Lactobacillus rhamnosus JYLR-005 as well as hypoglycemic product and application thereof

A Lactobacillus rhamnosus, hypoglycemic technology, applied in the direction of Lactobacillus, application, bacteria, etc., can solve problems such as the production of undesirable ingredients and potential safety hazards, and achieve the effects of avoiding discomfort symptoms, reducing overdose, and optimizing taking time

Active Publication Date: 2018-12-21
WEIFANG ZHONGKEJIAYI BIO FODDER TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the release of active ingredients may be accompanied by the production of undesirable ingredients, which may cause safety hazards to other parts of the body while curing the disease

Method used

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  • Lactobacillus rhamnosus JYLR-005 as well as hypoglycemic product and application thereof
  • Lactobacillus rhamnosus JYLR-005 as well as hypoglycemic product and application thereof
  • Lactobacillus rhamnosus JYLR-005 as well as hypoglycemic product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Collect feces of healthy young adults without family history of hyperglycemia, and isolate lactic acid bacteria strains

[0031] ①Bacteria source: feces of healthy young adults without family history of hyperglycemia

[0032] ② Plate medium: peptone 10g beef powder 5g yeast powder 4g K 2 HPO 4 ·7H 2 O 2g triammonium citrate 2g sodium acetate 3H 2 O 5g glucose 20g Tween 80 1mL MgSO 4 ·7H 2 O 0.2g

[0033] MnSO 4 4H 2 O 0.05g agar 15g distilled water 1000mL

[0034] ③Isolation of strains

[0035] In Bama County, Guangxi Province, the feces of young and middle-aged adults with no family history of blood sugar were sampled, and the samples were sampled with a fecal sampler. During the sampling process, gloves were worn for disinfection to avoid contact with the samples to prevent contamination. Store in a refrigerator immediately after taking it out, and record it with a number.

[0036] The sample liquid was diluted tenfold, and 100 μL of each gradient was ev...

Embodiment 2

[0046] Survival of strains through simulated gastrointestinal digestive fluid

[0047] Preparation of simulated gastric juice: take 16.4mL of 9.5% hydrochloric acid, dilute with distilled water to make the pH value reach 2.0, add 1.0g of pepsin per 100mL, mix well, filter through a 0.22μm sterile filter membrane, and prepare and use immediately. Take 1g of 10 billion / g bacterial powder of JYLR-005 strain, transfer it to a preheated test tube filled with 9mL simulated gastric juice, and treat it at 37°C and 80r / min for 1h, 2h, 3h. The sample solution was serially diluted, counted by pouring method, and each treatment was repeated three times.

[0048] Preparation of simulated intestinal juice: take KH 2 PO 4 6.8g, add 500mL distilled water to dissolve, adjust pH to 6.8 with 4g / L NaOH solution, distill water to 1L, add bile salt 0.3%, add 1g trypsin according to 100mL, mix well, filter with 0.22μm sterile filter membrane, now Ready to use. Take 1g of 10 billion / g bacterial ...

Embodiment 3

[0055] Control Experiment of Adhesion Rate of Different Strains to Caco-2

[0056] Number of sticks at 40 fields of view

[0057] Reagent

[0058] MRS medium: peptone 10.0g, beef powder 5.0g, yeast powder 4.0g, glucose 20.0g, Tween 80 1.0mL, K 2 HPO 4 ·7H 2 O 2.0g, sodium acetate·3H 2 O 5.0g, triammonium citrate 2.0g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 4H 2 O0.05g

[0059] DMEM medium (purchased from GIBCO company)

[0060] PBS 1L formulation pH7.4: Potassium dihydrogen phosphate (KH 2 PO 4 ): 0.27g, disodium hydrogen phosphate (Na 2 HPO 4 ): 1.42g, sodium chloride (NaCl): 8g, potassium chloride (KCl) 0.2g, add about 800mL of deionized water and stir to dissolve, then add concentrated hydrochloric acid to adjust the pH to 7.4, and finally dilute to 1L

[0061] sticky operation

[0062] Caco-2 cells were placed in DMEM medium containing 10% heat-inactivated newborn calf serum and double antibodies (penicillin concentration 100 U / mL, streptomycin 1.0 μg / mL), at 37°C, 5% ...

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Abstract

The invention discloses a lactobacillus rhamnosus JYLR-005 as well as a hypoglycemic product and application thereof. The lactobacillus rhamnosus JYLR-005 is preserved in China General MicrobiologicalCulture Collection Center (CGMCC for short) with the preservation number of CGMCC No. 16103. The preserved strain, i.e., the lactobacillus rhamnosus JYLR-005, is prepared into a product for oral administration. The product mainly achieves blood glucose reduction of hyperglycemic population, avoids uncomfortable symptoms caused by drugs to the body, and reduces the recurrent risk of hyperglycemia.The strain is high in gastrointestinal survival and intestinal colonization capability, and can influence generation of various short-chain fatty acids in intestinal tracts to inhibit the activity ofalpha-amylase.

Description

technical field [0001] The invention relates to the technical field of probiotics and their applications, in particular to a Lactobacillus rhamnosus JYLR-005 and its hypoglycemic products and applications. Background technique [0002] Diabetes is a group of metabolic diseases characterized by hyperglycemia. At present, there are mainly the following treatment methods: [0003] Western medicine treatment: mainly insulin and oral hypoglycemic drugs, roughly divided into sulfonylureas, biguanides, traditional Chinese medicine preparations, other hypoglycemic drugs and adjuvant drugs to control blood sugar rise, relieve symptoms of diabetes, delay further deterioration of diabetes and The emergence of complications. However, long-term use of such drugs will have strong side effects. [0004] Traditional Chinese medicine treatment: Traditional Chinese medicine treatment of diabetes, like other diseases, is characterized by the emphasis on individual differences in patients an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135C12R1/225
CPCA23L33/135A23V2002/00C12R2001/225C12N1/205A23V2400/175A23V2200/328
Inventor 潘玉林司书锋曹维超马明磊闫洪伟吴云杰
Owner WEIFANG ZHONGKEJIAYI BIO FODDER TECH
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