41 results about "Enterococcus faecalis" patented technology

The invention relates to the technical field of medicines, and particularly discloses application of a Xinjiang medicine mulberry extract in preparation of a medicine for treating or preventing periapical periodontitis, which is technically characterized in that the Xinjiang medicine mulberry extract is found and proved to have a remarkable inhibition effect on periapical periodontitis key pathogenic bacterium enterococcus faecalis for the first time, the minimum inhibitory concentration (MIC) is 0.604 mg / mL, and the Xinjiang medicine mulberry extract has a remarkable inhibition effect on the periapical periodontitis key pathogenic bacterium enterococcus faecalis. The minimum bactericidal concentration (MBC) is 1.875 mg / mL, and a biological membrane can be effectively inhibited and removed; in-vivo experiments of a rat periapical periodontitis model prove that the extract can remarkably reduce the number of bacteria in root canals and relieve periapical bone destruction, the curative effect is equivalent to that of calcium hydroxide, and the extract is non-toxic to main organs and high in biocompatibility. The invention provides a new candidate and a clear technical scheme for developing an efficient and low-toxicity natural plant source root canal disinfectant.

The present application relates to the technical field of extraction and utilization of chemical substances in walnut green husk, and particularly relates to a production process of urolithin A, in particular to the following steps: obtaining walnut green husk; preparing a fermentation substrate: crushing the walnut green husk into a slurry and adding a stabilizing solution, stirring uniformly and then performing ultrasonic treatment to obtain the fermentation substrate, the stabilizing solution being an aqueous solution of a compound of mercapto-beta-cyclodextrin and chitosan; first fermentation: inoculating microbial liquid A for anaerobic fermentation, then filtering and collecting the filtrate, the microbial liquid A being obtained by inoculating Bacillus velezensis and Bacillus coagulans into culture medium A respectively for activation by shaking culture and then mixing; second fermentation: inoculating microbial liquid B into the filtrate for anaerobic fermentation again, the microbial liquid B being obtained by inoculating Enterococcus faecalis into culture medium B for culture; and extraction, wherein the present application significantly improves the yield and conversion rate of urolithin A by adding the stabilizing solution to the walnut green husk and performing two-stage fermentation, and is safe, environmentally friendly and industrialized.

The invention discloses a glycosyl hydrolase derived from intestinal bacteria and application of the glycosyl hydrolase. A glycosyl hydrolase gene for catalyzing trillioside to be hydrolyzed into diosgenin is cloned from intestinal microorganism enterococcus faecalis for the first time, and protein coded by the glycosyl hydrolase gene is identified as multifunctional glycosyl hydrolase; the diosgenin can be catalyzed to generate diosgenin, and the hydrolysis of other saponins such as steroid saponin and C3, C6 and C21 glycosyl of ginsenoside can be catalyzed, so that a basis is provided for the biological enzymolysis of the diosgenin and other saponins or sapogenin. Meanwhile, biological enzymolysis of the diosgenin makes up for the defects of high production cost, serious environmental pollution and the like of a traditional acid hydrolysis method, and the diosgenin has a great application prospect.

The invention belongs to the field of biological pharmacy, and particularly relates to a chalcanthicidin compound and application thereof. The invention discloses a chalcanthicidin compound which is separated from a secondary metabolite of marine actinomycetes Streptomyces sp. KCB-132 (marine actinomycetes sp. KCB-132). Pharmacological activity experiments show that the compound has a moderate inhibition effect on ESKAPE pathogenic bacteria with multiple drug resistance, such as enterococcus faecium, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumannii and escherichia coli. Therefore, the compound has the potential of being developed into a novel antibacterial agent.

The invention discloses a method for rapidly quantifying live bacteria coated with enterococcus faecium in feed and feed additives. The invention belongs to the technical field of biology, and particularly relates to a rapid quantification method for coating enterococcus faecium viable bacteria in feed and feed additives. The quantitative detection method for the viable bacteria of the coated enterococcus faecium in the feed and the feed additive comprises the following steps: 1) releasing the coated enterococcus faecium in the feed and the feed additive, and separating the viable bacteria of the enterococcus faecium; (2) carrying out molecular identification on the enterococcus faecium viable bacteria obtained in the step (1) by adopting enterococcus faecium specific identification primers; (3) analyzing the viable enterococcus faecium by adopting a quantitative equation to obtain the content of the viable coated enterococcus faecium in the feed and the feed additive.The method successfully solves the technical bottleneck that the viable coated enterococcus faecium is difficult to accurately quantify, shows excellent repeatability, stability and sensitivity, and has a good application prospect.

