175 results about "Enterococcus" patented technology

The invention relates to the technical field of animal feed preparation, in particular to a functional feed additive for ruminants and a preparation method of the functional feed additive for the ruminants, and the functional feed additive for the ruminants is characterized by consisting of the following components in percentage by mass: probiotics which are saccharomycetes, bacillus subtilis and enterococcus faecalis; an enzyme preparation, xylanase and protease; prebiotics are added; a microcapsule coating material; a binder; the balance is a carrier; the probiotics and the enzyme preparation are wrapped in the chitosan / sodium alginate composite film through a microcapsule coating technology, adverse factors such as temperature, humidity and illumination in the production, transportation and storage process can be effectively isolated, meanwhile, in the rumen low-pH, strong-protease and complex-microorganism environment, the coating layer can slowly release and protect active ingredients, and the effect of preventing and treating the probiotics and the enzyme preparation is achieved. Effective content and activity during final feeding are ensured, consistent growth promotion can be kept in different varieties, weights and feeding stages, and production benefits and feeding stability are improved.

The invention discloses an antibacterial peptide SAMP4 or a derivative thereof, a pharmaceutical composition and application, and belongs to the technical field of biology, the amino acid sequence of the antibacterial peptide SAMP4 is as shown in SEQ ID NO: 1, and the antibacterial peptide SAMP4 has a broad-spectrum antibacterial effect and can be used for preparing antibacterial peptide SAMP4. The compound has a strong bacteriostatic effect on various gram-positive bacteria such as staphylococcus aureus, enterococcus and streptococcus and various gram-negative bacteria such as escherichia coli, acinetobacter baumannii and pseudomonas aeruginosa, and is strong in antibacterial activity, wide in antibacterial spectrum and low in toxicity. The invention also provides a derivative and a salt of the antibacterial peptide SAMP4 and application of the antibacterial peptide SAMP4 and the derivative and the salt of the antibacterial peptide SAMP4 to research, development and preparation of various antibacterial products or / and antibacterial drugs and treatment of bacterial infectious diseases, and the antibacterial peptide SAMP4 and the derivative and the salt of the antibacterial peptide SAMP4 are remarkable in advantages and wide in application prospect.

The invention relates to the technical field of biomineralization, and particularly discloses an alkali-resistant urease-producing microbial flora for biomineralization of construction joints and a preparation method of the alkali-resistant urease-producing microbial flora. The microbial flora disclosed by the invention is prepared from the following strains with relative abundance: 4.4 to 5.2 percent of bacillus, 0.39 to 0.46 percent of enterococcus, 4.8 to 5.7 percent of alcaligenes, 0.50 to 0.58 percent of cellulomonas, 22.4 to 24.2 percent of lysobacter, 1.7 to 2.4 percent of pseudomonas, 10.8 to 11.9 percent of Savagea, 15.1 to 16.2 percent of sarcina, 30.8 to 32.2 percent of chloridomonas and 0.41 to 0.48 percent of bacillus. The alkali-resistant microbial compound flora with high urease activity in the limited oxygen and normal environment can effectively overcome the defects that urease microorganisms are low in mineralization efficiency under the anoxic condition, and the strength of an obtained consolidated body is not high.

The invention is applicable to the technical field of polypeptides, and provides a novel antibacterial peptide GG25 and application thereof, and the amino acid sequence of the antibacterial peptide GG25 is as shown in SEQ ID No.1. The molecular weight of the novel antibacterial peptide GG25 is 2890.52 Da, and the novel antibacterial peptide GG25 has antibacterial activity on escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, enterococcus faecalis, salmonella typhimurium, methicillin-resistant staphylococcus aureus, staphylococcus aureus, staphylococcus epidermidis, listeria monocytogenes and bacillus subtilis; the hemolytic activity is low, the cytotoxicity is small, and the escherichia coli and methicillin-resistant staphylococcus aureus are not easily induced to generate drug resistance; in addition, the gelatin chitosan food coating containing GG25 also has a good beef preservation effect, and can be applied to the fields of food, animal husbandry, medicine and the like.

