346 results about "Oligopeptide" patented technology

The invention discloses a compound composition with a multi-target heart protection function as well as a preparation method and application of the compound composition. The composition is composed of a basic functional oil phase, a core antioxidant / anti-inflammatory phase, a myocardial nutrition and regulation phase and a bioavailability promotion phase in a synergistic manner, and is integrated into a composite cardiac oligopeptide and microencapsulated particle key component substance through a specific enzymolysis and microencapsulation embedding process system. The invention aims to solve the technical problems of single target spot, insufficient synergistic effect of all components, low bioavailability of active components and poor stability of the existing heart protection product, and provides a stable and easily-absorbed composite composition which can realize synergistic effect from multiple channels at the same time. Technical effect verification shows that under the condition of equal-dose sample feeding, the composition can remarkably improve the bioavailability of functional components and shows excellent myocardial cell oxidative damage resistance and myocardial ischemia improvement activity in cell and animal models, the effect is remarkably superior to that of independent use or simple physical mixing of all the components, and the composition is suitable for clinical application. And the substantial progress of synergistic interaction is proved. The composition can be used for developing various medicines, health foods and functional foods beneficial to heart health.

The invention discloses a preparation method and permeation promotion application of a high-purity sponge bone needle, and belongs to the technical field of sponge bone needles. Comprising the following steps: cutting a natural sponge into pieces, soaking in deionized water, carrying out ultrasonic oscillation, filtering, and cleaning to obtain a pretreated sponge; carrying out enzymolysis on the pretreated sponge with alkaline protease to obtain a primary zymolyte; and carrying out enzymolysis with ginger protease to obtain a secondary enzymolysis product, and carrying out vacuum freeze drying to obtain the high-purity sponge spicule. The natural sponge is subjected to enzymolysis treatment through alkaline protease and ginger protease, and the high-purity sponge spicule is obtained; wheat oligopeptide is grafted on the surface of a sponge spicule, and giant salamander skin collagen, a pomegranate seed extract, a lonicerae flos extract, a centella asiatica extract, an early celery extract, an iris tectorum extract, an oyster shell extract and calcium chloride mixed treatment materials are loaded, so that the repairing effect on skin inflammation acne is improved.

The present invention provides an α-amino acid ester acyltransferase mutant and its application. The α-amino acid ester acyltransferase mutant includes: (a) a protein having the amino acid sequence shown in SEQ ID NO: 1; (b) a protein having an amino acid mutation at at least one of the following sites in the amino acid sequence in (a): P158, K80, T208, A302, A175, N85, N605, K346, S225, I34, or S348, and having the function of an α-amino acid ester acyltransferase; or (c) a protein having more than 80% homology with the amino acid sequence defined in (a) or (b) and having the function of an α-amino acid ester acyltransferase. The α-amino acid ester acyltransferase mutant of the present invention has the advantages of a broad substrate spectrum and high catalytic activity, can be well used in the synthesis of oligopeptides, has low cost and high yield, and realizes true green chemistry.

The invention relates to the technical field of food, in particular to geobacillus stearothermophilus cKM-Geo, application of the geobacillus stearothermophilus cKM-Geo, a shrimp shell oligosaccharide peptide compound and a preparation method of the shrimp shell oligosaccharide peptide compound. The invention provides geobacillus stearothermophilus cKM-Geo, the strain can grow in an oxygen-free environment, ventilation is not needed in the process, and proteolytic enzyme, cellulase, lipase and chitin deacetylase can be highly secreted and expressed; the invention further provides a preparation method for preparing the shrimp shell oligosaccharide peptide by utilizing geobacillus stearothermophilus cKM-Geo fermentation coupling steam explosion treatment, chitin and protein in the shrimp shell are converted into water-soluble oligopeptide and oligosaccharides, the water-soluble oligopeptide and oligosaccharides are separated from calcium carbonate in the shrimp shell, acid and alkali do not need to be used, in the process, waste water is little, pollution is little, and the method is suitable for industrial production. The resource utilization rate is high.

