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Lactobacillus rhamnosus, lactobacillus rhamnosus preparation and application thereof

A technology of Lactobacillus rhamnosus and uses, applied in the field of microorganisms, can solve problems such as inability to achieve beneficial effects, and achieve the effects of wide application forms, reduction in quantity, and reduction in the incidence of periodontal disease

Active Publication Date: 2018-05-18
河北一然生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the definition of probiotics in the world usually refers to microbial strains that are alive, have sufficient quantities, and are beneficial to the host to be called probiotics. Sustained activity and in sufficient quantities, otherwise the beneficial effect cannot be achieved

Method used

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  • Lactobacillus rhamnosus, lactobacillus rhamnosus preparation and application thereof
  • Lactobacillus rhamnosus, lactobacillus rhamnosus preparation and application thereof
  • Lactobacillus rhamnosus, lactobacillus rhamnosus preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] This example relates to the isolation, pure culture and identification of the Lactobacillus rhamnosus strain of the present invention.

[0030] MRS solid medium with calcium carbonate added: glucose 20.0g; peptone 10.0g; beef extract powder 10.0g; yeast extract powder 5.0g; diammonium hydrogen citrate 2.0g; sodium acetate 5.0g; dipotassium hydrogen phosphate 2.0g; 0.58g of magnesium; 0.25g of manganese sulfate; 1.0mL of Tween 80; 1000mL of distilled water; adjust the pH to 6.2-6.4; add 15.0g of agar;

[0031] Collect saliva and dental plaque samples from healthy oral volunteers, add sterile normal saline to make a 1:10 homogeneous sample solution, shake well, centrifuge at 8000rpm for 5min, collect the precipitate, add an appropriate amount of normal saline to mix the precipitate fully, and The mixed suspension was spread on the MRS solid medium added with calcium carbonate, cultured anaerobically at 37°C for 48 hours, and the typical colonies formed around the calcium-...

Embodiment 2

[0034] This example relates to the functional identification of the Lactobacillus rhamnosus strain of the present invention.

Embodiment 21

[0036] Detection of hydrogen peroxide production ability of strain (Lactobacillus rhamnosus) LR863.

[0037]Detection method: inoculate the strain into MRS medium for static culture at 37°C, centrifuge the fermentation broth at 4°C and 8000rpm for 20min to collect the supernatant, and use a hydrogen peroxide kit to detect the content of hydrogen peroxide in the supernatant.

[0038] Test results: The amount of hydrogen peroxide produced by the strain LR863 after 18h, 24h, and 30h of culture was 0.161g / L, 0.104g / L, and 0.073g / L, respectively, which proved that the strain had the ability to produce hydrogen peroxide.

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Abstract

The invention discloses lactobacillus rhamnosus. A strain (lactobacillus rhamnosus) LR863 is preserved in China General Microbiological Culture Collection Center (CGMCC) and the preservation number isCGMCC No. 14410. The lactobacillus rhamnosus disclosed by the invention has remarkable bacterium-inhibition and sterilization effects on oral pathogenic microorganisms including streptococcus mutans,streptococcus gordonii, porphyromonas gingivalis, fusobacterium nucleatum, actinobacillus actinomycetemcomitans and the like, and has the effects of reducing formation of dental plaque and reducing occurrence and development of oral diseases including tooth decay, periodontitis and the like. A lactobacillus rhamnosus preparation disclosed by the invention can comprise viable bacterium thalli of the lactobacillus rhamnosus, dead bacterium thalli of the lactobacillus rhamnosus, metabolic products of the lactobacillus rhamnosus and intracellular extract, is an oral probiotics preparation which has wide application and a remarkable effect, and can be widely used as an additive of liquid-state foods, solid-state foods, colloidal foods, oral hygiene products and oral treatment drugs.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a Lactobacillus rhamnosus, a Lactobacillus rhamnosus preparation, and an application of the preparation. Background technique [0002] The oral microbiota consists of 700 kinds of microorganisms, including a variety of pathogenic microorganisms. Common clinical oral diseases such as dental caries, periodontitis and halitosis are all related to the excessive growth of pathogenic bacteria or microorganisms that are unfavorable to oral health in the oral cavity . At present, the prevention and treatment of oral diseases mostly start from the perspective of reducing the role of Streptococcus mutans, such as reducing the number of Streptococcus mutans by using mouthwash and reducing sugar intake, or by mechanically removing dental plaque. Probiotics is a general term for a class of active microorganisms that are beneficial to the host. They can produce definite health effects to improve the m...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P1/02A23L33/135C12R1/225
CPCA23L33/135C12N1/20A23V2002/00A61K2035/115C12R2001/225C12N1/205A23V2400/175A23V2200/312A23V2200/30
Inventor 赵林森杨玉红鄢梦洁马新颖齐世华李华文路江浩崔文静李玲
Owner 河北一然生物科技股份有限公司
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