10 results about "Stratum basale" patented technology

The present invention relates to a method for the in vitro characterization and quantification of proliferative cells in an epidermal fragment, comprising the following steps: 1) Culturing the epidermal fragment on a support, 2) Detaching the epidermal fragment from the support, 3) Performing immunostaining on the epidermal fragment, 4) Acquiring images of the basal layer of the epidermal fragment over its entire surface, 5) Reconstructing an image representing the entire basal layer of the epidermal fragment, 6) Selecting the immunostained nuclei of the proliferative cells by image processing, 7) Quantifying cell division by counting the nuclei through image analysis. The present invention also relates to using the method to evaluate the effect of a product or active ingredient on epidermal cell regeneration. Figure 1

The present invention provides, as a technique that can be used for obtaining a skin organoid or a skin tissue structure that skillfully mimics the structure and function of the skin of a living body from stem cells, a skin organoid comprising cells that are derived from pluripotent stem cells and have the ability to differentiate into cells constituting the skin, the skin organoid having a lumen, the skin organoid having an epidermal cell layer comprising cells having the ability to differentiate into epidermis on the surface facing the lumen, and the skin organoid having a mesenchymal cell layer, on the outside of the epidermal cell layer, comprising cells having the ability to differentiate into dermis or subcutaneous tissue. The epidermal cell layer is configured by laminating, in this order from the lumen side, a granular layer comprising cells that express loricrin, a spinous layer comprising cells that express KRT10, and a basal layer comprising cells that express KRT5 and p63, and has hair pegs invaginating the mesenchymal cell layer.

The invention discloses a mussel byssus gland mucoprotein subunit and a preparation method and application thereof.The preparation method comprises the steps that a mussel byssus gland serves as a raw material, the outer layer of the byssus gland is removed through neutral protease hydrolysis, then the secondary outer layer of the byssus gland is removed through pepsase hydrolysis, a byssus gland core is obtained, a urea solution is added to the byssus gland core for homogenization treatment, and the mussel byssus gland mucoprotein subunit is obtained; the preparation method comprises the following steps: centrifuging to remove insoluble impurities, adding dithiothreitol into supernate to obtain crude extract of the byssus mucoprotein, performing microfiltration to remove biological impurities, performing nanofiltration on microfiltrate to obtain byssus mucoprotein subunit liquid, and performing spray drying or freeze drying on collected liquid to obtain high-solubility mussel byssus mucoprotein subunit powder. The high-solubility mussel byssus mucoprotein subunit is obtained through a double-enzyme urea nanofiltration engineering reduction method, has the effects of thickening a skin basal layer, promoting hair growth, inducing skin regeneration and the like, and is applied to the field of skin beauty treatment and the field of biological medical instruments.

Cosmetic or dermatological use of an extract of Dendrobium officinale to maintain and / or increase skin thickness. The present invention relates to the new cosmetic or dermatological use of an extract of Dendrobium officinale and / or a composition comprising it to maintain and / or increase skin thickness, preferably of the epidermis, more preferably of the basal layer, the spinous layer and / or the granular layer of the epidermis.

When the oxygen isolation type photodynamic therapy device is used, the light-transmitting oxygen isolation type equipment covers the skin lesion surface of a patient to form an oxygen isolation layer, the tissue oxygen concentration of the epidermal layer and the dermal superficial layer of the patient can be reduced, and therefore the singlet oxygen photochemical generation rate of the area is reduced, and the photodynamic therapy effect is improved. The level of photochemical pain reaction caused by singlet oxygen during treatment of a patient is reduced; meanwhile, the method is cooperated with a light modulation technology, so that the singlet oxygen accumulation damage ratio of the targeted tissue and the non-targeted tissue can be further improved. Meanwhile, target cells of the erythema are located on capillary walls, target cells of skin precancerous lesions are located near a basal layer, and the oxygen concentration of the places is higher than that of an epidermal layer, so that the target selectivity of photodynamic therapy can be improved; the material can be used for hypoxia photodynamic therapy of nevus flammeus, skin precancerous lesions and other skin diseases.

The present invention relates to a method for the in vitro characterization and quantification of proliferative cells in an epidermal fragment, comprising the following steps: 1) Cultivating, on a support, the epidermal fragment, 2) Detaching the epidermal fragment from the support, 3) Carrying out immunolabeling on the epidermal fragment, 4) Acquiring images of the basal layer of the epidermal fragment over its entire surface, 5) reconstructing an image representing the entire basal layer of the epidermal fragment, 6) Selecting the immunolabeled nuclei of the proliferative cells by image processing, 7) Quantifying cell division by counting the nuclei by image analysis. The present invention also relates to a use of the method for evaluating the effect of a product or an active ingredient on epidermal cell regeneration. Figure 1

The present invention relates to a wound covering material composition capable of promoting the healing effect of a skin wound by means of the growth of stem cells on the epidermal basal layer of the skin. The invention provides a wound covering material composition, which comprises the following components in percentage by weight: 60 to 70 percent of glycerol; 28 to 39 weight percent of honey; 0.2 wt% to 3.0 wt% of fucosan; 0.2 to 3.0 wt% of an olive leaf extract; and 0.1 to 0.3 wt% of xanthan gum.

By utilizing the characteristic that acyclovir cannot corrode normal skin when being externally applied, the acyclovir is mixed with traditional Chinese medicine composite components which are capable of softening cutin, high in permeability, expanding microcirculation and free of ablativity, so that the acyclovir can be applied trustingly, sufficiently, timely, expanded and repeatedly, the effective components of the acyclovir can penetrate into and reside on a basal layer of epidermis for a long time and continuously act on HPV strains, and the treatment effect of the acyclovir is improved. And finally clearing. A large piece of HPV verruca which is lately bred for many years can be removed, and the total treatment effect can reach 90% or above; within a considerable period when no specific medicine exists at present, the ointment can be used as a major ointment for treating the HPV verruca vulgaris (can be matched with an antiviral medicine at the same time).

The invention relates to the technical field of biological medicine and cosmetic preparations, and discloses a stem cell exosome polypeptide composite anti-wrinkle whitening skin care product and a preparation method thereof. According to the composition, a CD63 antibody is used for performing directional modification on an exosome membrane surface from deer bone marrow mesenchymal stem cells, so that targeted delivery of epidermal basal layer stem cells is realized; meanwhile, the whitening active polypeptide is entrapped in the temperature response type nano-liposome formed by phospholipid / poly (N-isopropylacrylamide), so that phase change is generated to trigger slow release. And performing membrane fusion on the two to form composite vesicles to prepare a W / O emulsion formula. Compared with a single component, the compound preparation prepared by the invention has the advantages that the synthesis of fibroblast collagen I can be improved by 22.3%, the tyrosinase inhibition activity is 58.3%, the synergistic interaction is realized in the aspects of wrinkle resistance and whitening, the process is simple and convenient, the components are stable, and the compound preparation is suitable for high-end skin care application.

The present application provides a soluble microneedle patch. The needle body of the soluble microneedle patch of the present application can directly act on the epidermal basement layer through the stratum corneum, accelerating the transdermal absorption of effective components. Moreover, the active ingredients in the central layer and the outer layer of the microneedle can synergistically enhance the absorption and utilization effect of multiple components. In addition, unlike the liquid environment of traditional emulsions, the microneedle is in solid form, which can minimize the reactivity of active ingredients. The optimized soluble microneedle patch surface spraying scheme in the present application can spray and solidify the substances that are unstable in contact in a very short time on the surface of the microneedle and the patch base, thereby forming a formula form that flexibly matches the central layer and the outer layer of the soluble microneedle needle body, and realizing the improvement in function.