A kind of fermentation processing method and production equipment system of Gongfu black tea, the Gongfu black tea is formed tea mixture by adding lophatherum gracile, dried tangerine or orange peel and jasmine petals in tea leaves, withering, fermentation, shaping and drying, the fermentation includes pre-fermentation and complete fermentation, wherein pre-fermentation is the self-oxidation fermentation of tea leaves, complete fermentation is microbial fermentation, specifically, first step fermentation is carried out by using acetic acid bacteria and enterococcus faecalis to form compound microbial inoculant, then abnormal hansenula is added to carry out second step fermentation.In the present application, the differences in raw material fermentation process of each component during fermentation are overcome, which leads to the problems of sour and stale taste, etc.in tea leaves.The types and content of aroma substances are increased, and the problem of insufficient infusion resistance of loose tea is solved.The Gongfu black tea prepared by the present application has red and bright golden ring tea soup color, the ratio of teaflavins, theabrownins and thearubigins is coordinated, so that the tea soup is sweet and smooth with honey aroma when entering the mouth, and the aroma is long-lasting in cold cup.

This invention belongs to the field of peptide drugs, specifically relating to an anti-drug-resistant bacterial peptide and its application in pharmaceutical manufacturing. Using Rink amide MBHA amino resin as a solid-phase support, the amino acid sequence of the template peptide raniseptin PL:Ac-GVFDTVKKIGKAVGKFALGVAKNYLNS-NH2 was modified, with amino acid residues 9I and 16F replaced by R8 and S5, respectively, to obtain the target stapling peptide PL-11. Compared to the template peptide PL-0, the stapling peptide PL-11 significantly enhances the antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, and Staphylococcus epidermidis.

This invention belongs to the field of peptide drugs, specifically relating to an anti-drug-resistant bacterial peptide and its application in pharmaceutical manufacturing. Using Rink amide MBHA amino resin as a solid-phase support, the amino acid sequence of the template peptide raniseptin PL:Ac-GVFDTVKKIGKAVGKFALGVAKNYLNS-NH2 was modified, with amino acid residues 6V and 13V replaced by R8 and S5, respectively, to obtain the target stapling peptide PL-10. Compared to the template peptide PL-0, the stapling peptide PL-10 significantly enhances the antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, and Staphylococcus epidermidis.

This invention relates to the field of microbial technology, and more particularly to *Providens skeletalus*, a high-lysine-producing strain, a compound microbial agent, and its applications. This invention isolates *Providens skeletalus* from the intestines of black soldier fly larvae. This strain exhibits high amino acid production, which can improve feed quality and reduce dependence on high-value protein sources. A combination of *Providens skeletalus* with *Enterococcus faecalis* and *Bacillus licheniformis* in a 1:1:1 ratio demonstrates excellent synergistic effects, efficiently degrading organic waste and promoting black soldier fly growth or the synthesis of microbial proteins rich in essential amino acids. This combination has significant application value in the field of organic waste resource utilization.

This invention belongs to the field of peptide drugs, specifically relating to an antibacterial peptide and its application in pharmaceutical manufacturing. Using Rink amide MBHA amino resin as a solid-phase support, the amino acid sequence of the template peptide raniseptin PL:Ac-GVFDTVKKIGKAVGKFALGVAKNYLNS-NH2 was modified, with amino acid residues 13V and 20V replaced by R8 and S5, respectively, to obtain the target stapling peptide PL-13. Compared to the template peptide PL-0, the stapling peptide PL-13 significantly enhances the antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, and Staphylococcus epidermidis.

