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38 results about "Symbiotic bacterium" patented technology

Symbiotic bacteria is the one which forms association with either plant , animal or fungus in which bacteria as well as the host , both are benefited. example - Rhizobium bacteria forms association with the root nodules of higher plants , plant provide shelter to the bacteria and bacteria helps in nitrogen fixation.

Method for treating sludge digestive liquid by coupling anaerobic ammonia oxidation and anaerobic methane oxidation

The invention provides a method for treating sludge digestive liquid by coupling anaerobic ammonia oxidation and anaerobic methane oxidation, and belongs to the technical field of wastewater treatment. The method comprises the steps that anaerobic digested sludge is inoculated into an anaerobic methanogenic reactor, partial nitrification sludge is inoculated into a partial nitrification reactor, and anaerobic ammonia oxidation and denitrification-type methane anaerobic oxidation bacterium symbiotic bacterium group sludge is inoculated into an anaerobic ammonia oxidation coupled anaerobic methane oxidation reactor; firstly the sludge digestive liquid enters the anaerobic methanogenic reactor, after water quality of yielding water is adjusted by a middle water tank, the yielding water enters the partial nitrification reactor, and the nitrosation process is realized by controlling aeration time; finally the water quality of yielding water is adjusted by the middle water tank, the yielding water enters the anaerobic ammonia oxidation coupled anaerobic methane oxidation reactor, meanwhile, methane generated by the anaerobic methanogenic reactor is used for aeration, and the autotrophic nitrogen removal process is completed with the help of anaerobic ammonia oxidation bacteria and anaerobic methane oxidation bacteria. The method can be used for solving the problems of insufficient organic carbon source, relatively low C / N ratio, release of greenhouse gases and the like existing in a sludge digestive liquid nitrogen removal treatment process.
Owner:BEIJING UNIV OF TECH

Wrinkle removal composition with symbiotic bacterium combined ferment substance, wrinkle removal eye cream and preparation method of wrinkle removal eye cream

The invention provides a winkle removal composition with a symbiotic bacterium combined ferment substance, a wrinkle removal eye cream and a preparation method of the wrinkle removal eye cream. The wrinkle removal composition comprises the following components in parts by weight: 0.01-5 parts of acetyl hexapeptide-8, 0.1-10 parts of oligopeptide-4, 0.1-10 parts of a crithmum maritimum callus culture filtrate, 0.1-10 parts of dipeptide diamido-butyryl benzyl amide diacetate, 0.01-5 parts of a camellia sinensis leaf extract, 0.1-10 parts of a coffea arabica extract, 0.1-10 parts of a retinol type compound and 1-10 parts of a symbiotic bacterium combined ferment substance. The winkle removal composition is capable of not only promoting c cell proliferation and regeneration and improving skinelasticity, but also effectively preventing oxidation and preventing collagen decomposition. The wrinkle removal eye cream is not only capable of removing dynamic wrinkles, static wrinkles and dry wrinkles which are already formed around eyes, and preventing formation of wrinkles, but also providing long-lasting moisture retention, and making skin tough and healthy.
Owner:广州一一生物技术有限公司

Bacteria strain (KG strain) for culturing bait nematode and nematode culture method

The invention discloses a bacteria strain (KG product system) to culture bait wire insect and culturing method, which comprises the following steps: choosing Xenorhabdus poinarii KG CCTCC M 206091 bacterial strain as producing bacterium; culturing the bacterial strain on protease leather water culture medium at 25-28 deg.c; getting bacterial liquid; adding into wire insect culture medium; switching in germ-free Panagrellus redivivus wire insect; placing under 25-28 deg.c; culturing 6-15 days; getting the culturing wire insect. This P.redivivus wire insect is safe for environment, human and animal, which is good biological bite of aquaculture.
Owner:GUANGDONG ENTOMOLOGICAL INST

