276 results about "Cell metabolism" patented technology

The invention provides a virtual-real fusion method for digital twinning operation of a cell drug factory in combination with the Internet of Things, which comprises the following steps: constructing geometric models of a culture medium storage tank and a bioreactor through three-dimensional modeling software, mapping and associating historical process parameters of a culture medium supply system and temperature, pH value and dissolved oxygen concentration monitored in real time with the geometric models, and obtaining a virtual-real fusion model of the culture medium storage tank and the bioreactor; constructing a digital twinborn model of the reactor; analyzing historical production data through a machine learning algorithm, extracting a quantitative relationship among key parameters of a cell metabolism rate, a glucose concentration in a culture medium, a pH value and a reactor temperature, establishing a cell growth prediction model, and predicting a growth trend and a metabolism demand of cells in a target time period; a virtual-real mapping mechanism of a culture medium supply system is integrated with an intelligent manufacturing execution system, so that the culture medium supply process and the whole pharmaceutical production process are subjected to collaborative optimization control, and the working procedures of the culture medium production process are automatically dispatched according to a production plan and cell growth requirements.

The invention discloses an anti-aging composition containing ergothioneine, glutathione and fish collagen, relates to the technical field of anti-aging compositions, and aims to solve the technical problems that a traditional anti-aging composition lacks attention to the aspects of cell energy metabolism, barrier repair and the like, and the overall skin improvement effect is not obvious. The ergothioneine is used as a natural antioxidant, and the content of the ergothioneine is 0.01-5wt%; the ergothioneine participates in a synergistic effect of an intracellular antioxidant enzyme system, so that tyrosinase activity is effectively inhibited, melanogenesis is reduced, and DNA and protein are protected from oxidation damage; in a simulated strong ultraviolet environment, compared with skin cells not using the ergothioneine-containing product, the content of free radicals in the cells is reduced by 30% after the ergothioneine-containing product is used; the ergothioneine can also regulate a cell signal channel, promote cell metabolism and maintain normal growth of cells. The mask has the advantages of accelerating skin metabolism and improving the self-repairing ability of the skin.

The invention relates to a barrier repair composition for activating cell energy and application thereof, the composition comprises the following components: nicotinamide, a wheat germ extract, oat beta-glucan and a golden camellia flower extract, the four components synergistically form a full-link barrier repair system of energy supply (nicotinamide)-autophagy cleaning (wheat germ extract)-structure strengthening (beta-glucan)-oxidation defense (camellia nitidissima extract), the limitation that a traditional skin care product singly supplements lipid or resists oxidation is broken through, lipid dynamic balance is rebuilt from the root of cell metabolism, and the skin care product has the advantages that the skin care effect is good; and certain skin smoothing and anti-aging effects are achieved.

The present disclosure provides a cell segmentation and typing method, device, apparatus and medium based on machine learning. The method includes: acquiring at least one cell metabolism image of a target object; performing single cell image segmentation on the at least one cell metabolism image by using a machine learning segmentation model to obtain a plurality of single cell metabolism images; performing single cell feature extraction on each single cell metabolism image of the plurality of single cell metabolism images to obtain a single cell image feature map corresponding to the single cell metabolism image; combining the single cell image feature map corresponding to each single cell metabolism image of the plurality of single cell metabolism images to obtain an image feature map of the target object; performing typing of the cell by clustering the image feature map of the target object.

The invention belongs to the technical field of microbial fermentation analysis, and discloses a microbial fermentation analysis method and system based on a digital twin technology. Comprising the following steps: acquiring and processing key parameters in a microbial fermentation process; constructing a cell metabolism response index by using the key parameters; analyzing the cell metabolism response index, judging whether the microbial fermentation process is abnormal or not, setting an alarm mechanism, and automatically triggering when the microbial fermentation process is abnormal; a microbial fermentation simulation model based on the digital twinborn technology is combined with the cell metabolism response index, and technological parameters of the microbial fermentation process are optimized; according to the method, comprehensive monitoring, anomaly detection and intelligent optimization of the microbial fermentation process are achieved, the stability and efficiency of the fermentation process are improved, the decision support capacity is enhanced, and technical support is provided for intelligent transformation of the microbial fermentation industry.

