241 results about "Interstitial fluid" patented technology

A sensor system for optical detection of multiple analytes is discussed. An example system is presented that includes a plurality of lenses configured to receive individual light signals from analyte-sensing chromophores disposed on an array when the analyte-sensing chromophores are illuminated by light while being exposed to different specific analytes within an interstitial fluid. The system further includes at least one volume phase grating (VPG) communicatively coupled to at least one of the plurality of lenses and a detector communicatively coupled to the at least one VPG so that light signals from the analyte-sensing chromophores are focused by the plurality of lenses to the at least one VPG and transmitted to the detector. The detector being operable to generate spectra with each spectrum corresponding to an individual light signal from a single analyte-sensing chromophore.

The invention relates to the technical field of biomedical sensing and health monitoring, and discloses a noninvasive glucose detection system. The system comprises an interstitial fluid extraction device, a photoelectric sensing module and a main control circuit which are integrated on a flexible substrate. The interstitial fluid extraction device drives subcutaneous interstitial fluid to migrate to the hydrogel interface layer under the action of reverse ion electroosmosis; the photoelectric sensing module receives interstitial fluid through a hydrogel interface layer, a working electrode of the photoelectric sensing module comprises a Cu monatomic imprinting recognition layer, and imprinting holes formed by Cu monatomic sites are specifically coordinated with glucose molecules. The master control circuit controls sampling and determines the glucose concentration based on the photocurrent signal. Noninvasive sampling and monatomic imprinting photoelectric detection are fused, sampling-detection integration is achieved, and the device has the advantages of being noninvasive, painless, high in selectivity, high in interference resistance, comfortable to wear and the like and is suitable for continuous monitoring of blood glucose.

Systems, methods, and devices feature a wearable device that includes a set of needles sized for access to interstitial fluid. The wearable device also includes a chemical indicator associated with each needle. A concentration of an analyte within the interstitial fluid can be determined based on one or more optical properties of the chemical indicator.

The invention discloses a method and system for reducing data errors of a dynamic glucometer, and the method comprises the steps: collecting a current signal of fluid between subcutaneous tissues in real time through an implantable sensor, and synchronously recording the working environment parameters of the sensor; carrying out denoising and normalization processing on the current signal, and carrying out temperature compensation and drift correction on the signal according to a working environment parameter; extracting time sequence features and frequency domain features from the signals processed in the step S2; training a machine learning model based on the time sequence feature data and the frequency domain feature data, wherein the machine learning model is used for predicting the blood glucose concentration and identifying abnormal data points; correcting a model prediction result through residual analysis and weighted adjustment, and performing secondary correction on abnormal data points in combination with event data input by a patient; and outputting the optimized blood glucose data to a terminal display device in a time sequence curve form. Through the process, the influence of abnormal state data on blood glucose monitoring is reduced, and the data stability is improved.

The invention provides an electrochemical luminescence MeHA microneedle patch and a preparation method and application thereof.The preparation method comprises the steps that hyaluronic acid is modified into methacrylated hyaluronic acid, MeHA solid powder is obtained after purification, and the MeHA solid powder is mixed with a photoinitiator to form a precursor solution; and pouring into a microneedle mold, centrifuging, drying and carrying out UV crosslinking to obtain the MeHA microneedle patch loaded with ABEI and glucose oxidase. Through the MeHA microneedle patch-SPE electrode integrated design, the integrated operation of skin interstitial fluid in-situ extraction-enzymatic reaction-electrochemical luminescence detection is realized, sample transfer is not needed, the interference of intermediate links is greatly reduced, the detection accuracy and efficiency are remarkably improved, and the device and the method are particularly suitable for clinical real-time monitoring scenes.

