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4918results about How to "Keep alive" patented technology

Liquid nitrogen cylinder, cryogenic vial storing and fetching device and liquid nitrogen cylinder storing and fetching system

The invention discloses a liquid nitrogen cylinder, a cryogenic vial storing and fetching device and a liquid nitrogen cylinder storing and fetching system. The liquid nitrogen cylinder comprises a cylinder body, wherein the cylinder body is provided with a sealed opening; the liquid nitrogen cylinder further comprises a bracket tray; the bracket tray is arranged in the cylinder body; the bracket tray is used for bearing cryogenic baskets; a central shaft is vertical to the bracket tray and is fixedly connected with the bracket tray; a driving component is electrically connected with the central shaft; the driving component is used for driving the central shaft to rotate; and the central shaft is used for driving the cryogenic baskets to rotate through the bracket tray and conveying the cryogenic baskets to the opening. By adopting the liquid nitrogen cylinder, the error rate of manual cryogenic vial selecting is reduced; and meanwhile, safety hazards for operation personnel during a manual bracket tray rotation process are avoided. By adopting the cryogenic vial storing and fetching device, the process for selecting a single target cryogenic vial for biological samples is realized; and during the whole selecting process, the cryogenic vials are all in a liquid nitrogen environment, so that non-target cryogenic vials are prevented from being exposed at environment temperature. The liquid nitrogen cylinder storing and fetching system is applicable to vial picking work for large biological sample banks.
Owner:SHANGHAI ORIGINCELL BIOLOGICAL CRYO EQUIP CO LTD

Microporous-mesoporous molecular sieve containing noble metal, preparation method and application to catalytic reduction of p-nitrophenol

The invention belongs to the technical field of molecular sieve preparation, and particularly relates to an in-situ preparation method for a microporous-mesoporous molecular sieve containing noble metal. The in-situ preparation method comprises the steps as follows: adding a coupling pore-forming agent, a silicon source, an aluminum source or a titanium source, and an alkali source into a water solution of noble metal nano particles in sequence under the water bath condition; and ageing, drying, crystallizing, drying and carrying out high-temperature calcination to obtain the microporous-mesoporous molecular sieve containing the noble metal. The prepared microporous-mesoporous molecular sieve is provided with a hierarchical pore structure; the noble metal nano particles with high dispersity are covered in situ while a mesoporous structure is generated; and a synthetic method is convenient and simple, saves energy and reduces emission. A multifunctional catalyst prepared with the method integrates the advantages of the microporous channels of the molecular sieve, the transgranular meso pores and the intergranular meso pores of the molecular sieve and the noble metal nano particles, and is more suitable for catalytic reactions of sulfur-containing large molecules such as hydrogen desulfurization and the like.
Owner:JILIN UNIV

Silicon-based composite material and preparation method and applications thereof

ActiveCN103400971AGuaranteed lattice structureKeep aliveCell electrodesHigh temperature storageAlcohol
The invention provides a silicon-based composite material, which is prepared from silicon particles, silicate and optional carbon, wherein the mixture of the silicate and the optional carbon forms a massive body, and the silicon particles are dispersed in the massive body. A preparation method comprises the following steps of: dispersing the silicon particles into absolute ethyl alcohol and/or deionized water to form suspension liquid; dispersing the silicate and the optional carbon into the absolute ethyl alcohol and/or the deionized water to form suspension liquid; ultrasonically oscillating the two kinds of suspension liquid respectively, and then stirring; dropwise adding the suspension liquid of the silicon particles into the suspension liquid of the silicate and the optional carbon to form mixed liquid, heating and stirring the mixed liquid until evaporating the mixed liquid into paste; then, putting the paste in an oven to be dried to obtain masses, and grinding and sieving to obtain undersize particles; and conducting heat treatment in an inert atmosphere, and grinding and sieving to obtain the silicon-based composite material. According to the silicon-based composite material, a lattice structure of the silicon particles can be ensured, therefore the activity of the silicon particles is ensured, and the energy density, the first-time coulomb efficiency and the high-temperature storage performance of lithium ion batteries are increased.
Owner:NINGDE AMPEREX TECH

