70 results about "Scenedesmus obliquus" patented technology

The invention discloses a water comprehensive toxicity rapid detection method based on algae chlorophyll fluorescene. According to the method, toxic and sensitive unicellular scenedesmus obliquus is selected as a testing organism, an experimental group and a blank control group of a to-be-tested water sample are subjected to adaptive cultivation for 24 hours, fast-phase chlorophyll fluorescene of two groups of algae samples is measured, fluorescence parameters in the energy transfer process of main photosynthesis are analyzed and obtained, an inhibition ratio of to-be-tested water of the experimental group on the photosynthesis of algae is calculated, and the comprehensive toxicity intensity of the to-be-tested water is calculated according to the dose-effect relationship between the substance toxicity intensity and the inhibition ratio of photosynthesis. The method has the characteristics of sensitive toxicity response, simple and fast test and the like and is a new method for rapid evaluation of ecological risk and emergency monitoring of sudden pollution accidents.

The invention relates to a method for preparing biochemical preparations for treating threonine fermentation wastewater. The method includes preparing physical adsorbents and complex microbial inoculants. The complex microbial inoculants comprise, by volume, 6-7 parts of yeast, 5-6 parts of arthrobacter, 5-6 parts of pseudomonas, 4-5 parts of rhodococcus, 4-5 parts of bacillus amyloliquefaciens, 4-5 parts of clostridium and 3-4 parts of scenedesmus obliquus. The method has the advantages that the biochemical preparations contain diversified microorganisms with excellent degradation capacity for refractory pollutants, various strains are reasonably compatible with one another, accordingly, excellent degradation effects can be realized, and the method has a broad application prospect.

The invention relates to a method for producing microalgae grease by using flue gas. The method for the producing microalgae grease by using the flue gas comprises the following steps that firstly, amicroalgae culture medium and parachlorella kessleri FSH-Y3 or / and a scenedesmus obliquus FSH-Y2 seed solution are added into a photobioreactor, the pH value is adjusted to 10-12, the flue gas with CO2 content of 1v-5v% is introduced, and culture is carried out for a certain time; and then the pH value is adjusted to 8-10, a chlorella SF-B1 seed solution is introduced, at least one of a scenedesmus MH-04 seed solution a monoraphidium SS-B1 seed solution is introduced, mixed culture is carried out, the flue gas with CO2 content of 5v%-45v% is introduced, and the mixture is cultured to a stableperiod under the condition of continuous illumination, so that microalgae cells are obtained. The method for producing the microalgae grease by using the flue gas improves the tolerance and solubilityof a microalgae culture system to high-concentration CO2, improves the carbon fixation efficiency, obviously improves the harvest amount of the microalgae grease, simultaneously improves the tolerance to SOx and NOx in the flue gas, and can purify the flue gas.

The invention discloses a treatment method of aquacultural waste water. According to the treatment method, three kinds of algae, including chlorella pyrenoidosa, scenedesmus obliquus, and pseudokirchneriella subcapitata, are used for treating aquacultural waste water. According to the treatment method, a mixture of the three kings of algae is capable of removing substances in different forms, such as nitrogen and phosphor, in waste water; the treatment method is simple and effective, is low in cost, and is high in practicality; and the algae can be taken as food of aquatic animals.

The invention provides a novel ecologic garden greening soil remediation treatment agent composed of, by mass, 8-12 parts of glycerol, 20-22 parts of metalaxyl-M, 16-20 parts of fenarimol, 14-18 partsof antibacterial peptide, 14-17 parts of zeolite, 8-12 parts of polyethylene oxide, 20-24 parts of ammonium phosphate, 18-22 parts of potassium silicon fertilizer, 18-22 parts of artemisia vulgaris essential oil, 16-20 parts of beer distiller grain, 18-22 parts of scenedesmus obliquus, 18-22 parts of coconut coir, 16-20 parts of chili extract, 18-22 parts of chestnut shells, 18-22 parts of castoroil, 16-20 parts of diethylaminoethanol caproate, 22-24 parts of diatomite, 16-20 parts of aluminum sulfate, 16-20 parts of volcanic ash, 18-22 parts of ammonium dihydrogen phosphate and the like. Inpractical use, the treatment agent can complex and immobilize metal elements in soil onto bamboo fibers, so that heavy metal concentration of soil is rapidly reduced and microorganisms in soil are protected. The treatment agent is suitable for large-scale promotion and application.

