153 results about "Enterococcus" patented technology

The invention relates to the technical field of animal feed preparation, in particular to a functional feed additive for ruminants and a preparation method of the functional feed additive for the ruminants, and the functional feed additive for the ruminants is characterized by consisting of the following components in percentage by mass: probiotics which are saccharomycetes, bacillus subtilis and enterococcus faecalis; an enzyme preparation, xylanase and protease; prebiotics are added; a microcapsule coating material; a binder; the balance is a carrier; the probiotics and the enzyme preparation are wrapped in the chitosan / sodium alginate composite film through a microcapsule coating technology, adverse factors such as temperature, humidity and illumination in the production, transportation and storage process can be effectively isolated, meanwhile, in the rumen low-pH, strong-protease and complex-microorganism environment, the coating layer can slowly release and protect active ingredients, and the effect of preventing and treating the probiotics and the enzyme preparation is achieved. Effective content and activity during final feeding are ensured, consistent growth promotion can be kept in different varieties, weights and feeding stages, and production benefits and feeding stability are improved.

The invention relates to the technical field of biomineralization, and particularly discloses an alkali-resistant urease-producing microbial flora for biomineralization of construction joints and a preparation method of the alkali-resistant urease-producing microbial flora. The microbial flora disclosed by the invention is prepared from the following strains with relative abundance: 4.4 to 5.2 percent of bacillus, 0.39 to 0.46 percent of enterococcus, 4.8 to 5.7 percent of alcaligenes, 0.50 to 0.58 percent of cellulomonas, 22.4 to 24.2 percent of lysobacter, 1.7 to 2.4 percent of pseudomonas, 10.8 to 11.9 percent of Savagea, 15.1 to 16.2 percent of sarcina, 30.8 to 32.2 percent of chloridomonas and 0.41 to 0.48 percent of bacillus. The alkali-resistant microbial compound flora with high urease activity in the limited oxygen and normal environment can effectively overcome the defects that urease microorganisms are low in mineralization efficiency under the anoxic condition, and the strength of an obtained consolidated body is not high.

The invention belongs to the technical field of microorganisms, and discloses a Lactobacillus paracasei B20 for inhibiting melanin from a plateau area and application of the Lactobacillus paracasei B20, the Lactobacillus paracasei B20 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32434, and the preservation date is October 31, 2024. Metabolite of the lactobacillus paracasei B20 comprises phenylalanine, p-hydroxycinnamic acid and the like, has strong tyrosinase inhibition effect and anti-ultraviolet ability, and can significantly reduce generation of melanin in skin. In addition, the lactobacillus paracasei B20 also has relatively strong oxidation resistance, has good tolerance in artificial gastric juice and artificial intestinal juice, and can smoothly reach the intestinal tract of a human body. In addition, the lactobacillus paracasei B20 has a relatively obvious growth inhibition effect on skin conditioned pathogens such as staphylococcus aureus and enterococcus faecalis.

The invention discloses a preparation method of nano ozone water and application of the nano ozone water in killing food-borne pathogenic bacteria, and belongs to the technical field of bactericides. The nano ozone water is prepared by utilizing the ultra-fine bubble generator and the ozone generator, and the prepared nano ozone water is high in stability, not easy to decompose and high in practicability. The nano ozone water can achieve an effective killing effect on escherichia coli, salmonella, enterococcus, campylobacter and clinical multi-drug-resistant bacteria thereof, and a new resource is provided for preparing a safe and reliable antibacterial product.

The invention discloses application of vanillylamine in preparation of a preparation for preventing and treating rice stem borers, and belongs to the technical field of microbial pesticides. The invention finds that the enterococcus metabolite vanillylamine, namely 4-(aminomethyl)-2-methoxyphenol, has insecticidal activity on rice stem borers, and particularly has excellent insecticidal activity on chilo suppressalis, so that a new biocontrol resource is provided for prevention and treatment of the rice pest chilo suppressalis.

