125 results about "Probiotic agent" patented technology

The invention relates to a preparation method of a tricholoma matsutake fermentation product with anti-wrinkle and firming effects as well as a product and application of the tricholoma matsutake fermentation product. The preparation method comprises the following steps: mixing a composite plant extract, a carbon source, inorganic salt and water, then sterilizing to obtain a sterilized culture medium, inoculating a composite probiotic agent into the medium, and carrying out fermentation culture to obtain the tricholoma matsutake fermentation product with anti-wrinkle and firming effects. Centrifuging, filtering and sterilizing the culture product to obtain a tricholoma matsutake fermentation product; the bacterial strains in the compound probiotic agent comprise a combination of streptococcus thermophilus, a lactobacillus paracasei YK-L003 bacterial strain and a saccharomyces cerevisiae YK-S001 bacterial strain. According to the invention, specific strains are subjected to compounding and combined fermentation, and the fermentation product filtrate obtained after fermentation treatment of the three strains has an excellent skin conditioning effect, can effectively improve the skin state and resist and weaken wrinkles, and has an excellent effect when being applied to moisturizing and anti-wrinkle cosmetics.

The invention belongs to the technical field of probiotics and application thereof, and relates to a probiotic capable of reducing uric acid and a product thereof, in particular to a strain of Lentilactobacillusbuchneri strain ZD62 which is preserved in China Center for Type Culture Collection on March 14, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M2024469, and the preservation number is CCTCC NO: M2024469, the preservation number is CCTCC NO: M2024469, the preservation number is CCTCC NO: M2024469, and the preservation number is CCTCC NO: M2024469. The invention relates to a compound probiotic agent, which is prepared from Lentilactobacilli buchneri strain ZD62 and Mycobacterium fermentum ZD103, and is characterized in that the Mycobacterium fermentum ZD103 is prepared from Lentilactobacilli buchneri strain ZD62; the lactobacillus buchneri ZD62 and the mucus fermentum ZD103 provided by the invention have a good characteristic of reducing uric acid.

The invention discloses a probiotic fermentation device, and relates to the technical field of fermentation devices, the probiotic fermentation device comprises a fermentation tank, a tank cover is mounted on the fermentation tank, a fixed plate is fixedly connected to the inner wall of the fermentation tank, a gas collection plate is slidably connected to the inner wall of the fermentation tank close to the upper part of the fixed plate, and a ventilation shaft is rotatably connected to the middle part of the tank cover; the middle part of the gas collecting plate is slidably connected to the outer surface of the ventilation shaft, and the outer surface of the middle part of the ventilation shaft is rotatably connected to the fixed plate; compared with the prior art, the device has the advantages that the ventilation shaft rotates under the action of driving force, the ventilation stirring plate can be driven to move together, comprehensive stirring operation is carried out on mixed thalli in the fermentation container, uniform distribution of the thalli is promoted in the stirring process, the contact opportunity of the thalli and oxygen is greatly increased, and the fermentation efficiency is improved. Therefore, the fermentation rate and efficiency of the probiotics are obviously improved.

The invention belongs to the technical field of biological medicine, and discloses a probiotic agent capable of improving the physiological activity function of vitamin D and maintaining bone health and application of the probiotic agent. The invention provides a probiotic agent composed of bifidobacterium adolescentis and bifidobacterium longum, the probiotic agent can significantly improve the bone mineral density, the bone trabecula thickness, the bone trabecula number, the bone trabecula connectivity and the bone volume fraction of an osteoporosis mouse, and meanwhile, the bone trabecula separation degree of the osteoporosis-removed mouse is significantly reduced; the level of vitamin D metabolites such as 25-hydroxy vitamin D and 1, 25-dihydroxy vitamin D in mouse serum is improved; the content of serum calcium and alkaline phosphatase in serum of mice is reduced, and the level of osteocalcin and type I procollagen amino terminal peptide in the serum is improved. The probiotic agent can relieve bone calcium loss, maintain bone health, repair bone injury, increase osteoblasts and reduce the osteoporosis degree, and can be used for preparing drugs for preventing and / or treating osteoporosis or preparing health care products or food beneficial to improving bone mineral density.

