531 results about "Oligopeptide" patented technology

A method for visible light-mediated chemical modification of a polypeptide or protein based on cysteine includes the following steps: allowing a cysteine residue on a cysteine-containing oligopeptide, polypeptide, or protein to undergo a reaction with an activating reagent to generate a free radical precursor in situ; and subjecting the free radical precursor to desulfurization under photocatalytic condition, and allowing the generated free radical intermediate to undergo an addition reaction with an olefin or an alkyne, such that sulfhydryl removal and carbon-carbon bond construction are achieved to produce a chemical modification product of the polypeptide and protein. The method cleverly avoids the influence on the chiral center, and thus the chirality of the amino acid residue can be retained. The method is conducive to well avoiding the occurrence of side reactions, and thus the efficiency of the entire conversion is very high.

The invention discloses a compound composition with a multi-target heart protection function as well as a preparation method and application of the compound composition. The composition is composed of a basic functional oil phase, a core antioxidant / anti-inflammatory phase, a myocardial nutrition and regulation phase and a bioavailability promotion phase in a synergistic manner, and is integrated into a composite cardiac oligopeptide and microencapsulated particle key component substance through a specific enzymolysis and microencapsulation embedding process system. The invention aims to solve the technical problems of single target spot, insufficient synergistic effect of all components, low bioavailability of active components and poor stability of the existing heart protection product, and provides a stable and easily-absorbed composite composition which can realize synergistic effect from multiple channels at the same time. Technical effect verification shows that under the condition of equal-dose sample feeding, the composition can remarkably improve the bioavailability of functional components and shows excellent myocardial cell oxidative damage resistance and myocardial ischemia improvement activity in cell and animal models, the effect is remarkably superior to that of independent use or simple physical mixing of all the components, and the composition is suitable for clinical application. And the substantial progress of synergistic interaction is proved. The composition can be used for developing various medicines, health foods and functional foods beneficial to heart health.

The invention discloses a preparation method and permeation promotion application of a high-purity sponge bone needle, and belongs to the technical field of sponge bone needles. Comprising the following steps: cutting a natural sponge into pieces, soaking in deionized water, carrying out ultrasonic oscillation, filtering, and cleaning to obtain a pretreated sponge; carrying out enzymolysis on the pretreated sponge with alkaline protease to obtain a primary zymolyte; and carrying out enzymolysis with ginger protease to obtain a secondary enzymolysis product, and carrying out vacuum freeze drying to obtain the high-purity sponge spicule. The natural sponge is subjected to enzymolysis treatment through alkaline protease and ginger protease, and the high-purity sponge spicule is obtained; wheat oligopeptide is grafted on the surface of a sponge spicule, and giant salamander skin collagen, a pomegranate seed extract, a lonicerae flos extract, a centella asiatica extract, an early celery extract, an iris tectorum extract, an oyster shell extract and calcium chloride mixed treatment materials are loaded, so that the repairing effect on skin inflammation acne is improved.

The invention discloses collagen polypeptide firming and anti-wrinkle freeze-dried powder, and relates to the technical field of cosmetics. The invention relates to a biological preservative which is prepared from water, butanediol, a bacillus / soybean fermentation product extract, soluble collagen, acetyl hexapeptide-8, panthenol, 1, 2, 4-trimethyl-1, 3-pentanediol and a proper amount of water. The cosmetic is prepared from 1, 2-hexanediol, tetrahydromethylpyrimidine carboxylic acid, hydroxypropyl tetrahydropyrantriol, tremella polysaccharide, saccharide isomeride, a bifidus yeast fermentation product lysate, sodium hyaluronate, DNA sodium, a radix gentianae extract, mannitol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, oligopeptide-1, decapeptide-4, oligopeptide-3, hydrolyzed royal jelly protein, folic acid and disodium hydrogen phosphate. The additive is composed of sodium dihydrogen phosphate, citric acid and sodium citrate. The mask can provide rich nutrients for skin, deeply care the skin, moisturize and relieve the skin, tighten the skin, resist wrinkles and make the skin moisturized, glossy, elastic and tight, and is high in use safety and wide in application prospect.

