93 results about "Trisaccharide" patented technology

To provide a technique for enhancing the dermal delivery of a composition and, furthermore, enhancing the delivery of the composition to macrophage-surrounding areas.SOLUTION: The present invention provides a liposome with a particle diameter of 300 nm or less, comprising (A) polyglycerin fatty acid ester, (B) phospholipid, and (C) sterol. In a preferred embodiment, the liposome further comprises (D) an amphiphilic molecule having a disaccharide-to-trisaccharide hydrophilic group and an alkyl-chain hydrophobic group within its molecular structure.SELECTED DRAWING: None

The invention discloses application of fucoidin degrading enzyme OUC-MgFucD1 in preparation of fucoidin oligosaccharide, and belongs to the technical field of preparation of the fucoidin oligosaccharide. The amino acid sequence of the fucoidin degrading enzyme OUC-MgFucD1 is as shown in SEQ ID NO. 1, and the fucoidin degrading enzyme OUC-MgFucD1 is as shown The fucose oligosaccharide is fucose, fucodisaccharide and fucotriose, the molecular formula of the fucose is C6H12O8S, the molecular formula of the fucodisaccharide is C12H22O15S2, and the molecular formula of the fucotriose is C18H32O16S. The invention further discloses a preparation method of the fucose oligosaccharide. The fucoidin degrading enzyme OUC-MgFucD1 disclosed by the invention can specifically act on fucoidin, and a specific fucoidin oligosaccharide combination is generated by precisely cutting a main chain glucosidic bond of the fucoidin. The invention provides a key technical support for the development of high-added-value fucoidan oligosaccharide products, and has important significance for promoting the industrial application of the fucoidan oligosaccharide in the fields of functional foods, biological medicines and the like.

This present disclosure provides various diamine centered PEG-saccharide-lipid conjugates. In various embodiments, the conjugates can safely be used as water solubility or bioavailability enhancers.. In one aspect, the present disclosure provides a PEG-saccharide- lipid conjugate having the structural formula (I) wherein m has a number-average value in the range of 2-10; S is a mono-, di- or trisaccharide group, in which each saccharide unit is a sugar, a sugar alcohol, an amino sugar or a sugar acid; L is -C(O)-R1 in which R1 is an alkanyl or alkenyl group having a number-average number of carbons in the range of 6-22, and / or is a steroid acyl group; P is -(CH2-CH2-O)nR2 in which "n" has a number-average value in the range of 5-50 (e.g., 8-45) and R2 is hydrogen and / or alkanyl and has a number average number of carbons in the range 0-4.

The invention discloses a preparation method of chitosan oligosaccharide. The method comprises the following steps: adding chitin into concentrated hydrochloric acid, grinding until the chitin is dissolved into paste, washing with distilled water, and collecting to obtain a white colloidal substance, namely colloidal chitin; dissolving colloidal chitin in a phosphoric acid-citric acid buffer solution to obtain a mixed dissolved solution; adding a crude enzyme solution of composite chitinase into the mixed dissolving solution, placing the mixed dissolving solution in a shaking table for reaction, terminating the reaction in a boiling water bath, centrifuging, and carrying out activated carbon separation, low-temperature rotary evaporation concentration and freeze drying on supernate to obtain a chitosan oligosaccharide finished product. According to the method, chitinase crude enzyme liquid prepared from bacillus subtilis SH21 and chitinase crude enzyme liquid prepared from penicillium oxalicum K10 are used as compound enzymes, chitin is subjected to enzymolysis to prepare the chitosan oligosaccharide, the polymerization degree of the prepared chitosan oligosaccharide is 3-10, trisaccharide, tetrasaccharide, pentasaccharide and hexasaccharide are mainly used, and compared with single enzyme use, the compound enzymes are high in product yield and high in chitin degradation rate.

The invention discloses a method for synergistically degrading chitin by multiple enzymes, which comprises the following steps: adding lysing polysaccharide monooxygenase OsLPMO10A, chitinase and beta-N-acetylglucosaminidase Sohex into an alpha-chitin solution, and carrying out enzymolysis to prepare GlcNAc; the amino acid sequence of the beta-N-acetylglucosaminidase Sohex is as shown in SEQ ID NO. 1 (sequence identifier number 1). The invention further discloses the beta-N-acetylglucosaminidase Sohex and application of the beta-N-acetylglucosaminidase Sohex in degradation of chitin, preparation of GlcNAc and synthesis of lactose-N-trisaccharide II. The invention also discloses a method for synthesizing the lactose-N-trisaccharide II. The beta-N-acetylglucosaminidase Sohex with high hydrolytic activity and promotion effect is excavated, the beta-N-acetylglucosaminidase Sohex is combined with lysing polysaccharide monooxygenase and chitinase to be applied to degradation of alpha-chitin to prepare GlcNAc, and the beta-N-acetylglucosaminidase Sohex has very important environmental protection and industrial significance and has important industrial application value and economic value.

