88 results about "Probiotic agent" patented technology

The invention belongs to the technical field of biological medicine, and discloses a probiotic agent capable of improving the physiological activity function of vitamin D and maintaining bone health and application of the probiotic agent. The invention provides a probiotic agent composed of bifidobacterium adolescentis and bifidobacterium longum, the probiotic agent can significantly improve the bone mineral density, the bone trabecula thickness, the bone trabecula number, the bone trabecula connectivity and the bone volume fraction of an osteoporosis mouse, and meanwhile, the bone trabecula separation degree of the osteoporosis-removed mouse is significantly reduced; the level of vitamin D metabolites such as 25-hydroxy vitamin D and 1, 25-dihydroxy vitamin D in mouse serum is improved; the content of serum calcium and alkaline phosphatase in serum of mice is reduced, and the level of osteocalcin and type I procollagen amino terminal peptide in the serum is improved. The probiotic agent can relieve bone calcium loss, maintain bone health, repair bone injury, increase osteoblasts and reduce the osteoporosis degree, and can be used for preparing drugs for preventing and / or treating osteoporosis or preparing health care products or food beneficial to improving bone mineral density.

The invention discloses a functional probiotic composition and application thereof, and relates to the technical field of microbial engineering. Bifidobacterium longum, lactobacillus acidophilus and enterococcus faecalis are combined to serve as a microbial agent, the microbial agent and active substances of the tea leaves form a composition, then the composition is combined with prebiotics, it is ensured that viable bacteria directly reach the intestinal tract through a microencapsulation technology, and the effect of improving the immune function is achieved; tea polyphenol in the active substances of the tea leaves can serve as a carbon source of prebiotics to stimulate proliferation of the bifidobacterium longum and the lactobacillus acidophilus, so that the acid resistance and intestinal colonization capacity of the microbial inoculum are enhanced, and meanwhile, theanine in the active substances of the tea leaves, the bifidobacterium longum and the lactobacillus acidophilus synergistically promote secretion of gamma-aminobutyric acid, so that the effect of improving the intestinal colonization is achieved. And the tea polyphenol can inhibit the large intestine rod from adhering to the intestinal mucosa, and the probiotic agent secretes antibacterial peptide, so that the integrity of the intestinal barrier is maintained together.

The invention relates to a preparation method of a white truffle fermentation product with an anti-oxidation effect as well as a product and application of the white truffle fermentation product. The preparation method comprises the following steps: mixing a composite plant extract, inorganic salt, water and a composite probiotic agent for fermentation culture, and centrifuging, filtering and sterilizing a culture product to obtain the white truffle fermentation product, the strains in the compound probiotic agent comprise a combination of streptococcus thermophilus, saccharomyces cerevisiae boulardii, a plant lactobacillus YK-L002 strain and a saccharomyces cerevisiae YK-S001 strain. According to the invention, specific strains are subjected to compounding and combined fermentation, and the fermentation product filtrate obtained after fermentation treatment of four bacteria has an excellent skin conditioning effect, can effectively improve the skin state, significantly resist oxidation and resist and weaken wrinkles, and has an excellent effect when being applied to moisturizing and anti-oxidation cosmetics.

The invention relates to the technical field of feed processing, and discloses a lactating sow feed containing fermentation raw materials and a preparation method of the lactating sow feed. A three-layer microcapsule coating auxiliary material is prepared by taking a vitamin premix, an amino acid compound, a compound enzyme preparation and a probiotic agent as core materials through three-layer coating; the basic raw materials comprise corn, wheat, bran, a fermentation raw material, a mineral premix and grease, auxiliary material coating and basic raw material treatment are carried out in parallel in double lines during preparation, and finally, mixing granulation is carried out; the grafted copolymer formed by the amidation reaction of the sodium carboxymethyl cellulose and the chitosan oligosaccharide can cooperate with the sodium alginate to prevent the damage under acidic conditions, and the survival rate of viable bacteria is kept at 85-87% after treatment is carried out for 2 hours under the acidic condition with the pH value of 2.0-3.0; compared with a traditional fermentation raw material coating scheme, the dosage of coating raw materials is reduced by 70% or above; meanwhile, the vitamin premix and the amino acid compound in the auxiliary material can reduce the diarrhea rate by 6% and increase the lactation yield of the sow by 7-18%.