The invention relates to a method for biologically eliminating and controlling the eutrophication of an open water body on the basis of a daphnia magna food chain relationship according to the principle of in-situ ecosystem remediation, and particularly relates to a daphnia magna domestication method. The method comprises the following steps: selecting a target water body to be treated; monitoring the water quality and pollution sources of the water body; according to the water quality and the pollution sources, domesticating and cultivating daphnia magna by stages with scenedesmus obliquus and eutrophic water mixed liquids until the daphnia magna is completely adapted to the environmental condition of the target water body to be treated, wherein the scenedesmus obliquus and eutrophic water mixed liquids have different scenedesmus obliquus concentrations; then continuously, regularly and uniformly putting the daphnia magna into the target water body according to a low concentration; and finally introducing specific fish, such as aristichthysnobilis, which feeds on the daphnia magna. Through the method, the problem of eutrophication of the open water body can be solved effectively, an original ecosystem is recovered, and in-situ remediation of the water body is realized.

The invention discloses a plant growth regulator for promoting the growth of water plants and beneficial algae in shrimp and crab ponds and relates to the field of aquaculture. The water plants comprise waterweed. The beneficial algae comprise chlorella pyrenoidosa and scenedesmus obliquus. The plant growth regulator is a mixture comprising any one or more of diethyl aminoethyl hexanoate, compound nitrophenol, gibberellin and brassinolide. The use amount of the plant growth regulator used to promote the growth of the beneficial algae is in a range of 0.01 to 1 mg / L, and the use amount of the plant growth regulator for promoting the growth of the waterweed is in a range of 0.001 to 0.06 mg / L. The plant growth regulator can effectively promote the increasing of fresh weight of the waterweed, plant height and the growth of fibril, can observably promote the growth of chlorella pyrenoidosa and scenedesmus obliquus, can increase the density of chlorella pyrenoidosa and scenedesmus obliquus in valid time, and can improve the survival rate and growth rate of juvenile fish, shrimps and crabs.

The invention discloses a formula for treating waste water for aquaculture. According to the treatment method, chlorella pyrenoidosa, scenedesmus obliquus and selenastrum capricornutum are adopted for interaction treatment and culture, and experimental research is done on the waste water. The formula for treating waste water for aquaculture is capable of effectively eliminating substances such as nitrogen and phosphorus existing in the waste water in different forms, and has the advantages of being simple, effective, low in cost, high in practicality and the like; moreover, algae grows and can serve as food of aquatic animals, cost is low, the effect is remarkable, and the living environment of the animals and plants is not influenced.

The invention provides application of artemether as an algae inhibiting agent. Artemisinin simple ether derivatives have a good inhibition effect on copper aluminum microcystis, scenedesmus obliqnus and the like. The artemisinin simple ether derivatives can be prepared into missible oil, clathrate, slow release microcapsules and the like as algae inhibiting agents applied in nature water bodies like ponds, reservoirs and lakes for inhibiting algae booms.

The enzyme, iron hydrogenase (HydA), has industrial applications for the production of hydrogen, specifically, for catalyzing the reversible reduction of protons to molecular hydrogen. The present invention relates to the isolation of a nucleic acid sequence from the algae Scenedesmus obliquus, Chlamydomonas reinhardtii and Chlorella fusca that encodes iron hydrogenase. The invention further discloses the genomic nucleic acid, c-DNA and the protein sequences for HydA. The genes and gene products may be used in a photosynthetic process for hydrogen production which includes growing a microorganism containing the gene coding for HydA in a culture medium under illuminated conditions sufficient to accumulate an endogenous substrate; depleting a nutrient selected from the group consisting of sulfur, iron, and manganese from the medium; then allowing the culture to become anaerobic by consumption of an endogenous or exogenous substrate in the light.

