51 results about "Enterococcus" patented technology

An object of the present invention is to provide a new lytic agent capable of lysing Enterococcus bacteria. Provided is a lytic agent for Enterococcus bacteria, consisting of a lytic enzyme or an active fragment thereof, the lytic enzyme comprising: (a) the amino acid sequence shown in SEQ ID NO: 1, (b) an amino acid sequence in which one or multiple amino acids are added, deleted, and / or substituted in the amino acid sequence shown in SEQ ID NO: 1, or (c) an amino acid sequence having a sequence identity of 90% or more with the amino acid sequence shown in SEQ ID NO: 1.

The invention relates to the technical field of microorganisms, in particular to mixed bacteria fermentation liquor as well as a preparation method and application thereof. Bacterial strains in the mixed fermentation liquor comprise clostridium butyricum, enterococcus faecium and bacillus. The preparation method of the mixed bacteria fermentation liquor comprises the following steps: culturing the strains in the mixed bacteria fermentation liquor in a mixed bacteria fermentation culture medium to obtain the mixed bacteria fermentation liquor; wherein the bacterial strains comprise clostridium butyricum, enterococcus faecium and bacillus. According to the mixed bacterium fermentation liquor and the preparation method and application thereof, coexisting culture of donkey source enterococcus faecium, donkey source bacillus and donkey source clostridium butyricum can be achieved through the mixed bacterium fermentation liquor, and meanwhile the mixed bacterium fermentation liquor has the excellent effects of producing compound enzyme and inhibiting pathogenic bacteria and can serve as a feed additive to improve the reproductive performance of a female donkey and improve the growth performance of a donkey foal; the occurrence of donkey foal diarrhea is reduced, so that the economic benefit of donkey breeding is increased.

The present application provides a kind of complex probiotic paste, which combines Enterococcus faecalis, Saccharomyces cerevisiae and vitamin B 12 Producing lactic acid bacteria of canine source, adds prebiotics, flavoring substances and auxiliary substances such as paste matrix preparation.In the paste, the viable count of Enterococcus faecalis, Saccharomyces cerevisiae and lactic acid bacteria is 1-2:1-2:1-8;Paste matrix includes fructooligosaccharide 0.5-3%, psyllium husk powder 0.1-2%, goat milk powder 2-8%, chicken liver powder 1-5%, potassium sorbate 0.5-2%, xanthan gum 1-4%, and the balance is water.The above-mentioned complex probiotic paste can be stably stored for 56 days at 4 DEG C and normal temperature dry place, has good storage stability, good safety, and can adjust intestinal flora and increase the content of intestinal lactic acid bacteria.The above-mentioned complex probiotic paste has good palatability and oral tolerance for pets such as dogs and cats, and has good therapeutic effect on DSS-induced chronic enteritis diarrhea and vitamin B 12 Deficiency.

The invention relates to a seawater stichopus japonicus culture pond environment regulation and control method based on a bottom nourishing device and a mixed microbial agent and application. According to the method, the seawater stichopus japonicus culture pond is subjected to bottom nourishing operation, meanwhile, mixed microbial agents of rhodobacter sphaeroides, phosphorus phagocytosis bacillus, moraxella alpha and enterococcus faecalis are splashed, the water quality of the stichopus japonicus culture pond is improved, meanwhile, the immune function of stichopus japonicus is enhanced, and remarkable environmental and economic benefits are achieved.

The application discloses a cat oral cavity nursing probiotic composition and application thereof, and the probiotic composition comprises animal bifidobacterium and lactic acid bacteria, wherein the lactic acid bacteria is any one or a combination of multiple of salivarius, lactobacillus casei, enterococcus faecalis, lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus acidophilus and lactobacillus reuteri; compared with single probiotic, the probiotic composition has better effect on oral cavity odor removal, can effectively improve cat oral cavity source halitosis, and can also reduce dental plaque formation, relieve gingivitis and dental caries, and can be applied to preparation of a cat oral cavity odor cleaner, and has good application prospect.

