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81 results about "Recombinant human basic fibroblast growth factor" patented technology
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RECOMBINANT HUMAN-BASIC FIBROBLAST GROWTH FACTOR (rh-bFGF) AND PHARMACEUTICAL COMPOSITION COMPRISING rh-bFGF
ActiveUS20200157164A1Senses disorderPeptide/protein ingredientsPharmaceutical drugRecombinant human basic fibroblast growth factor
Provided are a mutated nucleic acid molecule of a recombinant human-basic fibroblast growth factor (rh-bFGF), a pharmaceutical composition of the rh-bFGF, and a use thereof.
Owner:ZHUHAI ESSEX BIO PHARMA
Recombined human alkaline fibroblast growth factor gelling agent and process for preparing the same
InactiveCN1733294AReduce formationEffective function recoveryPeptide/protein ingredientsPharmaceutical delivery mechanismMANNITOL/SORBITOLEffective treatment
The invention relates to a recombinant human basic fibroblast growth factor gelling agent and the process for preparation, which comprises recombinant human basic fibroblast growth factor (rh-bFGF) stock solution 0.1-100% (W / W), medicinal auxiliary material 0-99.9% (W / W), the stock solution comprises effective treatment dose of recombinant human basic fibroblast growth factor (rh-bFGF) 0.01-1% (ug / g), NaCl 0.01-20% (W / W), phosphates cushioning liquid 2m Mol - 200m Mol, and right amount of water for injection, the medicinal auxiliary materials can be selected from: carbomer 940NF, sodium hyaluronate, trehalose, mannitol, dextran 40 and sodium heparin.
Owner:朗肽生物制药股份有限公司
Regenerated human alkali fiber-cell growth factor gel former and preparing method
InactiveCN1814279AReasonable matrixNon-irritatingPeptide/protein ingredientsPharmaceutical delivery mechanismDiseaseSolubility
The invention relates to a rh-bFGF gel and the making method that is a drug used to cure wound, canker, scald, and burn. It is compounded by 146 amino acids. The main drug is rh-bFGF, and gel basal material includes thickener, humectant, antiseptic, stabilizer, pH regulator, and water. The manufacture method includes the following steps: mixing the thickener, humectant, antiseptic, stabilizer, pH regulator, and water according to certain ratio, whisking to equal to make water solubility gel basal material, adding rh-bFGF after high pressure sterilization, mixing equally to make gel and separately packaging into aluminum mold tube to make drug using rh-bFGF gel products.
Owner:北京双鹭立生医药科技有限公司
Preparation method of graphite/carbon nano fiber biomedical externally applied non-woven fabric
ActiveCN107137753ASimple processEasy to operateAbsorbent padsNon-woven fabricsElectrospinningCarbon nanofiber
The invention provides a preparation method of graphite / carbon nano fiber biomedical externally applied non-woven fabric, which belongs to the field of medical materials used for surgery. Graphite powder is embedded into a polymer fiber shell layer or core layer by coaxial electrostatic spinning technique, peroxidation and carbonizing treatment are carried out to obtain porous graphite / carbon nano fiber non-woven fabric with large specific surface area, strong air permeability and high strength, and medical liquid containing recovery liquid or externally applied recombinant human basic fibroblast growth factor is sprayed on the surface of the non-woven fabric to obtain graphite / carbon nano fiber biomedical externally applied non-woven fabric for promoting wound surface healing. The preparation method of the invention has the characteristics of simple process, convenience, environmental friendliness, low cost, adaptability for large-scale production, and so on. The graphite / carbon nano fiber non-woven fabric prepared by the method of the invention has high mechanical strength, high flexibility, good durability, rich pores, and high liquid absorption capacity, does not stick to the wound surface and can promote the wound surface healing, and can be widely used for treatment of traumas such as wound, ulcer, scald and extensive burn.
