34 results about "Rhodioloside" patented technology

The invention discloses a co-fermentation method based on sphingomonas and a rhodiola rosea extracting solution and application of a product, and belongs to the technical field of microorganisms. By utilizing a special rich enzyme system of sphingomonas and combining with an early-stage composite enzymolysis process and synergistic effect, the wall breaking efficiency of rhodiola rosea cell walls and the conversion degree of active components of rhodiola rosea are greatly improved, the bottlenecks of low efficiency and low bioavailability of a traditional rhodiola rosea extraction method are successfully overcome, and the rhodiola rosea extraction method is suitable for industrial production. The functional components such as salidroside and flavone can be fully released and converted. Secondly, different from non-symbiotic bacteria (such as lactic acid bacteria and saccharomycetes) which are commonly used for fermentation in the prior art, a potential symbiotic bacterium of sphingomonas XJL-TP07 from a special environment is innovatively selected and has better metabolic suitability with the rhodiola rosea component, the fermentation process is milder and safer (no irritant is produced), and the rhodiola rosea fermentation method is suitable for large-scale production. And a synergistic interaction effect can be generated through a unique biotransformation path.

The invention discloses saccharomyces cerevisiae with high yield of salidroside as well as a construction method and application of the saccharomyces cerevisiae. According to the recombinant saccharomyces cerevisiae, ribulose-5-phosphate isomerase RKI1 and transketolase TKL1 are expressed in host bacteria, so that precursor supply is increased; reverse methyltransferase ARO2 and phenylalanine decarboxylase ARO10 are expressed at the same time, and a tyrosine branch pathway is adjusted; then, UDP-glycosyl transferase of different sources is expressed, and iterative site-specific mutagenesis is carried out on the UDP-glycosyl transferase to enhance conversion of hydroxytyrosol to salidroside; the salidroside production performance of the recombinant strain is verified on the basis of a precursor supply pathway, a tyrosine regulation pathway and key enzyme screening, and the salidroside production capacity of the saccharomyces cerevisiae is further improved.

The invention discloses an oral hard capsule health-care product with an anti-aging function as well as a preparation method and application of the oral hard capsule health-care product. The health-care product is mainly prepared from eight active ingredients including EGT (Ergothioneine), Ca-AKG (alpha-ketoglutarate), PQQ (pyrroloquinoline quinone), fisetin, salidroside, urolithin A, spermidine and an apple stem cell extract. Through scientific compatibility of the various components, the composition disclosed by the invention can comprehensively intervene in a cell aging process through a synergistic effect of a plurality of pathways of enhancing the cell antioxidant capacity, improving the mitochondrial function, activating cell autophagy, promoting cell regeneration and repair and the like. The invention also provides a preferred content range of the health-care product, a preparation method of the health-care product and application of the health-care product in preparation of health-care foods for delaying senescence, improving cell viability or improving mitochondrial functions. The product provided by the invention has the characteristics of multiple targets and synergistic interaction, and provides a new effective scheme for anti-aging intervention.

This invention relates to a composition for improving alcoholic liver damage, belonging to the field of health product technology. This application provides the use of rhodioloside and lactose-N-trisaccharide in the prevention and / or inhibition of alcoholic liver disease. This application also provides a composition of human lactose oligosaccharides and rhodioloside. The compositions described in this application can prevent the occurrence of alcoholic liver disease or inhibit its further deterioration. This application provides a green, safe, and healthy composition, offering an ideal composition for protecting human health.

The application provides a glycosyltransferase and its application in preparing rhodiolin, and relates to the technical field of enzyme engineering. Fraxinus pennsylvanica The glycosyltransferase FpUGT in the application is derived from Malpighia emarginata DC. There is no report about the glycosyltransferase gene capable of directly catalyzing synthesis of rhodiolin in the plants of the genus Malpighia, the application provides a new biological route for searching for the glycosyltransferase with high efficiency in synthesizing rhodiolin, and also provides a new biological material for preparing rhodiolin.

