58 results about "Reducing sugar" patented technology

This invention discloses a method for preparing a carbon source for microbial culture medium based on enzymatic hydrolysis of bamboo powder and its application, belonging to the field of high-value utilization technology of biomass resources. Using bamboo powder as raw material, the invention involves mild alkali pretreatment to disrupt the dense structure of lignocellulose, followed by enzymatic hydrolysis using a specialized enzyme system of endoglucanase Cel7B and xylanase XynA expressed heterologously by *E. coli* in an optimized ratio. After solid-liquid separation, an enzymatic hydrolysate rich in reducing sugars is obtained. The hydrolysate is then nutritionally fortified with trace elements such as sodium thiosulfate and sodium silicate to produce a low-cost, high-efficiency liquid carbon source. This invention achieves high-value utilization of bamboo waste. The entire process is mild, environmentally friendly, and simple, easily scaled up industrially, and solves the industry bottleneck of high carbon source cost in industrial microbial culture, demonstrating good application prospects and market value.

This invention relates to the field of fertilizer technology, specifically to an organic liquid fertilizer based on straw enzymatic hydrolysate and its preparation method. The method includes: pretreating corn straw with potassium hydroxide, followed by enzymatic hydrolysis with cellulase and xylanase to obtain the enzymatic supernatant; sequentially treating the supernatant with an ultrafiltration membrane with a molecular weight cutoff of 3000 Da and a nanofiltration membrane with a molecular weight cutoff of 500 Da to collect oligosaccharides with a molecular weight window of 500-3000 Da; partially stabilizing the reducing end of the oligosaccharides with sodium borohydride; pre-complexing with boric acid at pH 8.4-8.8; then sequentially adding relatively low molecular weight sodium polyglutamate, calcium chloride dihydrate aqueous solution, and ultra-high molecular weight sodium polyglutamate to construct a sequential complexation system; subsequently adding potassium nitrate and citric acid aqueous solution; and finally, after filtration at 5 μm and heat treatment at 70℃, obtaining the organic liquid fertilizer. This fertilizer has a high proportion of 500-3000 Da oligosaccharides and a low reducing sugar equivalent, inhibiting storage precipitation and browning, and improving the retention rate of extractable oligosaccharides in the soil.

The application provides an Aspergillus flavus and an enzyme production method and application thereof, and belongs to the technical field of microorganisms.The Aspergillus flavus (Aspergillus flavus) HNZY01 is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.42147.The Aspergillus flavus HNZY01 has the ability of high-yield cellulase, can efficiently and synergistically decompose key structural components such as cellulose, hemicellulose and lignin in tobacco stems, and can also increase the content of reducing sugar in the tobacco stems, so that the Aspergillus flavus HNZY01 can be applied in the degradation of the tobacco stems and the smoking safety of cigarettes can be improved.

This invention provides a low glycemic index blueberry extract and its preparation method, belonging to the field of beverage processing technology. The preparation method includes the following steps: blueberries are pulped, then fermented using a compound strain of Lactobacillus acidophilus, Lactobacillus plantarum, and Bifidobacterium, filtered through a membrane filtration device to obtain a blueberry fermentation broth; blueberries are crushed, the pH of an ethanol solution is adjusted to 2-3 with citric acid, followed by ultrasonic extraction, centrifugation to collect the supernatant, and then vacuum distillation of the supernatant to obtain anthocyanin extract concentrate; the anthocyanin extract concentrate is freeze-dried to obtain anthocyanin extract dried product; the anthocyanin extract dried product and blueberry fermentation broth are mixed evenly at a mass-volume ratio of (0.2-0.3) g:1L, then maltitol is added to adjust acidity and sweetness, homogenized, filtered, and sterilized to obtain the blueberry extract. By using compound probiotic fermentation to reduce sugar content and combining it with alcohol extraction to enhance anthocyanins and improve efficacy, the resulting product has anthocyanin content ≥400 mg / L and a low GI.

