78 results about "Lactobacillus rhamnosus" patented technology

The application discloses application of lactobacillus rhamnosus in preparation of a medicine for treating osteoporosis, relates to the technical field of biological medicine, and the osteoporosis is osteoporosis caused by estrogen deficiency; the medicine is used for inhibiting osteoclast differentiation to play the role of improving osteoporosis; the lactobacillus rhamnosus in the medicine is used for inhibiting the generation of active oxygen to inhibit osteoclast differentiation, thereby improving the osteoporosis caused by estrogen deficiency; the lactobacillus rhamnosus in the medicine is used for activating a nuclear factor Nrf2 signal path and downstream antioxidant enzymes to play an antioxidation stress role; the lactobacillus rhamnosus in the medicine is used for activating an Nrf2-mediated antioxidation path, inhibiting active oxygen generation, and then hindering osteoclast differentiation and function, thereby playing the role of improving osteoporosis; the lactobacillus rhamnosus provides a brand-new medicine treatment strategy for osteoporosis.

A starter culture for the fermentation of a plant-based milk alternative is provided, which is particularly suitable for the production of a plant-based yogurt alternative. The starter culture comprises lactic acid bacteria isolated from legume plant material, especially pea plant material. Preferably, the starter culture comprises lactic acid bacteria of the species Lactobacillus rhamnosus and / or Lactococcus lactis subsp. cremoris.

This invention discloses a probiotic liquid core ball cultured under polysaccharide confinement, its preparation method, and its application in improving children's intestinal flora. Belonging to the field of probiotic preparation technology, it employs an extrusion method combined with calcium alginate cross-linking technology, using xanthan gum and other thickening polysaccharides to optimize the types and concentrations of polysaccharides to construct the confinement space. The effects of different oxygen conditions on the encapsulation of different proportions of *Lactobacillus rhamnosus* LRa05 and *Bifidobacterium animalis* BLa80 were investigated to obtain the optimal probiotic liquid core ball cultured under polysaccharide confinement. This process is simple, uses safe raw materials, and after confinement culture, the viable bacteria count is ≥10⁹ CFU / g. It can directionally increase the abundance of beneficial bacteria in children's intestines, inhibit opportunistic pathogens, enhance flora diversity and stability, and is suitable for children's dietary scenarios, providing a safe and efficient intestinal flora regulation solution.

This invention discloses a strain of *Lactobacillus rhamnosus* H7 and its applications, belonging to the field of food microbiology technology. This invention provides a *Lactobacillus rhamnosus* H7 isolated from fermented meat, deposited at the Guangdong Provincial Microbial Culture Collection Center. This strain has the function of degrading nitrite. When inoculated into dried sausage, the residual nitrite and nitrosamine levels in the dried sausage are reduced, and the color of the meat product is significantly improved. When inoculated into fermented mandarin fish, the residual nitrite and nitrosamine levels in the fermented mandarin fish are also reduced. Therefore, inoculating fermented meat products with *Lactobacillus rhamnosus* H7 not only helps improve the safety of fermented meat products but also enhances their quality. This strain has good application prospects as a starter culture for fermented meat products.

The present disclosure relates to a use of Lacticaseibacillus rhamnosus FMBL L23004 CNN in the preparation of a medicament for preventing or treating diabetes mellitus. The Lacticaseibacillus rhamnosus FMBL L23004 CNN was deposited with the China Center for Type Culture Collection (CCTCC) on 26 Jun. 2023 under the accession number CCTCC NO: M 20231099. The Lacticaseibacillus rhamnosus FMBL L23004 CNN strain has the effect of inhibiting the activity of α-glucosidase, α-amylase, and dipeptidyl peptidase IV. Administration of the strain can significantly lower oral glucose tolerance and insulin tolerance in Type 2 Diabetes Mellitus (T2DM) mice, reduce abnormal postprandial blood glucose fluctuations, decrease fasting blood glucose levels, lower fasting insulin levels, reduce blood lipid levels, and alleviate insulin resistance. The strain significantly improves the composition of the gut microbiome, increases the abundance and diversity of beneficial gut bacteria, and reduces the relative abundance of pathogenic bacteria.

The present application discloses a use of lactobacillus rhamnosus TCI366 and / or its metabolite, which is a use of lactobacillus rhamnosus TCI366 and / or its metabolite in preparation of a composition for improving skin condition, wherein the lactobacillus rhamnosus TCI366 is deposited in the German Collection of Microorganisms and the deposit number is DSM33290.