The invention relates to an external composition for hair growth regulation and a preparation method thereof, and belongs to the technical field of cosmetics and medicines. The composition disclosed by the invention comprises nano mesoporous carbon for enhancing permeation, various surface functional modifications on the nano mesoporous carbon, minoxidil, protein fragments, polypeptides, oligopeptides, amino acids, dutasteride, bimatoprost, traditional Chinese medicine extracts, vitamins, caffeine, nucleosides, growth factors and various auxiliary functional components. Wherein the pore diameter of mesopores of the nano mesoporous carbon for enhancing permeation is 2-30 nm, the total pore volume of the mesopores is 1.5-3.5 cm < 3 > / g, and the particle size of the nano mesoporous carbon is 50-200 nm. The prepared nano mesoporous carbon is granular, controllable in size, stable in structure, free of irritation to skin, capable of keeping inert to various active ingredients in the composition, capable of enhancing absorption and permeation of other ingredients on the skin, wide in application range, capable of compounding various types of active ingredients and high in biological safety.

The invention relates to the technical field of biological medicines, in particular to a recombinant rabies virus nano-antibody fused with a solubilizing oligopeptide and a cell penetrating peptide and application of the recombinant rabies virus nano-antibody in preparation of rabies prevention and treatment products. The recombinant rabies virus nano-antibody fused with the solubilizing oligopeptide and the cell penetrating peptide comprises a humanized rabies virus nano-antibody, and the C end of the humanized rabies virus nano-antibody is fused with a solubilizing tag peptide P17 and the cell penetrating peptide. The recombinant rabies virus nano antibody utilizes a prokaryotic expression system, is low in cost, has excellent cell membrane and BBB penetrating ability, can enter a central nervous system from veins for virus neutralization treatment, and has an efficient passive immune effect.

The invention discloses application of oligopeptide in preparation of a medicine for treating and / or preventing diabetic nephropathy. The oligopeptides comprise oligopeptides with the amino acid sequence of SEQ ID NO: 1: LNLYP and derivative sequences or conservative substitution variants of the oligopeptides. The oligopeptide can significantly improve renal function indexes of mice with diabetic nephropathy, such as UACR, serum creatinine, urea nitrogen and the like, enhance podocyte barrier integrity and inhibit accumulation of advanced glycosylation end products AGEs in kidney tissues, and provides a basis for development of novel drugs for treating diabetic nephropathy.

The present invention provides an α-amino acid ester acyltransferase mutant and its application. The α-amino acid ester acyltransferase mutant includes: (a) a protein having the amino acid sequence shown in SEQ ID NO: 1; (b) a protein having an amino acid mutation at at least one of the following sites in the amino acid sequence in (a): P158, K80, T208, A302, A175, N85, N605, K346, S225, I34, or S348, and having the function of an α-amino acid ester acyltransferase; or (c) a protein having more than 80% homology with the amino acid sequence defined in (a) or (b) and having the function of an α-amino acid ester acyltransferase. The α-amino acid ester acyltransferase mutant of the present invention has the advantages of a broad substrate spectrum and high catalytic activity, can be well used in the synthesis of oligopeptides, has low cost and high yield, and realizes true green chemistry.

The invention relates to a preparation method of small-molecular-weight sea cucumber oligopeptide, the prepared sea cucumber oligopeptide and application, and belongs to the technical field of skin care products. The preparation method of the small-molecular-weight sea cucumber oligopeptide comprises the following steps: preparing sea cucumber fine powder; adding the sea cucumber fine powder into ultrapure water, adding papain and EDTA (Ethylene Diamine Tetraacetic Acid), and carrying out enzymolysis under the assistance of microwaves; adding trypsin and calcium ions, and carrying out enzymolysis under the assistance of microwaves; adding neutral protease and zinc ions, and performing enzymolysis under the assistance of microwaves to obtain final sea cucumber enzymatic hydrolysate; and decolorizing, separating and purifying, and freeze-drying to obtain the powdery small-molecular-weight sea cucumber oligopeptide. According to the preparation method of the small-molecular-weight sea cucumber oligopeptide, microwave heating and enzymolysis reaction are combined, the hydrolysis degree of a peptide chain is regulated and controlled by controlling an enzyme hydrolysis sequence and enzymolysis conditions, the small-molecular-weight sea cucumber oligopeptide with relatively concentrated and moderate molecular weight is prepared, and good biological activity of the sea cucumber oligopeptide is kept.