Owner:重庆锦添翼新能源科技有限公司
Serum-free culture medium, preparation method thereof and culture method of mesenchymal stem cells
ActiveCN110923196AImprove proliferative abilityImprove performanceCulture processSkeletal/connective tissue cellsPolyvinyl alcoholHydrocortisone
Owner:GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
Stem cell culture medium
InactiveCN105200008ADoes not affect potentialHigh speedNervous system cellsSkeletal/connective tissue cellsSodium bicarbonateHuman platelet
The invention discloses a stem cell culture medium. The stem cell culture medium comprises an improved DMEM (Dulbecco's Modified Eagle Medium) / F12 basal culture medium and an additive, wherein the additive comprises sodium bicarbonate, selenium amino acid chelate, recombinant human insulin growth factors, recombinant human basic fibroblast growth factors, recombinant human lactoferrin, ascorbic acid, recombinant human platelet-derived growth factors, recombinant human vascular endothelial cell growth factors, octacosanol, polyvinyl alcohol, polyvinylpyrrolidone and recombinant human epidermal growth factors. According to the stem cell culture medium, the potential of stem cells is not influenced while the stem cells can be proliferated rapidly, the proliferation speed of the stem cells is increased by 3-5 times compared with a common culture medium, and further, the stem cell culture medium can be used for culturing the stem cells of various kinds of tissue and has excellent applicability; the cultured stem cells have high differentiation capability, can be differentiated into multiple functional cells and have very high scientific research and medical application values, culture medium components are exact, the quality is stable, and accordingly, the cultured stem cells are not likely to generate human body rejection reaction after transplanting.
Owner:XINXIANG MEDICAL UNIV
Neural stem cells medium and method for performing human neural stem cells in-vitro long-term culture and amplification by using neural stem cells medium
ActiveCN105062972AGenetic stabilitySolve the easy differentiation of in vitro cultureNervous system cellsInsulin activityCuticle
The invention relates to a neural stem cells medium and a method for performing human neural stem cells in-vitro long-term culture and amplification by using the neural stem cells medium. The neural stem cells medium comprises the following ingredients by weight proportion: 100-1000 micrograms of heparin sodium, 10-100 micrograms of vitamin E, 5-50 milligrams of insulin human recombinant, 0.5-5 milligrams of putrescine, 2-10 micrograms of sodium selenite, 2-10 milligrams of human transferrin, 2-10 micrograms of progestin, 300 milligrams of L-glutamine, 5.9 grams of 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid, 10-100 micrograms of recombinant human epidermal growth factors, 10-100 micrograms of recombinant human basic fibroblast growth factors, 20-200 milligrams of vitamin C glucoside and 40,000-400,000 IU (international unit) of gentamicin. By the neural stem cells medium, the technical problems that human neural stem cells are easy to differentiate when cultured in vitro and long-term culture and amplification are difficult to implement are solved.
Owner:ZHEJIANG ORIGIN BIOTECH
Serum-free medium for liver cancer stem cells and culture method for serum-free medium
The invention provides a serum-free medium for liver cancer stem cells and a culture method for the serum-free medium. Based on a DMEM / F12+GlutaMAXTM<-1> medium, the serum-free medium comprises a recombinant human epidermal growth factor, a recombinant human basic fibroblast growth factor-10, an insulin No.1 growth factor, a serum-free complementary nutritional ingredient, heparin and a penicillin and streptomycin double-resistant solution. The serum-free medium has the effects as follows: 1, three special growth factors are added to the serum-free medium in a corresponding proportion; direct serum-free culture is realized, so that liver cancer cells can be pelletized, the pelletizing rate is high, and continuous and stable passage can be realized; 2, the growth factors in the serum-free medium keep a small group of cells in an undifferentiated state; the serum-free medium and the culture method have wide prospect in fundamental research and clinical application of serum-free culture of the liver cancer stem cells and provide rich experiment materials for research of the liver cancer stem cells.
Owner:广西壮族自治区肿瘤医院
Separation and cultivation method of human adipose stem cells
ActiveCN108220230ANormal growthNormal metabolismCell dissociation methodsCulture processSerum free mediaParenchyma
The invention discloses a separation and cultivation method of human adipose stem cells. The method comprises the following steps: first, digesting a fat suction matter by using a Liberase digestive enzyme solution, neutralizing digestive enzyme after digestion is ended, centrifuging, filtering, and further removing parenchyma cells by using a lymphocyte separation solution so as to obtain the human adipose stem cells; second, cultivating the human adipose stem cells by using a serum-free medium, wherein following components are added in the serum-free medium: a recombinant human epidermal growth factor, a recombinant human basic fibroblast growth factor, a recombinant human transforming growth factor-beta, a recombinant human platelet-derived growth factor-BB, a recombinant human stem cell factor, reduced glutathione, coenzyme A, biotin, MEM vitamin solution, MEM amino acid solution, MEM non-essential amino acid solution, a GlutaMAX additive, insulin-transferrin-selenium solution andgentamicin. The method can remarkably increase the yield of adipose stem cells and improve the activity of the adipose stem cells, so as to obtain more adipose stem cells with proliferation ability.
