Method for extracting, separating and purifying chemical components of walnut green seedcases and application of chemical components

A technology of walnut green husk and purification method, applied in the separation/purification of carboxylic acid compounds, separation/purification of carbonyl compounds, chemical instruments and methods, etc., can solve the problems of no research reports, etc., and achieve the effect of strong growth inhibitory activity

Inactive Publication Date: 2019-05-10
新乡医学院三全学院
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  • Claims
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  • Method for extracting, separating and purifying chemical components of walnut green seedcases and application of chemical components
  • Method for extracting, separating and purifying chemical components of walnut green seedcases and application of chemical components
  • Method for extracting, separating and purifying chemical components of walnut green seedcases and application of chemical components

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Embodiment

[0023]In order to further understand the chemical constituents of walnut green peels from different origins and explore their medicinal value, this experiment studied the chemical constituents of walnut green peels collected from Checun Town, Songxian County, Luoyang City, Henan Province, and isolated 13 compounds, which were identified as : 3,5-dimethoxy-4-hydroxy-benzoic acid-7-O-β-D-glucoside (1), myricananin F (2), β-sitosterol (3), oleanolic acid ( 4), walnut ketone (5), Asiatic acid (6), hedera saponin (7), β-carotin (8), (4R)-4,8-dihydroxy-α-tetralone- 4-O-β-D-glucopyranoside (9), 3β,23-dihydroxy-12-ene-28-ursolic acid (10), ursolic acid (11), syringic acid (12), Juglanoside B(13). Among them, compounds 1, 2, 6, 7, 9, and 10 were isolated from this plant for the first time, and the six compounds were also isolated from this genus for the first time.

[0024] Using MTT staining method, compounds 1~6, 9, 11 and 13 were respectively mixed with human liver cancer cells (H...

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Abstract

The invention discloses a method for extracting, separating and purifying chemical components of walnut green seedcases and application of the chemical components. According to the method, 13 compounds are separated from the walnut green seedcases and respectively identified as 3,5-dimethoxy-4-hydroxy-benzoic acid-7-O-beta-D-glucoside (1), myricananin F (2), beta-sitosterol (3), oleanolic acid (4), walnut ketone (5), asiatic acid (6), hederagenin (7), beta-carotene (8), (4R)-4,8-dihydroxy-alpha-tetralone-4-O-beta-D-glucopyranoside (9), 3beta,23-dihydroxy-12-olefin-28-ursolic acid (10), ursolicacid (11), syringic acid (12) and Juglanoside B (13). After screening of anti-tumor activity in vitro, it is found that some compounds have certain growth inhibitory activity against cancer cells andcan be further used for preparing anticancer drugs.

Description

technical field [0001] The invention belongs to the technical field of extraction, separation and purification of chemical components of walnut green husk, and in particular relates to a method for extraction, separation and purification of chemical components of walnut green husk and an application thereof. Background technique [0002] Walnut green peel, also known as Qinglongyi, is the immature peel of Juglans walnut (Juglans regia L.) and walnut catalpa (J. mandshurica Maxim.). my country is rich in walnut resources, which are distributed in Hebei, Shandong, Northeast and other provinces except Henan. Walnut green bark is pungent, bitter, astringent, poisonous, and slightly cold. It has the functions of clearing away heat, detoxifying, treating dysentery, improving eyesight, and anti-tumor, so it has great medical application value. But so far, domestic and foreign scholars have not done much systematic research on the active ingredients of walnut green peel, and they o...

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Application Information

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IPC IPC(8): C07H15/203C07H1/08C07J9/00C07J63/00C07C49/747C07C45/79C07J17/00C07C65/21C07C51/47A61P35/00
Inventor 殷田田闫福林李彦灵马矜烁詹璐璐吴亦男石伟峰
Owner 新乡医学院三全学院
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