49 results about "Probiotic agent" patented technology

The invention provides a probiotic composition for inhibiting helicobacter pylori as well as a probiotic agent and application thereof, and belongs to the technical field of probiotic functional products. The probiotic composition is prepared from the following components in parts by weight: 0.05 to 2 parts of a pediococcus pentosaceus strain A21358, 0.01 to 1.5 parts of a plant lactobacillus strain A21136, 0.05 to 1.5 parts of a lactobacillus rhamnosus strain A21149 and 0.03 to 1.2 parts of a lactobacillus reuteri strain A21325. The probiotic agent comprises the probiotic composition, resistant dextrin, xylooligosaccharide, erythritol, inulin, fructo-oligosaccharide, hericium erinaceus powder, fucoidin and at least one fruit powder selected from grape powder, passion fruit powder and kiwi fruit powder, wherein the specific weight parts of the resistant dextrin, the xylooligosaccharide, the erythritol, the inulin, the fructo-oligosaccharide, the hericium erinaceus powder, the fucoidin and the at least one fruit powder are selected from grape powder, passion fruit powder and kiwi fruit powder. The probiotic agent has the effect of inhibiting helicobacter pylori, and a new thought is provided for clinical related auxiliary treatment of helicobacter pylori infection.

The invention relates to the technical field of microorganisms, in particular to a compound probiotic agent for reducing harmful gases generated in intestinal tracts and regulating intestinal flora. The compound probiotics disclosed by the invention are prepared from bifidobacterium animalis subsp. Lactis Bi66, plant lactobacillus P16 and a saccharomyces boulardii Bld-3 strain. The three strains are compounded and combined for use, so that the content of short-chain fatty acid in intestinal flora can be remarkably increased, the yield of beneficial gases such as CH4 and H2 can be increased, the yield of NH3 and H2S which are harmful to a human body can be reduced, beneficial bacteria can be promoted, and the growth of harmful bacteria can be inhibited, so that the intestinal flora can be regulated. Under the condition of keeping the same amount of the used bacteria, compared with a strain intervention mode without any bacteria, the compounding of the three bacteria has the best effect in the aspect of improving the intestinal flora, which indicates that the three bacteria can be mutually matched and promoted, and can have synergistic interaction in the aspect of regulating the intestinal flora.

This invention belongs to the field of food fermentation technology, and specifically relates to a compound probiotic agent and its application in the preparation of fermented soy milk. This invention provides a compound probiotic agent composed of *Lactobacillus fermentum* NCPS LF01, *Pediococcus pentosaceus* NCPS PPE01, and *Lactobacillus acidophilus* NCPS LA01 in a volume ratio of 1-2:1:1, and applies this compound probiotic agent to the preparation of soy products, especially fermented soy milk. The beneficial effects of this invention are that fermented soy milk prepared using this compound probiotic agent has a rich flavor, and the total flavonoid and total phenolic content, as well as the in vitro DPPH free radical scavenging rate, are significantly superior to those of ordinary commercially available strains or other strains. In summary, this compound probiotic agent can effectively improve the retention level of active ingredients and antioxidant capacity of fermented soy milk, and has outstanding application value.

This invention relates to the field of probiotic technology, specifically to a combination probiotic agent for improving Alzheimer's disease and its application. The combination probiotic agent includes *Lactobacillus gasseri* LFLG-245 and inactivated *Ackermania maughanii* AKKLF; *Lactobacillus gasseri* (… Lactobacillus gasseri LFLG-245 was deposited on March 24, 2025, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 33952. (Ackermania pseudomallei) Akkermansia muciniphila AKKLF was deposited on March 24, 2025, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 33955. The combined bacterial agent provided by this invention can improve Alzheimer's disease.

The invention discloses a probiotic agent for relieving prediabetic action and application thereof, and belongs to the technical field of microorganisms. Experiments prove that bifidobacterium longum CCFM1113 and bifidobacterium adolescentis CCFM1386 composite probiotics can relieve the prediabetic stage, which is specifically embodied in reducing weight gain of mice in the prediabetic stage, regulating blood sugar metabolism, improving sugar tolerance and insulin resistance, protecting liver and pancreas tissues, being beneficial to increasing the diversity of intestinal flora, recovering the structure of the intestinal flora, and improving the immunity of the mice in the prediabetic stage. The invention also provides a preparation method of the bifidobacterium longum CCFM1113 and bifidobacterium adolescentis CCFM1386 composite probiotics. The bifidobacterium longum CCFM1113 and bifidobacterium adolescentis CCFM1386 composite probiotics. And the growth of beneficial bacteria related to metabolic disorder is enriched and regulated. The invention provides an important basis for developing a dietary formula suitable for prediabetic patients, and provides a new method for prevention and management of diabetes mellitus.

