55 results about "Intestinal colonization" patented technology

The present invention discloses a fruit and vegetable probiotic tablet and a preparation method thereof. The fruit and vegetable probiotic tablet uses probiotic powders such as lactobacillus plantarum powder as the main raw material, and the preparation method scientifically mixes modified dietary fibers, fruit and vegetable powder, oligosaccharides, plant extracts, protein powder, tea leaf extracts and traditional Chinese medicine extracts and etc., thus improves the content of soluble celluloses which are of real significance for probiotic flora, enhances the physiological activity of celluloses, thereby increases the species of intestinal probiotic flora as well as significantly enhances the colonization ability and time of endogenous and exogenous probiotics in the human intestinal tracts, effectively inhibits the growth and reproduction of harmful intestinal bacteria, especially gram-negative bacteria, and fully regulates the composition of the intestinal probiotic flora. The prepared fruit and vegetable probiotic tablet has a high biological activity, a long human intestinal colonization time, and a significant weight loss effect, and is suitable for a wide range of people.

The invention relates to probiotic solid beverage and a preparation method thereof. The probiotic solid beverage comprises the following raw materials in parts by weight: 24 to 70 parts of maltodextrin, 20 to 60 parts of inulin and 1 to 5 parts of probiotics, wherein the probiotics comprises lactobacillus salivarius, lactobacillus acidophilus, bacillus coagulans, lactobacillus rhamnosus, bifidobacterium bifidum, lactobacillus plantarum and bifidobacterium longum. The probiotic solid beverage provided by the invention has high living bacterium quantity and can reduce loss of the probiotics whenthe probiotics pass through the gastrointestinal tract and increase the survival rate; in addition, the probiotic solid beverage can promote intestinal colonization of the probiotics; moreover, through mutual promotion of the actions of various bacteria, the probiotic solid beverage can adjust intestines and stomach well, promote intestinal tract movement, relieve constipation and diarrhea, reduce serum cholesterol, reduce lactose intolerance, accumulate proteolytic enzyme, adjust nutrient absorption and adjust the immunologic function.

The invention belongs to a nutritive food production field, concretely an intestinal mucosa protection and restoration type enteral nutritive product and a preparation technology thereof, and provides a product capable of protecting intestinal mucosa, promoting restoration of the intestinal mucosa after injury, preventing and mitigating patient constipation, and providing equalization nutrition support. The nutritive product is prepared from micromolecule peptides of sea animals, glutamine dipeptide, soybean peptide, maltodextrin, dietary fiber, and medium- and long-chain triacylglycerol oil, etc., is blended with composite vitamins, prebiotics and probiotics according to nitrogen and heat requirements recommended to medium stress patients, can protect the intestinal mucosa and promote restoration of the intestinal mucosa, and promotes intestinal colonization of beneficial bacteria, thereby inhibiting harmful bacteria proliferation, reducing endotoxin generation, preventing movement of endotoxin, and providing a nitrogen source, carbohydrate, fat and vitamins required by a human body in a pathologic period. The product is suitable for a patient with intestinal mucosa injury caused by a plurality of factors like wound, great surgery operation and tumour chemotherapy, is safe and has no toxic side effect, and can be prepared to be electuary or functional nutritive injectable suspension.

The invention discloses a probiotic pellet preparation and a preparing method thereof. The preparation is a pellet which consists of a probiotic pellet core and an enteric coating layer wrapping the probiotic pillet core. The preparing method comprises the following steps: (1) evenly mixing probiotics and excipients, adding solvents and stirring the mixture to prepare wet material; (2) extruding, cutting and rounding the wet material prepared in step (1) to prepare a spherical wet granule and drying and selecting the probiotic pellet core; and (3) spraying an enteric coating material solution to the probiotic pellet core and drying to obtain an enteric coated pellet core. The probiotic pellet preparation prepared in the method has high intestinal colonization rate and reliable curative effect after being taken orally.