The invention belongs to the technical field of biological medicine, and relates to a composite polypeptide composition as well as a preparation method and application thereof. According to the technical scheme, pH responsive polymer loaded oligopeptide-1, acetyl heptapeptide-4 and palmitoyl tripeptide-1 are adopted as a core, hyaluronic acid is adopted for wrapping, a hyaluronic acid layer adsorbs palmitoyl tripeptide-8, heptapeptide-6 and decapeptide-4, and the composite polypeptide with a core-shell structure is prepared and used for treating inflammatory skin diseases. In the acute stage of inflammation, the active ingredients on the outer layer quickly inhibit the activation of proinflammatory factors and immune cells and relieve red, swollen and heat pain, and the gel outer layer formed by hyaluronic acid realizes continuous release, prolongs the action time and supplements moisture to the skin; the pH-responsive polymer of the inner layer releases active ingredients at the inflammation part, so that relapse is prevented, and the skin barrier function is repaired. The composition plays a role from multiple dimensions of inflammation regulation, barrier repair, oxidation resistance and the like through a multi-layer structure design and a multi-component synergistic effect.

The invention provides lucid ganoderma oligopeptide with an anti-tumor effect and a preparation method thereof. The three oligopeptides are separated and purified from the ganoderma lucidum for the first time, and the oligopeptides have broad-spectrum anti-tumor activity, can effectively inhibit tumor cell proliferation, have good biological compatibility and have great application prospects.

The invention discloses an essence composition containing lipidosome with an acne removing effect as well as a preparation method and application of the essence composition. The essence composition comprises the following components: 2-30% of lipidosome with the acne removing effect, 0.5-15% of a skin conditioner, 5-40% of a humectant, 0.1-3% of a preservative, 0.15-10% of a thickening agent, 0.05-5% of a chelating agent, 0.01-3% of a pH regulator and deionized water. The lipidosome with the acne removing effect is prepared from the following components in percentage by weight: 1.5 to 2.5 percent of white willow bark extract, 0.3 to 0.7 percent of Pinosine, 2 to 4 percent of rhizoma bletillae root extract, 4 to 6 percent of nicotinamide, 0.15 to 0.35 percent of oligopeptide-3, 7 to 12 percent of lecithin and 1 to 2 percent of cholesterol. In the preparation process, the lipidosome with the acne removal effect is reasonably compounded with other auxiliary material matrixes, so that efficient transmission and absorption of active ingredients can be promoted, irritation to skin can be effectively relieved, meanwhile, the overall effect of the essence is enhanced, the essence has mildness and high efficiency, and the high-efficiency requirement of consumers for the acne removal skin care product is met.

The invention discloses a composition containing a polypeptide exosome and application of the composition in preparation of a product with skin repair ability, and particularly relates to the technical field of biology, the composition comprises polypeptide-exosome composite nanoparticles, and a polypeptide containing a GRGDS motif and an umbilical cord Wharton's jelly exosome are covalently crosslinked; the lipid double-layer coating layer comprises phosphatidylcholine, cholesterol and PEG-2000, and the molar ratio of the phosphatidylcholine to the cholesterol to the PEG-2000 is 5: 3: 2; the synergist is prepared from 0.1 to 5 percent of epicatechin gallate and 2 to 8 percent of recombinant collagen III oligopeptide. The nano-liposome is prepared through microfluidic self-assembly and a film hydration-ultrasonic intercalation method, and can be loaded on a degradable microneedle patch, so that targeted slow release of a wound part is realized. Through the synergistic effect of targeted delivery, oxidation resistance and collagen regeneration, the skin repair efficiency is remarkably improved, and the healing time is shortened.