The invention belongs to the technical field of agricultural fertilizers, and particularly relates to a cold-resistant feather amino acid leaf fertilizer and a preparation method thereof.The cold-resistant feather amino acid leaf fertilizer is prepared from, by mass, 86%-95% of feather source amino acid-oligopeptide mixed liquor, 3%-8% of L-lysine, 1%-3% of chelated microelements, 0.5%-1.0% of trehalose, 0.5%-1.2% of polyether modified trisiloxane, 0.5%-1.0% of sodium polyaspartate and 0.1%-0.5% of preservative, wherein the pH (Potential of Hydrogen) value is 5.0 to 6.5; the chelated trace elements are EDTA (Ethylene Diamine Tetraacetic Acid) or amino acid chelates of at least three elements of zinc, iron, manganese, copper and boron. By optimizing a feather pretreatment process and a multi-enzyme synergistic system design and combining functional component compounding, the hydrolysis efficiency and the product activity are remarkably improved, and meanwhile, the permeability and the nutrient transfer effect of the foliar fertilizer under a low-temperature condition are enhanced.

The invention discloses a freeze-dried essence with a repairing effect and a preparation method, and belongs to the field of skin care products, and the freeze-dried essence is composed of freeze-dried powder and an independent solvent liquid. The freeze-dried powder contains 0.5-3 parts of palmitoyl oligopeptide, 3-8 parts of I-type collagen, 1-5 parts of III-type collagen, 0.1-1 part of chitosan, 1-3 parts of secondary variable-temperature fermentation liquor of betula platyphylla and 2-8 parts of a freeze-drying protective agent; the solvent liquid is prepared from 0.5 to 3 parts of hyaluronic acid and 0.05 to 0.5 part of sodium polyglutamate. The preparation method comprises the following steps: a, adding fermentation liquor after dissolving; b, adding nano liposome and a protective agent; c, quickly freezing to-55 + / -2 DEG C by liquid nitrogen, and preserving heat for 2.5-3.5 hours; d, drying for 9-11 hours under the vacuum of 15-25 Pa and at the temperature of-18 + / -2 DEG C; and e, the temperature is increased to 15 + / -2 DEG C in a stepped mode, vacuum is smaller than or equal to 20 Pa, and drying is conducted for 18- Through scientific formula design, multiple effects of efficient repairing, anti-aging and relieving are achieved.

The present invention relates to a compound binding to an endogenous receptor, said compound comprising (i) an oligopeptide comprising a dipeptide with Trp being the C-terminal amino acid of said dipeptide, wherein said Trp is replaced with an α-amino acid Xaa2, whereby the stability in serum or plasma of the peptide bond connecting Xaa2 to the N-terminally adjacent amino acid is increased as compared to the peptide bond connecting Trp to the N-terminally adjacent amino acid in an otherwise identical compound; and (ii) a moiety capable of generating therapeutically effective radiation, said moiety being covalently bound to said oligopeptide.

The invention relates to the technical field of exosomes, and particularly discloses a cosmetic anti-aging mesenchymal stem cell exosome composition, which comprises a therapeutically effective amount of mesenchymal stem cell-derived exosome, the functional carrier comprises a transdermal absorption enhancer and an antioxidant stabilizer; at least one synergistic active component selected from the group consisting of small molecule peptides, plant-derived antioxidants, hyaluronic acid or derivatives thereof; the small molecule peptide is one of oligopeptide-1 and palmitoyl tripeptide-5; the plant source antioxidant is one of resveratrol and ferulic acid; wherein the exosome and the synergistic active component are compounded through a phospholipid bilayer fusion technology; through a phospholipid bilayer fusion technology, the mesenchymal stem cell exosome and a synergistic active component are directionally compounded, an exosome membrane structure is used as a natural carrier, the active component is effectively prevented from being degraded, and meanwhile, the bioavailability is improved by utilizing the targeting property of the exosome membrane structure.