Owner:SHANGHAI LIFE SCI & TECH CO LTD
Recombinant human basic fibroblast growth factor gel without bacteriostatic agent and preparation method thereof
InactiveCN104984326AAvoid stimulationAvoid pollutionSenses disorderPeptide/protein ingredientsMicroorganismRecombinant human basic fibroblast growth factor
The invention provides a recombinant human basic fibroblast growth factor gel without a bacteriostatic agent. The recombinant human basic fibroblast growth factor gel comprises the following components: 1*10<6> to 9*10<6> IU of recombinant human basic fibroblast growth factors, 0.01 to 5.0 g of albumen stabilizers, 0.1 to 20.0 g of thickeners, 0.1 to 10.0 g of pH regulators, 0.1 to 10.0 g of buffering agents and 0.1 to 50.0 g of humectants, and water for injection is added to a constant volume of 1000 ml. The invention further provides a preparation method of the recombinant human basic fibroblast growth factor gel without the bacteriostatic agent. The recombinant human basic fibroblast growth factor gel without the bacteriostatic agent is disposable, so as to prevent eyes from being stimulated by the bacteriostatic agent and avoid microbial contamination which possibly occurs in the processes of use and storage.
Owner:ZHUHAI ESSEX BIO PHARMA
A kind of subcutaneous soft tissue filling localized fibrin compound that can be used for facial beauty and its preparation method
The invention relates to a facial fibrous protein composite filled and positioned in subcutaneous soft tissues, and a preparation method thereof. The composite comprises the following raw materials by weight: 10.0 to 95.0g of five-time autogenous concentrated plasma, 0.001 to 0.10g of calcium chloride, 1.0 to 15.0g of sodium sulfite, 0.005 to 0.5g of recombinant human vascular endothelial growth factor freeze-dried powder, 0.005 to 0.5g of recombinant human basic fibroblast growth factor freeze-fried powder, 0.1 to 10.0g of amino-silanized ferroferric oxide magnetic particle, and 5.0 to 90.0g of 0.1mol / L phosphate buffered saline injection. The autogenous concentrated plasma can form a stable composite fibrous protein scaffold in several hours under the double actions of calcium chloride and sodium sulfite to meet the requirement of plastic surgery; meanwhile, the composite can achieve the accurate positioning and filling effect under the action of an external magnetic field; moreover, the composite does not have irritability, and is high in safety and easy to prepare, has a good filling effect, promotes the proliferation of surrounding cells in which the composite is filled, restores tissues of the depression part and achieves the strengthening, filling and restoration effects.
Owner:董萍 +1
Embryo cattle serum substitute without serum for animal cell culture
InactiveCN1800368ALow costTo achieve the purpose of replacing serumAnimal cellsL929 cellHuman platelet
The invention relates to a non-serum animal cell culture used fetus cattle serum displace agent, which is formed by recombination human insulin growth factor-1:0.1g-0.5g / l, recombination human platelet sourced growth factor-B:0.1g-0.6g / l, recombination human alkali desmocyte growth factor: 0.05-0.4g / l, cattle transferring: 1g-5g / l, cattle serum protein (V component): 2g-15g / l, and mercaptoethanol 0.3-0.5mol / l.
Owner:上海尚优生物科技有限公司
Sterol derivative, and preparation method and application thereof
ActiveCN106800580AThe synthesis method is simpleEasy to operateOrganic active ingredientsSenses disorderPositive controlCholesterol
The invention discloses a sterol derivative of beta-sitosterol, beta-stigmasterol and cholesterol, and is shown as a formula VI. The invention also discloses a preparation method of the sterol derivative. The invention also discloses application of the sterol derivative to the aspect of preparation of wound healing promoting medicine. By starting from easily obtained natural products, the beta-sitosterol, the beta-stigmasterol and the cholesterol are used as starting raw materials; the synthetic method is simple; better operability and reaction yield are realized. The prepared sterol derivative has the obvious wound healing promoting activity; the multiplication, migration and collagen synthesis capability on L929 mechanocytes is obviously higher than that of the raw material and positive control medicine recombinant human bFGF (basic fibroblast growth factor). Compared with protide type medicine (such as bFGF), the prepared sterol derivative has more diversified dosage forms and medication modes; the reference is provided for the application in the field of wound healing promoting. The formula VI is shown as the accompanying diagram.