The invention relates to the technical field of biology, in particular to a broad-spectrum bacteriophage lyase mutant, an extraction and purification reagent and a kit. The invention provides a broad-spectrum bacteriophage lyase mutant, an extraction and purification reagent and a kit. A broad-spectrum bacteriophage lyase mutant nucleic acid release liquid containing protein with an amino acid sequence as shown in SEQ ID NO: 1 is adopted; the compound can efficiently act on two important clinical drug-resistant bacteria, namely methicillin-resistant staphylococcus aureus MRSA and vancomycin-resistant enterococcus VRE, at the same time. Through unique molecular design and preparation process optimization, the lyase shows excellent synchronous wall breaking capacity, cell wall destruction can be completed in an extremely short time, and the nucleic acid release efficiency is remarkably superior to that of a traditional method.

The invention discloses a livestock and poultry breeding feed additive capable of reducing cost and improving effect. The livestock and poultry breeding feed additive is prepared from the following raw materials through compound fermentation: probiotic components: bacillus subtilis, clostridium butyricum and enterococcus; the enzyme preparation components comprise cellulose decomposition enzyme and xylanase; a fermentation medium: brown sugar and water; the traditional Chinese medicine auxiliary materials comprise wormwood, health sand and andrographis paniculata. The preparation method of the cost-reducing and effect-improving livestock and poultry breeding feed additive comprises the following steps of bacterium expanding culture, secondary fermentation and product treatment. The invention belongs to the technical field of livestock and poultry breeding, and particularly provides a livestock and poultry breeding feed additive and a preparation method thereof, and the livestock and poultry breeding feed additive can improve the intestinal microecological balance of livestock and poultry, improve the digestion and utilization rate of feed, enhance the immunity of livestock and poultry and reduce the breeding cost through scientific matching of probiotics, enzyme preparations, traditional Chinese medicine components and nutritional auxiliary materials.

The application belongs to the technical field of biotechnology, and provides application of an engineered Enterococcus faecalis capable of expressing human phenylalanine hydroxylase in a medicine for treating phenylketonuria, a plasmid with a human-derived phenylalanine hydroxylase gene after codon optimization of the Enterococcus faecalis is introduced into the Enterococcus faecalis, so that the human phenylalanine hydroxylase is expressed in the intestinal tract. Experimental results show that the human phenylalanine hydroxylase is successfully expressed in the Enterococcus faecalis through genetic engineering technology; the engineered Enterococcus faecalis can significantly metabolize phenylalanine in an in-vitro culture medium system; meanwhile, the engineered Enterococcus faecalis can significantly treat phenylketonuria, and can reduce plasma phenylalanine of a phenylketonuria suckling mouse by 46.8% after 4 weeks of treatment. It is indicated that the engineered Enterococcus faecalis has an outstanding therapeutic effect in preventing and / or treating phenylketonuria.

This invention discloses the application of a morel mycelium extract, belonging to the field of natural products. The morel mycelium extract is mainly composed of α-glucan, with a weight-average molecular weight of 10,000 kDa to 15,000 kDa, a number-average molecular weight of 10,000 kDa to 12,000 kDa, a polydispersity index of 1.0-1.3, and a glucose molar percentage of not less than 99%. The preparation method includes ultrasonic-assisted extraction, alcohol precipitation, enzymatic hydrolysis, dialysis, and ethanol gradient purification. The morel mycelium extract of this invention has the effect of regulating intestinal flora, specifically by improving… Enterococcus , Citrobacter and Bacillus Species abundance of genera, decreased Clostridium and Ruminococcus It has a high species abundance and is more easily utilized by the human gut microbiota than inulin, rapidly decomposing to produce short-chain fatty acids.

This invention provides an in vitro cultured bezoar, its preparation method, and its application, belonging to the field of traditional Chinese medicine preparation technology. The preparation method of this invention involves obtaining a bile-resistant, high-β-glucuronidase-active *Escherichia coli* strain through bile gradient acclimation, which is then combined with *Enterococcus faecalis* to form a functional bacterial community. This community is then cultured in vitro through a three-stage process of proliferation, transformation, and crystallization, followed by gradient calcium ion-induced crystallization and vacuum freeze-drying to obtain in vitro cultured bezoar. This invention aims to solve the problems of low conversion efficiency, uneven crystallization, insufficient content of effective components, and unstable product quality in existing technologies. The obtained in vitro cultured bezoar product has significantly higher bilirubin and conjugated bile acid contents than the standards of the *Chinese Pharmacopoeia*, and the process is stable, controllable, and suitable for industrialization.