The invention discloses probiotic fermented carrot juice capable of improving intestinal health and resisting oxidation and a preparation method of a metagen. The carrot juice is prepared by fermenting carrot raw materials through a compound probiotic agent containing lactobacillus plantarum YZX21, lactobacillus casei YRL577, lactobacillus paracasei X11 and lactobacillus rhamnosus LR-C111. The preparation method comprises the following steps: cleaning, peeling, crushing and pulping carrots to prepare carrot primary pulp, then carrying out high-temperature instantaneous sterilization treatment on the carrot primary pulp, and carrying out vacuum concentration after sterilization; the concentrated carrot primary pulp is inoculated with the composite probiotic agent, the final inoculation concentration is 102-109 CFU / mL, standing fermentation is conducted for 9-15 hours at the temperature of 30-40 DEG C, the probiotic fermented carrot juice is obtained, and the probiotic fermented carrot juice is subjected to high-temperature sterilization treatment to obtain the postbiotics. The probiotic carrot juice and the metabiotics prepared by the invention have the functions of improving constipation, regulating intestinal flora and improving antioxidant capacity, and the preparation method is stable and efficient.

The application discloses a multi-strain combined solid-state fermentation rapeseed meal probiotic agent and a fermentation method thereof, adopts Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Bacillus subtilis to combine solid-state fermentation rapeseed meal to reduce the content of anti-nutritional factors such as glucosinolates, and comprises the following steps: pretreatment and sterilization of rapeseed meal raw materials, preparation of fermentation seed liquid, solid-state fermentation of probiotics, and preparation of fermented rapeseed meal products. The application firstly uses Bacillus amyloliquefaciens to solid-state ferment rapeseed meal, and combines with Saccharomyces cerevisiae and Bacillus subtilis to add into rapeseed meal to carry out combined fermentation; compared with other technical solutions, the application can greatly reduce the content of anti-nutritional factor glucosinolate in rapeseed meal, and can reduce the content of anti-nutritional factors tannin, phytic acid and crude fiber in rapeseed meal and improve the content of peptides in the rapeseed meal product, various bioactive substances can be produced in the probiotic fermentation process, and the method has the advantages of low cost, simple operation, short time consumption and high efficiency.

The invention discloses a strain of Ligilbacillus murinus PV211184 capable of improving the reproductive performance of livestock, which belongs to the field of agricultural animal husbandry, is preserved in Guangdong Province Microbial Culture Collection Center, has a preservation number of GDMCC No: 66039, has a bacterial colony in milk white forms such as round bulge, smoothness and the like, has a suitable growth temperature of 30-45 DEG C, a pH value of 4.0-6.5, preferably 37 DEG C and a pH value of 6.0, and is subjected to facultative anaerobic culture by using an MRS culture medium. The bacterium can be colonized in the uterus, has good endometrial epithelial cell adhesion capability, has no negative influence on sperm motility and IVF embryonic development, and can promote endometrial epithelial cell proliferation. Experiments show that the litter size per litter of a sow can be increased by 1.3, the litter size per litter can be increased by 1.0, the lambing rate of Hu sheep can be increased by 7.2%, the lambing rate per litter can be increased by 14.5%, and the feed can be used as a probiotic agent for improving the reproductive performance of livestock.

The invention discloses bifidobacterium longum subsp. Infantis i18 with an effect of regulating intestinal flora and application of the bifidobacterium longum subsp. Infantis i18, and belongs to the technical field of microbial engineering. The bifidobacterium longum subsp. Infantis i18 strain is preserved in the China General Microbiological Culture Collection Center, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 25, 2024, the preservation number is CGMCC NO.31411, and the Latin name is Bifidobacterium longum subsp. Infantis. The invention also discloses a preparation method of the bifidobacterium longum subsp. Infantis strain. The bifidobacterium longum subsp. Infantis i18 provided by the invention has a bidirectional regulation effect, can relax bowel, relieve diarrhea, maintain intestinal steady state and regulate intestinal flora at the same time, expands the application range of the bifidobacterium longum subsp. Infantis, and enriches the types of the domestic bifidobacterium longum subsp. Infantis. The preparation method is suitable for preparing the probiotic agent or the infant formula milk powder.

