17 results about "ASIATIC ACID" patented technology

The present application relates to the technical field of asiatic acid, and particularly relates to an asiatic acid derivative and a preparation method.The asiatic acid derivative provided by the present application has R which is a straight chain or a branched chain with 1-24 carbon atoms, or a saturated or unsaturated monohydric alcohol alkyl formed by removing a hydroxyl group.The R group of the asiatic acid derivative of the present application has higher water solubility and oil solubility, especially oil solubility, so that the asiatic acid derivative has obviously enhanced liposolubility compared with asiatic acid, is easily dissolved in natural oil, alkane and other ester solvents, and has obviously enhanced biological activity, so that the asiatic acid derivative of the present application has high availability, improves the inhibition effect of the asiatic acid derivative on EDNRA and TRPV1, and further improves the anti-tumor effect, and the asiatic acid derivative has no safety risk.

The present application relates to the technical field of nucleic acid drug preparation, and discloses a lipid nanoparticle based on asiatic acid and a preparation method and application thereof, the lipid nanoparticle is composed of ionizable cationic lipids, structural lipids, neutral lipids, PEG lipids and asiatic acid with a molar ratio of 10-85:20-60:1-30:0.1-10:0.1-50, and is used as a delivery carrier of nucleic acid drugs for delivering nucleic acid drugs. The present application innovatively uses natural product asiatic acid to partially replace the structural lipids (such as cholesterol) in the traditional LNP formula, significantly optimizes the micro morphology, and greatly enhances the transfection and expression efficiency of mRNA in vitro and in vivo. The mRNA vaccine based on the carrier can induce the body to produce high-titer specific antibodies and significantly enhance the cytotoxic T lymphocyte (CTL) killing activity. The LNP system performs outstandingly in the field of tumor immunoprevention and treatment, and shows superior conversion application potential and broad market prospects.

The present application provides a kind of epidermis repair gel dressings and its preparation method, it is related to dressing field.The epidermis repair gel dressings includes repair liquid, 100 parts of the repair liquid includes composite sodium hyaluronate 0.1-0.3 parts, carbomer 0.02-0.06 parts, glycerol 1-4 parts, pentanediol 1-3 parts, composite acid 0.05-0.1 parts, composite peptide 0.01-0.03 parts and the balance purified water by weight parts;The composite sodium hyaluronate is composed of sodium hyaluronate, hydrolyzed sodium hyaluronate and sodium hyaluronate crosslinked polymer;The composite acid is prepared from ursolic acid, hydroxyl asiatic acid, lipoic acid;The composite peptide is prepared from thirteen peptides and palmitoyl pentapeptide-3.The epidermis repair gel dressing of the present application has better repair effect on acne lesions, not only inhibits active propionibacterium acnes, but also can reduce the expression of inflammatory factors, can deeply repair damaged basal structure, maximumly reduce the degree of acne lesions, and restore to close normal skin.

The invention relates to the technical field of nucleic acid medicine preparations, and discloses lipid nanoparticles based on asiatic acid and a preparation method and application thereof.The lipid nanoparticles are composed of ionizable cationic lipid, structural lipid, neutral lipid, PEG lipid and asiatic acid according to the molar ratio of (10-85): (20-60): (1-30): (0.1-10): (0.1-50) and serve as delivery carriers of nucleic acid medicine. For delivery of nucleic acid drugs. According to the invention, a natural product asiatic acid is creatively adopted to partially replace a structural lipid (such as cholesterol) in a traditional LNP formula, and the micromorphology of the LNP is obviously optimized, so that the in-vivo and in-vitro transfection and expression efficiency of mRNA is greatly enhanced. The mRNA vaccine constructed based on the vector can induce an organism to generate a high-titer specific antibody, and the killing activity of cytotoxic T lymphocytes (CTL) is remarkably enhanced. The LNP system has outstanding performance in the field of tumor immunoprophylaxis and treatment, and shows excellent transformation application potential and wide market prospect.

