Anti-aging skin activating and caring liquid containing growth factors and preparation method thereof
A growth factor and anti-aging technology, applied in medical preparations containing active ingredients, preparations for skin care, pharmaceutical formulas, etc., can solve problems such as high cost and complex preparation process, achieve scientific proportioning, restore lustrous luster , significant skin care effect
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Embodiment 1
[0027] Embodiment 1 Keratan sulfate oligosaccharide preparation and analysis
[0028](1) Take 100g of keratan sulfate, dissolve it in 600ml of 0.1M acetate buffer (pH 6.0), add 50U of mixed enzyme (cutanase Ⅱ and N-acetyl-β-D-glucosaminidase at 1: 1 mass ratio), degraded at 37°C for 24h. After the reaction, 4 times the volume of ethanol was added, stirred, left overnight at room temperature, centrifuged at 3000 rpm for 10 min, and the supernatant (supernatant A) was taken. Add 600ml of distilled water to the precipitate to dissolve, add 6 times the amount of ethanol, stir, leave at room temperature overnight, centrifuge at 3000rpm for 10min, and take the supernatant (supernatant B). Mix supernatant A and supernatant B, concentrate under reduced pressure, use a Bio-Gel-P-2 column (3.6╳134cm) to perform gel filtration with distilled water as a solvent, and freeze-dry the filtrate to obtain a freeze-dried powder.
[0029] (2) The prepared freeze-dried powder is separated by ion...
Embodiment 2
[0032] The preparation of embodiment 2 skin care liquid
[0033] Example 2 The anti-aging and revitalizing skin care liquid containing growth factors comprises the following components and parts by weight: 2 parts of hexapeptide, 1 part of carnosine, 1.5 parts of ectoine, 0.00025 parts of recombinant human epidermal growth factor, recombinant 0.0001 parts of human basic fibroblast growth factor, 0.5 parts of naringenin, 3 parts of N-acetylglucosamine, 1 part of panthenyl triacetate, 5 parts of keratan sulfate oligosaccharides, 1 part of preservative, 6 parts of glycerin 55 parts and 55 parts of water; Wherein, keratan sulfate oligosaccharide is that the keratan sulfate disaccharide and keratan sulfate trisaccharide that embodiment 1 makes form with the mass ratio of 1:0.25.
[0034] Preparation:
[0035] Weigh naringenin, glycerin, ectoine, panthenol triacetate, and keratan sulfate oligosaccharides, add water after mixing, stir and heat to 80°C, after the materials are dissol...
Embodiment 3
[0036] The preparation of embodiment 3 skin care lotions
[0037] Example 3 The anti-aging and revitalizing skin care lotion containing growth factors comprises the following components and parts by weight: 1 part of hexapeptide, 0.5 part of carnosine, 1 part of ectoine, 0.0003 part of recombinant human epidermal growth factor, recombinant 0.0002 parts of human basic fibroblast growth factor, 1 part of naringenin, 3 parts of N-acetylglucosamine, 2 parts of panthenyl triacetate, 4 parts of keratan sulfate oligosaccharides, 1 part of preservative, 8 parts of glycerin 55 parts and 55 parts of water; Wherein, keratan sulfate oligosaccharide is that the keratan sulfate disaccharide and keratan sulfate trisaccharide that embodiment 1 makes form with the mass ratio of 1:0.25.
[0038] The preparation method refers to Example 2.
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