692 results about "Oligopeptide" patented technology

The invention relates to an anti-aging oligopeptide for beauty and skin care, the oligopeptide has an amino acid sequence as shown in (X1) (R or K or H) G (D or E) # (X2), X1 represents one amino acid of G, A, V, L, I and P or represents that no amino acid exists, # represents one amino acid selected from A, V, D, E, N and Q, and X2 represents G and P or represents that no amino acid exists. The oligopeptide is a functional fragment of XVII type collagen, can induce in-vivo collagen synthesis or simulate a protein regulation function, has the effects of promoting cell self-cleaning, helping to remove aged skin cells, reconstructing skin rhythm, maintaining a good skin structure, resisting initial aging, promoting skin repair and regeneration, reducing fine wrinkles and the like, and is mild in action, free of side effects and free of toxic and side effects. The product has no obvious adverse reaction on human body, and is a good beautifying and anti-aging product.

The present disclosure provides a preparation method of a yak hide-derived oligopeptide ferrous chelate with a high antioxidant activity. The preparation method includes preparing a yak hide-derived oligomeric collagen peptide and subjecting the yak hide-derived oligomeric collagen peptide as a protein source to chelation with an iron source in water. A pretreated yak hide is subjected to enzymatic hydrolysis under a pH value of 7 at 50° C. for 4 h with an amount of an enzyme added at 2% to obtain a yak skin-derived collagen with a molecular weight of less than 2 kDa; the chelation is conducted in a peptide-to-iron mass ratio of 1:1 to 5:1 with a peptide concentration of 1% to 5% at 30° C. to 70° C. for 20 min to 60 min under a pH value of 3 to 8; and an iron chelating capacity is 42.72 mg / g under optimal preparation conditions.

The invention discloses an oligopeptide composition for inhibiting inclusion body formation and promoting Gag-Like protein nanoparticle self-assembly, the oligopeptide composition comprises a C-terminal oligopeptide and an N-terminal oligopeptide, the amino acid sequence of the N-terminal oligopeptide is as shown in SEQ ID NO: 1, and the amino acid sequence of the C-terminal oligopeptide is as shown in SEQ ID NO: 2. The oligopeptide composition can effectively avoid formation of a prokaryotic expression protein inclusion body and / or promote dissolution of prokaryotic expression protein, the oligopeptide composition is further utilized to be combined with HIV-1p24 and human endogenous retrovirus K capsid protein CA, nanoparticles are prepared respectively, and the oligopeptide composition can be used as a carrier for antigen surface display or drug transportation. The nanoparticles formed by the HIV-1 capsid protein p24 and the human HERV K CA have cavities which are obviously larger than those of ferritin, so that the nanoparticles have higher loading capacity; as an antigen display platform, the antibody also has good stability and wide applicability.

A method for visible light-mediated chemical modification of a polypeptide or protein based on cysteine includes the following steps: allowing a cysteine residue on a cysteine-containing oligopeptide, polypeptide, or protein to undergo a reaction with an activating reagent to generate a free radical precursor in situ; and subjecting the free radical precursor to desulfurization under photocatalytic condition, and allowing the generated free radical intermediate to undergo an addition reaction with an olefin or an alkyne, such that sulfhydryl removal and carbon-carbon bond construction are achieved to produce a chemical modification product of the polypeptide and protein. The method cleverly avoids the influence on the chiral center, and thus the chirality of the amino acid residue can be retained. The method is conducive to well avoiding the occurrence of side reactions, and thus the efficiency of the entire conversion is very high.

