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399results about How to "Keep alive" patented technology

Ventilation device for green building

The invention discloses a ventilation and air exchange device for a green building. The ventilation and air exchange device comprises a ventilation and air exchange box, a mounting plate, a ventilation and air exchange opening, a rotary air disc and fan blades. Mounting plates are fixed to the two ends of the two sides of the ventilation box, fixing pins penetrate through the interiors of the mounting plates, one ends of the fixing pins extend into the green building wall, and ventilation ports are formed in the two ends of the outer side of the ventilation box. According to the ventilation device for the green building, a fan motor is used for driving a linkage shaft to rotate, and a transmission assembly is matched to enable a fan shaft to rotate synchronously, so that two rotating wind discs and a plurality of fan blades are driven to rotate, outdoor fresh air is extracted and fed into the green building, and the ventilation effect is improved. According to the indoor air purification device, vitiated air, harmful gas and microorganisms in a building can be effectively diluted and discharged, so that the indoor air quality is improved, a healthier and more comfortable living environment is provided for people, indoor oxygen can be effectively supplemented, and the freshness and vitality of indoor air are kept.
Owner:ANQIU XINGLONG CONSTR & INSTALLATION CO LTD

High-stability immobilized candida antarctica lipase B as well as preparation method and application thereof

The invention discloses high-stability immobilized candida antarctica lipase B as well as a preparation method and application thereof, and belongs to the technical field of enzyme engineering and biological catalysis. The preparation method comprises the following steps: modifying resin by adopting a silane coupling agent to obtain silanized resin; carrying out aldehyde functional modification on the silanized resin by using dialdehyde cellulose to obtain alkyl-aldehyde modified resin; candida antarctica lipase B is immobilized by using alkyl-aldehyde modified resin, and after the Candida antarctica lipase B is subjected to interfacial activation on the surface of the alkyl-aldehyde modified resin, the Candida antarctica lipase B is covalently bound with aldehyde on the surface of the alkyl-aldehyde modified resin; the prepared immobilized Candida antarctica lipase B has relatively high activity and stability. The method is simple to operate, the prepared immobilized lipase is high in stability, and the method can be used for preparing the high-stability immobilized lipase on a large scale and has the potential of industrial popularization and application.
Owner:SHAANXI UNIV OF SCI & TECH

PH-responsive chitosan medical biological adhesive as well as preparation and application methods thereof

The invention relates to the technical field of biological adhesives, in particular to a pH response type chitosan medical biological adhesive and a preparation and application method thereof.The preparation method includes the steps that phenylboronic acid modified chitosan, polyphenol compounds, cross-linking agents with different functional groups and polyethylene glycol with different molecular weights are evenly mixed in a PBS buffer solution with the pH equal to 7-8; and adjusting the pH value of the system to 7.5-8 by using a KOH aqueous solution, and standing at room temperature until the pH-responsive chitosan medical biological adhesive is formed. The chitosan biological adhesive disclosed by the invention still keeps excellent adhesive power in a moist environment, is rapid in adhesion, is non-toxic and biodegradable in material, has good biocompatibility, and effectively avoids secondary stitch removal pain caused by non-degradable materials. Due to the charge characteristic of the chitosan, the chitosan has good affinity with human tissues, is suitable for adhesion of various tissues, and has wide clinical application potential.
Owner:JIANGSU TECH-BIO-MED MEDICAL EQUIP CO LTD

Liquid storage type micro-fluidic chip and working method thereof

The invention discloses a liquid storage type micro-fluidic chip and a working method thereof. The chip comprises a chip body, a sealing film and gum, the sealing film and the back adhesive are respectively bonded on the front surface and the back surface of the chip body; a plurality of solution storage cavities are formed in the chip body, outflow runners at the bottoms of the solution storage cavities are connected with corresponding first runners, sealing partition pieces are arranged on the first runners, the outflow runners and the corresponding first runners are blocked through the sealing partition pieces, and the first runners are communicated with second runners through corresponding gum runners. The whole experiment process does not need to be externally connected with a reagent bottle, the purposes of instant detection, preparation and sample separation of the micro-fluidic chip can be achieved, the detection time is greatly shortened, and the experiment steps are simplified. Waste liquid generated in the experiment process flows into the waste liquid cavity, and cross contamination of a laboratory is avoided. The chip provided by the invention can be used for identifying the species of pathogenic microorganisms.
Owner:ZHIMEI TIMES BIOLOGICAL INTELLIGENT TECH (BEIJING) CO LTD +1