Dendrobium officinale growing method

The invention relates to the technical field of plant growing, and provides a Dendrobium officinale growing method. The Dendrobium officinale growing method comprises the following steps: A, selection of a culture host: in a high mountain broadleaf forest, selecting an evergreen tree woodland with thick trunks, abundant moisture, lush tree crowns, loose barks and longitudinal fissures; B, preparation of seedlings: in March to April, selecting the seedlings of one-year-old to two-year-old Dendrobium officinale, cutting out overlong fibrous roots and old stems which are 3 or more years old, and cutting the plants into smaller ones; C, culture pretreatment: performing treatment with a cutter at recesses of the trunks or branches or every other 30-50 centimeters to obtain culture regions, cultivating mosses, and spraying a symbiotic bacterium solution; D, culture: after the proliferation of the mosses, implanting the base parts of the Dendrobium officinale seedlings in the fissures, and performing fixing; E, nursing: after growing, performing root irrigation, performing spraying, and applying a slow-release granular fertilizer and a foliar fertilizer; and F, collection: 1 year after growing, performing collection and pruning. The Dendrobium officinale growing method provided by the invention can solve the problem that the Dendrobium officinale is low in survival rate and poor in quality.
Owner:杭州千岛湖峰川生物科技有限公司

Biological preservation technique for natural latex

The invention discloses a biological preservation technique for natural latex. The biological preservation technique comprises culture of symbiotic bacterium groups for preservation, mixing of a bacterium solution and latex and preservation under light and is characterized in that the symbiotic bacterium groups for preservation are self-supporting symbiotic bacterium groups, the microbiota for preserving the natural latex can better realize internal self-supporting of nutrition required for breeding and do not need to obtain required nutrients from the fresh latex, the fresh latex contains a large amount of water which accounts for 55%-75% the total mass of the latex, the large amount of water can effectively provide a living environment and a metabolite conversion environment for survival of beneficial microorganisms, protein in the fresh latex cannot be easily decomposed and enables the fresh latex to solidify quickly, so that other unnecessary bacteria can be dispelled through breeding of the beneficial microorganisms in the latex, the pH value of the latex is effectively adjusted, a hydrated shell is protected, and high-quality latex capable of being stored for a long time can be obtained.
Owner:昆明珩森科技有限公司

Compounds with tumor cell proliferation resistance, and preparation method and application thereof

The invention belongs to the technical field of medicine, and provides activities and structure of new indole alkaloid compounds 1-10 derived from insect symbiotic bacterium Chaetomium globosum secondary metabolite and also provides a separation purification preparation method and related activities of compounds pillworm chaetoglobosins E and H with favorable antitumor activity. The structural formula is disclosed as (I). The invention provides an alternative compound for developing new antitumor drugs, and has very important meanings for development and utilization of medicinal insect symbiotic bacterium resources.
Owner:HUAZHONG UNIV OF SCI & TECH

Skin conditioning agent compound containing symbiotic bacterium combined fermented substances, preparation method of skin conditioning agent compound containing symbiotic bacterium combined fermented substances, and genifique youth activating concentrate and preparation method thereof

The invention provides a skin conditioning agent compound containing symbiotic bacterium combined fermented substances, a preparation method of the skin conditioning agent compound containing symbiotic bacterium combined fermented substances, and a genifique youth activating concentrate and a preparation method thereof. The skin conditioning agent compound comprises 15-40% of symbiotic bacterium combined fermented substances, 10-20% of white truffle extracts, 30-50% of rose flower water, 5-10% of saccharide isomers and 5-10% of ceramide-3, wherein the total mass of the skin conditioning agentcompound is 100%. The genifique youth activating concentrate provided by the invention can stimulate the regenerative capacity of cells and increase skin elasticity, has the effects of preventing ageing and resisting senescence, besides, can repair skin barriers and increase immunity and tolerance of skin, and has favorable moisturizing efficacy. The skin conditioning agent compound provided by the invention can repair the skin barriers in a comprehensive and multidimensional manner, can increase skin elasticity and is good in moisturizing effects.
Owner:广州一一生物技术有限公司

Sowbug symbiotic bacterium of chaetomium globosum and application thereof to preparation of antitumor compounds