This invention belongs to the field of livestock and poultry feed technology, specifically disclosing a high-efficiency compound feed for fattening pigs that resists disease and prevents diarrhea, and its preparation method. The feed includes corn, soybean meal, wheat bran, compound functional premix, vegetable oil, dicalcium phosphate, fish meal, salt, citric acid, maifanite, and zeolite. The compound functional premix contains ferrous sulfate, manganese sulfate, copper sulfate, calcium iodate, lysine, Enterococcus faecalis, and Bacillus subtilis. The Enterococcus faecalis and Bacillus subtilis are prepared using a multi-layer coating process. This invention utilizes the above-mentioned high-efficiency compound feed for fattening pigs that resists disease and prevents diarrhea, and its preparation method. This compound feed, through the scientific ratio of compound functional premix and basic feed, combined with innovative multi-layer coating technology and metal loading intercalation technology, achieves efficient colonization of probiotics and synergistic antibacterial effects, significantly improving the intestinal health and production performance of fattening pigs.

The invention belongs to the technical field of oral cavity, and particularly relates to application of probiotics in preparation of a product for accelerating movement of teratodontic teeth. The application innovatively discovers that the probiotic enterococcus faecalis can promote the movement efficiency of orthodontic teeth, is beneficial to shortening the orthodontic time of a patient and improving the orthodontic efficiency, and provides a new research idea for orthodontic treatment. The probiotic enterococcus faecalis is adopted to promote orthodontic dentists to move, infection risks are avoided, operation is convenient, the technical requirement is low, discomfort cannot be caused to patients, the cost is low, and the effect is stable.

The invention discloses a single-bacterium fermentation compounding method of cattle feed, and relates to the technical field of feed fermentation. Comprising the following steps: preparing a single-bacterium fermentation bacterial material, the single-bacterium fermentation bacterial material comprises 42.25-47.25 parts of wheat bran, 19-21 parts of soybean meal, 14.25-15.75 parts of corn flour, 19-21 parts of rice hull powder and 0.003-0.005 part of a bacterial liquid, and strains used by the bacterial liquid comprise six probiotics including saccharomyces cerevisiae, bacillus subtilis, bacillus licheniformis, lactobacillus plantarum, enterococcus faecalis and lactobacillus acidophilus; the six probiotics are subjected to independent solid fermentation, the prepared fermentation fungus materials are compounded in proportion to obtain a compound fermentation fungus material, and the compound fermentation fungus material is fed according to cows with different weights. Through the technical route of single-bacterium independent fermentation, the problem of mutual inhibition generated during compound fermentation of various strains is avoided, high-density propagation of the strains in the fermentation process is guaranteed, the compounded feed can meet the nutritional requirements of cattle in different stages, and the yield of the cattle is improved. The feed digestion and absorption rate is effectively improved, the cattle immunity is enhanced, and the growth performance is promoted.

Provided is a pharmaceutical composition for preventing or treating benign prostatic hyperplasia or alopecia, comprising lactic acid bacteria of Enterococcus genus as an active ingredient. The pharmaceutical composition of the present invention can replace the conventional therapeutic agents against treating benign prostatic hyperplasia or alopecia, such as finasteride or dutasteride. In particular, by introducing heat-killed probiotics of Enterococcus faecalis, the effect of preventing or treating benign prostatic hyperplasia or alopecia can be further enhanced.

The invention relates to the technical field of feeds, and discloses a functional probiotic-based daily ration formula for laying hens and a preparation method of the functional probiotic-based daily ration formula. Wheat middling is used as daily ration of laying hens, so that the function of reducing the breeding cost is achieved. The laying hen feed is prepared from the following raw materials in parts by weight: corn, soybean meal, 15-30 parts of wheat middling, 0.1-0.2 part of compound probiotic dry powder, 5% laying hen premix and the like according to a scientific proportion, and the compound functional probiotic dry powder is prepared by compounding bacillus licheniformis, bacillus subtilis and enterococcus faecalis. According to the laying hen feed disclosed by the invention, bacillus revives and reproduces in intestinal tracts of laying hens and secretes hydrolases such as xylanase and protease in situ, anti-nutritional factors such as non-starch polysaccharides in wheat middling are efficiently degraded, and wrapped nutrient substances are released; and the lactic acid bacteria regulate the intestinal microecological balance through competitive rejection and maintain the intestinal health. The daily ration can significantly improve the utilization rate of wheat middling, improve the feed-egg ratio, effectively improve the eggshell strength and quality while ensuring the egg laying performance, and assist breeding cost reduction and efficiency improvement.

