93 results about "Enterococcus" patented technology

Compositions comprising 5. salivarius strain SALI-10 and / or salivarius 10 peptide are taught. Also taught are methods of treating bacterial infections comprising administering a pharmaceutically acceptable amount of the composition to the patient with a bacterial infection caused by a pathogen from, for example, multi-drug resistant (MDR) Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus dysagalactiae, vancomycin-resistant Enterococcus faecium, Porphyromonas gingivalis, Tannerella forsythia, and MDR Enterococcus faecalis.

The invention relates to the technical field of microorganisms, in particular to enterococcus faecalis capable of degrading uric acid and application of the enterococcus faecalis, the enterococcus faecalis is named as Enterococcus faecalis SX3, and the preservation number of the enterococcus faecalis is CCTCC NO: M 2025248. The enterococcus faecalis has the advantages of being wide in antibacterial spectrum, rapid in growth, high in stress resistance, high in biological safety and the like, and important application value is provided for formulating a scheme for degrading uric acid.

The invention discloses a composition for treating hyperuricemia nephropathy and application thereof, and belongs to the field of biological medicines.The composition is prepared from, by weight, 28-32 parts of rhizoma smilacis glabrae, 28-32 parts of yam rhizome, 13-17 parts of radix clematidis, 8-12 parts of peach kernels, 8-12 parts of flos carthami, 8-12 parts of herba lycopi, 18-22 parts of rhizoma alismatis, 28-32 parts of raw coix seeds, 28-32 parts of semen plantaginis, 13-17 parts of rhizoma atractylodis and 13-17 parts of pseudobulbus cremastrae seu pleiones. The composition can obviously reduce the uric acid level of a mouse with high uric acid nephropathy and improve kidney injury; meanwhile, intestinal flora of mice with the hyperuricemia nephropathy can be remarkably remodeled, abundance of beneficial bacteria Ackerman's bacteria is up-regulated, abundance of opportunistic pathogenic bacteria such as escherichia coli-shigella, desulfurization vibrio and enterococcus is down-regulated, and important significance is achieved for research and drug development of the hyperuricemia nephropathy.

The invention relates to a composite microbial inoculant and an evaluation method for the antibacterial effect thereof. The method optimizes the in-vitro culture mode of the microbial agent compounded by aerobic bacteria and anaerobic bacteria by simulating the intestinal environment of probiotics in an animal body, and adopts a facultative anaerobic culture method for the microbial compound agent, namely, the outer layer of a sealing film of a triangular flask is wrapped with four layers of preservative films, and the neck of the triangular flask is tightened by a rubber band. The facultative anaerobic environment of aerobic bacillus and facultative anaerobic enterococcus faecalis is considered in the microbial composite inoculant, in the subsequent oxford cup bacteriostasis evaluation experiment, the experiment result with the accurate bacteriostasis effect and good parallelism can be obtained, and the action effect of bacillus and enterococcus faecalis composite bacteria can be efficiently and scientifically evaluated.

The microorganism strain Pediococcus acidilactici TAK 589 CoccobEst is a new antimicrobial and antioxidant probiotic, CoccobEst inhibits the growth of the enteropathogens L. monocytogenes, E. coli, S. enteritidis, S. typhimurium, S. sonnei, S. aureus, E. faecalis and C. sakazakii, but does not inhibit lactobacilli of natural intestinal microbiota. The strain's antimicrobial properties are enhanced by periplasmic peptidoglycan hydrolases with proteolytic activity. CoccobEst is used as a zootechnieai feed additive to prevent bacterial infections in the gastrointestinal tract of farm animals and pets and to prevent and reduce diarrhea. CoccobEst has highly active antioxidant defense system, increasing the redox activity of glutathione and reducing oxidative stress in cells. Therefore, CoccobEst is used as a food supplement or functional food for humans to prevent bacterial infections of the gastrointestinal tract and to prevent and reduce oxidative stress. In addition, CoccobEst is used as a starter culture in fermentation of vegetables.

An object of the present invention is to provide a new lytic agent capable of lysing Enterococcus bacteria. Provided is a lytic agent for Enterococcus bacteria, consisting of a lytic enzyme or an active fragment thereof, the lytic enzyme comprising: (a) the amino acid sequence shown in SEQ ID NO: 1, (b) an amino acid sequence in which one or multiple amino acids are added, deleted, and / or substituted in the amino acid sequence shown in SEQ ID NO: 1, or (c) an amino acid sequence having a sequence identity of 90% or more with the amino acid sequence shown in SEQ ID NO: 1.