Owner:CHINA PHARM UNIV
Serum-free culture medium and preparation method and application therefor
ActiveUS20180362920A1Maintain pluripotencyStrong proliferation capacityCulture processCell culture mediaFiberAdditive ingredient
Provided is a serum-free culture medium, the ingredients of the culture medium comprising 0.05-0.2 parts by volume of β-mercaptoethanol, 0.5-2 parts by volume of non-essential amino acid aqueous solution, 4-6 parts by volume of human mesenchymal stem cell culture supernatant concentrate, and 90-95 parts by volume of a-MEM / DMEM-F12 and recombinant human alkaline fibroblast growth factor of a final concentration of 5-5 ng / ml. The present culture medium is used for carrying out stem cell culture.
Owner:GUO LEI +1
Reprogramming culture medium, preparation method thereof and culture method of reprogramming cell
ActiveCN103602635AClear ingredientsImprove qualityForeign genetic material cellsSodium bicarbonateValproic Acid
The invention discloses a reprogramming culture medium, a preparation method thereof and a culture method of a reprogramming cell, wherein the reprogramming culture medium comprises a basal culture medium, a reprogramming additive 1 and a reprogramming additive 2, wherein the basal culture medium is a DMEM (Dulbecco Modified Eagle Medium) / F12 culture medium; the reprogramming additive 1 comprises sodium hydrogen carbonate, sodium selenate, recombinant human insulin, recombinant human basic fibroblast growth factors, recombinant human transferrin, ascorbic acid and hydrocortisone; the reprogramming additive 2 comprises sodium hydrogen carbonate, sodium selenate, recombinant human insulin, recombinant human basic fibroblast growth factors, recombinant human transferrin, ascorbic acid, valproic acid and sodium butyrate. The reprogramming culture medium disclosed by the invention is exact in component, stable in quality, high in reprogramming efficiency, wide in cell lineage and capable of reprogramming and culturing cells such as human skin fibroblasts, peripheral blood mononuclear cells, amniotic epithelial cells and the like.
Owner:BEIJING CELLAPY BIOTECH
Embryo cattle serum substitute without serum or animal protein for animal cell culture
The invention relates to a non-serum non-animal origin protein animal cell culture used fetus cattle serum displace agent, which is formed by recombination human insulin growth factor-1:0.1g-0.5g / l, recombination human platelet sourced growth factor-B:0.1g-0.6g / l, recombination human alkali desmocyte growth factor: 0.05-0.4g / l, polyethylene glycol (MW7000-20000), 0.04-0.15%(w / v), polyvinyl alcohol (density 1.27-1.31): 0.001-0.05%(w / v), mercaptoethanol: 0.3-0.5mol / l.
Owner:上海席诺生物技术有限公司
Serum-free culture medium for mesenchymal stem cells
InactiveCN110257328APromote in vitro proliferationCulture processSkeletal/connective tissue cellsSodium bicarbonateLithium chloride
The invention provides a serum-free culture medium for mesenchymal stem cells. The serum-free culture medium specifically comprises a lipid mixture, glutamine, hydrocortisone, zinc sulfate, sodium butyrate, ferric citrate, vitamin C, recombinant epidermal growth factors, recombinant alkaline fibroblast growth factors, RGD oligopeptides, palmitoyl tripeptide-5, palmitoyl tetrapeptide-7, lithium chloride, L-glutathione, a soybean pancreatin inhibitor, beta-mercaptoethanol, ethanolamine, sodium selenite, Hepes, sodium bicarbonate and a basic culture medium. The serum-free culture medium is free of human and animal resources, clear in chemical component, rich in nutrition, capable of achieving UC-MSCs rapid proliferation, and capable of solving the problem of cells number insufficiency and maintaining the biological features and immunophenotyping stability of the mesenchymal stem cells.
