117 results about "Interstitial fluid" patented technology

The invention relates to the technical field of biomedical sensing and health monitoring, and discloses a noninvasive glucose detection system. The system comprises an interstitial fluid extraction device, a photoelectric sensing module and a main control circuit which are integrated on a flexible substrate. The interstitial fluid extraction device drives subcutaneous interstitial fluid to migrate to the hydrogel interface layer under the action of reverse ion electroosmosis; the photoelectric sensing module receives interstitial fluid through a hydrogel interface layer, a working electrode of the photoelectric sensing module comprises a Cu monatomic imprinting recognition layer, and imprinting holes formed by Cu monatomic sites are specifically coordinated with glucose molecules. The master control circuit controls sampling and determines the glucose concentration based on the photocurrent signal. Noninvasive sampling and monatomic imprinting photoelectric detection are fused, sampling-detection integration is achieved, and the device has the advantages of being noninvasive, painless, high in selectivity, high in interference resistance, comfortable to wear and the like and is suitable for continuous monitoring of blood glucose.

The invention provides an electrochemical luminescence MeHA microneedle patch and a preparation method and application thereof.The preparation method comprises the steps that hyaluronic acid is modified into methacrylated hyaluronic acid, MeHA solid powder is obtained after purification, and the MeHA solid powder is mixed with a photoinitiator to form a precursor solution; and pouring into a microneedle mold, centrifuging, drying and carrying out UV crosslinking to obtain the MeHA microneedle patch loaded with ABEI and glucose oxidase. Through the MeHA microneedle patch-SPE electrode integrated design, the integrated operation of skin interstitial fluid in-situ extraction-enzymatic reaction-electrochemical luminescence detection is realized, sample transfer is not needed, the interference of intermediate links is greatly reduced, the detection accuracy and efficiency are remarkably improved, and the device and the method are particularly suitable for clinical real-time monitoring scenes.

The invention provides a GelMA microneedle patch loaded with chromogenic gold nano bipyramids and a preparation method thereof.The method comprises the steps that citric acid, chloroauric acid and a CTAC solution are mixed, then sodium borohydride is added, and a seed solution is obtained through stirring and standing; mixing chloroauric acid, silver nitrate, hydrochloric acid and CTAB (cetyltrimethyl ammonium bromide), and sequentially adding ascorbic acid and the seed solution to obtain a growth solution; carrying out centrifugation, morphology regulation and chemical etching purification to obtain a chromogenic gold nano bipyramid solution; the preparation method comprises the following steps: reacting gelatin with methacrylic anhydride in PBS, dialyzing and freeze-drying to obtain GelMA; dissolving GelMA in the gold nanoparticle bipyramid solution, and adding a photoinitiator LAP to obtain a mixed precursor solution; and the GelMA microneedle patch loaded with the gold nano bipyramid is prepared through mold casting, centrifugation, ultraviolet light crosslinking and drying demolding. The microneedle extraction and gold nano bipyramid color development integrated scheme is initiated, blood sampling is not needed, the dermis interstitial fluid is extracted in a minimally invasive mode through the microneedle, detection pain and trauma are reduced, the cross infection risk of blood treatment is avoided, and the microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle microneedle

The invention discloses a high-strength microneedle with directional holes for biological analysis sampling and a preparation method of the high-strength microneedle, and belongs to the technical field of microneedles, the method comprises the following steps: filling a silk fibroin solution into a microneedle mold to form a microneedle preform, the microneedle preform comprising a needle body part and a backing part; performing directional freezing on the microneedle preform to enable ice crystals to directionally grow in the direction from the backing part to the needle body part, and demolding to obtain a frozen blank; the frozen blank is placed in an organic solution of a cross-linking agent, soaked at the low temperature lower than 0 DEG C for ice template low-temperature solvent exchange-cross-linking treatment, taken out and cleaned, then soaked in a salt solution for salting-out treatment strengthening, taken out and cleaned and freeze-dried, and the high-strength microneedle with the directional holes is obtained. The microneedle prepared through the method has high strength and excellent puncture performance, and the skin interstitial fluid collection speed and efficiency are greatly improved.