The invention discloses a modular tumor microenvironment response polypeptide and a preparation method and application thereof. The modular tumor microenvironment response polypeptide is composed of the following functional modules: a targeting and chemotactic module used for targeting tumor cell surface p32 protein and tumor lymphatic vessels and promoting tumor tissue penetration and enrichment; the microenvironment double-response module is used for being cut by MMP-2 / 9 enzyme and broken by high-concentration glutathione GSH in a tumor microenvironment to trigger self-assembly; the self-assembly driving module is used for exposing after the microenvironment responds to activation and driving short peptides to be self-assembled to form a nano structure; a killing module: a pro-apoptotic peptide PAD used for targeting a mitochondrial membrane and inducing tumor cell apoptosis; and the stability optimization module is used for enhancing the degradation resistance of the oligopeptide. By integrating targeted delivery, enzyme digestion response, acid-sensitive release and stabilizing structures, the problems of insufficient targeting property, inaccurate release, poor stability, diagnosis-treatment splitting and the like of the anti-tumor drug and the developing agent are solved.

The invention discloses a quaternary synergistic water-soluble fertilizer synergist as well as a preparation method and application thereof, and realizes deep coupling of chemical growth promotion, biological activation and physical slow release, multi-component synergistic interaction and efficient nutrient utilization based on a four-dimensional synergistic mechanism of chitosan oligosaccharide-oligopeptide-bioactive alkali-functional microbial agent. Wherein the modified chitosan oligosaccharide enhances the chelating ability through molecular structure optimization, and forms a nano-scale composite system with the oligopeptide, so that the absorption and transfer efficiency of nutrients on leaf surfaces and root systems is remarkably improved. The functional microbial agent and chitosan oligosaccharide cooperate to activate soil enzyme activity and promote release of insoluble phosphorus and potassium, so that the utilization rate of nitrogen, phosphorus and potassium is remarkably increased compared with that of a traditional fertilizer, and nutrient loss is effectively reduced. The quaternary synergistic water-soluble fertilizer synergist provided by the invention is high in compatibility, can be directly compounded with a commercially available water-soluble fertilizer for use, is suitable for diversified planting scenes of facility agriculture, field crops and commercial crops, and has large-scale popularization potential.

Described herein are compositions comprising an extract from Hibiscus genus and an extract from damaged plant parts from the Moringaceae genus, and methods for stimulating collagen synthesis in skin cells. Also described herein are compositions comprising an extract from Hibiscus genus, an extract from damaged plant parts from the Moringaceae genus, and a complex of actives comprising at least one oligopeptide, at least one extract from the Laminaria genus, and whey protein.

The invention provides a retinyl oligopeptide derivative with anti-aging and wrinkle fading functions and application thereof, and belongs to the technical field of polypeptide derivative application, the retinyl oligopeptide derivative is obtained by connecting a carboxyl terminal of retinoic acid and an amino terminal of oligopeptide, and has a structure as shown in a formula I. In the formula I, n is 1 or 2; l is oligopeptide. The retinoic acid modified oligopeptide derivative provided by the invention has good solubility in both a water phase and an oil phase, is beneficial to product formula design in an application process, has good antioxidant activity and skin cell injury protection activity, can reduce MMP2 and TNF alpha expression increased by ROS in skin fibroblasts, and has a good application prospect. The composition has excellent activity of promoting collagen expression, and has good effects of resisting oxidation, repairing skin injury and smoothing wrinkles when being used for cosmetics or skin care products.

The invention discloses a method for preparing high-F-value oligopeptide from whey protein, and belongs to the technical field of food biology. According to the method, whey protein is taken as a raw material, step-by-step synergistic enzymolysis of alkaline protease-chymotrypsin-flavourzyme is performed, and an activated carbon selective adsorption dearomatization process is combined, so that the F value and the hydrolysis degree of oligopeptide are remarkably improved. The enzymolysis efficiency is high, and the final hydrolysis degree is gt; 30%; the dearomatization effect is excellent, and the product F value is gt; 26; the method is simple in process and low in cost, high-value conversion of whey protein can be achieved, and the prepared high-F-value oligopeptide can be applied to the fields of sports nourishment, food for special medical purposes and the like and has remarkable industrial value.