The invention discloses a nano compound based on a CRISPR-Cas9 system and application of the nano compound. The nano compound comprises a histidine oligopeptide-cell penetrating peptide conjugate and an RNP compound composed of sgRNA of a target gene and Cas9 protein, and the histidine oligopeptide-cell penetrating peptide conjugate and the RNP compound are self-assembled under the action of zinc ions to form the nano compound. The nano-composite designed by the invention is simple in structure, and overcomes the defects of high cytotoxicity and large particle size (diameter gt; compared with the prior art, the compound has the advantages that the compound can overcome the defects of complex chemical synthesis, potential immunogenicity and the like, can be used for extracellular-cytoplasm-cell nucleus trinity antivirus, is high in virus removal efficiency and good in antivirus effect, and has high market value and development potential.

A peptide composition for improving tear stains of pets belongs to the field of pet health, is used for improving tear stains of pets, and is characterized by comprising 20-30 parts of sea cucumber peptide, 5-10 parts of casein phosphopeptides, 10-20 parts of soybean peptide, 20-30 parts of corn oligopeptide, 10-20 parts of wheat oligopeptide and 10-20 parts of mussel peptide, the amino acid sequence of common mussel peptide is His-Gly-Ala-Asp-Gly-Val-Pro-Pro-Phe, and the amino acid sequence of the common mussel peptide is His-Gly-Ala-Asp-Gly-Val-Pro-Pro-Phe. The amino acid sequence of the perna viridis peptide is Asp-Ser-Ser-Leu-Pro-Pro-Ser-Arg-Pro-Thr, and the perna viridis peptide has the effects of effectively improving the metabolism of ferriporphyrin, inhibiting the activity of saccharomycetes and reducing the formation of tear stains of pets.

The invention relates to a hemoglobin peptide capable of promoting iron absorption and a preparation method thereof, the mass content of polypeptide in the hemoglobin peptide is greater than 90%, and the hemoglobin peptide contains at least one of FERF and FDGL; the preparation method comprises the following steps: S1, adding trisodium citrate into fresh animal blood, centrifuging, adding a NaCl solution for washing, preparing the solution, and performing high-pressure homogenization wall breaking for 2-5 times to obtain a hemoglobin solution; s2, adding 10-200 U / mL of neutral protease, carrying out heat preservation treatment, then adding 5-50 U / mL of flavourzyme, carrying out enzymolysis, and carrying out enzyme deactivation to obtain an enzymatic hydrolysate; s3, filtering with an ultrafiltration membrane, and collecting permeate; s4, adjusting the pH value, adding into an anion exchange chromatographic column, eluting with a NaCl solution, and collecting the eluent; s5, desalting, concentrating and drying to obtain hemoglobin peptide; and S6, carrying out mass spectrometric detection, and screening bioactive peptides to obtain the oligopeptide with the activity of promoting iron absorption. The hemoglobin peptide obtained by the preparation method disclosed by the invention has relatively good iron chelation capability and iron absorption promoting capability, and the bioavailability of iron ions can be remarkably improved.

The invention relates to the technical field of food, in particular to geobacillus stearothermophilus cKM-Geo, application of the geobacillus stearothermophilus cKM-Geo, a shrimp shell oligosaccharide peptide compound and a preparation method of the shrimp shell oligosaccharide peptide compound. The invention provides geobacillus stearothermophilus cKM-Geo, the strain can grow in an oxygen-free environment, ventilation is not needed in the process, and proteolytic enzyme, cellulase, lipase and chitin deacetylase can be highly secreted and expressed; the invention further provides a preparation method for preparing the shrimp shell oligosaccharide peptide by utilizing geobacillus stearothermophilus cKM-Geo fermentation coupling steam explosion treatment, chitin and protein in the shrimp shell are converted into water-soluble oligopeptide and oligosaccharides, the water-soluble oligopeptide and oligosaccharides are separated from calcium carbonate in the shrimp shell, acid and alkali do not need to be used, in the process, waste water is little, pollution is little, and the method is suitable for industrial production. The resource utilization rate is high.