The invention discloses a compound composition with a multi-target heart protection function as well as a preparation method and application of the compound composition. The composition is composed of a basic functional oil phase, a core antioxidant / anti-inflammatory phase, a myocardial nutrition and regulation phase and a bioavailability promotion phase in a synergistic manner, and is integrated into a composite cardiac oligopeptide and microencapsulated particle key component substance through a specific enzymolysis and microencapsulation embedding process system. The invention aims to solve the technical problems of single target spot, insufficient synergistic effect of all components, low bioavailability of active components and poor stability of the existing heart protection product, and provides a stable and easily-absorbed composite composition which can realize synergistic effect from multiple channels at the same time. Technical effect verification shows that under the condition of equal-dose sample feeding, the composition can remarkably improve the bioavailability of functional components and shows excellent myocardial cell oxidative damage resistance and myocardial ischemia improvement activity in cell and animal models, the effect is remarkably superior to that of independent use or simple physical mixing of all the components, and the composition is suitable for clinical application. And the substantial progress of synergistic interaction is proved. The composition can be used for developing various medicines, health foods and functional foods beneficial to heart health.

The invention discloses a preparation method and permeation promotion application of a high-purity sponge bone needle, and belongs to the technical field of sponge bone needles. Comprising the following steps: cutting a natural sponge into pieces, soaking in deionized water, carrying out ultrasonic oscillation, filtering, and cleaning to obtain a pretreated sponge; carrying out enzymolysis on the pretreated sponge with alkaline protease to obtain a primary zymolyte; and carrying out enzymolysis with ginger protease to obtain a secondary enzymolysis product, and carrying out vacuum freeze drying to obtain the high-purity sponge spicule. The natural sponge is subjected to enzymolysis treatment through alkaline protease and ginger protease, and the high-purity sponge spicule is obtained; wheat oligopeptide is grafted on the surface of a sponge spicule, and giant salamander skin collagen, a pomegranate seed extract, a lonicerae flos extract, a centella asiatica extract, an early celery extract, an iris tectorum extract, an oyster shell extract and calcium chloride mixed treatment materials are loaded, so that the repairing effect on skin inflammation acne is improved.

The invention discloses collagen polypeptide firming and anti-wrinkle freeze-dried powder, and relates to the technical field of cosmetics. The invention relates to a biological preservative which is prepared from water, butanediol, a bacillus / soybean fermentation product extract, soluble collagen, acetyl hexapeptide-8, panthenol, 1, 2, 4-trimethyl-1, 3-pentanediol and a proper amount of water. The cosmetic is prepared from 1, 2-hexanediol, tetrahydromethylpyrimidine carboxylic acid, hydroxypropyl tetrahydropyrantriol, tremella polysaccharide, saccharide isomeride, a bifidus yeast fermentation product lysate, sodium hyaluronate, DNA sodium, a radix gentianae extract, mannitol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, oligopeptide-1, decapeptide-4, oligopeptide-3, hydrolyzed royal jelly protein, folic acid and disodium hydrogen phosphate. The additive is composed of sodium dihydrogen phosphate, citric acid and sodium citrate. The mask can provide rich nutrients for skin, deeply care the skin, moisturize and relieve the skin, tighten the skin, resist wrinkles and make the skin moisturized, glossy, elastic and tight, and is high in use safety and wide in application prospect.

The invention discloses a wheat peptide with a hypoglycemic effect as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The invention provides a bioactive peptide LPQ or LPQF with a hypoglycemic effect, and animal model experiments prove that the bioactive peptide can effectively reduce the blood sugar level and improve related metabolic indexes, and has no obvious side effects. The invention also provides a wheat peptide rich in oligopeptide containing the peptide fragment LPQ, and animal model experiments prove that the wheat peptide has a remarkable effect in the aspect of reducing blood sugar. The invention provides a safe and effective alternative scheme for development of diabetes treatment drugs, and has good market prospects and application potential.