The application discloses a dog-derived Enterococcus faecalis ZJUIDS-D016 with anti-aging effect and application, the dog-derived Enterococcus faecalis ZJUIDS-D016 is classified and named as (Enterococcus faecalis) Enterococcus faecalis ), and the preservation number is CGMCC 27038.The strain of the application is isolated from the intestinal tract of a healthy dog, has acid resistance, bile salt resistance and broad-spectrum antibacterial activity, and is sensitive to chloramphenicol, erythromycin and penicillin.The strain of the application has strong host adaptability, can colonize in the intestinal tract of a dog and secrete organic acids and bacteriocins, significantly inhibits the proliferation of common pathogenic bacteria such as dog pathogenic escherichia coli, effectively improves the intestinal health of pets and delays aging, and is suitable for preparing pet probiotic products.

The invention provides a method for preparing ruminant biological feed through fermentation of sodium humate and probiotics, and relates to the field of solid feed fermention.The method comprises the steps that S1, candida utilis powder, lactobacillus plantarum liquid, enterococcus faecium liquid and beet molasses are stirred and mixed, and molasses bacterial liquid is obtained; s2, mixing the molasses bacterial liquid with the treated sodium humate and gelatinized starch urea according to a preparation ratio, and uniformly stirring; and S3, loading into an anaerobic sealed fermentation bag, and naturally fermenting in a greenhouse at the temperature of 25-35 DEG C for 30-48 hours. The biological feed which is rich in high-activity probiotics, high in protein and easy to digest and absorb by animals is prepared from byproducts such as sodium humate, molasses and the like by utilizing biological engineering, and can replace antibiotics in the feed.

The invention belongs to the technical field of feed processing, and particularly provides a preparation method of a bacterium-enzyme synergistic fermentation liquid feed, which comprises the following steps: adding water into feed raw materials, mixing, and adding a compound enzyme preparation for enzymolysis; the compound enzyme preparation comprises alkaline protease and xylanase; cooling the material liquid after enzymolysis, and inoculating a complex microbial inoculant for fermentation; the complex microbial inoculants comprise lactobacillus plantarum, enterococcus faecium and KM71 pichia pastoris; and after the fermentation is finished, sterilizing to obtain the bacterium-enzyme synergistic fermentation liquid feed. According to the preparation method provided by the invention, the pre-enzymolysis of macromolecular substances of the feed and the fermentation effect of microbial flora are cooperated, so that the content of nutrient substances in the feed is remarkably increased. Through sequential addition of an enzyme preparation and a microbial agent, two-stage precise temperature control fermentation and in-situ low-temperature pasteurization technology, fermentation is terminated on the premise of not destroying nutrition, and the problems of nutrition improvement, biogenic amine control and product shelf life are solved.

The invention discloses an enterococcus faecalis bacteriophage with cross-species lysis capability and application of the enterococcus faecalis bacteriophage. The enterococcus faecalis bacteriophage is enterococcus faecalis bacteriophage EF38-P2, and the preservation number of the enterococcus faecalis bacteriophage is CCTCCM20251109; the enterococcus faecalis bacteriophage EF38-P2 is a strong bacteriophage, the enterococcus faecalis bacteriophage EF38-P2 can effectively remove and inhibit proliferation of enterococcus faecalis and enterococcus faecium and can be used for resisting infection of the enterococcus faecalis and the enterococcus faecium, the titer of the enterococcus faecalis bacteriophage EF38-P2 is 3.8 * 10 < 10 > PFU / mL, the enterococcus faecalis bacteriophage EF38-P2 has good activity under the action of a low-concentration chlorine-containing disinfectant, and the enterococcus faecalis bacteriophage EF38-P2 can be used for resisting infection of the enterococcus The enterococcus faecalis bacteriophage EF38-P2 has the advantages that the enterococcus faecalis bacteriophage EF38-P2 is resistant under the condition that the pH value is equal to 3-12, the titer is reduced by not more than 1 order of magnitude within 24 hours, meanwhile, the enterococcus faecalis bacteriophage EF38-P2 has tolerance under the condition that the pH value is equal to 3-12, the titer is reduced by not more than 4 order of magnitude within 96 hours, and the enterococcus faecalis bacteriophage EF38-P2 is good The enterococcus faecalis bacteriophage EF38-P2 can be prepared into medicines for preventing and treating enterococcus faecalis and enterococcus faecium, environment disinfectants, feed additives and the like for application.