The invention provides lactobacillus plantarum XZFMCC101.23155, a probiotic agent and application of the lactobacillus plantarum XZFMCC101.23155 and belongs to the technical field of microorganisms. The invention provides lactobacillus plantarum XZFMCC101.23155, and the preservation number of the lactobacillus plantarum XZFMCC101.23155 is GDMCC No: 65783. The lactobacillus plantarum XZFMCC101.23155 can tolerate hydrogen peroxide, a fermentation product of the lactobacillus plantarum XZFMCC101.23155 has the capacity of removing DPPH, hydroxyl free radicals and superoxide anions, and the total antioxidant activity is high. The fermentation product can also significantly reduce the MDA content in mouse serum, significantly improve the SOD level in mouse serum, improve the GSH content in mouse serum and the like. The lactobacillus plantarum XZFMCC101.23155 and commercial zymophyte are jointly used as a leavening agent to prepare the cheese, and the antioxidant activity of the cheese can be remarkably improved.

The invention discloses a functional probiotic composition and application thereof, and relates to the technical field of microbial engineering. Bifidobacterium longum, lactobacillus acidophilus and enterococcus faecalis are combined to serve as a microbial agent, the microbial agent and active substances of the tea leaves form a composition, then the composition is combined with prebiotics, it is ensured that viable bacteria directly reach the intestinal tract through a microencapsulation technology, and the effect of improving the immune function is achieved; tea polyphenol in the active substances of the tea leaves can serve as a carbon source of prebiotics to stimulate proliferation of the bifidobacterium longum and the lactobacillus acidophilus, so that the acid resistance and intestinal colonization capacity of the microbial inoculum are enhanced, and meanwhile, theanine in the active substances of the tea leaves, the bifidobacterium longum and the lactobacillus acidophilus synergistically promote secretion of gamma-aminobutyric acid, so that the effect of improving the intestinal colonization is achieved. And the tea polyphenol can inhibit the large intestine rod from adhering to the intestinal mucosa, and the probiotic agent secretes antibacterial peptide, so that the integrity of the intestinal barrier is maintained together.

The invention relates to a preparation method of a white truffle fermentation product with an anti-oxidation effect as well as a product and application of the white truffle fermentation product. The preparation method comprises the following steps: mixing a composite plant extract, inorganic salt, water and a composite probiotic agent for fermentation culture, and centrifuging, filtering and sterilizing a culture product to obtain the white truffle fermentation product, the strains in the compound probiotic agent comprise a combination of streptococcus thermophilus, saccharomyces cerevisiae boulardii, a plant lactobacillus YK-L002 strain and a saccharomyces cerevisiae YK-S001 strain. According to the invention, specific strains are subjected to compounding and combined fermentation, and the fermentation product filtrate obtained after fermentation treatment of four bacteria has an excellent skin conditioning effect, can effectively improve the skin state, significantly resist oxidation and resist and weaken wrinkles, and has an excellent effect when being applied to moisturizing and anti-oxidation cosmetics.

The invention relates to the technical field of feed processing, and discloses a lactating sow feed containing fermentation raw materials and a preparation method of the lactating sow feed. A three-layer microcapsule coating auxiliary material is prepared by taking a vitamin premix, an amino acid compound, a compound enzyme preparation and a probiotic agent as core materials through three-layer coating; the basic raw materials comprise corn, wheat, bran, a fermentation raw material, a mineral premix and grease, auxiliary material coating and basic raw material treatment are carried out in parallel in double lines during preparation, and finally, mixing granulation is carried out; the grafted copolymer formed by the amidation reaction of the sodium carboxymethyl cellulose and the chitosan oligosaccharide can cooperate with the sodium alginate to prevent the damage under acidic conditions, and the survival rate of viable bacteria is kept at 85-87% after treatment is carried out for 2 hours under the acidic condition with the pH value of 2.0-3.0; compared with a traditional fermentation raw material coating scheme, the dosage of coating raw materials is reduced by 70% or above; meanwhile, the vitamin premix and the amino acid compound in the auxiliary material can reduce the diarrhea rate by 6% and increase the lactation yield of the sow by 7-18%.