The invention belongs to the technical field of plant planting, and provides a Hainan camellia oleifera high-survival-rate drought-resistant afforestation and biological nutrient solution preparation method. The special nutrient solution for the camellia oleifera comprises the following raw materials in parts by weight: 6-12 parts of a probiotic agent, 8-12 parts of potassium fulvate, 15-20 parts of potassium nitrate, 60-70 parts of a coco coir extract and 95-105 parts of water. Through the synergistic effect of the afforestation method and the special nutrient solution for camellia oleifera, the survival rate and preservation rate of camellia oleifera afforestation are remarkably increased. Wherein the special nutrient solution has high-temperature-resistant, drought-resistant and root-promoting effects, and can effectively promote the growth of roots of camellia oleifera, improve the water-retaining capacity of leaves and increase the content of available phosphorus in soil, thereby enhancing the stress resistance and growth vigor of plants; in the drought period, the leaf wilting degree of the oil-tea camellia adopting the drought-resistant afforestation method is far lower than that of a traditional method, the water content of soil is higher, and stage drought stress can be effectively resisted.

This invention relates to a process for recycling and treating distiller's grains, comprising: pretreatment: solid-liquid separation of distiller's grains to obtain solid material and microbial-containing liquid, adjusting the carbon-to-nitrogen ratio of the solid material to 18-30:1; enzymatic hydrolysis: adding a compound enzyme preparation and an enzyme stabilizer to the solid material for enzymatic hydrolysis to obtain enzymatic hydrolysis products; microbial fermentation: adding a compound probiotic agent to the enzymatic hydrolysis products for fermentation to form fermented material, with microbial-containing liquid introduced during fermentation; grading: grading the fermented material through a vibrating screen to obtain coarse and fine particles; high-temperature treatment: subjecting the coarse particles to high-temperature steam explosion treatment; surface activation: surface modification treatment of the fine particles; secondary fermentation: mixing the high-temperature treated coarse particles with the fine particles, and adding the compound probiotic agent again for secondary fermentation to obtain fermented products; and drying and shaping. This recycling process has high processing efficiency, a shorter fermentation cycle, and higher nutritional content.

The present application relates to tea tree planting technical field, especially effective control tea cake disease occurrence's tea garden annual management method, the present application provides a kind of effective control tea cake disease occurrence's tea garden annual management method, respectively in March, May, July spray compound probiotic agent;In November, base fertilizer is applied.The tea garden annual management method provided by the present application has obvious growth promotion effect on tea tree, compared with other control groups, the color of tender leaf is more tender green, bud is more neat, and the length of tender leaf and the number of bud are obviously improved.The method also has obvious prevention and treatment effect on tea cake disease, and the prevention and treatment effect is obviously better than that of the existing technology.

This invention relates to the field of microbial technology, and particularly to a compound probiotic agent for reducing the production of harmful intestinal gases and regulating intestinal flora. The compound probiotic of this invention consists of *Bifidobacterium animalis* subsp. *lactobacter* Bi66, *Lactobacillus plantarum* P16, and *Saccharomyces boulardii* strain Bld-3. This invention combines these three strains to significantly increase the content of short-chain fatty acids in the intestinal flora, increase the production of beneficial gases such as CH4 and H2, and reduce the production of harmful gases such as NH3 and H2S, thereby promoting the growth of beneficial bacteria and inhibiting the growth of harmful bacteria and regulating the intestinal flora. While maintaining a consistent amount of bacteria, compared with intervention methods lacking any one of the strains, the combination of the three strains showed the best effect in improving the intestinal flora, indicating that the three strains can cooperate and promote each other, and can synergistically enhance the effect in regulating the intestinal flora.