The invention relates to an open-type method for producing microalgae oil. The method comprises the following steps: (1) a microalgae culture medium and parachlorella kessleri FSH-Y3 or / and a scenedesmus obliquus FSH-Y2 seed solution are added to an open reaction tank, the pH is adjusted to 10 to 12, the temperature is 20 to 30 DEG C, and gas having a volume of CO 2 being 1 v% to 5 v% is introduced for culturing for a certain period of time; and (2) the pH of a culture system is adjusted to 8 to 10, the temperature is 0 to 20 DEG C, and a chlorella SF-B1 seed solution and a single needle algaeSHJ-02 seed solution are inoculated, the gas with a volume of CO2 of 5v% to 45v% is introduced, and culturing is carried out under light conditions to obtain the microalgae cells. The method of the invention improves the tolerance and solubility of the microalgae culture system to high concentration of CO2, improves the carbon fixation efficiency and the oil yield, and can inhibit the contamination of the bacteria during the cultivation process.

The invention provides a method for producing natural carotenoids by culturing of Scenedesmus obliquus New which is newly domesticated scenedesmus obliquus. The method includes that yellow serofluid pretreatment, pH adjustment, adding of rich-selenium culture (obtained from 'the world selenium city' Enshi), sterilization and lateral lighting culture with red-light lamp tubes on two sides are performed; carotenoid induction is carried out through methyl jasmonate, top-lighting blue light 7600lux in light-dark period of 21h:3h, temperature stimulation at 32-37 DEG C, nitrogen starvation, glucoseand xylose; algae cell low-temperature high-pressure homogenization wall breaking or ultrasonic-assisted extraction of carotenoids is performed to prepare a dried product of rich-selenium carotenoids. In consumption of per gram of scenedesmus obliquus in dry weight, 123.12mg of the dried product of Scenecarotenoid which is novel rich-selenium natural carotenoids can be obtained. The method is remarkably helpful to production of novel products of rich-selenium natural carotenoids, and a promising development prospect and an extremely high market value are achieved.

The invention relates to the technical field of wastewater detection, and in particular relates to a garbage leachate toxicity evaluation method. The garbage leachate toxicity evaluation method comprises the following steps of (1) preparing test sample liquid from garbage leachate; (2) performing quantitative in-vitro biotoxicity evaluation on the test sample liquid through a recombined gene spore; (3) exposing early developed fish serving as a tested biological material in the test sample liquid, checking growing development reproduction conditions and genetic transcription change conditions of the fish, so as to obtain a real quantitative detection result of garbage leachate toxicity; and (4) exposing scenedesmus obliquus-moina macrocopa straus-zebra fish serving as a tested simulated ecological system in the test sample liquid, and evaluating a biological enrichment and generative transmission effect of garbage leachate toxicity substances, so as to obtain a relevant detection result. The method disclosed by the invention has multiple advantages of quickness, high efficiency, precision in quantification, truth, reliability, multi-layer evaluation and the like, and has the extremely large practical value and the wide market prospect.

The invention provides a method for fluorescence detection of biotoxicity of desmetryn by scenedesmus obliquus in ammonium salt environment, and belongs to the technical field of scenedesmus obliquus. The method comprises the steps of scenedesmus obliquus seed culture and scenedesmus obliquus liquid preparation, and also comprises influence determination of the desmetryn on the scenedesmus obliquus fluorescence effect in the ammonium salt environment. According to the method, the series concentration of desmetryn standard solutions added with ammonium salt solution are taken and are added into the scenedesmus obliquus solution; sufficient mixing and uniform shaking are performed; the volume ratio of the ammonium salt solution to the desmetryn standard solution to the scenedesmus obliquus liquid is controlled to be 2:(1 to 2):(1 to 2); meanwhile, distilled water is used for replacing a blank control group of the scenedesmus obliquus standard solution; after the dark placement for 10 to 15min, a water sample fluorescence detection instrument is used for determining each chlorophyll fluorescence dynamic parameter of the scenedesmus obliquus. The method provided by the invention has the advantages that the method is simple; the operation is easy; the biotoxicity of the desmetryn can be directly reflected.