An Akk11 strain-containing probiotic agent capable of relieving hyperuricemia, and the use thereof. Strains in the probiotic agent include an Akkermansia muciniphila Akk11 strain and a Lactobacillus johnsonii LJ09 strain. It is found in the present application that the two strains possess a potential interaction and can cooperate with each other, thereby synergizing the effect of relieving hyperuricemia, which is specifically manifested in: being capable of remarkably regulating uric acid metabolism and improving renal clearance functions; being capable of effectively inhibiting the activity of a key enzyme for uric acid synthesis so as to reduce the production of uric acid, and being capable of remarkably relieving renal damage and renal dysfunctions associated with high levels of uric acid.

Provided are a probiotic formulation for preventing or treating Alzheimer's disease and use thereof. The microorganisms in the probiotic formulation consist of Akkermansia muciniphila and Bifidobacterium breve. Akkermansia muciniphila and Bifidobacterium breve can coordinate with each other after being compounded, so as to provide a synergistic effect on alleviating Alzheimer's disease and thereby a new strategy for preventing or treating Alzheimer's disease. Since Akkermania muciniphila and Bifidobacterium breve are both probiotics, a related functional product prepared from Akkermania muciniphila and Bifidobacterium breve may feature good safety and less likelihood of inducing resistance.

The invention provides application of a composite probiotic agent in cultivation of cynoglossus semilaevis, and belongs to the technical field of aquaculture. The composite probiotic agent provided by the invention is composed of Rhodospira sp., Rhodopseudomonas palustris and Rhodopseudomonas palustris. By mixing the feed for feeding, the intestinal function of the cynoglossus semilaevis can be enhanced, the absorption capacity of the cynoglossus semilaevis on nutrient substances is improved, the immunity of the cynoglossus semilaevis is enhanced, and the disease incidence is reduced; the proportion of harmful bacterium communities in the cynoglossus semilaevis benthic environment can be reduced by spraying the composite probiotic agent to the water bottom mud environment, and the morbidity of the cynoglossus semilaevis is reduced.

The invention provides a probiotic composition for inhibiting helicobacter pylori as well as a probiotic agent and application thereof, and belongs to the technical field of probiotic functional products. The probiotic composition is prepared from the following components in parts by weight: 0.05 to 2 parts of a pediococcus pentosaceus strain A21358, 0.01 to 1.5 parts of a plant lactobacillus strain A21136, 0.05 to 1.5 parts of a lactobacillus rhamnosus strain A21149 and 0.03 to 1.2 parts of a lactobacillus reuteri strain A21325. The probiotic agent comprises the probiotic composition, resistant dextrin, xylooligosaccharide, erythritol, inulin, fructo-oligosaccharide, hericium erinaceus powder, fucoidin and at least one fruit powder selected from grape powder, passion fruit powder and kiwi fruit powder, wherein the specific weight parts of the resistant dextrin, the xylooligosaccharide, the erythritol, the inulin, the fructo-oligosaccharide, the hericium erinaceus powder, the fucoidin and the at least one fruit powder are selected from grape powder, passion fruit powder and kiwi fruit powder. The probiotic agent has the effect of inhibiting helicobacter pylori, and a new thought is provided for clinical related auxiliary treatment of helicobacter pylori infection.