The invention discloses a self-driven diagnosis and treatment integrated microneedle array paper-based chip as well as a preparation method and application thereof, and belongs to the technical field of biomedicine. The microneedle array chip system comprises a function integration module which at least comprises a self-powered extraction unit, a sensing detection unit and a controlled drug delivery unit. According to the microneedle array paper-based chip, biomarkers in skin interstitial fluid are efficiently collected based on a self-powered thermoelectric chemical battery microneedle, PtPB (at) PEG (at) HA nanoparticles loaded on an immunochromatography test strip combination pad are triggered to be subjected to ring opening, a hybridization chain reaction is induced to generate DNA nano-enzyme, and TMB is catalyzed to develop color; the open-loop nanoparticles can be used for SERS cross detection of biomarkers, and can be used as a thermal initiator to activate the phase change microneedle under the irradiation of near-infrared light, so that drugs are released as required, and precise treatment is realized. Besides, objective quantitative analysis of color signals is realized by constructing a convolutional neural network model, and the system has the functions of naked eye screening, intelligent quantification and personalized treatment, and can improve the home management ability of early skin cancer.

The present disclosure refers to an in vivo analyte sensor, comprising: a substrate (20); a conductive layer (21) disposed over at least a portion of the substrate (20); a sensing layer (24) disposed formed on the conductive layer (21), wherein the sensin\g layer (24) forms a sensing area configured to generate one or more signals identifying a monitored analyte level after insertion of the analyte sensor and when maintained in fluid contact with interstitial fluid; and a membrane layer (25) formed at least on the sensing layer (24). The sensing layer (24) is formed with an area sensitivity of 0,2 to 1,5 nA / (mqx / dL) mm2 within the sensing area. Further, a method of manufacturing an in vivo analyte sensor is provided.

The invention provides a methylation marker for risk assessment of prostatic cancer and application of the methylation marker, and relates to the technical field of molecular biomedicine. The invention provides a methylation marker for early non-invasive identification of prostatic cancer and a combination thereof, and a new marker group related to identification of prostatic cancer is identified based on methylation sequencing data analysis of measured tissue interstitial fluid and urine EV DNA samples of prostatic cancer patients and cancer-free patients. The marker group can effectively distinguish benign and malignant prostate cancer or distinguish inert prostate cancer and highly invasive prostate cancer, a new biomarker is provided for prostate cancer identification, and compared with the previously reported and used technical means, the marker group can more sensitively detect the methylation change of related genes of prostate cancer, and has a good application prospect. More favorable evidence is provided for early diagnosis of the prostate cancer, and a new method is provided for early diagnosis of the prostate cancer.

The invention provides a multicolor visual semi-quantitative detection method for miRNA in skin interstitial fluid, which comprises the following steps: extracting a skin interstitial fluid sample in a minimally invasive manner by combining a microneedle patch with a vacuum negative pressure technology; the method comprises the following steps: incubating a skin interstitial fluid sample and a magnetic nanoprobe of which the surface is immobilized with a hairpin probe H1, opening H1 in the presence of target miRNA, triggering a subsequent hybridization chain reaction of a hairpin probe H2 and H3, and constructing a biotin-rich DNA compound on the surface of a magnetic bead; the method comprises the following steps: carrying out enzyme labeling by using an alkaline phosphatase-streptavidin conjugate, catalyzing ascorbic acid phosphate to generate ascorbic acid to reduce potassium iodate, and generating an iodine elementary substance to etch a gold nano bipyramid, so that the color of a solution is changed, and high-sensitivity detection on target miRNA is realized. The multi-color visual detection method provided by the invention is combined with the gold nano bipyramid localized surface plasmon resonance property change, so that near-full spectrum color change can be generated, and the portability of the sensor and the accuracy of visual analysis can be improved.

The invention aims to solve the problems that the existing skin photoaging evaluation technology can only realize static detection, lacks environment dynamic monitoring and cannot capture day and night changes of physiological parameters. According to the system, key parameters such as skin percutaneous moisture loss, tissue impedance and temperature are continuously measured through a wearable flexible electronic patch, a portable environment monitoring module is used for measuring UVA / UVB intensity and pollutants and is linked with satellite meteorological data to construct a personal exposure map, and a non-invasive microneedle sensing technology is used for detecting skin interstitial fluid biomarkers. A time-space diagram neural network model is used for fusing data, oxidative stress, collagen degradation and environmental toxicity are comprehensively considered, a light aging index is output, clinical intervention suggestions are provided through a data analysis and prediction module, and dynamic accurate evaluation is achieved.

A continuous sodium measurement system for determining sodium levels in a user over time is provided. The system includes a continuous sodium sensor (CSS) positioned in interstitial fluid of the user and configured to determine data related to a sodium level in the user. The system also includes programmed circuitry comprising a processor and a memory communicatively coupled with the CSS. The programmed circuitry is programmed with logic and instructions for completion of the following steps by the programmed circuitry: (i) obtaining data related to the level of sodium in the user from the CSS; (ii) determining the level of sodium in the user from the data obtained in step (i); (iii) storing the level of sodium in the user determined in step (ii) in the memory; and (iv) repeating steps (i) to (iii) to determine and store in the memory sodium levels in a user over time.