The invention discloses a method for evaluating toxicity of single triazole bactericide agricultural chemicals by using mixed green alga. Three kinds of algae (mixed green alga) of Selenastrumcapricornutum (Selenastrumcapricornutum), Scenedesmus obliquus ( Scenedesmus obliquus ) and Chlorellapyrenoidosa (Chlorellapyrenoidosa) are subjected to mixed culture, to evaluate the single toxicity of three typical agricultural chemical triazole bactericides (triazolone, myclobutanil, tebuconazole ). Under the action of the triazole bactericide agricultural chemicals, for the half effective concentration EC50 of the mixed green alga, the single toxicity of the triazole bactericide agricultural chemicals is evaluated. The method considers the monopoly of single algae kinds in a natural water body, and varied algae are in intergrowth, so that a mixed culture method closed to the environment of the natural water body is adopted to objectively and comprehensively evaluate the potential threaten of pollutants. The method also has the advantages of being simple and quick to test, saving consumption of expensive chemicals, and the like toxotest is convenient to repeatedly and parallelly performed, so that the test results are accurate.

The invention discloses antibacterial and algae-killing active carbon and a preparation method thereof, which comprises the following steps: applying acrylonitrile-styrol reisn to the surface of active carbon; performing chemical treatment so as to combine the acrylonitrile-styrol reisn with copper ions. The acrylonitrile-styrol reisn is firmly combined on active carbon, thus being safe and nontoxic to the environment. The antibacterial and algae-killing active carbon has an antibacterial effect on tested golden staphylococcus, colon bacillus, white candida, micrococcus lutea, bacillus subtilis, etc., and has a good effect of killing various types of algae such as microcystic aeruginosa DS, microcystic aeruginosa 7820, Plectonemaboryanum IU594, chlammydomonas reinhardtii, scenedesmus obliquus, etc., which cause algae bloom in fresh water. The antibacterial and algae-killing active carbon can be used for sterilization and treatment of algae bloom in fresh water in environmental cleaning.

The invention relates to the biological treatment process of xanthan gum fermentation waste. According to the biological treatment process, feeds are prepared from zymophyte proteins, and sewage is drained after being subjected to biological treatment and physical treatment. A biological treatment agent is prepared from the following raw materials in volume ratio: 5 parts of pseudomonas aeruginosa, 3 parts of penicillium ochrochloron, 3 parts of acidithiobacillus ferrooxidans, 3 parts of rhodococcus, 4 parts of bacillus amyloliquefaciens, 2 parts of clostridium glycolicum and 1 part of scenedesmus obliquus. The biological treatment agent contains microorganisms with excellent degradation capacity to non-biodegradable pollutants; and by virtue of reasonable compatibility of strains, the biological treatment agent has good degradation effect and wide application prospect.

The invention discloses a liquid conditioning agent improving a soil structure of a secondary salinization land. The liquid conditioning agent is prepared from the following raw materials in parts by mass: 120 to 180 parts of crop waste pulp, 250 to 400 parts of decomposed excrement, 160 to 200 parts of nano carbon, 120 to 160 parts of scenedesmus obliquus decomposition agent, 80 to 120 parts of tealeaf residues, 30 to 60 parts of EDTA-Zn, 8 to 20 parts of azotobacter chroococcum, 800 to 1200 parts of water and 10 to 14 parts of microorganism fermenting agent. The liquid conditioning agent can reduce the salt content of soil, the pH value of the soil and the volume weight of the soil, can effectively increase permeable holes of the soil, can improve the water retaining capacity of the soil, can increase the content of microorganisms in the soil, and can greatly and comprehensively improve the fertility of the soil; and after the liquid conditioning agent is used, the soil fertility is improved, the immunity of crops of agriculture and forestry can be improved, the grain yield is increased, and the energy-saving, environment-friendly and sustainable development trend can be accorded with.