This invention relates to the field of biomedical technology, specifically disclosing an anti-inflammatory and bone-promoting rhodioloside composite hydrogel and its preparation method. The thermosensitive carrier is a triple-response system, which enhances the drug release rate only in a specific microenvironment at 37°C, with a pH of 5.5-6.5 at the site of inflammation, and in which MMPs are overexpressed. MMPs are highly expressed at the implant periodontitis site, and MMP-sensitive peptides can be specifically degraded by this enzyme. This process can trigger the precise release of rhodioloside and quercetin active ingredients in the gel, avoiding systemic drug diffusion that could lead to drug waste or side effects at other sites. Rhodioloside and quercetin synergistically downregulate the expression levels of inflammatory factors such as IL-6 and TNF-α, while nisin and chitosan-ε-polylysine grafts inhibit the growth of Porphyromonas gingivalis. Runx2 / CON expression is increased, achieving a closed-loop treatment of anti-inflammatory, antibacterial, and bone-promoting effects.

This invention proposes a method for preparing dental pulp stem cell exosomes and their applications, belonging to the field of exosome technology. Dental pulp stem cells from exfoliated deciduous teeth are seeded into serum-free α-MEM medium containing microparticles, insulin-like growth factor, transforming growth factor-β1, penicillin, and streptomycin for culture. Exosomes are isolated, dispersed in PBS solution, and incubated with microparticles, propylene glycol, and rhodioloside to obtain highly active dental pulp stem cell exosomes. The surface of these exosomes is then modified with shikonin and interferon to obtain dental pulp stem cell exosomes. The dental pulp stem cell exosomes prepared by this invention exhibit good affinity for titanium implant-mediated osteointegration repair materials, demonstrating good anti-inflammatory, immunomodulatory, osteogenic, and damage-reducing effects. Furthermore, the preparation method is simple, yields high output, and operates under mild conditions, showing broad application prospects.

The application discloses a night work activating composition containing multiple plant extract, which comprises the following components in mass: 3-5 parts of extract A, 10-15 parts of extract B, 2-4 parts of extract C, 1.5-3 parts of curcumin nano-liposome, 2-5 parts of lactobacillus fermentation filtrate, 1-3 parts of lock water magnetite, 0.1-0.3 parts of xanthan gum / acrylate compound thickening agent and 0.5-1 part of preservative composition. The night work activating composition containing multiple plant extract of the application is compounded by extract A with methyl jasmonate as a target component, extract B with rhodioside and syringic acid as target components and curcumin nano-liposome, compared with the same kind of products on the market, the antioxidant and anti-glycation capacity of the night work activating composition can be targetedly strengthened, the transdermal efficiency is improved, and the DNA repair effect and anti-inflammatory effect are improved.

The invention discloses an aquatic product liver protection feed additive based on a multi-target synergistic effect as well as a preparation method and application thereof, and belongs to the technical field of aquatic product feed additives. According to the invention, betaine hydrochloride, bile acid, taurine, macleaya cordata extract, ligustrum lucidum polysaccharide, oleanolic acid, glucurolactone, salidroside, vitamin E and nano-selenium are used as raw materials to prepare the aquatic product feed additive capable of protecting the liver, and the ligustrum lucidum polysaccharide and the salidroside play a role in protecting the liver; bile acid, taurine and betaine hydrochloride are used for promoting fat metabolism, vitamin E and nano-selenium play an anti-oxidation role, glucurolactone plays a detoxification role, oleanolic acid and macleaya cordata extract play an anti-inflammatory role, and vitamin E and nano-selenium play an anti-inflammatory role. The prepared liver-protecting feed additive for the aquatic products can cope with abnormal conditions such as hepatomegaly, white liver and green liver caused by excessive feeding, metamorphic feed feeding, water quality deterioration, pathogenic bacteria infection and the like.

The application provides a blood-activating and stasis-removing medicinal composition containing fermented douchi and a preparation method thereof, belongs to the technical field of biological medicine, and the medicinal composition comprises fermented douchi, drynaria extract, extract of pereskia aculeata, tanshinone II A, notoginsenoside R1, ligustrazine, rhodioside, total flavones of hawthorn leaves and total flavones of mulberry leaves. The fermented douchi is prepared from soybeans and black beans through fermentation by bacillus subtilis and bacillus coagulans. The drynaria extract is prepared from drynaria powder through common enzymolysis by cellulase, chitinase and beta-glucanase, extraction by a two-phase solvent, and purification by a dextran gel column. The extract of pereskia aculeata is prepared from pereskia aculeata powder through common enzymolysis by pectinase, cellulase and xylanase, common enzymolysis by bromelain and subtilisin, and purification by a macroporous adsorption resin column. The medicinal components are prepared in a specific proportion to relieve blood stasis from two dimensions of "improving blood flow dynamics + preventing thrombus formation".