The application discloses a probiotic composition and a production process of a probiotic fermented bread crumb, and belongs to the technical field of bread crumb processing. The probiotic composition is a microcapsule structure containing malt dextrin, arabic gum and heat-denatured whey protein isolate as wall materials, and lactobacillus acidophilus and lactobacillus rhamnosus as core materials. The structure can ensure that a small amount of live bacteria and metabolic products are slowly released through the micropores of the wall materials in a normal temperature fermentation environment, so that the free lactic acid bacteria are prevented from rapidly proliferating, excessively producing acid and competing with yeast for carbon and nitrogen sources in the initial stage of fermentation. The probiotic composition can provide nutrition for the yeast in the fermentation process, promote cell division and metabolic activity of the yeast, and the polydextrose, inulin and arabic gum in the microcapsule wall materials are hydrophilic colloids, which can form a uniform hydration network in the dough.

This invention discloses a kidney-tonifying and liver-nourishing compound probiotic Cistanche fermented drink and its preparation method, comprising the following steps: mixing and sterilizing Cistanche deserticola, Poria cocos, malt, functional components, and natural selenium-strontium mineral water to obtain a fermentation substrate; activating *Lactobacillus casei*, *Lactobacillus acidophilus*, *Lactobacillus rhamnosus*, and *Lactobacillus plantarum* strains respectively, centrifuging and freeze-drying, and then mixing them to obtain a compound probiotic powder; culturing the compound probiotic powder in MRS medium to obtain a compound probiotic liquid; inoculating the compound probiotic liquid into the fermentation substrate for fermentation, sterilizing, and obtaining the kidney-tonifying and liver-nourishing compound probiotic Cistanche deserticola fermented drink. This invention, through the innovative integrated formulation technology of combining Cistanche deserticola, malt, and Poria cocos as medicinal and edible ingredients, multifunctional factor compounding, and compound probiotic fermentation, prepares a kidney-tonifying probiotic Cistanche deserticola fermented drink with high active ingredients, clear efficacy, and excellent flavor.

A Lacticaseibacillus rhamnosus MBP01 and a combination thereof for preventing and treating irritable bowel syndrome are provided, which relates to the technical field of microorganism. A deposit number of the Lacticaseibacillus rhamnosus MBP01 is GDMCC NO: 64725. The MBP01 can improve a weight loss trend of IBS model mice, increase food intake and feces water content, alleviate colon tissue damage, downregulate expression levels of IL-1β, TNF-α, PAR-2, and serum CORT and increase an expression level of IL-10. MBP01 with a high concentration can improve an expression level of OCC. Therefore, MBP01 has a certain positive regulatory effect on the intestinal function of IBS model mice and can effectively prevent and treat irritable bowel syndrome.

The application discloses a cat oral cavity nursing probiotic composition and application thereof, and the probiotic composition comprises animal bifidobacterium and lactic acid bacteria, wherein the lactic acid bacteria is any one or a combination of multiple of salivarius, lactobacillus casei, enterococcus faecalis, lactobacillus rhamnosus, lactobacillus plantarum, lactobacillus acidophilus and lactobacillus reuteri; compared with single probiotic, the probiotic composition has better effect on oral cavity odor removal, can effectively improve cat oral cavity source halitosis, and can also reduce dental plaque formation, relieve gingivitis and dental caries, and can be applied to preparation of a cat oral cavity odor cleaner, and has good application prospect.

The present invention relates to a Lactobacillus rhamnosus strain. In particular, the present invention relates to a composition comprising a Lactobacillus rhamnosus optionally in combination with a further lactobacilli and the use of the same in the treatment, prevention or alleviation of candidiasis.

A probiotic composition for treating or preventing metabolic dysfunction-associated steatotic liver disease-associated hepatocellular carcinoma(MASLD-HCC) or colorectal cancer, comprises one or more microbiota selected from a group consisting of Lactobacillus helveticus, Lactobacillus plantarum, Lactobacillus rhamnosus GG, Lactobacillus paracasei, Lactobacillus acidophilus, Bifidobacterium breve, Bifidobacterium animalis subsp. Lactis, Streptococcus thermophilus or any combination thereof. The probiotic composition demonstrates improved therapeutic effects when combined with low-dose Sorafenib for MASLD-HCC and outperforms 5-Fluorouracil in colorectal cancer models.

The present invention provides compositions and methods for treating patients. [Solution] The exemplary composition may include a vaginal microbiome composition. The vaginal microbiome composition may include a mixture of bacteria. The bacterial mixture may include Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus jensenii, and Lactobacillus rhamnosus.