The invention belongs to the technical field of feed, and particularly relates to broiler feed with radix pseudostellariae leftover extract and a preparation method and application of the broiler feed. The radix pseudostellariae root hair extract comprises a polysaccharide-polypeptide compound, polysaccharide is of a net structure formed by a beta-(1-> 3)-D-glucan main chain and a beta-(1-> 6)-glucose branch, and the radix pseudostellariae root hair extract further comprises an alpha-D-GalA-(1-> 2)-beta-D-GlcN-(1-> 4)-beta-D-Gal trisaccharide repeating unit connected to the main chain; the amino acid sequence of the polypeptide is as shown in SEQ ID NO: 1. The preparation method comprises the following steps: performing compound enzyme treatment on radix pseudostellariae root hair, performing gradient alcohol precipitation and purification, and adjusting the radix pseudostellariae root hair extract to enable the content of beta-glucan to be greater than 35%, thereby obtaining the broiler feed. The feed contains a polysaccharide-polypeptide compound, so that the survival rate of the broiler chickens can be increased; according to the preparation method, the extraction rate of effective components of the radix pseudostellariae leftover extract is increased through directional enzymolysis.

The disclosure in one aspect provides a pharmaceutical composition of an antiviral agent, the pharmaceutical composition comprising: the antiviral agent, which can be tenofovir; and a PEG-saccharide-lipid conjugate having the structural formula wherein m has a number-average value in the range of 2-10; S is a mono-, di- or trisaccharide group; L is -C(O)-R1 in which R1 is an alkanyl or alkenyl group having a number- average number of carbons in the range of 6-22, and / or is a steroid acyl group; P is -(CH2-CH2-O)nR2 in which n has a number-average value in the range of 5-50 and R2 is hydrogen and / or alkanyl and has a number average number of carbons in the range 0-4. Such compositions can be provided in a variety of forms. In various such embodiments, the pharmaceutical composition further includes one or more steviol glycosides mainly comprising one or more steviosides and / or rebaudiosides.

The disclosure discloses a chemical synthesis method and an application of V. cholerae serotype O100 O-antigen oligosaccharides, belonging to the field of chemical technologies. The disclosure uses three monosaccharide building blocks and five carboxylic acid derivatives to synthesize five oligosaccharide fragments of V. cholerae serotype O100 O-antigen under the action of the solvent effect, temperature effect, neighboring group participation effect, etc., through orthogonal protection, selective assembly and amide coupling. The absolute configurations and immunological effects of 3,5-dihydroxyhexanoyl in the O-antigen trisaccharide are illustrated by the synthesized oligosaccharide fragments in combination with the NMR analysis and glycan microarray technology, thereby providing a theoretical basis for further structure-activity study and minimal antigenic epitope screening. The disclosure has excellent application prospects in the aspects of development of V. cholerae synthetic glycoconjugate vaccines and new drugs, etc.

The invention relates to the technical field of sugar alcohol preparation, and discloses a preparation method of high-purity crystalline maltitol, which comprises the following steps: firstly, by taking corn starch milk as a raw material, preparing ultrahigh-content maltose syrup with the maltose content of more than or equal to 91%, the wheat triose content of less than or equal to 0.4% and the glucose content of less than or equal to 4% through an enzyme conversion method; carrying out hydrogenation reaction on the syrup by adopting a Raney nickel catalyst, and controlling the hydrogenation side reaction to be 2% or below, so as to obtain hydrogenated liquid with maltitol content of more than or equal to 88%; and then, a pigment separation procedure in a traditional process is omitted, and the hydrogenated liquid is directly subjected to decoloration, ion exchange, evaporation concentration, crystallization, centrifugation and drying treatment to finally prepare crystalline maltitol. According to the method, by screening a specific compound enzyme system and finely regulating the pH value and temperature control environment of a saccharification reaction system, the generation of maltose is effectively promoted, and side reactions such as excessive hydrolysis of glucose and reverse synthesis of trisaccharide are inhibited at the same time, so that the high-purity maltose syrup precursor is obtained.