Standby electrical power system of fuel cell for communication

The invention relates to a communication fuel cell spare power supply system, which comprises a hydrogen generation and storage unit, a fuel cell unit, a DC/DC unit, an output unit, an electric control unit, a routing inspection unit, a monitoring unit and a communication unit, and is characterized in that: the hydrogen generation and storage unit generates hydrogen by using solar or wind energy and releases the hydrogen by absorbing the heat of the fuel cell unit; the fuel cell unit generates direct current electric energy and heat by the electrochemical reaction of the hydrogen and oxygen; the DC/DC unit adjusts and boosts the direct current electric energy and then supplies the direct current electric energy to the output unit; the output unit supplies power to a load when commercial power fails; the electric control unit acquires various data and sends control information to all units; the routing inspection unit acquires all individual cell voltage values for transmission; the monitoring unit displays various parameters and working states and realizes human-computer interaction; and the communication unit performs short-range and long-range communication and monitoring. The power supply system is clean, high efficient, reliable and applicable to various communication spare power supplies.
Owner:武汉海亿新能源科技有限公司

Liquid nitrogen container and cryogenic vial pick-and-place device

The invention discloses a liquid nitrogen container and a cryogenic vial pick-and-place device. The liquid nitrogen container comprises a container body, and further comprises a rotary disc, a cryopreservation frame, a central shaft and a driving assembly, wherein an opening is formed in the container body; the rotary disc is arranged in the container body; the cryopreservation frame is supported by the rotary disc and comprises a plurality of layers of placement faces; the included angle between each placement face layer and the rotary disc is 40-60 degrees; the central shaft is perpendicularly arranged with and fixedly connected with the rotary disc; the driving assembly is electrically connected with the central shaft and used for driving the central shaft to rotate; and the central shaft is used for driving the cryopreservation frame to rotate through the rotary disc to reach the opening of the container body. By adoption of the liquid nitrogen container and the cryogenic vial pick-and-place device, the workload of operating personnel is reduced, the error rate of manual picking of cryogenic vials is lowered, and the potential safety hazard for the operating personnel is avoided. The liquid nitrogen container and the cryogenic vial pick-and-place device are particularly suitable for the work of picking cryogenic vials in a high-capacity biological sample bank, and meet the technical requirement for storage in the high-capacity biological sample bank.
Owner:SHANGHAI ORIGINCELL BIOLOGICAL CRYO EQUIP CO LTD

Drug delivery system and preparation method thereof

The invention belongs to the technical field of medicines, and discloses a delivery system based on protein-phospholipid. The system comprises a protein-phospholipid dispersion with the weight ratio of between 1:100 and 100:1, wherein the grain size of the protein-phospholipid is between 5 and 1,000nm, and the surface of the phospholipid or the surface and the inside of the phospholipid are coated / covered by a protein layer. The preparation method comprises: preparing a phospholipid containing phase, preparing a protein containing water phase, performing the homogenization treatment on the phospholipid containing phase and the protein containing water phase at a temperature of between 0 and 40 DEG C and under a pressure of between 400 and 40,000psi, and obtaining a dispersion system with the average grain size of less than 1,000nm. The delivery system has the advantages of realizing the delivery in various ways such as injection, oral taking, cavities and canals, skin and the like, and lowering side effects of medicines. The delivery system solves the problems of drug-loading rate and stability of protein nano-grain and liposome, and adopts low-toxicity organic solvent, even no organic solvent; and the preparation method is easy for industrialized application, and can prepare a liquid type protein-phospholipid containing delivery system.
Owner:SHENYANG PHARMA UNIVERSITY

Preparation method of high-purity collagen protein sponge

The invention provides a preparation method of high-purity collagen protein sponge, and relates to a preparation method of collagen protein sponge. The preparation method of the high-purity collagen protein sponge is used for solving the problems that the collagen protein sponge prepared by using a conventional method is long in production cycle and low in yield and purity and has poor hemostasis performance. The preparation method of the high-purity collagen protein sponge comprises the following steps: step one. pretreating fresh bovine heel tendons; step two. extracting collagen protein; step three. centrifuging; step four. salting out; step five. dissolving; step six. carrying out gradient dialysis; step seven. pre-freezing; step eight. carrying out freeze drying; and step nine. sterilizing. The final product prepared by using the method has a smooth and flat surface and relatively good hemostatic performance and is uniform in pore size distribution. The product has relatively high purity (the total amount of amino acids reaches 97.73%), an obvious hemostatic effect and no abnormal taste, is safe, non-toxic, high in yield and short in time; liquid is clear without impurities; the production cycle is shortened; the whole preparation process is carried out at a room temperature; the biological activity of the collagen protein is maintained; and the application of the high-purity collagen protein sponge in clinical is improved.
Owner:HARBIN INST OF TECH

