FQ15 enterococcus faecalis and method for producing somatotrophic feed additive with the bacteria

A feed additive and Enterococcus faecalis technology, applied in animal feed, animal feed, bacteria, etc., can solve the problems of poor weight gain effect of micro-ecological feed additives, achieve intestinal flora recovery balance, simple production process, and reduce use Effect

Active Publication Date: 2008-07-16
DALIAN SANYI ANIMAL MEDICINE CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims to solve the technical problem of poor weight gain effect of micro-ecological feed additives in the prior art, and provides a growth-promoting feed additive that can replace feed antibiotics, promote high-efficiency weight gain of livestock and poultry, simple production process, and low cost production method

Method used

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  • FQ15 enterococcus faecalis and method for producing somatotrophic feed additive with the bacteria
  • FQ15 enterococcus faecalis and method for producing somatotrophic feed additive with the bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A FQ15 Enterococcus faecalis CGMCC 2196 belongs to the genus Enterococcus and Enterococcus faecalis species, the strain code is FQ15, and the Latin name is Enterococcus faecalis.

[0036] Isolation and cultivation method of FQ15 Enterococcus faecalis strain:

[0037] After the healthy chickens were killed and dissected, 1 g each of the small intestinal mucosa and intestinal contents or fresh feces were taken by aseptic method, and added to a small bottle filled with 9 mL of sterilized normal saline, mixed evenly and then diluted to a ratio of 10 -4 、10 -5 、10 -6 100 μL of the diluted suspension was added dropwise to three MRS solid medium, spread evenly with a glass rod, and then cultured anaerobically and aerobically at 37°C for 48 h to observe the colony morphology and whether there were calcium-dissolving rings. Select lactic acid bacteria colonies with calcium-dissolving rings and inoculate them on MRS agar slant for pure culture, and store them in a refrigerator ...

Embodiment 2

[0046] The strains used are the same as in Example 1.

[0047] The difference from Example 1 is that the improved MRS medium in the c step is added with 5-30 g of soybean peptone, 1-10 g of glucose, 1-10 g of yeast powder, and 1-10 g of sodium acetate in every liter of MRS medium. Diamine citrate 0.1~8g, Tween80 0.1~5g, dipotassium hydrogen phosphate 0.1~5g, magnesium sulfate 0.05~1g, manganese sulfate 0.001~0.2g, calcium carbonate 1~30g, purified water 1 liter, adjust the pH value to 5.5~ 8.0.

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Abstract

The invention discloses an FQ15 enterococcus faecalis and a method of a feed additive which uses the bacteria production for promoting the growth: an MRS culture medium is taken as a seed culture medium, and the FQ15 enterococcus faecalis is cultured under the aerobic or facultative condition at 30 to 45 DEG C for 4 to 24 hours, so as to become a grade one seed; the grade one seed liquid is inoculated in a seed tank for amplification culture, the MRS culture medium is adopted as the seed culture medium, and the culture is carried out for 4 to 24 hours under the aerobic or the facultative condition at 30 to 45 DEG C to become a grade two seed; the grade two seed liquid is inoculated in a fermentation tank for fermentation culture, an improved MRS culture medium is adopted as the fermentation culture medium, and the culture is carried out for 8 to 36 hours by using timing or continuous fed-batch fermentation mode under the aerobic or the facultative condition at 30 to 45 DEG C; the obtained fermentation liquid is sub-packaged or the fermentation liquid which is obtained by step c is separated, 1 to 25 percent drying protector is added in the bacterial sludge, and the granulation at 20 - 80 DEG C, freeze-drying or spray drying are adopted. The invention has the advantages that the invention can substitute the feed antibiotics and improve the high efficient weight increase of livestock and poultry; the production process is simple; the cost is low, etc.

Description

Technical field: [0001] The invention relates to a feed (feed, drinking water) additive, in particular to a production method of a growth-promoting feed additive which can replace feed antibiotics, promote high-efficiency weight gain of livestock and poultry, simple production process and low cost. Background technique: [0002] With the adjustment of my country's agricultural breeding structure, large-scale and group farming has become a development trend. In order to maximize economic benefits, farmers add feed antibiotics, hormones and probiotic preparations to the feed to promote animal weight gain and purpose of rapid growth. [0003] Adding growth-promoting feed antibiotics and other drugs throughout the breeding period can inhibit and kill the growth and reproduction of intestinal microorganisms in livestock and poultry. On the one hand, it weakens the effective stimulation of intestinal microbes to the epithelial cells of the intestinal wall, inhibits the development...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16
Inventor 庄国宏
Owner DALIAN SANYI ANIMAL MEDICINE CO LTD
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