The invention relates to the technical field of microorganisms, in particular to a compound probiotic agent for reducing harmful gases generated in intestinal tracts and regulating intestinal flora. The compound probiotics disclosed by the invention are prepared from bifidobacterium animalis subsp. Lactis Bi66, plant lactobacillus P16 and a saccharomyces boulardii Bld-3 strain. The three strains are compounded and combined for use, so that the content of short-chain fatty acid in intestinal flora can be remarkably increased, the yield of beneficial gases such as CH4 and H2 can be increased, the yield of NH3 and H2S which are harmful to a human body can be reduced, beneficial bacteria can be promoted, and the growth of harmful bacteria can be inhibited, so that the intestinal flora can be regulated. Under the condition of keeping the same amount of the used bacteria, compared with a strain intervention mode without any bacteria, the compounding of the three bacteria has the best effect in the aspect of improving the intestinal flora, which indicates that the three bacteria can be mutually matched and promoted, and can have synergistic interaction in the aspect of regulating the intestinal flora.

The invention provides a method for preparing a biological fermentation heavy metal contaminated soil remediation agent by utilizing ardealite and printing and dyeing sludge, and belongs to the technical field of solid waste recycling. The method comprises the following steps: mixing printing and dyeing sludge, ironmaking solid waste and ardealite, uniformly stirring, granulating, carrying out aging treatment, carrying out high-temperature pyrolysis, and grinding to obtain phosphorus printing carbonized powder; mixing the cordyceps sobolifera mycelium extract, the pandanus elliottii leaf extract and the red velvet extract, and uniformly stirring to obtain an extraction mixture; beta-nicotinamide mononucleotide and galactomannan are mixed and stirred uniformly, and a probiotic agent is obtained; mixing a probiotic agent, the extraction mixture and food powder, and uniformly stirring to obtain enlightening mixed powder; and mixing the heuristic mixed powder with the phosphorus-printed carbonized powder, uniformly stirring, and adding primary rhizosphere compound bacteria for fermentation, thereby obtaining the biological film covering heavy metal contaminated soil remediation agent after fermentation is finished. The efficient utilization of the phosphogypsum and the printing and dyeing sludge is realized through reasonable proportioning, pyrolysis carbonization and primary rhizosphere compound bacteria synergistic fermentation.

The utility model relates to the technical field of traditional Chinese medicine additive processing equipment, in particular to a traditional Chinese medicine additive stirring and mixing device. Comprising a mixing tank, a cover plate and an auxiliary assembly, the auxiliary assembly comprises a stirring motor, a spiral roller, a discharging valve, a temperature sensor, an exhaust component, a feeding component and a heating component, during processing, raw materials are fed into the mixing tank through the feeding component, then the heating component can be used for heating, the temperature sensor is used for detecting internal temperature, the exhaust component can be used for exhausting, and then the stirring motor is started. The stirring motor is controlled to act, the spiral roller can be driven to rotate, automatic mixing is achieved, compared with manual stirring and mixing, the efficiency is higher, the labor intensity of workers can be relieved, finally, materials can be discharged by opening the discharging valve, and therefore automatic stirring and mixing can be achieved, manpower resources are optimized, and the mixing efficiency is improved during probiotic agent processing.

The invention discloses a fertilizer containing herbaceous plant components and a preparation method thereof, and particularly relates to the technical field of agricultural waste, the formula is composed of a composite traditional Chinese medicine extract, a core-shell structure carrier, a functional probiotic agent and an auxiliary agent, and the preparation method comprises the steps of S1, graded extraction of traditional Chinese medicines, S2, carrier loading and microcapsule preparation, and S3, probiotic integration and granulation. Through scientific compatibility of the compound traditional Chinese medicine extracts, design of the core-shell structure carrier, directional addition of the functional probiotics and an optimized preparation process, the prepared fertilizer can synergistically improve the growth index and quality of crops, remarkably enhance the disease and pest resistance, effectively improve the physicochemical properties and micro-ecological environment of soil and improve the yield of the crops. The usage amount of chemical pesticides and fertilizers is reduced, integration of nutrition supply, disease prevention and control and soil improvement is achieved, and the requirements of green agriculture development are met.