The invention relates to the technical field of treatment of chronic diseases of old people, and discloses a metabolic characteristic-based medicine dosage optimization method for chronic diseases of old people. The method comprises the following steps: acquiring drug metabolism parameters (including a plasma concentration peak value, a half-life period curve and the like) and organ function data (including a hepatocyte metabolism rate, a glomerular filtration rate and the like) of a plurality of monitoring nodes, arranging the drug metabolism parameters and the organ function data into a time sequence input vector, and extracting a dynamic feature vector by using a time convolution network and an adaptive filter network; performing feature crossing, pharmacokinetic constraint correction and feature enhancement processing, performing fusion to generate a joint feature vector, inputting the joint feature vector into a dose decision model to obtain an adjustment coefficient, and generating a drug dose interval with a safety threshold in combination with a historical drug use record. According to the method, multi-dimensional data integration and dynamic modeling are realized, the accuracy and safety of chronic disease medication of old people are improved, and the method is suitable for individualized treatment.

The invention relates to the field of tea fermentation, in particular to a preparation method and application of black tea fermentation liquor with an anti-aging effect. The black tea fermentation liquor rich in tea polyphenol, thearubigins, theaflavins and theabrownin is prepared by jointly fermenting black tea with pichia kudriavzevii and bacilli. The black tea fermentation liquor has the functions of regulating cell metabolism and functions, repairing oxidative damage of cells, keeping completeness of mitochondrial functions, promoting synthesis of a high-energy phosphate compound ATP, enhancing cell activity and resisting aging, and can be applied to food, cosmetics and health care products. The fermentation process provided by the invention is green, safe and simple, and has practical application significance.

The invention relates to the technical field of biological medicine, in particular to a lactobacillus-sourced extracellular vesicle and application of the lactobacillus-sourced extracellular vesicle in prevention and treatment of necrotizing enterocolitis, the extracellular vesicle is prepared from lactobacillus, the lactobacillus is Lactobacillus rhamnosus GG, the strain is purchased in the American Center for Type Culture Collection, and the preservation number is ATCC 53103. The preparation method of the extracellular vesicles comprises the following steps: carrying out ultracentrifugation on a lactobacillus culture solution, removing thalli, then collecting supernate, carrying out primary microfiltration, taking micro-filtrate, carrying out differential centrifugation, resuspending precipitates by using PBS, then carrying out secondary microfiltration, collecting the micro-filtrate, carrying out density gradient centrifugation, and collecting a 30-45% interface vesicle layer. The invention discloses a synergistic effect mode between intestinal symbiotic flora and intestinal epithelial cell metabolic molecules for the first time, and the discovery is expected to construct a brand-new mechanism normal form for regulating the host intestinal barrier function by flora.

The invention discloses a data processing method and system for tumor cell detection, and relates to the technical field of data processing.The method comprises the steps that cells are sequenced, and cell metabolism is monitored; performing time delay reconstruction according to the embedding dimension and the delay time, mapping gene expression data to a reconstruction phase space, and calculating a chaos degree of gene expression; establishing a biomarker white list, inputting the chaos degree into an adaptive weight distributor, setting feature retention weights for markers in the white list, constructing a protective auto-encoder, dynamically adjusting the dimension of a bottleneck layer, and performing forced constraint when the markers in the white list are detected; the method comprises the following steps: extracting coupling features through metabolic oscillation data and physical microenvironment data, calculating gradient sensitivity of shear stress to metabolic oscillation, carrying out feature fusion on the coupling features and gene expression features, and constructing a subtype classification boundary for the fused features through feature vector selection in combination with the gradient sensitivity. Accurate dimension reduction of data and deep analysis of biological characteristics are realized.

