540 results about "Enterococcus faecalis" patented technology

The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.

The present invention demonstrated the potential use of Lactobacillus johnsonii D115 as a probiotic, as a prophylactic agent or as a surface treatment of materials against human and animal pathogens such as Brachyspira pilosicoli, Brachyspira hyodysenteriae, Shigella sonnei, Vibrio cholera, Vibrio parahaemolyticus, Campylobacter jejuni, Streptococcus pneumoniae, Enterococcus faecalis, Enterococcus faecium, Clostridium perfringens, Yersinia enterocolitica, Escherichia coli, Klebbsiella pneumoniae, Staphylococcus aureus, Salmonella spp., Bacillus cereus, Aspergillus niger and Fusarium chlamydosporum. The proteineous antimicrobial compound was partially characterized and found to be heat tolerant up to 121° C. for 15 min, and acid tolerant up to pH1 for 30 min at 40° C. The compound is also stable to enzymatic digestion, being able to retain more than 60% antimicrobial activity when treated with pepsin and trypsin.

The invention provides a feed fermenting agent, fermented feed and a preparation method thereof, and relates to the technical field of animal product cultivation. The feed fermenting agent is mainly prepared from lactobacillus plantarum, enterococcus faecalis, bacillus subtilis, saccharomyces cerevisiae, lactobacillus cellobiosas, a complex enzyme preparation and the like. The fermented feed is prepared from the feed fermenting agent and fermentation raw materials by using a preparation method of carrying out fermentation in a device which is in one-way exhaust. According to the feed fermenting agent and the fermented feed, disclosed by the invention, the problems of high livestock cultivation cost caused by poor nutritive value, low animal digestion and absorption rate and abuse of antibiotic drugs during cultivation, long fermentation time and slow fermentation starting of existing livestock breeding feed are solved, and high absorption and conversion rate of nutrient contents of the fermented feed are achieved; the fermented feed contains a large amount of probiotics for protecting intestine health of animals, so that the organism immunity of the animals is increased, and the use of antibiotics during livestock cultivation is reduced.

This invention provides isolated nucleic acids encoding polypeptides comprising amino acid sequences of streptococcal matrix adhesion (Ema) polypeptides. The invention provides nucleic acids encoding Group B streptococcal Ema polypeptides EmaA, EmaB, EmaC, EmaD and EmaE. The present invention provides isolated polypeptides comprising amino acid sequences of Group B streptococcal polypeptides EmaA, EmaB, EmaC, EmaD and EmaE, including analogs, variants, mutants, derivatives and fragments thereof. Ema homologous polypeptides from additional bacterial species, including S. pneumoniae, S. pyogenes, E. faecalis and C. diptheriae are also provided. Antibodies to the Ema polypeptides and immunogenic fragments thereof are also provided. The present invention relates to the identification and prevention of infections by virulent forms of streptococci. This invention provides pharmaceutical compositions, immunogenic compositions, vaccines, and diagnostic and therapeutic methods of use of the isolated polypeptides, antibodies thereto, and nucleic acids. Assays for compounds which modulate the polypeptides of the present invention for use in therapy are also provided.

The invention relates to a method for fermentation production of a poultry bacterial agent by multi-bacteria miscible liquid, wherein aerobic bacteria, namely Bacillus subtilis, Bacillus licheniformis, bacillus natto, beer yeast, Aspergillus niger and Aspergillus oryzae are cultured in a shaking table according to different culture mediums, so as to culture a mother seed solution; simultaneously Lactobacillus acidophilus, bifidobacteria and enterococcus faecalis are subjected to anaerobic culture by utilization of Kille flasks, and a mother bacterial solution of photosynthetic bacteria is cultured by a Kille flask under the condition of illumination; the prior stock solution is inoculated into a seed tank for anaerobic culture and fermentation according to 4 percent of the inoculum concentration, and the fermentation time is between 48 and 60 hours; and the cultured seed liquid is inoculated into a productive tank for anaerobic culture and fermentation according to 10 percent of the inoculum concentration, the fermentation time is between 60 and 72 hours, and the fermentation end point is reached when the pH value is reduced to 4.0. The method has the advantages that the microscopic examination viable count of the bacterial agent is more than 5 billion per milliliter, so that the bacterial agent is safe and nontoxic, thereby not only improving the disease resistance of poultry but also promoting the quick growth of the poultry, reducing the feed-meat ratio and improving the quality of meat, eggs and milk.