The invention belongs to the technical field of biology, and particularly relates to Meyerozyma caribba XX2120, and the preservation number of the Meyerozyma caribba XX2120 is GDMCC No: 67777. The invention further relates to a preparation method of the Meyerozyma caribba XX2120. The strain is obtained by taking saccharomycetes X2120 as a starting strain, carrying out ARTP mutagenesis treatment and screening, has relatively strong acid resistance, relatively high salt stress resistance and certain alcohol resistance and aldehyde resistance, can effectively utilize monosaccharide, disaccharide and trisaccharide, has a wide utilization range on carbohydrate and has a relatively good utilization rate on inorganic nitrogen. Moreover, the strain can be used for producing mycoprotein through liquid fermentation by taking Luzhou-flavor distillers' grains as a raw material, and also can be used for performing solid fermentation on the Luzhou-flavor distillers' grains, so that the true protein content of the distillers' grains is increased, the nutritional value of the distillers' grains is improved, and the recycling of distillers' grain resources is effectively promoted.

The invention belongs to the technical field of organic synthesis, and particularly relates to a synthesis method of a Baliella chensinensis lipopolysaccharide O-antigen and application of the Baliella chensinensis lipopolysaccharide O-antigen in the aspect of immunocompetence. The raw materials such as monosaccharide, disaccharide, trisaccharide, tetrasaccharide and the like for preparing the glycoconjugate vaccine can be efficiently synthesized, and the synthesis selectivity and the synthesis efficiency are improved. According to the invention, unprotected ammonia is selected to construct uronic acid amide, and hydrogenation is successfully carried out through the method to obtain the target molecular trisaccharide. Subsequently, monosaccharide, disaccharide and tetrasaccharide target molecules are constructed. Wherein in the construction process of the tetrasaccharide, a method of combining TEMPO and NaOCl is adopted to oxidize the tetrasaccharide into aldehyde, then Pinnick is adopted to oxidize the aldehyde into carboxylic acid, and a two-step one-pot method is adopted to construct the tetrasaccharide. According to the invention, glycoconjugate is prepared by conjugation with HSA, and the glycoconjugate lays a raw material foundation for research and development of the Babyte's fermentum lipopolysaccharide vaccine.

A main object of the present invention is to provide a novel technique for activating T cell immune responses.SOLUTION: Solution to Problem The present inventors have found that the above problems can be solved by a compound represented by the general formula (I): wherein Sugar represents any monosaccharide, disaccharide, or trisaccharide; and R represents a hydrogen atom or an acyl group having 2 to 5 carbon atoms, or a salt thereof.SELECTED DRAWING: None

The invention provides a method for identifying a connection position of a glucosidic bond in an oligosaccharide glucosidic bond isomer, and belongs to the technical field of analysis and detection of saccharide isomers. According to the method for identifying the connection position of the glucosidic bond in the oligosaccharide glucosidic bond isomer, oligosaccharide compounds in a to-be-detected sample are separated at the same time on the basis of a derivatization reagent with a structure as shown in a formula I by utilizing a high performance liquid chromatography-tandem mass spectrometry technology; structural specificity diagnosis ions are generated based on an electron collision excitation collision mode of organic matter ions to identify the types of glycosidic bonds of disaccharide, trisaccharide, tetrasaccharide, pentasaccharide, hexasaccharide and heptasaccharide isomers of different glycosidic bonds. The method is high in identification efficiency and accurate in identification, and can meet the requirements of glycogen storage disease diagnosis biomarker research. And the formula I of the # imgabs0 # is shown in the specification.

The invention discloses beta-N-acetyl hexosaminidase Fmhen (I436S), the amino acid sequence of the beta-N-acetyl hexosaminidase Fmhen (I436S) is shown as SEQ ID NO.5, and the beta-N-acetyl hexosaminidase Fmhen is applied to synthesis of lactose-N-trisaccharide II. The invention also discloses a beta-N-acetyl hexosaminidase Fmhex (W291A), wherein the amino acid sequence of the beta-N-acetyl hexosaminidase Fmhex is shown as SEQ ID NO. 6. The invention also discloses an application of the mutant in synthesis of lactose-N-trisaccharide II. According to the invention, beta-N-acetyl hexosaminidase Fmhex is subjected to mutation modification to obtain two mutants with higher transglycosylation activity, namely Fmhex (W291A) and Fmhex (I436S), the conversion rates of the prepared lactose-N-trisaccharide II are respectively 1.68 times and 1.88 times of those of the beta-N-acetyl hexosaminidase Fmhex, and the beta-N-acetyl hexosaminidase Fmhex has higher application potential. The research of the invention has important industrial production value and economic value.