Method for preparing exosome freeze-dried powder of human amniotic mesenchymal stem cells

The invention relates to the field of stem cells, discloses a method for preparing exosome freeze-dried powder of stem cells, and particularly relates to a method for preparing exosome freeze-dried powder of human amniotic mesenchymal stem cells. The method for preparing the exosome freeze-dried powder of the human amniotic mesenchymal stem cells disclosed by the invention comprises the following steps: mixing trehalose with exosomes of the human amniotic mesenchymal stem cells as a cryoprotectant; filtering and removing bacteria by virtue of a filter membrane; and firstly carrying out ultralow temperature pre-freezing, and then carrying out low-temperature freezing at a certain vacuum degree. The preparation method disclosed by the invention is simple to operate, safe and effective. An experiment proves that compared with a conventional preparation method of the freeze-dried powder, the morphologies and the activity of the exosomes of the human amniotic mesenchymal stem cells can be well kept by the method for preparing the exosome freeze-dried powder of the human amniotic mesenchymal stem cells disclosed by the invention; and the method is suitable for long-term preservation and application of the exosomes of the human amniotic mesenchymal stem cells.
Owner:GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD

Continuous production method of epoxy chloropropane by hydrogen peroxide process

The invention provides a preparation method of chloroepoxy propane by chloropropene epoxidation. Chloropropene, a solvent and titanium silicalite molecular sieve catalyst fine particles are put in a reaction kettle for reaction at the temperature of 10-80 DEG C. The method is characterized in that the reaction kettle is a single kettle or multistage slurry bed reaction kettles with same or different volumes which are connected in series; the reaction solution needs not to be homogeneous phase, slurry particles of the chloropropene, the solvent and the titanium silicalite molecular sieve catalyst are added to a first stage reaction kettle by a metering pump, hydrogen peroxide is dropwise added to the first stage reaction kettle or each stage of the reaction kettles, the reaction occurs in a weakly acidic condition, materials are subject to oil-water delamination after the reaction to be divided into oil-phase slurry and water-phase slurry; the catalyst is suspended in the water phase, and the oil phase is sent to a rectification tower to separate the chloropropene, the epichlorohydrin and trace methanol; the water phase slurry is filtered, a catalyst filter cake or concentrated slurry is directly beaten by the solvent, and pumped to a first stage epoxidation reaction kettle, the water phase slurry contains the methanol, trace epichlorohydrin and the water which is generated by the reaction and the water which is taken by the hydrogen peroxide, the trace methanol and the epichlorohydrin are separated out by negative pressure rectification, and the recovered methanol is used for preparation of the catalyst slurry. The method has the advantages of simple process, little solvent consumption and high product separation yield, and is available for industrialized production.
Owner:JIANGSU YANGNONG CHEM GROUP +2

Probiotic ultralow temperature refrigeration technology and applications thereof in probiotic preparation

The invention discloses a probiotic ultralow temperature refrigeration technology, a formula of a total-salt cryoprotectant which is suitable for the probiotic ultralow temperature refrigeration technology, and probiotic ultralow temperature refrigeration particles prepared by using the probiotic ultralow temperature refrigeration technology. The probiotic ultralow temperature refrigeration technology comprises following steps: (1) preparing a fermentation medium; (2) culturing probiotics; (3) centrifuging; (4) preparing the cryoprotectant; (5) adding the cryoprotectant; and (6) quick-freezing and moulding. The invention also provides a probiotic preparation prepared by using the probiotic ultralow temperature refrigeration particles, and a preparation method of the probiotic preparation. The probiotic ultralow temperature refrigeration particles are probiotic refrigerated bacterial strains which do not need to be dried and possesses advantages of fast refrigeration speed, high living bacterial rate and excellent rehydration performance. The probiotic ultralow temperature refrigeration particles contain water, can be dissolved in solutions more quickly, and are capable of preventing probiotic cells from damage caused by rehydration of normal low temperature refrigeration dried probiotic powder, and increasing the living bacterial rate of the probiotic preparation greatly.
Owner:THANKCOME BIOLOGICAL SCI & TECH CO LTD

