150 results about "Probiotic agent" patented technology

The invention discloses lactobacillus kefiranofaciens IMAU12319 and application thereof, belongs to the technical field of microorganisms, and particularly relates to lactobacillus kefiranofaciens IMAU12319 which is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 8, 2024, the preservation address is No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, and the number is CGMCC No.31224. The lactobacillus kefiranofaciens IMAU12319 has the advantages that the lactobacillus kefiranofaciens IMAU12319 can be used for preparing the lactobacillus kefiranofaciens IMAU12319, and the lactobacillus kefiranofaciens IMAU12319 can be used for preparing the lactobacillus kefiranofaciens IMAU12319; the invention also relates to an application of the lactobacillus kefiranofaciens IMAU12319 in preparation of a probiotic agent capable of resisting gastrointestinal digestion. The strain disclosed by the invention not only shows good gastrointestinal tract endurance capacity, but also shows relatively strong potential in the aspects of intestinal adhesion, oxidation resistance and bacteriostasis, and has a wide application prospect in leavening agent strains.

The invention relates to a preparation method of a tricholoma matsutake fermentation product with anti-wrinkle and firming effects as well as a product and application of the tricholoma matsutake fermentation product. The preparation method comprises the following steps: mixing a composite plant extract, a carbon source, inorganic salt and water, then sterilizing to obtain a sterilized culture medium, inoculating a composite probiotic agent into the medium, and carrying out fermentation culture to obtain the tricholoma matsutake fermentation product with anti-wrinkle and firming effects. Centrifuging, filtering and sterilizing the culture product to obtain a tricholoma matsutake fermentation product; the bacterial strains in the compound probiotic agent comprise a combination of streptococcus thermophilus, a lactobacillus paracasei YK-L003 bacterial strain and a saccharomyces cerevisiae YK-S001 bacterial strain. According to the invention, specific strains are subjected to compounding and combined fermentation, and the fermentation product filtrate obtained after fermentation treatment of the three strains has an excellent skin conditioning effect, can effectively improve the skin state and resist and weaken wrinkles, and has an excellent effect when being applied to moisturizing and anti-wrinkle cosmetics.

The invention belongs to the technical field of probiotics and application thereof, and relates to a probiotic capable of reducing uric acid and a product thereof, in particular to a strain of Lentilactobacillusbuchneri strain ZD62 which is preserved in China Center for Type Culture Collection on March 14, 2024, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M2024469, and the preservation number is CCTCC NO: M2024469, the preservation number is CCTCC NO: M2024469, the preservation number is CCTCC NO: M2024469, and the preservation number is CCTCC NO: M2024469. The invention relates to a compound probiotic agent, which is prepared from Lentilactobacilli buchneri strain ZD62 and Mycobacterium fermentum ZD103, and is characterized in that the Mycobacterium fermentum ZD103 is prepared from Lentilactobacilli buchneri strain ZD62; the lactobacillus buchneri ZD62 and the mucus fermentum ZD103 provided by the invention have a good characteristic of reducing uric acid.

The invention discloses a probiotic fermentation device, and relates to the technical field of fermentation devices, the probiotic fermentation device comprises a fermentation tank, a tank cover is mounted on the fermentation tank, a fixed plate is fixedly connected to the inner wall of the fermentation tank, a gas collection plate is slidably connected to the inner wall of the fermentation tank close to the upper part of the fixed plate, and a ventilation shaft is rotatably connected to the middle part of the tank cover; the middle part of the gas collecting plate is slidably connected to the outer surface of the ventilation shaft, and the outer surface of the middle part of the ventilation shaft is rotatably connected to the fixed plate; compared with the prior art, the device has the advantages that the ventilation shaft rotates under the action of driving force, the ventilation stirring plate can be driven to move together, comprehensive stirring operation is carried out on mixed thalli in the fermentation container, uniform distribution of the thalli is promoted in the stirring process, the contact opportunity of the thalli and oxygen is greatly increased, and the fermentation efficiency is improved. Therefore, the fermentation rate and efficiency of the probiotics are obviously improved.