Owner:GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD +1
Endothelium culture system
InactiveCN105647851AIncrease growth activityInhibitory activityCulture processArtificial cell constructsInsulin-like growth factorVitamin C
The invention discloses an endothelium culture system. The endothelium culture system comprises an endothelium culture medium and an endothelium additive, wherein the endothelium additive comprises components by concentration as follows: 5 ng / ml of a recombinant human epidermal growth factor, 10 ng / ml of hydrocortisone, 10 ng / ml of a recombinant human vascular endothelial growth factor, 5 ng / ml of a recombinant human basic fibroblast growth factor, 5 ng / ml of an R3 insulin-like growth factor, 50 mu g / ml of vitamin C, 50 mu g / ml of heparin, 10 mu g / ml of recombinant human insulin and 5 mu g / ml of human total iron transferrin. The endothelium culture system can promote growth activity of great vascular endothelial cells in a 5% serous environment, partially inhibit activity of fibroblasts and smooth muscle cells and reduce parenchyma cell pollution.
Owner:ICELL SHANGHAI BIOSCI CO LTD
Skin care product with effect of removing stretch marks and preparation method thereof
ActiveCN103860449AIncrease supplyHigh activityCosmetic preparationsToilet preparationsHalloysiteFiber
The invention discloses a skin care product with an effect of removing stretch marks and a preparation method thereof. The skin care product consists of nidus vespae, fructus forsythiae, red flower, peach blossom, ginseng, gastrodia elata, dragon bone, red halloysite, witch hazel, coptis chinensis, codonopsis pilosula, bletilla striata, snail meat, a recombinant human epidermal growth factor, a recombinant human basic fibroblast growth factor, vitamin C, hyaluronic acid, sweet almond oil, rosehip seed oil, 1,3-butanediol, allantoin and auxiliary materials. The skin care product is a comprehensive and complete system and infiltrates into skin tissues through the synergistic effect between the traditional Chinese medicine and multiple nutrients, so that the fibroblast genes are activated, the cell viability is enhanced, and cell proliferation is promoted. Moreover, repair and synthesis of collagens and elastins are promoted, the skin resilience and elasticity are improved, and the skin care product has the effects of diminishing inflammation, relieving itching and reducing pigments, so that the aim of removing the stretch marks is achieved.
Owner:GUANGZHOU YUANDA BIOTECH CO LTD
Basic fibroblast growth factor recombining capsule and its prepn process
InactiveCN1548148AActively repair gastric ulcer woundsPeptide/protein ingredientsDigestive systemMANNITOL/SORBITOLFreeze-drying
The recombinant human basic fibroblast growth factor capsule includes recombinant human basic fibroblast growth factor freeze dried powder 0.1-100 wt% and medicinal stuffing material 0-99.9 wt%. The preparation process includes dissolving mannitol, human blood albumin and recombinant human basic fibroblast growth factor in distilled water, abacterial filtering, freeze drying to obtain powder, sieving, mixing with stoved and sieved mannitol in certain ratio and capsulizing. The capsule is used in treating gastric ulcer in one completely new path different from traditional gastric ulcer treating medicines.
Owner:ZHUHAI ESSEX BIO PHARMA
Serum-free medium inducing differentiation of umbilical cord mesenchymal stem cell into insulin-secretion-like cell and preparation method and use thereof
ActiveUS20190264179A1Inhibit cell growthQuick distinctionPancreatic cellsCulture processRecombinant human basic fibroblast growth factorSerum free media
Provided is a new type serum-free medium. The medium comprises: DMEM with high glucose (the content of glucose being 4.5 g / L), B27, recombinant human basic fibrolast growth factor (b-FGF), nicotinamide, N-2, vinblastine III (conophylline), non-essential amino acid (NEAA), heparin, epidermal growth factor (EGF), hepatocyte growth factor (HGF), a serum replacement (SR), an insulin-transferrin-selenium complex (ITS), and pentagastrin. Inducing differentiation of mesenchymal stem cells into insulin-secretion-like cells can be achieved in six days in one step using the medium.
Owner:LI CHENG +2
Novel cell cryopreservation reagent
The invention discloses a novel cell cryopreservation reagent which comprises, 100ng of recombinant human epidermal growth factors, 500ng of recombinant human basic fibroblast growth factors, 300ng of recombinant human hepatocyte growth factors, 0.5ml of DMSO (dimethyl sulfoxide), 2ml of recombinant human serum albumin and 8ml of DMEM (Dulbecco's modified eagle medium). The novel cell cryopreservation reagent has the advantages that the novel cell cryopreservation reagent is free from animal components and safe and reliable to use, components are completely definite, quality control is facilitated, the reagent is ready to use and can be directly used for cell resuspension after cell counting, the reagent is more convenient to operate without additional preparation, applicable cell types are unlimited, and cells can be maintained in long-term safe storage in a -196 DEG C liquid nitrogen environment.