The invention relates to the technical field of biomedical engineering, in particular to a digital twinning-based biological fermentation reaction kettle process control modeling method, which comprises the following steps: step 1, multi-source heterogeneous data acquisition and treatment: acquiring physical, chemical and biological data of a fermentation reaction kettle, and carrying out cleaning, complementing and time sequence alignment; step 2, mapping knowledge domain driven biological mechanism modeling: constructing a cell metabolism network mapping knowledge domain, associating process parameters with a biological mechanism, and optimizing a feeding strategy; step 3, performing digital twinborn driven multi-physical field simulation: constructing a CFD-molecular dynamics coupling model, and simulating a fermentation tank flow field and a cell metabolism process; 4, quality prediction and control of mechanism-data dual-mode driving are carried out; and 5, deploying and optimizing a cloud edge-end collaborative model. By constructing a'mechanism-data 'dual-drive architecture, the problems of insufficient adaptability, weak multi-modal data fusion capability and the like of a traditional model are solved.

The invention relates to an application of an AMPK inhibitor and ferroptosis inducer combined drug in tumor treatment, in particular to an application of a ferroptosis inducer (RSL3) combined AMPK inhibitor (Compound C) in preparation of a drug for tumor treatment, and belongs to the technical field of biological medicine. In-vivo and in-vitro experiments prove that the combination of a ferroptosis inducer (RSL3) and an AMPK inhibitor (Compound C) drug can significantly inhibit the proliferation of non-small cell lung cancer cells with high expression of MORC2. The drug combination exerts significant synergistic interaction, and has more effective tumor treatment effect than a single drug. Therefore, the combination of the ferroptosis inducer and the cell metabolism inhibitor serves as a new potential clinical treatment strategy, and a new thought is provided for tumor treatment of high-expression MORC2.

The invention discloses a pH-responsive deformable dual-targeting nano protein and a preparation method and application thereof, the method comprises the following steps: activating carboxylated mannose through an EDC / NHS system, reacting with an albumin solution, and dialyzing and purifying to obtain mannose modified albumin; and mixing the mannose modified albumin with an inhibitor IRG1-IN-1, and loading the IRG1-IN-1 through a hydrophobic effect to obtain the mannose albumin nanoparticles, ferritin serves as a carrier, oxaliplatin is loaded through a solvent replacement method, and ferritin nanoparticles are obtained; cHO-PEG2k-CHO is used as a coupling agent, and the mannose albumin nanoparticles and the ferritin nanoparticles are crosslinked through a Schiff base reaction, so that the large-size pH-responsive dual-targeting nano-protein is formed. The nano protein has the characteristic of double-targeting separability, so that specific treatment can be realized, immune cell metabolism is interfered while tumor cells are killed, metabolic immunosuppression is blocked, and anti-tumor immunotherapy is promoted.

The invention discloses a method and a system for constructing a cell aging prediction model based on cell metabolism, and relates to the field of prediction model construction.According to the method and the system, cell aging is predicted from four aspects by selecting content data of four metabolites; finally, the four prediction results are synthesized to judge whether the cells are aged in the future, so that the prediction comprehensiveness is improved, and the prediction accuracy is further improved. A classification model is provided for performing corresponding aging grade prediction on the first aging characterization difference degree data set matrix by constructing a first final multi-layer perceptron classification model; the first final BP prediction model, the second final BP prediction model, the third final BP prediction model and the fourth final BP prediction model are adopted to predict various metabolite data of the to-be-predicted cell sample; and a data basis is provided for subsequently judging the aging condition of the to-be-predicted cell sample at the future moment.

The invention discloses a nerve cell senescence resisting medicine containing p-hydroxybenzaldehyde and application thereof.The p-hydroxybenzaldehyde is adopted as the only active substance, and the p-hydroxybenzaldehyde can reduce accumulation of DNA damage in cells, reduce activity of beta-galactosidase in the cells and slow down reduction of cell metabolic capacity, so that the nerve cell senescence resisting medicine is obtained. Therefore, the senescence of nerve cells is effectively slowed down, the cognitive function is improved, and a potential treatment effect is achieved in neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.

The invention discloses a method for strengthening short-cut denitrification in a high-salt environment by using glycine betaine. By exogenously adding glycine betaine, microbe regulation under short-cut denitrification conditions in a high-salt environment is carried out, microbe extracellular polymer secretion is promoted, the inhibition effect of the high-salt environment on the activity of an electron transfer system is relieved, gene and protein expression is regulated, and a cell metabolism pathway is changed; therefore, rapid accumulation of high-level NO2 <->-N is realized at a high NO3 <->-N degradation rate, the application difficulty of short-cut denitrification in a high-salinity environment is overcome, and the method has important application value in the field of high-salinity wastewater treatment.