The invention belongs to the technical field of medicine, and relates to a sowbug symbiotic bacterium of chaetomium globosum and application thereof to the preparation of indole alkaloid compounds with the antitumor effects. The invention provides activity and structures of new indole alkaloid compounds 1 to 10 from the secondary metabolites of the chaetomium globosum, and provides a separation purification preparation method and structure identification of the new compounds. The chaetomium globosum provided by the invention is preserved in CCTCC (China Center for Type Culture Collection), andhas the strain preservation number being CCCC M2016343. The preservation time is June 23, 2016; the preservation address is Wuhan University, Wuhan, China. The invention provides backup compounds fordeveloping the novel anti-tumor medicine, and has the important significance on the development and utilization of medical insect symbiotic bacterium resources.
Owner:HUAZHONG UNIV OF SCI & TECH

Group of bemisia tabaci prevention and control target genes and combined application thereof

The invention relates to a group of bemisia tabaci prevention and control target genes and combined application thereof. The group of bemisia tabaci prevention and control target genes comprise a BtGlu gene as shown in SEQ ID NO.1, a BtArgH gene as shown in SEQ ID NO.2 and a BtTY gene as shown in SEQ ID NO.3. The research thought of taking a sugar transporter gene, a basic group and a amino acid synthetic gene as the bemisia tabaci prevention and control target genes is provided for the first time; and experiments of the survival rate of the adult bemisia tabaci, the egg laying rate of the female bemisia tabaci, the influence on symbiotic bacteria of the female bemisia tabaci, the influence on the virus acquiring capacity of the female bemisia tabaci TYLCV and the like verify that the silent expression can obtain a short-term prevention and control effect-reduction of the survival rate of the bemisia tabaci and a long-term prevention and control effect-reduction of the reproductive capacity of the bemisia tabaci.
Owner:天津市农业科学院

DsRNA of gene of symbiotic bacterium of aphids and application thereof to inhibition of growth of aphids

ActiveCN104109671AImprove aphid resistanceBiocideAnimal repellantsBiotechnologyXenorhabdus sp.
The invention discloses dsRNA of a gene of a symbiotic bacterium of aphids and an application thereof to inhibition of growth of aphids. DsRNA provided by the invention is double-stranded RNA formed by a nucleotide shown in a sequence 2 in a sequence table and a nucleotide shown in an inverse complementary sequence of the sequence 2. Experiments prove that the gene of the endotrophic symbiotic bacterium hamiltonella defensa 28469 of wheat aphids is obtained; the gene of the endotrophic symbiotic bacterium hamiltonella defensa 28469 of wheat aphids can be inhibited or silenced, impaired growth and development of the wheat aphids can be caused and lethal effects can be generated by adopting the method of feeding dsRNA in vitro.
Owner:INST OF CROP SCI CHINESE ACAD OF AGRI SCI

Use of fungicide in prevention and control of brown planthopper filial generation

The invention relates to the technical field of biological prevention and control and especially relates to a use of a fungicide in prevention and control of brown planthopper filial generation. The fungicide which has toyocamycin, tetramycin P, tetrin B and tetramycin A total content of 27% is used for prevention and control of brown planthopper filial generation, wherein toyocamycin content is 5.58% by mass, tetramycin P content is 7.09% by mass, tetrin B content is 4.44% by mass and tetramycin A content is 9.64% by mass. The fungicide comprising toyocamycin, tetramycin P, tetrin B and tetramycin A can prevent or inhibit yeast-like symbiote entrance into ovarian tissue from female brown planthopper adult hemolymph thereby influencing a brown planthopper reproductive system, reducing egg laying amount and substantially reducing egg hatchability because of symbiotic bacterium loss so that brown planthopper filial generation is effectively controlled.
Owner:CHINA JILIANG UNIV

Entomopathogenic nematode symbiotic bacterium with broad-spectrum antibacterial function and application thereof

The invention relates to an entomopathogenic nematode symbiotic bacterium XBD102 (Xenorhabdus budapestensis) strain with a broad-spectrum antibacterial function, and the entomopathogenic nematode symbiotic bacterium XBD102 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No: 22057. A fermentation supernatant of the XBD102 strain has a growth inhibition effect on various fungi and bacteria, and can be used for development and application of novel biological pesticide.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI

Rna-based therapeutic methods to protect animals against pathogenic bacteria and / or promote beneficial effects of symbiotic and commensal bacteria