The invention discloses a kit for quickly detecting 15 pneumonia pathogenic bacteria. The kit can detect streptococcus pneumoniae, staphylococcus aureus, haemophilus influenzae, mycoplasma pneumoniae, pseudomonas aeruginosa, baumanii, enterococcus faecalis, enterococcus faecium, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, stenotrophomonas maltophilia, burkholderia cepacia, legionella pneumophila and chlamydia pneumoniae which cover clinically common pneumonia pathogenic bacteria difficult to culture. 16S rDNA and specific gene sequences corresponding to the pneumonia pathogenic bacteria are detected by combining gene chips with multiple asymmetric PCR reactions, and the categories of the bacteria in a to-be-detected sample are identified in genus and species. The kit makes up for the defect that current clinical detection of pneumonia pathogenic bacteria is not in time or comprehensive and a novel detection means for early diagnosis and early treatment of patients suffering from pneumonia is provided.

The present invention relates to DNA-based methods for universal bacterial detection, for specific detection of the common bacterial pathogens Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, Streptococcus pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Staphylococcus saprophyticus, Streptococcus pyogenes, Haemophilus influenzae and Moraxella catarrhalis as well as for specific detection of commonly encountered and clinically relevant bacterial antibiotic resistance genes directly from clinical specimens or, alternatively, from a bacterial colony. The above bacterial species can account for as much as 80% of bacterial pathogens isolated in routine microbiology laboratories. The core of this invention consists primarily of the DNA sequences from all species-specific genomic DNA fragments selected by hybridization from genomic libraries or, alternatively, selected from data banks as well as any oligonucleotide sequences derived from these sequences which can be used as probes or amplification primers for PCR or any other nucleic acid amplification methods. This invention also includes DNA sequences from the selected clinically relevant antibiotic resistance genes. With these methods, bacteria can be detected (universal primers and/or probes) and identified (species-specific primers and/or probes) directly from the clinical specimens or from an isolated bacterial colony. Bacteria are further evaluated for their putative susceptibility to antibiotics by resistance gene detection (antibiotic resistance gene specific primers and/or probes). Diagnostic kits for the detection of the presence, for the bacterial identification of the above-mentioned bacterial species and for the detection of antibiotic resistance genes are also claimed. These kits for the rapid (one hour or less) and accurate diagnosis of bacterial infections and antibiotic resistance will gradually replace conventional methods currently used in clinical microbiology laboratories for routine diagnosis. They should provide tools to clinicians to help prescribe promptly optimal treatments when necessary. Consequently, these tests should contribute to saving human lives, rationalizing treatment, reducing the development of antibiotic resistance and avoid unnecessary hospitalizations.

The invention discloses an FQ15 enterococcus faecalis and a method of a feed additive which uses the bacteria production for promoting the growth: an MRS culture medium is taken as a seed culture medium, and the FQ15 enterococcus faecalis is cultured under the aerobic or facultative condition at 30 to 45 DEG C for 4 to 24 hours, so as to become a grade one seed; the grade one seed liquid is inoculated in a seed tank for amplification culture, the MRS culture medium is adopted as the seed culture medium, and the culture is carried out for 4 to 24 hours under the aerobic or the facultative condition at 30 to 45 DEG C to become a grade two seed; the grade two seed liquid is inoculated in a fermentation tank for fermentation culture, an improved MRS culture medium is adopted as the fermentation culture medium, and the culture is carried out for 8 to 36 hours by using timing or continuous fed-batch fermentation mode under the aerobic or the facultative condition at 30 to 45 DEG C; the obtained fermentation liquid is sub-packaged or the fermentation liquid which is obtained by step c is separated, 1 to 25 percent drying protector is added in the bacterial sludge, and the granulation at 20 - 80 DEG C, freeze-drying or spray drying are adopted. The invention has the advantages that the invention can substitute the feed antibiotics and improve the high efficient weight increase of livestock and poultry; the production process is simple; the cost is low, etc.

The subject matter of the present invention is a bacteria or mix of bacteria having an antagonistic activity with respect to stains of S. aureus, P. aeruginosa, Streptococcus pyogenes, Enterococcus faecium, Enterobacter cloacae, Proteus mirabilis, Bacteroides fragilis Staphylococcus epidermidis, Propionibacterium acnes, Candida albicans and/or Malassezia furfur as well as the use thereof in the treatment and/or prevention of infections or colonisations related to those pathogens. The invention pertains to care products containing one or more non-pathogenic antagonistic strains intended to prevent and/or treat infections or colonisations on skin, wounds, mucous membranes and appendages.