The invention discloses a composite combined preparation for synergistically treating and conditioning diabetes, relates to the technical field of foods, health foods or dietary supplements for adjuvant therapy and health care of diabetes, and in particular relates to a composite combined preparation for synergistically treating and conditioning diabetes. The preparation comprises albumin peptide, saussurea involucrata culture, vitamin B1, vitamin B2, wheat oligopeptide, corn oligopeptide, collagen peptide, mulberry leaf extract, rhizoma polygonati extract, sea-buckthorn extract, isomaltitol, maltodextrin, ginseng extract and bitter gourd extract. Through the synergistic effect of all the components, comprehensive auxiliary conditioning on diabetes is realized from multiple targets of maintaining colloid osmotic pressure and nutrition, regulating glycolipid metabolism in multiple ways, protecting pancreatic beta cell functions, enhancing insulin sensitivity, preventing nerve and blood vessel complications, meeting special diet requirements and the like. The composition can be prepared into food, health-care food or dietary supplement and other dosage forms.

The invention discloses a preparation method and application of GHK oligopeptide, and belongs to the technical field of biology. According to the method, a nucleotide sequence of the GHK polypeptide is obtained by designing tandem repeat GHK polypeptide ([GHK] 63) and combining preferred codons of bacillus subtilis to optimize a polypeptide coding gene. The GHK oligopeptide is subjected to recombinant expression in bacillus subtilis by utilizing a genetic engineering technology, the obtained recombinant protein is not easy to form an inclusion body and does not contain endotoxin, and the high-yield GHK oligopeptide is obtained after separation, purification and protease digestion treatment. According to the GHK oligopeptide preparation method provided by the invention, extra optimization treatment does not need to be carried out on tandem repeat GHK polypeptides, the yield of the GHK oligopeptide is improved, the whole production process is simplified, the cost is low, and the method is suitable for large-scale production of blue copper peptides and has wide application prospects in the fields of medicines, medical beauty and the like.

The invention discloses a plant extract-based transdermal repair composition and a preparation method thereof, and belongs to the technical field of biology, the plant extract-based transdermal repair composition comprises glycerin, an emulsifier, sodium hyaluronate, oligopeptide-1, a plant extract eutectic compound and a proper amount of deionized water, and the glycerin, the emulsifier, the sodium hyaluronate, the oligopeptide-1 and the plant extract eutectic compound are uniformly mixed to form an ointment. According to the application, the extraction method of the polygonum cuspidatum root and the peony root bark is optimized, L-proline is added into an extraction solution to enhance the molecular polarity, glycine promotes crystallization to form nanoscale crystals, resveratrol in the polygonum cuspidatum root and paeonol in the peony root bark form a stable compound through a supramolecular eutectic technology, and the resveratrol-paeonol compound is prepared. The problems of poor light sensitivity and heat sensitivity of resveratrol and poor water solubility of paeonol are solved, and the eutectic structure prolongs the half-life period of the active component and improves the transdermal effect, thereby facilitating the effective component to play corresponding functions and improving the repair effect.

The invention discloses camellia seed meal oligopeptide as well as a preparation method and application thereof. The method comprises the following steps: S1, pretreating camellia seed meal to obtain a pretreated substance; s2, performing enzymolysis and wall breaking to obtain first enzymatic hydrolysate; wherein the enzymolysis wall breaking adopts a mixed enzyme, and the mixed enzyme comprises amylase and cellulase; s3, extracting crude protein to obtain crude protein; s4, carrying out crude protein enzymolysis on the crude protein to obtain a second enzymatic hydrolysate; wherein the crude proteolysis adopts mixed protease, and the mixed protease comprises first protease and second protease; wherein the first protease is one or more of alkaline protease, neutral protease and acid protease; the second protease is papain; and S5, purifying the second enzymatic hydrolysate to obtain the camellia seed meal oligopeptide. The average molecular weight of the obtained product is smaller than 1000 Daltons, and the product has biological activity and high application value in the fields of cosmetics and functional food.