The invention relates to a method to inhibit gene expression in bacteria, which is referred to here as Antibacterial Gene Silencing (AGS). In particular embodiments, the method is used to protect plants and animals against pathogenic bacteria by targeting pathogenicity factors and / or essential genes in a sequence-specific manner via small non-coding RNAs. The method can also be used to enhance beneficial effects and / or growth of symbiotic or commensal bacteria. The invention involves the exogenous delivery of small RNA entities onto bacteria, either in the form of RNA extracts or embedded into plant extracellular vesicles (EVs), so as to reduce bacterial growth, survival and / or pathogenicity. The invention also describes a method to identify in a rapid, reliable and cost-effective manner, small RNAs that possess antibacterial activity and that have the potential to be further developed as anti-infective agents. In addition, the latter method is instrumental to rapidly characterize any gene from any bacterial species.
Owner:CENT NAT DE LA RECHERCHE SCI +2

Symbiotic bacterium composition, preparation method thereof and microalgae culture method

PendingCN113278557AMitigate the risk of pests and diseasesHarm reductionFungiBacteriaBiotechnologyRhodotorula
The invention relates to the technical field of microalgae culture, in particular to a symbiotic bacterium composition, a preparation method thereof and a microalgae culture method. The symbiotic bacterium composition consists of marine clostridium butyricum, marine rhodotorula benthica and marine purple non-sulfur bacteria. Through the symbiotic effect of the three probiotics including the marine clostridium butyricum, the marine rhodotorula benthica and the marine purple non-sulfur bacteria, the risk of diseases and pests in the seawater chlorella culture process is effectively inhibited, the harm of the diseases and pests is reduced, and the capacity and yield of chlorella factory culture are effectively improved.
Owner:HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD

Xinhui citrus health Puer tea and preparation method thereof

PendingCN114376040ARich processing formsRegulate stomachFungiBacteriaBiotechnologyNutritive values
A preparation method of the Xinhui citrus Kangpu tea comprises the following steps: S1, preparing a symbiotic bacterium culture medium: A1, preparing tea liquid; a2, preparing Xinhui orange juice; a3, compounding is conducted; a4, sterilizing; s2, inoculation: B1, primary seed solution culture; b2, culturing a secondary seed solution; b3, strain compounding is carried out; b4, inoculating and fermenting; and S3, performing pasteurization. According to the Xinhui citrus Kangpu tea prepared by the preparation method disclosed by the invention, the resource utilization rate of Xinhui citrus can be fully increased, the unique nutritional values of the green tea, the probiotics and the Xinhui citrus are combined, and on the basis of improving the original bad taste of the Xinhui citrus pulp, the health requirements of modern people on nutritional and healthy beverages are also met. The Xinhui citrus Kangpu tea effectively solves the problem that the Xinhui citrus pulp is sour, astringent and bitter when being directly eaten, is compounded with the green tea and the mulberry leaves, and is fermented into the Xinhui citrus Kangpu tea with double characteristic functions of fermented fruit juice and fermented tea, and the Xinhui citrus Kangpu tea can play roles in regulating intestines and stomach of a human body, removing fat and resisting oxidation.
Owner:广东江门中医药职业学院

Insect pathogenic nematode symbiotic bacterium capable of inhibiting potato black scurf and applications of insect pathogenic nematode symbiotic bacterium

The invention provides an insect pathogenic nematode symbiotic bacterium capable of inhibiting potato black scurf and applications of the insect pathogenic nematode symbiotic bacterium, relating to the insect pathogenic nematode symbiotic bacterium and the applications of the insect pathogenic nematode symbiotic bacterium. The bacterium is Xenorhabdus bovineii HEB01, is preserved in the China General Microbiological Culture Collection Center (CGMCC, located in 3, Courtyard 1, West Beichen Road, Chaoyang District, Beijing,) on Mar 9, 2016, is assigned with the accession number of CGMCC NO.12193, and is used for preventing and treating potato black scurf. The xenorhabdus bovineii HEB01 has the very strong capacity of inhibiting the mycelial growth of the potato black scurf bacterium, the capacity is enhanced along with the increase of the volume concentration, and the antibacterial effect is obvious. Meanwhile, the bacterium has the features that the requirements on the culture conditions are low, the lasting period is long, the source is environmentally friendly and non-toxic, and thus the insect pathogenic nematode symbiotic bacterium has very good development and application prospects.
Owner:黑龙江省农业科学院植物脱毒苗木研究所