This invention relates to a composition for improving alcoholic liver damage, belonging to the field of health product technology. This application provides the use of rhodioloside and lactose-N-trisaccharide in the prevention and / or inhibition of alcoholic liver disease. This application also provides a composition of human lactose oligosaccharides and rhodioloside. The compositions described in this application can prevent the occurrence of alcoholic liver disease or inhibit its further deterioration. This application provides a green, safe, and healthy composition, offering an ideal composition for protecting human health.

The invention discloses exo-type alginate lyase VsAly7C as well as a recombinant strain and application of the exo-type alginate lyase VsAly7C. The amino acid sequence of the exo-alginate lyase is as shown in SEQ ID No.4, and the nucleotide sequence for coding the amino acid sequence is as shown in SEQ ID No.1. The suitable reaction temperature of the exo-alginate lyase is 24-28 DEG C, and the exo-alginate lyase has high stability at 0-20 DEG C, so that the exo-alginate lyase can effectively degrade a substrate at low temperature, and belongs to cold-adapted alginate lyase. The exo-type alginate lyase has the capability of degrading alginate, polyG and polyM, the degradation mode is exo-type, and a final degradation product is unsaturated alginate trisaccharide and has NaCl dependence. The invention further constructs a recombinant vector and a recombinant thallus containing the exo-alginate lyase VsAly7C coding gene, and a good foundation can be provided for industrial application and production of unsaturated alginate oligosaccharides.

The invention relates to the technical field of gene engineering, and particularly discloses a chitinase truncated body, a recombinant expression vector thereof, and a construction method and application of engineering bacteria. The chitinase truncated body is Cq181NC, the amino acid sequence of the Cq181NC is as shown in SEQ ID NO: 1, and the nucleotide sequence for coding the chitinase is as shown in SEQ ID NO: 2. According to determination, the chitinase truncation provided by the invention has the advantages that the specific enzyme activity is obviously improved by 1.8 times compared with that of a wild type, the chitinase truncation is high in environmental change tolerance, and the thermal stability of the chitinase truncation is still 1.43 times that of the wild type (incubation is performed for 8 hours at 35 DEG C). More importantly, the spectrum of the enzymolysis product is changed: the wild type is mainly disaccharide and shows a typical excision enzyme mode; and the truncated Cq181NC generates a plurality of oligosaccharides including monosaccharide, disaccharide, trisaccharide and hexasaccharide, shows double catalytic characteristics of endonuclease and exonuclease, and has a better application prospect.

The invention provides a production method for increasing the yield of maltose, which comprises the following steps: proportioning, liquefying and saccharifying, controlling the concentration of starch milk through proportioning, reducing the concentration of syrup, improving the flowability of a system, obtaining a liquefied liquid with a low DE value in the liquefying process, and simultaneously adjusting the pH values of different reaction steps, so that the yield of maltose is increased. Finally, after saccharification is completed, the ultrahigh malt syrup which is low in content of glucose, maltose trisaccharide, maltose trisaccharide or more and high in maltose content is obtained, and the highest maltose content can reach 93%.

The invention relates to a group B streptococcus oligoglycoprotein conjugate as well as a preparation method and application thereof, and belongs to the technical field of group B streptococcus vaccine development. Inactivated group A streptococcus C5a peptidase ScpA193 is used as a carrier and is connected with group B streptococcus oligosaccharide antigens to prepare the group B streptococcus-resistant oligosaccharide protein conjugate, and the oligosaccharide antigens are oligosaccharide trisaccharide derivatives, oligosaccharide pentasaccharide derivatives or oligosaccharide octasaccharide derivatives of group B streptococcus cell wall surface polysaccharides. The oligosaccharide protein conjugate prepared by the invention can simultaneously induce two types of antibodies with different target spots to synergistically aim at group B streptococcus, and is a compound vaccine. The vaccine has better applicability, can more effectively inhibit group B streptococcus, and is not limited by the problem of bacterial drug resistance caused by use of a large amount of antibiotics.