The invention relates to an anti-wrinkle and anti-aging composition and application thereof. The anti-wrinkle and anti-aging composition is prepared from hydrolyzed rice protein, a bifidus yeast fermentation product lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, hydroxydecyl ubiquinone, ceramide NP, palmitoyl tripeptide-5, carnosine and acetyl hexapeptide-8. According to the present invention, the components achieve the synergistic effect, such that the fibroblast proliferation can be promoted, the cell metabolism can be improved, the generation of collagen, elastin and the like can be promoted, the excellent oxidation resistance can be provided, and the effects of expression line inhibition, existing wrinkle fading and long-term aging resistance can be combined.

The invention discloses a method for producing MK-7 through whole-cell regulation and control by using different time sequence type promoters, recombinant escherichia coli and application. According to the method, the time sequence type promoters are used for regulating and controlling expression of different key genes related to MK-7 synthesis. According to the invention, the time sequence promoter is used for whole-cell regulation and control to produce the vitamin MK-7 for the first time, dynamic regulation of the MK-7 is realized, and the production efficiency of the MK-7 can be remarkably improved by accurately regulating and controlling expression of key genes in a synthesis route of the MK-7 and optimizing an intracellular metabolic process. The promoter disclosed by the invention can regulate cell metabolism and enhance synthesis of MK-7 in different growth stages or under different environmental conditions by controlling the time and strength of gene expression. The MK-7 is produced by adopting the constructed recombinant escherichia coli obtained based on whole-cell regulation of the sequential promoter, and the yield of 14 g / L can be finally achieved in a 5L fermentation tank.

The invention belongs to the technical field of exosome freeze-dried powder, and discloses exosome freeze-dried powder and a preparation method thereof, the exosome freeze-dried powder contains mesenchymal stem cell exosome, trehalose, sodium hyaluronate, hydroxypropyl-beta-cyclodextrin, glutathione, ceramide and fructo-oligosaccharide. According to the exosome freeze-dried powder, hydroxypropyl-beta-cyclodextrin, glutathione, ceramide and fructo-oligosaccharide are added, the membrane structure integrity of the exosome is effectively protected, the retention rate of active substances in the exosome freeze-dried powder is high, and therefore the effects of resisting inflammation, regulating cell metabolism, promoting tissue repair and the like of the exosome can be fully exerted. In addition, glutathione can directly remove skin free radicals and inhibit oxidative stress injury, and has an anti-oxidation effect; ceramide can also supplement skin lipid barrier, enhance the water locking capacity and enhance the moisturizing effect of sodium hyaluronate; fructo-oligosaccharide can also regulate skin micro-ecological balance, and has anti-inflammatory and bacteriostatic effects.

The invention belongs to the technical field of cosmetics, and discloses a composition for promoting metabolism of hair papilla cells as well as a preparation method and application of the composition. The composition disclosed by the invention is prepared from the following components: a cacumen biotae extract, trehalose, betaine, acetyl tetrapeptide-3 and myristoyl pentapeptide-4. The components in the composition have a good synergistic effect, the mitochondrial membrane potential of the hair papilla cells can be obviously enhanced, the cell activity of the hair papilla cells is promoted, the cell metabolism function of the hair papilla cells is promoted, and therefore the health of hair follicles is effectively maintained.

The invention discloses a thermotherapy device for accelerating cell metabolism, which comprises a host, a physiotherapy head and a touch screen controller, a skin cleaning mechanism is arranged on the physiotherapy head, the touch screen controller is mounted at the upper end of the host, the physiotherapy head is electrically connected with the host through a wire, the physiotherapy head comprises a handle, an electrode plate and a switch, and a physiotherapy sucker is arranged on the side end face of the handle. The skin cleaning mechanism comprises a transmission rotating disc, a motor, a brushing and sweeping disc filter cartridge and a water storage kettle, the transmission rotating disc is installed in the physiotherapy suction cup and rotationally connected with the physiotherapy suction cup, the motor is fixedly installed in the handle and drives the transmission rotating disc, and the brushing and sweeping disc is detachably installed on the transmission rotating disc; and the kettle and the filter cartridge are detachably mounted on the physiotherapy head. The device has the advantages of integrating skin cleaning, electrolyte water circulation flushing and synchronous thermal therapy, and solves the problems that traditional equipment is poor in conductivity, inconvenient to clean and low in treatment comfort level.

