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Process for producing hydrogen by cooperatively using scenedesmus obliquus and fungus

A technology of Scenedesmus obliquus and process, applied in the biological field, can solve the problems of low hydrogen production efficiency of algae, and achieve the effects of good synergy performance, promotion of algal biomass, and good hydrogen production capacity

Active Publication Date: 2018-04-20
济南新金氢能源有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the defects of low hydrogen production efficiency of algae in the prior art, and provide a process for synergistic hydrogen production using Scenedesmus obliques and fungi

Method used

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  • Process for producing hydrogen by cooperatively using scenedesmus obliquus and fungus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The technique for synergistically producing hydrogen by using Scenedesmus obliquus and fungi comprises the following steps:

[0035] Preparation of composite bacterial solution: Enterococcus faecium and Rhodopseudomonas palustris were cultured separately to obtain 1×10 8 The seed liquid of CFU / ml is then mixed according to the volume ratio of 1:2 to obtain the mixed seed liquid, and then transferred to the fermentation medium according to the inoculation amount of 10%, and cultivated at 30°C for 12h to obtain the composite bacterial liquid; the fermentation medium The formula by mass percentage is: molasses 3%, corn steep liquor 2.5%, soybean meal 2%, K 2 HPO 4 0.1%, KH 2 PO 4 0.1%, CaCO 3 0.01%, FeSO 4 0.005%, MnSO 4 0.005%, the balance is water, pH7.0;

[0036] Preparation of algae liquid: Take Scenedesmus obliquus in the logarithmic growth phase, inoculate it into the TAP culture medium according to the inoculum amount of 5% for cultivation, and control th...

Embodiment 2

[0040] The technique for synergistically producing hydrogen by using Scenedesmus obliquus and fungi comprises the following steps:

[0041] Preparation of composite bacterial solution: Enterococcus faecium and Rhodopseudomonas palustris were cultured separately to obtain 1×10 8 The seed liquid of CFU / ml is then mixed according to the volume ratio of 1:2 to obtain the mixed seed liquid, and then transferred to the fermentation medium according to the inoculation amount of 10%, and cultivated at 30°C for 12h to obtain the composite bacterial liquid; the fermentation medium The formula by mass percentage is: molasses 3%, corn steep liquor 2.5%, soybean meal 2%, K 2 HPO 4 0.1%, KH 2 PO 4 0.1%, CaCO 3 0.01%, FeSO 4 0.005%, MnSO 4 0.005%, the balance is water, pH7.0;

[0042] Preparation of algae liquid: Take Scenedesmus obliquus in the logarithmic growth phase, inoculate it into the TAP culture medium according to the inoculum amount of 7% for cultivation, and control th...

Embodiment 3

[0055] Hydrogen production performance test:

[0056] The liquid volume of setting reactor is 8L, and the space above liquid is 2L, and the amount of gas produced in the reaction process is obtained by multiplying the gas volume by the gas composition, and the gas phase product inside the reactor is measured by gas chromatography;

[0057] The hydrogen production and hydrogen content of Example 1 and Comparative Examples 1-2 were respectively detected; the concentration of algae cells in the culture solution was also detected by cell counting. See Table 1 for details:

[0058] Table 1

[0059] group

[0060] Conclusion: the amount of hydrogen produced in Example 1 of the present invention is significantly better than that of Comparative Example 1 and Comparative Example 2, and the concentration of algae cells is also better than that of Comparative Example 1 and Comparative Example 2. The activity of hydrogen-producing enzymes can increase the hydrogen production. ...

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Abstract

The invention belongs to the technical field of biology, and discloses a process for producing hydrogen by cooperatively using scenedesmus obliquus and fungus. The process comprises the following steps: (1) preparing compound fungus liquid; (2) preparing algae liquid; (3) preparing hydrogen production culture solution; (4) producing the hydrogen by cooperatively using the materials. The process provided by the invention is simple and feasible, and high in hydrogen production efficiency, thus having wide application scope.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a process for synergistically producing hydrogen by using Scenedesmus obliques and fungi. Background technique [0002] With the development of industrialization and the improvement of human living standards, the use of fossil energy is also increasing year by year. On the one hand, it has caused serious environmental pollution, and on the other hand, fossil energy is non-renewable. Therefore, it is very urgent to find a clean and efficient renewable new energy. Compared with other energy substances, hydrogen has a series of advantages such as rich resources, low density, light weight, easy transportation, various utilization forms, wide range of uses, high combustion value, clean and renewable, and only produces water after combustion, so it is considered to be The most potential environmental protection renewable energy in the future society. With the further developm...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P3/00C12R1/01C12R1/46C12R1/89
CPCC12P3/00C12P39/00
Inventor 黄永根洪迪明胡三强白雪高白健明白永炜
Owner 济南新金氢能源有限责任公司
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