The present application relates to a kind of mung bean skin fermentation and its preparation method and application of reinforced anti-oxidation, anti-aging effect, belong to the field of microbial technology.The present application provides a kind of preparation method of mung bean skin fermentation of reinforced anti-oxidation, anti-aging effect, including the following steps: after being mixed with white aspergillus, mung bean skin containing solid-state fermentation medium is carried out aerobic fermentation, after fermentation is ended, it is sterilized and is treated, and the pretreated mung bean skin is obtained;Bifidobacterium is inoculated into the culture medium containing pretreated mung bean skin anaerobic fermentation, and mung bean skin fermentation is obtained.The present application is by mung bean skin as one of the main components of solid-state fermentation medium and white aspergillus aerobic fermentation, so that the cell wall of mung bean skin is degraded, and reducing sugar and polyphenol are released, are used by bifidobacterium, so that the fermentation efficiency of bifidobacterium is improved, and then the content of active ingredient in mung bean skin fermentation is improved, and mung bean skin fermentation is endowed with excellent anti-oxidation, anti-aging effect.

The application discloses a general optimization method for 3D printing sugar-reduced food, comprising the following steps: S1. Defining and measuring core parameters: including porosity P of a 3D printing food matrix, odor concentration C of an odor active component accounting for a total mass proportion of the food matrix, sucrose reduction amount Suc compared with a traditional full-sugar food, and a sensory evaluation index S of a sensory evaluator on a sweetness similarity degree between the sugar-reduced food and the traditional full-sugar food; S2. Constructing a quantitative model: under the condition of setting the sucrose reduction amount Suc value in advance, a plurality of different P, C and S corresponding data are obtained through experimental design, and based on the data, a quantitative model of the sweetness similarity S about the porosity P and the odor concentration C is obtained by using a multiple linear regression method; and the problem that the prior art cannot clearly guide how to set the printing parameters and the formula to obtain the best comprehensive sensory experience under the premise of ensuring a certain sugar reduction ratio is solved.

The application discloses an intelligent fermentation control system for a beer brewing process and relates to the technical field of beer brewing.The system comprises the following steps: crushing malt, saccharifying, filtering, obtaining qualified wort, detecting the concentration, pH value and dissolved oxygen content of the wort, leading the wort into a fermentation tank and sterilizing the wort; detecting the temperature, pH value, carbon dioxide concentration, reducing sugar concentration, alcohol concentration and dissolved oxygen content by using a sensor and standardizing the temperature, pH value, carbon dioxide concentration, reducing sugar concentration, alcohol concentration and dissolved oxygen content; calculating the deviation between each data and the optimal value by using the standardized temperature, standardized pH value and dissolved oxygen content, wherein the optimal value is the medium value of the value allowed during brewing and the minimum value, and adjusting and correcting by using a PID algorithm; calculating a brewing index by using a fermentation environment index and a fermentation rate index, and judging the brewing condition according to the brewing index.

This application relates to a MALDI reactive matrix, 5-methoxy-2-hydroxybenzoyl hydrazine, for the analysis of reducing sugars, belonging to the field of sugar biochemical analysis technology. This application provides the application of 5-methoxy-2-hydroxybenzoyl hydrazine as a MALDI reactive matrix in combination with 2,5-dihydroxybenzoic acid in the analysis of reducing sugars. Specifically, 5-methoxy-2-hydroxybenzoyl hydrazine is the reactive matrix, possessing a hydrazine reactive group that can react with the hemiacetal terminus of reducing sugars. 2,5-dihydroxybenzoic acid acts as a catalyst, significantly promoting the efficient reaction between 5-methoxy-2-hydroxybenzoyl hydrazine and reducing sugars. The 5-methoxy-2-hydroxybenzoyl hydrazine / 2,5-dihydroxybenzoic acid composite matrix provided in this application can form a uniform co-crystallization with reducing sugars, exhibiting good signal reproducibility (RSD < 10%) and good linearity in the range of 0.2~20 pmol / μL (R0). 2 > 0.999).