Hydrolysis acidification wastewater treatment process for strengthening extracellular electron transfer

The invention relates to the technical field of wastewater treatment, in particular to a hydrolytic acidification wastewater treatment process for strengthening extracellular electron transfer, which comprises the following steps: S1, constructing a hydrolytic acidification reactor, taking carbon fiber suspension filler as an anode, taking a steel wire mesh frame as a cathode, connecting the carbon fiber suspension filler in series by a stainless steel wire rope, and taking the stainless steel wire rope as a cathode; the carbon fiber suspension filler is connected with the steel wire mesh frame through an electrochemical workstation; s2, inoculating hydrolytic acidification bacteria into a reactor, controlling culture conditions, and domesticating microorganisms in a micro-current environment until a biological membrane is formed on the carbon fiber suspension filler; s3, inputting wastewater to be treated into the reactor, controlling treatment conditions, monitoring an effluent result, and discharging after the effluent result reaches the standard. Compared with traditional interspecific electron transfer, the wastewater treatment method has higher efficiency and speed, the oxygenolysis process of organic matter is accelerated, and the overall efficiency of wastewater treatment is improved.
Owner:HEFEI UNIV OF TECH +1

Method for extracting amide components in Chinese prickly ash by using superfine grinding assisted natural eutectic solvent

The invention belongs to the field of comprehensive utilization of plant resources, and relates to a method for extracting amide components in Chinese prickly ash through a natural eutectic solvent assisted by superfine grinding. The method comprises the following steps: firstly, collecting a pepper raw material, drying the pepper raw material by a heat pump, and performing high-speed crushing and low-temperature superfine crushing to obtain pepper superfine powder; preparing a natural eutectic solvent from a hydrogen bond acceptor and a hydrogen bond donor, mixing the natural eutectic solvent with the pepper superfine powder, performing microwave-assisted extraction, centrifugally collecting supernate after extraction, and completing primary extraction; adding NDES into the remaining precipitate, and repeatedly extracting according to the primary extraction step; and repeatedly extracting for several times, combining the supernate, and finally concentrating and filtering with a filter membrane to obtain an extracting solution, thereby realizing the extraction of the amide components in the Chinese prickly ash. The method provided by the invention overcomes the defects of low extraction efficiency, environmental pollution caused by an extraction reagent, high equipment cost and the like in the existing technical means for extracting the amide components in the Chinese prickly ash, realizes efficient and green extraction of the amide components in the Chinese prickly ash, and has a wide application prospect.
Owner:NANJING INST FOR THE COMPREHENSIVE UTILIZATION OF WILD PLANTS CHINA COOP +1

Green low-carbon biomass coupling fused salt energy storage methanol preparation system

ActiveCN119462336Areduce usageLower Disposal CostsElectrolysis componentsOrganic compound preparationResidual biomassNew energy
The invention provides a green low-carbon biomass coupling fused salt energy storage methanol preparation system, and belongs to the field of methanol preparation systems. The problem that methanol purge gas is released and residual biomass gas is generated and needs to be treated in the process of preparing methanol through biomass gasification is solved. One part of new energy electricity is used for electrolyzing water, the other part of new energy electricity serves as abandoned electricity to enter a fused salt electric heater, hydrogen generated by electrolyzing water enters a catalytic synthesis tower, oxygen is used for biomass gasification, generated carbon monoxide, hydrogen and carbon dioxide enter the catalytic synthesis tower, and methanol is synthesized in the catalytic synthesis tower. Biomass, redundant biomass gas and formaldehyde purge gas enter the high-temperature and high-pressure biomass fluidized bed boiler, then superheated steam generated by the high-temperature and high-pressure biomass fluidized bed boiler and part of steam serve as a catalytic temperature heat source for methanol production, the remaining steam enters the power generation system to be used for power generation, and stable power supply of the whole system is guaranteed. And the generated high-temperature and high-pressure steam is used for power generation to improve the unit efficiency.
Owner:HARBIN BOILER CO LTD +1

Single cell capturing method and micropore coupling micro-cavity micro-fluidic chip