The invention provides a preparation method and application of a composite polypeptide metagen solid preparation, the composite polypeptide metagen solid preparation comprises a metagen and a peptide component, the metagen is prepared by fermenting lactobacillus plantarum nbk-MA2 and lactobacillus kefiranosus subsp.kefiranosus ZW3, the peptide component comprises albumin polypeptide, cordyceps sobolifera peptide, ginseng peptide, corn oligopeptide and collagen peptide, and the composite polypeptide metagen solid preparation is prepared by fermenting lactobacillus plantarum nbk-MA2, lactobacillus kefiranosus subsp.kefiranosus ZW3 and lactobacillus kefiranosus subsp.kefiranosus ZW3. The protein powder is prepared from protein powder raw materials through protease treatment. According to the composite polypeptide metagen solid preparation, the synergistic interaction effect can be achieved through compounding of the composite metagen and the peptide assembly, immunity is improved, tumor growth and migration are inhibited, tumor cell apoptosis is promoted, toxic and side effects are avoided, and the composite polypeptide metagen solid preparation is a food-borne functional product with good application prospects.

The invention relates to a polysaccharide and oligopeptide composition and application thereof in promoting plant growth. The polysaccharide and oligopeptide composition comprises the following components: xanthan gum, algal polysaccharides, a grifola frondosa extract, soybean oligopeptide, wheat oligopeptide, corn oligopeptide, modified silicon dioxide, itaconic acid, indolebutyric acid, glycine, glutamic acid, citric acid and fatty alcohol-polyoxyethylene ether. Wherein the modified silicon dioxide is obtained by reacting silicon dioxide with an acrylate-based silane coupling agent and then reacting with acrylic acid esterified humic acid. According to the plant growth promoter, the modified silicon dioxide is compounded with various substances such as polysaccharide and oligopeptide, the components are good in compatibility, the stability of the product is improved, the action time is prolonged, the absorption efficiency is improved, plant growth can be efficiently promoted, the defects of existing products are overcome, and the plant growth promoter has important significance on development of plant growth promoter products.

The invention provides a combined lactobacillus salivarius YS-UR90 and a preparation method of the combined lactobacillus salivarius YS-UR90. The saliva combined lactobacillus provided by the invention can secrete protease and glycosidase, and the HCT and FLS gene expression levels of the saliva combined lactobacillus are high, so that the saliva combined lactobacillus can promote phenolic acid active ingredients to generate biotransformation during fermentation, and the proportion of chlorogenic acid and the like in fermentation liquor is increased. For example, marine fish oligopeptide and casein phosphopeptides are used as a composite nitrogen source, dandelion powder is used as a functional substrate, and a composite fermentation product obtained through fermentation of saliva and lactobacillus has the effect of promoting uric acid excretion and has a certain protection effect on kidney injury. The compound fermentation product has the effect of improving hyperuricemia mainly by inhibiting XOD activity, increasing serum urea nitrogen level, improving defect expression of uric acid synthesis gene HPRT1, inhibiting expression of uric acid reabsorption URAT1 and GLUT9 genes and improving expression of excretion genes OAT1 and ABCG2.

The invention belongs to the technical field of agricultural fertilizers, and particularly relates to a cold-resistant feather amino acid leaf fertilizer and a preparation method thereof.The cold-resistant feather amino acid leaf fertilizer is prepared from, by mass, 86%-95% of feather source amino acid-oligopeptide mixed liquor, 3%-8% of L-lysine, 1%-3% of chelated microelements, 0.5%-1.0% of trehalose, 0.5%-1.2% of polyether modified trisiloxane, 0.5%-1.0% of sodium polyaspartate and 0.1%-0.5% of preservative, wherein the pH (Potential of Hydrogen) value is 5.0 to 6.5; the chelated trace elements are EDTA (Ethylene Diamine Tetraacetic Acid) or amino acid chelates of at least three elements of zinc, iron, manganese, copper and boron. By optimizing a feather pretreatment process and a multi-enzyme synergistic system design and combining functional component compounding, the hydrolysis efficiency and the product activity are remarkably improved, and meanwhile, the permeability and the nutrient transfer effect of the foliar fertilizer under a low-temperature condition are enhanced.