The application discloses a 3D printing bacterial cellulose-based Lactobacillus rhamnosus / cottonseed peptide embedding material and a preparation method and application thereof. The method comprises the following steps: mixing sodium alginate and bacterial cellulose after shearing and homogenizing to obtain a sodium alginate-bacterial cellulose mixed solution, then adding Lactobacillus rhamnosus powder and cottonseed peptide to form a uniform suspension, and dropping the uniform suspension into a calcium chloride solution to solidify and form microspheres; another sodium alginate-bacterial cellulose mixed solution with high bacterial cellulose content is prepared as 3D printing ink, the microspheres are uniformly dispersed in the ink, and then 3D printing is performed, finally, a calcium chloride solution is sprayed on the surface of the printing product for secondary solidification, and a composite material with a double-layer embedding structure is obtained. The double-layer embedding system constructed by the bacterial cellulose and the sodium alginate can effectively protect and control the release of the Lactobacillus rhamnosus and the cottonseed peptide, and significantly improves the treatment effect of enteritis.

This invention discloses a primer-probe combination for detecting multiple types of Lactobacillus and its applications, belonging to the field of biotechnology. The primer-probe combination includes a primer-probe combination for detecting *Lactobacillus casei*, a primer-probe combination for detecting *Lactobacillus paracasei*, and a primer-probe combination for detecting *Lactobacillus rhamnosus*. The primer-probe combination of this invention can be used for both singleton and multiplex PCR detection, and can accurately detect *Lactobacillus casei*, *Lactobacillus paracasei*, and *Lactobacillus rhamnosus*, enabling the authentication of products containing Lactobacillus. This invention provides a more convenient method for detecting *Lactobacillus casei*, *Lactobacillus paracasei*, and *Lactobacillus rhamnosus*.

This invention discloses the application of *Lactobacillus rhamnosus*-fermented ginseng freeze-dried powder in the preparation of immunomodulatory drugs, belonging to the field of bio-fermentation and immunomodulation technology. The *Lactobacillus rhamnosus*-fermented ginseng freeze-dried powder is prepared by fermenting ginseng with *Lactobacillus rhamnosus*. The rare ginsenosides include at least one of Ck, Rk1, Rh4, and Rg5. The immunomodulatory drug is used to improve cyclophosphamide-induced immunodeficiency. The amount of *Lactobacillus rhamnosus*-fermented ginseng freeze-dried powder added to the immunomodulatory drug is 10%-50% (mass fraction). The preparation method of the *Lactobacillus rhamnosus*-fermented ginseng freeze-dried powder is as follows: S1, ginseng pretreatment; S2, strain activation; S3, fermentation culture; S4, freeze-dried powder preparation. This invention has the technical effects of high bioavailability of ginseng active ingredients, significant immunomodulatory effect, good safety, and effective improvement of cyclophosphamide-induced immunodeficiency.

An oat preparation, an oat product comprising the oat preparation, a preparation method therefor and the use thereof. The oat preparation is obtained by fermenting a fermentation broth; the fermentation broth comprises a fermentation substrate and a starter culture; the fermentation substrate mainly consists of hydrolyzed oat bran; the starter culture comprises lactobacillus reuteri, lactobacillus plantarum, lactobacillus acidophilus, bifidobacterium lactis and lactobacillus rhamnosus, and comprises streptococcus salivarius subsp. thermophilus and lactobacillus delbrueckii subsp. bulgaricus.

The application discloses a compound microbial agent for improving fiber utilization of broilers and application thereof, and belongs to the technical field of microorganisms. The compound microbial agent is compounded by Lactobacillus casei R7-6 and Lactobacillus rhamnosus R5-8 at a volume ratio of 1:0.5-2. The two known strains are compounded for the first time, and it is found that the compounded strains have a synergistic effect within the range of 1:0.5-2, and the effect is significantly reduced beyond the range. The compound microbial agent has selective fermentation characteristics for different fiber source substrates, and the fermentation effect of the compound microbial agent on purple elephant grass and Guimin elephant grass is significantly better than that on alfalfa and Gegen. After fermentation, the content of crude protein is increased, and the content of neutral detergent fiber is reduced. When the compound microbial agent is used for feeding the purple elephant grass or the Guimin elephant grass, the daily average weight gain of broilers is increased, the feed conversion ratio is reduced, and the abdominal fat rate is reduced. The application provides an efficient microbial solution for high-value utilization of agricultural by-products such as purple elephant grass, Guimin elephant grass and other fibers.