Compositions comprising 5. salivarius strain SALI-10 and / or salivarius 10 peptide are taught. Also taught are methods of treating bacterial infections comprising administering a pharmaceutically acceptable amount of the composition to the patient with a bacterial infection caused by a pathogen from, for example, multi-drug resistant (MDR) Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus dysagalactiae, vancomycin-resistant Enterococcus faecium, Porphyromonas gingivalis, Tannerella forsythia, and MDR Enterococcus faecalis.

The invention relates to the technical field of microorganisms, in particular to enterococcus faecalis capable of degrading uric acid and application of the enterococcus faecalis, the enterococcus faecalis is named as Enterococcus faecalis SX3, and the preservation number of the enterococcus faecalis is CCTCC NO: M 2025248. The enterococcus faecalis has the advantages of being wide in antibacterial spectrum, rapid in growth, high in stress resistance, high in biological safety and the like, and important application value is provided for formulating a scheme for degrading uric acid.

The invention discloses a composition for treating hyperuricemia nephropathy and application thereof, and belongs to the field of biological medicines.The composition is prepared from, by weight, 28-32 parts of rhizoma smilacis glabrae, 28-32 parts of yam rhizome, 13-17 parts of radix clematidis, 8-12 parts of peach kernels, 8-12 parts of flos carthami, 8-12 parts of herba lycopi, 18-22 parts of rhizoma alismatis, 28-32 parts of raw coix seeds, 28-32 parts of semen plantaginis, 13-17 parts of rhizoma atractylodis and 13-17 parts of pseudobulbus cremastrae seu pleiones. The composition can obviously reduce the uric acid level of a mouse with high uric acid nephropathy and improve kidney injury; meanwhile, intestinal flora of mice with the hyperuricemia nephropathy can be remarkably remodeled, abundance of beneficial bacteria Ackerman's bacteria is up-regulated, abundance of opportunistic pathogenic bacteria such as escherichia coli-shigella, desulfurization vibrio and enterococcus is down-regulated, and important significance is achieved for research and drug development of the hyperuricemia nephropathy.

The invention discloses a stichopus japonicus fry cultivation method, and belongs to the technical field of stichopus japonicus fry cultivation. Comprising the following steps: splashing a fermented feed containing a bacillus subtilis fermentation liquid and a bacterial liquid containing an enterococcus faecalis fermentation liquid and a lactobacillus casei fermentation liquid into a culture pond of which the pond bottom is paved with a polyethylene corrugated plate substratum; the fermented feed and the bacterial liquid are continuously splashed until the water quality in the culture pond is clear and transparent, the feed on the corrugated plate is fluffy and is in a short villus shape, and a stable beneficial bacterium phase is formed; peeling off the juvenile sea cucumbers on the 40th day after metamorphosis and attachment, distributing the juvenile sea cucumbers into a cultivation pond for cultivation, and feeding fermented feed and bacterial liquid every day. The method is applied to the aspect of stichopus japonicus fry cultivation, the problems that in existing stichopus japonicus fry production, diseases occur frequently, most fry breeding fields face poor fry quality, and the yield is low are solved, a stable cultivation environment dominated by beneficial bacteria is built, external multi-dimensional disease sources are buffered, blocked and eliminated continuously, and the survival rate of the stichopus japonicus fry is increased. The stichopus japonicus seedlings can grow in the environment without stress.

The invention relates to a composite microbial inoculant and an evaluation method for the antibacterial effect thereof. The method optimizes the in-vitro culture mode of the microbial agent compounded by aerobic bacteria and anaerobic bacteria by simulating the intestinal environment of probiotics in an animal body, and adopts a facultative anaerobic culture method for the microbial compound agent, namely, the outer layer of a sealing film of a triangular flask is wrapped with four layers of preservative films, and the neck of the triangular flask is tightened by a rubber band. The facultative anaerobic environment of aerobic bacillus and facultative anaerobic enterococcus faecalis is considered in the microbial composite inoculant, in the subsequent oxford cup bacteriostasis evaluation experiment, the experiment result with the accurate bacteriostasis effect and good parallelism can be obtained, and the action effect of bacillus and enterococcus faecalis composite bacteria can be efficiently and scientifically evaluated.