Owner:北京焕生汇生物技术研究院有限公司
Skin care product
ActiveCN1813663AImprove the living microenvironmentSpeed up the replacement of old and newCosmetic preparationsToilet preparationsWrinkle skinWhole body
The present invention provides a skin care product. Its composition mainly includes the following components: (by wt%) recombinant human epidermic cell growth factor 5X10 to the power-7-5X10 to the power-4%, recombinant human basic fibroblast growth factor 3X10 to the power-7-7X10 to the power-4%, zinc ion 1.3X10 to the power-7-6.8X10 to the power-4%, calcium ion 0.01-1.5%, magnesium ion 0.05-5.0%, N-2-hydroxyethylpiperazine-N'-2-ethyl sulfonic acid 0.01-2.0%, L-glutamine 0.01-10.0% and the rest is auxiliary material. Said invention can quickly improve living microenvironment of skin cell, can make skin become tender and rosy and can eliminate wrinkles and various spots, etc.
Owner:罗海哨
Collagen-like protein-human basic fibroblast growth factor fusion protein and preparation method thereof
InactiveCN105859892AAntibody mimetics/scaffoldsNucleic acid vectorStreptococcus pyogenesProtein length
The invention provides collagen-like protein-human basic fibroblast growth factor fusion protein. The whole length of the fusion protein is 488 amino acids, and the nitrogen end is modified collagen-like protein Scl2-M of a streptococcus pyogenes source; the protein length of the Scl2-M is 327 amino acids, the carbon end is a human basic fibroblast growth factor hbFGF, and the protein length of the hbFGF is 155 amino acids. An enterokinase restriction enzyme cutting site exists between two peptide fragments. The preparation method comprises the steps of establishment of an expression vector of the collagen-like protein-human basic fibroblast growth factor fusion protein, fermentation of the fusion protein, purification of recombined human basic fibroblast growth factors and collagen-like protein and measurement of bioactivity. By means of the method, the human basic fibroblast growth factors and the collagen-like protein can be efficiently produced at the same time, and therefore a foundation is laid for scale preparation of the human basic fibroblast growth factors and application of the collagen-like protein.
Owner:ZHEJIANG UNIV
Method and kit for efficiently promoting adipose-derived stem cells to proliferate
PendingCN106957818ATo achieve the effect of breast enlargementCulture processCell culture supports/coatingTransferrinInsulin humulin
The invention provides a method for efficiently promoting adipose-derived stem cells to proliferate. The method is characterized in that a cell culture plate or a culture dish coated by gelatin or a cell culture bottle coated by gelatin is adopted to efficiently promote the adipose-derived stem cells to proliferate and grow in fat under combined culture of a recombinant human epithelial growth factor, a recombinant human platelet derived growth factor, a recombinant human basic fibroblast growth factor, insulin, transferrin, fetal calf serum and the like, so that cells from 50mL fat can be cultured to obtain 109 adipose-derived stem cells or more within 15 days, and therefore, great cell population for clinically treating diseases and medical cosmetology can be met, secondary liposuction is avoided and pains caused by liposuction are reduced. According to the method, the invention further provides a kit for efficiently promoting the adipose-derived stem cells to proliferate, and the kit can be used for efficiently promoting the adipose-derived stem cells to proliferate and grow.
Owner:泰州市数康生物科技有限公司
Novel cell recovery agent
ActiveCN106635952AEasy to useClear ingredientsAnimal cellsCulture processAdditive ingredientReady to use
The invention discloses a novel cell recovery agent. The novel cell recovery agent comprises the following ingredients: 100 ng of a recombinant human epidermal growth factor, 200 ng of a recombinant human basic fibroblast growth factor, 3000 ng of vitamin D, 2 ml of a recombinant human serum albumin, and 8 ml of an RPMI-1640 culture medium. The novel cell recovery agent has the beneficial effects that (1) the novel cell recovery agent does not contain animal ingredients and is safe and reliable to use; (2) the ingredients are completely clear and definite, and the quality is convenient to control; (3) the novel cell recovery agent is a ready-to-use type, and the operation is more convenient; (4) the applicable cell type is not limited; and (5) better cell viability and thawing rate are achieved when the cell is recovered.