The invention discloses a swelling microneedle patch and a preparation method thereof. The swelling microneedle patch comprises a backing and a swelling microneedle array adhered to the backing, the swelling microneedle array comprises a plurality of microneedles; the microneedle is in the shape of a cone or a rectangular pyramid; the height of the microneedle is 100 to 1000 [mu] m; the diameter of the tip end of the microneedle is 5-30 microns; the diameter of the bottom of the microneedle is 50-500 [mu] m; and the center-to-center distance between every two adjacent microneedles is 50-2000 microns. The swelling microneedle patch provided by the invention can overcome the barrier action of cuticle to penetrate subcutaneously and absorb subcutaneous tissue interstitial fluid, and is simple in preparation process, low in cost and high in universality. The patch is combined with a colloidal gold lateral chromatography test strip, so that the limitations that in-vitro diagnosis of infectious diseases depends on a blood sample, professional medical personnel are needed for operation, the subject compliance is poor and the like can be solved, and the patch is suitable for rapid screening and diagnosis of the infectious diseases.

A device for intermittent monitoring of a biomolecule in a human body, and use thereof. The device includes a device casing, a microneedle array including an array of needles configured to extract interstitial fluid from a user, a microfluidic system defining a fluidic channel including a biomolecule debonding agent, wherein the fluidic channel is in fluidic communication with the microneedle array and configured to facilitate flow of the extracted interstitial fluid therethrough, and an assay system in fluidic communication with the microfluidic system. The assay system includes an affinity binding agent capable of generating an indication of a level of one or more biomolecules present in the extracted interstitial fluid. The microneedle array, the microfluidic system, and assay system are disposed within the device casing.

Provided in the present application are a blood glucose monitoring method and a related apparatus. In the method, the blood glucose level at a first moment can be acquired on the basis of a glucose concentration in interstitial fluid at the first moment, a glucose concentration in the interstitial fluid within a time period before the first moment and a glucose concentration in the interstitial fluid within a time period after the first moment. In the present application, the glucose concentration in the interstitial fluid within the time period after the first moment is taken into consideration, and an additional time period is provided for the diffusion of glucose between the interstitial fluid and blood; therefore, when the blood glucose level drastically changes, the glucose concentration in the interstitial fluid more closely approximates the blood glucose level, and thus the accuracy of blood glucose monitoring can be improved.

The application provides a skin interstitial fluid miRNA multicolor visualization semi-quantitative detection method, which comprises the following steps: incubating a skin interstitial fluid sample with magnetic nano probes of surface immobilized hairpin probes H1, opening H1 and triggering a subsequent hybridization chain reaction of hairpin probes H2 and H3 in the presence of target miRNA, and constructing a biotin-rich DNA complex on the surface of the magnetic beads; performing enzyme labeling by using an alkaline phosphatase-streptavidin coupling compound, and catalyzing ascorbic acid phosphate to generate ascorbic acid potassium iodate, so that the color of the solution changes, and high-sensitivity detection of the target miRNA is realized. The multicolor visualization detection method can produce near-full-spectrum color changes by combining the gold nano double-cone local surface plasmon resonance property change, and is beneficial to improving the portability of the sensor and the accuracy of the visual analysis.

A system and method for monitoring body chemistry of a user, the system comprising: a housing supporting: a microsensor comprising a first and second working electrode, a reference electrode, and a counter electrode, and configured to access interstitial fluid of the user, and an electronics subsystem comprising a signal conditioning module that receives a signal stream, from the microsensor, wherein the electronics subsystem is configured to detect an impedance signal derived from two of the first working electrode, the second working electrode, the reference electrode, and the counter electrode; and a processing subsystem comprising: a first module configured to generate an analysis indicative of an analyte parameter of the user and derived from the signal stream and the impedance signal, and a second module configured to transmit information derived from the analysis to the user, thereby facilitating monitoring of body chemistry of the user.

The invention relates to a biological self-cleaning semipermeable membrane as well as preparation and application thereof. Specifically, the invention discloses a biological self-cleaning semipermeable membrane, and the semipermeable membrane still has excellent permselectivity after being placed in tissue fluid for a long time. The invention also discloses preparation and application of the semipermeable membrane.