An Akk11 strain-containing probiotic agent capable of relieving hyperuricemia, and the use thereof. Strains in the probiotic agent include an Akkermansia muciniphila Akk11 strain and a Lactobacillus johnsonii LJ09 strain. It is found in the present application that the two strains possess a potential interaction and can cooperate with each other, thereby synergizing the effect of relieving hyperuricemia, which is specifically manifested in: being capable of remarkably regulating uric acid metabolism and improving renal clearance functions; being capable of effectively inhibiting the activity of a key enzyme for uric acid synthesis so as to reduce the production of uric acid, and being capable of remarkably relieving renal damage and renal dysfunctions associated with high levels of uric acid.

The invention provides a probiotic composition for inhibiting helicobacter pylori as well as a probiotic agent and application thereof, and belongs to the technical field of probiotic functional products. The probiotic composition is prepared from the following components in parts by weight: 0.05 to 2 parts of a pediococcus pentosaceus strain A21358, 0.01 to 1.5 parts of a plant lactobacillus strain A21136, 0.05 to 1.5 parts of a lactobacillus rhamnosus strain A21149 and 0.03 to 1.2 parts of a lactobacillus reuteri strain A21325. The probiotic agent comprises the probiotic composition, resistant dextrin, xylooligosaccharide, erythritol, inulin, fructo-oligosaccharide, hericium erinaceus powder, fucoidin and at least one fruit powder selected from grape powder, passion fruit powder and kiwi fruit powder, wherein the specific weight parts of the resistant dextrin, the xylooligosaccharide, the erythritol, the inulin, the fructo-oligosaccharide, the hericium erinaceus powder, the fucoidin and the at least one fruit powder are selected from grape powder, passion fruit powder and kiwi fruit powder. The probiotic agent has the effect of inhibiting helicobacter pylori, and a new thought is provided for clinical related auxiliary treatment of helicobacter pylori infection.

The invention relates to the technical field of microorganisms, in particular to a compound probiotic agent for reducing harmful gases generated in intestinal tracts and regulating intestinal flora. The compound probiotics disclosed by the invention are prepared from bifidobacterium animalis subsp. Lactis Bi66, plant lactobacillus P16 and a saccharomyces boulardii Bld-3 strain. The three strains are compounded and combined for use, so that the content of short-chain fatty acid in intestinal flora can be remarkably increased, the yield of beneficial gases such as CH4 and H2 can be increased, the yield of NH3 and H2S which are harmful to a human body can be reduced, beneficial bacteria can be promoted, and the growth of harmful bacteria can be inhibited, so that the intestinal flora can be regulated. Under the condition of keeping the same amount of the used bacteria, compared with a strain intervention mode without any bacteria, the compounding of the three bacteria has the best effect in the aspect of improving the intestinal flora, which indicates that the three bacteria can be mutually matched and promoted, and can have synergistic interaction in the aspect of regulating the intestinal flora.

The invention discloses a fertilizer containing herbaceous plant components and a preparation method thereof, and particularly relates to the technical field of agricultural waste, the formula is composed of a composite traditional Chinese medicine extract, a core-shell structure carrier, a functional probiotic agent and an auxiliary agent, and the preparation method comprises the steps of S1, graded extraction of traditional Chinese medicines, S2, carrier loading and microcapsule preparation, and S3, probiotic integration and granulation. Through scientific compatibility of the compound traditional Chinese medicine extracts, design of the core-shell structure carrier, directional addition of the functional probiotics and an optimized preparation process, the prepared fertilizer can synergistically improve the growth index and quality of crops, remarkably enhance the disease and pest resistance, effectively improve the physicochemical properties and micro-ecological environment of soil and improve the yield of the crops. The usage amount of chemical pesticides and fertilizers is reduced, integration of nutrition supply, disease prevention and control and soil improvement is achieved, and the requirements of green agriculture development are met.