The microorganism strain Pediococcus acidilactici TAK 589 CoccobEst is a new antimicrobial and antioxidant probiotic, CoccobEst inhibits the growth of the enteropathogens L. monocytogenes, E. coli, S. enteritidis, S. typhimurium, S. sonnei, S. aureus, E. faecalis and C. sakazakii, but does not inhibit lactobacilli of natural intestinal microbiota. The strain's antimicrobial properties are enhanced by periplasmic peptidoglycan hydrolases with proteolytic activity. CoccobEst is used as a zootechnieai feed additive to prevent bacterial infections in the gastrointestinal tract of farm animals and pets and to prevent and reduce diarrhea. CoccobEst has highly active antioxidant defense system, increasing the redox activity of glutathione and reducing oxidative stress in cells. Therefore, CoccobEst is used as a food supplement or functional food for humans to prevent bacterial infections of the gastrointestinal tract and to prevent and reduce oxidative stress. In addition, CoccobEst is used as a starter culture in fermentation of vegetables.

The invention discloses a method for fermenting vinasse feed through mixed bacteria, and belongs to the field of microbial fermentation. The invention firstly provides a microbial agent for increasing the content of crude protein in fermented vinasse. The microbial agent contains a bacillus subtilis liquid, a corynebacterium glutamicum liquid, a saccharomyces cerevisiae liquid, an enterococcus faecalis liquid and a bifidobacterium animalis liquid. The invention also provides an application of the microbial agent in preparation of a fermented vinasse feed. The contents of crude protein, crude fat and amino acid in the fermented vinasse feed are increased. Through mixed fermentation, the content of amino acid in the vinasse is increased, the content of lysine is increased by 108.33%, and the content of threonine is increased by 59.09%.

An object of the present invention is to provide a new lytic agent capable of lysing Enterococcus bacteria. Provided is a lytic agent for Enterococcus bacteria, consisting of a lytic enzyme or an active fragment thereof, the lytic enzyme comprising: (a) the amino acid sequence shown in SEQ ID NO: 1, (b) an amino acid sequence in which one or multiple amino acids are added, deleted, and / or substituted in the amino acid sequence shown in SEQ ID NO: 1, or (c) an amino acid sequence having a sequence identity of 90% or more with the amino acid sequence shown in SEQ ID NO: 1.

The invention relates to the technical field of microorganisms, in particular to mixed bacteria fermentation liquor as well as a preparation method and application thereof. Bacterial strains in the mixed fermentation liquor comprise clostridium butyricum, enterococcus faecium and bacillus. The preparation method of the mixed bacteria fermentation liquor comprises the following steps: culturing the strains in the mixed bacteria fermentation liquor in a mixed bacteria fermentation culture medium to obtain the mixed bacteria fermentation liquor; wherein the bacterial strains comprise clostridium butyricum, enterococcus faecium and bacillus. According to the mixed bacterium fermentation liquor and the preparation method and application thereof, coexisting culture of donkey source enterococcus faecium, donkey source bacillus and donkey source clostridium butyricum can be achieved through the mixed bacterium fermentation liquor, and meanwhile the mixed bacterium fermentation liquor has the excellent effects of producing compound enzyme and inhibiting pathogenic bacteria and can serve as a feed additive to improve the reproductive performance of a female donkey and improve the growth performance of a donkey foal; the occurrence of donkey foal diarrhea is reduced, so that the economic benefit of donkey breeding is increased.

The present application provides a kind of complex probiotic paste, which combines Enterococcus faecalis, Saccharomyces cerevisiae and vitamin B 12 Producing lactic acid bacteria of canine source, adds prebiotics, flavoring substances and auxiliary substances such as paste matrix preparation.In the paste, the viable count of Enterococcus faecalis, Saccharomyces cerevisiae and lactic acid bacteria is 1-2:1-2:1-8;Paste matrix includes fructooligosaccharide 0.5-3%, psyllium husk powder 0.1-2%, goat milk powder 2-8%, chicken liver powder 1-5%, potassium sorbate 0.5-2%, xanthan gum 1-4%, and the balance is water.The above-mentioned complex probiotic paste can be stably stored for 56 days at 4 DEG C and normal temperature dry place, has good storage stability, good safety, and can adjust intestinal flora and increase the content of intestinal lactic acid bacteria.The above-mentioned complex probiotic paste has good palatability and oral tolerance for pets such as dogs and cats, and has good therapeutic effect on DSS-induced chronic enteritis diarrhea and vitamin B 12 Deficiency.