The invention relates to a strain of Scenedesmus obliquus producing a self-flocculation substance, and an application of the Scenedesmus obliquus in microalgae harvesting, and belongs to the technical field of microbial applications. The strain has a classification name of Scenedesmus obliquus and a preservation number of CGMCC No.6567, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on September 14, 2012, wherein the address is Institute of Microbiology, Chinese Academy of Sciences, 3# Court No.1, Beichen West Road, Chaoyang District, Beijing. With a flocculation substance produced by the Scenedesmus obliquus AS-6-1, non-flocculation strains such as freshwater scenedesmus FSP-3, freshwater chlorella CNW-11 and seawater chlorella DUT01 can be subjected to rapid flocculation harvesting so as to provide broad application prospects in microalgae harvesting. The flocculation substance is separated and purified from the self-flocculation Scenedesmus obliquus AS-6-1 cell wall, and is a water-soluble polysaccharide, wherein the non-flocculation scenedesmus FSP-3 is treated with 0.6 mg / L of the flocculation substance, and the flocculation efficiency can reach 80%; and the most suitable pH value is 7.0, the most suitable temperature is 30 DEG C, the flocculating activity does not require metal ions, and characteristics of time saving, efficiency, good pH value stability and good thermal stability are provided.

The invention relates to a separation purifying method of a scenedesmus obliquus strain SoHNCJ2. The separation purifying method includes: diluting a sampled water sample, evenly coating a BG11 culture medium flat plate of 1.5-2% agar with the diluted water sample, and placing into a cultivation box for continuous illumination cultivation; using a toothpick to pick a single colony after the single colony grows on the flat plate, and cultivating the single colony on the BG11 flat plate by drawing a short streak; using an inoculation needle to perform separation and sterilizing on cultivated algae species by drawing a Z-shaped streak, and using a dilution and flat plate coating method to further purify the algae species; picking the single colony to draw a short streak, picking the short-streak colony to draw a Z-shaped streak, and alternating the process for 2-4 times to obtain the purified single colony. A cultivation method of the scenedesmus obliquus strain SoHNCJ2 is characterized in that the concentration of N or P of the fresh water cyanobacteria culture medium BG11 is lowered to 1 / 4 of the original concentration or increased to 3 times of the original concentration. The cultivated scenedesmus obliquus strain can grow continuously and fast under a low-concentration N source condition, algae cells can effectively accumulate a large amount of grease, and grease conversion production can be performed to produce biodiesel.

The invention relates to an antibacterial and insecticidal slow release ecologic agriculture and forestry fertilizer. The antibacterial and insecticidal slow release ecologic agriculture and forestry fertilizer comprises methyl jasmonate, a potassium magnesium sulfate fertilizer, lutein ester, biochemical potassium fulvate, argy wormwood essential oil, brown seaweed polysaccharides, triptolide, wheat stalks, Scenedesmus obliquus, carbon residues, mint, zinc glycinate, dimethyl fumarate, thiamine hydrochloride, chitosan, potassium fulvate, sodium carbonate powder, trehalose, disodium octoborate tetrahydrate, lignocellulose, feldspar powder, ammonium ferrous sulfate, potassium sulfate, borax, thiamethoxam, indoxacarb, tolclofos-methyl, itaconic acid, propamocarb hydrochloride, cellulose acetate, yellow humic acid, n-octyl alcohol, diaminodiphenylmethane and sodium potassium tartrate. A preparation method of the above product is simple, and the produced product has superior soil repairing ability and good insecticidal performance, supplies nutritional elements needed by agriculture and forestry crops, greatly increases the survival rate of the agriculture and forestry crops, and improves the use effect.