The invention relates to an external composition for hair growth regulation and a preparation method thereof, and belongs to the technical field of cosmetics and medicines. The composition disclosed by the invention comprises nano mesoporous carbon for enhancing permeation, various surface functional modifications on the nano mesoporous carbon, minoxidil, protein fragments, polypeptides, oligopeptides, amino acids, dutasteride, bimatoprost, traditional Chinese medicine extracts, vitamins, caffeine, nucleosides, growth factors and various auxiliary functional components. Wherein the pore diameter of mesopores of the nano mesoporous carbon for enhancing permeation is 2-30 nm, the total pore volume of the mesopores is 1.5-3.5 cm < 3 > / g, and the particle size of the nano mesoporous carbon is 50-200 nm. The prepared nano mesoporous carbon is granular, controllable in size, stable in structure, free of irritation to skin, capable of keeping inert to various active ingredients in the composition, capable of enhancing absorption and permeation of other ingredients on the skin, wide in application range, capable of compounding various types of active ingredients and high in biological safety.

The invention discloses a self-assembly enzyme for directional synthesis of uniform low molecular weight dextran, the self-assembly enzyme is named as YG-SpyTag-SpyCatcher-padex, the YG is a dextran sucrase gene (Genbank No.DQ345760), the padex is a dextran enzyme gene (Genbank No.KF999646.1), the SpyTag and the SpyCatcher are two specific oligopeptides, and aspartic acid in the SpyTag and lysine in the SpyCatcher spontaneously form an isopeptide bond. The invention belongs to the technical field of biology, and relates to an enzyme engineering technology. According to the invention, dextran sucrase derived from an escherichia coli genetically engineered bacterium BL21 (DE3)-YG-SpyTag and dextran enzyme derived from a pichia pastoris genetically engineered bacterium X33-SpyCatcher-padex are combined through a SpyTag-SpyCatcher protein covalent coupling system, and the obtained self-assembled enzyme can be used for catalyzing the substrate sucrose to be directly converted into dextran with the molecular weight of 3000 to 4000 Da.

The invention relates to the technical field of biological medicines, in particular to a recombinant rabies virus nano-antibody fused with a solubilizing oligopeptide and a cell penetrating peptide and application of the recombinant rabies virus nano-antibody in preparation of rabies prevention and treatment products. The recombinant rabies virus nano-antibody fused with the solubilizing oligopeptide and the cell penetrating peptide comprises a humanized rabies virus nano-antibody, and the C end of the humanized rabies virus nano-antibody is fused with a solubilizing tag peptide P17 and the cell penetrating peptide. The recombinant rabies virus nano antibody utilizes a prokaryotic expression system, is low in cost, has excellent cell membrane and BBB penetrating ability, can enter a central nervous system from veins for virus neutralization treatment, and has an efficient passive immune effect.

The invention discloses application of oligopeptide in preparation of a medicine for treating and / or preventing diabetic nephropathy. The oligopeptides comprise oligopeptides with the amino acid sequence of SEQ ID NO: 1: LNLYP and derivative sequences or conservative substitution variants of the oligopeptides. The oligopeptide can significantly improve renal function indexes of mice with diabetic nephropathy, such as UACR, serum creatinine, urea nitrogen and the like, enhance podocyte barrier integrity and inhibit accumulation of advanced glycosylation end products AGEs in kidney tissues, and provides a basis for development of novel drugs for treating diabetic nephropathy.

The invention relates to a biosynthesis method of cosmetic tripeptide GHK (glycine-histidine-lysine), which is characterized in that a GHK oligopeptide is prepared by carrying out microbial fermentation synthesis and trypsin specific hydrolysis on artificially designed GHK multiple artificial sequences. And on the basis of the original sequence, the GHK yield is improved by adding a specific interval sequence. Compared with a traditional chemical synthesis method, the GHK oligopeptide biosynthesis method has the advantages that raw materials are cheap and simple, steps are easy to implement, and the GHK oligopeptide biosynthesis method is environmentally friendly and beneficial to improving the application level of GHK tripeptide in the field of beauty makeup.