The utility model relates to the technical field of medical instruments, in particular to a pair of anti-slip rotatable bent three-blade forceps with a suction function for laparoscopic surgery, which comprises a forceps seat, blades, an opening mechanism, a forceps tube, a rotating mechanism, an inner tube, a rotating wheel, a pull rod, a pull wire, a handle and a suction tube, the blade is connected with the clamp seat through an opening mechanism, a rotating opening is formed in the head end of the clamp pipe, the clamp seat is installed on the rotating opening, the inner pipe is located in a pipe cavity of the clamp pipe, a rotating hole is formed in the tail end of the clamp pipe, the inner pipe penetrates through the rotating hole, the head end of the inner pipe is connected with the clamp seat through a rotating mechanism, and the rotating axis of the clamp seat is perpendicular to the rotating axis of the inner pipe. The utility model has the advantages that the front end of the blade is provided with the arc-shaped hook claw, so that deep pelvic tissues can be firmly grasped, and slipping can be effectively avoided; the rotating wheel is operated to adjust the angle of the clamp seat at the rotating opening through the inner pipe and the rotating mechanism, so that the traction effect of the blades is improved; a special suction channel is arranged in the forceps tube and used for sucking smoke, blood and interstitial fluid generated in the operation process.

The invention provides a GelMA microneedle patch loaded with chromogenic gold nano bipyramids and a preparation method thereof.The method comprises the steps that citric acid, chloroauric acid and a CTAC solution are mixed, then sodium borohydride is added, and a seed solution is obtained through stirring and standing; mixing chloroauric acid, silver nitrate, hydrochloric acid and CTAB (cetyltrimethyl ammonium bromide), and sequentially adding ascorbic acid and the seed solution to obtain a growth solution; carrying out centrifugation, morphology regulation and chemical etching purification to obtain a chromogenic gold nano bipyramid solution; the preparation method comprises the following steps: reacting gelatin with methacrylic anhydride in PBS, dialyzing and freeze-drying to obtain GelMA; dissolving GelMA in the gold nanoparticle bipyramid solution, and adding a photoinitiator LAP to obtain a mixed precursor solution; and the GelMA microneedle patch loaded with the gold nano bipyramid is prepared through mold casting, centrifugation, ultraviolet light crosslinking and drying demolding. The microneedle extraction and gold nano bipyramid color development integrated scheme is initiated, blood sampling is not needed, the dermis interstitial fluid is extracted in a minimally invasive mode through the microneedle, detection pain and trauma are reduced, the cross infection risk of blood treatment is avoided, and the microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle

The invention discloses a high-strength microneedle with directional holes for biological analysis sampling and a preparation method of the high-strength microneedle, and belongs to the technical field of microneedles, the method comprises the following steps: filling a silk fibroin solution into a microneedle mold to form a microneedle preform, the microneedle preform comprising a needle body part and a backing part; performing directional freezing on the microneedle preform to enable ice crystals to directionally grow in the direction from the backing part to the needle body part, and demolding to obtain a frozen blank; the frozen blank is placed in an organic solution of a cross-linking agent, soaked at the low temperature lower than 0 DEG C for ice template low-temperature solvent exchange-cross-linking treatment, taken out and cleaned, then soaked in a salt solution for salting-out treatment strengthening, taken out and cleaned and freeze-dried, and the high-strength microneedle with the directional holes is obtained. The microneedle prepared through the method has high strength and excellent puncture performance, and the skin interstitial fluid collection speed and efficiency are greatly improved.

The invention discloses a self-driven diagnosis and treatment integrated microneedle array paper-based chip as well as a preparation method and application thereof, and belongs to the technical field of biomedicine. The microneedle array chip system comprises a function integration module which at least comprises a self-powered extraction unit, a sensing detection unit and a controlled drug delivery unit. According to the microneedle array paper-based chip, biomarkers in skin interstitial fluid are efficiently collected based on a self-powered thermoelectric chemical battery microneedle, PtPB (at) PEG (at) HA nanoparticles loaded on an immunochromatography test strip combination pad are triggered to be subjected to ring opening, a hybridization chain reaction is induced to generate DNA nano-enzyme, and TMB is catalyzed to develop color; the open-loop nanoparticles can be used for SERS cross detection of biomarkers, and can be used as a thermal initiator to activate the phase change microneedle under the irradiation of near-infrared light, so that drugs are released as required, and precise treatment is realized. Besides, objective quantitative analysis of color signals is realized by constructing a convolutional neural network model, and the system has the functions of naked eye screening, intelligent quantification and personalized treatment, and can improve the home management ability of early skin cancer.