The present application relates to the field of bioengineering, and particularly relates to genome, expression module, recombinant strain and application thereof in preparation of rhodiolin. The present application provides a genome comprising one or more of expression gene, knockout gene, overexpression gene and inhibition gene. The present application constructs a strain capable of utilizing sugarcane molasses as the only carbon source to generate rhodiolin, thereby reducing the cost of materials; then the metabolic network of the microorganism is restructured through metabolic engineering, the metabolic flow is optimized, the metabolic flux among different metabolic modules is reasonably distributed, and the efficient synthesis of the target product is promoted; finally, the mining and screening of enzymes are crucial, the enzymes with higher activity and higher specificity for specific substrates are mined and screened based on the combination of the trained large language model and the machine learning model and the structural information of the substrates, which significantly improves the titer and conversion rate of rhodiolin generation, and at the same time, reduces the generation of by-products and the accumulation of precursor substances, and will promote the large-scale production and application of rhodiolin.

The application provides a rhodioloside production strain and a construction method and application thereof, the strain lacks tyrR, feaB, ldhA, ushA, pykF genes, up-regulates tktA , aroG fbr 、 tyrA fbr、 pgm, galU, gntK the transcription level of a gene, and heterologously introduces a TDC gene from Lactobacillus brevis, a TYO gene from Micrococcus luteus, a ARO10 gene from Saccharomyces cerevisiae, and a ADH6 gene, heterologously introduces a GDH gene from Bacillus and a sugar transferase Oft8gt1 gene from Osmanthus fragrans; the construction method is to reconstruct the metabolic network of Escherichia coli to rhodioloside through metabolic engineering and genetic engineering means, which can effectively utilize glucose to synthesize rhodioloside from scratch, improve the production level of rhodioloside, and lay a solid foundation for the industrialized scale production thereof.

The invention provides a new application of salidroside in delaying replicative senescence of bone marrow mesenchymal stem cells. Research finds that the salidroside can delay replicative senescence of the bone marrow mesenchymal stem cells by reducing protein expression levels of p53, p21 and p16 of the bone marrow mesenchymal stem cells; the salidroside can delay the replicative senescence of the bone marrow mesenchymal stem cells by reducing the mRNA levels of p53, p21 and p16 of the bone marrow mesenchymal stem cells; the salidroside can reduce the positive rate of aging-related beta-galactosidase staining of the bone marrow mesenchymal stem cells. The application of the traditional Chinese medicine rhodiola rosea in the field of stem cell aging resistance is expected to be expanded, important guarantee is provided for in-vitro amplification, stemness maintenance and subsequent application of bone marrow mesenchymal stem cells, and the application has important economic value and social significance.

This invention discloses an endophytic fungus of *Aspergillus sp.* and its applications. The invention provides a novel endophytic fungus, PS4, which possesses unique antibacterial and antitumor activities. The secondary metabolites produced during fermentation contain various active ingredients, such as shikonin, 10-hydroxycamptothecin, and rhodioloside, exhibiting significant antioxidant, antibacterial, and antitumor effects. Simultaneously, the controllable fermentation process of the endophytic fungus improves the stability and yield of the product. The use of bio-fermentation is more environmentally friendly, reducing environmental impact, and ultimately provides a new option for clinical treatment, especially in combating drug-resistant strains and antitumor drugs.

The invention relates to the technical field of biology, and particularly discloses a strain of genetic engineering recombinant escherichia coli (Escherichia coli) and an application of the Escherichia coli in production of salidroside and tyrosol. The recombinant escherichia coli is obtained by overexpressing exogenous ketonic acid decarboxylase, glycosyltransferase and endogenous UDP-glucose synthesis route key enzyme in cells of an L-tyrosine production strain. The strain can be used for efficiently synthesizing salidroside from the beginning through aerobic fermentation by taking glucose as a substrate, and antibiotics and inducers do not need to be added in the fermentation process. The invention further provides a production method of the tyrosol, high-concentration salidroside is efficiently hydrolyzed to generate the tyrosol by using a provided acid hydrolysis process, and the conversion rate reaches 98%. The genetic engineering recombinant escherichia coli provided by the invention can be used for flexibly and selectively deciding to produce salidroside or tyrosol, and the production process is simple and efficient, and can be better applied to industrial production.