The invention provides a lactobacillus plantarum improving easily-fat body and offspring weight and application thereof, and is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No.14570. The lactobacillus plantarum has excellent acid and bile salt resistance, bile salt hydrolase activity and intestinal colonization ability. Animal experiments and human food experiments show that the lactobacillus plantarum can control weight gain of pregnant women and offsprings caused by a high-fat and high-sugar diet by bile salt hydrolase activity and regulating intestinal flora, at the same time, the lactobacillus plantarum can regulate the intestinal flora, optimize intestinal flora structure, increase number of beneficial bacteria, and improve ratio of bacteroidetes and firmicutes, thereby improving the easily-fat body. The animal experiments and human food experiments show that the lactobacillus plantarum has clear efficacy and high safety, and can reverse the easily-fat body by fundamentally improving the intestinal flora of humans, has a long-lasting non-rebound effect, and has great potential for market development.

The invention relates to a method for measuring the intestinal tract setting capacity of lactobacillus. The method comprises the following steps: 1, collecting excrement samples of a subject who intakes mixed bacterium liquid or bacterium powder; 2, extracting bacterium genomes in the excrement, and obtaining the excrement bacterium genomes; 3, selecting specific fluorogenic quantitative PCR primers, and detecting the amplification specificity of the primers; 4, adopting the bacterium genomes of six types of lactobacillus as templates respectively, and fabricating six types of standard curves between copy numbers of the six types of lactobacillus and ct values respectively; 5, conducting the fluorogenic quantitative PCR, and obtaining the ct values, wherein the amount of each type of lactobacillus in the unit excrement sample can be obtained through conversion; 6, comparing the lactobacillus content of excrement of the subject before and after the subject intakes the mixed bacterium liquid or the bacterium powder, and obtaining the setting capacity of bacterial strains in the intestinal tract. The method is based on the fluorogenic quantitative PCR, the rapid and accurate quantification of the six types of lactobacillus in the excrement samples can be achieved within a short time, and the intestinal tract setting capacity of the lactobacillus can be further compared.

The invention discloses a lactobacillus rhamnosus JYLR-005 as well as a hypoglycemic product and application thereof. The lactobacillus rhamnosus JYLR-005 is preserved in China General MicrobiologicalCulture Collection Center (CGMCC for short) with the preservation number of CGMCC No. 16103. The preserved strain, i.e., the lactobacillus rhamnosus JYLR-005, is prepared into a product for oral administration. The product mainly achieves blood glucose reduction of hyperglycemic population, avoids uncomfortable symptoms caused by drugs to the body, and reduces the recurrent risk of hyperglycemia.The strain is high in gastrointestinal survival and intestinal colonization capability, and can influence generation of various short-chain fatty acids in intestinal tracts to inhibit the activity ofalpha-amylase.

The present invention provides methods and compositions for production of gram-negative bacterial mutants that are defective in intestinal colonization capacity and sensitive to infection by bacteriophage P1. Thus the present invention provides immunogenic compositions for the prevention or attenuation of food- and water-borne illnesses associated with ingestion of bacteria such as enterohemorrhagic Escherichia coli.

The present invention discloses a novel Bifidobacterium DD98 having a 16SrDNA nucleotide sequence shown in SEQ ID No: 1. Further, the Bifidobacterium is preserved in the General Microbial Culture Collection Management Center of China, and a preservation number is CGMCC No. 16573. The Bifidobacterium DD98 of the invention can tolerate higher selenium pressure, can be used for selenium-enriched culture, and has good intestinal colonization ability, and the extracted water-soluble protein has intestinal protection function. The Bifidobacterium DD98 of the invention can alleviate liver damage caused by a high-fat diet and alcohol, and has the protective effect on the liver.

The present invention provides immunogenic compositions against Campylobacter and methods for using the immunogenic composition to generate an immune response against Campylobacter and/or reduce intestinal colonization by Campylobacter.