The invention relates to the field of bacterial strains, and discloses a composite probiotic agent and application thereof in improving the flavor of corn puree. According to the composite probiotic agent, sodium alginate and chitosan are subjected to functional group modification to form an embedding material with enhanced hydrophobicity and water solubility, and a pH-sensitive double-layer microcapsule structure is constructed by synergistically embedding plant lactobacillus, lactobacillus mucus fermentation and saccharomyces cerevisiae. The probiotic agent is applied to fermentation of the corn puree, probiotics can be effectively protected to pass through a gastric acid environment, targeted release of intestinal tracts is achieved, meanwhile, the corn puree is promoted to generate rich flavor substances through multi-bacterium synergistic fermentation, and the sweet feeling, milk fragrance and the overall flavor level of a product are remarkably improved. The process conditions are mild, large-scale production is easy, and the obtained corn puree beverage is rich and mellow in flavor and has the probiotic function.

The invention relates to novel paenibacillus with biomacromolecule degradation capacity and nitrogen fixation and phosphorus solubilization effects and application of the novel paenibacillus, the strain is identified as a novel strain of paenibacillus through multi-phase classification, and the novel paenibacillus has the capacity of degrading biomacromolecules such as starch, cellulose, casein, chitin and lecithin. The method can be applied to degradation of related carbohydrates, proteins and lipid substances and preparation of biological enzymes; the bacillus subtilis has the advantages that the bacillus subtilis can be used for preparing the bacillus subtilis, has the phosphorus dissolving capacity of dissolving organic phosphorus and inorganic phosphorus, has nitrogenase and beta-1, 3-glucanase and the activity of generating IAA (Indoleacetic Acid), can play a probiotic role on crops by fixing nitrogen, dissolving phosphorus and inhibiting pathogenic bacteria, can grow in an environment with the salinity not higher than 4.5%, and has certain salt-tolerant activity. Due to multiple functions, the strain can be used for preparing and applying biological nitrogen fertilizers, phosphate fertilizers and crop probiotics, so that the strain plays an important role in crop growth and green agricultural production.

The application provides a lactobacillus gasseri strain A21120 with antioxidant, anti-inflammatory and antitumor functions, and a probiotic agent and application thereof, and belongs to the technical field of functional microorganisms. The strain A21120 is obtained by screening from intestinal feces of a centenarian healthy old person, and is identified by morphology and molecules and belongs to lactobacillus gasseri. The strain has good resistance to gastrointestinal fluid and bovine choline capacity, and has the potential to colonize the intestinal tract. The lactobacillus gasseri strain A21120 provided by the application has bacteriostatic, anti-inflammatory, antioxidant and antitumor activities, and provides a new way for the treatment of clinical related diseases, the development of drugs and the preparation of in vitro preparations or daily necessities.

The invention relates to an application of human-derived fermented lactobacillus mucus TG017 in preparation of a probiotic agent for promoting glucagon-like peptide-1 (GLP-1) secretion, the fermented lactobacillus mucus TG017 is separated from excrement samples of domestic healthy human bodies, the strain is a probiotic, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 2023513. The invention develops a new application of the fermented lactobacillus mucus TG017 strain, and the fermented lactobacillus mucus TG017 has a remarkable effect on improving the secretion level of GLP-1, and has important application value on appetite regulation and weight management of obesity patients.

The invention relates to a solid state fermentation method for improving vinasse feed quality and probiotic functions, which comprises the following steps: substrate pretreatment: drying vinasse until the water content is less than or equal to 12%, crushing, sieving with a 40-mesh sieve, taking 100-120 parts by weight of vinasse powder, adding 20-30 parts by weight of auxiliary materials, uniformly mixing, adjusting the water content to 58-62%, and adjusting the pH to 6.2-6.8 with lime milk; post-treatment: after fermentation is finished, ventilating and drying at 45-55 DEG C until the water content is less than or equal to 10%, crushing and sieving with a 60-mesh sieve to obtain a finished product. According to the invention, through innovative combination of substrate pretreatment optimization, composite auxiliary material synergism, four-strain synergism probiotic agent and two-stage fermentation technology, core defects of natural vinasse and existing fermentation technology are thoroughly solved; the comprehensive effects that the nutritional quality is comprehensively improved, the probiotic functions are multi-dimensionally strengthened, the product stability is remarkably improved, and the application range is greatly widened are achieved.