This invention belongs to the field of food fermentation technology, and specifically relates to a compound probiotic agent and its application in the preparation of fermented soy milk. This invention provides a compound probiotic agent composed of *Lactobacillus fermentum* NCPS LF01, *Pediococcus pentosaceus* NCPS PPE01, and *Lactobacillus acidophilus* NCPS LA01 in a volume ratio of 1-2:1:1, and applies this compound probiotic agent to the preparation of soy products, especially fermented soy milk. The beneficial effects of this invention are that fermented soy milk prepared using this compound probiotic agent has a rich flavor, and the total flavonoid and total phenolic content, as well as the in vitro DPPH free radical scavenging rate, are significantly superior to those of ordinary commercially available strains or other strains. In summary, this compound probiotic agent can effectively improve the retention level of active ingredients and antioxidant capacity of fermented soy milk, and has outstanding application value.

The invention relates to the technical field of animal feed, and particularly discloses a bamboo powder dietary fiber probiotic feed additive as well as a preparation method and application thereof. The additive consists of bamboo powder dietary fibers, a probiotic agent and a carrier, wherein probiotics are at least one of trichoderma, bacillus, lactic acid bacteria and saccharomycetes. The preparation method comprises the steps of bamboo powder pretreatment, solid state fermentation, drying, mixing and the like. The product fully utilizes the prebiotics characteristics of the bamboo powder and the regulation effect of probiotics, can effectively improve the intestinal health of livestock and poultry, improve the feed utilization rate and reduce the breeding cost, can partially or completely replace antibiotics, and has the characteristics of greenness, safety and high efficiency.

The present application belongs to the technical field of marine probiotics, and discloses an anti-ROS probiotic for coral culture, a screening method of an anti-ROS probiotic agent for coral culture, a use method of the anti-ROS probiotic agent for coral culture, and application of the anti-ROS probiotic agent for coral culture. Based on the great potential of DMSP active microflora in resisting ROS, the present application obtains an anti-ROS probiotic for coral culture, a screening and use method of the probiotic, and application. By using microecological preparations, the activity of probiotics with antioxidant capacity of marine organisms is improved, an antioxidant function system of coral symbiont is constructed and cultured, the peroxide level of coral water is reduced, the immunity and stress resistance potential of coral symbiont are improved, the quality of the environment of the marine aquarium is improved, the risk of coral whitening and disease is effectively reduced, and the treatment and prevention effects on various diseases caused by "oxidative stress" stress response are achieved.

The invention relates to an application of a composition of seven probiotics in preparation of an anti-influenza virus probiotic agent. The bacterial strains in the probiotic composition comprise a combination of a streptococcus thermophilus CT-02 bacterial strain, a bifidobacterium animalis subsp. Lactis C-2 bacterial strain, a bifidobacterium longum subsp. Infantis C-3 bacterial strain, a lactobacillus delbrueckii subsp. Bulgaricus CB-01 bacterial strain, a lactobacillus acidophilus CL-01 bacterial strain, a lactobacillus paracasei CL-02 bacterial strain and a lactobacillus rhamnosus CL-03 bacterial strain. New application of the probiotic composition is developed, a research basis is provided for wide application of the probiotic composition, meanwhile, a brand-new strategy for preventing, relieving or treating influenza is also developed, it is found that the probiotic composition has a very remarkable effect in the aspect of relieving related symptoms caused by the influenza, and the probiotic composition has a good application prospect. Specifically, related symptoms such as respiratory system infection, body temperature imbalance, weight loss and vision weakening caused by influenza virus infection are obviously improved.

This invention relates to the field of probiotic technology, specifically to a combination probiotic agent for improving Alzheimer's disease and its application. The combination probiotic agent includes *Lactobacillus gasseri* LFLG-245 and inactivated *Ackermania maughanii* AKKLF; *Lactobacillus gasseri* (… Lactobacillus gasseri LFLG-245 was deposited on March 24, 2025, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 33952. (Ackermania pseudomallei) Akkermansia muciniphila AKKLF was deposited on March 24, 2025, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 33955. The combined bacterial agent provided by this invention can improve Alzheimer's disease.