The invention discloses a preparation method of a traditional Chinese medicine preparation with an anti-fatigue effect in the technical field of traditional Chinese medicine preparations, the traditional Chinese medicine preparation is an atomized preparation, the atomized preparation takes an anti-fatigue nanoparticle suspension as a mother solution, a function optimizing agent is added for formula improvement, and the function optimizing agent comprises a dispersing agent, a stabilizing agent and a mucous membrane protector. A pullulan carrier is prepared by adopting a bromination-amination-oxidation three-step modification process, a compact three-dimensional network structure with hydrophobic-hydrophilic characteristics is constructed by combining hydroformylated lysine crosslinking and cinnamyl aldehyde grafting modification, the stable carrier structure is obtained, and through compounding of ganoderma triterpenes, salidroside and ginseng total saponins and combination of the synergistic effect of cinnamyl aldehyde, the activity of ganoderma triterpenes, salidroside and ginseng total saponins is improved, and the activity of ganoderma triterpenes, salidroside and ginseng total saponins is improved. The functions of multiple components are complementary, and the anti-fatigue effect is remarkably enhanced.

This invention relates to the field of health food technology, and more particularly to an anti-aging hard capsule of *Polygonum multiflorum* peptide and traditional Chinese medicine compound, and its preparation method. By mass percentage, it comprises the following components: 40%–60% *Polygonum multiflorum* small molecule protein peptide freeze-dried powder, 10%–20% Astragalus polysaccharide extract, 5%–15% Polygonatum polysaccharide extract, 2%–8% Lycium barbarum polysaccharide extract, 1%–5% Panax notoginseng saponin extract, and 3%–10% auxiliary traditional Chinese medicine components and pharmaceutically acceptable excipients. The auxiliary traditional Chinese medicine components are Rhodiola rosea glycoside extract and Echinacea polyphenol extract or licorice powder. This invention's compound follows the principles of traditional Chinese medicine formulation, and the product is rich in small molecule peptides and various active ingredients. It can promote collagen synthesis, clear senescent cells, enhance immunity, resist oxidation, regulate intestinal flora, and improve microcirculation, exhibiting significant anti-aging and anti-fatigue effects. The preparation process is scientifically controllable and has wide applicability.

The invention belongs to the technical field of biology, and particularly discloses application of glycosyl transferase PdUGT from apricot trees in biosynthesis of salidroside, and the sequence of the glycosyl transferase PdUGT is as shown in SEQ ID NO. 2. The gene coding SEQ ID NO.2 is transferred into bacillus licheniformis, tyrosol is added into a fermentation culture medium for fermentation, biologically fermented salidroside can be obtained, tyrosol can be efficiently and specifically converted to synthesize salidroside, 12 g / L of tyrosol can be converted to 23.92 g / L at most, and the conversion rate is larger than 92%. The invention lays a foundation for industrial production of salidroside by a microbial conversion method.

This invention provides a method and culture medium for producing rhodioloside from adventitious roots of Rhodiola rosea via suspension culture. The method employs a segmented liquid suspension culture technique, comprising: establishing a sterile seedling system for Rhodiola rosea, obtaining sterile seedlings through specific sterilization and culture conditions; liquid culture and acclimatization of adventitious roots, shortening the growth cycle to 35-40 days and improving growth vigor by utilizing optimized culture media and acclimatization strategies; and optimizing the culture for efficient accumulation of rhodioloside, transferring adventitious roots to a specific induction medium and culturing them on a shaker for 8-10 days, ultimately achieving a rhodioloside content ≥0.6%. This invention eliminates the need for natural root collection, avoiding damage to wild Rhodiola rosea resources. It features a short production cycle, high efficiency, stable quality, and no heavy metal or pesticide residues, enabling large-scale industrial production to meet the demands of the pharmaceutical and health product markets. Simultaneously, it reduces reliance on wild resources, contributing to the sustainable development and utilization of endangered traditional Chinese medicine.