The invention relates to the technical field of functional foods, and particularly discloses an intestine-moistening and excretion-promoting composition, a compound probiotic preparation and a preparation method of the compound probiotic preparation. The invention provides a composition capable of moistening intestines and promoting excretion. The composition is prepared from the following raw materials in parts by weight: 75 to 150 parts of erythritol, 45 to 90 parts of inulin, 45 to 90 parts of lactitol, 25 to 50 parts of fructus cannabis, 12 to 25 parts of semen raphani, 15 to 30 parts of fructus crataegi, 27 to 55 parts of fructus lycii, 27 to 54 parts of fructo-oligosaccharide, 12 to 25 parts of resistant dextrin and 15 to 33 parts of probiotics. The probiotics are selected from one or more of lactobacillus rhamnosus, pediococcus acidilactici, bifidobacterium animalis, lactobacillus plantarum and lactobacillus reuteri; the invention further provides a compound probiotic preparation prepared from the intestine moistening and excretion promoting composition. The composite probiotic agent provided by the invention can regulate the balance of intestinal flora and promote gastrointestinal motility, and has very good intestine moistening and excretion promoting effects.

The invention discloses a health-care food for enhancing immunity and improving renal function and a preparation method thereof, and the health-care food is prepared from the following raw materials in percentage by mass: 55%-75% of a gel base material, 15%-25% of a fungal polysaccharide fermentation concentrated solution, 3%-6% of a natural antibacterial flavor agent, 1%-3% of a probiotic agent, 0.5%-2% of a nutrient compound, 0.5%-3% of a natural plant extract, 0.3%-0.7% of an acidity regulator, 0.1%-0.5% of a stabilizer and the balance of purified water, wherein the fungal polysaccharide fermentation concentrated solution is a concentrated solution prepared by carrying out lactobacillus plantarum fermentation, inactivation, centrifugation and concentration on inonotus obliquus polysaccharide. The gel type health food provided by the invention has the advantages of good taste, good absorption and multi-target synergistic effect, and can enhance immunity and improve kidney function.

This invention relates to a method for detoxifying and enhancing the aroma of distiller's grains using probiotic solid-state fermentation in the field of distiller's grains reprocessing technology. The method includes the following steps: S1, taking 100 parts by weight of distiller's grains, removing impurities, spreading and drying, adjusting the moisture content to 52-56%, pH to 3.4-3.8, adding 0.8-1.2 parts corn syrup and 0.4-0.6 parts wheat bran, and then mixing evenly; S2, inoculating the pretreated distiller's grains with a 3-4 vol% compound probiotic agent, the total viable bacteria concentration of the agent being ≥1.0 × 10⁻⁶. 9 CFU / mL; S3, In a sealed environment, the inoculated distiller's grains are compacted to a compaction degree of 62-68%, and anaerobic fermented at 30-32℃ for 45-50 hours; S4, The oxygen concentration of the fermentation environment is adjusted to 0.8-1.2 vol%, and solid-state fermentation is carried out at 28-30℃ for 34-38 hours; S5, The product after fermentation in step S4 is dried under negative pressure at 38-42℃ until the moisture content is ≤12%, and then pulverized to obtain the detoxified and aroma-enhanced distiller's grains product. This method solves the problem of mutual inhibition between the detoxification and aroma-enhancing reactions of distiller's grains in current probiotic fermentation.

The invention relates to low-temperature drinking type yoghourt and a preparation method thereof. The low-temperature drinking type yoghourt is prepared from the following raw materials: raw milk, whey protein powder, dried marine algae powder, inulin and a complex microbial inoculant, strain in the complex microbial inoculant comprises streptococcus salivarius subsp. Thermophilus and lactobacillus delbrueckii subsp. Bulgaricus. The whey protein powder, the seaweed meal and the inulin are added into the raw materials for preparing the yoghourt, and meanwhile, the streptococcus salivarius subsp. Thermophilus and the lactobacillus delbrueckii subsp. Bulgaricus are used for carrying out synergistic fermentation on the raw milk; by optimizing the milk source raw materials, the low-temperature drinking type yoghurt with a clean label, high stability, high probiotic activity and fresh and cool taste is prepared.