The invention belongs to the technical field of biology, and provides enterococcus faecalis, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.29087. The enterococcus faecalis is characterized in that the enterococcus faecalis can secrete bacteriocin for inhibiting vibrio parahaemolyticus, staphylococcus aureus and escherichia coli. The invention also provides the enterococcus faecalis and application thereof in an aquatic product fermented feed and a laying hen premix, and the enterococcus faecalis can effectively improve the utilization rate of the feed and the growth and production performance of animals.

The invention belongs to the technical field of microorganisms, and discloses a Lactobacillus paracasei B20 for inhibiting melanin from a plateau area and application of the Lactobacillus paracasei B20, the Lactobacillus paracasei B20 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32434, and the preservation date is October 31, 2024. Metabolite of the lactobacillus paracasei B20 comprises phenylalanine, p-hydroxycinnamic acid and the like, has strong tyrosinase inhibition effect and anti-ultraviolet ability, and can significantly reduce generation of melanin in skin. In addition, the lactobacillus paracasei B20 also has relatively strong oxidation resistance, has good tolerance in artificial gastric juice and artificial intestinal juice, and can smoothly reach the intestinal tract of a human body. In addition, the lactobacillus paracasei B20 has a relatively obvious growth inhibition effect on skin conditioned pathogens such as staphylococcus aureus and enterococcus faecalis.

The present disclosure provides a composition and methods for preventing, reducing, or eliminating a mastitis infection in a bovine animal. The composition includes an inhibitory composition in an amount effective to inhibit the growth of infectious mastitis pathogens in a mammary system of the bovine animal, where the inhibitory composition includes a microbial composition or an extract of one or more microbes of a mammary system-associated microbiota of healthy bovines, including Anaerocolumna aminovalerica, Bacillus glycinifermentans, Bacillus licheniformis, Bacillus pumilis, Bacillus sonorensis, Bacillus_AY sp002271785, Bacillus butanolivorans, Brevibacillus borstelensis, Brevibacterium epidermis, Clostridium aerotolerans, Corynebacterium sp., Corynebacterium xerosis, Cutibacterium acnes, Cytobacillus kochii, Dietzia sp., Enterococcus aquimarinus, Enterococcus faecalis, Enterococcus_A pseudoavium, Facklamia sp., Facklamia tabacinasalis, Hungatella, Lacrimispora sp., Lacrimispora algidixylanolytica, Metabacillus niabensis, Micrococcus luteus, Paenibacillus pabuli, Paraclostridium bifermentans, Peribacillus simplex, Populibacterium corticicola, Timonella sp., Tissierella creatinini, and Weissella jogaejeotgali, or any combinations thereof.

The invention discloses a preparation method of nano ozone water and application of the nano ozone water in killing food-borne pathogenic bacteria, and belongs to the technical field of bactericides. The nano ozone water is prepared by utilizing the ultra-fine bubble generator and the ozone generator, and the prepared nano ozone water is high in stability, not easy to decompose and high in practicability. The nano ozone water can achieve an effective killing effect on escherichia coli, salmonella, enterococcus, campylobacter and clinical multi-drug-resistant bacteria thereof, and a new resource is provided for preparing a safe and reliable antibacterial product.

The invention discloses application of vanillylamine in preparation of a preparation for preventing and treating rice stem borers, and belongs to the technical field of microbial pesticides. The invention finds that the enterococcus metabolite vanillylamine, namely 4-(aminomethyl)-2-methoxyphenol, has insecticidal activity on rice stem borers, and particularly has excellent insecticidal activity on chilo suppressalis, so that a new biocontrol resource is provided for prevention and treatment of the rice pest chilo suppressalis.

Compositions comprising 5. salivarius strain SALI-10 and / or salivarius 10 peptide are taught. Also taught are methods of treating bacterial infections comprising administering a pharmaceutically acceptable amount of the composition to the patient with a bacterial infection caused by a pathogen from, for example, multi-drug resistant (MDR) Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus dysagalactiae, vancomycin-resistant Enterococcus faecium, Porphyromonas gingivalis, Tannerella forsythia, and MDR Enterococcus faecalis.