Preparation method and product of earthworm breeding companion soil remediation agent

The invention provides a preparation method and a product of an earthworm breeding companion soil remediation agent. The soil remediation agent is used for soil remediation by taking earthworm biomass improvement as a target, a rotten earthworm glandula accessoria ion stress matrix is used as a substrate, an antibacterial earthworm fiber skeleton is filled, an annulata earthworm cocoon synthesis promoting agent benefiting earthworm cocoon, an earthworm symbiotic bacterium agent and an artificial earthworm cocoon barrier are matched, so that the remediation agent is formed. The biomass of the soil earthworm cocoons and earthworms can be increased by 4.40 and 2.9.
Owner:天津天丰泽田生物科技有限公司 +1

Lactobacillus bacterial strain capable of expressing and secreting human interferon Alpha2b and application thereof

Symbiotic bacteria generally mainly producing Lactobacillus bacteria producing H2O2 are distributed on vaginal mucosa of a healthy woman of childbearing age, and the Lactobacillus bacteria functions in protectively preventing urinary and reproductive system infections. Multiple Lactobacillus bacteria are already provided with resistance to, for example, HIV virus by bioengineering so as to prevent the urinary and reproductive system infections. Lactobacillus jensenii producing H2O2, a principle member in the vaginal microbial flora, is reconstructed by bioengineering, so that the Lactobacillus jensenii is enabled to produce and secrete a powerful broad-spectrum antiviral molecule, human IFN Alpha-2b.
Owner:SUZHOU OSEL BIO PHARM

Temperate bacteriophage VneM1 for regulating and controlling coral flora and application of mild bacteriophage VneM1

ActiveCN112011520AImprove high temperature toleranceAntibacterial agentsMicroorganism based processesBiotechnologyHydrozoa
The invention discloses a mild bacteriophage VneM1 for regulating and controlling coral flora and application of the mild bacteriophage VneM1. The preservation number of the bacteriophage is Vibrio neocaledonicus bacteroiphage VneM1 is CGMCC NO.61215-B1. The invention also discloses a preparation method of the bacteriophage. According to the invention, the new bacteriophage-Vibrio neocaledonicus bacteriophage VneM1 is separated from a coral symbiotic bacterium Vibrio neocaledonicus SCSIO 43009. The bacteriophage VneM1 has a cracking effect on coral symbiotic vibrio, and regulates and controlsthe composition of coral symbiotic microorganisms. Hydranth infection experiments prove that the bacteriophage VneM1 can delay coral whitening caused by temperature rise. The bacteriophage VneM1 is amild bacteriophage, and under normal conditions, the bacteriophage VneM1 is integrated on an original lysozyme SCSIO 43009 genome and exists in a form of a prophage. The bacteriophage can be producedby spontaneous lysogen-lysis conversion, and the lysogen-lysis conversion can be promoted by environmental factors such as SOS stress reaction.
Owner:SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI

Algae symbiotic bacterium composition, phycomycete co-culture system and microalgae culture method

PendingCN114164128AFast growthVigorous growth rateFungiBacteriaBiotechnologyRhodotorula
The invention provides an algal symbiotic bacterium composition. The algal symbiotic bacterium composition comprises rhodotorula benthica, a photosynthetic bacterium strain, marine nitrifying bacteria and vibrio. The ratio of the rhodotorula benthica to the photosynthetic bacteria strain to the marine nitrifying bacteria to the vibrio is (20-30) g: (100-200) ml: (10-50) g: (1-3) g; the density of the photosynthetic bacteria strain is 2-5 billion / ml. Compared with the prior art, by means of symbiotic culture of different bacterium combinations and algae, the problems that the microalgae are prone to aging, decaying, breeding of harmful bacteria and the like in the culture process can be solved, the microalgae and beneficial bacteria are subjected to symbiotic culture, then the algae grow faster and more vigorously, and the productivity and yield of the microalgae are improved.
Owner:HAINAN GREEN ALGAE WORLD BIOTECHNOLOGY CO LTD