The invention relates to a single cell capturing method, which comprises the following steps that a single cell to be captured is injected into a micro-fluidic chip containing a micropore coupling micro-cavity through fluid, the chip comprises a base material and a main channel arranged on the base material, the surface of the main channel is provided with a plurality of micropores and micro-cavities corresponding to the micropores, and the micro-cavities are communicated with the main channel; after the main channel is filled with the fluid, the fluid in the main channel is kept static, so that the single cells fall into the micropores under the action of gravity; after the single cells are captured by the micropores, the fluid is introduced again to discharge the uncaptured single cells; and the chip is overturned and inclined after being discharged, so that the single cells enter the micro-cavity. The technical problems that in the prior art, single cell capture efficiency is low, capture precision is insufficient, complex external equipment is needed for control, and single cell capture, culture and the like cannot be completed in the same chip are solved.
Owner:QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI

Roof waterproof protection layer construction method based on microbial concrete

The invention belongs to the field of roof waterproof protection layers, and particularly discloses a roof waterproof protection layer construction method based on microbial concrete, which comprises the following specific steps: S1, microbial culture; s2, protective carrier wrapping; s3, preparing concrete; s4, concrete pouring; s5, concrete curing; according to the invention, expanded perlite particles are used as a carrier medium to respectively immobilize individual bacillus and aerobic mixed bacteria, so that the expanded perlite particles are activated and induced to form a composite mineralized structure when cracks are formed, the crack repairing effect is enhanced, and the high strength of aragonite and the good filling property of calcite are combined, so that the crack repairing effect is improved. According to the present invention, the expanded perlite particles are adopted as the carrier medium, and are combined with the hydrogel wrapping the surface of the bacteria-containing expanded perlite, such that the microorganism can be protected, the water storage agent can be used as the water storage agent, and the living environment of the microorganism can be enhanced.
Owner:CHINA MCC17 GRP CO LTD

Low-irritation essential oil hair dye and preparation method thereof

ActiveCN119405565Adesensitizationless discomfortCosmetic preparationsHair cosmeticsHair ColorantsStearyltrimethylammonium chloride
The invention relates to the field of hair dyeing products, in particular to a low-irritation essential oil hair dye and a preparation method thereof. The invention relates to a low-irritation essential oil hair dye, which comprises a No.1 agent and a No.2 agent, the No.1 agent comprises a humectant, a hair coloring agent, an antioxidant, a chelating agent and a solvent A; a thickening agent A, an emulsifier A and a hair conditioner; a pH regulator A, and polydimethylsiloxane; the disinfectant is prepared from an aromatic A, polyquaternium-7, polyquaternium-10, argania spinosa kernel oil, a camellia extract, a ginger root extract and a preservative; the agent 2 comprises a solvent B, cetearyl trimethyl ammonium chloride, sodium stannate and a pH regulator B; a thickener B, an emulsifier B and mineral oil; a chelating agent C; and an oxidant. The hair dye provided by the invention can reduce irritation under the condition of ensuring the hair dyeing effect and relieve the frizz degree of dyed hair, and has a plurality of advantages.
Owner:VENUS SKIN & HAIR LAB LTD +1

Hydrophilic carbon-based material modified high-entropy alloy water electrolysis hydrogen production catalyst as well as preparation method and application thereof

The invention discloses a hydrophilic carbon-based material modified high-entropy alloy water electrolysis hydrogen production catalyst and a preparation method and application thereof, and belongs to the technical field of catalyst preparation. Comprising the following steps: 1) adding a hydrophilic carbon-based material and mixed metal salt into an ethylene glycol solution, and ultrasonically mixing uniformly to obtain a reaction solution; and 2) heating an ethylene glycol solution to 200-240 DEG C through an oil bath, then slowly adding the reaction liquid prepared in the step 1) into the ethylene glycol solution, carrying out heating treatment for 0.5-2 hours, cooling, and centrifuging to prepare the carbon-based material modified high-entropy alloy water electrolysis hydrogen production catalyst. The prepared hydrophilic carbon-based material modified high-entropy alloy water electrolysis hydrogen production catalyst has high activity, and generated hydrogen bubbles are smaller and can be separated from the surface of an electrode more easily.
Owner:XI AN JIAOTONG UNIV