The invention provides a straw biological decomposing method adopting a scenedesmus obliquus decomposing agent. The method comprises: paving straws; and adding the scenedesmus obliquus decomposing agent which comprises the raw materials in parts by mass: 28-36 parts of scenedesmus obliquus, 6-9 parts of a composite fungicide, 30-38 parts of rhizoma phragmitis and 25-31 parts of camellia pollen. According to the straws decomposed by virtue of the straw biological decomposing method, the content of organic carbon is 295-315 k / kg, the mineralization rate is 17.1-22.4%, the content of total nitrogen is 18.28-19.96 g / kg, C / N is 14.78-17.23, and T value is 0.45-0.52; the germination rate of seeds is 94.1-95.7%. According to application of the straw biological decomposing method in conditioning a soil structure of a saline and alkaline land, the salt content, the pH and the volume weight of the soil can be reduced, the gas permeability and the microbial content of the soil are increased, and the water retaining capacity and the fertility of the soil are improved.

The invention belongs to the technical field of biology, and discloses a process for producing hydrogen by cooperatively using scenedesmus obliquus and fungus. The process comprises the following steps: (1) preparing compound fungus liquid; (2) preparing algae liquid; (3) preparing hydrogen production culture solution; (4) producing the hydrogen by cooperatively using the materials. The process provided by the invention is simple and feasible, and high in hydrogen production efficiency, thus having wide application scope.

The invention discloses a method for testing the biological toxicity of sulfonamide antibiotics by using scenedesmus obliquus. With the sulfonamide antibiotics as a research object, the scenedesmus obliquus as a test organism and a 96-hole microplate as a carrier, through a 96-hole microplate sampling analysis method, an absorbance value of the scenedesmus obliquus at 681nm is tested by using a multi-function microplate reader after sampling for 96h, the growth inhibition ratio of the scenedesmus obliquus is calculated by using absorbance values of an experimental group and a control group, anegative logarithm pEC50 of an EC50 value is fitted and calculated by using a Matlab-based self-programmed Logit function, and the magnitude of the toxicity of the sulfonamide antibiotics is judged with the pEC50 value as a toxicity indicator. The method has the advantages of simple operation, convenience and quickness and good repeatability and can be widely applied to test the toxicity of the sulfonamide antibiotics.

The invention relates to a salting method of a 2-(1-amido-ethyl)-N-phenyl thiazole-4-methanamide compound and a derivative thereof, and application thereof in the aspects of prevention and treatment of limnetic algae. The 2-(1-amido-ethyl)-N-phenyl thiazole-4-methanamide compound is a compound shown in a structural general formula, wherein X is HCl, H2SO4, H3PO4, maleic acid, fumaric acid, para-toluenesulfonic acid, citric acid, oxalic acid, malic acid, acetic acid, tartaric acid and the like; R, R1, R2 and R3 are H, halogens, C2-C5 alkyls, sulfo groups, alkoxy, nitryl, amino, substituted amino and the like. The salifying method of the compound is simple and convenient in process, and applicable to industrial production, and has the potential of developing an efficient algicide. The 2-(1-amido-ethyl)-N-phenyl thiazole-4-methanamide compound has excellent broad-spectrum killing algae activity on limnetic algae chlorophyta verdigris microcystin algae, scenedesmus obliquus, chlorella pyrenoidosa and the like. Compared with the traditional algicide, the 2-(1-amido-ethyl)-N-phenyl thiazole-4-methanamide compound has the characteristics of being high in activity, friendly to environment, low in toxicity on non-target organisms and the like, simple in synthetic method, and is an algicide lead compound with value.

The invention belongs to the technical field of biological environment protection and discloses a biological agent for treating ash sluicing water of a cable channel. The biological agent is prepared by culturing thiobacillus ferrooxidans, thiobacillus thiooxidans, pseudomonas aeruginosa, sphingomonas and scenedesmus obliquus and mixing with a specific carrier. The biological agent can be used for effectively treating ash sluicing waste water.

The invention provides a method for determining a toxic effect of scenedesmus obliquus by copper ions in different fresh water environments, and an application. The morphology and concentration of copper, competitive positive ions and the influence of pH on the toxicity of the scenedesmus obliquus are comprehensively considered; and an obtained toxicity prediction model is verified in an experiment and has high accuracy in weak alkaline fresh water with the pH value higher than 7.