The invention belongs to the technical field of poultry and livestock culture feed additives, and provides a bacillus subtilis preparation capable of being applicable to intestinal tract fixed implantation and improving digestion rate and immunity. The bacillus subtilis preparation can be fixedly implemented into the animal intestinal tract to improve the microbial flora balance in the host intestinal tract and to achieve the antibacterial and disease prevention effects, can also generate various amylase, cellulase and protease in the animal intestinal tract to improve the animal feed utilization rate. The bacillus subtilis mud and an adsorption agent are mixed according to the mass ratio of 1:9; spray drying is performed to obtain bacterial powder; the adsorption agent uses the total weight of the adsorption agent as the calculation reference, and is prepared by proportionally mixing soluble starch, jerusalem artichoke powder, saccharose and galactose. The feed utilization rate can beimproved; the antibacterial effects are achieved on the intestinal tract pathogenic bacteria; high acid resistance and bile salt tolerance capability are realized; the stable fixed implementation isrealized in the intestinal tract; a novel breeding technology is provided for poultry and livestock industry. The referential breeding technology is provided for other plant varieties difficult to perform normal sexual propagation.

The invention discloses a bacillus. The strain is classified and named as bacillus licheniformis, is numbered as BLCC1-0441 and has been preserved at China Center for Type Culture Collection (CCTCC) on May 7, 2018; the address is Wuhan University, Wuhan, China; its preservation number is CCICC NO.: 2018253. The bacillus is capable of highly yielding alkaline phosphatase as well as efficiently fermenting, has excellent intestinal colonization performance and has an excellent application in chicken breeding.

The invention provides a fermented and expanded healthcare creep feed for suckling piglets and a preparation method of the fermented and expanded healthcare creep feed for the suckling piglets.The feed and the preparation method are technically characterized in that the feed is prepared from, by weight, 10-40 parts of fermented materials, 50-90 parts of granules and 1-2 parts of traditional Chinese medicine accessories; the preparation method includes: sequentially preparing the fermented materials, the granules and the traditional Chinese medicine accessories, mixing and packaging.The fermented and expanded healthcare creep feed and the preparation method have the advantages that by intestinal colonization of probiotics generated in a fermentation process, reproduction of harmful bacteria is inhibited, diarrhea control is realized, and antibiotic consumption is reduced; by expanded granulation, anti-nutrition factors in the feed are effectively damaged, palatability of the feed is improved, and feed waste is reduced; due to addition of Chinese herbal medicines, effective ingredients of the Chinese herbal medicines enable increase of the digestive utilization rate of the feed, and accordingly disease resistance of animals can be enhanced.

The invention discloses a method for genetic modification of intestinal protobacteria based on microencapsulation treatment. The method mainly includes utilizing the method of synthetic biology to genetically transform the selected intestinal primary escherichia coli and then perform microencapsulation treatment. The biosafety synthetic biology technology is combined with the non-invasive optogenetic technique for the treatment of intestinal diseases, and the method has many advantages compared with the traditional methods for treating the intestinal diseases. The intestinal primary escherichia coli are subjected to genetic modification, and the intestinal colonization compliance of the microencapsulated protobacteria is improved in the later stage. A single functional bioactive molecule,that is, a transforming growth factor (TGF-beta1) secreted by the primary escherichia coli is taken as an example to study the application in treatment of inflammatory bowel disease (IBD). The methodprovides a new idea for studying the interaction between enteric microorganisms and the body.

The invention discloses a functional probiotic solid beverage and a preparation method thereof. The functional probiotic solid beverage is prepared from the raw materials inpercentage by mass: 40% ofmaltodextrin, 20% of microcrystalline cellulose, 10% of anhydrous dextrose, 10% of fructo-oligosaccharide, 10% of stachyose, 5% of bifidobacterium longum, 2% of bifidobacterium lactis, 1% of lactobacillus acidophilus, 1% of lactobacillus casei and 1% of lactobacillus rhamnosus. A variety of probiotics (bifidobacterium longum, bifidobacterium lactis, lactobacillus acidophilus, lactobacillus casei and lactobacillus rhamnosus) are combined for use, the product stability is good, the intestinal environment is improved, the human immunity is improved, especially, a significant role is played in thefight against allergic dermatitis, and the intestinal colonization rate and survival rate of the probiotics are greatly improved due to the presence of prebiotics (stachyose and fructo-oligosaccharide).

