The invention discloses a preparation method of uterine blood mesenchymal stem cells, and relates to the technical field of uterine blood
mesenchymal stem cell preparation, and the preparation method comprises the following steps: filtering and diluting a uterine blood sample, adding the diluted uterine blood sample into a sample
density separation medium, centrifuging, collecting an upper layer and a middle layer, repeatedly centrifuging, and taking
cell precipitate to obtain primary cells of the uterine blood mesenchymal stem cells; resuspending, culturing until the fusion degree of the cells reaches 70-90%, digesting by using a recombinant
trypsin solution, collecting a
cell suspension, centrifuging, resuspending, inoculating and culturing until the fusion degree reaches 70-90%, digesting by using a recombinant
trypsin solution, centrifuging, and resuspending; centrifuging for two times, taking
cell precipitate, and resuspending by using a cell freezing medium to obtain the uterine blood mesenchymal stem cells. According to the method, after the uterine blood sample is filtered, the
centrifugation step is omitted, and the uterine blood sample is directly added to the upper layer of the sample
density separation liquid for centrifugal extraction, so that the normal
living cell proportion of primary cells and the adherence rate after
inoculation are obviously improved, the
production cycle is obviously shortened, and the yield of the primary cells is improved.