The invention discloses a hydrogel microneedle patch for noninvasive blood glucose detection as well as a preparation method and application of the hydrogel microneedle patch. According to the invention, the microneedle patch is prepared by a turnover technology and is noninvasively applied to the skin surface, so that simple and rapid extraction of interstitial fluid in vivo and a new method for real-time, rapid and quantitative detection of blood glucose in vivo are realized. The hydrogel microneedle patch contains two hydrogel components: one hydrogel component is hydrogel which can be cured and formed by ultraviolet light, and the other hydrogel component is hydrogel which can be cured and formed by ultraviolet light; and the other hydrogel is functional hydrogel with pH responsiveness, and glucose oxidase is added, so that the hydrogel can sensitively react to in-vivo glucose. The double-response hydrogel is jointly mixed and prepared into a microneedle patch form, the microneedle patch is endowed with noninvasive / minimally invasive property when applied to the skin surface, the microneedle patch is very suitable for extraction of interstitial fluid in vivo, and then real-time, rapid and accurate detection of the blood glucose level in vivo is achieved.

A sensor (e.g., an optical sensor) that may be implanted within a living animal (e.g., a human) and may be used to measure an analyte (e.g., glucose or oxygen) in a medium (e.g., interstitial fluid, blood, or intraperitoneal fluid) within the animal. The sensor may include a sensor housing, an analyte indicator covering at least a portion of the sensor housing, and one or more compounds containing boronate or boronic acid moieties that reduce degradation of the analyte indicator.

The invention discloses a plasma concentration detection method, and belongs to the field of biomedical detection.The plasma concentration detection method comprises the steps that a saliva sample and a mesenchymal fluid sample of a to-be-detected object are synchronously collected through non-invasive equipment and minimally invasive equipment, and after centrifugation and membrane filtration pretreatment, the saliva sample and the mesenchymal fluid sample are obtained; a multi-channel biosensor is used for detecting drug metabolite concentrations in saliva and interstitial fluid respectively, a blood drug concentration predicted value is further calculated through a composite calibration model, the model is constructed based on a random forest algorithm, saliva and interstitial fluid concentration data are input, the blood drug concentration is output, and dynamic correction is conducted through species characteristic parameters. Wherein species parameters are obtained in real time through near infrared spectrum, weight coefficients (alpha and beta) are optimized in combination with a multiple regression equation, and finally high-precision prediction is achieved through a weighting algorithm. The method has the advantages of non-invasiveness, cross-species universality, dynamic adaptive correction and the like, and is suitable for clinical monitoring, veterinary medicine and pharmacokinetic research.

The present disclosure refers to an in vivo analyte sensor, comprising: a substrate (20); a conductive layer (21) disposed over at least a portion of the substrate (20); a sensing layer (24) disposed formed on the conductive layer (21), wherein the sensin\g layer (24) forms a sensing area configured to generate one or more signals identifying a monitored analyte level after insertion of the analyte sensor and when maintained in fluid contact with interstitial fluid; and a membrane layer (25) formed at least on the sensing layer (24). The sensing layer (24) is formed with an area sensitivity of 0,2 to 1,5 nA / (mqx / dL) mm2 within the sensing area. Further, a method of manufacturing an in vivo analyte sensor is provided.

A sensor (e.g., an optical sensor) that may be implanted within a living animal (e.g., a human) and may be used to measure an analyte (e.g., glucose or oxygen) in a medium (e.g., interstitial fluid, blood, or intraperitoneal fluid) within the animal. The sensor may include a sensor housing, an analyte indicator covering at least a portion of the sensor housing, and one or more compounds having dithio-, thio- or mercapto-containing moieties that reduce degradation of the analyte indicator.

The invention provides a methylation marker for risk assessment of prostatic cancer and application of the methylation marker, and relates to the technical field of molecular biomedicine. The invention provides a methylation marker for early non-invasive identification of prostatic cancer and a combination thereof, and a new marker group related to identification of prostatic cancer is identified based on methylation sequencing data analysis of measured tissue interstitial fluid and urine EV DNA samples of prostatic cancer patients and cancer-free patients. The marker group can effectively distinguish benign and malignant prostate cancer or distinguish inert prostate cancer and highly invasive prostate cancer, a new biomarker is provided for prostate cancer identification, and compared with the previously reported and used technical means, the marker group can more sensitively detect the methylation change of related genes of prostate cancer, and has a good application prospect. More favorable evidence is provided for early diagnosis of the prostate cancer, and a new method is provided for early diagnosis of the prostate cancer.