The invention discloses a walnut probiotic fermented beverage and a preparation method thereof. The beverage is prepared from walnut kernels as a preparation raw material through probiotic fermentation. The walnut kernels are used as preparation raw materials, the beverage is prepared through probiotic fermentation, and the beverage has the advantages of being good in taste, rich in nutrient substance, high in oxidation resistance and the like. The lactobacillus plantarum subsp. Plantarum RF17 and the lactobacillus rhamnosus GOLDGUT-M520 are combined for fermentation, so that the content of total flavonoids in the fermented beverage is increased, and the enterococcus faecalis 2010-JR-01 and the lactobacillus paracasei LCX25 are combined for fermentation, so that the content of total phenols in the fermented beverage is increased.

The present invention relates to a method for treating conditions associated with weight loss in emaciation and cachexia with a probiotic composition and the use of a probiotic composition in the treatment of conditions associated with weight loss in emaciation and cachexia. The present invention provides a method of treating a condition associated with weight loss, the method comprising administering to a subject considered in need of such treatment a probiotic composition wherein the composition comprises at least two bacteria, each preferably selected from a different genus, the genus is preferably selected from the group consisting of Lactobacillus genus, Enterococcus genus and Bifidobacterium genus. The present invention also provides a probiotic composition for use in the treatment of a subject suffering from a condition associated with weight loss wherein the composition comprises at least two bacteria each preferably selected from a different genus, preferably selected from the group consisting of Lactobacillus genus, Enterococcus genus and Bifidobacterium genus.

The invention features E. faecalis phage-derived antimicrobial agent (efagins). The invention also features compositions and methods of using the efagins to treat bacterial infections.

The invention relates to the field of microorganisms, in particular to application of a microbial strain in preparation of a medicine for preventing or treating metabolic diseases, the microbial strain belongs to Enterococcus canini, and the metabolic diseases comprise diabetes, obesity, obesity-related diseases and metabolic syndromes. According to the present invention, with the application of the Enterococcus canintestini, the target spots GPR120 and MC4R for the treatment of obesity diabetes can be significantly activated; the weight, fasting blood glucose, area under a glucose tolerance curve and area under an insulin tolerance curve of obese mice can be reduced, the content of free fatty acid in plasma, total cholesterol in liver and low-density lipoprotein-cholesterol in liver can be reduced, and the content of glucagon-like peptide-1 in plasma and the content of high-density lipoprotein-cholesterol in liver can be increased.

The invention belongs to the technical field of biology, and particularly relates to an antibacterial peptide sourced from symbiotic methane-oxidizing bacteria in deep sea and application of the antibacterial peptide. The amino acid sequence of the antibacterial peptide is shown as SEQ ID NO. 1. The antibacterial peptide provided by the invention is a novel antibacterial peptide, has broad-spectrum antibacterial activity on a variety of gram-negative bacteria and gram-positive bacteria pathogens, such as pseudomonas aeruginosa, listeria monocytogenes, enterococcus faecalis and various vibrios, and has the minimum inhibitory concentration of 8.13-16.25 [mu] M. In addition, the antibacterial peptide has relatively weak hemolytic activity and relatively low cytotoxicity. The antibacterial peptide provided by the invention can replace antibiotics, is applied to prevention and treatment of various bacterial diseases, and is used as a safe and stable food additive or cosmetic preservative candidate substance.