Fermented feed for improving intestinal health and preparation method thereof

The invention discloses a fermented feed for improving intestinal health and a preparation method thereof, and relates to the technical field of feed processing; the fermented feed for improving intestinal health consists of ZIF‑8@Clove Basil Oil Nanoparticles, Laver Polysaccharide Active Traditional Chinese Medicine Composite Gel, corn flour, soybean meal powder, grass powder, Bacillus subtilis, yeast and papain; the Laver Polysaccharide Active Traditional Chinese Medicine Composite Gel consists of Laver Polysaccharide, chitosan, compound traditional Chinese medicine superfine powder and citric acid; the ZIF‑8@Clove Basil Oil Nanoparticles are combined with the Laver Polysaccharide Active Traditional Chinese Medicine Composite Gel, and then coated with the fermented feed, so as to effectively isolate the feed from the external environment, maintain the activity of microorganisms in the fermented feed, increase the stability of the feed, and can also absorb harmful substances in the feed, reduce the adverse effects on the digestive tract, significantly improve intestinal health, thereby promoting digestion and absorption, and helping to improve the growth performance of animals.
Owner:CHENGDU DACANG AGRICULTURAL TECHNOLOGY CO LTD

Municipal sewage treatment device

The invention discloses a municipal sewage treatment device, and relates to the technical field of sewage treatment, the municipal sewage treatment device comprises a supporting assembly, the supporting assembly comprises an aeration box, a water outlet pipe and a water inlet pipe, and the water outlet pipe and the water inlet pipe are fixed on the aeration box; the device further comprises a gas transmission assembly and a fermentation assembly; the fermentation assembly comprises a fermentation box; the fermentation box is located at the bottom of the aeration box; the gas transmission assembly comprises a vortex tube, a connecting tube, a nozzle, a gas transmission tube and a driving pump; the driving pump is fixed on the side wall of the fermentation box, the driving pump is fixedly connected with an air inlet of the vortex tube, and the driving pump conveys hot air in the fermentation box into the connecting tube through the vortex tube; the connecting pipe is located in the aeration box, and a plurality of gas conveying pipes are fixedly connected to the connecting pipe and located at the bottom of the aeration box; the multiple nozzles are fixedly connected to the air conveying pipe. The temperature in the sewage treatment tank can be increased to a range suitable for microbial metabolism and reproduction when the temperature is too low, the degradation speed of microorganisms on organic matters is increased, and the effluent quality of the sewage treatment tank is ensured.
Owner:XUZHOU LITIAN ENVIRONMENTAL PROTECTION TECH RES INST CO LTD

Preparation process and application of magnolol-loaded plant exosome

The invention discloses a preparation process and application of magnolol-loaded plant exosomes, and relates to the technical field of plant exosomes. Magnolol is extracted from a magnolia officinalis plant material through ultrasonic assistance, the plant exosomes are obtained from fresh ginger by using an ultracentrifugation method, and the plant exosomes are further modified through palmitic acid and polyethylene glycol, so that the magnolol-loaded plant exosomes are obtained. And finally, preparing the magnolol-loaded plant exosome by virtue of an incubation method. The magnolol can be protected from being damaged by gastric acid and digestive enzymes through membrane structure wrapping of the plant exosome, the stability of the magnolol in the digestive tract is improved, and it is further verified through mouse experiments that the magnolol-loaded plant exosome can be beneficial to regulation of intestinal flora.
Owner:GUANGZHOU HONSEA SUNSHINE BIOTECH CO LTD

Production process of black ginseng peptide powder

The invention belongs to the technical field of health care products, and particularly relates to a production process of black ginseng peptide powder, and the technical scheme is that the production process of the black ginseng peptide powder comprises the following steps: pre-treating raw materials, cleaning black ginseng, removing impurities, ensuring the purity of the raw materials, cooking the cleaned black ginseng, and drying to obtain the black ginseng peptide powder. The method comprises the following steps: extracting active ingredients in black ginseng, adding a mixed enzyme for enzymolysis, decomposing proteins in the black ginseng into small-molecular peptides under the action of the mixed enzyme to obtain a liquid after enzymolysis, carrying out precise filtration, carrying out precise filtration on the liquid after enzymolysis, removing impurities to obtain a pure peptide liquid, and concentrating the pure peptide liquid by using concentration equipment to obtain the black ginseng peptide. The size is reduced; in the enzymolysis step of the production process of the black ginseng peptide powder, protein in black ginseng can be decomposed more comprehensively by using mixed enzyme and is converted into small molecule peptide, and the small molecule peptide is more easily absorbed and utilized by a human body.
Owner:FUSONG COUNTY CHUANQI ECOLOGY GINSENG IND CO LTD

Liver function biochemical composite quality control product as well as preparation method and application thereof