The invention discloses a probiotic agent for relieving prediabetic action and application thereof, and belongs to the technical field of microorganisms. Experiments prove that bifidobacterium longum CCFM1113 and bifidobacterium adolescentis CCFM1386 composite probiotics can relieve the prediabetic stage, which is specifically embodied in reducing weight gain of mice in the prediabetic stage, regulating blood sugar metabolism, improving sugar tolerance and insulin resistance, protecting liver and pancreas tissues, being beneficial to increasing the diversity of intestinal flora, recovering the structure of the intestinal flora, and improving the immunity of the mice in the prediabetic stage. The invention also provides a preparation method of the bifidobacterium longum CCFM1113 and bifidobacterium adolescentis CCFM1386 composite probiotics. The bifidobacterium longum CCFM1113 and bifidobacterium adolescentis CCFM1386 composite probiotics. And the growth of beneficial bacteria related to metabolic disorder is enriched and regulated. The invention provides an important basis for developing a dietary formula suitable for prediabetic patients, and provides a new method for prevention and management of diabetes mellitus.

The invention discloses probiotic fermented carrot juice capable of improving intestinal health and resisting oxidation and a preparation method of a metagen. The carrot juice is prepared by fermenting carrot raw materials through a compound probiotic agent containing lactobacillus plantarum YZX21, lactobacillus casei YRL577, lactobacillus paracasei X11 and lactobacillus rhamnosus LR-C111. The preparation method comprises the following steps: cleaning, peeling, crushing and pulping carrots to prepare carrot primary pulp, then carrying out high-temperature instantaneous sterilization treatment on the carrot primary pulp, and carrying out vacuum concentration after sterilization; the concentrated carrot primary pulp is inoculated with the composite probiotic agent, the final inoculation concentration is 102-109 CFU / mL, standing fermentation is conducted for 9-15 hours at the temperature of 30-40 DEG C, the probiotic fermented carrot juice is obtained, and the probiotic fermented carrot juice is subjected to high-temperature sterilization treatment to obtain the postbiotics. The probiotic carrot juice and the metabiotics prepared by the invention have the functions of improving constipation, regulating intestinal flora and improving antioxidant capacity, and the preparation method is stable and efficient.

The present application relates to a kind of preparation method of truffle fermentation product with antioxidant effect and its product and application, the preparation method includes the following steps: composite plant extract, inorganic salt, water and composite probiotic agent are mixed to carry out fermentation culture, and the culture product is obtained truffle fermentation product by centrifugation, filtration, sterilization;Strain in the composite probiotic agent includes the combination of streptococcus thermophilus, brady yeast, plant lactobacillus YK-L002 strain and saccharomyces cerevisiae YK-S001 strain.The specific strain of the present application is compounded and combined fermentation, and the fermentation product filtrate after four kinds of bacteria fermentation treatment, with excellent skin conditioning effect, can effectively improve skin state, significantly antioxidant and resist weakening wrinkle, it has excellent effect when being applied in moisturizing antioxidant cosmetic.

The invention relates to a harmless treatment method for solid waste. The compound probiotic microbial agent comprises bacillus licheniformis, lactobacillus rimosii, lactobacillus plantarum, clostridium butyricum and saccharomycetes, the effective viable count is larger than or equal to 0.5 billion / mL, pig manure, corn straw and saw dust are mixed according to the mass ratio to obtain a compost raw material, the initial carbon-nitrogen ratio (C / N) is adjusted to be 25-35, the water content is adjusted to be 55-65%, and the compost raw material is fermented to obtain the compound probiotic microbial agent. And adding a composite probiotic inoculant accounting for 0.5-2.5% of the total mass into the compost raw material. Through probiotic combination and concentration optimization, the problems of decomposition and pollution of various ARGs are solved at the same time.