The invention relates to the technical field of microorganisms, in particular to mixed bacteria fermentation liquor as well as a preparation method and application thereof. Bacterial strains in the mixed fermentation liquor comprise clostridium butyricum, enterococcus faecium and bacillus. The preparation method of the mixed bacteria fermentation liquor comprises the following steps: culturing the strains in the mixed bacteria fermentation liquor in a mixed bacteria fermentation culture medium to obtain the mixed bacteria fermentation liquor; wherein the bacterial strains comprise clostridium butyricum, enterococcus faecium and bacillus. According to the mixed bacterium fermentation liquor and the preparation method and application thereof, coexisting culture of donkey source enterococcus faecium, donkey source bacillus and donkey source clostridium butyricum can be achieved through the mixed bacterium fermentation liquor, and meanwhile the mixed bacterium fermentation liquor has the excellent effects of producing compound enzyme and inhibiting pathogenic bacteria and can serve as a feed additive to improve the reproductive performance of a female donkey and improve the growth performance of a donkey foal; the occurrence of donkey foal diarrhea is reduced, so that the economic benefit of donkey breeding is increased.

The present application provides a kind of complex probiotic paste, which combines Enterococcus faecalis, Saccharomyces cerevisiae and vitamin B 12 Producing lactic acid bacteria of canine source, adds prebiotics, flavoring substances and auxiliary substances such as paste matrix preparation.In the paste, the viable count of Enterococcus faecalis, Saccharomyces cerevisiae and lactic acid bacteria is 1-2:1-2:1-8;Paste matrix includes fructooligosaccharide 0.5-3%, psyllium husk powder 0.1-2%, goat milk powder 2-8%, chicken liver powder 1-5%, potassium sorbate 0.5-2%, xanthan gum 1-4%, and the balance is water.The above-mentioned complex probiotic paste can be stably stored for 56 days at 4 DEG C and normal temperature dry place, has good storage stability, good safety, and can adjust intestinal flora and increase the content of intestinal lactic acid bacteria.The above-mentioned complex probiotic paste has good palatability and oral tolerance for pets such as dogs and cats, and has good therapeutic effect on DSS-induced chronic enteritis diarrhea and vitamin B 12 Deficiency.

The invention discloses a walnut probiotic fermented beverage and a preparation method thereof. The beverage is prepared from walnut kernels as a preparation raw material through probiotic fermentation. The walnut kernels are used as preparation raw materials, the beverage is prepared through probiotic fermentation, and the beverage has the advantages of being good in taste, rich in nutrient substance, high in oxidation resistance and the like. The lactobacillus plantarum subsp. Plantarum RF17 and the lactobacillus rhamnosus GOLDGUT-M520 are combined for fermentation, so that the content of total flavonoids in the fermented beverage is increased, and the enterococcus faecalis 2010-JR-01 and the lactobacillus paracasei LCX25 are combined for fermentation, so that the content of total phenols in the fermented beverage is increased.

The invention relates to a seawater stichopus japonicus culture pond environment regulation and control method based on a bottom nourishing device and a mixed microbial agent and application. According to the method, the seawater stichopus japonicus culture pond is subjected to bottom nourishing operation, meanwhile, mixed microbial agents of rhodobacter sphaeroides, phosphorus phagocytosis bacillus, moraxella alpha and enterococcus faecalis are splashed, the water quality of the stichopus japonicus culture pond is improved, meanwhile, the immune function of stichopus japonicus is enhanced, and remarkable environmental and economic benefits are achieved.

The invention provides an enterococcus faecalis FS and application thereof. The enterococcus faecalis FS is preserved in the Guangdong Microbial Culture Collection Center on May 12, 2025, and the preservation number of the enterococcus faecalis FS is GDMCC (China General Microbiological Culture Collection Center) 66318. The novel enterococcus faecalis FS is separated from the intestinal tract of healthy adult largemouth bass, has good acid and alkali resistance, cholate resistance, acid production and antibacterial capacity, is good in biological safety and has no toxic or side effect on fishes. When the enterococcus faecalis FS provided by the invention is added into juvenile fish functional feed as a core component, not only can the growth performance and disease resistance of largemouth bass be improved, but also the use of antibiotics can be reduced, the environmental pollution can be reduced, the aquaculture industry can be promoted to develop towards a high-efficiency, environment-friendly and sustainable direction, and the enterococcus faecalis FS has important application value and market prospect.