The invention discloses a liver function biochemical composite quality control product as well as a preparation method and application thereof. The quality control product comprises 12 analyte raw materials and the following non-analyte raw materials: 5 to 12 g / L of sodium chloride, 0.5 to 2 g / L of casein sodium salt, 5 to 20 g / L of mannitol, 0.05 to 0.2 g / L of Tween 20, 1 to 20 mg / L of bright aprotinin, 10 to 60 g / L of trehalose and 0.05 to 0.2 g / L of PC300. And matrix liquid: 20-80% of quality control substance matrix liquid and 20-80% of buffer solution. The liver function biochemical composite quality control product disclosed by the invention has high compositing property, contains 11 important liver function biochemical indexes, is excellent in component uniformity and stability, ensures the precision of each index, and meets the requirements of daily quality control.
Owner:GENEWELL BIOTECHNOLOGY CO LTD

Test tube for sampling

The utility model discloses a test tube for sampling, which relates to the technical field of sampling, and comprises a test tube main body and a cover cap, the outer surface of the test tube main body is provided with an anti-slip sleeve, the anti-slip sleeve is internally provided with an anti-falling component, the anti-falling component comprises a support rod, the support rod is positioned in a groove of the anti-slip sleeve, and the cover cap is arranged on the support rod. The top of the support is provided with a take-up and pay-off barrel, one end of the take-up and pay-off barrel is provided with a hexagonal rod, a rope is installed in the take-up and pay-off barrel in a surrounding mode, one end of the take-up and pay-off barrel is provided with a movable groove, a lead screw is installed in the movable groove, and one end of the lead screw is provided with a driving motor. The anti-falling assembly is installed, the cap and the anti-skid sleeve are connected and fixed through the rope in the winding and unwinding barrel inside the anti-skid sleeve, and the length of the winding and unwinding rope of the winding and unwinding barrel is conveniently controlled while the movable protruding block is driven by the lead screw to move; and the rope is convenient to take and can prevent the cap from being lost and being stained with dust and bacteria when being placed on the ground.
Owner:HENAN TAIYU GAOFEN MEDICAL DEVICES CO LTD

Graphite roasting device

The invention discloses a graphite roasting device. Comprising a mounting mechanism, a roasting mechanism is mounted on the mounting mechanism, one end of the roasting mechanism is connected with a power mechanism, and a cover sealing mechanism is mounted at one end of the roasting mechanism; the mounting mechanism is used for supporting and mounting the roasting mechanism, the power mechanism is arranged at one end of the roasting mechanism and used for providing power output and driving the roasting mechanism to rotate, and the cover sealing mechanism is used for sealing the roasting mechanism so that graphite can be conveniently put into the roasting mechanism. The roasted graphite can be conveniently discharged; the graphite stirring device can provide speed reduction power to drive the roasting mechanism to rotate, graphite is overturned, the sealing performance and feeding and discharging are improved through the cover sealing mechanism, the roasting mechanism is supported and rotated through the mounting mechanism, the graphite is stably rotated and roasted, the graphite is stirred through rotation, the situation that the graphite adheres to the stirring device is reduced, and discharging and cleaning are facilitated.
Owner:YONGAN DINGFENG CARBON TECH CO LTD

High-flux and high-precision ceramic flat sheet membrane for removing iron fishy smell of drinking tap water and preparation method of high-flux and high-precision ceramic flat sheet membrane

The invention discloses a large-flux high-precision ceramic flat sheet membrane for removing iron and fishy smell of drinking tap water and a preparation method thereof, and belongs to the technical field of ceramic flat sheet membranes. The ceramic flat sheet membrane comprises a support body, a high-precision filter layer, an adsorption layer and a filling adsorbent. The preparation method comprises the following steps: 1) preparing a ceramic flat sheet membrane mud green body; (2) preparing a ceramic flat sheet membrane support body; 3) preparing a high-precision filter layer by a dip-coating method; (4) preparing an adsorption layer by a spraying method; and (5) filling a reinforced adsorbent and packaging an end. According to the present invention, the ultra-fine membrane material is used to produce the high separation precision, the effluent quality is ensured, the operation cost of the split type filtration is reduced, the molecular sieve adsorption membrane and the enhanced filling adsorbent are produced, and the iron ions in the urban tap water are effectively adsorbed; the vacuum dip-coating method is matched with the one-time sintering process, so that the working procedures can be saved, the yield is high, the membrane layer thickness and the membrane surface are uniform and consistent, basically no pore defect exists, and the flux and the precision stability of the ceramic flat membrane product are further stabilized.
Owner:JIANGSU PROVINCE YIXING NONMETALLIC CHEM MACHINERY FACTORY