The invention relates to a microbubble drinking type tea yoghourt and a preparation method thereof, and the preparation method comprises the following steps: mixing tea juice, lemon juice, sodium citrate, raw milk and sugar, sterilizing, inoculating with a complex microbial inoculant for fermentation, and demulsifying to obtain the microbubble drinking type tea yoghourt, the strains in the complex microbial inoculant comprise a combination of streptococcus salivarius subsp. Thermophilus, lactococcus lactis subsp. Diacetyl, plant lactobacillus and leuconostoc mesenteroides subsp. Mesenteroides. In the invention, a complex microbial inoculant of four specific microbial strains is adopted to ferment the dairy product, and the four strains are further found to have a synergistic effect, so that the tissue state, the taste, the smell and the overall fusion effect of the tea yoghourt product prepared by final fermentation are further improved; the microbubble tea yoghourt beverage with excellent taste and stable tissue state is prepared.

The invention relates to the field of treatment of obesity through intestinal bacteria transplantation, in particular to a marker combination related to the curative effect of treating obesity through flora transplantation, a product and application of the marker combination and the product. The biomarker composition is prepared from bifidobacterium animalis, ruminococcus sp, bifidobacterium adolescentis, a delazobivibrio sp, a delazobivibrio sp, a Eubacter asiaticus, an enterococcus faecalis and a Citrobacter amalonatus, and the biomarker composition is prepared from the following raw materials: the bifidobacterium animalis, the ruminococcus sp, the bifidobacterium adolescentis, the delazobivibrio sp, the delazobivibrio sp, the Eubacter asiaticus, the Enterococcus faecalis and the Citrobacter amalonatus. The invention provides a reagent for detecting the marker combination, which can be used for preparing a product for predicting / evaluating the curative effect, and also provides a computer readable storage medium containing a storage curative effect judgment instruction, and a curative effect evaluation system containing a detection device and a comparison device. The association between the flora and the obesity curative effect is disclosed for the first time, the method is non-invasive and high in accuracy, the blank that the flora transplantation treatment obesity curative effect lacks a specific marker is filled, and a basis is provided for obesity diagnosis and treatment and individualized treatment.

The invention belongs to the field of medicinal chemistry, and discloses an aromatic pleuromutilin derivative with an antibacterial effect as well as a preparation method and application of the aromatic pleuromutilin derivative. The pleuromutilin is structurally modified, aromatic group modification is further increased through a reductive amination method after mercaptoethylamine is introduced into a C-14 side chain, and the structural formula of the pleuromutilin is shown in the specification. Research on an antibacterial mechanism finds that the series of derivatives can effectively interfere with expression of bacterial protein and show unique and excellent antibacterial activity, and the pleuromutilin can be applied to preparation of antibacterial agents. Compared with pleuromutilin in the prior art, most of the pleuromutilin compounds have antibacterial activity on staphylococcus aureus, escherichia coli, salmonella, micrococcus luteus and enterococcus faecalis which are common in in-vitro clinical application, hardly generate drug resistance, have stable safety and are expected to be further optimized into lead compounds of novel antibiotics.

The invention relates to a flora prediction model for detecting premature delivery risk and a kit and application thereof. Through early-stage mNGS data collection and analysis, premature related strains are obtained, and through a large number of experimental screening and optimization, a set of optimal primer probe group is finally determined. The invention relates to a premature delivery risk calculation method, which comprises the following steps of: selecting 12 floras, namely lactobacillus crispatus, lactobacillus gasseri, lactobacillus inertus, lactobacillus jensenii, escherichia coli, enterococcus faecalis, enterobacter aerogenes, group B hemolytic streptococcus, ureaplasma parvum, chlamydia trachomatis, diplococcus gonorrhoeae and gardnerella vaginalis, and establishing a fluorescent quantitative PCR (Polymerase Chain Reaction) method and a premature delivery risk calculation model aiming at the detection of the 12 floras. Compared with an existing detection method, the method is simple, convenient, rapid, high in sensitivity and high in specificity, the false positive and false negative risk is reduced, and the purpose of batch detection is achieved. Clinically, the change of microbial flora in the reproductive system of a pregnant woman can be rapidly detected, corresponding treatment measures are taken, the risk of PTB occurrence is reduced, and the kit has a good application prospect.