An implantable biomedical sensor (1) is disclosed which is characterized by a novel electrofluidic design based on dedicated measurement chambers (2, 3) enclosed by respective membranes (4, 5) which are permeable to water molecules and optionally typical ions found in human interstitial fluid (ISF). By this concept, the sensor (1) can perform electrical measurements in the respective chambers (2, 3), collecting data that allows conclusions to be drawn about the amount of water and ions present in the ISF around the sensor (1). In other words, such a sensor (1) enables electrical monitoring of the electrolyte state and / or hydration state in the tissue of a patient wearing the implantable sensor (1).

A sensor (e.g., an optical sensor) that may be implanted within a living animal (e.g., a human) and may be used to measure an analyte (e.g., glucose or oxygen) in a medium (e.g., interstitial fluid, blood, or intraperitoneal fluid) within the animal. The sensor may include a sensor housing, an analyte indicator covering at least a portion of the sensor housing, and one or more compounds containing boronate or boronic acid moieties that reduce degradation of the analyte indicator.

The present application relates to a method of treating an inflammatory skin condition by administering a pharmaceutical composition comprising a reduced dose of minocycline to a subject in need thereof, wherein said administration provides an effective plasma or interstitial fluid concentration of minocycline for treating the inflammatory skin condition.

The application belongs to the technical field of sensor preparation, and particularly relates to an integrated interstitial fluid sampling and electrochemical detection system and a preparation method and application thereof. The integrated interstitial fluid sampling and electrochemical detection system comprises a microneedle array unit, an electrochemical sensor and a micro negative pressure pump. The microneedle array unit has good biocompatibility, is safe and reliable, and will not damage blood vessels and nerves of a patient; the microneedle is short and small, has weak discomfort, does not affect normal activities of a user, and is more conducive to continuous long-term monitoring; the electrochemical sensor can accurately identify a template molecule and transmit a signal; and the micro negative pressure pump can generate vacuum pressure to collect a sample amount meeting detection. The integrated interstitial fluid sampling and electrochemical detection system can be used by being inserted into a subcutaneous or intradermal position of a human body, can realize real-time and integrated sampling-detection, and can detect various biomarkers.

A method of measuring tissue properties using a fluid line in fluid communication with a subcutaneous tissue includes applying a pressure profile or flow profile to the subcutaneous tissue via the fluid line, with the fluid line isolated from atmospheric pressure, measuring a pressure value or flow rate value over time in response to the applied pressure profile or applied flow profile, and calculating, using at least one processor, at least one of the following: time constant of pressure decay; fluidic resistance of tissue; permeability of tissue; mechanical compliance of tissue; osmotic pressure of interstitial fluid of tissue; capillary pressure in subcutaneous tissue; and ionic strength of interstitial fluid.

The present invention relates to methods of assessing heart failure (HF), determining the risk of developing HF, monitoring HF, classifying HF, risk stratification and prognosis in patients with HF and determining the therapeutic effect of a treatment regimen for HF in a subject by determining the level of one or more cardiac related (poly)peptide biomarkers in the interstitial fluid (ISF) from the subject, and comparing the determined level to a reference value. Further this invention refers to the use of ISF in the methods described herein.

The invention relates to a microneedle device for subcutaneous tissue interstitial fluid extraction and a sampling method, and belongs to the technical field of tissue interstitial fluid extraction.The microneedle device comprises a fixator body, the fixator body comprises an upper fixator and a lower fixator, the two sides of the upper fixator are connected with the two sides of the lower fixator through elastic pieces, and an array channel is formed in the upper fixator; the pressure detection head comprises a pressure detection shell and a sheet-shaped dynamometer probe, a hollow column assembly matched with the array channel is arranged at the bottom of the pressure detection shell, the hollow column assembly comprises a plurality of hollow columns, columns are arranged in the hollow columns in a matched mode, and a containing groove is formed in the pressure detection shell; the microneedle array structure comprises a microneedle structure shell, a capillary tube and a microneedle, a tube body matched with the array channel is arranged at the bottom of the microneedle structure shell, a mounting hole is formed in the tube body, the capillary tube is arranged in the mounting hole, the microneedle is arranged at the bottom of the tube body, and the microneedle is communicated with the capillary tube.

The invention discloses a patient-specific bile duct cancer chip and a method thereof in dynamic drug resistance evolution analysis, and relates to the technical field of biomedicine, the patient-specific bile duct cancer chip comprises a central tumor microenvironment chamber, the central tumor microenvironment chamber is internally provided with a plurality of independent three-dimensional culture chambers, and patient-derived cells are embedded and loaded in a matrix; the perfusion channel network is connected with the plurality of three-dimensional culture chambers to form a flow channel and is used for continuously perfusing a culture medium, a medicine or a stromal cell suspension and simulating blood flow and interstitial fluid flow, and the microenvironment regulation and control unit is integrated with the perfusion channel network to control the flow channel. The chip provided by the invention integrates tumor cells, cancer-related fibroblasts and endothelial cells, so that the tumor cells, the cancer-related fibroblasts and the endothelial cells grow in a 3D matrix to form organoids with spatial structures and interstitial infiltration, and the microfluidic perfusion simulates blood / interstitial fluid flow, so that the drug test environment is closer to the actual situation of a human body, and the obtained data has higher physiological prediction value.