The invention discloses an asiatic acid saponin compound and medical application thereof. The asiatic acid saponin compound comprises a compound with a structural formula as shown in a formula (I), and pharmaceutically acceptable salt or ester or solvate of the compound. The compound shown in the formula (I) has anti-inflammatory and anti-ulcer activity, so that the compound can be used for preparing medicines for preventing or treating inflammatory diseases or ulcerative diseases.

The application provides a skin bacteriostatic repair emulsion and a preparation method thereof, and relates to the field of cosmetics. The skin bacteriostatic repair emulsion comprises an oil phase, an aqueous phase and an emulsifier, the components of the oil phase are dispersed in the aqueous phase in the form of droplets under the action of the emulsifier, and the particle size of the droplets is not more than 200 nm; the components of the oil phase comprise at least one of cinnamaldehyde octadecylamine complex, paeonol octadecylamine covalent coupling compound and asiatic acid. The embodiment of the application can well improve the physicochemical properties of cinnamaldehyde, paeonol and asiatic acid, so that the cinnamaldehyde, the paeonol and the asiatic acid can exist stably and in a large amount in actual use, and have a good treatment effect on acne.

The invention discloses an asiatic acid saponin compound and medical application thereof. The asiatic acid saponin compound comprises a compound with a structural formula as shown in a formula (I), and pharmaceutically acceptable salt or ester or solvate of the compound. The compound shown in the formula (I) has anti-inflammatory and anti-ulcer activity, so that the compound can be used for preparing medicines for preventing or treating inflammatory diseases or ulcerative diseases.

The present application relates to the technical field of asiatic acid, and particularly relates to an asiatic acid derivative and a preparation method. The asiatic acid derivative provided by the present application has R which is an alkyl group formed by removing a hydroxyl group from a saturated or unsaturated monohydric alcohol, and the saturated or unsaturated monohydric alcohol includes one of ethanol, octanol, hexadecanol, stearyl alcohol, 2-hexyl-1-decanol, decyltetradecin, oleyl alcohol, cis,cis-9,12-octadecadienol and bisabolol. The asiatic acid derivative of the present application has higher water solubility and oil solubility, especially oil solubility, so that the asiatic acid derivative has obviously enhanced liposolubility, is easily soluble in natural oil, alkane and other ester solvents, and has a significant inhibitory effect on EDNRA and TRPV1 and obviously enhanced bioactivity. The asiatic acid derivative of the present application has high availability, and the asiatic acid derivative has no safety risk.

The invention discloses a recombinant saccharomyces cerevisiae strain with high yield of asiatic acid and a construction method of the recombinant saccharomyces cerevisiae strain. The method comprises the following steps: integrating a C-2alpha hydroxylated cytochrome P450 oxidase gene AmCYP716C53 from avicennia marina and a C-23 hydroxylated cytochrome P450 oxidase gene CaCYP716E19 from centella asiatica to a chromosome 911b site of a chassis strain ZY35 by utilizing a CRISPR-Cas9 gene editing technology, so as to obtain ZSA01; then, genes MCH5, FLX1, RIB1, FMN1 and FAD1 are integrated to the 1014a site of the ZSA01, and ZSA02 is obtained; finally, the AmCYP716C53 is integrated to the HMX1 site of the ZSA02, and an engineering strain ZSA03 is constructed. Fermentation results show that ZSA03 reaches 1g / L in a 5L fermentation tank, and the ZSA03 has high-efficiency synthesis and industrial application potential.