The present invention relates to a method for producing trisaccharides. The method for producing trisaccharides, according to the present invention, provides a high conversion yield of trisaccharides from a substrate and enables mass production of trisaccharides at low cost, and thus provides excellent applicability to food, cosmetics, and pharmaceutical industries. Trisaccharides obtained by the production method of the present invention have low digestibility and low calories, and thus are suitable for industrial use. Novel amylosucrase according to the present invention can be produced at low cost and has excellent reaction activity for trisaccharide production.

The invention relates to a human milk oligosaccharide composition and application of the composition in preventing and / or inhibiting obesity and obesity-related diseases, and belongs to the technical field of human milk oligosaccharide application. The composition contains lactose-N-trisaccharide and other components, and the composition can prevent and / or inhibit obesity and prevent and / or regulate the fat content, cholesterol content, blood sugar and other indexes of a human body. The invention provides a green and healthy composition.

Disclosed is a novel lamellar structure regeneration agent for the stratum corneum. A lamellar structure regeneration agent according to the present invention contains hydrolyzed sericin as an active ingredient. The lamellar structure regeneration agent according to the present invention has the effect of regenerating a lamellar structure of intercellular lipids in the stratum corneum of the skin. The lamellar structure regeneration agent preferably further contains tocopheryl phosphate and / or a trisaccharide. The trisaccharide is preferably raffinose.

This invention discloses an antitumor natural drug composition based on Norway maple and its preparation method, relating to the field of biomedical technology. The compound is selected from at least one of the following structures: 3-O-[β-D-glucopyranosyl-(1→4)-[β-D-galactopyranosyl-(1→2)]]-β-D-glucopyranoside-21-O-acetyl-22-O-angeloyl yrutile alcohol, with the molecular formula C2. 55 H 86 O 23 This invention isolates a class of triterpenoid saponins with a rutinol skeleton from the bark of Norwegian maple, enriching the chemical composition library of maple species. The compounds contain unique oligosaccharide chains and diacyl substitution patterns at C-21 and C-22. One compound has a rare branched acyl group (3-hydroxy-4-methylhexanoyl) at C-21, while the other compound contains an arabinofuranose unit in its C-3 trisaccharide chain. These structural features are rare in natural products and demonstrate excellent structural novelty.

The invention discloses a method for increasing the yield of lactoyl-N-neotetraose based on genome integration and transporter screening, and belongs to the field of microbial genetic engineering. According to the invention, a lactoyl-N-trisaccharide II synthesis module and a lactoyl-N-neotetraose synthesis module are rapidly integrated to different sites of an escherichia coli genome in a multi-copy manner by virtue of a CRISPR (clustered regularly interspaced short palindromic repeats)-associated transsposases gene editing technology, so that the use of plasmids is reduced, and the metabolic burden of cells is reduced. And in a 3L bioreactor, the yield of the lactyl-N-neotetraose reaches 30.56 g / L. Through over-expression of the transporter, the transporter which is most obvious in improving the yield of the lactoyl-N-neotetraose is screened to be YbSET, the yield of the lactoyl-N-neotetraose reaches 54.12 g / L, and excellent industrial application prospects are shown.

Disclosed is a chemical synthesis method for Pseudomonas aeruginosa O5 serotype O-antigen oligosaccharide, which belongs to the field of chemical synthesis. In the disclosure, O-antigen trisaccharide is constructed with a D-glucuronic acid building block and a D-fucosamine building block, where the stereoselective synthesis of a 1,2-α-cis-glycosidic bond of D-fucosamine depends on remote acyl participation and reagent regulation, and synthesis of two types of 1,2-β-trans-glycosidic bonds of 2,3-diaminomannuronic acids is achieved via SN2 nucleophilic substitution of azido at position C2; and via selective assembly of protecting groups, orthogonal modification of modifying groups, and regulation of the reactivity of glycosyl donors and acceptors, multifunctional modified O-antigen target trisaccharide is successfully prepared. According to the method in the disclosure, the raw materials are cheap and easily available, and the preparation method is simple and easy to repeat. Therefore, the method has a very good application prospect in developing vaccines against P. aeruginosa.