The invention discloses a microneedle array electrochemical sensor for monitoring a sepsis marker C-reactive protein in skin interstitial fluid. According to the novel-arrangement cactus-like structure microneedle array electrode, the enrichment efficiency of detection liquid is remarkably improved through the design that the structure sequentially extends from the center to the periphery, and good attachment to skin tissue is achieved in combination with a flexible substrate material. Electrode interface construction of the electrochemical sensor is based on electrochemical deposition, a C-reactive protein aptamer is taken as a recognition object, a highly uniform nucleic acid C-reactive protein aptamer probe single layer is formed by combining a freezing-unfreezing method self-assembly technology, and the nucleic acid C-reactive protein aptamer probe single layer has a good linear relationship with a detection electric signal in a C-reactive protein concentration range of 0.5-100 ng / mL; the kit has the properties of high sensitivity, high specificity, rapid response, real-time monitoring, minimally invasive property and the like, is beneficial to rapid detection of C-reactive protein and dynamic monitoring of sepsis course change, and also provides technical support for development of biosensors for biomarker detection in complex media.

A continuous potassium measurement system for determining potassium levels in a user over time is provided. The system includes a continuous potassium sensor (CPS) positioned in interstitial fluid of the user. The system further includes programmed circuitry comprising a processor and a memory where the CPS and programmed circuitry are communicatively coupled. The programmed circuitry is programmed with logic and instructions for completion of the following steps by the programmed circuitry: (i) obtaining data related to the level of potassium in the user from the CPS; (ii) determining the level of potassium in the user from the data obtained in step (i); (iii) storing the level of potassium in the user determined in step (ii) in the memory; and (iv) repeating steps (i) to (iii) to determine and store in the memory potassium levels in a user over time.

The invention provides construction of an electrochemical biosensor based on a fucoidin hydrogel microneedle and application of the electrochemical biosensor in online monitoring of uric acid, and relates to the technical field of biosensors. According to the micro-needle, Gantrez S-97 and PEG are subjected to copolymerization crosslinking to form a hydrogel main body, fucoidin is introduced for blending modification, a three-dimensional network structure is constructed by utilizing a high-temperature esterification reaction for regulating and controlling pH, and the super-expansion hydrogel micro-needle with high swelling property and good biocompatibility is formed. The microneedle can rapidly collect a skin interstitial fluid sample, and then through a screen printing electrode (SPE) platform, carboxylated MWCNTs are used for modifying an electrode and immobilizing uricase to construct an enzyme sensor. Hydrogen peroxide generated by catalyzing uric acid on the surface of the electrode participates in electrochemical reaction, and an output signal is used for real-time detection of uric acid concentration. The device integrates minimally invasive sampling and high-sensitivity electrochemical detection, has the advantages of quick response, simplicity and convenience in operation, good biocompatibility and the like, and is suitable for application scenes of continuous monitoring of uric acid, chronic disease management and the like.

This disclosure provides a wearable electrochemical biosensor for detecting at least one target analyte in interstitial fluid of a subject, comprising, a microneedle array comprising a highly- crosslinked hydrogel polymer; at least one electrochemical chip comprising at least one transducer surface configured for generating a signal in the presence of the at least one target analyte; and a low crosslinked hydrogel polymer, wherein the low crosslinked hydrogel polymer is configured to couple the microneedle array with the at least one transducer surface of the electrochemical chip. Methods of making and use thereof are also disclosed.

The invention provides a coin-sized, fully integrated and wearable continuous blood glucose monitoring (CGM) system in combination with frontier technologies in crossing fields of biosensors, minimally invasive tools, hydrogels and the like. The invention comprises the following three main parts: 1) an emerging biochemical amplifier, namely an organic electrochemical transistor (OECT), for improving the sensitivity and exceeding the traditional electrochemical sensor; 2) a microneedle array for sampling interstitial fluid (ISF), which reduces pain in the skin penetration process; and 3) a tough and viscous enzyme hydrogel film which can improve the reliability of glucose sensing on the skin. Different from a traditional CGM, the CGM (OECT-CGM) provided by the invention has high anti-noise capability, adjustable sensitivity and current regeneration capability due to the adoption of the OECT amplifier, so that long-term stable glucose sensing can be realized within a specific clinical range (1-20mM). The CGM provided by the invention can provide high and adjustable sensitivity, minimally invasive property and better wearable property at the same time, and also paves a way for developing the next generation of CGM.