The application discloses a multi-strain combined solid-state fermentation rapeseed meal probiotic agent and a fermentation method thereof, adopts Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Bacillus subtilis to combine solid-state fermentation rapeseed meal to reduce the content of anti-nutritional factors such as glucosinolates, and comprises the following steps: pretreatment and sterilization of rapeseed meal raw materials, preparation of fermentation seed liquid, solid-state fermentation of probiotics, and preparation of fermented rapeseed meal products. The application firstly uses Bacillus amyloliquefaciens to solid-state ferment rapeseed meal, and combines with Saccharomyces cerevisiae and Bacillus subtilis to add into rapeseed meal to carry out combined fermentation; compared with other technical solutions, the application can greatly reduce the content of anti-nutritional factor glucosinolate in rapeseed meal, and can reduce the content of anti-nutritional factors tannin, phytic acid and crude fiber in rapeseed meal and improve the content of peptides in the rapeseed meal product, various bioactive substances can be produced in the probiotic fermentation process, and the method has the advantages of low cost, simple operation, short time consumption and high efficiency.

The present invention relates to a preparation method and product and application of a matsutake fermented product with anti-wrinkle and firming effects, the preparation method comprising: sterilizing a composite plant extract, a carbon source, an inorganic salt, and water after mixing to obtain a sterilized culture matrix, accessing a composite probiotic agent into the matrix for fermentation and culture, and obtaining a matsutake fermented product by centrifugation, filtration, and sterilization of the culture product; the strains in the composite probiotic agent include a combination of thermophilic Streptococcus, Lactobacillus paracasei YK-L003 strain, and Saccharomyces cerevisiae YK-S001 strain. The present invention combines specific strains for composite and combined fermentation, and the fermented product filtrate after fermentation by the three bacteria has excellent skin conditioning effect, can effectively improve skin condition, resist weakening wrinkles, and has excellent effect in moisturizing and anti-wrinkle cosmetics.

The invention discloses a strain of Ligilbacillus murinus PV211184 capable of improving the reproductive performance of livestock, which belongs to the field of agricultural animal husbandry, is preserved in Guangdong Province Microbial Culture Collection Center, has a preservation number of GDMCC No: 66039, has a bacterial colony in milk white forms such as round bulge, smoothness and the like, has a suitable growth temperature of 30-45 DEG C, a pH value of 4.0-6.5, preferably 37 DEG C and a pH value of 6.0, and is subjected to facultative anaerobic culture by using an MRS culture medium. The bacterium can be colonized in the uterus, has good endometrial epithelial cell adhesion capability, has no negative influence on sperm motility and IVF embryonic development, and can promote endometrial epithelial cell proliferation. Experiments show that the litter size per litter of a sow can be increased by 1.3, the litter size per litter can be increased by 1.0, the lambing rate of Hu sheep can be increased by 7.2%, the lambing rate per litter can be increased by 14.5%, and the feed can be used as a probiotic agent for improving the reproductive performance of livestock.

The invention discloses pediococcus pentosaceus TRM54998 with broad-spectrum antibacterial activity and application of the pediococcus pentosaceus TRM54998, and belongs to the field of microorganisms. The strain is preserved in the China Center for Type Culture Collection (CCTCC) on June 04, 2024, and the preservation number is CCTCC NO: M 20241142. It is proved that the strain has good acid resistance and bile salt resistance and can grow in the environment with the bovine bile salt concentration being 0.1-0.2% (w / v) or in the pH value range being 3-7; the fermentation supernatant has an inhibition effect on nine pathogenic bacteria such as salmonella and the like, and the antibacterial effect is stable; meanwhile, experiments prove that the pediococcus pentosaceus TRM54998 has no wide drug resistance, can relieve the problems of aggravated drug resistance, damage to microbial ecological balance and the like caused by antibiotic abuse, provides a strain resource for preparing a biological bacteriostatic agent or an intestinal probiotic agent, and has a wide application prospect.

The invention discloses bifidobacterium longum subsp. Infantis i18 with an effect of regulating intestinal flora and application of the bifidobacterium longum subsp. Infantis i18, and belongs to the technical field of microbial engineering. The bifidobacterium longum subsp. Infantis i18 strain is preserved in the China General Microbiological Culture Collection Center, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 25, 2024, the preservation number is CGMCC NO.31411, and the Latin name is Bifidobacterium longum subsp. Infantis. The invention also discloses a preparation method of the bifidobacterium longum subsp. Infantis strain. The bifidobacterium longum subsp. Infantis i18 provided by the invention has a bidirectional regulation effect, can relax bowel, relieve diarrhea, maintain intestinal steady state and regulate intestinal flora at the same time, expands the application range of the bifidobacterium longum subsp. Infantis, and enriches the types of the domestic bifidobacterium longum subsp. Infantis. The preparation method is suitable for preparing the probiotic agent or the infant formula milk powder.

