The invention discloses a dental pulp
stem cell preparation method. The dental pulp
stem cell preparation method includes the following steps of: transporting dental pulp, wherein a
deciduous tooth isplaced in a sampling
bottle, the sampling
bottle and a medical ice bag are placed back into a transportation box to be transported back to a laboratory; separating
dental pulp stem cells, wherein
tweezers are adopted to clamp the crown of the tooth, then, a
syringe is used for sucking up normal
saline to blow a
dental pulp cavity inwards from a fracture surface of the root of the tooth to wash and loosen the dental pulp, and using a broach for taking out the dental pulp; and proliferating and culturing the
dental pulp stem cells, wherein a complete culture medium is adopted, a dental pulp precipitate is resuspended and transferred to a 12-well microplate, one broach is used for fixing the dental pulp, barbs on another broach are used for stripping the dental pulp to make the dental pulp become fine pieces, the cells in the dental pulp are released, and then culture is conducted. According to the dental pulp
stem cell preparation method, the broach is adopted to strip the
agglomerate dental pulp to release the stem cells in the dental pulp, damage caused by using
collagenase and the like on the
dental pulp stem cells is avoided, damage on the dental pulp stem cells during digestioncan be eliminated, therefore, the extraction success rate of the dental pulp stem cells reaches 100%, and application is simple and convenient.