The invention relates to repairing essence and a preparation method thereof, and belongs to the technical field of cosmetics. The repairing essence disclosed by the invention contains a repairing composition, wherein the repairing composition is prepared from oligopeptide-215, Ectoine, a codium tomentosum extract, a liquorice root extract and a purple coneflower extract; the purple coneflower extract is prepared by taking purple coneflower as a raw material, performing mixed fermentation through bacillus amyloliquefaciens and lactobacillus plantarum and then performing ultrasonic extraction, and the obtained purple coneflower extract has an improved anti-inflammatory effect. The five components in the repair composition can synergistically promote expression of skin barrier repair related proteins, and the repair essence containing the repair composition can significantly improve the skin TEWL value and the skin moisture content of a subject and improve the redness condition, and has significant moisturizing, soothing and barrier repair capabilities.

This invention relates to the field of cosmetics, and more particularly to active oligopeptides and their application in the preparation of anti-aging products. This invention provides an active oligopeptide with anti-aging activity, the amino acid sequence of which is shown in SEQ ID NO:1 and / or SEQ ID NO:4. This invention also provides an active oligopeptide (β1 and β1c) designed based on computational structural biology, targeting the β-Klotho FGF19 / / FGF21 and receptor-binding arm (RBA) region, and optimized for physicochemical properties, for use in skin anti-aging.

The application discloses a kind of earthworm protein with twelve 3 peptides, which is prepared by degrading earthworm protein with catalytic amount of Pro-Ala-Lys. The application also discloses the preparation method of the earthworm protein with twelve 3 peptides and the application of the earthworm protein with twelve 3 peptides in removing ·OH free radicals and DPPH free radicals. Experimental results show that the earthworm protein with twelve 3 peptides has excellent effects in removing ·OH free radicals and DPPH free radicals. Therefore, the earthworm protein with twelve 3 peptides provides an effective technical means for preparing ·OH free radical scavenger and DPPH free radical scavenger.

The invention provides an external composition for improving the sub-health state of people in a high-glucose lifestyle and application of the external composition, and belongs to the technical field of biological agents. The composition provided by the invention is prepared from the following components: caffeine silanol C, a radix sophorae flavescentis extract, a lotus leaf extract, a corn poppy extract, a bitter gourd extract, soy isoflavone, beta-glucan, a tea leaf extract, an apple extract, resveratrol, a pueraria mirifica extract, a propolis extract, an angelica sinensis root extract, a radix ophiopogonis extract and a rhizoma anemarrhenae root extract. The composition is prepared from a fructus gardeniae extract, a Thermus thermophilus fermentation product, oligopeptide-1 and carnosine. The composition provided by the invention can be prepared into an external preparation, is suitable for people in the high-glucose lifestyle, has the technical effects of improving the sub-health states of the people in the high-glucose lifestyle, such as weakness, skin itch, dark skin color, thirst, frequent urination and the like, and improves the life quality of the people in the high-glucose lifestyle.

The present invention relates to a cosmetic composition comprising galactomyces, having skin-soothing, moisture-retaining, skin tone-enhancing, and skin barrier-improving properties, and more specifically, comprises a galatomyces fermentation filtrate, a rice lees distillate, sh-oligopeptide-1, a plant extract, a skin conditioning agent, a pH regulator, a surfactant, an emollient, a sequestering agent, a film-forming agent, and a thickener. The cosmetic composition comprising said ingredients is less likely to cause skin irritation by having a large amount of natural ingredients, is eco-friendly by upcycling rice lees, exhibits skin-soothing, moisture-retaining, skin tone-enhancing, and skin barrier-improving effects by containing an abundant amount of galactomyces, vitamins, minerals, and organic acid ingredients, and improves skin health by preventing skin aging and skin damage and promoting skin cell regeneration.

This application discloses a seaweed oligopeptide fertilizer for promoting root growth and seedling development, and its production process, belonging to the field of fertilizer technology. The fertilizer, by weight, comprises: 2-10 parts seaweed oligopeptide complex, 1-8 parts seaweed extract, 10-30 parts compound amino acids, 1-10 parts blueberry pomace, 20-50 parts oxytetracycline waste decomposition products, and 30-60 parts recycled organic matter from kitchen waste. By combining seaweed oligopeptides with chitosan to form a complex, the stability of the active ingredients is improved; simultaneously, the synergistic utilization of oxytetracycline fermentation waste, kitchen waste, and blueberry pomace achieves resource utilization. Experiments show that this fertilizer significantly promotes crop root growth and seedling development.