The invention relates to the technical field of biomedical sensing and wearable health monitoring, and discloses a photoelectrochemical sensing microneedle, a preparation method thereof, a monitoring system and a detection method. The sensing microneedle comprises a swellable dual-network hydrogel microneedle substrate, a three-dimensional conductive network and a semiconductor nanostructure, wherein the three-dimensional conductive network is composed of high-length-diameter-ratio metal nanowires with a local surface plasma resonance effect, and the semiconductor nanostructure is compounded on the surfaces of the metal nanowires and forms a Schottky heterojunction. The mechanical constraint of the rigid polymer skeleton and the high length-diameter ratio of the metal nanowire cooperate to ensure the continuity of the conductive network in a swelling state. Under the irradiation of exciting light, hot electrons are migrated to the surface of a semiconductor through a Schottky junction built-in electric field to generate reactive oxygen free radicals, non-enzymatic catalytic oxidation is carried out on a target analyte in interstitial fluid under zero bias voltage or low bias voltage, a photocurrent signal is generated, and integration of minimally invasive sampling and in-situ high-selectivity detection is realized.

Non-invasive in vivo measurement, by Raman spectroscopy, of analyte e.g. glucose present in e.g. interstitial fluid in the skin of a subject. A plurality of vertical-cavity surface-emitting lasers (VC

The present invention generally relates to systems and methods for delivering and / or receiving a substance or substances such as blood from subjects. In one aspect, the present invention is directed to devices and methods for receiving or extracting blood from a subject, e.g., from the skin and / or from beneath the skin, using devices containing a substance transfer component (for example, one or more needles or microneedles) and a reduced pressure or vacuum chamber having an internal pressure less than atmospheric pressure prior to receiving blood. In some embodiments, the device may contain a “snap dome” or other deformable structure, which may be used, at least in part, to urge or move needles or other suitable substance transfer components into the skin of a subject. In some cases, for example, the device may contain a flexible concave member and a needle mechanically coupled to the flexible concave member such that the needle may be urged or moved into the skin using the flexible concave member. Other aspects of the present invention are directed at other devices for receiving blood (or other bodily fluids, e.g., interstitial fluid), kits involving such devices, methods of making such devices, methods of using such devices, and the like.

Transdermal sampling and analysis devices, methods, and systems are provided. The transdermal sampling and analysis device may include a base structure with a disruptor and a first electrode array, a lid structure with a second electrode array, and an adhesive structure containing a hole to direct interstitial fluid to at least one of the first and second electrode arrays. The device may also include at least one conductive trace configured for external connection to one or more of a voltage / current source and a current detector, and an electrically conductive material that passes through a hole in the adhesive layer, providing a conductive path between the base structure and the lid structure. Methods and systems for manufacturing a plurality of biosensing chips that may be used in the transdermal sampling and analysis devices are also provided.

The fully-embedded self-driven interstitial fluid collecting type dilator comprises a dilation bag, an interstitial fluid filtering bag is arranged on the left side of the dilation bag, the interstitial fluid filtering bag is of an oblate sphere structure after being deformed, and the bag wall is a micropore dialysis filter membrane; a liquid inlet in the left end of the expansion bag is communicated with a liquid outlet in the right end of the interstitial fluid filtering bag through a liquid guide pipe, a valve control mechanism is arranged in the middle of the liquid guide pipe, and a negative pressure generating mechanism used for generating negative pressure in the interstitial fluid filtering bag is arranged in the middle of the liquid guide pipe; according to the full-embedded self-driven tissue fluid collection type expander, the built-in semi-permeable negative pressure generation mechanism is used for collecting and filtering tissue fluid, the tissue fluid is controllably pumped into the expansion bag through the valve control mechanism, and the self-expansion extrusion pump or the electric suction pump can be selected as the negative pressure generation mechanism. Safe expansion which does not need external puncture water injection, does not depend on external energy, is controllable in expansion speed and can quickly release pressure in an emergency state is achieved.

The present invention relates to an apparatus and method for non-invasive in vivo measurement, by Raman spectroscopy, of glucose present in interstitial fluid in the skin of a subject. The apparatus c