37 results about "Symbiotic bacterium" patented technology

The invention provides a method for treating sludge digestive liquid by coupling anaerobic ammonia oxidation and anaerobic methane oxidation, and belongs to the technical field of wastewater treatment. The method comprises the steps that anaerobic digested sludge is inoculated into an anaerobic methanogenic reactor, partial nitrification sludge is inoculated into a partial nitrification reactor, and anaerobic ammonia oxidation and denitrification-type methane anaerobic oxidation bacterium symbiotic bacterium group sludge is inoculated into an anaerobic ammonia oxidation coupled anaerobic methane oxidation reactor; firstly the sludge digestive liquid enters the anaerobic methanogenic reactor, after water quality of yielding water is adjusted by a middle water tank, the yielding water enters the partial nitrification reactor, and the nitrosation process is realized by controlling aeration time; finally the water quality of yielding water is adjusted by the middle water tank, the yielding water enters the anaerobic ammonia oxidation coupled anaerobic methane oxidation reactor, meanwhile, methane generated by the anaerobic methanogenic reactor is used for aeration, and the autotrophic nitrogen removal process is completed with the help of anaerobic ammonia oxidation bacteria and anaerobic methane oxidation bacteria. The method can be used for solving the problems of insufficient organic carbon source, relatively low C / N ratio, release of greenhouse gases and the like existing in a sludge digestive liquid nitrogen removal treatment process.

The invention provides a winkle removal composition with a symbiotic bacterium combined ferment substance, a wrinkle removal eye cream and a preparation method of the wrinkle removal eye cream. The wrinkle removal composition comprises the following components in parts by weight: 0.01-5 parts of acetyl hexapeptide-8, 0.1-10 parts of oligopeptide-4, 0.1-10 parts of a crithmum maritimum callus culture filtrate, 0.1-10 parts of dipeptide diamido-butyryl benzyl amide diacetate, 0.01-5 parts of a camellia sinensis leaf extract, 0.1-10 parts of a coffea arabica extract, 0.1-10 parts of a retinol type compound and 1-10 parts of a symbiotic bacterium combined ferment substance. The winkle removal composition is capable of not only promoting c cell proliferation and regeneration and improving skinelasticity, but also effectively preventing oxidation and preventing collagen decomposition. The wrinkle removal eye cream is not only capable of removing dynamic wrinkles, static wrinkles and dry wrinkles which are already formed around eyes, and preventing formation of wrinkles, but also providing long-lasting moisture retention, and making skin tough and healthy.

The invention discloses dsRNA of a gene of a symbiotic bacterium of aphids and an application thereof to inhibition of growth of aphids. DsRNA provided by the invention is double-stranded RNA formed by a nucleotide shown in a sequence 2 in a sequence table and a nucleotide shown in an inverse complementary sequence of the sequence 2. Experiments prove that the gene of the endotrophic symbiotic bacterium hamiltonella defensa 28469 of wheat aphids is obtained; the gene of the endotrophic symbiotic bacterium hamiltonella defensa 28469 of wheat aphids can be inhibited or silenced, impaired growth and development of the wheat aphids can be caused and lethal effects can be generated by adopting the method of feeding dsRNA in vitro.

The invention relates to the technical field of biological prevention and control and especially relates to a use of a fungicide in prevention and control of brown planthopper filial generation. The fungicide which has toyocamycin, tetramycin P, tetrin B and tetramycin A total content of 27% is used for prevention and control of brown planthopper filial generation, wherein toyocamycin content is 5.58% by mass, tetramycin P content is 7.09% by mass, tetrin B content is 4.44% by mass and tetramycin A content is 9.64% by mass. The fungicide comprising toyocamycin, tetramycin P, tetrin B and tetramycin A can prevent or inhibit yeast-like symbiote entrance into ovarian tissue from female brown planthopper adult hemolymph thereby influencing a brown planthopper reproductive system, reducing egg laying amount and substantially reducing egg hatchability because of symbiotic bacterium loss so that brown planthopper filial generation is effectively controlled.

The invention relates to an entomopathogenic nematode symbiotic bacterium XBD102 (Xenorhabdus budapestensis) strain with a broad-spectrum antibacterial function, and the entomopathogenic nematode symbiotic bacterium XBD102 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No: 22057. A fermentation supernatant of the XBD102 strain has a growth inhibition effect on various fungi and bacteria, and can be used for development and application of novel biological pesticide.

A preparation method of the Xinhui citrus Kangpu tea comprises the following steps: S1, preparing a symbiotic bacterium culture medium: A1, preparing tea liquid; a2, preparing Xinhui orange juice; a3, compounding is conducted; a4, sterilizing; s2, inoculation: B1, primary seed solution culture; b2, culturing a secondary seed solution; b3, strain compounding is carried out; b4, inoculating and fermenting; and S3, performing pasteurization. According to the Xinhui citrus Kangpu tea prepared by the preparation method disclosed by the invention, the resource utilization rate of Xinhui citrus can be fully increased, the unique nutritional values of the green tea, the probiotics and the Xinhui citrus are combined, and on the basis of improving the original bad taste of the Xinhui citrus pulp, the health requirements of modern people on nutritional and healthy beverages are also met. The Xinhui citrus Kangpu tea effectively solves the problem that the Xinhui citrus pulp is sour, astringent and bitter when being directly eaten, is compounded with the green tea and the mulberry leaves, and is fermented into the Xinhui citrus Kangpu tea with double characteristic functions of fermented fruit juice and fermented tea, and the Xinhui citrus Kangpu tea can play roles in regulating intestines and stomach of a human body, removing fat and resisting oxidation.