The invention provides peach gum polyferose which is a peach gum polyferose compound having an iron core center similar to iron proteinate and formed by chelating a peach gum polysaccharide with iron.The peach gum polyferose provided by the invention has beneficial effects that the peach gum polyferose has high iron content and complexation stability; no free Fe<2+> exists in the product; no irritation is caused to the gastrointestinal tract; the iron supplementing effect is good; no toxic substances are used in the production process, and the product has good safety. The adding amount is low. After the iron in the peach gum polyferose is released, the peach gum polysaccharide is a supplement of probiotic nutrition, thereby promoting the biological activity of the probiotic bacteria in intestinal colonization; moreover, the peach gum polysaccharide has a natural function of adsorbing harmful substances, and is also a very good water-soluble dietary fiber, which has good water retention property, can increase the moisture and looseness of feces, and is help to improve the intestinal functions.

The invention discloses a strain of bifidobacterium pseudocatenulatum capable of highly utilizing galactooligosaccharide and application of the bifidobacterium pseudocatenulatum, and belongs to the technical field of microorganisms and the technical field of medicines. The bifidobacterium pseudocatenulatum CCFM1170 obtained by screening in the invention can make high use of the galactooligosaccharide, which is specifically embodied in that: after the intervention of the galactooligosaccharide, the relative abundance of the strain in the intestinal tract of a source host is obviously improved; the strain shows stronger applicability, faster growth rate and shorter generation time in an in-vitro YCFA culture medium taking galactooligosaccharide as a carbon source, namely an intestinal microorganism in-vitro simulation culture method; according to the bifidobacterium pseudocatenulatum CCFM1170 disclosed by the invention, the galactooligosaccharide is highly utilized, so that the competitive adaptability of the bifidobacterium pseudocatenulatum CCFM1170 during intestinal colonization is improved; and the colonization strength and durability in the intestinal tract of a mouse are remarkably improved. Therefore, the bifidobacterium pseudocatenulatum CCFM1170 has a huge application prospect in the preparation of a product which has high colonization capability and is used for improving the health of the host.

The invention relates to bifidobacterium lactis JYBR-390 with a constipation treatment effect as well as an application and product of the bifidobacterium lactis JYBR-390. The bifidobacterium lactis JYBR-390 is preserved in China General Microbiological Culture Collection Center on July 8, 2019, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC NO.18093, and the bifidobacterium lactis JYBR-390 is classified and named as Bifidobacterium lactis; and the bifidobacterium lactis JYBR-390 is used for preparing a product for treating constipation. The bifidobacterium lactis JYBR-390 has the characteristics of strong intestinal colonization capability and pathogenic bacterium inhibition capability by determining a constipation action mechanism, so that the product for treating constipation is prepared, and the product has an obvious bowel relaxing effect.

The invention provides a method for genetically modifying intestinal primitive bacteria. Screened intestical primitive escherichia coli are genetically modified by a synthetic biology method and thenare microencapsulated. Mucin (Ag43) which is single functional bioactive molecule secreted by the primitive escherichia coli is taken as an example, and the application of mucin (Ag43) in the treatment of an inflammatory bowel disease (IBD) is studied. The invention provides a new idea for researching the interaction between intestinal microorganisms and organisms. A bio-safe synthetic biologicaltechnology and a non-invasive photogenetic technology are combined to be applied to the treatment of intestinal diseases, and compared with a traditional intestinal disease treatment method, the method has the advantages that the screened intestinal primative escherichia coli are genetically modified, so that the biological compliance of intestinal colonization of microencapsulated primitive bacteria in the later period is improved. A synthetic biological technology is combined with a photogenetic technology, so that bioactive molecules for treating the intestinal diseases can be accurately regulated and controlled in time and space.

The invention discloses a microbial preparation for promoting the growth of an intestinal micro-ecological environment of infants, and aims to solve the technical problem that the infants fed by formula milk products cannot obtain beneficial microbial florae in breast milk. The microbial preparation disclosed by the invention is prepared from the following components in parts by mass: 35 to 40 parts of bifidobacterium, 35 to 40 parts of lactobacillus, 0.5 to 1.5 parts of vitamin, 10 to 20 parts of lactose, 5 to 10 parts of calcium lactate and 5 to 10 parts of fructo-oligosaccharide. The microbial agents in the microbial preparation is combined with an intestinal mucosa epithelium surface specific receptor to form a fixed pellicle structure, and intestinal colonization probiotics are formed; intestinal wall absorption and secretion functions are maintained; and vitamin, carbohydrate and calcium supplement each other and act together to promote the growth and development of babies.