The invention belongs to the technical field of biological medicine, and discloses a probiotic agent capable of improving the physiological activity function of vitamin D and maintaining bone health and application of the probiotic agent. The invention provides a probiotic agent composed of bifidobacterium adolescentis and bifidobacterium longum, the probiotic agent can significantly improve the bone mineral density, the bone trabecula thickness, the bone trabecula number, the bone trabecula connectivity and the bone volume fraction of an osteoporosis mouse, and meanwhile, the bone trabecula separation degree of the osteoporosis-removed mouse is significantly reduced; the level of vitamin D metabolites such as 25-hydroxy vitamin D and 1, 25-dihydroxy vitamin D in mouse serum is improved; the content of serum calcium and alkaline phosphatase in serum of mice is reduced, and the level of osteocalcin and type I procollagen amino terminal peptide in the serum is improved. The probiotic agent can relieve bone calcium loss, maintain bone health, repair bone injury, increase osteoblasts and reduce the osteoporosis degree, and can be used for preparing drugs for preventing and / or treating osteoporosis or preparing health care products or food beneficial to improving bone mineral density.

The invention provides lactobacillus plantarum XZFMCC101.23155, a probiotic agent and application of the lactobacillus plantarum XZFMCC101.23155 and belongs to the technical field of microorganisms. The invention provides lactobacillus plantarum XZFMCC101.23155, and the preservation number of the lactobacillus plantarum XZFMCC101.23155 is GDMCC No: 65783. The lactobacillus plantarum XZFMCC101.23155 can tolerate hydrogen peroxide, a fermentation product of the lactobacillus plantarum XZFMCC101.23155 has the capacity of removing DPPH, hydroxyl free radicals and superoxide anions, and the total antioxidant activity is high. The fermentation product can also significantly reduce the MDA content in mouse serum, significantly improve the SOD level in mouse serum, improve the GSH content in mouse serum and the like. The lactobacillus plantarum XZFMCC101.23155 and commercial zymophyte are jointly used as a leavening agent to prepare the cheese, and the antioxidant activity of the cheese can be remarkably improved.

According to the probiotic agent for enhancing the immunity and improving the intestinal flora and the application of the probiotic agent, five specific different types of bifidobacterium strains are compounded, potential interaction exists among the five strains, and the five strains can cooperate with one another, so that the effect of enhancing the immunity and improving the intestinal flora is achieved. The bifidobacteria has the advantages that the bifidobacteria has synergistic interaction in the aspects of promoting the growth of immune organs and increasing the total quantity and diversity of intestinal flora, the bifidobacteria is matched with basic fermentation strains for yogurt fermentation, meanwhile, the composite prebiotics are added for synergistic yogurt fermentation, the quantity of the bifidobacteria at the end of a shelf life after the yogurt fermentation is finished is still not less than 1 * 10 < 8 > CFU / mL or 1 * 10 < 8 > CFU / g, and the content of the bifidobacteria is not less than the stability of lactic acid bacteria in the yoghourt is remarkably improved, so that the number of viable bacteria which enter the intestinal tract of a human body and play roles is remarkably increased, and the yoghourt has relatively good tolerance in the intestinal tract; the prepared yoghurt is fine and smooth in texture, rich in taste and rich in fragrance; in addition, the immune decline and intestinal flora disorder caused by cyclophosphamide (CTX) can be remarkably improved, and a positive effect on immunological enhancement is achieved.