The invention relates to the technical field of microorganisms, in particular to enterococcus faecalis capable of degrading uric acid and application of the enterococcus faecalis, the enterococcus faecalis is named as Enterococcus faecalis SX3, and the preservation number of the enterococcus faecalis is CCTCC NO: M 2025248. The enterococcus faecalis has the advantages of being wide in antibacterial spectrum, rapid in growth, high in stress resistance, high in biological safety and the like, and important application value is provided for formulating a scheme for degrading uric acid.

The invention discloses a composition for treating hyperuricemia nephropathy and application thereof, and belongs to the field of biological medicines.The composition is prepared from, by weight, 28-32 parts of rhizoma smilacis glabrae, 28-32 parts of yam rhizome, 13-17 parts of radix clematidis, 8-12 parts of peach kernels, 8-12 parts of flos carthami, 8-12 parts of herba lycopi, 18-22 parts of rhizoma alismatis, 28-32 parts of raw coix seeds, 28-32 parts of semen plantaginis, 13-17 parts of rhizoma atractylodis and 13-17 parts of pseudobulbus cremastrae seu pleiones. The composition can obviously reduce the uric acid level of a mouse with high uric acid nephropathy and improve kidney injury; meanwhile, intestinal flora of mice with the hyperuricemia nephropathy can be remarkably remodeled, abundance of beneficial bacteria Ackerman's bacteria is up-regulated, abundance of opportunistic pathogenic bacteria such as escherichia coli-shigella, desulfurization vibrio and enterococcus is down-regulated, and important significance is achieved for research and drug development of the hyperuricemia nephropathy.

The invention discloses a stichopus japonicus fry cultivation method, and belongs to the technical field of stichopus japonicus fry cultivation. Comprising the following steps: splashing a fermented feed containing a bacillus subtilis fermentation liquid and a bacterial liquid containing an enterococcus faecalis fermentation liquid and a lactobacillus casei fermentation liquid into a culture pond of which the pond bottom is paved with a polyethylene corrugated plate substratum; the fermented feed and the bacterial liquid are continuously splashed until the water quality in the culture pond is clear and transparent, the feed on the corrugated plate is fluffy and is in a short villus shape, and a stable beneficial bacterium phase is formed; peeling off the juvenile sea cucumbers on the 40th day after metamorphosis and attachment, distributing the juvenile sea cucumbers into a cultivation pond for cultivation, and feeding fermented feed and bacterial liquid every day. The method is applied to the aspect of stichopus japonicus fry cultivation, the problems that in existing stichopus japonicus fry production, diseases occur frequently, most fry breeding fields face poor fry quality, and the yield is low are solved, a stable cultivation environment dominated by beneficial bacteria is built, external multi-dimensional disease sources are buffered, blocked and eliminated continuously, and the survival rate of the stichopus japonicus fry is increased. The stichopus japonicus seedlings can grow in the environment without stress.

The invention discloses enterococcus faecalis YL-EF32 and application thereof, and belongs to the technical field of microorganisms. The collection number of the enterococcus faecalis YL-EF32 provided by the invention is CCTCC (China Center For Type Culture Collection) NO: M 20242241. The strain can effectively inhibit the proliferation of chicken-derived clostridium perfringens, has better tolerance to cholate, artificial gastric juice and phenol, and can metabolize dietary fibers such as pectin, glucan, cellobiose and the like. Enterococcus faecalis YL-EF32 is added into daily ration of broiler chickens, necrotic enteritis induced by coccidiosis and clostridium perfringens can be remarkably improved by means of enhancing intestinal barriers, balancing intestinal flora stability, relieving inflammatory response and the like, and the enterococcus faecalis YL-EF32 can be used for resisting clostridium perfringens infection in livestock and poultry breeding.

The invention relates to a composite microbial inoculant and an evaluation method for the antibacterial effect thereof. The method optimizes the in-vitro culture mode of the microbial agent compounded by aerobic bacteria and anaerobic bacteria by simulating the intestinal environment of probiotics in an animal body, and adopts a facultative anaerobic culture method for the microbial compound agent, namely, the outer layer of a sealing film of a triangular flask is wrapped with four layers of preservative films, and the neck of the triangular flask is tightened by a rubber band. The facultative anaerobic environment of aerobic bacillus and facultative anaerobic enterococcus faecalis is considered in the microbial composite inoculant, in the subsequent oxford cup bacteriostasis evaluation experiment, the experiment result with the accurate bacteriostasis effect and good parallelism can be obtained, and the action effect of bacillus and enterococcus faecalis composite bacteria can be efficiently and scientifically evaluated.