The present disclosure provides vaccine compositions for use against enterotoxigenic Escherichia coli (ETEC) comprising EtpA and EatA. EtpA and EatA are prevalent amount diverse ETEC isolates and display significant sequence conservation. In an aspect, the disclosure encompasses a vaccine composition comprising EtpA and EatA. In another aspect, the disclosure encompasses a method of protecting against intestinal colonization of enterotoxigenic Escherichia coli (ETEC) in a subject. The method comprises administering to the subject an effective amount of a vaccine composition comprising EatA and EtpA.

A bacillus licheniformis/traditional Chinese medicine polysaccharide composition and preparations thereof, which are widely used in the technical field of microorganism, are disclosed. The composition comprises bacillus licheniformis, traditional Chinese medicine polysaccharides and auxiliary materials. The bacillus licheniformis relates to bacillus licheniformis CMCC63516 living bacteria powder. The weight ratio of the bacillus licheniformis CMCC63516 living bacteria powder to a traditional Chinese medicine extract product is 1:1.25-25. The traditional Chinese medicine polysaccharides are one of lycium barbarum polysaccharides, lentinan, astragalus polysaccharides, codonopsis pilosula polysaccharides, and acanthopanax senticosus polysaccharides. The polysaccharide content in the traditional Chinese medicine polysaccharides is 70-95%. In aerobic and anaerobic conditions, each traditional Chinese medicine polysaccharide has a multiplication effect for the bacillus licheniformis, and the multiplication effect of the traditional Chinese medicine polysaccharides is associated with the adding amount in a seed culture medium. The composition is prepared into an oral preparation and prepared into common tablets. The composition promotes significant multiplication of the bacillus licheniformis under aerobic and anaerobic conditions, enhances the capability of restoring intestinal flora balance, and rapidly enhances the intestinal colonization resistance. The composition is rapid to take effect and short in course of treatment when treating diarrhea and other intestine diseases.

The invention relates to a preparation method and application of a probiotic spore Capsid Nano-materials and probiotic composition. The problem that various gastric acid-intolerant probiotics are inactivated or reduced in activity after being orally taken through the stomach, and then the intestinal colonization efficiency is low can be effectively solved, growth and proliferation of harmful bacteria are inhibited, the intestinal barrier is recovered and rebuilt, the intestinal flora is adjusted, and then the colon cancer related to colitis and colitis is effectively prevented and treated. Thepreparation method comprises the steps that firstly, probiotics are cultured to generate spores, then spore Capsid is extracted through an extruder and prepared into the Capsid Nano-materials (CNMs)uniform in particle size and stable in property, then the Capsid Nano-materials and the probiotics are co-incubated in a growth culture medium, and the spore Capsid Nano-materials and probiotic composition is obtained. The preparation method is simple, low in production cost and stable in gastric acid environment, the intestinal flora is adjusted, the intestinal barrier is enhanced, and a new technical support is provided for treatment of inflammatory bowel diseases and inflammation induced cancers.

The invention belongs to the technical field of food, and particularly relates to a gastrointestinal tract controlled release type probiotic bead. The probiotic bead comprises a flowing state oil phase with dispersed bacteria, a solid lipid protection layer and a gel phase; a supercooling medium is released in the dropping process of the probiotic bead, so that the molten solid fat is instantly cooled to form a solid lipid protection layer on the outer layer of the flowing state oil phase; solid particles such as silicon dioxide are added into the solid lipid protection layer, so that holes in the gel phase can be supplemented in time when a large number of hole structures are generated by enzymolysis in the intestinal digestive tract, and the structural integrity of the probiotic bead is kept; and in the gel phase, a condensation-induced network gel technology is adopted, and whey protein isolate gel is induced by edible ethanol, so that the gel phase with a more compact structure can be formed. The probiotic bead enriches the form of probiotic products, realizes high entrapment efficiency of the probiotics and high survival rate of gastrointestinal tracts, and delivers the probiotics to the colon to complete intestinal colonization.