Aspects of the present disclosure include methods and compositions related to therapeutic immune cells having enhanced metabolic fitness. In certain aspects, polynucleotides encoding one or more viral, bacterial, and / or fungal genes capable of manipulating cell metabolism and, optionally, one or more antigen-specific receptors, are disclosed. In some aspects, disclosed are methods for enhancing the metabolic fitness of an immune cell comprising introducing into the immune cell a polynucleotide encoding one or more viral, bacterial, and / or fungal genes capable of manipulating cell metabolism. Cells (e.g., NK cells, T cells) expressing polynucleotides encoding one or more viral, bacterial, and / or fungal genes capable of manipulating cell metabolism and, optionally, one or more antigen-specific receptors are described. Also described are therapeutic methods using polynucleotides of the disclosure.

The invention belongs to the technical field of genetic engineering, and particularly relates to a genetically engineered bacterium for producing N-acetylneuraminic acid as well as construction and application of the genetically engineered bacterium. Accumulation of acetic acid is controlled by combining and applying different strategies: rational transformation is performed on an escherichia coli metabolic pathway, and acetic acid synthesis pathway related genes poxB, ackA and pta are knocked out to reduce acetic acid synthesis; the glyoxylic acid cycle is activated by knocking out the coding gene iclR of the glyoxylic acid cycle transcriptional regulation factor, and acetic acid overflow is reduced; the fermentation temperature is adjusted to control overall metabolism of cells to reduce acetic acid accumulation. Through comprehensive application of the strategy, the Neu5Ac production method capable of effectively controlling acetic acid accumulation is obtained, and the method has a good industrial application prospect.

The invention provides a nano medicine for promoting tumor cell copper death and a preparation method thereof, and the preparation method comprises the following steps: selecting a nano raw material carrier, combining copper ion protein, loading a copper ion chelating agent, carrying out ultrasonic wall breaking processing, carrying out surface decoration on the nano carrier, dispersing copper ions, and carrying out dry-method whole particle preparation. According to the nano-drug, the copper ions are carried by the specific nano-carrier, so that the copper ions can be selectively released in tumor cells, the tumor cells are induced to generate copper-dependent death, cell metabolism disorder can be caused by imbalance of a copper steady state, synthesis of iron-sulfur cluster proteins related to mitochondrial respiration is inhibited, and the tumor cell death rate is increased. According to the present invention, with the application of the polypeptide as the protein lipoic acylation modification upstream regulatory factor, the polypeptide can be directly combined with and induce the heteropolymerization, and the drug has good biocompatibility and good targeting property, and the polypeptide can be used as the protein lipoic acylation modification upstream regulatory factor.

The invention relates to the technical field of biology, and particularly discloses a supercooled cell preserving fluid as well as a preserving method and application thereof. The preserving fluid is mainly prepared from RPX basic cell preserving fluid, an energy substrate, a calcium ion channel blocker, a Na < + > / H < + > exchange channel inhibitor, a colloid impermeable cell protective agent, a cell apoptosis inhibitor, an antioxidant, an inflammation inhibitor, a colloid osmotic pressure regulator, a PH regulator and the like according to a certain proportion. According to the preservation method, the cells are preserved under the subcooling condition of-1 DEG C to-7 DEG C, the cell metabolism rate is reduced to the maximum extent, meanwhile, structural and functional damage caused by freezing to the cells is eliminated, and the effect that the survival rate of the recovered cells exceeds 80% after the cells are preserved for more than 18 months under the non-freezing condition is achieved; the application opens up a way for long-term non-freezing preservation of special functional cells of cells and genetic engineering and clinical cell treatment products, provides an effective method, and lays a foundation for starting a new stage of non-freezing long-term preservation of the cells.