This invention discloses a Bio-Fenton-driven in-situ polyacrylamide injection fluid depolymerization composition, its preparation method, and its application. The composition comprises an oxidoreductase, reducing sugar, and a transition metal ion source. In use, the composition is added to the injection fluid. The oxidoreductase catalyzes the in-situ oxidation of the reducing sugar to generate hydrogen peroxide, and the transition metal ions from the transition metal ion source mediate a Bio-Fenton reaction, generating highly reactive hydroxyl radicals that attack the polyacrylamide backbone, causing it to break and depolymerize. The final depolymerization viscosity can be reduced to below 5 mPa·s. The composition of this invention works effectively within the temperature range of 45–90°C and pH range of 4.0–10.0. The depolymerization process is stable and controllable. All components and degradation products are non-toxic and biodegradable. All components are in solid form, making storage and transportation safe and convenient.

To provide a liquid cleaning agent composition for hard surfaces that exhibits excellent retention properties and suppression of dispensing failures. [Solution] A liquid cleaning agent composition for hard surfaces containing (A) component: an alkyl polyglucoside represented by the following general formula (A), (B) component: a polyoxyalkylene alkyl ether represented by the following general formula (B), and (C) component: one or more compounds selected from terpene compounds and carboxylic acid esters. a1 -O-[G] a ...(A) [In formula (A), R a1 is an alkyl group with 8 to 20 carbon atoms, G is a residue derived from a reducing sugar, and a is a number between 1 and 5 on average. ], R b1 -O-(AO) b -H...(B) [In formula (B), R b1 is an aliphatic hydrocarbon group with 8 to 18 carbon atoms, AO is an alkylene oxy group, and b is a number representing the average number of repeats of AO, ranging from 31 to 70.

The present application relates to a kind of soybean meal and rapeseed meal mixed fermentation process and its application.Specifically, the present application provides a kind of fermentation raw material, the fermentation raw material includes bran, enzyme and microbial preparation, the bran includes: (1) the soybean meal particle of particle size is 20 mesh or more;(2) the bran particle of particle size is 12 mesh or more except soybean meal;And (3) the zymolyzate of bran particle less than 12 mesh except soybean meal;Wherein, in the fermentation raw material, the mass of (1) and (2) the particle and (3) the mass ratio of zymolyzate is (1.2-2.8):1;In the zymolyzate, the content of reducing sugar is ≥5.0%.The present application can reduce the particle size of soybean meal raw material, increase the addition proportion of protease, improve the content of product small peptide, promote the growth of lactic acid bacteria, and then improve the content of organic acid.

This invention discloses a mesoporous silica-based composite material with phosphotungstic acid simultaneously immobilized with titanium oxide and its cellulose hydrolysis method, belonging to the field of biomass catalytic conversion technology. This invention aims to solve the problems of easy desorption of active components, low catalytic efficiency, and poor cycle stability in existing solid acid catalysts during cellulose hydrolysis. This method uses an ionic liquid as the reaction medium and a titanium-silicon-supported phosphotungstic acid composite material with a two-dimensional hexagonal ordered mesoporous structure prepared by a one-step hydrothermal synthesis as the solid acid catalyst, catalyzing the hydrolysis of cellulose to reduce sugars in the presence of water. This invention achieves highly efficient cellulose conversion through the synergistic catalytic effect of the two acid sites combined with the good solubility of cellulose in the ionic liquid. The catalyst exhibits excellent recyclability, the process conditions are well-defined, it meets the requirements of green chemistry, and it is applicable to the field of biomass resource utilization.

This invention relates to the field of road dust suppression technology, specifically to a bio-based dust suppressant that can be used for road reinforcement. Based on 100% solid content, it contains the following components in the following weight ratio: 100 parts xylose mother liquor and 3-50 parts polyamine crosslinking agent; wherein the xylose mother liquor contains 20%-70% solids by weight, with the remainder being water; wherein the solids contain ≥50% reducing sugars by weight, including D-xylose, L-arabinose, D-glucose, and D-galactose, and the xylose content in the solids is ≥20%. The dust suppressant obtained by this invention uses a large amount of waste xylose mother liquor, a byproduct of the sugar industry. This product is a renewable resource and a low-carbon renewable product. Furthermore, the dust suppressant obtained by this invention not only has dust suppression function but also good compressive strength and water resistance, and can be used as a soil stabilizer. Applying the dust suppressant of this invention to roads can produce a pavement system with excellent overall performance.