The present invention discloses a feed micro-ecological preparation, an anti-nutritional substance-free feed and a preparation method thereof, and belongs to the technical field of feed. The feed micro-ecological preparation is prepared by fermenting the following raw materials in parts by weight: 1.0-1.2 parts of Saccharomyces cerevisiae, 0.2-0.6 part of Enterococcus faecalis, 1.5-2.0 parts of Bacillus subtilis, 0.2-0.6 part of Bacillus coagulans, 0.1-0.15 part of Bacillus licheniformis, 0.1-0.3 part of Bacillus lentus, 0.2-0.5 part of Clostridium butyricum and 993.05-995.15 parts of liquid medium. After the feed micro-ecological preparation is mixed with feed raw materials, enzymes produced in the process of microbial strain fermentation can decompose anti-nutritional substances in feed and increase the feed utilization rate; at the same time, microbial strains also have effects in improving animal intestinal environment, maintaining intestinal flora balance, inhibiting the growth of pathogenic bacteria, and improving animal immunity.

The invention discloses enterococcus faecalis HEW-A131 which is high in thermal resistance, wide in acid-alkali resistance range, high in stress resistance and relatively high in probiotic property. The strain is preserved in the China General Microbiological Culture Collection Center on June 17th, 2014, the preservation serial number is CGMCC NO.9353, and the classification name is enterococcus faecalis. The enterococcus faecalis HEW-A131 has excellent microorganism characteristics, remarkable probiotic property and stress resistance and super high fermentation property, not only is the utilization efficiency of feed of animals increased, but also the cost is reduced, moreover the stability of the environment inside the alimentary canals of animals is greatly improved, the adsorption and utilization of nutrition are promoted, the animal growth is promoted, the production performance, immunity and breeding property of the animals are remarkably improved, the production cost is lowered, and the economic benefits are increased.

The invention relates to a novel microorganism feed additive, in particular to a microorganism feed additive which contains bacterial strain that can produce conjugated linoleic acid and a preparation method thereof, which belongs to the microorganism feed additive technical field. The preparation method of the microorganism feed additive is that plant lactobacillus and enterococcus faecalis are respectively processed by zymotic liquid which is cultured by liquid deep-layer high-density fermenting and are made into powder by a micro-capsule peridum spray drying method and a frozen-dried preparation method. The microorganism feed additive transforms linoleic acid into the conjugated linoleic acid in the intestinal tracts of poultry and livestock, which takes effects of promoting the growth performance of the poultry and the livestock, reducing fat, increasing meat factor and regulating immunity reaction, etc., and the microorganism feed additive also solves the problem of the high expense caused by adding the conjugated linoleic acid into the feed directly. The microorganism feed additive prepared by the method has the advantages of high viable bacteria content, long holding period and little microbial pollution.

The invention discloses an animal health care probiotics leavening capable of improving meat quality and application thereof. The leavening includes a component A, a component B and a component C, and a live bacteria proportion in the component A, component B and component C is 1: (0.2-5) : (0.1-5); the component A is a composition of one or two from plant lactobacillus and manure enterococcus faecalis; the component B is composition of one or two from lactobacillus acidophilus and bifidobacterium; and the component C is saccharomyces cerevisiae. The leavening of the invention can be utilizedto prepare probiotics bacteria liquid and fermented feed for animal culture in order to increase meat quality of culture animals. The technical method of the invention is capable of improving meat quality, simple and practical.

The invention provides an antibiotic-free mixed feedstuff and a preparation method thereof. The invention belongs to the field of animal feedstuffs. According to the invention, a mixed feedstuff containing Chinese herbal medicines is mixed with a mixed fermentation material containing various probiotics according to a certain proportion, such that the feedstuff provided by the invention is obtained. The mixed feedstuff containing Chinese herbal medicines comprises half-dried ginkgo leaves or ginkgo leaf extraction residues, Japanese honeysuckle stems, balloon flower root, soybean meal, peanutmeal, corn powder, wheat flour, calcium hydrogen phosphate, common salt, composite microelement, mulvital, and selenium-enriched yeast. The mixed fermentation material containing various probiotics comprises an enterococcus faecalis fermentation material, a bacillus licheniformis fermentation material, a saacharomyces cereisiae fermentation material, a Trichoderma reesei fermentation material, and an aspergillus niger fermentation material, which are mixed according to a certain proportion. Microbe strains are processed through targeted acclimatization, and are cultivated by using a solid fermentation medium, such that the mixed fermentation material is obtained. The antibiotic-free mixed feedstuff provided by the invention assists in promoting growth, and brings no toxic or side-effect. The feedstuff causes no antibiotic residue, no pollution, and no drug resistance. With the mixed fermentation the utilization rate of the feedstuff can be improved, healthy growth of live stocks can be promoted, and meat qualities of the live stocks can be improved.