The microorganism strain Pediococcus acidilactici TAK 589 CoccobEst is a new antimicrobial and antioxidant probiotic, CoccobEst inhibits the growth of the enteropathogens L. monocytogenes, E. coli, S. enteritidis, S. typhimurium, S. sonnei, S. aureus, E. faecalis and C. sakazakii, but does not inhibit lactobacilli of natural intestinal microbiota. The strain's antimicrobial properties are enhanced by periplasmic peptidoglycan hydrolases with proteolytic activity. CoccobEst is used as a zootechnieai feed additive to prevent bacterial infections in the gastrointestinal tract of farm animals and pets and to prevent and reduce diarrhea. CoccobEst has highly active antioxidant defense system, increasing the redox activity of glutathione and reducing oxidative stress in cells. Therefore, CoccobEst is used as a food supplement or functional food for humans to prevent bacterial infections of the gastrointestinal tract and to prevent and reduce oxidative stress. In addition, CoccobEst is used as a starter culture in fermentation of vegetables.

The invention discloses a method for fermenting vinasse feed through mixed bacteria, and belongs to the field of microbial fermentation. The invention firstly provides a microbial agent for increasing the content of crude protein in fermented vinasse. The microbial agent contains a bacillus subtilis liquid, a corynebacterium glutamicum liquid, a saccharomyces cerevisiae liquid, an enterococcus faecalis liquid and a bifidobacterium animalis liquid. The invention also provides an application of the microbial agent in preparation of a fermented vinasse feed. The contents of crude protein, crude fat and amino acid in the fermented vinasse feed are increased. Through mixed fermentation, the content of amino acid in the vinasse is increased, the content of lysine is increased by 108.33%, and the content of threonine is increased by 59.09%.

An object of the present invention is to provide a new lytic agent capable of lysing Enterococcus bacteria. Provided is a lytic agent for Enterococcus bacteria, consisting of a lytic enzyme or an active fragment thereof, the lytic enzyme comprising: (a) the amino acid sequence shown in SEQ ID NO: 1, (b) an amino acid sequence in which one or multiple amino acids are added, deleted, and / or substituted in the amino acid sequence shown in SEQ ID NO: 1, or (c) an amino acid sequence having a sequence identity of 90% or more with the amino acid sequence shown in SEQ ID NO: 1.

The invention relates to the technical field of microorganisms, in particular to mixed bacteria fermentation liquor as well as a preparation method and application thereof. Bacterial strains in the mixed fermentation liquor comprise clostridium butyricum, enterococcus faecium and bacillus. The preparation method of the mixed bacteria fermentation liquor comprises the following steps: culturing the strains in the mixed bacteria fermentation liquor in a mixed bacteria fermentation culture medium to obtain the mixed bacteria fermentation liquor; wherein the bacterial strains comprise clostridium butyricum, enterococcus faecium and bacillus. According to the mixed bacterium fermentation liquor and the preparation method and application thereof, coexisting culture of donkey source enterococcus faecium, donkey source bacillus and donkey source clostridium butyricum can be achieved through the mixed bacterium fermentation liquor, and meanwhile the mixed bacterium fermentation liquor has the excellent effects of producing compound enzyme and inhibiting pathogenic bacteria and can serve as a feed additive to improve the reproductive performance of a female donkey and improve the growth performance of a donkey foal; the occurrence of donkey foal diarrhea is reduced, so that the economic benefit of donkey breeding is increased.