The application discloses a kind of flavor beef tallow and preparation method thereof, it is related to food processing technical field, comprising the following steps: step S10, obtain parachlorella protein extract;Step S20, parachlorella protein extract, reducing sugar and beef tallow are mixed, and Maillard reaction is carried out, to obtain first mixture;Step S30, first mixture is heat treated, to obtain second mixture, i.e. the flavor beef tallow.The parachlorella protein extract and reducing sugar are as core, construct dry method Maillard reaction system, generate a variety of rich flavor substances, produce bioactive polypeptide simultaneously, realize beef tallow flavor enhancement and nutrition upgrade.The flavor beef tallow prepared by the application is rich in bioactive polypeptide and Maillard reaction type antioxidant, with rich and pure flavor, balanced nutrition and excellent antioxidant stability.

The present application relates to the technical field of biotechnology, and particularly relates to a chitinase Chi2377 and application thereof in degrading chitin. The amino acid sequence of the chitinase Chi2377 is shown as SEQ ID No. 1. The chitinase Chi2377 can efficiently degrade natural beta-chitin in squid cartilage and other raw materials in cooperation with papain, compared with the use of Chi2377 alone, the produced reducing sugar content can be increased by 5.34 times at most, the enzyme pretreatment step of the raw material is simplified, and the method has the advantages of simple process, green environmental protection, high efficiency and the like, and has important research and application value in the preparation of high-value chitin oligosaccharide and / or monosaccharide and the like by biologically degrading natural beta-chitin.

The application discloses a tobacco source bacillus velezensis chq3-4 and its amylase and application, and belongs to the field of tobacco microorganisms. The bacillus velezensis chq3-4 strain belongs to Bacillus, is classified and named as Bacillus velezensis chq3-4, is preserved in the China Center for Type Culture Collection, the preservation date is June 19, 2024, and the preservation number is CCTCC NO: M 20241305. The amylase crude enzyme solution prepared from the strain has an enzyme activity of 119.28+ / -0.76 U / mL, and the enzyme has strong stability and can still maintain certain enzyme activity under high-temperature and alkaline conditions. The amylase crude enzyme solution is applied to tobacco products, can effectively reduce the content of tobacco starch in the tobacco products, improve the content of reducing sugar, and then improve the quality of the tobacco products.

The present application relates to the technical field of hair dyeing, in particular to a hair dye, application of the hair dye and a hair dyeing method. The hair dye comprises the following components in parts by weight: 10 parts of biological indigo, 0-10 parts of biological melanin, 10-80 parts of reducing sugar, 10 parts of alkaline adjusting agent, 13-23 parts of acidic adjusting agent and 30 parts of water; the mass percentage of indigo in the biological indigo is 40-80%; the mass percentage of melanin in the biological melanin is 90%. The hair dye provided by the present application can dye white hair into blue hair or black hair.

The application discloses a hard carbon negative electrode active material and a preparation method and application thereof. The preparation method of the hard carbon negative electrode active material comprises the following steps: (1) dissolving gelatin and a reducing sugar in water to obtain an electrostatic spinning solution; (2) performing electrostatic spinning on the electrostatic spinning solution by taking an aluminum foil as a receiving base material to obtain an electrostatic spinning nanofiber membrane, and then performing standing to completely volatilize water, so that a gelatin-based nanofiber negative electrode precursor is formed; and (3) performing pre-carbonization treatment on the gelatin-based nanofiber negative electrode precursor in a nitrogen atmosphere, and then performing high-temperature carbonization, so that the hard carbon negative electrode active material is obtained after cooling. The hard carbon negative electrode active material prepared by the method can realize the controllability of the pore structure and the carbon-hydrogen microstructure of the hard carbon negative electrode active material, the prepared hard carbon negative electrode active material does not need a support body and a binder, the weight and the volume of the electrode can be reduced, and the overall energy density and the power density of the battery can be improved.