The invention relates to a probiotic agent for regulating an intestinal environment and an application of the probiotic agent. The bacterial strains in the probiotic agent for regulating the intestinal environment comprise a Bifidobacterium animalis subsp. Lactis C-1 bacterial strain and a Lactobacillus gasseri CKCC 1913 bacterial strain, and the bacterial strains in the probiotic agent for regulating the intestinal environment comprise a lactobacillus plantarum subsp. Lactis C-1 bacterial strain and a lactobacillus gasseri CKCC 1913 bacterial strain, a lactobacillus plantarum subsp. Lactis CKCC 1913 bacterial strain, a lactobacillus plantarum subsp. Lactis CKCC 1913 bacterial strain and a lactobacillus plantarum subsp. Lactis CKCC 1913 bacterial strain. A synergistic interaction relationship exists between the two bacteria, so that the intestinal micro-ecological environment can be regulated, the intestinal injury can be relieved and the intestinal barrier function can be improved by reducing the level of inflammatory factors, balancing the content of gastrointestinal regulatory peptides, promoting intestinal peristalsis and other action mechanisms. Therefore, the probiotic agent is suitable for preparing a preparation with the efficacy of preventing, improving or treating constipation and other inflammatory diseases.

The invention discloses a functional probiotic composition and application thereof, and relates to the technical field of microbial engineering. Bifidobacterium longum, lactobacillus acidophilus and enterococcus faecalis are combined to serve as a microbial agent, the microbial agent and active substances of the tea leaves form a composition, then the composition is combined with prebiotics, it is ensured that viable bacteria directly reach the intestinal tract through a microencapsulation technology, and the effect of improving the immune function is achieved; tea polyphenol in the active substances of the tea leaves can serve as a carbon source of prebiotics to stimulate proliferation of the bifidobacterium longum and the lactobacillus acidophilus, so that the acid resistance and intestinal colonization capacity of the microbial inoculum are enhanced, and meanwhile, theanine in the active substances of the tea leaves, the bifidobacterium longum and the lactobacillus acidophilus synergistically promote secretion of gamma-aminobutyric acid, so that the effect of improving the intestinal colonization is achieved. And the tea polyphenol can inhibit the large intestine rod from adhering to the intestinal mucosa, and the probiotic agent secretes antibacterial peptide, so that the integrity of the intestinal barrier is maintained together.

The invention provides a lactobacillus plantarum KU01, and the preservation number of the lactobacillus plantarum KU01 is CGMCC (China General Microbiological Culture Collection Center) No.31788. The lactobacillus plantarum KU01 has the advantages that the preservation number is CGMCC No.31788; the invention also provides a fermentation product of the plant lactobacillus and a probiotic preparation comprising the plant lactobacillus and the fermentation product thereof. According to the probiotic agent and the preparation method thereof, the anti-helicobacter pylori probiotic agent is obtained through peracid stress fermentation, peracid stress fermentation, bacterial liquid separation, embedding, compounding and sieving. According to the method, on the basis of the inherent anti-helicobacter pylori physiological activity of the probiotics, the anti-helicobacter pylori physiological activity of the probiotics and the fermentation product is enhanced through stress fermentation, the fermentation product and the probiotics are sequentially released through an embedding technology, and an exogenous iron source is matched; and the anti-helicobacter pylori physiological activity is further promoted. The probiotics provided by the invention can be applied to resisting helicobacter pylori.

The invention relates to a preparation method of a white truffle fermentation product with an anti-oxidation effect as well as a product and application of the white truffle fermentation product. The preparation method comprises the following steps: mixing a composite plant extract, inorganic salt, water and a composite probiotic agent for fermentation culture, and centrifuging, filtering and sterilizing a culture product to obtain the white truffle fermentation product, the strains in the compound probiotic agent comprise a combination of streptococcus thermophilus, saccharomyces cerevisiae boulardii, a plant lactobacillus YK-L002 strain and a saccharomyces cerevisiae YK-S001 strain. According to the invention, specific strains are subjected to compounding and combined fermentation, and the fermentation product filtrate obtained after fermentation treatment of four bacteria has an excellent skin conditioning effect, can effectively improve the skin state, significantly resist oxidation and resist and weaken wrinkles, and has an excellent effect when being applied to moisturizing and anti-oxidation cosmetics.