The invention provides a preparation method and a product of an earthworm breeding companion soil remediation agent. The soil remediation agent is used for soil remediation by taking earthworm biomass improvement as a target, a rotten earthworm glandula accessoria ion stress matrix is used as a substrate, an antibacterial earthworm fiber skeleton is filled, an annulata earthworm cocoon synthesis promoting agent benefiting earthworm cocoon, an earthworm symbiotic bacterium agent and an artificial earthworm cocoon barrier are matched, so that the remediation agent is formed. The biomass of the soil earthworm cocoons and earthworms can be increased by 4.40 and 2.9.

The invention provides an algal symbiotic bacterium composition. The algal symbiotic bacterium composition comprises rhodotorula benthica, a photosynthetic bacterium strain, marine nitrifying bacteria and vibrio. The ratio of the rhodotorula benthica to the photosynthetic bacteria strain to the marine nitrifying bacteria to the vibrio is (20-30) g: (100-200) ml: (10-50) g: (1-3) g; the density of the photosynthetic bacteria strain is 2-5 billion / ml. Compared with the prior art, by means of symbiotic culture of different bacterium combinations and algae, the problems that the microalgae are prone to aging, decaying, breeding of harmful bacteria and the like in the culture process can be solved, the microalgae and beneficial bacteria are subjected to symbiotic culture, then the algae grow faster and more vigorously, and the productivity and yield of the microalgae are improved.

The invention discloses a bacterium strain (X-7 system) to culture bait nematolog and nematolog culturing method, which comprises the following steps: culturing Xenorhabdus sp. X-7 CCTCC M 206090 on the culture medium with peptone water under 25-28 deg. c to obtain the bacterial liquid; adding nematolog culture medium; switching in Panagrellus redivivus; culturing under 25-28 deg. c for 6-15h; obtaining the product as bait in the aquatic product culturing industry.

The invention discloses a high-yield cultivation method for pollution-free bletilla striata. According to the method, a beneficial symbiotic bacterium nutrient solution obtained after orchidaceae rhizobium or orchidaceae mycorrhizal fungi are subjected to artificial cultivation and separation and humus soil are subjected to propagation fermentation, then nutrient soil is prepared, and after the nutrient soil and soil in planting trenches are mixed, field transplantation of tissue culture seedlings is performed; then intertillage weeding, topdressing and reasonable insect prevention are performed in the growing period, and harvesting can be performed after two years of growth. The method has the advantages that due to combination of symbiotic bacteria and an ecological planting technology, generation of bletilla striata new roots and large tubers are promoted, and meanwhile EM pest preventing liquid is used so that disease and pest damage can be totally avoided; compared with a traditional planting method, the harvested bletilla striata is large in size and small in individual difference, superior products can be over 95%, the yield per mu is increased by 40-50%, and the method has good economic benefits, social benefits and ecological benefits.

The invention discloses a method for succulent plant propagation through a bacterium and application of the bacterium. The bacterium is actinomycetes of Homoserinibacter gongjuensis. The propagation method comprises the following steps: (1) explant sterilization and induction: taking explant, inoculating the explant in induction medium after sterilization and inducing to generate embryogenic callus; (2) inoculation and germination: jointly inoculating the Homoserinibacter gongjuensis bacterium and the embryogenic callus in germination medium to form normal cluster bud; (3) strong seedling and transplantation: inoculating the normal cluster buds cultured in the step (2) in strong seedling medium, culturing the cluster buds into strong cluster seedlings which can be transplanted and transplanting. The method disclosed by the invention breaks sterile environment required by general plant tissue culture and effectively solves the problems of general vitrification, slow growth speed and the like in a succulent plant tissue culture process by inoculating the symbiotic bacterium of Homoserinibacter gongjuensis in the succulent plant tissue culture process.

An entomopathogenic nematode symbiotic bacterium and its application relate to an entomopathogenic nematode symbiotic bacterium and its application. The bacterium is Xenorhabdus budapestensis HEB02, which is preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures. The preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation date is April 11, 2016. , deposit number CGMCC No.12352. For the control of soybean blight. The pathogenic bacterium (Xenorhabdus budapestensis) HEB02 of the present invention has a strong ability to inhibit the growth of soybean damp-off pathogen mycelium, and increases with the increase of the volume concentration, and the antibacterial effect is more obvious.

The invention discloses dsRNA of a gene of a symbiotic bacterium of aphids and an application thereof to inhibition of growth of aphids. DsRNA provided by the invention is double-stranded RNA formed by a nucleotide shown in a sequence 2 in a sequence table and a nucleotide shown in an inverse complementary sequence of the sequence 2. Experiments prove that the gene of the endotrophic symbiotic bacterium hamiltonella defensa 28469 of wheat aphids is obtained; the gene of the endotrophic symbiotic bacterium hamiltonella defensa 28469 of wheat aphids can be inhibited or silenced, impaired growth and development of the wheat aphids can be caused and lethal effects can be generated by adopting the method of feeding dsRNA in vitro.

The invention discloses a method for succulent plant propagation through a bacterium and application of the bacterium. The bacterium is actinomycetes of Homoserinibacter gongjuensis. The propagation method comprises the following steps: (1) explant sterilization and induction: taking explant, inoculating the explant in induction medium after sterilization and inducing to generate embryogenic callus; (2) inoculation and germination: jointly inoculating the Homoserinibacter gongjuensis bacterium and the embryogenic callus in germination medium to form normal cluster bud; (3) strong seedling and transplantation: inoculating the normal cluster buds cultured in the step (2) in strong seedling medium, culturing the cluster buds into strong cluster seedlings which can be transplanted and transplanting. The method disclosed by the invention breaks sterile environment required by general plant tissue culture and effectively solves the problems of general vitrification, slow growth speed and the like in a succulent plant tissue culture process by inoculating the symbiotic bacterium of Homoserinibacter gongjuensis in the succulent plant tissue culture process.