The present invention provides an α-amino acid ester acyltransferase mutant and its application. The α-amino acid ester acyltransferase mutant includes: (a) a protein having the amino acid sequence shown in SEQ ID NO: 1; (b) a protein having an amino acid mutation at at least one of the following sites in the amino acid sequence in (a): P158, K80, T208, A302, A175, N85, N605, K346, S225, I34, or S348, and having the function of an α-amino acid ester acyltransferase; or (c) a protein having more than 80% homology with the amino acid sequence defined in (a) or (b) and having the function of an α-amino acid ester acyltransferase. The α-amino acid ester acyltransferase mutant of the present invention has the advantages of a broad substrate spectrum and high catalytic activity, can be well used in the synthesis of oligopeptides, has low cost and high yield, and realizes true green chemistry.

The invention discloses a calcium-containing antarctic krill oligopeptide nutritional composition for promoting bone cell growth and application thereof, and belongs to the technical field of functional foods.The calcium-containing antarctic krill oligopeptide nutritional composition is composed of antarctic krill oligopeptide and a calcium compound, and the mass ratio of the antarctic krill oligopeptide to the calcium compound is (0.5-5): 1; or the composition is composed of the euphausia superba oligopeptide, the calcium compound and the vitamin D, and the mass ratio of the euphausia superba oligopeptide to the calcium compound to the vitamin D is (0.5-5): 1: (2.5 * 10 <-5 >)-(15 * 10 <-5 >); the mass of the calcium compound is calculated according to the content of the calcium element in the antarctic krill oligopeptide; the antarctic krill oligopeptide is obtained by treating antarctic krill, antarctic krill powder or defatted antarctic krill powder by utilizing an enzymolysis method; the content of a peptide fragment with the molecular weight of less than 1000Da in the antarctic krill The raw materials of the nutritional composition are easy to obtain, the safety is high, and the nutritional composition can be used for preparing products with the effect of promoting bone cell growth.

The invention relates to a preparation method of small-molecular-weight sea cucumber oligopeptide, the prepared sea cucumber oligopeptide and application, and belongs to the technical field of skin care products. The preparation method of the small-molecular-weight sea cucumber oligopeptide comprises the following steps: preparing sea cucumber fine powder; adding the sea cucumber fine powder into ultrapure water, adding papain and EDTA (Ethylene Diamine Tetraacetic Acid), and carrying out enzymolysis under the assistance of microwaves; adding trypsin and calcium ions, and carrying out enzymolysis under the assistance of microwaves; adding neutral protease and zinc ions, and performing enzymolysis under the assistance of microwaves to obtain final sea cucumber enzymatic hydrolysate; and decolorizing, separating and purifying, and freeze-drying to obtain the powdery small-molecular-weight sea cucumber oligopeptide. According to the preparation method of the small-molecular-weight sea cucumber oligopeptide, microwave heating and enzymolysis reaction are combined, the hydrolysis degree of a peptide chain is regulated and controlled by controlling an enzyme hydrolysis sequence and enzymolysis conditions, the small-molecular-weight sea cucumber oligopeptide with relatively concentrated and moderate molecular weight is prepared, and good biological activity of the sea cucumber oligopeptide is kept.

The invention belongs to the technical field of preparation of sea cucumber peptides, relates to preparation of sea cucumber small molecule peptides, and relates to a preparation method of sea cucumber peptides with composite effects of protecting liver and preventing liver cirrhosis, and the sea cucumber peptides with various effects are prepared by using fresh and alive sea cucumbers as raw materials through protease hydrolysis, fishy smell removal, deodorization and other processes. The sea cucumber peptide has the advantages of good solubility, good stability, low viscosity and the like. Compared with the transportation function of amino acid, the sea cucumber active oligopeptide has the characteristics of quick absorption, low consumption and difficulty in saturation, can be added into food, health food, medicine and the like, and has the effects of supplementing nutrition, protecting the liver function, preventing liver cirrhosis, supplementing mineral substances, protein and vitamins and the like.