The invention discloses a special bioactive soft granular feed for crayfish breeding and a preparation process thereof, comprising the following raw materials in parts by weight: 2 to 8 parts of fish meal, 15 to 25 parts of puffed soybean meal, 5 to 11 parts of rapeseed meal, 6 to 12 parts of wheat bran, 2.5 to 5.5 parts of compound lactic acid bacteria (Lactobacillus plantarum, Lactobacillus acidophilus, Enterococcus faecalis), 3 to 7 parts of shrimp meal, 1 to 3 parts of calcium dihydrogen phosphate, 10 to 30 parts of modified cassava starch, 0.5 to 1.5 parts of fish oil, 0.5 to 1.5 parts of premix, 0.5 to 1.5 parts of vegetable oil, and 15 to 33 parts of fresh maggot paste. The formula of the present invention is more reasonable, nutritionally comprehensive, and each component can be better matched to meet the nutritional needs of crayfish, and can better promote the growth of crayfish, especially in that modified cassava starch and fresh maggot paste are added to the formula, and granulation is carried out at low temperature or room temperature, which can retain the biological activity in the feed to the greatest extent, improve the proportion of animal protein, and be more conducive to crayfish absorption. At the same time, the feed solubility rate is lower, the stability in water is stronger, the feed utilization rate is effectively improved, and the cost of protein raw materials is reduced.

The invention relates to a seawater stichopus japonicus culture pond environment regulation and control method based on a bottom nourishing device and a mixed microbial agent and application. According to the method, the seawater stichopus japonicus culture pond is subjected to bottom nourishing operation, meanwhile, mixed microbial agents of rhodobacter sphaeroides, phosphorus phagocytosis bacillus, moraxella alpha and enterococcus faecalis are splashed, the water quality of the stichopus japonicus culture pond is improved, meanwhile, the immune function of stichopus japonicus is enhanced, and remarkable environmental and economic benefits are achieved.

The invention provides an enterococcus faecalis FS and application thereof. The enterococcus faecalis FS is preserved in the Guangdong Microbial Culture Collection Center on May 12, 2025, and the preservation number of the enterococcus faecalis FS is GDMCC (China General Microbiological Culture Collection Center) 66318. The novel enterococcus faecalis FS is separated from the intestinal tract of healthy adult largemouth bass, has good acid and alkali resistance, cholate resistance, acid production and antibacterial capacity, is good in biological safety and has no toxic or side effect on fishes. When the enterococcus faecalis FS provided by the invention is added into juvenile fish functional feed as a core component, not only can the growth performance and disease resistance of largemouth bass be improved, but also the use of antibiotics can be reduced, the environmental pollution can be reduced, the aquaculture industry can be promoted to develop towards a high-efficiency, environment-friendly and sustainable direction, and the enterococcus faecalis FS has important application value and market prospect.

The application discloses a cat oral cavity nursing probiotic composition and application thereof, and the probiotic composition comprises animal bifidobacterium and lactic acid bacteria, wherein the lactic acid bacteria is any one or a combination of multiple of salivarius, lactobacillus casei, enterococcus faecalis, lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus acidophilus and lactobacillus reuteri; compared with single probiotic, the probiotic composition has better effect on oral cavity odor removal, can effectively improve cat oral cavity source halitosis, and can also reduce dental plaque formation, relieve gingivitis and dental caries, and can be applied to preparation of a cat oral cavity odor cleaner, and has good application prospect.

The invention relates to the technical field of textile detection, in particular to a method for detecting enterococcus faecalis in textiles, which comprises the following steps: S1, sample preparation: selecting a weighing method, a multi-point sampling elution method or a cotton swab smearing method according to the types of the textiles to prepare a sample homogenate; step S2, dilution and inoculation: performing 10-time series dilution on the sample homogenate, selecting a sample with 2-3 continuous dilution degrees, inoculating the sample to a bile aesculin sodium azide agar culture medium, and culturing at 36 + / -1 DEG C for 24-48 hours. According to the method, the detection technology of enterococcus faecalis in the textile is innovatively adopted, and the pollution condition of enterococcus faecalis in the textile is effectively monitored; the method has the following remarkable advantages that operation is easy and convenient, cost is low, complex instruments and equipment are not needed, the method is suitable for conventional detection of a primary laboratory, and the technology has outstanding originality and practicability in the aspect of improving the biological safety prevention and control capacity of the textile.