This invention proposes an acid-loving glycosyltransferase mutant with a mutation at position 415 and its application in the production of rhodioloside, belonging to the field of bioengineering technology. It solves the problem of low synthesis efficiency of rhodioloside under acidic conditions. It comprises an acid-loving glycosyltransferase mutant obtained by mutating any one of the amino acids shown in SEQ ID NO.2 at position 415. It is mainly used for high-yield production of rhodioloside under acidic conditions.

The present application provides transferase and its application in preparing rhodiolin, and relates to the technical field of enzyme engineering. The present application discloses UGT30, UGT50 and UGT55 proteins for the first time, and finds that the proteins have glycosyltransferase function, can be used as glycosyltransferase to efficiently catalyze substrate to synthesize rhodiolin, and provide new bioactive material for preparing rhodiolin. The present application also provides engineering bacteria expressing the glycosyltransferase, and a method for preparing rhodiolin by using the glycosyltransferase or the engineering bacteria, the method is simple and easy to operate, the yield and conversion rate of rhodiolin are high, and the method has good prospect in industrial production of rhodiolin.

This invention belongs to the field of biomedical technology and discloses the application and verification method of rhodioloside in the preparation of drugs for diseases with abnormally elevated NAMPT. Rhodioloside can downregulate the abnormally elevated NAMPT protein expression under pathological conditions and restore NAD in the brain. + This invention aims to restore metabolic homeostasis by blocking the NAMPT-mediated inflammatory cascade upstream, thereby inhibiting microglial overactivation and the release of pro-inflammatory factors TNF-α and IL-1β. The invention also identifies its core upstream target, NAMPT / NAD. + This approach targets a precise population of beneficiaries while overcoming the drawbacks of existing NAMPT inhibitors, which have significant toxic side effects. It combines efficacy and safety, and has high potential for clinical translation.

The invention discloses tyrosol-tolerant escherichia coli and application thereof in salidroside biosynthesis, and belongs to the technical field of genetic engineering. The 5A02 strain obtained by the invention can tolerate 40 mM tyrosol concentration, and has high tyrosol tolerance compared with an original strain 5A. The 5A02 / pkk223-3-ugt85a1 strain obtained by the invention can be used for efficiently converting tyrosol to produce the salidroside. When the strain is subjected to shake flask fermentation, the salidroside yield reaches 5.93 g / L, the large-scale fermentation yield of a 50 L fermentation tank is further increased to 24.40 g / L, and complete conversion of tyrosol is realized. The salidroside synthesis system of'tolerant strain construction + exogenous supplement of the precursor ', established by the invention, gives consideration to the synergistic advantages of de novo synthesis and precursor supply, provides a stable technical scheme for large-scale industrial production of salidroside, and has a wide industrialization prospect.

The invention discloses salidroside nano-stabilized particles and a preparation method thereof, and belongs to the technical field of medical biology. The salidroside nano-stabilized particle disclosed by the invention is prepared from WPI, MD and SA, the salidroside nano-stabilized particles are WPI-MD-SA (White Petroleum Institute-Methyl Diisocyanate-Sulfonic Acid The WPI-MD-SA composite carrier is of a ternary composite nanostructure formed by self-assembly of WPI, MD and SA through hydrogen bonds, electrostatic interaction or hydrophobic interaction; the protein content of the WPI is not less than 85%; the polymerization degree of the MD is 6 to 9; the molecular weight of the SA is 3 * g / mol to 5 * g / mol. After the prepared salidroside nano-stabilized particles are redissolved, the particle size is 200-500 nm, the encapsulation efficiency is not less than 80%, the in-vitro stomach digestion retention rate is not less than 80%, the intestinal tract release rate is not less than 80%, the problems that salidroside is low in bioavailability and poor in environmental stability are solved, and a new way is provided for nanocrystallization application of natural active ingredients.