The invention belongs to the technical field of plant planting, and provides a Hainan camellia oleifera high-survival-rate drought-resistant afforestation and biological nutrient solution preparation method. The special nutrient solution for the camellia oleifera comprises the following raw materials in parts by weight: 6-12 parts of a probiotic agent, 8-12 parts of potassium fulvate, 15-20 parts of potassium nitrate, 60-70 parts of a coco coir extract and 95-105 parts of water. Through the synergistic effect of the afforestation method and the special nutrient solution for camellia oleifera, the survival rate and preservation rate of camellia oleifera afforestation are remarkably increased. Wherein the special nutrient solution has high-temperature-resistant, drought-resistant and root-promoting effects, and can effectively promote the growth of roots of camellia oleifera, improve the water-retaining capacity of leaves and increase the content of available phosphorus in soil, thereby enhancing the stress resistance and growth vigor of plants; in the drought period, the leaf wilting degree of the oil-tea camellia adopting the drought-resistant afforestation method is far lower than that of a traditional method, the water content of soil is higher, and stage drought stress can be effectively resisted.

This invention relates to a process for recycling and treating distiller's grains, comprising: pretreatment: solid-liquid separation of distiller's grains to obtain solid material and microbial-containing liquid, adjusting the carbon-to-nitrogen ratio of the solid material to 18-30:1; enzymatic hydrolysis: adding a compound enzyme preparation and an enzyme stabilizer to the solid material for enzymatic hydrolysis to obtain enzymatic hydrolysis products; microbial fermentation: adding a compound probiotic agent to the enzymatic hydrolysis products for fermentation to form fermented material, with microbial-containing liquid introduced during fermentation; grading: grading the fermented material through a vibrating screen to obtain coarse and fine particles; high-temperature treatment: subjecting the coarse particles to high-temperature steam explosion treatment; surface activation: surface modification treatment of the fine particles; secondary fermentation: mixing the high-temperature treated coarse particles with the fine particles, and adding the compound probiotic agent again for secondary fermentation to obtain fermented products; and drying and shaping. This recycling process has high processing efficiency, a shorter fermentation cycle, and higher nutritional content.

The present application relates to tea tree planting technical field, especially effective control tea cake disease occurrence's tea garden annual management method, the present application provides a kind of effective control tea cake disease occurrence's tea garden annual management method, respectively in March, May, July spray compound probiotic agent;In November, base fertilizer is applied.The tea garden annual management method provided by the present application has obvious growth promotion effect on tea tree, compared with other control groups, the color of tender leaf is more tender green, bud is more neat, and the length of tender leaf and the number of bud are obviously improved.The method also has obvious prevention and treatment effect on tea cake disease, and the prevention and treatment effect is obviously better than that of the existing technology.

The utility model provides a probiotic agent fermentation device and relates to the field of probiotics. The probiotic fermentation device comprises a fermentation shell and a center control plate, the outer circumferential surface of the fermentation shell is fixedly connected with a positioning ring, the top of the positioning ring is fixedly connected with a supporting base, the inner wall of the supporting base is slidably connected with a limiting insertion pin, and the supporting base is rotatably connected with a reinforcing plate; the top of the supporting base is fixedly connected with a component protection shell, the inner wall of the component protection shell is slidably connected with a guide position control cylinder, and one side of the guide position control cylinder is fixedly connected with a force application jacking column. According to the probiotic agent fermentation device, through the arrangement of the fermentation shell, the central control plate, the positioning ring, the supporting base, the limiting insertion pin, a reinforcing plate, a component protection shell, a guide position control cylinder, a force application jacking column and a tough spring, a scraping plate can be quickly taken out, so that yeast adhered to the surface of the scraping plate is cleaned, the cleanliness of the scraping plate is ensured, and the scraping plate can be conveniently used next time.