A fermented natural substance having nitric oxide metabolites fixated and stabilized therein is produced by a process including adding a fermentative strain to nitrogen-containing natural substance and carrying out fermentation by a batch fermentation process. The nitrogen-containing natural substance is at least one selected from the group consisting of lettuce, Sedum sarmentosum, spinach, blueberry, dandelion, pomegranate, cabbage, garlic, Morinda citrifolia, onion, soybean, bean sprout, mulberry leaf, Momordica charantia, Rubus coreanus, Houttuynia cortada, aronia, Humulus japonicus, Coptis chinensis, hijiki, hooker chive, mushrooms, calamus, wild spinach (Seomcho), Chinese cabbage, kelp, apple, mugwort, orange, salmon testis, abalone shell, shellfish shell, cricket, silkworm, Rehmannia glutinosa, and Shipjeondaebo-tang. to Bacillus sp., Bifidobacterium sp., Enterococcus sp., or Lactobacillus sp.

This invention relates to the field of microbial technology, and more particularly to *Providens skeletalus*, a high-lysine-producing strain, a compound microbial agent, and its applications. This invention isolates *Providens skeletalus* from the intestines of black soldier fly larvae. This strain exhibits high amino acid production, which can improve feed quality and reduce dependence on high-value protein sources. A combination of *Providens skeletalus* with *Enterococcus faecalis* and *Bacillus licheniformis* in a 1:1:1 ratio demonstrates excellent synergistic effects, efficiently degrading organic waste and promoting black soldier fly growth or the synthesis of microbial proteins rich in essential amino acids. This combination has significant application value in the field of organic waste resource utilization.

The invention relates to the technical field of microbial feed additives, and discloses a feed lactic acid bacteria additive and a preparation method thereof.The preparation method comprises the steps that lactobacillus plantarum and / or enterococcus faecalis are / is used as strains for activation and expanding culture, agricultural and sideline product waste is used as a substrate for liquid-state deep fermentation, and mature fermentation liquor is obtained; mixing the mature fermentation liquor with a composite protective agent containing double essential oil, chitosan, theabrownin and porous silicon dioxide, granulating, and pre-drying at low temperature to obtain a semi-dry particle core; and coating with kappa-carrageenan / konjac glucomannan / phytosterol ester composite gel, drying and packaging to obtain a finished product. The double essential oils, the chitosan and the theabrownin are combined to form a full-spectrum active antibacterial system; the elastic composite gel outer layer realizes dual protection of liquid water and water vapor; porous silicon dioxide actively maintains low water activity of particles to prevent lactic acid bacteria from being recovered in advance; the product has the advantages of high viable count, strong heat and acid resistance and excellent storage stability.

The invention relates to the technical field of poultry immunology, in particular to a universal riemerella anatipestifer monoclonal antibody, a detection kit and application of the universal riemerella anatipestifer monoclonal antibody. Specifically, the invention successfully designs the universal riemerella anatipestifer monoclonal antibody, and the detection kit developed based on the monoclonal antibody can rapidly detect the level of the riemerella anatipestifer serum antibody in a sample, and has high sensitivity and strong specificity. The riemerella anatipestifer strain has no cross reactivity with avian enterococcus faecalis, avian salmonella typhimurium, avian escherichia coli and avian salmonella enteritidis, so that the riemerella anatipestifer strain can be applied to rapid screening, epidemiological monitoring and vaccine immune effect evaluation of riemerella anatipestifer infection, and has a wide application prospect.

The invention discloses a single-bacterium fermentation compounding method of cattle feed, and relates to the technical field of feed fermentation. Comprising the following steps: preparing a single-bacterium fermentation bacterial material, the single-bacterium fermentation bacterial material comprises 42.25-47.25 parts of wheat bran, 19-21 parts of soybean meal, 14.25-15.75 parts of corn flour, 19-21 parts of rice hull powder and 0.003-0.005 part of a bacterial liquid, and strains used by the bacterial liquid comprise six probiotics including saccharomyces cerevisiae, bacillus subtilis, bacillus licheniformis, lactobacillus plantarum, enterococcus faecalis and lactobacillus acidophilus; the six probiotics are subjected to independent solid fermentation, the prepared fermentation fungus materials are compounded in proportion to obtain a compound fermentation fungus material, and the compound fermentation fungus material is fed according to cows with different weights. Through the technical route of single-bacterium independent fermentation, the problem of mutual inhibition generated during compound fermentation of various strains is avoided, high-density propagation of the strains in the fermentation process is guaranteed, the compounded feed can meet the nutritional requirements of cattle in different stages, and the yield of the cattle is improved. The feed digestion and absorption rate is effectively improved, the cattle immunity is enhanced, and the growth performance is promoted.