The application discloses a synthesis method of Amadori compound and belongs to the field of organic synthesis. The application uses lactic acid, glycerol and choline chloride to prepare a low eutectic solvent, which is used as a reaction system of the Amadori compound. The low eutectic solvent system has excellent solubility for reducing sugar and amino acid, does not need a cosolvent, has a uniform reaction system and high mass transfer efficiency. The components (choline chloride, glycerol and lactic acid) used are all natural biodegradable substances, and are non-toxic and non-residual. The subsequent NADES can be recovered by ethanol precipitation, and the recycling rate is more than 95%, and basically no three wastes are discharged.

The invention relates to the technical field of fruit wine brewing, in particular to a method for preparing low-sugar purple sweet potato light wine through multi-strain synergistic primary fermentation, which comprises the following steps: by taking 100% purple sweet potato pulp as a unique fermentation substrate, inoculating a composite strain composed of fruit wine yeast and lactic acid bacteria according to a proper proportion, and fermenting at normal temperature for 7-10 days; and after fermentation, blending the filtrate with xylitol to obtain the low-sugar purple sweet potato light wine with the alcoholic strength of 4-7% vol. Compared with an existing two-stage fermentation technology which contains rice wine and red yeast rice and needs to be inverted, grain auxiliary materials and a post-fermentation procedure are omitted, the fermentation period is shortened by 70%-90%, the sugar source is reduced by 40%, and anthocyanin is enriched; the finished product is clear in color, outstanding in sweet potato fragrance and balanced in sourness and sweetness, and meets the requirements of young consumer groups for low sugar, low alcohol and natural fermentation.

This invention relates to the field of fermentation regulation technology and discloses a multi-parameter intelligent regulation system and method for rapeseed cake fermentation. The method includes: constructing a three-channel synergistic detection system comprising an optical channel, an electrochemical channel, and a conductivity channel; acquiring the original near-infrared absorption spectrum, reducing sugar concentration time series, and conductivity time series of the fermentation broth; quantitatively decoupling the absorbance contribution of Maillard reaction products through the three-channel signals to obtain the true absorbance and content of soluble proteins after removing interference; calculating the net quality yield after Maillard reaction acceleration based on the true improvement rate of soluble proteins and the Maillard reaction degradation rate, thereby determining the optimal fermentation endpoint and outputting a termination signal; this invention eliminates the interference of overlapping peaks of Maillard reaction products in the near-infrared spectral band through multi-dimensional signal coupling and decoupling, realizing the monitoring of the true quality evolution of proteins during fermentation and the identification of the optimal endpoint.

The present application relates to a kind of zanthoxylum bungeanum essential oil emulsion starch enzyme activity detection method, which is by using centrifugal demulsification method combined with accurate chemical analysis method, the starch enzyme activity in zanthoxylum bungeanum essential oil emulsion is quantitatively analyzed, specifically including the following steps: S1, instrument and reagent preparation;S2, solution preparation;S3, sample processing;S4, demulsification treatment and DNS method detects absorbance and calculates starch enzyme activity;The present application can accurately quantify the reducing sugar level in essential oil emulsion, the accuracy compared with direct detection method is significantly improved, and the method is suitable for the starch enzyme activity detection in other essential oil emulsion.

This invention belongs to the field of microbial technology, specifically relating to a starter culture for improving the quality of fermented sweet corn juice and a method for preparing fermented sweet corn juice. The starter culture is a compound of *Lactobacillus plantarum* (CGMCC No. 3151) and *Bifidobacterium longum* subsp. *infantii* (CCTCC NO: M20221253). The starter culture is inoculated into a mixture of saccharified sweet corn juice and hydrolyzed rice protein, fermented for 3-5 hours, and then pasteurized to obtain fermented sweet corn juice. The resulting fermented sweet corn juice exhibits suitable levels of total sugar, pH, total acidity, reducing sugar content, and precipitation rate, significantly increased total phenolic content, strong DPPH free radical scavenging ability, and harmonious flavor, making it suitable for industrial application.