The invention discloses enterococcus faecalis for degrading uric acid in an anaerobic environment and application of the enterococcus faecalis, and belongs to the technical field of microorganisms. The strain is preserved in the China General Microbiological Culture Collection Center of Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing on February 19, 2025, and the preservation number is CGMCC No.33565. The enterococcus faecalis with the uric acid reducing effect is obtained by separating and culturing healthy human intestinal tracts, and the enterococcus faecalis can directly metabolize uric acid in an anaerobic environment and has an excellent uric acid degrading effect.

The invention relates to the technical field of biology, in particular to a broad-spectrum bacteriophage lyase mutant, an extraction and purification reagent and a kit. The invention provides a broad-spectrum bacteriophage lyase mutant, an extraction and purification reagent and a kit. A broad-spectrum bacteriophage lyase mutant nucleic acid release liquid containing protein with an amino acid sequence as shown in SEQ ID NO: 1 is adopted; the compound can efficiently act on two important clinical drug-resistant bacteria, namely methicillin-resistant staphylococcus aureus MRSA and vancomycin-resistant enterococcus VRE, at the same time. Through unique molecular design and preparation process optimization, the lyase shows excellent synchronous wall breaking capacity, cell wall destruction can be completed in an extremely short time, and the nucleic acid release efficiency is remarkably superior to that of a traditional method.

The invention discloses a livestock and poultry breeding feed additive capable of reducing cost and improving effect. The livestock and poultry breeding feed additive is prepared from the following raw materials through compound fermentation: probiotic components: bacillus subtilis, clostridium butyricum and enterococcus; the enzyme preparation components comprise cellulose decomposition enzyme and xylanase; a fermentation medium: brown sugar and water; the traditional Chinese medicine auxiliary materials comprise wormwood, health sand and andrographis paniculata. The preparation method of the cost-reducing and effect-improving livestock and poultry breeding feed additive comprises the following steps of bacterium expanding culture, secondary fermentation and product treatment. The invention belongs to the technical field of livestock and poultry breeding, and particularly provides a livestock and poultry breeding feed additive and a preparation method thereof, and the livestock and poultry breeding feed additive can improve the intestinal microecological balance of livestock and poultry, improve the digestion and utilization rate of feed, enhance the immunity of livestock and poultry and reduce the breeding cost through scientific matching of probiotics, enzyme preparations, traditional Chinese medicine components and nutritional auxiliary materials.

The present invention belongs to the field of biotechnology and provides a strain of Enterococcus faecalis with a preservation number of CGMCC No. 29087, which is characterized in that the Enterococcus faecalis can secrete bacteriocins that inhibit Vibrio parahaemolyticus, Staphylococcus aureus and Escherichia coli. The present invention also provides the application of the Enterococcus faecalis and the same in aquatic fermented feed and laying hen premix, which can effectively improve the utilization rate of feed and the growth and production performance of animals.

The application belongs to the technical field of biotechnology, and provides application of an engineered Enterococcus faecalis capable of expressing human phenylalanine hydroxylase in a medicine for treating phenylketonuria, a plasmid with a human-derived phenylalanine hydroxylase gene after codon optimization of the Enterococcus faecalis is introduced into the Enterococcus faecalis, so that the human phenylalanine hydroxylase is expressed in the intestinal tract. Experimental results show that the human phenylalanine hydroxylase is successfully expressed in the Enterococcus faecalis through genetic engineering technology; the engineered Enterococcus faecalis can significantly metabolize phenylalanine in an in-vitro culture medium system; meanwhile, the engineered Enterococcus faecalis can significantly treat phenylketonuria, and can reduce plasma phenylalanine of a phenylketonuria suckling mouse by 46.8% after 4 weeks of treatment. It is indicated that the engineered Enterococcus faecalis has an outstanding therapeutic effect in preventing and / or treating phenylketonuria.