The invention relates to the technical field of feeds, and discloses a functional probiotic-based daily ration formula for laying hens and a preparation method of the functional probiotic-based daily ration formula. Wheat middling is used as daily ration of laying hens, so that the function of reducing the breeding cost is achieved. The laying hen feed is prepared from the following raw materials in parts by weight: corn, soybean meal, 15-30 parts of wheat middling, 0.1-0.2 part of compound probiotic dry powder, 5% laying hen premix and the like according to a scientific proportion, and the compound functional probiotic dry powder is prepared by compounding bacillus licheniformis, bacillus subtilis and enterococcus faecalis. According to the laying hen feed disclosed by the invention, bacillus revives and reproduces in intestinal tracts of laying hens and secretes hydrolases such as xylanase and protease in situ, anti-nutritional factors such as non-starch polysaccharides in wheat middling are efficiently degraded, and wrapped nutrient substances are released; and the lactic acid bacteria regulate the intestinal microecological balance through competitive rejection and maintain the intestinal health. The daily ration can significantly improve the utilization rate of wheat middling, improve the feed-egg ratio, effectively improve the eggshell strength and quality while ensuring the egg laying performance, and assist breeding cost reduction and efficiency improvement.

The invention discloses a primer combination, a kit and a method for detecting sepsis pathogens based on dPCR. The invention discloses a primer composition for detecting sepsis pathogens, which comprises at least one group of primer groups of 28 sepsis pathogens and / or common drug resistance genes such as acinetobacter baumannii, candida, coagulase negative staphylococcus, enterococcus, KPC gene and the like. According to the primer combination for detecting sepsis pathogens based on dPCR, the nucleic acid detection kit containing the primer combination and the detection method, various sepsis pathogens can be rapidly and accurately detected, so that the defect that time and labor are wasted in an existing sepsis pathogen detection technology is overcome, the detection sensitivity and specificity are improved, and the detection cost is reduced. The labor intensity is reduced and the detection period is shortened.

The present invention relates to a composition, pharmaceutical composition, food composition or cosmetic composition, for removing heavy metals, each composition containing one or more lactic acid bacteria selected from the group consisting of bacteria of the genus Lactobacillus, bacteria of the genus Bifidobacterium, bacteria of the genus Enterococcus, bacteria of the genus Pediococcus, and bacteria of the genus Streptococcus. The composition for removing the heavy metals according to the present invention has the advantage of being effectively used to remove heavy metals by expelling them in vivo, due to the composition being excellent for tolerance to heavy metals, antibacterial effect, and heavy metal expulsion.

The present application relates to the technical field of bioengineering, and particularly relates to a recombinant Saccharomyces cerevisiae and a construction method and application thereof, comprising the following steps: step one, using the whole genome of Enterococcus faecalis as a template, a recombinant strain SC1-1 is constructed; step two, using the recombinant strain SC1-1 as a primary strain, L-lactate dehydrogenase CYB2 gene of Saccharomyces cerevisiae is knocked out to obtain a recombinant strain SC2; step three, using the recombinant strain SC2 as a primary strain, branched-chain-2-oxo acid decarboxylase THI3 gene of Saccharomyces cerevisiae is knocked out to obtain a recombinant strain SC3; step four, using the whole genome of Saccharomyces cerevisiae as a template, a recombinant strain SC4 is constructed. The present application innovatively optimizes the source of L-lactate dehydrogenase L-LDH, knocks out L-lactate dehydrogenase CYB2 and branched-chain-2-oxo acid decarboxylase THI3, overexpresses pyruvate kinase CDC19 and H(+)-ATPase PMA2, and makes the L-lactic acid production of Saccharomyces cerevisiae increase to 33.45 g / L.