This invention discloses a cultivation substrate for Dendrobium officinale and its preparation method, relating to the field of cultivation substrate technology. The substrate is an integrated, molded modular structure, including an upper root growth zone and a lower intelligent water guiding system. The root growth zone has a three-dimensional air channel network to optimize gas exchange, a biomimetic rough outer surface to facilitate adhesion, pre-set modular nutrient unit slots for precise fertilization, and uniformly distributed compound probiotic agents to build a healthy rhizosphere. The intelligent water guiding system uses intelligent polymer materials sensitive to environmental humidity and temperature, which can adaptively regulate the water guiding rate to achieve precise and slow water supply. This substrate creates a three-dimensional growth microenvironment with dry and wet separation through the synergistic interaction of water, air, nutrients, and bacteria, thereby significantly improving the survival rate of Dendrobium officinale, promoting robust plant growth and quality improvement, and greatly simplifying cultivation management.

This invention belongs to the field of microbial technology and relates to an anti-infective probiotic agent containing the CT-02 strain, its preparation method, and its application. The strains in the agent include *Streptococcus thermophilus* CT-02 strain (GDMCC No: 64474) and *Lactococcus lactis* subsp. lactis 220007 strain (CGMCC No. 16583). The probiotic agent prepared by this invention can regulate the intestinal barrier and alleviate intestinal infectious inflammation. Furthermore, the *Streptococcus thermophilus* CT-02 strain and the *Lactococcus lactis* subsp. lactis 220007 strain can synergistically enhance the above-mentioned effects.

The invention relates to the field of probiotics, and discloses a composition containing probiotics, a tablet candy and application of the tablet candy. The composite probiotic microbial agent disclosed by the invention comprises bifidobacterium animalis subsp. Lactis BB12, streptococcus salivarius K12 and Witzia coagulans BCN019. In addition to the compound probiotic agent, the composition and the tablet candy disclosed by the invention also comprise medicinal and edible crop powder, and the medicinal and edible crop powder comprises clove powder, chrysanthemum powder and dried orange peel powder. The three specific strains have a synergistic effect and are matched with one another, so that the oral micro-ecological environment can be better improved, the breeding of pathogenic bacteria is inhibited, and the bad breath is improved; besides, the oral health can be improved to a greater extent by utilizing the matching of three kinds of specific medicinal and edible crop powder and the synergistic effect of the medicinal and edible crop powder and the three strains of probiotics.

The invention belongs to the technical field of microorganisms, and discloses associated faecalis and application thereof in preparation of a probiotic agent. The name of the concomitant faecalis is concomitant faecalis HQY061, the preservation number of the concomitant faecalis is GDMCC No: 67255, and the concomitant faecalis is preserved in Guangdong Microbial Culture Collection Center on November 10, 2025. The strain has an obvious inhibiting effect on escherichia coli and staphylococcus aureus, and is suitable for preventing and treating intestinal infectious diseases or regulating intestinal flora; the self-aggregation capability is high, the adhesion to intestinal tracts is facilitated, and the barrier function of the intestinal tracts is enhanced. In addition, the strain shows good safety characteristics, has strong gastrointestinal tract environmental adaptability, and has significant antioxidant activity (antioxidant function). Metabolite analysis shows that the yield of butyric acid is high, and the intestinal barrier and intestinal flora can be improved.

The invention discloses saccharomyces cerevisiae, a probiotic agent and application of the probiotic agent in improvement of animal growth performance, and belongs to the technical field of microbial agents. The saccharomyces cerevisiae is obtained by screening, and is characterized in that the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.20630. The saccharomyces cerevisiae, the bacillus licheniformis B289 and the casei casei Ssd3 are compounded to prepare the probiotic microbial agent, and the probiotic microbial agent is found to be capable of remarkably improving the growth performance, the oxidation resistance, the meat quality and the like of chickens and sheep, so that a theoretical basis is laid for providing a new probiotic microbial agent for livestock and poultry breeding.

The invention discloses a proliferation probiotic composition as well as a preparation method and application thereof, and belongs to the technical field of probiotics. In order to solve the technical problem that an existing probiotic agent is poor in proliferation effect on bifidobacteria and lactobacillus, the invention provides the composition which is prepared from the following components in parts by weight: 1-90 parts of prebiotics, 0.1-20 parts of peptide and 1-20 parts of plant extract and has a remarkable probiotic proliferation effect; the prebiotics are selected from one or more of inulin, stachyose, fructo-oligosaccharide, galactooligosaccharide and isomaltooligosaccharide; the peptide is selected from one or more of casein hydrolysis peptide, collagen peptide and egg white peptide; the plant extract is selected from one or more of euglena powder, fucoidin powder and mushroom powder; the probiotics comprise bifidobacterium or lactobacillus.