Method for extracting, separating and purifying chemical components of walnut green seedcases and application of chemical components
A technology of walnut green husk and purification method, applied in the separation/purification of carboxylic acid compounds, separation/purification of carbonyl compounds, chemical instruments and methods, etc., can solve the problems of no research reports, etc., and achieve the effect of strong growth inhibitory activity
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[0023]In order to further understand the chemical constituents of walnut green peels from different origins and explore their medicinal value, this experiment studied the chemical constituents of walnut green peels collected from Checun Town, Songxian County, Luoyang City, Henan Province, and isolated 13 compounds, which were identified as : 3,5-dimethoxy-4-hydroxy-benzoic acid-7-O-β-D-glucoside (1), myricananin F (2), β-sitosterol (3), oleanolic acid ( 4), walnut ketone (5), Asiatic acid (6), hedera saponin (7), β-carotin (8), (4R)-4,8-dihydroxy-α-tetralone- 4-O-β-D-glucopyranoside (9), 3β,23-dihydroxy-12-ene-28-ursolic acid (10), ursolic acid (11), syringic acid (12), Juglanoside B(13). Among them, compounds 1, 2, 6, 7, 9, and 10 were isolated from this plant for the first time, and the six compounds were also isolated from this genus for the first time.
[0024] Using MTT staining method, compounds 1~6, 9, 11 and 13 were respectively mixed with human liver cancer cells (H...
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