This invention belongs to the field of biotechnology and discloses a glucosylamylase mutant. The amino acid sequence of the glucosylamylase mutant is shown in SEQ ID NO: 1, or an amino acid sequence with more than 90% homology to the above sequence. Compared with the wild type, the glucosylamylase of this invention exhibits significantly improved pH stability, a wider temperature tolerance range, and significantly enhanced heavy metal resistance, making it more suitable for complex industrial environments, especially for hypoglycemic treatments involving heavy metals and large pH fluctuations, such as glutamate mother liquor, and has high industrial application value.

This invention belongs to the field of biomaterials technology, specifically relating to a method for preparing a β-lactoglobulin amyloid cellulose sucrose complex and its application. The complex comprises β-lactoglobulin amyloid cellulose and sucrose, with the sucrose loaded within a three-dimensional network structure formed by the cellulose. The preparation method includes obtaining β-lactoglobulin amyloid cellulose through acid-heat treatment, purifying it, mixing it with sucrose syrup, and then reacting it under heating conditions to obtain the final product. This invention utilizes the high adhesiveness and three-dimensional network structure of β-lactoglobulin amyloid cellulose to prolong the residence time of sucrose on the tongue and slow down its dissolution rate, achieving sustained sweetness release and significantly improving sweetness persistence. The complex has a pure sweetness and no off-flavor, and can be applied to foods such as jelly, chewing gum, and chocolate, achieving the effect of reducing sugar without reducing sweetness, showing promising application prospects.

The present application relates to the technical fields of biological medicine manufacturing, pharmaceutical excipients and preparation manufacturing, and discloses a preparation method of immune-enhanced bovine colostrum powder biological activity protection microcapsule, which is constructed with multi-layer microcapsule structure by multi-component active core, Fc segment protection stable layer, complex coagulation physical barrier layer, double response enteric layer and optional medication time period self-adaptive delay layer, effectively protects heat-sensitive active ingredients such as IgG, lactoferrin and lysozyme in the processes of low-temperature powdering, freeze-drying core making, electrostatic complex coagulation and fluidized bed coating, inhibits Maillard glycosylation reaction in storage period by non-reducing sugar vitrification matrix, free amino acid competitive space agent and activated molecular sieve, maintains FcRn receptor binding activity, reduces shelf life hidden failure, realizes low release in stomach, concentrated release under intestinal conditions, improves stability, targeting, bioavailability and application consistency under different medication time periods.

Non-meat-derived yeast extracts having rich meat flavor, and a preparation method therefor. The method includes: (1) mixing a yeast extract, vitamins, amino acids and water, and adding reducing sugar as needed; (2) adjusting the pH of a mixture obtained in step (1) at 4.0-8.0 with a pH regulator, and stirring the mixture to react at a temperature of 80-130° C. for 30-180 min to obtain a thermal-heated product; (3) adding vegetable oil into the thermal-heated product, and adding edible salt as needed to obtain a material to be dried; and (4) performing vacuum drying on the material to be dried obtained in step (3), and crushing the dried material. According to the present invention, the yeast extract is taken as a main raw material of a Maillard reaction, and a small amount of vitamins, amino acids and reducing sugar are added, so that the formula is simple. The yeast extracts with different rich meat flavor characteristics are obtained via the design of different formulas and process conditions, which better meets the demands of different food fields.

Glycerol-3-phosphate-releasing compounds and pharmaceutical compositions comprising glycerol-3-phosphate-releasing compounds are used in methods of reducing sugar cravings, methods of reducing alcohol cravings, methods of treating diseases mediated by fibroblast growth factor 21, and methods of treating citrin deficiency.