The present application provides a kind of complex probiotic paste, which combines Enterococcus faecalis, Saccharomyces cerevisiae and vitamin B 12 Producing lactic acid bacteria of canine source, adds prebiotics, flavoring substances and auxiliary substances such as paste matrix preparation.In the paste, the viable count of Enterococcus faecalis, Saccharomyces cerevisiae and lactic acid bacteria is 1-2:1-2:1-8;Paste matrix includes fructooligosaccharide 0.5-3%, psyllium husk powder 0.1-2%, goat milk powder 2-8%, chicken liver powder 1-5%, potassium sorbate 0.5-2%, xanthan gum 1-4%, and the balance is water.The above-mentioned complex probiotic paste can be stably stored for 56 days at 4 DEG C and normal temperature dry place, has good storage stability, good safety, and can adjust intestinal flora and increase the content of intestinal lactic acid bacteria.The above-mentioned complex probiotic paste has good palatability and oral tolerance for pets such as dogs and cats, and has good therapeutic effect on DSS-induced chronic enteritis diarrhea and vitamin B 12 Deficiency.

The invention discloses a walnut probiotic fermented beverage and a preparation method thereof. The beverage is prepared from walnut kernels as a preparation raw material through probiotic fermentation. The walnut kernels are used as preparation raw materials, the beverage is prepared through probiotic fermentation, and the beverage has the advantages of being good in taste, rich in nutrient substance, high in oxidation resistance and the like. The lactobacillus plantarum subsp. Plantarum RF17 and the lactobacillus rhamnosus GOLDGUT-M520 are combined for fermentation, so that the content of total flavonoids in the fermented beverage is increased, and the enterococcus faecalis 2010-JR-01 and the lactobacillus paracasei LCX25 are combined for fermentation, so that the content of total phenols in the fermented beverage is increased.

The present invention relates to a method for treating conditions associated with weight loss in emaciation and cachexia with a probiotic composition and the use of a probiotic composition in the treatment of conditions associated with weight loss in emaciation and cachexia. The present invention provides a method of treating a condition associated with weight loss, the method comprising administering to a subject considered in need of such treatment a probiotic composition wherein the composition comprises at least two bacteria, each preferably selected from a different genus, the genus is preferably selected from the group consisting of Lactobacillus genus, Enterococcus genus and Bifidobacterium genus. The present invention also provides a probiotic composition for use in the treatment of a subject suffering from a condition associated with weight loss wherein the composition comprises at least two bacteria each preferably selected from a different genus, preferably selected from the group consisting of Lactobacillus genus, Enterococcus genus and Bifidobacterium genus.

The invention features E. faecalis phage-derived antimicrobial agent (efagins). The invention also features compositions and methods of using the efagins to treat bacterial infections.

The invention relates to the field of microorganisms, in particular to application of a microbial strain in preparation of a medicine for preventing or treating metabolic diseases, the microbial strain belongs to Enterococcus canini, and the metabolic diseases comprise diabetes, obesity, obesity-related diseases and metabolic syndromes. According to the present invention, with the application of the Enterococcus canintestini, the target spots GPR120 and MC4R for the treatment of obesity diabetes can be significantly activated; the weight, fasting blood glucose, area under a glucose tolerance curve and area under an insulin tolerance curve of obese mice can be reduced, the content of free fatty acid in plasma, total cholesterol in liver and low-density lipoprotein-cholesterol in liver can be reduced, and the content of glucagon-like peptide-1 in plasma and the content of high-density lipoprotein-cholesterol in liver can be increased.

The invention belongs to the technical field of biology, and particularly relates to an antibacterial peptide sourced from symbiotic methane-oxidizing bacteria in deep sea and application of the antibacterial peptide. The amino acid sequence of the antibacterial peptide is shown as SEQ ID NO. 1. The antibacterial peptide provided by the invention is a novel antibacterial peptide, has broad-spectrum antibacterial activity on a variety of gram-negative bacteria and gram-positive bacteria pathogens, such as pseudomonas aeruginosa, listeria monocytogenes, enterococcus faecalis and various vibrios, and has the minimum inhibitory concentration of 8.13-16.25 [mu] M. In addition, the antibacterial peptide has relatively weak hemolytic activity and relatively low cytotoxicity. The antibacterial peptide provided by the invention can replace antibiotics, is applied to prevention and treatment of various bacterial diseases, and is used as a safe and stable food additive or cosmetic preservative candidate substance.