The application discloses a cat oral cavity nursing probiotic composition and application thereof, and the probiotic composition comprises animal bifidobacterium and lactic acid bacteria, wherein the lactic acid bacteria is any one or a combination of multiple of salivarius, lactobacillus casei, enterococcus faecalis, lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus acidophilus and lactobacillus reuteri; compared with single probiotic, the probiotic composition has better effect on oral cavity odor removal, can effectively improve cat oral cavity source halitosis, and can also reduce dental plaque formation, relieve gingivitis and dental caries, and can be applied to preparation of a cat oral cavity odor cleaner, and has good application prospect.

The invention relates to the technical field of biology, in particular to a broad-spectrum bacteriophage lyase mutant, an extraction and purification reagent and a kit. The invention provides a broad-spectrum bacteriophage lyase mutant, an extraction and purification reagent and a kit. A broad-spectrum bacteriophage lyase mutant nucleic acid release liquid containing protein with an amino acid sequence as shown in SEQ ID NO: 1 is adopted; the compound can efficiently act on two important clinical drug-resistant bacteria, namely methicillin-resistant staphylococcus aureus MRSA and vancomycin-resistant enterococcus VRE, at the same time. Through unique molecular design and preparation process optimization, the lyase shows excellent synchronous wall breaking capacity, cell wall destruction can be completed in an extremely short time, and the nucleic acid release efficiency is remarkably superior to that of a traditional method.

The invention discloses a livestock and poultry breeding feed additive capable of reducing cost and improving effect. The livestock and poultry breeding feed additive is prepared from the following raw materials through compound fermentation: probiotic components: bacillus subtilis, clostridium butyricum and enterococcus; the enzyme preparation components comprise cellulose decomposition enzyme and xylanase; a fermentation medium: brown sugar and water; the traditional Chinese medicine auxiliary materials comprise wormwood, health sand and andrographis paniculata. The preparation method of the cost-reducing and effect-improving livestock and poultry breeding feed additive comprises the following steps of bacterium expanding culture, secondary fermentation and product treatment. The invention belongs to the technical field of livestock and poultry breeding, and particularly provides a livestock and poultry breeding feed additive and a preparation method thereof, and the livestock and poultry breeding feed additive can improve the intestinal microecological balance of livestock and poultry, improve the digestion and utilization rate of feed, enhance the immunity of livestock and poultry and reduce the breeding cost through scientific matching of probiotics, enzyme preparations, traditional Chinese medicine components and nutritional auxiliary materials.

The application belongs to the technical field of biotechnology, and provides application of an engineered Enterococcus faecalis capable of expressing human phenylalanine hydroxylase in a medicine for treating phenylketonuria, a plasmid with a human-derived phenylalanine hydroxylase gene after codon optimization of the Enterococcus faecalis is introduced into the Enterococcus faecalis, so that the human phenylalanine hydroxylase is expressed in the intestinal tract. Experimental results show that the human phenylalanine hydroxylase is successfully expressed in the Enterococcus faecalis through genetic engineering technology; the engineered Enterococcus faecalis can significantly metabolize phenylalanine in an in-vitro culture medium system; meanwhile, the engineered Enterococcus faecalis can significantly treat phenylketonuria, and can reduce plasma phenylalanine of a phenylketonuria suckling mouse by 46.8% after 4 weeks of treatment. It is indicated that the engineered Enterococcus faecalis has an outstanding therapeutic effect in preventing and / or treating phenylketonuria.

This invention discloses the application of a morel mycelium extract, belonging to the field of natural products. The morel mycelium extract is mainly composed of α-glucan, with a weight-average molecular weight of 10,000 kDa to 15,000 kDa, a number-average molecular weight of 10,000 kDa to 12,000 kDa, a polydispersity index of 1.0-1.3, and a glucose molar percentage of not less than 99%. The preparation method includes ultrasonic-assisted extraction, alcohol precipitation, enzymatic hydrolysis, dialysis, and ethanol gradient purification. The morel mycelium extract of this invention has the effect of regulating intestinal flora, specifically by improving… Enterococcus , Citrobacter and Bacillus Species abundance of genera, decreased Clostridium and Ruminococcus It has a high species abundance and is more easily utilized by the human gut microbiota than inulin, rapidly decomposing to produce short-chain fatty acids.