The invention discloses a freeze-dried essence with a repairing effect and a preparation method, and belongs to the field of skin care products, and the freeze-dried essence is composed of freeze-dried powder and an independent solvent liquid. The freeze-dried powder contains 0.5-3 parts of palmitoyl oligopeptide, 3-8 parts of I-type collagen, 1-5 parts of III-type collagen, 0.1-1 part of chitosan, 1-3 parts of secondary variable-temperature fermentation liquor of betula platyphylla and 2-8 parts of a freeze-drying protective agent; the solvent liquid is prepared from 0.5 to 3 parts of hyaluronic acid and 0.05 to 0.5 part of sodium polyglutamate. The preparation method comprises the following steps: a, adding fermentation liquor after dissolving; b, adding nano liposome and a protective agent; c, quickly freezing to-55 + / -2 DEG C by liquid nitrogen, and preserving heat for 2.5-3.5 hours; d, drying for 9-11 hours under the vacuum of 15-25 Pa and at the temperature of-18 + / -2 DEG C; and e, the temperature is increased to 15 + / -2 DEG C in a stepped mode, vacuum is smaller than or equal to 20 Pa, and drying is conducted for 18- Through scientific formula design, multiple effects of efficient repairing, anti-aging and relieving are achieved.

The present invention relates to a compound binding to an endogenous receptor, said compound comprising (i) an oligopeptide comprising a dipeptide with Trp being the C-terminal amino acid of said dipeptide, wherein said Trp is replaced with an α-amino acid Xaa2, whereby the stability in serum or plasma of the peptide bond connecting Xaa2 to the N-terminally adjacent amino acid is increased as compared to the peptide bond connecting Trp to the N-terminally adjacent amino acid in an otherwise identical compound; and (ii) a moiety capable of generating therapeutically effective radiation, said moiety being covalently bound to said oligopeptide.

The invention relates to the technical field of exosomes, and particularly discloses a cosmetic anti-aging mesenchymal stem cell exosome composition, which comprises a therapeutically effective amount of mesenchymal stem cell-derived exosome, the functional carrier comprises a transdermal absorption enhancer and an antioxidant stabilizer; at least one synergistic active component selected from the group consisting of small molecule peptides, plant-derived antioxidants, hyaluronic acid or derivatives thereof; the small molecule peptide is one of oligopeptide-1 and palmitoyl tripeptide-5; the plant source antioxidant is one of resveratrol and ferulic acid; wherein the exosome and the synergistic active component are compounded through a phospholipid bilayer fusion technology; through a phospholipid bilayer fusion technology, the mesenchymal stem cell exosome and a synergistic active component are directionally compounded, an exosome membrane structure is used as a natural carrier, the active component is effectively prevented from being degraded, and meanwhile, the bioavailability is improved by utilizing the targeting property of the exosome membrane structure.

The invention relates to the technical field of skin care products. The invention aims to provide a split storage type novel skin-brightening skin-care product combined by oligopeptide freeze-dried powder and essence. The split storage type novel skin-brightening skin-care product comprises multiple oligopeptide freeze-dried powder and solvent essence, the multi-oligopeptide freeze-dried powder is prepared from the following raw materials in parts by weight: 90 to 95 parts of water, 1 to 2 parts of glutathione, 1 to 2 parts of mannitol, 1 to 2 parts of carnosine, 0.5 to 1 part of ergothioneine, 0.5 to 1 part of vitamin C and 0.0005 to 0.001 part of a multi-peptide-zinc chelate. The multi-peptide-zinc chelate is prepared from the following raw materials in parts by weight: 1-2 parts of hexapeptide-2, 1-2 parts of nonapeptide-1, 1-2 parts of dipeptide-2, 1-2 parts of tripeptide-10 and 2-3 parts of zinc oxide. According to the invention, various peptides with small molecular weights are chelated with zinc, the characteristics of good permeability and strong skin affinity of the small molecular peptides are fully played, the effects of zinc and other components are effectively played, and the overall use effect is greatly improved. Through compounding of multiple components, the moisturizing and brightening mask has multiple effects of moisturizing, good permeability, whitening, brightening and the like.