The invention discloses a probiotic agent for anaerobic biodegradable plastics, a preparation method of the probiotic agent and biodegradable plastics, the probiotic agent takes PBAT as a carrier material and nano starch, PLA, nano calcium carbonate, carbamide and nano fumed silica as probiotic materials, and probiotic particles are formed through multiple mixing and plasticizing granulation. According to the invention, anaerobic degradation can be regulated and controlled. More importantly, anaerobic degradation also realizes normal use of the degradable plastic, the plastic cannot accelerate degradation in light and oxygen environments, the aging property of the plastic is equivalent to that of a resin matrix of the plastic, and the physical and mechanical properties are ensured.

This invention belongs to the field of food fermentation technology, and specifically relates to a compound probiotic agent and its application in the preparation of fermented soy milk. This invention provides a compound probiotic agent composed of *Lactobacillus fermentum* NCPS LF01, *Pediococcus pentosaceus* NCPS PPE01, and *Lactobacillus acidophilus* NCPS LA01 in a volume ratio of 1-2:1:1, and applies this compound probiotic agent to the preparation of soy products, especially fermented soy milk. The beneficial effects of this invention are that fermented soy milk prepared using this compound probiotic agent has a rich flavor, and the total flavonoid and total phenolic content, as well as the in vitro DPPH free radical scavenging rate, are significantly superior to those of ordinary commercially available strains or other strains. In summary, this compound probiotic agent can effectively improve the retention level of active ingredients and antioxidant capacity of fermented soy milk, and has outstanding application value.

The present invention discloses Lactobacillus kefiranofaciens IMAU12319 and its application, belonging to the technical field of microorganisms, and particularly relates to Lactobacillus kefiranofaciens ( Lactobacillus kefiranofaciens ) IMAU12319, which was deposited on July 8, 2024 at the General Microbiological Center of the China Committee for Culture Collection of Microorganisms, with the deposit address being No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the accession number being: CGMCC No. 31224. The application of Lactobacillus kefiranofaciens IMAU12319 in the preparation of probiotic agents resistant to gastrointestinal digestion. The bacterium of the present invention not only exhibits good gastrointestinal tolerance ability, but also shows strong potential in intestinal adhesion, antioxidant and antibacterial aspects, and has broad application prospects in starter cultures.

The invention relates to the technical field of animal feed, and particularly discloses a bamboo powder dietary fiber probiotic feed additive as well as a preparation method and application thereof. The additive consists of bamboo powder dietary fibers, a probiotic agent and a carrier, wherein probiotics are at least one of trichoderma, bacillus, lactic acid bacteria and saccharomycetes. The preparation method comprises the steps of bamboo powder pretreatment, solid state fermentation, drying, mixing and the like. The product fully utilizes the prebiotics characteristics of the bamboo powder and the regulation effect of probiotics, can effectively improve the intestinal health of livestock and poultry, improve the feed utilization rate and reduce the breeding cost, can partially or completely replace antibiotics, and has the characteristics of greenness, safety and high efficiency.

The invention relates to a fermentation probiotic agent and a preparation method and application thereof.The preparation method of the fermentation probiotic agent comprises the steps that raw materials including, by weight, 2-4 parts of composite probiotic powder, 10-15 parts of fructus psoraleae, 8-13 parts of radix morindae officinalis, 35-42 parts of straw, 5-10 parts of soybean meal, 6-8 parts of trehalose and 18-24 parts of deionized water are weighed; fully mixing the compound probiotic powder, trehalose and deionized water, and performing anaerobic closed culture to obtain activated bacterial liquid for later use; respectively drying the fructus psoraleae, the radix morindae officinalis, the straw and the soybean meal, crushing, sieving with a 100-mesh sieve, uniformly mixing, adding into the activated bacterial liquid, carrying out anaerobic sealed fermentation, and then carrying out vacuum freeze drying to obtain the fermented probiotic agent. The fermented probiotics are used as the feed additive to be added into the basic ration, so that the feed additive has the effects of promoting the growth of livestock animals and improving the feed utilization rate, is remarkable in effect, and also has the effect of improving the immune function of the cattle.