The invention relates to the technical field of feed processing, and discloses a lactating sow feed containing fermentation raw materials and a preparation method of the lactating sow feed. A three-layer microcapsule coating auxiliary material is prepared by taking a vitamin premix, an amino acid compound, a compound enzyme preparation and a probiotic agent as core materials through three-layer coating; the basic raw materials comprise corn, wheat, bran, a fermentation raw material, a mineral premix and grease, auxiliary material coating and basic raw material treatment are carried out in parallel in double lines during preparation, and finally, mixing granulation is carried out; the grafted copolymer formed by the amidation reaction of the sodium carboxymethyl cellulose and the chitosan oligosaccharide can cooperate with the sodium alginate to prevent the damage under acidic conditions, and the survival rate of viable bacteria is kept at 85-87% after treatment is carried out for 2 hours under the acidic condition with the pH value of 2.0-3.0; compared with a traditional fermentation raw material coating scheme, the dosage of coating raw materials is reduced by 70% or above; meanwhile, the vitamin premix and the amino acid compound in the auxiliary material can reduce the diarrhea rate by 6% and increase the lactation yield of the sow by 7-18%.

The invention provides a lactobacillus rhamnosus strain A22004 with anti-inflammatory and anti-oxidation functions as well as a derivative product and application of the lactobacillus rhamnosus strain A22004, and belongs to the technical field of functional microorganisms. A lactobacillus rhamnosus strain A22004 is separated and purified from feces of Guangxi 100-year old people. The lactobacillus rhamnosus strain A22004 has good antioxidant and anti-inflammatory activity, and can be used for preparing probiotics, health care products or related drugs for maintaining intestinal health.

An Akk11 strain-containing probiotic agent capable of relieving hyperuricemia, and the use thereof. Strains in the probiotic agent include an Akkermansia muciniphila Akk11 strain and a Lactobacillus johnsonii LJ09 strain. It is found in the present application that the two strains possess a potential interaction and can cooperate with each other, thereby synergizing the effect of relieving hyperuricemia, which is specifically manifested in: being capable of remarkably regulating uric acid metabolism and improving renal clearance functions; being capable of effectively inhibiting the activity of a key enzyme for uric acid synthesis so as to reduce the production of uric acid, and being capable of remarkably relieving renal damage and renal dysfunctions associated with high levels of uric acid.

The invention relates to the technical field of aquaculture, and discloses a preparation method of an aquatic fermented feed containing a composite probiotic inoculant, and the preparation method comprises the following steps: mixing bacillus amyloliquefaciens, streptococcus lactis and saccharomycetes in proportion to obtain the composite probiotic inoculant; the preparation method comprises the following steps: dissolving a compound probiotic agent and a fermentation aid in water, spraying the dissolved compound probiotic agent and the fermentation aid into the raw materials, adding a probiotic agent for mixing, quantitatively sub-packaging the mixed raw materials into breathing bags, and performing solid-state fermentation at the fermentation temperature of 25-40 DEG C for 3-7 days to obtain the aquatic product fermented feed. The method has the effect of improving the feed utilization rate.

The invention provides a lactic acid bacteria composition for adjuvant treatment of diabetes, a compound lactic acid bacteria probiotic agent and application, and belongs to the technical field of functional microorganisms. The lactic acid bacteria composition provided by the invention is obtained by compounding eight specific lactic acid bacteria strains, has the effects of reducing blood sugar, blood fat and insulin level for people with high blood sugar and diabetics, and also has the effect of regulating gastrointestinal tract disorder. Therefore, the compound lactic acid bacteria probiotic agent prepared on the basis of the lactic acid bacteria composition has a good blood sugar control effect on people with high blood sugar and patients with type II diabetes mellitus. Meanwhile, the compound lactic acid bacteria probiotic agent has no side effect, and the purpose of safe prevention and treatment can be achieved.

Provided are a probiotic formulation for preventing or treating Alzheimer's disease and use thereof. The microorganisms in the probiotic formulation consist of Akkermansia muciniphila and Bifidobacterium breve. Akkermansia muciniphila and Bifidobacterium breve can coordinate with each other after being compounded, so as to provide a synergistic effect on alleviating Alzheimer's disease and thereby a new strategy for preventing or treating Alzheimer's disease. Since Akkermania muciniphila and Bifidobacterium breve are both probiotics, a related functional product prepared from Akkermania muciniphila and Bifidobacterium breve may feature good safety and less likelihood of inducing resistance.