This invention discloses a verbascoside isoflavone composition and its application in anti-hepatocellular carcinoma and immune remodeling, relating to the field of natural drug formulation technology. The active ingredient of this composition consists of verbascoside, rhodioloside, and taraxasterol in a mass ratio of 42:37:11. The pharmaceutical excipients of the composition consist of pharmaceutical trehalose, sulfobutyl-β-cyclodextrin, and hydroxypropyl-β-cyclodextrin in a mass ratio of 0.4:0.5:0.9. The mass ratio of the pharmaceutical excipients to the total active ingredient is 1.8:1. This invention constructs a multi-target active system through the precise compounding of three plant monomers, synergistically inhibiting hepatocellular carcinoma and regulating immunity. Low-temperature purification using a composite enzymatic hydrolysis coupled with macroporous resin ensures strict purity control and improves bioavailability. A multi-cyclodextrin complex with trehalose is used to construct an inclusion carrier, which improves water solubility and stability through stepwise encapsulation. Gradient freeze-drying is then used to form a powder for injection, which is rapidly and clearly reconstituted. The simplified excipient ratio reduces the metabolic burden on the liver and is suitable for the injection administration needs of hepatocellular carcinoma patients.

The present disclosure relates to a yeast broth of salidroside for animal husbandry applications, animal feed formulations comprising the yeast broth of salidroside, and methods of forming and administering the yeast broth of salidroside.

The invention discloses an extraction method for a rhodiola rosea extract, and relates to the technical field of traditional Chinese medicine extraction. The extraction method for the rhodiola rosea extract comprises the following steps: carrying out steam explosion and composite enzymatic hydrolysis pretreatment on a rhodiola rosea raw material, and then carrying out gradient reflux extraction by using a 70% ethanol solution; carrying out low-temperature cold precipitation treatment on the extracting solution at 0-4 DEG C, and carrying out filter pressing in combination with a filter aid to obtain clear filtrate; and concentrating the filtrate under reduced pressure until the specific gravity is 1.04-1.06, immediately introducing high-shear homogenization treatment, and finally performing spray drying. The core of the invention lies in that a specific gravity 1.05 node coupling homogenization technology is utilized, so that the rheological property of the feed liquid is obviously improved, and the industrial problems that the rhodiola rosea extract is easy to stick to the wall and block a gun during spray drying are thoroughly solved. Meanwhile, low-temperature cold precipitation is adopted to replace traditional macroporous resin purification, ineffective viscous impurities are effectively removed, and meanwhile, full-spectrum active ingredients such as salidroside, polysaccharide and flavone are reserved to the maximum extent.

The application discloses a kind of hierarchical dandelion pollen and astragalus polysaccharide hydrogel integrated patch and its preparation method and application, first to dandelion pollen is defatted and acidolysis treatment, obtain the porous pollen particle with drug loading capacity;Subsequently, porous pollen particles are immersed in rhodioside solution, and rhodioside is loaded onto porous pollen particles by physical adsorption, to obtain drug-loaded pollen;Prepared into raw and cooked paste mixed matrix with kudzu starch, then add prepared drug-loaded pollen and astragalus polysaccharide, after being mixed sufficiently, pre-gel solution is obtained;Finally, pre-gel solution is perfused into patch mold, and after heating and cooling solidification, demoulding is carried out, and the patch is obtained.The application realizes the antibacterial effect of astragalus polysaccharide by the rapid degradation of starch-astragalus polysaccharide matrix, and drug-loaded pollen microcarrier gradually releases rhodioside in wound microenvironment, to achieve long-acting active oxygen scavenging and anti-inflammatory effect.

This invention discloses the application of baicalin and rhodioloside in the treatment of photodermatitis. This invention is the first to discover that the combination of baicalin and rhodioloside has a synergistic effect on the treatment of photodermatitis. This invention provides a new and effective drug combination strategy for the treatment of photodermatitis, and provides new ideas for the research and development of anti-photodermatitis drugs, with good application value and industrial transformation prospects.

The invention discloses a preparation method of a Sijunzi decoction and application of the Sijunzi decoction in cerebral apoplexy repair, and relates to the technical field of traditional Chinese medicine, and the technical key points are as follows: four traditional Chinese medicine monomers, namely astragaloside, salidroside, scutellarin and panax notoginseng saponins, are combined according to a certain proportion to form a novel Sijunzi decoction compound preparation. The Sijunzi decoction compound preparation can be used for treating acute stage and recovery stage of cerebral apoplexy.