Biological seed coating agent based on trichoderma-bacillus sequential inoculation symbiotic culture fermentation liquor and preparation method thereof

PendingCN113444649AOvercome antagonismOvercoming is a competitive effectBiocideFungiBiotechnologyTrichoderma sp.
The invention discloses a biological seed coating agent based on trichoderma-bacillus sequential inoculation symbiotic culture fermentation liquor and a preparation method of the biological seed coating agent. According to a synthetic biology principle, a symbiotic bacterium solution is prepared from trichoderma having the characteristics of inducing disease resistance, stress resistance and the like, bacillus having the characteristics of antagonizing pathogenic bacteria, promoting crop growth and the like through a staged inoculation co-fermentation technology; and meanwhile, the symbiotic bacterium solution not only meets the balance of the contents of two types of spores, but also contains components for mutual action and specific induction of two types of microorganisms. The symbiotic bacterium liquid is mixed with diatomite and brassinolide in proportion to prepare the powder type biological seed coating agent, the biological seed coating agent contains high-content spores, antagonistic substances, growth promoting substances and the like, the growth, disease resistance and stress resistance of crops such as corn, wheat, rice and the like are obviously improved, and the technology is a new technology for beneficial bacteria treatment of food crop seeds.
Owner:SHANGHAI JIAO TONG UNIV

Engineered commensal bacteria and methods of use

The present invention provides compositions comprising populations of commensal bacteria isolated from a microbiome sample of a mammalian subject and engineered to express a heterologous polynucleotide, compositions comprising such engineered commensal bacteria and methods of use for delivering a therapeutic polypeptide to a mammal, e.g, by administering the engineered commensal / native bacteria.
Owner:RGT UNIV OF CALIFORNIA

A high-yield cultivation method of pollution-free bletilla striata

The invention discloses a high-yield cultivation method for pollution-free bletilla striata. According to the method, a beneficial symbiotic bacterium nutrient solution obtained after orchidaceae rhizobium or orchidaceae mycorrhizal fungi are subjected to artificial cultivation and separation and humus soil are subjected to propagation fermentation, then nutrient soil is prepared, and after the nutrient soil and soil in planting trenches are mixed, field transplantation of tissue culture seedlings is performed; then intertillage weeding, topdressing and reasonable insect prevention are performed in the growing period, and harvesting can be performed after two years of growth. The method has the advantages that due to combination of symbiotic bacteria and an ecological planting technology, generation of bletilla striata new roots and large tubers are promoted, and meanwhile EM pest preventing liquid is used so that disease and pest damage can be totally avoided; compared with a traditional planting method, the harvested bletilla striata is large in size and small in individual difference, superior products can be over 95%, the yield per mu is increased by 40-50%, and the method has good economic benefits, social benefits and ecological benefits.
Owner:曲靖市马龙区兴裕农业生物科技有限公司

Portable biological crust seed source and preparation method thereof

The invention discloses a portable biological crust seed source and a preparation method of the portable biological crust seed source, and the portable biological crust seed source comprises a biological crust seed source small ball prepared from a stone coat symbiotic bacterium (Endocarpon bacillus) F07020, a stone coat symbiotic alga (Diplosphaera chodatii) A07020 and a forming culture medium. The method is favorable for forming a seed source of the lichens which are collected in a state closer to a natural state, can be applied to desertification land control engineering on a large scale, and is favorable for promoting the formation of artificial biological crust.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI

Method for succulent plant propagation through bacterium and application of bacterium

The invention discloses a method for succulent plant propagation through a bacterium and application of the bacterium. The bacterium is actinomycetes of Homoserinibacter gongjuensis. The propagation method comprises the following steps: (1) explant sterilization and induction: taking explant, inoculating the explant in induction medium after sterilization and inducing to generate embryogenic callus; (2) inoculation and germination: jointly inoculating the Homoserinibacter gongjuensis bacterium and the embryogenic callus in germination medium to form normal cluster bud; (3) strong seedling and transplantation: inoculating the normal cluster buds cultured in the step (2) in strong seedling medium, culturing the cluster buds into strong cluster seedlings which can be transplanted and transplanting. The method disclosed by the invention breaks sterile environment required by general plant tissue culture and effectively solves the problems of general vitrification, slow growth speed and the like in a succulent plant tissue culture process by inoculating the symbiotic bacterium of Homoserinibacter gongjuensis in the succulent plant tissue culture process.
Owner:CHONGQING LANDSCAPE & GARDENING RES INST +1