The invention relates to the technical field of biological medicine manufacturing, in particular to a traditional Chinese medicine compound gel containing bioactive organic components and capable of synergistically repairing hypertrophic scars based on four-season qi and a preparation method of the traditional Chinese medicine compound gel. The compound gel is formed by mixing a bottle A base matrix and a bottle B four-season additive according to the ratio of 9: 1, the base substrate of the bottle A contains various bioactive organic components such as a salvia miltiorrhiza extract, asiatic acid, bioactive hirudin, perilla oil and the like; according to the four-season additive in the bottle B, corresponding traditional Chinese medicine extracts are selected according to seasons, the preparation method adopts a biotechnology process including supercritical COextraction, a biocompatibility nanometer co-grinding technology and other modern biotechnologies, the transdermal absorption rate of mineral components is remarkably increased, the anti-fibrosis effect is enhanced through a hirudin-perilla oil soluble synergistic system, and the anti-fibrosis effect is improved. The four-season differentiated formula realizes accurate repair of preventing wind evil in spring, clearing blood heat in summer, moistening dryness injury in autumn and dredging collaterals in winter.

The application discloses a Centella asiatica transcription factor CaDREB12 coding gene, protein, recombinant carrier and application thereof, and belongs to the technical field of molecular biology and plant genetic engineering. In the prior art, there is a lack of transcription factor gene resources capable of positively regulating biosynthesis of a main active component, i.e., hydroxyl asiatic acid, of Centella asiatica. The nucleotide sequence of the CaDREB12 coding gene provided in the application is shown as SEQ ID NO. 1. By using the gene to construct a recombinant expression carrier and transform Centella asiatica, transgenic hairy roots with greatly increased content of hydroxyl asiatic acid can be obtained, and the application provides important gene resources and technical foundations for metabolic engineering directional improvement of medicinal active components of Centella asiatica, innovation of germplasm resources and research of synthetic biology.

The invention discloses an asiatic acid nano-structure lipid carrier and a soluble microneedle drug delivery system, which are characterized in that the dosage form of asiatic acid is designed and optimized, and a drug is prepared into the nano-structure lipid carrier to improve the absorption of the drug, so that the drug effect of asiatic acid is maximized, and the drug delivery efficiency is improved. A soluble microneedle drug delivery system based on a soluble microneedle is designed, the microneedle is combined with an asiatic acid nano-structure lipid carrier, thickened scar tissue is punctured in a novel local percutaneous delivery mode, percutaneous absorption of insoluble traditional Chinese medicine ingredients can be promoted, and the curative effect is improved. According to the microneedle-nanostructure lipid carrier, the physical and chemical infiltration enhancing advantage of the microneedle-nanostructure lipid carrier is fully played, the percutaneous infiltration amount is increased, the problem of low drug absorption efficiency caused by scars is solved, and the adverse reaction of the drug is reduced by utilizing the slow release characteristic of the nanostructure lipid carrier, so that the effects of enhancing efficacy and reducing toxicity are achieved.

The invention discloses a composition containing ganoderma sinensis fermented filtrate and application of the composition in skin repairing and whitening. The invention belongs to the technical field of cosmetics, and provides a composition which comprises ganoderma sinensis fermentation filtrate, tocopheryl acetate, ascorbyl glucoside, bisabolol, madecassic acid and ferulic acid. The natural multi-effect Ganoderma sinensis fermentation filtrate is taken as a core basis, two core pain points of skin barrier damage and melanin deposition are solved in a targeted manner, specific components are compounded, and multi-dimensional synergistic interaction of skin repair'anti-inflammation-infiltration promotion-active reconstruction 'and whitening'enzyme inhibition-reduction-stabilization-antioxidation' is realized, so that the skin care effect is improved, and the skin care effect is achieved. Meanwhile, the skin microenvironment and the stability of active ingredients are optimized, a synergistic effect is achieved through multiple channels, multi-directional repairing is achieved, the obvious effects of promoting skin barrier repairing, reducing inflammatory hyperpigmentation and inhibiting melanogenesis are achieved, and the composition can be used for preparing more cosmetics for repairing skin and whitening and brightening the skin.