A method for preparing a drug for treating bovine cholecystitis belongs to the field of biological veterinary medicine. The present invention utilizes the original ecological pathogens of bovine cholecystitis, and further adds bovine blood, trace elements, and freeze-dried Escherichia coli powder, freeze-dried enterococcus powder, freeze-dried Pseudomonas aeruginosa powder, and lecithin. Weak ultrasound is used in a buffer solution to activate the activity of various pathogens, and in the intelligent cultivation process, the corresponding antimicrobial peptides and antimicrobial proteins are strongly induced in the body of the big-headed golden fly larvae. The antimicrobial peptides and antimicrobial proteins have the characteristics of high biological activity and broad spectrum of action. Clinical treatment results show that the drug prepared by the present invention has good efficacy and high efficiency. The production process of the present invention is easy to industrialize and control, the product consistency is good, energy is saved, and it is suitable for large-scale production.

This invention provides an in vitro cultured bezoar, its preparation method, and its application, belonging to the field of traditional Chinese medicine preparation technology. The preparation method of this invention involves obtaining a bile-resistant, high-β-glucuronidase-active *Escherichia coli* strain through bile gradient acclimation, which is then combined with *Enterococcus faecalis* to form a functional bacterial community. This community is then cultured in vitro through a three-stage process of proliferation, transformation, and crystallization, followed by gradient calcium ion-induced crystallization and vacuum freeze-drying to obtain in vitro cultured bezoar. This invention aims to solve the problems of low conversion efficiency, uneven crystallization, insufficient content of effective components, and unstable product quality in existing technologies. The obtained in vitro cultured bezoar product has significantly higher bilirubin and conjugated bile acid contents than the standards of the *Chinese Pharmacopoeia*, and the process is stable, controllable, and suitable for industrialization.

The invention discloses a broiler feed additive containing chlorella and application of the broiler feed additive. The additive contains chlorella; the feed also contains one or more of lactobacillus johnsonii, enterococcus faecalis, saccharomyces cerevisiae and bacillus amyloliquefaciens. By adding the additive disclosed by the invention into daily ration, the intestinal tissue form can be improved by regulating antioxidant and immune functions, so that the growth performance of the broiler chicken can be remarkably improved. In the additive, lactobacillus johnsonii BS15 and enterococcus faecalis YP-2 are combined for use, and saccharomyces cerevisiae WHY-7 and bacillus amyloliquefaciens TL are combined for use, so that the average weight of the broiler chicken is increased, the spleen index after LPS stimulation is reduced, and synergistic interaction is achieved.

The invention discloses an aromatic polyketone compound as well as a preparation method and application thereof. The compound GaB (3) has an effect of treating infection of multiple drug-resistant bacteria. The compound is obtained by being separated from a secondary metabolite of the symbiotic Streptomyces sp.QHH-9511 of licheniformis, and the compound 2, the compound 3, the compound 4 and the compound 5 are new compounds. According to the present invention, the research results show that the GaB can effectively sterilize a variety of drug-resistant bacteria (methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococcus, carbapenem-resistant acinetobacter baumannii, multi-drug-resistant pseudomonas aeruginosa, multi-drug-resistant salmonella, and the like); in addition, the compound has the characteristics of broad-spectrum sterilization, biofilm formation inhibition and low-frequency drug resistance, and can be combined with other antibiotics to cope with super bacteria. Mechanism research shows that GaB is combined with glucosamine-6-phosphate synthetase (GlmS) to prevent synthesis of cell walls so as to play an antibacterial role, and a plurality of constructed living body models show that the compound can effectively reduce the content of bacteria in a body and promote wound repair and has in-vivo medication safety. The research result shows that the GaB can be used as a safe and effective novel pilot natural active small molecule for resisting infection of multiple drug-resistant bacteria.

The application discloses a dog-derived Enterococcus faecalis ZJUIDS-D016 with anti-aging effect and application, the dog-derived Enterococcus faecalis ZJUIDS-D016 is classified and named as (Enterococcus faecalis) Enterococcus faecalis ), and the preservation number is CGMCC 27038.The strain of the application is isolated from the intestinal tract of a healthy dog, has acid resistance, bile salt resistance and broad-spectrum antibacterial activity, and is sensitive to chloramphenicol, erythromycin and penicillin.The strain of the application has strong host adaptability, can colonize in the intestinal tract of a dog and secrete organic acids and bacteriocins, significantly inhibits the proliferation of common pathogenic bacteria such as dog pathogenic escherichia coli, effectively improves the intestinal health of pets and delays aging, and is suitable for preparing pet probiotic products.

