81 results about "Recombinant human basic fibroblast growth factor" patented technology

The invention discloses a stem cell culture medium. The stem cell culture medium comprises an improved DMEM (Dulbecco's Modified Eagle Medium) / F12 basal culture medium and an additive, wherein the additive comprises sodium bicarbonate, selenium amino acid chelate, recombinant human insulin growth factors, recombinant human basic fibroblast growth factors, recombinant human lactoferrin, ascorbic acid, recombinant human platelet-derived growth factors, recombinant human vascular endothelial cell growth factors, octacosanol, polyvinyl alcohol, polyvinylpyrrolidone and recombinant human epidermal growth factors. According to the stem cell culture medium, the potential of stem cells is not influenced while the stem cells can be proliferated rapidly, the proliferation speed of the stem cells is increased by 3-5 times compared with a common culture medium, and further, the stem cell culture medium can be used for culturing the stem cells of various kinds of tissue and has excellent applicability; the cultured stem cells have high differentiation capability, can be differentiated into multiple functional cells and have very high scientific research and medical application values, culture medium components are exact, the quality is stable, and accordingly, the cultured stem cells are not likely to generate human body rejection reaction after transplanting.

The recombinant human basic fibroblast growth factor capsule includes recombinant human basic fibroblast growth factor freeze dried powder 0.1-100 wt% and medicinal stuffing material 0-99.9 wt%. The preparation process includes dissolving mannitol, human blood albumin and recombinant human basic fibroblast growth factor in distilled water, abacterial filtering, freeze drying to obtain powder, sieving, mixing with stoved and sieved mannitol in certain ratio and capsulizing. The capsule is used in treating gastric ulcer in one completely new path different from traditional gastric ulcer treating medicines.

The invention provides a method for efficiently promoting adipose-derived stem cells to proliferate. The method is characterized in that a cell culture plate or a culture dish coated by gelatin or a cell culture bottle coated by gelatin is adopted to efficiently promote the adipose-derived stem cells to proliferate and grow in fat under combined culture of a recombinant human epithelial growth factor, a recombinant human platelet derived growth factor, a recombinant human basic fibroblast growth factor, insulin, transferrin, fetal calf serum and the like, so that cells from 50mL fat can be cultured to obtain 109 adipose-derived stem cells or more within 15 days, and therefore, great cell population for clinically treating diseases and medical cosmetology can be met, secondary liposuction is avoided and pains caused by liposuction are reduced. According to the method, the invention further provides a kit for efficiently promoting the adipose-derived stem cells to proliferate, and the kit can be used for efficiently promoting the adipose-derived stem cells to proliferate and grow.

The invention discloses a novel cell recovery agent. The novel cell recovery agent comprises the following ingredients: 100 ng of a recombinant human epidermal growth factor, 200 ng of a recombinant human basic fibroblast growth factor, 3000 ng of vitamin D, 2 ml of a recombinant human serum albumin, and 8 ml of an RPMI-1640 culture medium. The novel cell recovery agent has the beneficial effects that (1) the novel cell recovery agent does not contain animal ingredients and is safe and reliable to use; (2) the ingredients are completely clear and definite, and the quality is convenient to control; (3) the novel cell recovery agent is a ready-to-use type, and the operation is more convenient; (4) the applicable cell type is not limited; and (5) better cell viability and thawing rate are achieved when the cell is recovered.

The invention discloses a bioadhesive vaginal plug for treating vaginitis and cervicitis as well as a preparation method of the bioadhesive vaginal plug. The bioadhesive vaginal plug for treating vaginitis and cervicitis consists of major medicine and a bioadhesive vaginal plug substrate, wherein major ingredients of each vaginal plug comprise 100 to 300mg of total alkali of sophora alopecuroids and 50 to 150IU of external recombinant human basic fibroblast growth factors, the bioadhesive vaginal plug substrate comprises N-succinyl-chitosan, polyving akohol-1788, glycerol, gelatin and injection water, wherein the mass ratio of N- succinyl-chitosan: polyving akohol-1788: glycerol: gelatin: injection water is (1.7-1.9): (1-1.4): (16-20): (18-22): (54.7-63.3), and the total mass of auxiliary materials is 2.65 to 2.9g.