The invention discloses a preparation method of a tremella fermentation extract and its application. The preparation method of the tremella fermentation extract comprises the following step: tremella undergoes fermentation cultivation by the use of a strain or its culture solution or its suspension so as to obtain a fermentation product, namely the tremella fermentation extract. The strain is at least one of the following strains: zygosaccharomyces rouxii, pasteur yeast, brewer's yeast, wine yeast, acetobacter xylinum, Lactobacillus delbrueckii subsp. Bulgaricus, Streptococcus thermophilus, Bifidobacterium bifidum, lactococcus lactis and enterococcus faecalis. Through experiments, it proves that the tremella fermentation extract prepared by the method contains no chemical components, can be directly used as a finished product of facial mask or essence or toner, is more natural than other existing products in the market and will not have any negative effect on the skin. In addition, smaller components can be obtained by the preparation method in comparison with a common extraction method and the extract is easier to fully absorb by the skin.

The invention discloses a chicken feed probiotic agent containing enterococcus faecalis and a preparation method of the chicken feed probiotic agent. The chicken feed probiotic agent is prepared by virtue of the steps of evenly mixing enterococcus faecalis HEW-A131 active bacterial sludge, bacillus subtilis active bacterial sludge, saccharomyces cerevisiae active bacterial sludge and a stabilization protective agent together in the mass ratio of (0.1-0.8): (1-2.5): (0.1-0.5): 1, and then pelletizing, drying, coating and the like; the probiotic agent is capable of improving the utilization efficiency of an animal feed and saving the cost, and also capable of greatly improving the steady state of the internal environment of the digestive tract in an animal body, promoting the absorption and utilization of nutrition and promoting the growth of the animal; as a result, the productivity, the immune performance and the reproductive performance of the animal are obviously improved, the production cost is reduced and the economic benefit is increased.

The present invention relates to methods for preventing or treating infectious diseases caused by extracellular microorganisms, such as bacteria and fungi, by systemically administering to a patient a compound containing gallium. The extracellular microorganisms targeted by the present methods include methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecalis (VRE), E. coli O157:H7, fluoroquinolone-resistant Salmonella typhi, and the like. Furthermore, in the present methods, gallium compounds can be co-administered with one or more conventional antimicrobial agents to treat infectious diseases with reduced risks of creating multi-drug resistant pathogens. The methods of the present invention is also applicable to those microorganisms, such as ulcer-causing Helicobacter pylori, complete eradication of which so far has been difficult to achieve.

The present invention provides a microbial fermented composite antibiotic-free feed containing natural antibacterial components. The feed comprises a fermented product based on the united segmented fermentation of aerobic-anaerobic composite strains, composite natural antibacterial components, a non-plant source protein/polypeptide compound nutrient supplement agent, and an additive comprising atleast a soybean protein amino acid/polypeptide compound chelate. The composite strains comprises at least lactic acid bacteria, bacillus subtilis, yeast selected from candida utilis or saccharomyces cerevisiae, and enterococcus faecalis. The fermented product based on the united segmented fermentation is prepared by carrying out secondary fermentation on fermentation raw materials through the aerobic-anaerobic composite strains. The combination of the microbial fermented feed and the natural antibacterial components is beneficial to the balance of intestinal flora of animals, and can prevent the dysbacteriosis of digestive tracts, improve the immunity functions of animal organisms, reduce or replace the use of feed antibiotics, and significantly improve the digestion and absorption efficiency of the feed and the meat quality of animals.

The invention discloses an Enterococcus faecalis strain SBD, of which the collection number is CGMCC No.4848. The strain is a Gram positive strain and is spherical according to observation with microscope, and the strain does not form spores; the strain can well grow on a Mann, Rogosa and Sharpe (MRS) agar plate and can grow into a round, smooth and raised bacterial colony like a grey white dew and with a diameter of 0.5 to 1 millimeter within 48 hours; and the strain can grow in a facultatively anaerobic environment, the growth temperature range is from 10 to 50 DEG C, the optimal growth temperature is 30 to 40 DEG C, and the growth pH value range is from 4 to 10 and the optimal pH value is 6.5. The bacterial preparation made by the strain is nontoxic and safe, can resist gastric juice and cholate, has a strong inhibition effect on various harmful bacteria and can be widely used in livestock breeding industry (by directly adding into daily ration or drinking water of animals) to increase the disease resistance in animals; and the preparation is expected to replace antibiotic for feed purpose and can obviously improve average weight of weaned piglets, reduce a feed-to-meat ratio and improve living environment of livestock and therefore has a bright prospect.