The present application belongs to the technical field of marine probiotics, and discloses an anti-ROS probiotic for coral culture, a screening method of an anti-ROS probiotic agent for coral culture, a use method of the anti-ROS probiotic agent for coral culture, and application of the anti-ROS probiotic agent for coral culture. Based on the great potential of DMSP active microflora in resisting ROS, the present application obtains an anti-ROS probiotic for coral culture, a screening and use method of the probiotic, and application. By using microecological preparations, the activity of probiotics with antioxidant capacity of marine organisms is improved, an antioxidant function system of coral symbiont is constructed and cultured, the peroxide level of coral water is reduced, the immunity and stress resistance potential of coral symbiont are improved, the quality of the environment of the marine aquarium is improved, the risk of coral whitening and disease is effectively reduced, and the treatment and prevention effects on various diseases caused by "oxidative stress" stress response are achieved.

The utility model provides a tobacco probiotic combined rooting water quantitative irrigation device, which belongs to the technical field of tobacco probiotic combined rooting water quantitative irrigation and comprises a solvent adding component and a solute adding component. The solvent adding assembly comprises a hose, a cup body and a water supply switch assembly, the solute adding assembly comprises a probiotic supply pipe and a probiotic flow rate switch, and the hose is arranged on one side of the cup body, is in through connection with the cup body and is used for adding a solvent into the cup body; the water supply switch assembly is arranged on the hose, and the water supply switch assembly is used for temporarily stopping the hose from supplying liquid into the cup body; the utility model provides a tobacco probiotic combined root fixing water quantitative irrigation device which can solve the problems that in the tobacco transplanting process, the adding steps of water and probiotic agents are complex, and the concentration and the liquid amount are different, so that the tobacco growth vigor is different.

The invention relates to an application of a composition of seven probiotics in preparation of an anti-influenza virus probiotic agent. The bacterial strains in the probiotic composition comprise a combination of a streptococcus thermophilus CT-02 bacterial strain, a bifidobacterium animalis subsp. Lactis C-2 bacterial strain, a bifidobacterium longum subsp. Infantis C-3 bacterial strain, a lactobacillus delbrueckii subsp. Bulgaricus CB-01 bacterial strain, a lactobacillus acidophilus CL-01 bacterial strain, a lactobacillus paracasei CL-02 bacterial strain and a lactobacillus rhamnosus CL-03 bacterial strain. New application of the probiotic composition is developed, a research basis is provided for wide application of the probiotic composition, meanwhile, a brand-new strategy for preventing, relieving or treating influenza is also developed, it is found that the probiotic composition has a very remarkable effect in the aspect of relieving related symptoms caused by the influenza, and the probiotic composition has a good application prospect. Specifically, related symptoms such as respiratory system infection, body temperature imbalance, weight loss and vision weakening caused by influenza virus infection are obviously improved.

This invention relates to the field of probiotic technology, specifically to a combination probiotic agent for improving Alzheimer's disease and its application. The combination probiotic agent includes *Lactobacillus gasseri* LFLG-245 and inactivated *Ackermania maughanii* AKKLF; *Lactobacillus gasseri* (… Lactobacillus gasseri LFLG-245 was deposited on March 24, 2025, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 33952. (Ackermania pseudomallei) Akkermansia muciniphila AKKLF was deposited on March 24, 2025, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 33955. The combined bacterial agent provided by this invention can improve Alzheimer's disease.

The invention discloses a probiotic agent for relieving prediabetic action and application thereof, and belongs to the technical field of microorganisms. Experiments prove that bifidobacterium longum CCFM1113 and bifidobacterium adolescentis CCFM1386 composite probiotics can relieve the prediabetic stage, which is specifically embodied in reducing weight gain of mice in the prediabetic stage, regulating blood sugar metabolism, improving sugar tolerance and insulin resistance, protecting liver and pancreas tissues, being beneficial to increasing the diversity of intestinal flora, recovering the structure of the intestinal flora, and improving the immunity of the mice in the prediabetic stage. The invention also provides a preparation method of the bifidobacterium longum CCFM1113 and bifidobacterium adolescentis CCFM1386 composite probiotics. The bifidobacterium longum CCFM1113 and bifidobacterium adolescentis CCFM1386 composite probiotics. And the growth of beneficial bacteria related to metabolic disorder is enriched and regulated. The invention provides an important basis for developing a dietary formula suitable for prediabetic patients, and provides a new method for prevention and management of diabetes mellitus.