The invention provides application of a composite probiotic agent in cultivation of cynoglossus semilaevis, and belongs to the technical field of aquaculture. The composite probiotic agent provided by the invention is composed of Rhodospira sp., Rhodopseudomonas palustris and Rhodopseudomonas palustris. By mixing the feed for feeding, the intestinal function of the cynoglossus semilaevis can be enhanced, the absorption capacity of the cynoglossus semilaevis on nutrient substances is improved, the immunity of the cynoglossus semilaevis is enhanced, and the disease incidence is reduced; the proportion of harmful bacterium communities in the cynoglossus semilaevis benthic environment can be reduced by spraying the composite probiotic agent to the water bottom mud environment, and the morbidity of the cynoglossus semilaevis is reduced.

The invention provides a probiotic composition for inhibiting helicobacter pylori as well as a probiotic agent and application thereof, and belongs to the technical field of probiotic functional products. The probiotic composition is prepared from the following components in parts by weight: 0.05 to 2 parts of a pediococcus pentosaceus strain A21358, 0.01 to 1.5 parts of a plant lactobacillus strain A21136, 0.05 to 1.5 parts of a lactobacillus rhamnosus strain A21149 and 0.03 to 1.2 parts of a lactobacillus reuteri strain A21325. The probiotic agent comprises the probiotic composition, resistant dextrin, xylooligosaccharide, erythritol, inulin, fructo-oligosaccharide, hericium erinaceus powder, fucoidin and at least one fruit powder selected from grape powder, passion fruit powder and kiwi fruit powder, wherein the specific weight parts of the resistant dextrin, the xylooligosaccharide, the erythritol, the inulin, the fructo-oligosaccharide, the hericium erinaceus powder, the fucoidin and the at least one fruit powder are selected from grape powder, passion fruit powder and kiwi fruit powder. The probiotic agent has the effect of inhibiting helicobacter pylori, and a new thought is provided for clinical related auxiliary treatment of helicobacter pylori infection.

The invention relates to the technical field of microorganisms, in particular to a compound probiotic agent for reducing harmful gases generated in intestinal tracts and regulating intestinal flora. The compound probiotics disclosed by the invention are prepared from bifidobacterium animalis subsp. Lactis Bi66, plant lactobacillus P16 and a saccharomyces boulardii Bld-3 strain. The three strains are compounded and combined for use, so that the content of short-chain fatty acid in intestinal flora can be remarkably increased, the yield of beneficial gases such as CH4 and H2 can be increased, the yield of NH3 and H2S which are harmful to a human body can be reduced, beneficial bacteria can be promoted, and the growth of harmful bacteria can be inhibited, so that the intestinal flora can be regulated. Under the condition of keeping the same amount of the used bacteria, compared with a strain intervention mode without any bacteria, the compounding of the three bacteria has the best effect in the aspect of improving the intestinal flora, which indicates that the three bacteria can be mutually matched and promoted, and can have synergistic interaction in the aspect of regulating the intestinal flora.

The invention provides a method for preparing a biological fermentation heavy metal contaminated soil remediation agent by utilizing ardealite and printing and dyeing sludge, and belongs to the technical field of solid waste recycling. The method comprises the following steps: mixing printing and dyeing sludge, ironmaking solid waste and ardealite, uniformly stirring, granulating, carrying out aging treatment, carrying out high-temperature pyrolysis, and grinding to obtain phosphorus printing carbonized powder; mixing the cordyceps sobolifera mycelium extract, the pandanus elliottii leaf extract and the red velvet extract, and uniformly stirring to obtain an extraction mixture; beta-nicotinamide mononucleotide and galactomannan are mixed and stirred uniformly, and a probiotic agent is obtained; mixing a probiotic agent, the extraction mixture and food powder, and uniformly stirring to obtain enlightening mixed powder; and mixing the heuristic mixed powder with the phosphorus-printed carbonized powder, uniformly stirring, and adding primary rhizosphere compound bacteria for fermentation, thereby obtaining the biological film covering heavy metal contaminated soil remediation agent after fermentation is finished. The efficient utilization of the phosphogypsum and the printing and dyeing sludge is realized through reasonable proportioning, pyrolysis carbonization and primary rhizosphere compound bacteria synergistic fermentation.