The invention provides a method for preparing ruminant biological feed through fermentation of sodium humate and probiotics, and relates to the field of solid feed fermention.The method comprises the steps that S1, candida utilis powder, lactobacillus plantarum liquid, enterococcus faecium liquid and beet molasses are stirred and mixed, and molasses bacterial liquid is obtained; s2, mixing the molasses bacterial liquid with the treated sodium humate and gelatinized starch urea according to a preparation ratio, and uniformly stirring; and S3, loading into an anaerobic sealed fermentation bag, and naturally fermenting in a greenhouse at the temperature of 25-35 DEG C for 30-48 hours. The biological feed which is rich in high-activity probiotics, high in protein and easy to digest and absorb by animals is prepared from byproducts such as sodium humate, molasses and the like by utilizing biological engineering, and can replace antibiotics in the feed.

The invention belongs to the technical field of feed processing, and particularly provides a preparation method of a bacterium-enzyme synergistic fermentation liquid feed, which comprises the following steps: adding water into feed raw materials, mixing, and adding a compound enzyme preparation for enzymolysis; the compound enzyme preparation comprises alkaline protease and xylanase; cooling the material liquid after enzymolysis, and inoculating a complex microbial inoculant for fermentation; the complex microbial inoculants comprise lactobacillus plantarum, enterococcus faecium and KM71 pichia pastoris; and after the fermentation is finished, sterilizing to obtain the bacterium-enzyme synergistic fermentation liquid feed. According to the preparation method provided by the invention, the pre-enzymolysis of macromolecular substances of the feed and the fermentation effect of microbial flora are cooperated, so that the content of nutrient substances in the feed is remarkably increased. Through sequential addition of an enzyme preparation and a microbial agent, two-stage precise temperature control fermentation and in-situ low-temperature pasteurization technology, fermentation is terminated on the premise of not destroying nutrition, and the problems of nutrition improvement, biogenic amine control and product shelf life are solved.

The present invention relates to a vaccine composition for prophylactic use against Streptococcus pneumoniae infections. This composition contains inactivated bacteria of Escherichia coli, Morganella morganii, Proteus mirabilis, Klebsiella pneumoniae and Enterococcus faecalis and preferably dextran and an aluminum compound as adjuvant.

The invention discloses an enterococcus faecalis bacteriophage with cross-species lysis capability and application of the enterococcus faecalis bacteriophage. The enterococcus faecalis bacteriophage is enterococcus faecalis bacteriophage EF38-P2, and the preservation number of the enterococcus faecalis bacteriophage is CCTCCM20251109; the enterococcus faecalis bacteriophage EF38-P2 is a strong bacteriophage, the enterococcus faecalis bacteriophage EF38-P2 can effectively remove and inhibit proliferation of enterococcus faecalis and enterococcus faecium and can be used for resisting infection of the enterococcus faecalis and the enterococcus faecium, the titer of the enterococcus faecalis bacteriophage EF38-P2 is 3.8 * 10 < 10 > PFU / mL, the enterococcus faecalis bacteriophage EF38-P2 has good activity under the action of a low-concentration chlorine-containing disinfectant, and the enterococcus faecalis bacteriophage EF38-P2 can be used for resisting infection of the enterococcus The enterococcus faecalis bacteriophage EF38-P2 has the advantages that the enterococcus faecalis bacteriophage EF38-P2 is resistant under the condition that the pH value is equal to 3-12, the titer is reduced by not more than 1 order of magnitude within 24 hours, meanwhile, the enterococcus faecalis bacteriophage EF38-P2 has tolerance under the condition that the pH value is equal to 3-12, the titer is reduced by not more than 4 order of magnitude within 96 hours, and the enterococcus faecalis bacteriophage EF38-P2 is good The enterococcus faecalis bacteriophage EF38-P2 can be prepared into medicines for preventing and treating enterococcus faecalis and enterococcus faecium, environment disinfectants, feed additives and the like for application.

