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A double-course compound cell growth factor hydrogel and its preparation method and application

A growth factor and hydrogel technology, which is applied in the direction of drug combinations, pharmaceutical formulas, medical preparations containing active ingredients, etc., can solve the problems of optimization and long skin repair time, and achieve easy absorption, safety and quick effect, no Toxic effects

Inactive Publication Date: 2018-07-24
王悦
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, at present, all the hydrogel products with a single formula are available on the market. Existing products often add a variety of excipients that are irritating to the skin. Moreover, these products have not been optimized for the repair mechanism of different layers of skin. It took a long time, in view of this, the case arises from

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

10mmol / L pH6.8 sterile phosphate buffer

Make up to 1000ml

[0037] No. 1 hydrogel preparation method: 1. 1.0% by mass of organic vegetable glycerin, 0.05% by mass of hyaluronic acid, 3.0% by mass of mannitol, 1.0% by mass of menthol, and 0.2% by mass of decylmethylsulfoxide %, poloxamer 407 mass percentage 20%, swelling and stirring with 10mmol / L pH6.8 sterile phosphate buffer at 4°C, then autoclaving at 121°C for 20 minutes, and cooling;

[0038] 2. Dissolve human fibroblast growth factor hFGF-21 5000IU / g, human epidermal growth factor hEGF40000IU / g, and allantoin mass percentage 2.0% in 10mmol / L pH6.8 sterile phosphate buffer, and use 0.22 um membrane filter sterilization;

[0039] 3. Under aseptic conditions, when the hydrogel obtained in step 1 is cooled to 4°C, mix the solution obtained in step 2 with it and stir evenly, and dispense it into a hose sterilized by ethylene oxide.

[0040] No. 2 hydrogel component

Dosage (mass percentage%)

H...

Embodiment 2

Make up to 1000ml

[0047] No. 1 hydrogel preparation method: 1. 3.0% by mass of organic vegetable glycerin, 0.05% by mass of hyaluronic acid, 1.0% by mass of mannitol, 2.0% by mass of menthol, and 0.1% by mass of decylmethylsulfoxide %, poloxamer 407 mass percentage 20%, swollen and stirred with 10mmol / L pH6.8 sterile buffer solution at 4°C, then autoclaved at 121°C for 20 minutes, and cooled;

[0048] 2. Human fibroblast growth factor hFGF-21 10000IU / g, human epidermal growth factor hEGF20000IU / g, allantoin mass percentage 2.0% were dissolved in a sterile buffer, and sterilized by filtration with a 0.22um filter membrane;

[0049] 3. Under aseptic conditions, when the hydrogel obtained in step 1 is cooled to 4°C, mix the solution obtained in step 2 with it and stir evenly, and dispense it into a hose sterilized by ethylene oxide.

[0050] No. 2 hydrogel component

Dosage (mass percentage%)

Human Keratinocyte Growth Factor hKGF-2

10000IU / g

...

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PUM

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Abstract

The invention discloses a hydrogel containing human fibroblast growth factor hFGF‑21 and human epidermal growth factor hEGF components, which can repair and heal the dermis and basal layer; the invention also discloses a hydrogel containing human keratinocytes A hydrogel of the growth factor hKGF‑2, human epidermal growth factor hEGF component, which targets the repair and healing of the epidermis and stratum corneum. The invention also discloses the preparation method and application of the two hydrogels. The invention optimizes the healing mechanism of wound tissue in different layers of the skin, so that the repairing ingredients of the formula have no irritation to the skin, no toxic and side effects, are easy to absorb, shorten the healing time, and are safe and effective.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to a double-course compound cell growth factor hydrogel and its preparation method and application. Background technique [0002] Skin cell growth factors, including human fibroblast growth factor hFGF-21, human epidermal growth factor hEGF, human keratinocyte growth factor hKGF-2, etc., are polypeptides normally secreted by the human body, and play a role in the growth, differentiation and regeneration of skin tissue cells It plays an important physiological role in migration and migration, and has been widely used in skin damage repair. For example, application number 200510036465.3 discloses a recombinant human basic fibroblast growth factor gel and its preparation method, and 200510009218.4 discloses recombinant human basic fibroblast growth factor gel Fibroblast growth factor gel and its preparation method, 200610112958.5 disclosed recombinant human acidic fibroblas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/18A61K9/06A61P17/00A61L15/44A61L15/42
Inventor 王悦
Owner 王悦
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