The utility model relates to the technical field of traditional Chinese medicine additive processing equipment, in particular to a traditional Chinese medicine additive stirring and mixing device. Comprising a mixing tank, a cover plate and an auxiliary assembly, the auxiliary assembly comprises a stirring motor, a spiral roller, a discharging valve, a temperature sensor, an exhaust component, a feeding component and a heating component, during processing, raw materials are fed into the mixing tank through the feeding component, then the heating component can be used for heating, the temperature sensor is used for detecting internal temperature, the exhaust component can be used for exhausting, and then the stirring motor is started. The stirring motor is controlled to act, the spiral roller can be driven to rotate, automatic mixing is achieved, compared with manual stirring and mixing, the efficiency is higher, the labor intensity of workers can be relieved, finally, materials can be discharged by opening the discharging valve, and therefore automatic stirring and mixing can be achieved, manpower resources are optimized, and the mixing efficiency is improved during probiotic agent processing.

The invention discloses application of bifidobacterium longum subsp. Longum i772 in a product for promoting bone health, and belongs to the field of microbial engineering. The effective components of the product for promoting bone health comprise live bifidobacterium longum subsp. Longum i772 and / or inactivated bifidobacterium longum subsp. Longum i772. The product provided by the invention can effectively promote bone health no matter whether the product contains viable bacteria or inactivated bacteria, and experimental data shows that the probiotic agent containing the bifidobacterium longum subsp. Longum i772 can significantly improve the bone density, bone volume fraction and bone trabecula thickness in a female rat osteoporosis model, and has a good application prospect. The bone trabecula spacing in the female rat osteoporosis model is reduced; meanwhile, the strain has an intestinal colonization effect and can continuously play a role in promoting bone health in the intestinal tract, and the trouble of daily medication can be reduced. The food provided by the invention is suitable for promoting bone health and preventing and / or improving osteoporosis.

The invention discloses a fertilizer containing herbaceous plant components and a preparation method thereof, and particularly relates to the technical field of agricultural waste, the formula is composed of a composite traditional Chinese medicine extract, a core-shell structure carrier, a functional probiotic agent and an auxiliary agent, and the preparation method comprises the steps of S1, graded extraction of traditional Chinese medicines, S2, carrier loading and microcapsule preparation, and S3, probiotic integration and granulation. Through scientific compatibility of the compound traditional Chinese medicine extracts, design of the core-shell structure carrier, directional addition of the functional probiotics and an optimized preparation process, the prepared fertilizer can synergistically improve the growth index and quality of crops, remarkably enhance the disease and pest resistance, effectively improve the physicochemical properties and micro-ecological environment of soil and improve the yield of the crops. The usage amount of chemical pesticides and fertilizers is reduced, integration of nutrition supply, disease prevention and control and soil improvement is achieved, and the requirements of green agriculture development are met.

The invention discloses a probiotic agent for anaerobic biodegradable plastics, a preparation method of the probiotic agent and biodegradable plastics, the probiotic agent takes PBAT as a carrier material and nano starch, PLA, nano calcium carbonate, carbamide and nano fumed silica as probiotic materials, and probiotic particles are formed through multiple mixing and plasticizing granulation. According to the invention, anaerobic degradation can be regulated and controlled. More importantly, anaerobic degradation also realizes normal use of the degradable plastic, the plastic cannot accelerate degradation in light and oxygen environments, the aging property of the plastic is equivalent to that of a resin matrix of the plastic, and the physical and mechanical properties are ensured.