The invention discloses a modular tumor microenvironment response polypeptide and a preparation method and application thereof. The modular tumor microenvironment response polypeptide is composed of the following functional modules: a targeting and chemotactic module used for targeting tumor cell surface p32 protein and tumor lymphatic vessels and promoting tumor tissue penetration and enrichment; the microenvironment double-response module is used for being cut by MMP-2 / 9 enzyme and broken by high-concentration glutathione GSH in a tumor microenvironment to trigger self-assembly; the self-assembly driving module is used for exposing after the microenvironment responds to activation and driving short peptides to be self-assembled to form a nano structure; a killing module: a pro-apoptotic peptide PAD used for targeting a mitochondrial membrane and inducing tumor cell apoptosis; and the stability optimization module is used for enhancing the degradation resistance of the oligopeptide. By integrating targeted delivery, enzyme digestion response, acid-sensitive release and stabilizing structures, the problems of insufficient targeting property, inaccurate release, poor stability, diagnosis-treatment splitting and the like of the anti-tumor drug and the developing agent are solved.

The invention discloses a quaternary synergistic water-soluble fertilizer synergist as well as a preparation method and application thereof, and realizes deep coupling of chemical growth promotion, biological activation and physical slow release, multi-component synergistic interaction and efficient nutrient utilization based on a four-dimensional synergistic mechanism of chitosan oligosaccharide-oligopeptide-bioactive alkali-functional microbial agent. Wherein the modified chitosan oligosaccharide enhances the chelating ability through molecular structure optimization, and forms a nano-scale composite system with the oligopeptide, so that the absorption and transfer efficiency of nutrients on leaf surfaces and root systems is remarkably improved. The functional microbial agent and chitosan oligosaccharide cooperate to activate soil enzyme activity and promote release of insoluble phosphorus and potassium, so that the utilization rate of nitrogen, phosphorus and potassium is remarkably increased compared with that of a traditional fertilizer, and nutrient loss is effectively reduced. The quaternary synergistic water-soluble fertilizer synergist provided by the invention is high in compatibility, can be directly compounded with a commercially available water-soluble fertilizer for use, is suitable for diversified planting scenes of facility agriculture, field crops and commercial crops, and has large-scale popularization potential.

The invention belongs to the field of functional polypeptides, and discloses an elastinase inhibitory peptide and a preparation method thereof. The elastase inhibitory peptide comprises nine types of oligopeptides from crayfish, and the amino acid sequences of the oligopeptides are respectively GASGPAGPRGPVGVA, IDGF, GAAGPAGPRGPAGPA, SGPAGPA, GPAGPRGPVGVA, SGPAGPRGPVGVA, VGPQGASGPLGPA and IGPA. The invention further discloses a preparation method of the elastase inhibitory peptide. The elastinase inhibitory peptide disclosed by the invention can be chemically synthesized and can also be directionally prepared from fish gelatin enzymolysis products. The elastase inhibitory peptide disclosed by the invention has good binding force with elastase and has remarkable elastase inhibitory activity.

Described herein are compositions comprising an extract from Hibiscus genus and an extract from damaged plant parts from the Moringaceae genus, and methods for stimulating collagen synthesis in skin cells. Also described herein are compositions comprising an extract from Hibiscus genus, an extract from damaged plant parts from the Moringaceae genus, and a complex of actives comprising at least one oligopeptide, at least one extract from the Laminaria genus, and whey protein.

The invention provides a retinyl oligopeptide derivative with anti-aging and wrinkle fading functions and application thereof, and belongs to the technical field of polypeptide derivative application, the retinyl oligopeptide derivative is obtained by connecting a carboxyl terminal of retinoic acid and an amino terminal of oligopeptide, and has a structure as shown in a formula I. In the formula I, n is 1 or 2; l is oligopeptide. The retinoic acid modified oligopeptide derivative provided by the invention has good solubility in both a water phase and an oil phase, is beneficial to product formula design in an application process, has good antioxidant activity and skin cell injury protection activity, can reduce MMP2 and TNF alpha expression increased by ROS in skin fibroblasts, and has a good application prospect. The composition has excellent activity of promoting collagen expression, and has good effects of resisting oxidation, repairing skin injury and smoothing wrinkles when being used for cosmetics or skin care products.