the antisense oligonucleotides are attached to cholesterol moieties.

The invention discloses a brain-targeted active oxygen responsive hydrogen sulfide donor liposome, a preparation method and an application, and belongs to the technical field of pharmaceutical preparations, the structure of the brain-targeted active oxygen responsive hydrogen sulfide donor liposome comprises a lipid material and a hydrogen sulfide donor encapsulated in the lipid material, the lipid material comprises phospholipid, cholesterol, active oxygen sensitive lipid and brain-targeted lipid, and the lipid material comprises phospholipid, cholesterol, active oxygen sensitive lipid and brain-targeted lipid. The active oxygen sensitive lipid comprises a phospholipid lipophilic part, a polyethylene glycol hydrophilic part and an active oxygen sensitive bond, and the brain-targeted lipid comprises a phospholipid lipophilic part, a polyethylene glycol hydrophilic part and brain-targeted peptide. The hydrogen sulfide donor lipidosome can penetrate through a blood brain barrier, target neuronal cells, respond to an active oxygen environment of a focus part and sensitively release a hydrogen sulfide donor, so that hydrogen sulfide is distributed in the focus neuronal cells, active oxygen and active nitrogen substances are effectively removed, oxidation and nitration stress are inhibited, and damage and death of the neuronal cells are reduced; the growth and functional recovery of neuronal cells are promoted, and the application prospect in the aspect of treating central nervous system injury diseases is good.

The invention discloses a compound composition for improving sleep disorders, which is characterized by comprising the following components: an inhibitory neurotransmitter component, melatonin and a nano-lipid carrier, the nano-lipid carrier comprises phospholipid and cholesterol, the weight ratio of phospholipid to cholesterol is (1-6): 1, the weight ratio of phospholipid to cholesterol is (1-6): 1, and the weight ratio of melatonin to cholesterol is (1-6): 1. The inhibitory neurotransmitter component is wrapped in the nano-lipid carrier to form lipid nanoparticles, and the weight ratio of the inhibitory neurotransmitter component to the nano-lipid carrier is 1: (1-15). The compound composition can be prepared into a compound oral mucosa preparation, can be rapidly disintegrated when encountering saliva in the oral cavity, is good in patient compliance and small in side effect, all the components play a synergistic effect from different mechanisms through multiple target points, and the compound oral mucosa preparation can improve the sleep quality, prolong the sleep time and shorten the sleep latency; and patients with sleep disorders are helped to improve insomnia from the pathologic root.

The invention provides an AS01 derivative adjuvant, a preparation method thereof and application of the AS01 derivative adjuvant in preparation of vaccines or anti-tumor drugs, and belongs to the technical field of biological drugs or products. The AS01 derivative adjuvant is prepared from the following components in parts by mass: 0.5 to 1.0 part of cholesterol, 2.0 to 4.0 parts of dioleoyl lecithin and at least one of the following components in parts by mass: 0.1 to 0.2 part of monophosphoryl lipid A, 0.1 to 0.2 part of QS-21, 0.36 to 0.72 part of 3M-052, 0.4 to 0.8 part of muramyl dipeptide and 0.2 to 0.4 part of oligodeoxynucleotide. According to the AS01 derived adjuvant, a novel synergistic adjuvant system is developed by integrating various immunomodulators, so that the immunogenicity of a vaccine is enhanced. The HPV vaccine is prepared on the basis of the AS01 derived adjuvant, so that the immunogenicity can be enhanced, and good anti-tumor activity can be achieved. Therefore, the invention provides a new strategy for immunotherapy of HPV-related tumors.