The invention provides a lactobacillus plantarum strain A21337 with the functions of inhibiting bacteria and secreting 5-hydroxytryptamine, a probiotic agent of the lactobacillus plantarum strain A21337 and application of the lactobacillus plantarum strain A21337, and belongs to the technical field of functional microorganisms. The invention provides application of phytobacterium plantarum or a microbial agent containing the phytobacterium plantarum in preparation of a product resisting pathogenic bacteria and / or rich in 5-hydroxytryptamine. According to the invention, a plant lactobacillus strain A21337 is separated from intestinal feces of healthy 100-year old people in Guangxi province, has the characteristics of secreting 5-hydroxytryptamine and inhibiting pathogenic bacteria, and has good tolerance to gastrointestinal fluid. Therefore, the strain A21337 or the probiotic agent containing the strain A21337 provides a new means for preparing a product which is resistant to pathogenic bacteria and / or rich in 5-hydroxytryptamine.

The invention relates to the technical field of tea tree planting, in particular to a tea garden annual management method for effectively preventing and controlling tea cake disease. The invention provides a tea garden annual management method for effectively preventing and controlling tea cake disease. The tea garden annual management method comprises the following steps: respectively spraying a compound probiotic agent in March, May and July; a base fertilizer is applied in November. The annual management method for the tea garden has an obvious growth promoting effect on tea trees, and compared with other control groups, tender leaves of the tea trees are brighter green in color, buds are neater, and the length of the tender leaves and the number of the buds are obviously improved. The method also has an obvious prevention and treatment effect on tea cake disease, and the prevention and treatment effect is obviously superior to that of medicaments in the prior art.

This invention belongs to the field of food fermentation technology, and specifically relates to a compound probiotic agent and its application in the preparation of fermented soy milk. This invention provides a compound probiotic agent composed of *Lactobacillus fermentum* NCPS LF01, *Pediococcus pentosaceus* NCPS PPE01, and *Lactobacillus acidophilus* NCPS LA01 in a volume ratio of 1-2:1:1, and applies this compound probiotic agent to the preparation of soy products, especially fermented soy milk. The beneficial effects of this invention are that fermented soy milk prepared using this compound probiotic agent has a rich flavor, and the total flavonoid and total phenolic content, as well as the in vitro DPPH free radical scavenging rate, are significantly superior to those of ordinary commercially available strains or other strains. In summary, this compound probiotic agent can effectively improve the retention level of active ingredients and antioxidant capacity of fermented soy milk, and has outstanding application value.

The present invention discloses Lactobacillus kefiranofaciens IMAU12319 and its application, belonging to the technical field of microorganisms, and particularly relates to Lactobacillus kefiranofaciens ( Lactobacillus kefiranofaciens ) IMAU12319, which was deposited on July 8, 2024 at the General Microbiological Center of the China Committee for Culture Collection of Microorganisms, with the deposit address being No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the accession number being: CGMCC No. 31224. The application of Lactobacillus kefiranofaciens IMAU12319 in the preparation of probiotic agents resistant to gastrointestinal digestion. The bacterium of the present invention not only exhibits good gastrointestinal tolerance ability, but also shows strong potential in intestinal adhesion, antioxidant and antibacterial aspects, and has broad application prospects in starter cultures.

The invention relates to the technical field of animal feed, and particularly discloses a bamboo powder dietary fiber probiotic feed additive as well as a preparation method and application thereof. The additive consists of bamboo powder dietary fibers, a probiotic agent and a carrier, wherein probiotics are at least one of trichoderma, bacillus, lactic acid bacteria and saccharomycetes. The preparation method comprises the steps of bamboo powder pretreatment, solid state fermentation, drying, mixing and the like. The product fully utilizes the prebiotics characteristics of the bamboo powder and the regulation effect of probiotics, can effectively improve the intestinal health of livestock and poultry, improve the feed utilization rate and reduce the breeding cost, can partially or completely replace antibiotics, and has the characteristics of greenness, safety and high efficiency.