This invention belongs to the field of food technology, specifically relating to a fermented tomato cube and its preparation method. The preparation method includes the following steps: (1) Peel and seed fresh tomatoes, cut them into cubes, sterilize them, and place them in a sterile fermentation container. First, add *Lactobacillus casei*, mix evenly, and anaerobic ferment. Then, add *Lactobacillus rhamnosus*, mix evenly, and continue anaerobic fermentation. After sterilization, fermented tomato cubes are obtained; (2) Wash and peel onions, cut them into cubes, and pre-cook them to obtain pre-cooked onion cubes; (3) Mix the fermented tomato cubes, pre-cooked onion cubes, and seasonings evenly, cook at 80-85℃ for 20-25 minutes, and cool to room temperature to obtain fermented tomato cubes. The preparation method of this invention can ensure the efficient increase of total phenols in fermented tomato cubes while reducing the degradation of lycopene, achieving synergistic optimization of the nutritional quality of fermented tomato cubes, and improving the sensory quality of the final product.

This invention, entitled "A Sunflower Pollen Fermentation Product, Its Preparation Method, and Its Application," belongs to the field of biotechnology. The technical problem to be solved is to improve the antioxidant, immunomodulatory, and intestinal protective capabilities of sunflower pollen. The key technical solution is the preparation method, which includes the following steps: adding sunflower pollen to a fermentation culture medium to obtain a fermentation substrate; inoculating the fermentation substrate with *Lactobacillus* for fermentation culture, separating the solid and liquid phases to obtain a supernatant; concentrating and drying the supernatant to obtain the final product. The *Lactobacillus* is selected from *Lactobacillus casei*, *Lactobacillus plantarum*, and *Lactobacillus rhamnosus*.

The present application belongs to the technical field of biological fermentation, and relates to a spirodelated sophora ferments, a preparation method thereof and application of the spirodelated sophora ferments in preparation of anti-wrinkle firming and soothing care products. The spirodelated sophora, glucose, dipotassium hydrogen phosphate and water are mixed, and after enzyme inactivation and sterilization, a fermentation culture medium is prepared; Lactobacillus plantarum and Lactobacillus rhamnosus are mixed and inoculated into the fermentation culture medium, and then fermentation is carried out in an anaerobic environment. The present application utilizes two strains to ferment spirodelated sophora, which not only has mild conditions, can avoid high-temperature damage to active ingredients such as total phenol, polysaccharide, flavone and amino acid, avoids the addition of organic solvents, and significantly improves the content of active ingredients such as total phenol, polysaccharide, flavone and amino acid, which is conducive to industrial production. At the same time, the fermentation liquor has the effects of anti-wrinkle firming, antioxidant, soothing and care.

This invention belongs to the field of ulcerative colitis treatment, specifically relating to the application of extracellular vesicles derived from *Lactobacillus rhamnosus* in alleviating colitis. This invention discloses the application of extracellular vesicles derived from *Lactobacillus rhamnosus* in the preparation of drugs for alleviating colitis: the extracellular vesicles derived from *Lactobacillus rhamnosus* are extracted from the supernatant of the fermentation broth of *Lactobacillus rhamnosus*, which is classified as *Lactobacillus rhamnosus* (…). Lactobacillus rhamnosus ZFML003, with accession number CCTCC NO: M 2025329. The extracellular vesicles derived from *Lactobacillus rhamnosus* of this invention can inhibit *Ackermania* spp. (…). Akkermansia Excessive proliferation of [organisms]; can be used to develop drugs for the prevention, relief or treatment of colitis.

The application belongs to the technical field of microorganisms, and relates to a preparation method of lactobacillus rhamnosus powder, a product and application thereof. The preparation method comprises the following steps: inoculating lactobacillus rhamnosus into a fermentation medium, fermenting, obtaining a fermentation liquor, centrifuging the fermentation liquor to obtain a bacterial slurry, mixing the bacterial slurry with a composite protective agent, and freeze-drying to obtain the lactobacillus rhamnosus powder. The composite protective agent comprises trehalose, malt dextrin, sodium octenyl succinate starch, inulin, L-arginine and L-proline, and the solvent is water. The lactobacillus rhamnosus powder prepared by the method has a high number of viable bacteria and can be applied to fermented food.

This invention provides a composite composition containing nervonic acid and its use in brain nerve repair, belonging to the field of biotechnology. The invention uses nervonic acid and phosphatidylserine, combined with plant extracts fermented by a microbial concoction of *Lactobacillus acidophilus*: *Lactobacillus rhamnosus*: *Bacillus belyssae* = (2-3):1:1, to form a composite composition. This scientific formulation achieves synergistic effects from multiple components, improving neurological deficits, reducing cerebral infarction volume, decreasing the release of neurotrophic markers, and upregulating neurotrophic factor expression through multi-target synergistic effects. Furthermore, this synergistic effect depends on specific component ratios, providing crucial experimental support for the development of subsequent brain-protective functional compositions. This composition can be widely applied to the preparation of brain nerve repair drugs, functional foods, and dietary supplements, possessing promising industrialization prospects and clinical value.