The invention belongs to the technical field of biological medicine, and relates to a composite polypeptide composition as well as a preparation method and application thereof. According to the technical scheme, pH responsive polymer loaded oligopeptide-1, acetyl heptapeptide-4 and palmitoyl tripeptide-1 are adopted as a core, hyaluronic acid is adopted for wrapping, a hyaluronic acid layer adsorbs palmitoyl tripeptide-8, heptapeptide-6 and decapeptide-4, and the composite polypeptide with a core-shell structure is prepared and used for treating inflammatory skin diseases. In the acute stage of inflammation, the active ingredients on the outer layer quickly inhibit the activation of proinflammatory factors and immune cells and relieve red, swollen and heat pain, and the gel outer layer formed by hyaluronic acid realizes continuous release, prolongs the action time and supplements moisture to the skin; the pH-responsive polymer of the inner layer releases active ingredients at the inflammation part, so that relapse is prevented, and the skin barrier function is repaired. The composition plays a role from multiple dimensions of inflammation regulation, barrier repair, oxidation resistance and the like through a multi-layer structure design and a multi-component synergistic effect.

The invention provides lucid ganoderma oligopeptide with an anti-tumor effect and a preparation method thereof. The three oligopeptides are separated and purified from the ganoderma lucidum for the first time, and the oligopeptides have broad-spectrum anti-tumor activity, can effectively inhibit tumor cell proliferation, have good biological compatibility and have great application prospects.

The invention discloses an essence composition containing lipidosome with an acne removing effect as well as a preparation method and application of the essence composition. The essence composition comprises the following components: 2-30% of lipidosome with the acne removing effect, 0.5-15% of a skin conditioner, 5-40% of a humectant, 0.1-3% of a preservative, 0.15-10% of a thickening agent, 0.05-5% of a chelating agent, 0.01-3% of a pH regulator and deionized water. The lipidosome with the acne removing effect is prepared from the following components in percentage by weight: 1.5 to 2.5 percent of white willow bark extract, 0.3 to 0.7 percent of Pinosine, 2 to 4 percent of rhizoma bletillae root extract, 4 to 6 percent of nicotinamide, 0.15 to 0.35 percent of oligopeptide-3, 7 to 12 percent of lecithin and 1 to 2 percent of cholesterol. In the preparation process, the lipidosome with the acne removal effect is reasonably compounded with other auxiliary material matrixes, so that efficient transmission and absorption of active ingredients can be promoted, irritation to skin can be effectively relieved, meanwhile, the overall effect of the essence is enhanced, the essence has mildness and high efficiency, and the high-efficiency requirement of consumers for the acne removal skin care product is met.

The present application relates to the field of cosmetic technology, more specifically, it relates to an anti-aging composition, anti-aging cosmetics and anti-aging essence.An anti-aging composition is composed of hydroxypinacolone retinoic acid ester, Buddleja flos extract and oligopeptides, and the weight ratio of the hydroxypinacolone retinoic acid ester, Buddleja flos extract and oligopeptides is 1:(0.20-0.25):(6.5-7.0).The anti-aging composition of the present application, using hydroxypinacolone retinoic acid ester, Buddleja flos extract and oligopeptides to be compounded, can effectively resist ultraviolet light, has good antioxidant and hydrating properties, can be applied to prepare cosmetics, improve skin wrinkles.