Method for promoting proliferation and differentiation of cervical squamous epithelial cells

The invention belongs to the technical field of cell culture, and particularly relates to a method for promoting proliferation and differentiation of cervical squamous epithelial cells. The method comprises the following steps: S1, extracting and culturing primary cervix uteri squamous epithelial cells from cervix uteri tissues to obtain primary cervix uteri squamous epithelial cells; s2, adding the primary cervical squamous epithelial cells into a cell proliferation induction culture solution to induce cell proliferation, so as to obtain proliferated cervical squamous epithelial cells; and S3, uniformly mixing the proliferated cervical squamous epithelial cells with differentiation matrigel, and inducing cell differentiation by using a cell differentiation induction culture solution to obtain differentiated cervical squamous epithelial cells. Proliferation and differentiation of the cervical squamous epithelial cells are promoted by preparing a proper culture medium, and influence factors of proliferation and differentiation of the cervical squamous epithelial cells are researched by culturing the cervical squamous epithelial cells in vitro, so that the pathogenesis of cervical erosion and cervical cancer can be clarified; therefore, a direction is provided for research and development of medicines for treating cervical erosion and cervical cancer.
Owner:广州华熙奥颜生物科技有限公司

Resveratrol-loaded exosome as well as preparation method and application thereof

PendingCN119410577AIncrease load factorHigh drug loading rateCell dissociation methodsMetabolism disorderUltrafiltrationActive agent
The invention provides a resveratrol-loaded exosome and a preparation method thereof, and belongs to the technical field of biological medicine manufacturing. The preparation method of the resveratrol-loaded exosome comprises the following steps: performing 3D culture on human umbilical cord or hair follicle-derived mesenchymal stem cells under a low-oxygen condition, collecting supernate, and separating by a differential ultracentrifugation method to obtain the exosome of the mesenchymal stem cells; the method comprises the following steps: mixing a resveratrol solution containing a surfactant with the MSCs-Exos, and carrying out co-incubation and ultrafiltration to obtain the resveratrol loaded MSCs-Exos. According to the invention, the surfactant is added, so that not only are the entrapment rate and the drug loading rate of the MSCs-Exos loaded resveratrol greatly improved, but also the activity of the MSCs-Exos is maintained, the common effect of the MSCs-Exos and the resveratrol is fully exerted, and a synergistic effect is achieved. The resveratrol loaded MSCs-Exos disclosed by the invention has an effect of treating diabetes mellitus.
Owner:广州医科大学附属清远医院(清远市人民医院)

Process for preparing high-purity human blood coagulation factor IX from plasma

The invention provides a process for preparing a high-purity human blood coagulation factor IX from plasma. The process comprises the following steps: acquiring plasma; adding anion gel, adsorbing, and collecting an eluted protein solution; adding heparin sodium, filtering, ultra-filtering and dialyzing to obtain dialyzed protein liquid; adding an S / D solution; adding anion gel for secondary adsorption, and collecting eluted protein liquid II; performing filtration, ultrafiltration and dialysis, and collecting eluted protein liquid after dialysis; filtering is performed; carrying out affinity gel chromatography purification, collecting eluent, and carrying out ultrafiltration and dialysis; filtering, and adding a dialysate containing glycine for dialysis to obtain a human blood coagulation factor IX stock solution; adjusting the titer, and adding heparin sodium to obtain a diluted solution; pre-filtering the diluted liquid to obtain a semi-finished product of the human blood coagulation factor IX; sterilizing, filtering, sub-packaging, carrying out vacuum freeze drying, carrying out dry heat virus inactivation, inspecting and packaging. According to the invention, the production process is simplified, the cost is reduced, the activity of the finally obtained human blood coagulation factor IX is greater than 50IU / ml, and the specific activity is higher than 180IU / mg.
Owner:CHINA RESOURCES BOYA BIO-PHARM GRP CO LTD