The invention relates to a nano-liposome preparation, which is characterized in that a liposome prepared from distearoyl phosphatidyl glycerol (DSPG), cholesterol and Biotin-PEG3000-cholesterol is used as a carrier, the surface of the nano-liposome preparation is modified with an ICAM-1 monoclonal antibody, and the nano-liposome preparation is loaded with an AIE photosensitizer and Nicodil. The nano-liposome prepared by the invention has targeting and responsive drug delivery to infected lesions, the enrichment of drugs at bacterial infected parts is improved, nano-system components can be cut by phospholipase in an infected microenvironment, responsive release of the drugs at the bacterial infected parts can be realized, and the treatment effect is improved. According to the invention, a hydrophobic shell layer and a hydrophilic core of the liposome are utilized, and hydrophobic NO donor molecules and a hydrophilic AIE photosensitizer are loaded at the same time, so that synergistic treatment of bacterial infection by a photodynamic therapy and a gas therapy is realized, and a better killing effect on drug-resistant bacteria is achieved.

The invention discloses a brassica rapa imago feed and a preparation method thereof, and belongs to the technical field of insect breeding. The Eye spot Brassica rapa adult feed comprises 11 to 15 parts of casein, 1 to 3 parts of Webster salt, 4 to 6 parts of cane sugar, 9 to 12 parts of cellulose powder, 0.2 to 0.4 part of L-cysteine, 0.2 to 0.3 part of choline chloride, 0.08 to 0.1 part of inositol, 0.325 to 0.4 part of composite vitamin, 2 to 3 parts of a nutritional ingredient ethanol solution, 12 to 14 parts of a nutritional ingredient aqueous solution, 1 to 2 parts of honey, 2 to 3 parts of agar powder, 0.03 to 0.04 part of cholesterol, 33 to 67 parts of apple jam, 0.3 to 4 parts of royal jelly and 80 to 120 parts of water. In addition, the invention also provides a preparation method of the feed for the Eye spot Brassica rapa imagoes. The feed for the Eye spot Brassica rapa adults can prolong the life of the adults and improve the egg laying amount at the same time.

The invention discloses a synthesis method of 7-dehydrocholesterol and derivatives thereof, which comprises the following steps: cooling a compound (I), cuprous bromide and dimethyl sulfide compound under the protection of inert gas to obtain a reaction system, adding a Grignard reagent (II) or a Grignard reagent (III), stirring, reacting at room temperature, carrying out heating reaction, and cooling to obtain the 7-dehydrocholesterol. Performing post-treatment to obtain a product; if the product is the Grignard reagent (II), the product is 7-dehydrocholesterol and the derivative thereof; if the reagent is the Grignard reagent (III), the product is 25-hydroxy-7-dehydrocholesterol and the derivative thereof. The method is high in total yield, mild in reaction condition, simple in process route, high in product purity, low in three-waste generation amount, simple in post-treatment, environment-friendly and low in equipment requirement, the purity of 95% or above can be obtained through simple recrystallization, and conventional chemical equipment can be used for production.

The invention relates to a pharmaceutical composition. The pharmaceutical composition comprises a PCSK9 inhibitor and a GLP-1 receptor agonist, the invention also relates to application of the medicine composition in preparation of medicines for treating hyperglycemia, diabetes, obesity and / or cholesterol-related diseases.

The invention discloses a hair follicle targeting nano-liposome anti-hair loss and hair growth compound preparation. The compound preparation is prepared from the following raw materials in parts by mass: 70 to 85 parts of bionic liposome carrier and 15 to 30 parts of active ingredients, the bionic liposome carrier is prepared from 50 to 60 parts of egg yolk lecithin, 15 to 20 parts of pH sensitive phospholipid DOPE, 10 to 15 parts of cholesterol and 5 to 10 parts of mesenchymal stem cell exosome membrane protein; the active ingredients comprise 5 to 10 parts of minoxidil, 3 to 5 parts of caffeine, 0.5 to 1 part of miR-218-5p, 1 to 2 parts of biotin and 2 to 3 parts of quercetin; the average particle size of the nano-liposome formed by the bionic liposome carrier is 120-180nm, and the polydispersity index (PDI) is less than or equal to 0.15. The invention relates to the technical field of biological medicines, in particular to a hair follicle-targeted nano-liposome anti-hair loss and hair growth compound preparation, which has the following advantages due to the adoption of the technical scheme: accurate targeted delivery is realized, and the treatment safety and accuracy are improved; intelligent response release is achieved, and the local microenvironment of hair follicles is optimized; the multi-target synergistic effect breaks through the limitation of single treatment.