According to the chlorella pyrenoidosa high-F-value peptide and the preparation method thereof, parameters in the enzymolysis and adsorption processes are strictly controlled, and the enzymolysis of the incision enzyme and the excision enzyme not only can efficiently hydrolyze chlorella protein and generate more peptide fragments with biological activity, but also can be used for preparing the chlorella pyrenoidosa high-F-value peptide. And the characteristics of high industrial production cost and poor stability caused by wide enzyme cutting sites are solved. The prepared chlorella pyrenoidosa high-F-value peptide reaches 36.90 + / -8.18, the F value of the chlorella pyrenoidosa high-F-value peptide is increased by 12.72 times compared with the chlorella pyrenoidosa raw material (2.90 + / -0.07), and in addition, the molecular weight of the high-F-value oligopeptide is 1t; peptides with the molecular weight of 1000 Da account for 95-99.40%, the characteristics of oligopeptides are met, the molecular weight is small, and digestion and absorption are easy. The prepared high-F-value oligopeptide can accelerate metabolism of ethyl alcohol in the liver and protect the liver from being damaged by the ethyl alcohol, and has the effects of dispelling the effects of alcohol and protecting the liver.

The invention relates to the technical field of medicines, in particular to a medical dressing for promoting healing of a diabetic wound and a preparation method of the medical dressing. The medical dressing for promoting healing of the diabetic wound surface is prepared from the following raw materials in percentage by mass: 0.008 to 0.012 percent of stem cell exosome, 0.002 to 0.005 percent of oligopeptide-1, 0.004 to 0.01 percent of jasminin, 0.03 to 0.07 percent of modified CaSi2 nanosheets, 2 to 6 percent of hydroxypropyl chitosan, 0.1 to 0.5 percent of xanthan gum, 0.5 to 5 percent of glycerol and the balance of water. The medical dressing not only can promote wound healing, but also is beneficial to scar repair after wound healing.

The invention discloses a composition containing a polypeptide exosome and application of the composition in preparation of a product with skin repair ability, and particularly relates to the technical field of biology, the composition comprises polypeptide-exosome composite nanoparticles, and a polypeptide containing a GRGDS motif and an umbilical cord Wharton's jelly exosome are covalently crosslinked; the lipid double-layer coating layer comprises phosphatidylcholine, cholesterol and PEG-2000, and the molar ratio of the phosphatidylcholine to the cholesterol to the PEG-2000 is 5: 3: 2; the synergist is prepared from 0.1 to 5 percent of epicatechin gallate and 2 to 8 percent of recombinant collagen III oligopeptide. The nano-liposome is prepared through microfluidic self-assembly and a film hydration-ultrasonic intercalation method, and can be loaded on a degradable microneedle patch, so that targeted slow release of a wound part is realized. Through the synergistic effect of targeted delivery, oxidation resistance and collagen regeneration, the skin repair efficiency is remarkably improved, and the healing time is shortened.

The invention discloses a nano compound based on a CRISPR-Cas9 system and application of the nano compound. The nano compound comprises a histidine oligopeptide-cell penetrating peptide conjugate and an RNP compound composed of sgRNA of a target gene and Cas9 protein, and the histidine oligopeptide-cell penetrating peptide conjugate and the RNP compound are self-assembled under the action of zinc ions to form the nano compound. The nano-composite designed by the invention is simple in structure, and overcomes the defects of high cytotoxicity and large particle size (diameter gt; compared with the prior art, the compound has the advantages that the compound can overcome the defects of complex chemical synthesis, potential immunogenicity and the like, can be used for extracellular-cytoplasm-cell nucleus trinity antivirus, is high in virus removal efficiency and good in antivirus effect, and has high market value and development potential.

A peptide composition for improving tear stains of pets belongs to the field of pet health, is used for improving tear stains of pets, and is characterized by comprising 20-30 parts of sea cucumber peptide, 5-10 parts of casein phosphopeptides, 10-20 parts of soybean peptide, 20-30 parts of corn oligopeptide, 10-20 parts of wheat oligopeptide and 10-20 parts of mussel peptide, the amino acid sequence of common mussel peptide is His-Gly-Ala-Asp-Gly-Val-Pro-Pro-Phe, and the amino acid sequence of the common mussel peptide is His-Gly-Ala-Asp-Gly-Val-Pro-Pro-Phe. The amino acid sequence of the perna viridis peptide is Asp-Ser-Ser-Leu-Pro-Pro-Ser-Arg-Pro-Thr, and the perna viridis peptide has the effects of effectively improving the metabolism of ferriporphyrin, inhibiting the activity of saccharomycetes and reducing the formation of tear stains of pets.