Owner:焕生汇生物基因技术(北京)有限公司
Method for preparing adipose-derived stem cells with function of high collagen expression and product prepared from adipose-derived stem cells
PendingCN108753705ATo achieve the effect of breast enlargementCulture processSkeletal/connective tissue cellsHuman typeLiposuction
The invention provides a method for preparing adipose-derived stem cells with the function of high collagen expression. The method comprises the following steps: a recombinant human type-IV collagen alpha 3 coated cell cultivator is used, under joint culture of recombinant human epithelial growth factors, recombinant human basic fibroblast growth factors, vitamin E and argania spinosa kernel oil,the adipose-derived stem cells with the function of high collagen expression are prepared and grow from adipose, 1*10<9> or more adipose-derived stem cells can be obtained by culturing 30 mL of adipose-derived cells within 14 days, mass cell quantity required by clinical aesthetic surgery can be met, secondary liposuction can be avoided, and pain caused by secondary liposuction can be relieved. According to the method, the invention further provides a product prepared from the adipose-derived stem cells with the function of high collagen expression. The product can be applied to preparation and growth of the adipose-derived stem cells with the function of high collagen expression.
Owner:岳萍
Bioadhesive vaginal plug for treating vaginitis and cervicitis as well as preparation method of bioadhesive vaginal plug
InactiveCN102600456AEasy to synthesizeGood curative effectPeptide/protein ingredientsSuppositories deliveryPolyvinyl alcoholCervicitis
The invention discloses a bioadhesive vaginal plug for treating vaginitis and cervicitis as well as a preparation method of the bioadhesive vaginal plug. The bioadhesive vaginal plug for treating vaginitis and cervicitis consists of major medicine and a bioadhesive vaginal plug substrate, wherein major ingredients of each vaginal plug comprise 100 to 300mg of total alkali of sophora alopecuroids and 50 to 150IU of external recombinant human basic fibroblast growth factors, the bioadhesive vaginal plug substrate comprises N-succinyl-chitosan, polyving akohol-1788, glycerol, gelatin and injection water, wherein the mass ratio of N- succinyl-chitosan: polyving akohol-1788: glycerol: gelatin: injection water is (1.7-1.9): (1-1.4): (16-20): (18-22): (54.7-63.3), and the total mass of auxiliary materials is 2.65 to 2.9g.
Owner:LANZHOU UNIV SECOND HOSPITAL
Anti-aging skin activating and caring liquid containing growth factors and preparation method thereof
InactiveCN107648066AReduce harmIncrease elasticityCosmetic preparationsToilet preparationsAdditive ingredientLotion
The present invention relates to an anti-aging and revitalizing skin care liquid containing growth factors and a preparation method thereof. 0.5-3 parts, recombinant human epidermal growth factor 0.00001-0.0005 parts, recombinant human basic fibroblast growth factor 0.00001-0.0005 parts, naringenin 0.05-1 part, N-acetylglucosamine 1-4 parts, preservative 0.01-1 part, 4-8 parts of glycerin, 1-3 parts of panthenol triacetate, 2-6 parts of keratan sulfate oligosaccharides and 50-70 parts of water. The composition of the skin care lotion provided by the invention is reasonably matched, scientifically proportioned, exerts a synergistic effect, and has outstanding moisturizing and anti-aging effects.
Owner:GUANGDONG COOWAY BIOTECH CO LTD
Method for producing recombinant human basic fibroblast growth factor from paddy rice seeds
InactiveCN103865932AActivePeptide preparation methodsFibroblast growth factorGenetically modified rice3T3 cells
The invention provides a OsrbFGF gene optimized by paddy rice genetic codon, correlated vectors, a method for preparing OsrbFGF transgenic paddy rice seeds, and a separation purification method of OsrbFGF. The OsrbFGF transgenic paddy rice seed is prepared through specific expression of OsrbFGF by paddy rice endosperm cells, and OsrbFGF is separated and purified. The purity of obtained OsrbFGF is 95%, and obtained OsrbFGF has the activity of promoting NIH / 3T3 cell proliferation in vitro and promoting wound healing in vivo.
Owner:WUHAN HEALTHGEN BIOTECHNOLOGY CORP
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