Face verification anti-counterfeit recognition method and system thereof based on interactive action

The invention provides a face verification anti-counterfeit recognition method and a system thereof based on an interactive action. The method comprises a step of carrying out the initial recording of the information of an register static face image and the information multiple register face action images, a step of waiting the reading of a static face image to be read, matching a character and a stored character when the shooting of static face image to be detected obtained by a user to be verified is detected, and if a matching degree reaches the storage characteristic of a preset threshold value, conforming to a verification requirement, and a step of randomly selecting and prompting the user to be verified to complete a corresponding face action according to the recorded face action, extracting the characteristic of the action image to be detected of the user be verified, matching the characteristic with the historical verification feature information of a corresponding face action, completing face identity verification if a matching rate reaches a preset threshold value, adding the matched image into the historical verification feature information, returning to select a next face action to continue matching if the matching is not approved or does not reaches a desired effect, treating the verification as a failure if the action exceeds a preset number of times, and ending the verification.
Owner:HUBEI UNIV OF ARTS & SCI

Byzantine fault-tolerant alliance chain consensus method and system for power consumption information protection and storage medium

The invention discloses a Byzantine fault-tolerant alliance chain consensus method and system for power consumption information protection and a storage medium, and the method comprises the steps thata blockchain node credit model is built, and a node with the highest credit is selected as a main node through the blockchain node credit model; the transaction initiator broadcasts transactions andpackages the transaction requests into blocks; the main node generates a proposal message from the block and signs the proposal message; after the slave node receives the proposal information, the legality of the proposal is verified, when the verification result is illegal, recording is carried out, the consensus is given up, and a view replacement protocol is initiated; the verified slave node enters a confirmation stage, executes the block transaction and replies a message to the client; and consensus is reached according to the node reply message condition received by the client, and eachnode executes block chaining. According to the method, the probability that the Byzantine node is the main node is reduced, so that the view replacement frequency is greatly reduced, and the system performance is effectively improved.
Owner:STATE GRID JIANGSU ELECTRIC POWER CO ELECTRIC POWER RES INST +3

Preparation method of shell core fibre tectorial membrana endovascular stent

The invention relates to a preparation method of a shell core fiber covered endovascular stent graft. The steps are as follows: firstly, a polymer is dissolved in adaptive dissolvent to obtain solution with a certain concentration; secondly, medicine or an artificial polymer and medicine, and a bio-active element are dissolved in the adaptive dissolvent to obtain solution or suspension liquid; thirdly, the solution of the polymer and the medicine or the solution or suspension liquid of the medicine or bio-active element are respectively filled in two injectors, the speed of a micro injection pump, the voltage of an electrostatic generator and the distance of a receiving device are adjusted, fiber is obtained through the static spinning preparation, and the fiber is received as a tube shape or film shape structure; fourthly, the endovascular stent graft is fixed on a revolution axle, through the revolution of the endovascular stent graft, the static spinning fiber are directly received as fibrous membrane covered on the endovascular stent graft. The invention can effectively prevent smooth muscle cells from hyperplasia in the stent graft or from narrowing in the stent graft caused by the other functions, and the shell core fiber for the medicine loading can slowly release the medicine to attain the purpose of curing.
Owner:DONGHUA UNIV

Cell cryopreservation liquid, application thereof and cryopreservation method of megakaryocyte progenitor cells

The invention relates to the field of cell culture, in particular to cell cryopreservation liquid, application thereof and a cryopreservation method of megakaryocyte progenitor cells. The cell cryopreservation liquid comprises DMSO, fetal calf serum, dextran, trehalose and albumin. By means of the cell cryopreservation liquid, the cytoactive of the megakaryocyte progenitor cells can be well maintained during the cryopreservation period, and damage to cells in the cryopreservation and resuscitation processes is lowered. According to the cell cryopreservation liquid, application thereof and the cryopreservation method of the megakaryocyte progenitor cells, the cryopreservation and temperature reduction processes are softer, the cells are stored in liquid nitrogen after the temperature reduction process is conducted, the influence on the cytoactive is small, and damage to the cells in the cryopreservation process is lowered. It is indicated by experiments that the vigour of the resuscitated cells can reach 94% one month after the megakaryocyte progenitor cells are cryopreservated in the cryopreservation liquid, and multiplication activity is good, and the cell cryopreservation liquid is significantly superior to the prior art.
Owner:GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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