Tetraodontidae symbiotic bacterium with primary production of tetrodotoxin and application thereof

The invention discloses a tetraodontidae symbiotic bacterium with primary production of tetrodotoxin and application thereof. The tetraodontidae symbiotic bacterium with primary production of tetrodotoxin is named Bacillus cereus He-1, is preserved in the China General Microbiological Culture Collection Center on December 9th, 2019 and has the preservation number of CGMCC NO.19109. The bacillus cereus He-1 is propagated in a culture medium to obtain a bacterial solution of bacillus cereus He-2 which does not produce toxin, and the bacillus cereus is induced to produce tetrodotoxin by adding anautoinducing peptide into the culture solution of bacillus cereus He-2; and the tetrodotoxin producing capacity of the second generation bacterium bacillus cereus He-2 which does not produce toxin isrecovered by adding the autoinducing peptide to serve as a quorum sensing signaling molecule, and tetrodotoxin is produced in a later isolated culture process. The invention solves the problem that atetraodontidae symbiotic bacterium loses the capacity of producing tetrodotoxin after passage of the tetraodontidae symbiotic bacterium with primary production of tetrodotoxin.
Owner:ZHEJIANG WANLI UNIV

Symbiotic bacterium combined fermentation product as well as preparation method and application thereof

The invention provides a symbiotic bacterium combined fermentation product as well as a preparation method and application thereof. The symbiotic bacterium comprises the following components: schizophyllata, Thermus thermophilus, saccharomycetes and lactobacillus. the fermented raw materials comprise rice, jobstears root seeds, ginseng, raspberry and kelp. According to the preparation method disclosed by the invention, a symbiotic bacterium combined fermentation product with relatively high activity is successfully obtained through a three-time combined fermentation process. After the symbiotic bacterium combined fermentation product provided by the invention is applied to a skin care product, the skin care product has the effects of whitening, brightening, resisting oxidation, resisting wrinkles, moisturizing, relieving, diminishing inflammation and the like.
Owner:广州留今科学研究有限公司

A kind of entomopathogenic nematode symbiotic bacteria and application thereof

An entomopathogenic nematode symbiotic bacterium and its application relate to an entomopathogenic nematode symbiotic bacterium and its application. The bacterium is Xenorhabdus budapestensis HEB02, which is preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures. The preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation date is April 11, 2016. , deposit number CGMCC No.12352. For the control of soybean blight. The pathogenic bacterium (Xenorhabdus budapestensis) HEB02 of the present invention has a strong ability to inhibit the growth of soybean damp-off pathogen mycelium, and increases with the increase of the volume concentration, and the antibacterial effect is more obvious.
Owner:FARMING & CULTIVATION RES INST OF HEILONGJIANG ACADEMY OF AGRI SCI

A kind of dsRNA of aphid symbiont gene and its application in inhibiting the growth of aphid

ActiveCN104109671BImprove aphid resistanceBiocideAnimal repellantsBiotechnologyXenorhabdus sp.
The invention discloses dsRNA of a gene of a symbiotic bacterium of aphids and an application thereof to inhibition of growth of aphids. DsRNA provided by the invention is double-stranded RNA formed by a nucleotide shown in a sequence 2 in a sequence table and a nucleotide shown in an inverse complementary sequence of the sequence 2. Experiments prove that the gene of the endotrophic symbiotic bacterium hamiltonella defensa 28469 of wheat aphids is obtained; the gene of the endotrophic symbiotic bacterium hamiltonella defensa 28469 of wheat aphids can be inhibited or silenced, impaired growth and development of the wheat aphids can be caused and lethal effects can be generated by adopting the method of feeding dsRNA in vitro.
Owner:INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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