The invention provides cat-source enterococcus faecalis ZYpet-013 with cholesterol lowering activity and application of the cat-source enterococcus faecalis ZYpet-013. The cat-source enterococcus faecalis ZYpet-013 is derived from intestinal tracts of healthy pet cats, and the preservation number of the cat-source enterococcus faecalis ZYpet-013 is CGMCC No. 32009. The enterococcus faecalis ZYpet-013 is used for preparing products for degrading cholesterol, a cat food spraying composition stably loading the strain and a low-temperature spraying process are developed in a matched mode, the cat food with the cholesterol reducing function is prepared, through daily feeding, the levels of serum total cholesterol and triglyceride of pet cats are safely and effectively reduced, blood fat metabolism is improved, and the cholesterol reducing function is achieved. The requirements of industrial production and shelf life stability are met.

This invention relates to the field of avian immunology, and more particularly to a universal monoclonal antibody against Riemerella anatipestifer, a detection kit, and their applications. Specifically, this invention successfully designed a universal monoclonal antibody against Riemerella anatipestifer, and the detection kit developed based on this monoclonal antibody can rapidly detect the level of Riemerella anatipestifer serum antibodies in samples with high sensitivity and strong specificity. Furthermore, it shows no cross-reactivity with Enterococcus faecalis, Salmonella typhi, Escherichia coli, and Salmonella enteritidis, thus it can be used for rapid screening, epidemiological monitoring, and evaluation of vaccine immunization efficacy against Riemerella anatipestifer infection, with broad application prospects.

The invention discloses a biosynthesis method of alpha-lipoic acid, an engineering strain and a preparation method of the engineering strain. The construction of the engineering strain comprises the following steps: transforming lipocaprylamide enzyme Lpa of enterococcus faecalis, constructing the lipocaprylamide enzyme Lpa to an arabinose promoter or a Cold temperature-sensitive promoter, and then replacing the lipocaprylamide enzyme Lpa to a LacZ gene and an lpxM gene of escherichia coli through RED recombination; or the genes of LipD, lplA, lipA and lpA which are subjected to gene code modification are expressed in the saccharomyces cerevisiae through a pESC vector. The engineering strain is cultured to biologically synthesize alpha-lipoic acid, so that the fermentation cost is further reduced, cyclic synthesis is realized, and the yield is greatly increased.

The present application belongs to the technical field of inactivation of pathogenic microorganisms in water bodies, and discloses a method for inactivating pathogenic microorganisms in water bodies by activating persulfate oxidation with Bordeaux mixture and application thereof. The method is as follows: Bordeaux mixture is added to water bodies contaminated with pathogenic microorganisms, and then a persulfate solution is added to form a reaction system, and the reaction system is shaken at room temperature to simulate real water bodies, so as to inactivate the pathogenic microorganisms. The present application provides a new method for inactivating pathogenic microorganisms such as Escherichia coli and Enterococcus faecalis in water bodies. Sulfate radicals and hydroxyl radicals are generated by activating persulfate with Bordeaux mixture, and alkaline copper sulfate and calcium sulfate precipitate and flocculate and settle the pathogenic bacteria residues in cooperation. The inactivation effect of the Bordeaux / PS system within 5 minutes reaches more than 99%.

The invention relates to molecular glue based on protein in a DUF11 structural domain of enterococcus faecalis. The invention also relates to a nanoparticle vaccine composition formed by the molecular glue, and the immunogen is covalently displayed on the surface of the nanoparticle by the molecular glue.

The invention belongs to the technical field of microbial fermentation traditional Chinese medicine antibiosis, and particularly relates to a probiotic fermented honeysuckle flower water decoction which is obtained by inoculating enterococcus faecalis, bacillus subtilis or bacillus coagulans into the honeysuckle flower water decoction and carrying out fermentation culture. After the probiotic fermented honeysuckle water decoction is fermented, active ingredients of the honeysuckle are fully stimulated, meanwhile, the bacteriostatic effect of the honeysuckle is improved, the effect of an in-vitro bacteriostatic molecular mechanism is affected, and the bacteriostatic effect on pseudomonas aeruginosa is improved.

The objective is to provide a wastewater purification solution for seafood processing plants that purifies wastewater, prevents sludge buildup, and prevents foul odors from being generated from the wastewater. [Solution] The solution is a mixture containing a culture medium obtained by culturing an aqueous solution of molasses with 10 species of bacteria from the genera Aspergillus, Bacillus, Lactopranthibacillus, Enterococcus, Nocardia, Pseudomonas, Ralstonia, Rhizopus, Rhodococcus, and Rhodosprillum, as well as iron fulvic acid, molasses, seaweed powder, and water.