In-vitro amplification culture method of NK (Natural Killer) cells

The invention belongs to the technical field of immune cell culture, and particularly relates to an in-vitro amplification culture method of NK cells. The amplification method comprises the steps of CD16 pretreatment, plasma inactivation, lymphocyte separation and multi-stage fluid infusion. The preparation method comprises the following steps: pretreating CD16 in a T75 culture flask, promoting attachment and activation of NK cells, simulating a natural activation state, improving proliferation efficiency, treating whole blood and inactivating plasma to eliminate immunosuppressive factors, reserving nutritional ingredients, supporting amplification of the NK cells, enriching the NK cells through a lymphocyte separating medium, removing impurities and improving purity, mixing an RPMI-1640 culture medium with a solution A, and culturing for 12-24 hours at a high temperature; oK432 and IL-15 are added, the microenvironment is optimized, growth of the NK cells is remarkably promoted, liquid supplementing operation and bottling and bagging are carried out for multiple times, sufficient space and nutrition are provided, the cells are gradually transferred into a serum-free culture medium, and the cell safety and clinical application potential are improved.
Owner:潍坊吉涛医学科技有限公司 +1

Micro-fluidic chip for sorting circulating tumor cells based on double-helix structure and sorting method of micro-fluidic chip

The invention discloses a micro-fluidic chip for sorting circulating tumor cells based on a double-helix structure and a sorting method thereof, the micro-fluidic chip has the double-helix structure comprising an inner outlet, and the micro-fluidic chip comprises a primary screening channel for primarily focusing and separating circulating tumor cells; the secondary screening channel is directly connected with the primary screening channel and is used for carrying out secondary focusing separation on the circulating tumor cells subjected to primary screening; wherein the primary screening channel is separated into two branches after rotating into the chip, one branch is connected with the middle outlet and used for discharging unfocused particles and redundant liquid, and the other branch is connected with the secondary screening channel so as to continue focusing and separation of the particles; the micro-fluidic chip is provided with an inlet for injecting a liquid sample and three outlets, namely a middle outlet, a particle collection outlet and a waste outlet, so that the injection of the liquid sample and the collection of separated particles are realized. According to the invention, high-throughput screening of the circulating tumor cells is realized, and the separation efficiency is improved.
Owner:DALIAN UNIV OF TECH

Electrolyte filling device for lithium battery production

The invention discloses an electrolyte filling device for lithium battery production, which comprises a shell, a stirring rod, a conveying block, a placing block, a conveying cylinder, a power device, a rotating assembly and a reciprocating assembly, when the electrolyte filling device is used, a battery is placed in a placing groove, a double-shaft motor drives a first driving shaft and a second driving shaft to rotate; the second driving shaft drives the rotating assembly to operate so as to drive the rotating shaft to intermittently rotate, the rotating shaft intermittently rotates to drive the batteries placed in the conveying groove to sequentially rotate to the bottom of the filling pipe, and the second driving shaft drives the reciprocating assembly to operate so as to drive the guide block to slide in a reciprocating mode. The rotating rod drives the stirring rod to rotate to stir the electrolyte in the shell, the winding drum drives the connecting rope to wind and unwind, in the winding process, the pressing rod moves downwards to enable the sealing block to extrude the electrolyte into the battery, the transverse plate moves upwards to drive the conveying barrel and the filling pipe to move upwards, and after the transverse plate moves upwards to a proper height, the pressing rod pulls the sealing block to slide upwards to generate negative pressure; and the electrolyte is sucked into the conveying cylinder through the liquid inlet pipe.
Owner:SHANDONG FENGYUAN LITHIUM ENERGY TECH CO LTD

Tooth whitening gel preparation machine and preparation method

The tooth whitening gel preparation machine is characterized in that the tooth whitening gel preparation machine comprises an activation reaction kettle, and the activation reaction kettle comprises an activation reaction kettle electrode driving plate unit, an activation reaction kettle electrode, a liquid inlet and outlet unit, a gas inlet and outlet unit and a liquid activation pool; the activation reaction kettle electrode driving plate unit is positioned at the upper part of the activation reaction kettle electrode; the liquid activation pool is positioned at the lower part of the activation reaction kettle electrode; and the liquid activation pool and the activation reaction kettle electrode form a closed cavity. The hydrogen peroxide liquid prepared by the invention not only has high oxidation activity, but also is mild, non-irritant and non-corrosive.
Owner:JIAXING ZHAOYUAN TECHNOLOGY CO LTD