A fermented natural substance having nitric oxide metabolites fixated and stabilized therein is produced by a process including adding a fermentative strain to nitrogen-containing natural substance and carrying out fermentation by a batch fermentation process. The nitrogen-containing natural substance is at least one selected from the group consisting of lettuce, Sedum sarmentosum, spinach, blueberry, dandelion, pomegranate, cabbage, garlic, Morinda citrifolia, onion, soybean, bean sprout, mulberry leaf, Momordica charantia, Rubus coreanus, Houttuynia cortada, aronia, Humulus japonicus, Coptis chinensis, hijiki, hooker chive, mushrooms, calamus, wild spinach (Seomcho), Chinese cabbage, kelp, apple, mugwort, orange, salmon testis, abalone shell, shellfish shell, cricket, silkworm, Rehmannia glutinosa, and Shipjeondaebo-tang. to Bacillus sp., Bifidobacterium sp., Enterococcus sp., or Lactobacillus sp.

This invention relates to the field of microbial technology, and more particularly to *Providens skeletalus*, a high-lysine-producing strain, a compound microbial agent, and its applications. This invention isolates *Providens skeletalus* from the intestines of black soldier fly larvae. This strain exhibits high amino acid production, which can improve feed quality and reduce dependence on high-value protein sources. A combination of *Providens skeletalus* with *Enterococcus faecalis* and *Bacillus licheniformis* in a 1:1:1 ratio demonstrates excellent synergistic effects, efficiently degrading organic waste and promoting black soldier fly growth or the synthesis of microbial proteins rich in essential amino acids. This combination has significant application value in the field of organic waste resource utilization.

The invention relates to the technical field of microbial feed additives, and discloses a feed lactic acid bacteria additive and a preparation method thereof.The preparation method comprises the steps that lactobacillus plantarum and / or enterococcus faecalis are / is used as strains for activation and expanding culture, agricultural and sideline product waste is used as a substrate for liquid-state deep fermentation, and mature fermentation liquor is obtained; mixing the mature fermentation liquor with a composite protective agent containing double essential oil, chitosan, theabrownin and porous silicon dioxide, granulating, and pre-drying at low temperature to obtain a semi-dry particle core; and coating with kappa-carrageenan / konjac glucomannan / phytosterol ester composite gel, drying and packaging to obtain a finished product. The double essential oils, the chitosan and the theabrownin are combined to form a full-spectrum active antibacterial system; the elastic composite gel outer layer realizes dual protection of liquid water and water vapor; porous silicon dioxide actively maintains low water activity of particles to prevent lactic acid bacteria from being recovered in advance; the product has the advantages of high viable count, strong heat and acid resistance and excellent storage stability.

The invention relates to the technical field of poultry immunology, in particular to a universal riemerella anatipestifer monoclonal antibody, a detection kit and application of the universal riemerella anatipestifer monoclonal antibody. Specifically, the invention successfully designs the universal riemerella anatipestifer monoclonal antibody, and the detection kit developed based on the monoclonal antibody can rapidly detect the level of the riemerella anatipestifer serum antibody in a sample, and has high sensitivity and strong specificity. The riemerella anatipestifer strain has no cross reactivity with avian enterococcus faecalis, avian salmonella typhimurium, avian escherichia coli and avian salmonella enteritidis, so that the riemerella anatipestifer strain can be applied to rapid screening, epidemiological monitoring and vaccine immune effect evaluation of riemerella anatipestifer infection, and has a wide application prospect.

The invention discloses a single-bacterium fermentation compounding method of cattle feed, and relates to the technical field of feed fermentation. Comprising the following steps: preparing a single-bacterium fermentation bacterial material, the single-bacterium fermentation bacterial material comprises 42.25-47.25 parts of wheat bran, 19-21 parts of soybean meal, 14.25-15.75 parts of corn flour, 19-21 parts of rice hull powder and 0.003-0.005 part of a bacterial liquid, and strains used by the bacterial liquid comprise six probiotics including saccharomyces cerevisiae, bacillus subtilis, bacillus licheniformis, lactobacillus plantarum, enterococcus faecalis and lactobacillus acidophilus; the six probiotics are subjected to independent solid fermentation, the prepared fermentation fungus materials are compounded in proportion to obtain a compound fermentation fungus material, and the compound fermentation fungus material is fed according to cows with different weights. Through the technical route of single-bacterium independent fermentation, the problem of mutual inhibition generated during compound fermentation of various strains is avoided, high-density propagation of the strains in the fermentation process is guaranteed, the compounded feed can meet the nutritional requirements of cattle in different stages, and the yield of the cattle is improved. The feed digestion and absorption rate is effectively improved, the cattle immunity is enhanced, and the growth performance is promoted.