This invention discloses a microcapsule with time-released baicalein and asiatic acid nanoparticles, its preparation method, and its application. The preparation method of the microcapsule includes: firstly, synthesizing baicalein nanoparticles and asiatic acid liposomes separately; then, using microfluidic electrospray technology, preparing core-shell structured microdroplets with a sodium alginate solution loaded with baicalein nanoparticles as the outer phase and a sodium alginate solution loaded with asiatic acid liposomes as the inner phase; finally, solidifying with calcium chloride solution to obtain core-shell microcapsules. The microcapsule of this invention utilizes its unique core-shell structure to preferentially release baicalein nanoparticles in the shell layer in a physiological environment to achieve early antibacterial effects, followed by the release of asiatic acid liposomes in the core layer to promote later cell migration and tissue repair, achieving time-sequential drug release that matches the wound healing process. This microcapsule significantly improves the bioavailability and delivery controllability of poorly soluble traditional Chinese medicine active ingredients, and has broad application prospects in the field of wound repair.

The invention provides non-animal-derived carboxyethyl chitosan hydrogel for injection as well as a preparation method and application of the non-animal-derived carboxyethyl chitosan hydrogel, and belongs to the technical field of hydrogel. The preparation method comprises the following steps: modifying bacterial source chitosan with acrylic acid, and coupling the modified bacterial source chitosan with silk fibroin peptide to prepare silk fibroin peptide / non-animal source carboxyethyl chitosan; oligomeric hyaluronic acid is oxidized, cholesterol is modified and used for coating bisabolol, squalene and asiatic acid, and oxidized hyaluronic acid modified lipidosome is prepared; the non-animal-derived carboxyethyl chitosan hydrogel for injection is prepared by mixing the non-animal-derived carboxyethyl chitosan with carbohydrazide modified gelatin, silk fibroin peptide / non-animal-derived carboxyethyl chitosan and oxidized hyaluronic acid, has good moisturizing, antibacterial and hemostatic properties, provides a moist environment for wound surfaces, prevents microbial infection, promotes granulation tissue formation, and has a good application prospect. The drug release rate is changed by matching with the change of the pH value of the skin surface, the wound healing process is shortened, and the high water content of the hydrogel is combined with the moisturizing property of the chitosan to prevent secondary injury caused by wound adhesion.

The invention relates to a centella asiatica extract with an efficient soothing effect and a preparation method of the centella asiatica extract, and belongs to the technical field of cosmetics. A eutectic solvent system is adopted, tetraethylammonium chloride and sodium sulfamate serve as hydrogen bond acceptors, gluconic acid and glycerol serve as hydrogen bond donors, a stable eutectic solvent is formed through hydrogen bond interaction, and the dissolving capacity of different polar components is effectively improved. The deep-eutectic solvent system is especially suitable for efficient extraction of madecassoside and asiaticoside, asiatic acid and madecassoside in a certain proportion can be extracted under optimized conditions, the steps of resin adsorption, elution, concentration, crystallization and the like are combined, and a high-purity centella asiatica compound can be obtained. Therefore, the extract has anti-inflammatory and soothing effects.

The invention discloses a preparation method of asiatic acid and curcumin co-loaded liposome, which comprises the following steps: respectively weighing egg yolk lecithin, cholesterol, DSPE-mPEG2k, asiatic acid and curcumin; the preparation method comprises the following steps: dissolving raw material components in a mixed solvent to obtain a first solution; removing the mixed solvent in the first solution by adopting a rotary evaporator, and forming a solid lipid membrane on the inner wall of the round-bottom flask; a phosphate buffer solution is added to the lipid membrane and stirred, so that the lipid membrane is hydrated to form LipoAA / Cur; continuously extruding the LipoAA / Cur through a series of polycarbonate films with different pore diameters by using a liposome extruder; purifying and removing the free medicine by using an ultrafiltration tube to obtain the purified asiatic acid and curcumin co-loaded lipidosome; according to the invention, the common encapsulation efficiency, the drug loading capacity, the solubility and the stability of the asiatic acid and the curcumin can be improved.