The present invention relates to a novel peptide comprising the amino acid sequence of SEQ ID NO: 1, and an antibacterial composition comprising same. The peptide of the present invention, which is an Enterococcus lactis-derived antibacterial peptide APP101, has specific antibacterial activity against periodontitis-causing bacteria including Porphyromonas gingivalis, Fusobacterium nucleatum, Enterococcus faecalis, Streptococcus parasanguinis, and Streptococcus salivarius, and has stability against proteases, pH, and salt concentrations. The antimicrobial composition of the present invention can be usefully employed for the prevention or treatment of periodontitis.

The present application relates to a kind of abdominal spot frog antibacterial peptide Nv-CATH and its gene and application, the antibacterial peptide Nv-CATH is the circular polypeptide coded by the defense peptide gene of amphibian species unique species abdominal spot frog in China, molecular weight 3356.98 Dalton, isoelectric point 11.56, its amino acid sequence is as shown in SEQ ID NO:1.The gene (GenBank accession No.OP264074) of the precursor of the abdominal spot frog antibacterial peptide Nv-CATH is composed of 714 nucleotide sequences, its nucleotide sequence is as shown in SEQ ID NO:2, wherein the 373-522th nucleotide is the encoding gene of mature abdominal spot frog antibacterial peptide Nv-CATH.The application of abdominal spot frog antibacterial peptide Nv-CATH in preparing the therapeutic drug of the infection disease caused by hemolytic staphylococcus, enterococcus faecalis, staphylococcus aureus, klebsiella pneumoniae, acinetobacter junni and / or paratyphi A.The present application provides a new antibacterial peptide Nv-CATH with strong broad-spectrum antibacterial activity.

This invention belongs to the field of biocontrol microbial technology, specifically relating to a strain of Chaetomium globosum (… Chaetomium globosum )5141-4 、 Inoculants and their applications. This invention isolates an endophytic fungus from *Lithocarpus* lichens and names it *Chaetoceros globosa*. Chaetomium globosum 5141-4. Verification has shown that the above strains can effectively inhibit the growth of Fusarium oxysporum or anthrax, and also exhibit good inhibitory effects against six pathogens, including Pseudomonas aeruginosa and Enterococcus faecalis. They hold promise for use in the preparation of biocontrol products or further screening of antibacterial compounds.

This invention discloses a sedge fermented feed, comprising fermented feed prepared from a fermentation substrate using a fermentation agent and a compound enzyme preparation. The fermentation substrate comprises the following raw materials by weight percentage: 50%-90% sedge, 5%-20% soybean meal, 5%-20% corn, and 2%-5% glucose powder. The fermentation agent includes Bacillus subtilis, Enterococcus faecalis, and yeast. The compound enzyme preparation includes cellulase, xylanase, manganese peroxidase, pectinase, and protease. This invention also discloses a method for preparing sedge fermented feed and its application in preparing monogastric animal feed. By adding a compound enzyme system such as cellulase to sedge and other fermentation substrates, this invention breaks the limitation of sedge being used only in ruminants, effectively reducing feed costs, improving intestinal health, and reducing diarrhea rates.

The invention discloses enterococcus faecium YT1 for degrading lignin and cellulose and application thereof, and relates to the technical field of ensiling, the enterococcus faecium YT1 has a preservation number of CCTCC NO: M 20242768, is preserved in China Center for Type Culture Collection on December 10, 2024, and is preserved in Wuhan University, Wuhan University, No. 299, eight road, Wuchang District, Wuhan City, Hubei Province. The invention also discloses a microbial agent for preparing the silage. The invention also discloses an application of the enterococcus faecium YT1 or the microbial agent in feed silage. The enterococcus faecium YT1 disclosed by the invention can be used for effectively improving the quality of forage silage and enhancing the physiological activity function of silage, and is relatively high in application value.