Embodiments described herein relate to an analyte monitoring device having a user interface with a display and a plurality of actuators. The display is configured to render a plurality of display screens, including a home screen and an alert screen. The home screen is divided into a plurality of simultaneously displayed panels, with a first panel displays a rate of change of continuously monitored analyte levels in interstitial fluid, a second panel simultaneously displays a current analyte level and an analyte trend indicator, and a third panel displays status information of a plurality of components of the device. When an alarm condition is detected, the display renders the alert screen in place of the home screen, the alert screen displaying information corresponding to the detected alarm condition. Furthermore, the actuators are configured to affect further output of the analyte monitoring device corresponding to the detected condition.

The invention discloses a preparation method of an SERS (Surface Enhanced Raman Scattering) sensing microneedle patch and application of the SERS sensing microneedle patch in detection of trace blood sugar, and belongs to the technical field of material synthesis and biosensing. According to the preparation method of the SERS sensing microneedle patch disclosed by the invention, a surface enhanced Raman spectroscopy technology and a microneedle patch technology are integrated, a designed SERS probe takes urchin-shaped gold nanoparticles as a core, and a typical core-shell structure is formed through layer-by-layer self-assembly of polydopamine and gradual re-reduction of the gold nanoparticles; the Raman scattering signal is obviously enhanced. The upper layer of the double-layer structure microneedle is hyaluronic acid wrapping glucose oxidase, and the lower layer of the double-layer structure microneedle is methacryloyl hyaluronic acid wrapping an SERS probe. After glucose of interstitial fluid is collected by the microneedle, Raman spectrum characteristic fingerprint change of the SERS probe is caused through catalysis of glucose oxidase, non-invasive, rapid and high-sensitivity blood glucose detection is achieved, and the method is a more comfortable and convenient self-monitoring method.

The invention provides a multicolor visual semi-quantitative detection method for miRNA in skin interstitial fluid, which comprises the following steps: extracting a skin interstitial fluid sample in a minimally invasive manner by combining a microneedle patch with a vacuum negative pressure technology; the method comprises the following steps: incubating a skin interstitial fluid sample and a magnetic nanoprobe of which the surface is immobilized with a hairpin probe H1, opening H1 in the presence of target miRNA, triggering a subsequent hybridization chain reaction of a hairpin probe H2 and H3, and constructing a biotin-rich DNA compound on the surface of a magnetic bead; the method comprises the following steps: carrying out enzyme labeling by using an alkaline phosphatase-streptavidin conjugate, catalyzing ascorbic acid phosphate to generate ascorbic acid to reduce potassium iodate, and generating an iodine elementary substance to etch a gold nano bipyramid, so that the color of a solution is changed, and high-sensitivity detection on target miRNA is realized. The multi-color visual detection method provided by the invention is combined with the gold nano bipyramid localized surface plasmon resonance property change, so that near-full spectrum color change can be generated, and the portability of the sensor and the accuracy of visual analysis can be improved.

The invention aims to solve the problems that the existing skin photoaging evaluation technology can only realize static detection, lacks environment dynamic monitoring and cannot capture day and night changes of physiological parameters. According to the system, key parameters such as skin percutaneous moisture loss, tissue impedance and temperature are continuously measured through a wearable flexible electronic patch, a portable environment monitoring module is used for measuring UVA / UVB intensity and pollutants and is linked with satellite meteorological data to construct a personal exposure map, and a non-invasive microneedle sensing technology is used for detecting skin interstitial fluid biomarkers. A time-space diagram neural network model is used for fusing data, oxidative stress, collagen degradation and environmental toxicity are comprehensively considered, a light aging index is output, clinical intervention suggestions are provided through a data analysis and prediction module, and dynamic accurate evaluation is achieved.