The invention belongs to the technical field of cosmetics, and discloses an avocado fermentation composition as well as a preparation method and application thereof. The composition comprises 1%-10% of avocado fermentation filtrate, 0.1%-2% of alpha-glucan oligosaccharide, 0.1%-2% of a wild chrysanthemum flower extract, 3%-8% of a humectant, 0.01%-0.1% of a chelating agent and the balance of deionized water. Wherein the avocado fermentation filtrate is prepared by compound fermentation of aphid torulopsis and candida and is rich in oligopeptide, free amino acid, lactic acid and polyphenol. According to the invention, the filtrate obtained by fermenting avocado with a specific composite strain is scientifically compounded with prebiotics with definite flora selectivity to construct a multifunctional composition with definite structure, synergistic function and high stability; the composition not only provides direct barrier repairing and moisturizing effects, but also actively guides skin micro-ecology to succession to a healthy steady state through nutrition intervention, so that the skin health level is synchronously improved in the two dimensions of molecules and ecology.

The invention relates to the technical field of daily chemicals, and provides an oat amino acid surfactant and a preparation method thereof. The preparation method comprises the following steps: dissolving oat protein polypeptide in deionized water, heating to 50-70 DEG C, adjusting the pH value of a system to 4-10, adding proteolytic enzyme, stirring for 4-6 hours, maintaining the pH value of the system to 4-10, cooling to room temperature, and drying to obtain the oat oligopeptide. The preparation method comprises the following steps: dissolving a sodium hydroxide solution into deionized water, controlling the temperature to be 50-70 DEG C, adjusting the pH value of a system to be 11-12, dropwise adding fatty acyl chloride, keeping the pH value of the system to be 11-12, continuously keeping the pH value of the system to be 11-12 after dropwise adding, and reacting for 0.5-1.5 hours; performing curing; and cooling to 40-60 DEG C, decolorizing, and adjusting the pH value of the system to 6.5-7.5 to obtain the oat amino acid surfactant. The preparation method is simple in process, and the obtained oat amino acid surfactant is light in chromaticity and low in smell.

The invention belongs to the technical field of protein peptide preparation, and particularly relates to wheat oligopeptide with an anti-fatigue effect and a preparation method of the wheat oligopeptide. According to the method, the raw materials are subjected to enzymolysis by using the first compound enzyme containing bacillus subtilis protease and bromelain and the second compound enzyme containing trypsin, flavourzyme and papain step by step, and treatment of centrifugation, ultrafiltration and the like is combined, so that the yield and the taste of the product are improved, and the proportion of small molecular weight peptide in the product is high. Therefore, the wheat oligopeptide prepared by the method is high in yield and good in taste, not only is beneficial to improving the endurance of the body and prolonging the exercise time, but also can improve the energy metabolism condition in the body, delay the accumulation of lactic acid, urea nitrogen and creatine kinase in the body after exercise and increase the glycogen storage amount, and macroscopically shows certain anti-fatigue ability. The technical scheme of the invention has strong practicability, is beneficial to industrial production, and can be applied to preparation of anti-fatigue functional food.

An oligopeptide has the amino acid sequence of Thr-Val-Asp-Ser-Cys-Leu-Thr (SEQ ID NO: 1) with addition or insertion of a (poly)peptide to the oligopeptide, or substitution of a (poly)peptide for a part of amino acids of the oligopeptide.