This invention provides a strain of Bacillus mojavensis, belonging to the field of microbial technology. The Bacillus mojavensis VT275 of this invention, with accession number CGMCC No. 29974, can effectively inhibit common skin pathogens and foodborne pathogens, such as Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Staphylococcus epidermidis, Listeria monocytogenes, Shigella, and Enterococcus faecalis. The Bacillus mojavensis VT275 of this invention can be used to prevent or treat skin diseases such as atopic dermatitis or to control foodborne infections, and has good application prospects in the fields of pharmaceuticals, cosmetics, edible microorganisms, and feed microorganisms.

The invention relates to a probiotic agent for culturing penaeus vannamei boone, and a preparation method and application thereof, the probiotic agent comprises lactobacillus gasseri, and also comprises one or more of lactobacillus plantarum, enterococcus faecalis and saccharomyces cerevisiae cultures; the feed also comprises mannan oligosaccharide; wherein the viable count of lactobacillus gasseri in each gram of the probiotic agent is greater than or equal to 1 * 10 < 9 > CFU; the feed not only can improve the meat taste and delicate flavor of the freshwater cultured penaeus vannamei, increase the mineral content in the muscle of the penaeus vannamei, reduce the content of crude fat, but also can reduce the death rate during freshwater high-density culture.

The invention provides an indole antibacterial peptide simulant with antibacterial activity and a preparation method and application thereof, and relates to the technical field of medicinal chemistry, the chemical structural formula of the indole antibacterial peptide simulant is shown as the following (I), and n and R in the formula are defined as the specification; in-vitro antibacterial activity experiments prove that the indole antibacterial peptide simulant disclosed by the invention has relatively good antibacterial activity on gram-positive bacteria such as staphylococcus aureus, bacillus subtilis and enterococcus faecalis, and has the characteristics of low cytotoxicity, low hemolysis and difficulty in generating drug resistance; the compound can be applied to preparation of drugs for resisting bacteria or treating bacterial infection. (I)

Provided herein are therapeutic agents and compositions for treating infections associated with alcohol-associated hepatitis. Also provided herein are methods of expanding the host range of an Enterococcus faecalis, E. faecalis bacteriophage for the treatment of an alcohol-associated hepatitis associated infection.

This invention discloses an antimicrobial peptide, sesquimycin, and its applications. Specifically, it involves eight sesquimycin-like polypeptide compounds. Compounds 1-8 exhibit varying degrees of antimicrobial activity, with compound 2 possessing broad-spectrum antimicrobial activity. Its minimum inhibitory concentration (MIC) against *Staphylococcus aureus* ATCC 6538 and *Bacillus cereus* CMCC(B)63301 reaches 20 μg / mL, and its MIC against *Enterococcus faecalis* 160119481, *Salmonella typhi* CMCC(B)50071, and *Salmonella typhimurium* CMCC(B)50115 is 40 μg / mL. Given that *Staphylococcus*, *Bacillus*, *Enterococcus*, and *Salmonella* are common foodborne pathogens posing a serious threat to food safety, compounds 1-8 can be used to prepare novel antimicrobial peptides for application in the field of food safety preservation.

The invention discloses a fermented feed for promoting ruminants to efficiently utilize soluble proteins as well as a preparation method and application of the fermented feed, and belongs to the technical field of animal feeds. The fermented feed is prepared by fermenting a substrate consisting of cottonseed meal, corn flour and bran with a compound bacteria solution, and the substrate comprises the following components in percentage by mass: 5-31% of cottonseed meal, 2-31% of corn flour and 38-93% of bran; the mass of the composite bacterial liquid is 8-10% of that of the substrate, and the composite bacterial liquid is prepared by compounding a candida tropicalis bacterial liquid and an enterococcus faecalis bacterial liquid according to a volume ratio of 1: 1, wherein the concentrations of the candida tropicalis bacterial liquid and the enterococcus faecalis bacterial liquid are both 108 CFU / mL. The preparation method comprises the following steps: crushing and sieving the substrate, mixing, adjusting the water content to 40%, spraying the compound bacteria liquid, uniformly mixing, and fermenting at a constant temperature of 30 DEG C. According to the invention, the substrate ratio and the fermentation process are optimized, the utilization efficiency of soluble protein by ruminants is improved, the rumen fermentation environment is improved, meanwhile, the nitrogen emission is reduced, and economic benefits and environmental protection values are achieved in animal production.