The invention relates to a hemoglobin peptide capable of promoting iron absorption and a preparation method thereof, the mass content of polypeptide in the hemoglobin peptide is greater than 90%, and the hemoglobin peptide contains at least one of FERF and FDGL; the preparation method comprises the following steps: S1, adding trisodium citrate into fresh animal blood, centrifuging, adding a NaCl solution for washing, preparing the solution, and performing high-pressure homogenization wall breaking for 2-5 times to obtain a hemoglobin solution; s2, adding 10-200 U / mL of neutral protease, carrying out heat preservation treatment, then adding 5-50 U / mL of flavourzyme, carrying out enzymolysis, and carrying out enzyme deactivation to obtain an enzymatic hydrolysate; s3, filtering with an ultrafiltration membrane, and collecting permeate; s4, adjusting the pH value, adding into an anion exchange chromatographic column, eluting with a NaCl solution, and collecting the eluent; s5, desalting, concentrating and drying to obtain hemoglobin peptide; and S6, carrying out mass spectrometric detection, and screening bioactive peptides to obtain the oligopeptide with the activity of promoting iron absorption. The hemoglobin peptide obtained by the preparation method disclosed by the invention has relatively good iron chelation capability and iron absorption promoting capability, and the bioavailability of iron ions can be remarkably improved.

The invention relates to the technical field of food, in particular to geobacillus stearothermophilus cKM-Geo, application of the geobacillus stearothermophilus cKM-Geo, a shrimp shell oligosaccharide peptide compound and a preparation method of the shrimp shell oligosaccharide peptide compound. The invention provides geobacillus stearothermophilus cKM-Geo, the strain can grow in an oxygen-free environment, ventilation is not needed in the process, and proteolytic enzyme, cellulase, lipase and chitin deacetylase can be highly secreted and expressed; the invention further provides a preparation method for preparing the shrimp shell oligosaccharide peptide by utilizing geobacillus stearothermophilus cKM-Geo fermentation coupling steam explosion treatment, chitin and protein in the shrimp shell are converted into water-soluble oligopeptide and oligosaccharides, the water-soluble oligopeptide and oligosaccharides are separated from calcium carbonate in the shrimp shell, acid and alkali do not need to be used, in the process, waste water is little, pollution is little, and the method is suitable for industrial production. The resource utilization rate is high.

The present invention refers to a biomimetic minerizable 3D-printing ink, a method for the production of such a biomimetic minerizable 3D-printing ink, a method for the production of a biomineralized 3D-printed article, a biomineralized 3D-printed article as well as the use of a crystallization trigger which is an oligopeptide selected from the group comprising an oligopeptide of the HABP family and an oligopeptide of the P11-family for 3D printing.

The invention belongs to the technical field of genetic engineering, and particularly relates to a mutant P17 oligopeptide and application thereof in preparation of a nano antibody. The amino acid sequence of the mutant P17 oligopeptide is KGFRDEKKRFKNTKG. The mutant P17 oligopeptide is a mutant P17 oligopeptide. The mutant P17 oligopeptide provided by the invention has good chaperone-like activity and dissolution promoting effect, has good applicability to different nano antibody strains, is fused with a nano antibody and is expressed in a prokaryotic expression system, so that the solubility and expression efficiency of the nano antibody in the prokaryotic expression system can be effectively improved, and the application prospect is broad. The soluble yield of the nano antibody is greatly improved; moreover, the mutant P17 oligopeptide is beneficial to keeping the biological activity of the antibody, the nano antibody with high activity and high expression quantity is obtained, favorable conditions are created for large-scale production of the nano antibody, and the mutant P17 oligopeptide has a wide application prospect.