The invention is in the technical field of synthetic biology, metabolic engineering and cell culture. The present invention provides cells for the production of one or more biological products, where the cells are capable of producing one or more precursors used in the production of the one or more biological products, and where the cells are genetically engineered to express a sugar input that internalizes at least one of the one or more precursors. The present invention further provides a cell genetically engineered to express or overexpress a sugar input body having an uptake activity with respect to a milk-N-trisaccharide (LN3, GlcNAc-beta 1, 3-Gal-beta 1, 4-Glc). The invention also provides an application of the cell in culture or incubation. Also described are methods for producing one or more biological products by using any of the sugar input bodies and purifying the biological products. The present invention further relates to sugar input bodies having ingestion activity with respect to LN3 and to the use of any one of said sugar input bodies for the production of one or more biological products.

The present invention relates to a method for preparing oligosaccharides by a glycoside phosphorylase combined with monosaccharide kinase and polyphosphate kinase. The method comprises the steps of using donor monosaccharide, acceptor sugar, polyphosphate, and low equivalent adenosine phosphate as substrates, adding magnesium chloride to the substrate, and then co-catalyzing the reaction for 1.5 to 22 hours at 25 to 40 ° C by polyphosphatase, monosaccharide kinase and glycoside phosphorylase to generate crude oligosaccharide products, and obtaining oligosaccharides after purification. The present invention provides a method for preparing important oligosaccharides by using monosaccharides, acceptor substrates, polyphosphate, and low equivalent adenosine phosphate as substrates, and realizing ATP regeneration by using polyphosphate kinase as substrate, effectively solving the problem that a large amount of expensive sugar nucleotide donors and auxiliary factor ATP need to be added in the process of synthesizing oligosaccharides by bioenzymatic method, resulting in the problem that the cost is too high and cannot be mass-produced. The method of the present invention was successfully used to synthesize lactoyl-N-disaccharide, galactoyl-N-disaccharide, chitosan, and protein N-sugar chain core trisaccharide derivatives.

The invention relates to a compound with a triterpenoid saponin structure as well as a preparation method and application thereof, and the structure of the compound with the triterpenoid saponin structure is shown in the following formula (I) or (II): # imgabs0 #, wherein R1 represents glucosyl or methyl; r2 represents a methyl group, a hydroxymethyl group, a glucosyl group or a glucodisaccharide group; r3 represents a glucosyl group or a hydroxymethyl group; r4 represents hydrogen or hydroxyl; and R5 represents a hydroxyl group or a glucose triglycosyl group. According to the invention, active ingredients of pimpinella amygdaliana are extracted, a series of novel compounds with triterpenoid saponin structures are obtained for the first time, and further cell viability verification and function verification prove that the compounds with the triterpenoid saponin structures have no cytotoxicity and can promote differentiation of osteoblasts; animal experiments prove that the compound with the triterpenoid saponin structure can be used for treating osteoporosis, and is clear in effect and safe.

We provide a technology that can activate NRF2. [Solution] An NRF2 activator comprising one or more chondroitin sulfate oligosaccharides selected from (a) to (c) below as an active ingredient: (a) a disaccharide having a chondroitin sulfate structure (a disaccharide of CS), (b) a trisaccharide having two N-acetyl-D-galactosamine residues and a chondroitin sulfate structure (a trisaccharide of CS having two GalNac residues), (c) a tetrasaccharide having a chondroitin sulfate structure (a tetrasaccharide of CS). According to the present invention, NRF2 can be activated. This is expected to promote homeostatic reactions in the body, such as antioxidant reactions, anti-inflammatory reactions, and detoxification reactions, and contribute to maintaining and improving health. It is also expected to contribute to the prevention and improvement of various unhealthy conditions and diseases caused by oxidative stress, inflammation, and toxic chemicals.

Disclosed are a chemical synthesis method for Vibrio cholerae serotype O100 O-antigen oligosaccharide, and a use, belonging to the technical field of chemistry. The present invention uses three monosaccharide building blocks and five kinds of carboxylic acid derivative, and under the effects of solvent, temperature and neighboring group participation, through orthogonal protection, selective assembly and amide coupling, five Vibrio cholerae serotype O100 O-antigen oligosaccharide fragments are synthesized. Using the synthesized oligosaccharide fragments, combined with NMR analysis and carbohydrate chip technology, the absolute configuration and immunological function of the 3,5-dihydroxyhexanoyl group in the O-antigen trisaccharide are clarified, providing a theoretical basis for further structure-activity research and minimal antigen epitope screening. The present invention has promising applications in the synthesis of Vibrio cholerae glycoconjugate vaccines and the development of new drugs.