The invention relates to the technical field of screening and application of probiotics, and particularly provides a novel bacillus coagulans strain and application of the novel bacillus coagulans strain. The bacillus coagulans is screened from naturally fermented soybean paste, has good biological safety, strong stress resistance to gastrointestinal tract environment, high yield of exopolysaccharide and protease, strong bacteriostasis and oxidation resistance, can efficiently degrade cholesterol, is beneficial to maintaining intestinal health, and can be widely applied to the fields of food, health care products and the like.

The invention discloses a preparation method and application of a QS-21-polyamino acid nano vaccine, and belongs to the field of biomedical nano materials. The preparation method comprises the following steps: modifying gamma-PGA with phenylalanine ethyl ester (F) to obtain a polymer PF40 of which the F grafting rate is 40%; aiming at the characteristic that QS-21 is strongly combined with cholesterol, PF40 (PFC) grafted with cholesterol (C) is developed, and PFC wraps OVA and QS-21 through hydrophobic force to construct a nano vaccine OQPFC. The cholesterol on the PFC can reduce the combination of the QS-21 and the cholesterol on a cell membrane so as to reduce the biotoxicity of the QS-21, and the QS-21 loading efficiency of the nano vaccine is improved, so that a better treatment effect is achieved. The OQPFC can escape from lysosome through carboxyl protonation, and a large amount of QS-21 and OVA are released in cytoplasm, so that the synergistic cellular immune activation effect of the carrier adjuvant PFC and the saponin adjuvant QS-21 is realized.

The invention belongs to the field of active peptides, and particularly relates to application of selenium-rich polypeptide in inhibiting NLRP3 inflammasome-related inflammations and regulating cholesterol transport protection lipid metabolism abnormality, the selenium-rich polypeptide is prepared by chelating Adi-Apo fusion protein as shown in SEQ ID NO: 3 and sodium selenite (Na2SeO3); the selenium peptide chelate has the effects of remarkably reducing the concentration of TC / TG in serum, reducing the liver lipid deposition and inflammation level and reducing the atherosclerotic plaque area proportion through high oxidation resistance and high anti-inflammatory activity, and can be used for treating and / or preventing lipid metabolism disorder diseases such as metabolism-related fatty liver diseases and coronary atherosclerosis.

The invention discloses a preparation method of a vitamin C liposome preparation capable of improving encapsulation efficiency, the vitamin C liposome preparation is prepared by an ethanol injection-high pressure microjet circulation homogenization method, the homogenized liposome is small in particle size and uniform in distribution, and meanwhile, by combining a dialysis purification and buffer agent pH regulation process, the encapsulation efficiency of the vitamin C liposome preparation is improved. The prepared vitamin C liposome preparation can still keep high stability under the condition that cholesterol is not contained, and the problem that in the prior art, a vitamin C liposome preparation is low in encapsulation efficiency is effectively solved.

The invention belongs to the technical field of liquid crystal display, and discloses a multi-gray-scale cholesteric liquid crystal display device and a driving method thereof, according to the multi-gray-scale cholesteric liquid crystal display device and the driving method thereof, cholesterol liquid crystal of a pixel unit is controlled to be in a P state, an FC state and an intermediate stable state between the P state and the FC state through driving voltage, and the liquid crystal display effect is improved. And gray scale display of the pixel unit is realized. The driving time sequence of the bistable cholesterol liquid crystal is optimized, the proper driving voltage is selected, the cholesterol liquid crystal is controlled to be in an intermediate stable state through the driving voltage, and the display state of the cholesterol liquid crystal between the full-transmission state and the full-reflection state is increased, so that the gray scale number of the cholesteric phase display device is greatly increased on the premise that the number of sub-pixels is not increased, and the display effect of the cholesteric phase display device is improved. The display effect is improved.