The invention discloses probiotic fermented carrot juice capable of improving intestinal health and resisting oxidation and a preparation method of a metagen. The carrot juice is prepared by fermenting carrot raw materials through a compound probiotic agent containing lactobacillus plantarum YZX21, lactobacillus casei YRL577, lactobacillus paracasei X11 and lactobacillus rhamnosus LR-C111. The preparation method comprises the following steps: cleaning, peeling, crushing and pulping carrots to prepare carrot primary pulp, then carrying out high-temperature instantaneous sterilization treatment on the carrot primary pulp, and carrying out vacuum concentration after sterilization; the concentrated carrot primary pulp is inoculated with the composite probiotic agent, the final inoculation concentration is 102-109 CFU / mL, standing fermentation is conducted for 9-15 hours at the temperature of 30-40 DEG C, the probiotic fermented carrot juice is obtained, and the probiotic fermented carrot juice is subjected to high-temperature sterilization treatment to obtain the postbiotics. The probiotic carrot juice and the metabiotics prepared by the invention have the functions of improving constipation, regulating intestinal flora and improving antioxidant capacity, and the preparation method is stable and efficient.

The present application relates to a kind of preparation method of truffle fermentation product with antioxidant effect and its product and application, the preparation method includes the following steps: composite plant extract, inorganic salt, water and composite probiotic agent are mixed to carry out fermentation culture, and the culture product is obtained truffle fermentation product by centrifugation, filtration, sterilization;Strain in the composite probiotic agent includes the combination of streptococcus thermophilus, brady yeast, plant lactobacillus YK-L002 strain and saccharomyces cerevisiae YK-S001 strain.The specific strain of the present application is compounded and combined fermentation, and the fermentation product filtrate after four kinds of bacteria fermentation treatment, with excellent skin conditioning effect, can effectively improve skin state, significantly antioxidant and resist weakening wrinkle, it has excellent effect when being applied in moisturizing antioxidant cosmetic.

The invention relates to a harmless treatment method for solid waste. The compound probiotic microbial agent comprises bacillus licheniformis, lactobacillus rimosii, lactobacillus plantarum, clostridium butyricum and saccharomycetes, the effective viable count is larger than or equal to 0.5 billion / mL, pig manure, corn straw and saw dust are mixed according to the mass ratio to obtain a compost raw material, the initial carbon-nitrogen ratio (C / N) is adjusted to be 25-35, the water content is adjusted to be 55-65%, and the compost raw material is fermented to obtain the compound probiotic microbial agent. And adding a composite probiotic inoculant accounting for 0.5-2.5% of the total mass into the compost raw material. Through probiotic combination and concentration optimization, the problems of decomposition and pollution of various ARGs are solved at the same time.

The application discloses a multi-strain combined solid-state fermentation rapeseed meal probiotic agent and a fermentation method thereof, adopts Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Bacillus subtilis to combine solid-state fermentation rapeseed meal to reduce the content of anti-nutritional factors such as glucosinolates, and comprises the following steps: pretreatment and sterilization of rapeseed meal raw materials, preparation of fermentation seed liquid, solid-state fermentation of probiotics, and preparation of fermented rapeseed meal products. The application firstly uses Bacillus amyloliquefaciens to solid-state ferment rapeseed meal, and combines with Saccharomyces cerevisiae and Bacillus subtilis to add into rapeseed meal to carry out combined fermentation; compared with other technical solutions, the application can greatly reduce the content of anti-nutritional factor glucosinolate in rapeseed meal, and can reduce the content of anti-nutritional factors tannin, phytic acid and crude fiber in rapeseed meal and improve the content of peptides in the rapeseed meal product, various bioactive substances can be produced in the probiotic fermentation process, and the method has the advantages of low cost, simple operation, short time consumption and high efficiency.