This invention relates to the field of microbial technology, and particularly to a compound probiotic agent for reducing the production of harmful intestinal gases and regulating intestinal flora. The compound probiotic of this invention consists of *Bifidobacterium animalis* subsp. *lactobacter* Bi66, *Lactobacillus plantarum* P16, and *Saccharomyces boulardii* strain Bld-3. This invention combines these three strains to significantly increase the content of short-chain fatty acids in the intestinal flora, increase the production of beneficial gases such as CH4 and H2, and reduce the production of harmful gases such as NH3 and H2S, thereby promoting the growth of beneficial bacteria and inhibiting the growth of harmful bacteria and regulating the intestinal flora. While maintaining a consistent amount of bacteria, compared with intervention methods lacking any one of the strains, the combination of the three strains showed the best effect in improving the intestinal flora, indicating that the three strains can cooperate and promote each other, and can synergistically enhance the effect in regulating the intestinal flora.

The invention relates to the field of bacterial strains, and discloses a composite probiotic agent and application thereof in improving the flavor of corn puree. According to the composite probiotic agent, sodium alginate and chitosan are subjected to functional group modification to form an embedding material with enhanced hydrophobicity and water solubility, and a pH-sensitive double-layer microcapsule structure is constructed by synergistically embedding plant lactobacillus, lactobacillus mucus fermentation and saccharomyces cerevisiae. The probiotic agent is applied to fermentation of the corn puree, probiotics can be effectively protected to pass through a gastric acid environment, targeted release of intestinal tracts is achieved, meanwhile, the corn puree is promoted to generate rich flavor substances through multi-bacterium synergistic fermentation, and the sweet feeling, milk fragrance and the overall flavor level of a product are remarkably improved. The process conditions are mild, large-scale production is easy, and the obtained corn puree beverage is rich and mellow in flavor and has the probiotic function.

The invention discloses application of a bifidobacterium longum subsp. Infantis CCFM1426 strain and vitamin A in preparation of a medicine for improving the physiological activity of the vitamin A and / or relieving colitis and / or xerophthalmia symptoms. The probiotic agent provided by the invention comprises a bifidobacterium longum subsp. Infantis CCFM1426 strain with the preservation number of GDMCC No.65152 and vitamin A, and can be used for improving the retinoic acid level in the colon of a mouse with ulcerative colitis; the diamine oxidase level, the intestinal fatty acid binding protein level and the lipopolysaccharide binding level in serum of mice with ulcerative colitis are reduced; the corneal injury, basal vacuolation and conjunctival goblet cell injury of the xerophthalmia mice are relieved; the level of retinoic acid in serum of the dry-eye mouse is improved; and the levels of IL-6, IL-17 and TNF-alpha in serum of dry eye mice are reduced. Therefore, the probiotic agent disclosed by the invention has a relatively great application prospect in preparation of products for supplementing vitamin A and preventing and / or treating ulcerative colitis and xerophthalmia.

The invention relates to novel paenibacillus with biomacromolecule degradation capacity and nitrogen fixation and phosphorus solubilization effects and application of the novel paenibacillus, the strain is identified as a novel strain of paenibacillus through multi-phase classification, and the novel paenibacillus has the capacity of degrading biomacromolecules such as starch, cellulose, casein, chitin and lecithin. The method can be applied to degradation of related carbohydrates, proteins and lipid substances and preparation of biological enzymes; the bacillus subtilis has the advantages that the bacillus subtilis can be used for preparing the bacillus subtilis, has the phosphorus dissolving capacity of dissolving organic phosphorus and inorganic phosphorus, has nitrogenase and beta-1, 3-glucanase and the activity of generating IAA (Indoleacetic Acid), can play a probiotic role on crops by fixing nitrogen, dissolving phosphorus and inhibiting pathogenic bacteria, can grow in an environment with the salinity not higher than 4.5%, and has certain salt-tolerant activity. Due to multiple functions, the strain can be used for preparing and applying biological nitrogen fertilizers, phosphate fertilizers and crop probiotics, so that the strain plays an important role in crop growth and green agricultural production.