A continuous sodium measurement system for determining sodium levels in a user over time is provided. The system includes a continuous sodium sensor (CSS) positioned in interstitial fluid of the user and configured to determine data related to a sodium level in the user. The system also includes programmed circuitry comprising a processor and a memory communicatively coupled with the CSS. The programmed circuitry is programmed with logic and instructions for completion of the following steps by the programmed circuitry: (i) obtaining data related to the level of sodium in the user from the CSS; (ii) determining the level of sodium in the user from the data obtained in step (i); (iii) storing the level of sodium in the user determined in step (ii) in the memory; and (iv) repeating steps (i) to (iii) to determine and store in the memory sodium levels in a user over time.

The present invention belongs to the technical field related to cardiac monitoring, and discloses a passive sensor for real-time cardiac monitoring and its application. The passive sensor includes a monitoring unit, a flexible substrate, and a signal receiving unit, wherein: the monitoring unit is a thin film composed of a material with an electric double layer effect. Thus, cations and anions in the interstitial fluid around the heart are adsorbed on the surface of the monitoring unit to form an electric double layer structure. When the cardiac muscle relaxes and contracts or the blood flow signal is transmitted, the change in pressure will further cause the change in the effective charge adsorption area, resulting in the change of the current signal; the flexible substrate is used to isolate the monitoring unit and the signal receiving unit; the signal receiving unit is connected to the monitoring unit for receiving the charge change on the surface of the monitoring unit, and serves as a counter electrode to form a closed loop with the monitoring unit. This application can realize the real-time monitoring and signal acquisition of cardiac mechanical signals, with high sensitivity and high accuracy.

The invention discloses a swelling microneedle patch and a preparation method thereof. The swelling microneedle patch comprises a backing and a swelling microneedle array adhered to the backing, the swelling microneedle array comprises a plurality of microneedles; the microneedle is in the shape of a cone or a rectangular pyramid; the height of the microneedle is 100 to 1000 [mu] m; the diameter of the tip end of the microneedle is 5-30 microns; the diameter of the bottom of the microneedle is 50-500 [mu] m; and the center-to-center distance between every two adjacent microneedles is 50-2000 microns. The swelling microneedle patch provided by the invention can overcome the barrier action of cuticle to penetrate subcutaneously and absorb subcutaneous tissue interstitial fluid, and is simple in preparation process, low in cost and high in universality. The patch is combined with a colloidal gold lateral chromatography test strip, so that the limitations that in-vitro diagnosis of infectious diseases depends on a blood sample, professional medical personnel are needed for operation, the subject compliance is poor and the like can be solved, and the patch is suitable for rapid screening and diagnosis of the infectious diseases.

An assembly for promoting interstitial fluid flow can include an implant having first and second electrodes, a control unit, and one or more magnets. The implant can be disposed at a selected site of a subject, and the control unit can be electrically coupled to the first and second electrodes and can provide power to the first and second electrodes to generate an electric field at the selected site. The one or more magnets can be disposed adjacent to the selected site and can generate magnetic field in a direction orthogonal to the electric field in order to drive electrically conductive interstitial fluid flow at the selected site.

A device for intermittent monitoring of a biomolecule in a human body, and use thereof. The device includes a device casing, a microneedle array including an array of needles configured to extract interstitial fluid from a user, a microfluidic system defining a fluidic channel including a biomolecule debonding agent, wherein the fluidic channel is in fluidic communication with the microneedle array and configured to facilitate flow of the extracted interstitial fluid therethrough, and an assay system in fluidic communication with the microfluidic system. The assay system includes an affinity binding agent capable of generating an indication of a level of one or more biomolecules present in the extracted interstitial fluid. The microneedle array, the microfluidic system, and assay system are disposed within the device casing.

Provided in the present application are a blood glucose monitoring method and a related apparatus. In the method, the blood glucose level at a first moment can be acquired on the basis of a glucose concentration in interstitial fluid at the first moment, a glucose concentration in the interstitial fluid within a time period before the first moment and a glucose concentration in the interstitial fluid within a time period after the first moment. In the present application, the glucose concentration in the interstitial fluid within the time period after the first moment is taken into consideration, and an additional time period is provided for the diffusion of glucose between the interstitial fluid and blood; therefore, when the blood glucose level drastically changes, the glucose concentration in the interstitial fluid more closely approximates the blood glucose level, and thus the accuracy of blood glucose monitoring can be improved.