Post-treatment device for hydrogen internal combustion engine

The invention discloses an after-treatment device for a hydrogen internal combustion engine. The after-treatment device is provided with a side-inlet centrifugal water vapor separation device and a catalytic silencing device which are connected with each other. An air inlet pipe is mounted on the side wall of an air inlet cylinder of the side-inlet centrifugal water-vapor separation device; the rotational flow cylinder is installed in the air inlet cylinder, and a rotational flow cavity is formed between the inner wall of the air inlet cylinder and the outer wall of the rotational flow cylinder. The bottom of the rotational flow plate is connected with the outer wall of the rotational flow cylinder, the top of the rotational flow plate is connected with a boundary line of the air inlet pipe and the air inlet cylinder, the rotational flow plate divides a rotational flow cavity, an obtuse angle formed by the rotational flow plate and the rotational flow cylinder faces the air inlet pipe, an acute angle formed by the rotational flow plate and the rotational flow cylinder is adjacent to a perforated area on the rotational flow cylinder, and meshes are formed in the perforated area; and a stainless steel winding net is mounted at the connecting part of the rotational flow cylinder and the catalytic silencing device. Water vapor in the tail gas of the hydrogen internal combustion engine is condensed into liquid water in advance to be discharged.
Owner:ZHENGZHOU JINGYIDA AUTO PARTS

Environment-friendly biological unblocking liquid and application thereof

The invention relates to the technical field of oil field yield increase and transformation, in particular to environment-friendly biological blockage removal liquid and application thereof, and aims to solve the problem that a current biological blockage removal product can only remove organic blockage. The environment-friendly biological unblocking liquid provided by the invention is prepared from the following components in percentage by mass: 2-5% of biological enzyme, 1-5% of surfactant, 1-5% of pH (Potential of Hydrogen) regulator and the balance of water. 1%-3% of a biological enzyme stabilizer; 3-5% of a chelating agent; 0.5%-1% of a surfactant; 0.5%-1% of a dispersion suspending agent; and the balance of water. The biological blockage removal liquid provided by the invention can effectively remove organic blockage of a stratum caused by wax and asphaltene precipitates, so that crude oil is released from the surfaces of rock particles, the flowing of the crude oil in pores of the stratum is improved by changing the wetting state of rock, and inorganic blockage is removed through permeation dispersion and chelation solubilization effects, so that the mechanical property of the stratum is improved. Mixed scales formed by mutual deposition of inorganic scales and organic matters (wax and asphaltene) are effectively removed, and the requirement for plugging removal of a complex scaling reservoir of an oil well is met.
Owner:CNPC BOHAI DRILLING ENG +1

Preparation method of lithium slurry as well as product and application thereof

The invention belongs to the field of lithium ion batteries, and discloses a preparation method of lithium slurry and a product and application thereof, the preparation method of the slurry comprises the following steps: using a mixed organic solvent formed by mixing an etching agent and a passivating agent, dispersing a material containing lithium metal in the mixed organic solvent, and preparing the lithium slurry. According to the method, an etching agent is used for etching grain boundaries in a material containing lithium metal, a passivating agent is used for passivating grain surfaces, so that slurry containing stable micron-scale and / or nano-scale lithium-containing particles is obtained, and the etching agent and the passivating agent are selected from specific organic solvents. According to the invention, the organic solvent is adopted to carry out etching and passivation reaction on the material containing the lithium metal to obtain the slurry containing the lithium particles, and the particle size is uniform and controllable. The slurry containing the lithium particles can be used for electrode pre-lithiation and preparation of a lithium metal-based electrode and a lithium-based solid-state electrode, so that the energy density of a lithium secondary battery is improved, and the cycle life of the lithium secondary battery is prolonged.
Owner:HUAZHONG UNIV OF SCI & TECH

Protective agent as well as preparation method and application thereof

The invention discloses a protective agent as well as a preparation method and application thereof. The protective agent is prepared from inulin, fructo-oligosaccharide and erythritol. According to the protective agent provided by the invention, the inulin, the fructo-oligosaccharide and the erythritol are compounded to form a mixed protective agent, so that the heat resistance of functional protein in the bovine colostrum processing process can be improved, and the protective agent has a good protective effect on immune globulin (Ig-G) and lactoferrin (LF) in the processing process. Moreover, the three components all have probiotic effects, so that the development of high-value bovine coloctrum functional products is facilitated, and the comprehensive benefits of the bovine coloctrum are improved.
Owner:INNER MONGOLIA MENGNIU DAIRY IND (GROUP) CO LTD