The present invention relates to peptide conjugates that comprise a plant-derived oligopeptide capable of crossing the blood-brain barrier (BBB) and a therapeutic or diagnostic agent. The BBB-penetrating oligopeptide is an oligopeptide according to Formula I, Asp–R2–Gly–Leu–R5–R6–R7–Leu–Gly–R10–R11–R12, wherein R2 represents Arg, Lys, Cyt, D-Arg or Orn; R5 represents Phe, Arg, Lys, His, Trp, Tyr, D-Tyr, D-Phe, Orn, 4-aminophenylalanine or 3-phenylpropionate; R6 represents Pro, Leu, Glu or Lys; R7 represents Phe or Trp; R10, R11 and R12 each independently represent Lys, Arg or Orn; or an oligopeptide according to Formula II, Glu–R2'–R3'–Gly–R5'–R6'–Glu–R8'–R9'–R10'–Glu–R12'–Leu–Pro–Gly, wherein R2', R3', R8', R10' and R12' each independently represent Lys, Arg or Orn; R5' represents Phe, Ile, Arg, Lys, His, Trp, Tyr, D-Tyr, D-Phe, 4-aminophenylalanine, or 3-phenylpropionate; R6' represents Met, Leu, Ile, Asn, norleucin, D-norleucin, seleno- methionine or D / L-2-hydroxy-(4-methylseleno)butanoic acid; and R9' represents Leu or Ile. The conjugates comprising a BBB-penetrating oligopeptide compound and a therapeutic agent can be used in treatments for diseases of the central nervous system (CNS). Furthermore, conjugates with a BBB-penetrating oligopeptide compound and a diagnostic agent can be used in both in vivo and in vitro diagnostic methods.

The invention discloses a sebum-imitated recombinant collagen anti-oxidation anti-aging brightening composition and a preparation method thereof, the sebum-imitated recombinant collagen anti-oxidation anti-aging brightening composition is of a core-shell double-phase structure, and the sebum-imitated recombinant collagen anti-oxidation anti-aging brightening composition comprises an aqueous phase core, an aqueous phase core and a composite material, the active ingredient of the aqueous phase core is a covalent bond of carboxymethylated recombinant human collagen III and eclipta oligopeptide; the oil-phase layer wraps the water-phase core and comprises a sebum-imitating three-phase film as well as an antioxidant active matter and a slow-release carrier which are dispersed in the sebum-imitating three-phase film; wherein the volume ratio of the water-phase core to the oil-phase layer is 1: 2-1: 4, a core-shell double-phase structure with the carboxymethylated recombinant collagen-eclipta oligopeptide covalent bond as the water-phase core and the sebum-imitating three-phase membrane functional carrier as the oil-phase layer is constructed, and the limitation that active ingredients in a traditional formula are simply mixed is broken through. According to the structure, the stability of the water phase core is ensured through a chemical covalent bond, and the percutaneous permeability and biocompatibility of the composition are remarkably improved by utilizing the high similarity of a sebum-imitating film and skin lipid.

The invention belongs to the crossing field of marine biotechnology and bioinformatics, and particularly relates to a method for high-throughput identification of natural functional oligopeptides. At present, bottlenecks still exist in development and application of various functional oligopeptides such as penetrating peptides and antibacterial peptides in non-model species, and the high adaptability of the existing functional oligopeptides in complex and diverse non-model organisms is limited mainly due to differences (amino acid preference and receptor specificity) among species. Therefore, the invention provides a method for high-throughput identification of functional oligopeptides from natural sequences of organisms by means of machine learning. According to the method, based on a biological natural protein sequence, an oligopeptide sequence set meeting a preset length condition is generated through a sliding window strategy system, a deep machine learning model is utilized to perform high-throughput functional prediction on the oligopeptide sequence, and on the basis of performing'interruption-prediction-re-comparison 'on existing functional oligopeptides, the functional oligopeptide sequence set meeting the preset length condition is obtained. The comparison threshold value for accurately identifying the functional oligopeptide can be determined, and the natural functional oligopeptide existing in the protein sequence can be effectively identified based on the threshold value. The functional oligopeptide obtained by the method has strict screening of physicochemical properties of a machine learning model and high-adaptability biological characteristics formed in species evolution. According to the method, the direct application capability of the functional oligopeptide in non-model species is remarkably improved, and an accurate and high-throughput technical means is provided for efficient development and application of the functional oligopeptide in non-model organisms.