Spray-dried Compositions and Methods of Preparation Spray-dried compositions that may be used in food products, the methods of preparing the compositions and food products containing the compositions are described. The spray-dried composition contains 5 to 50 weight percent (wt. %) of a prebiotic. 10 to 65 wt. % of a probiotic, and 30 to 80 wt. % of a coating material. The prebiotic may be a polysaccharide, an oligosaccharide, a polyol, whey protein, and any combinations thereof. The probiotic may be Lacticaseibacillus, Lactobacillus, Lactiplantibacillus, Levilactobacillus, Ligilactobacillus, Limosilactobacillus, Bifidobacterium, Enterococcus, Streptococcus, Pediococcus, Leuconostoc, Bacillus, Escherichia, Saccharomyces, and any combinations thereof. The coating material may be a synthetic polymer, a natural polymer, and any combinations thereof. The spray-dried compositions are prepared by providing at least one solution containing the composition components and spray drying the at least one solution.

The invention belongs to the field of drug synthesis, relates to an antibacterial drug, and particularly relates to a benzoylaniline derivative and application thereof. A series of benzylamine anilide derivatives are prepared through a total synthesis strategy, and the benzylamine anilide derivatives have the following structural general formula: # imgabs0 #, compared with common commercial antibiotics, the derivative has obviously excellent antibacterial activity, has obviously more excellent antibacterial activity on bacteria including staphylococcus, enterococcus, bacillus, clostridium perfringens and streptococcus, and is expected to become a candidate seedling compound for research and development of novel antibacterial compounds.

The invention discloses a special bioactive soft granular feed for crayfish breeding and a preparation process thereof, comprising the following raw materials in parts by weight: 2 to 8 parts of fish meal, 15 to 25 parts of puffed soybean meal, 5 to 11 parts of rapeseed meal, 6 to 12 parts of wheat bran, 2.5 to 5.5 parts of compound lactic acid bacteria (Lactobacillus plantarum, Lactobacillus acidophilus, Enterococcus faecalis), 3 to 7 parts of shrimp meal, 1 to 3 parts of calcium dihydrogen phosphate, 10 to 30 parts of modified cassava starch, 0.5 to 1.5 parts of fish oil, 0.5 to 1.5 parts of premix, 0.5 to 1.5 parts of vegetable oil, and 15 to 33 parts of fresh maggot paste. The formula of the present invention is more reasonable, nutritionally comprehensive, and each component can be better matched to meet the nutritional needs of crayfish, and can better promote the growth of crayfish, especially in that modified cassava starch and fresh maggot paste are added to the formula, and granulation is carried out at low temperature or room temperature, which can retain the biological activity in the feed to the greatest extent, improve the proportion of animal protein, and be more conducive to crayfish absorption. At the same time, the feed solubility rate is lower, the stability in water is stronger, the feed utilization rate is effectively improved, and the cost of protein raw materials is reduced.

The invention relates to a seawater stichopus japonicus culture pond environment regulation and control method based on a bottom nourishing device and a mixed microbial agent and application. According to the method, the seawater stichopus japonicus culture pond is subjected to bottom nourishing operation, meanwhile, mixed microbial agents of rhodobacter sphaeroides, phosphorus phagocytosis bacillus, moraxella alpha and enterococcus faecalis are splashed, the water quality of the stichopus japonicus culture pond is improved, meanwhile, the immune function of stichopus japonicus is enhanced, and remarkable environmental and economic benefits are achieved.

The invention provides an enterococcus faecalis FS and application thereof. The enterococcus faecalis FS is preserved in the Guangdong Microbial Culture Collection Center on May 12, 2025, and the preservation number of the enterococcus faecalis FS is GDMCC (China General Microbiological Culture Collection Center) 66318. The novel enterococcus faecalis FS is separated from the intestinal tract of healthy adult largemouth bass, has good acid and alkali resistance, cholate resistance, acid production and antibacterial capacity, is good in biological safety and has no toxic or side effect on fishes. When the enterococcus faecalis FS provided by the invention is added into juvenile fish functional feed as a core component, not only can the growth performance and disease resistance of largemouth bass be improved, but also the use of antibiotics can be reduced, the environmental pollution can be reduced, the aquaculture industry can be promoted to develop towards a high-efficiency, environment-friendly and sustainable direction, and the enterococcus faecalis FS has important application value and market prospect.