Quinones for use in therapeutic recovery, normalization and / or improvement of ATP in mitochondrial deficient cells, such as red blood cells, are disclosed. Particular examples are use in the treatment of erythrocystic diseases, especially in the treatment of erythrocyte metabolic defects, such as erythrocyte glycolysis defects. This treatment increases ATP in non-mitochondrial cells. Thus, the present invention relates to quinones for use in the treatment of diseases or conditions associated with or caused by a glycolytic deficiency in red blood cells.

The present application relates to a system for assessing the degree of senescence, which assesses the degree of senescence of a cell by calculating a score of a cell metabolome state by taking into account metabonomics data of the cell. The system is especially suitable for cosmetic efficacy evaluation, anti-aging drug screening and other scenes, can shorten the research and development period in practical application, and has significant industrial application value.

The invention discloses a liver-protecting beverage containing a composite plant exosome with optimized antioxidant capacity. The liver-protecting beverage comprises a plant exosome solution and a liver-protecting component, the plant exosome solution is a silybum marianum exosome solution and at least two of a Pingyin red rose exosome solution, a radix puerariae exosome solution, a turmeric exosome solution and a dandelion exosome solution; the liver protection component is at least one of silybum marianum micro-capsule powder, a radix puerariae extract, corn peptide, a dandelion extract and S-acetyl-L-glutathione. Hydroxyl and amido on small molecules are combined with lipid structures on the surfaces of multiple plant exosomes, small molecule active ingredients are combined through adsorption or reaction on the surfaces of the lipid structures of the exosomes, the oxidation resistance of the product can be improved, absorption of intestinal cells and probiotics is facilitated, cell metabolism is promoted, and then the fatigue feeling is reduced.

The present invention relates to an RNA hydrogel for tissue regeneration, and more specifically, to an RNA hydrogel for tissue regeneration, which can provide enhanced cell proliferation effect and anti-inflammatory effect, regenerate tissue through wound healing, can be efficiently delivered to cells, does not contain additional chemical components other than nucleic acids, thereby ensuring safety, can deliver a large amount of substances in vivo without toxicity, and can be used for tissue regeneration. The defect that the existing nucleic acid medicine is easy to decompose due to various enzymes in plasma is overcome, and meanwhile, the RNA hydrogel of adenosine is generated in quantity through cell metabolism.

Disclosed in the present invention are a peripheral nerve catheter and a preparation method therefor. The peripheral nerve catheter comprises: an outer sheath, which is a hollow braided tube that has micron-sized pores and is non-wound; at least one inner core tube, which is placed in the outer sheath and is coaxially arranged with the outer sheath, each inner core tube being a hollow fiber tube that has micro / nano-sized pores and is non-wound; and filling gel, which is filled between the outer sheath and the inner core tube to fix the inner core tube. The bionic nerve catheter having a structure composed of the inner core and the outer sheath according to the present invention can achieve effective nerve regeneration, the inner core tube, which axially runs through the outer sheath, has a topological structure that is conducive to the adhesion and directional growth of nerve cells, and also has a micro / nano-sized pore structure that is conducive to the penetration of nutrients for growth and the formation of nerve connections and neurovascular network. In addition, the filling and curing of the filling gel effectively prevents the infection of damaged tissues, and is conducive to the penetration of nutrients and the transfer of cellular metabolic waste.

The present application provides techniques for predicting the amount of at least one biological material produced or consumed by a biological system in a bioreactor. Process conditions and metabolite concentrations of the biological system are measured as a function of time. The metabolic rates of the biological system, including the specific consumption rate of metabolites and the specific production rate of metabolites, are determined. The process conditions and metabolic rates are provided to a hybrid system model configured to predict the production of the biological material. The hybrid system model includes a kinetic growth model configured to predict cell growth in the form of a function of time and a metabolic condition model based on the specific consumption rate or secretion rate of metabolites and selected process conditions. Wherein the metabolic condition model is configured to classify the biological system into a metabolic state. The amount of the biological material is predicted based on the hybrid system model.