176 results about "Cell metabolism" patented technology

The invention relates to the technical field of treatment of chronic diseases of old people, and discloses a metabolic characteristic-based medicine dosage optimization method for chronic diseases of old people. The method comprises the following steps: acquiring drug metabolism parameters (including a plasma concentration peak value, a half-life period curve and the like) and organ function data (including a hepatocyte metabolism rate, a glomerular filtration rate and the like) of a plurality of monitoring nodes, arranging the drug metabolism parameters and the organ function data into a time sequence input vector, and extracting a dynamic feature vector by using a time convolution network and an adaptive filter network; performing feature crossing, pharmacokinetic constraint correction and feature enhancement processing, performing fusion to generate a joint feature vector, inputting the joint feature vector into a dose decision model to obtain an adjustment coefficient, and generating a drug dose interval with a safety threshold in combination with a historical drug use record. According to the method, multi-dimensional data integration and dynamic modeling are realized, the accuracy and safety of chronic disease medication of old people are improved, and the method is suitable for individualized treatment.

The invention relates to the technical field of biological medicine, in particular to a lactobacillus-sourced extracellular vesicle and application of the lactobacillus-sourced extracellular vesicle in prevention and treatment of necrotizing enterocolitis, the extracellular vesicle is prepared from lactobacillus, the lactobacillus is Lactobacillus rhamnosus GG, the strain is purchased in the American Center for Type Culture Collection, and the preservation number is ATCC 53103. The preparation method of the extracellular vesicles comprises the following steps: carrying out ultracentrifugation on a lactobacillus culture solution, removing thalli, then collecting supernate, carrying out primary microfiltration, taking micro-filtrate, carrying out differential centrifugation, resuspending precipitates by using PBS, then carrying out secondary microfiltration, collecting the micro-filtrate, carrying out density gradient centrifugation, and collecting a 30-45% interface vesicle layer. The invention discloses a synergistic effect mode between intestinal symbiotic flora and intestinal epithelial cell metabolic molecules for the first time, and the discovery is expected to construct a brand-new mechanism normal form for regulating the host intestinal barrier function by flora.

The invention relates to the technical field of biomedical engineering, in particular to a digital twinning-based biological fermentation reaction kettle process control modeling method, which comprises the following steps: step 1, multi-source heterogeneous data acquisition and treatment: acquiring physical, chemical and biological data of a fermentation reaction kettle, and carrying out cleaning, complementing and time sequence alignment; step 2, mapping knowledge domain driven biological mechanism modeling: constructing a cell metabolism network mapping knowledge domain, associating process parameters with a biological mechanism, and optimizing a feeding strategy; step 3, performing digital twinborn driven multi-physical field simulation: constructing a CFD-molecular dynamics coupling model, and simulating a fermentation tank flow field and a cell metabolism process; 4, quality prediction and control of mechanism-data dual-mode driving are carried out; and 5, deploying and optimizing a cloud edge-end collaborative model. By constructing a'mechanism-data 'dual-drive architecture, the problems of insufficient adaptability, weak multi-modal data fusion capability and the like of a traditional model are solved.

The invention relates to an anti-wrinkle and anti-aging composition and application thereof. The anti-wrinkle and anti-aging composition is prepared from hydrolyzed rice protein, a bifidus yeast fermentation product lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, hydroxydecyl ubiquinone, ceramide NP, palmitoyl tripeptide-5, carnosine and acetyl hexapeptide-8. According to the present invention, the components achieve the synergistic effect, such that the fibroblast proliferation can be promoted, the cell metabolism can be improved, the generation of collagen, elastin and the like can be promoted, the excellent oxidation resistance can be provided, and the effects of expression line inhibition, existing wrinkle fading and long-term aging resistance can be combined.

The invention belongs to the technical field of exosome freeze-dried powder, and discloses exosome freeze-dried powder and a preparation method thereof, the exosome freeze-dried powder contains mesenchymal stem cell exosome, trehalose, sodium hyaluronate, hydroxypropyl-beta-cyclodextrin, glutathione, ceramide and fructo-oligosaccharide. According to the exosome freeze-dried powder, hydroxypropyl-beta-cyclodextrin, glutathione, ceramide and fructo-oligosaccharide are added, the membrane structure integrity of the exosome is effectively protected, the retention rate of active substances in the exosome freeze-dried powder is high, and therefore the effects of resisting inflammation, regulating cell metabolism, promoting tissue repair and the like of the exosome can be fully exerted. In addition, glutathione can directly remove skin free radicals and inhibit oxidative stress injury, and has an anti-oxidation effect; ceramide can also supplement skin lipid barrier, enhance the water locking capacity and enhance the moisturizing effect of sodium hyaluronate; fructo-oligosaccharide can also regulate skin micro-ecological balance, and has anti-inflammatory and bacteriostatic effects.

The invention belongs to the technical field of cosmetics, and discloses a composition for promoting metabolism of hair papilla cells as well as a preparation method and application of the composition. The composition disclosed by the invention is prepared from the following components: a cacumen biotae extract, trehalose, betaine, acetyl tetrapeptide-3 and myristoyl pentapeptide-4. The components in the composition have a good synergistic effect, the mitochondrial membrane potential of the hair papilla cells can be obviously enhanced, the cell activity of the hair papilla cells is promoted, the cell metabolism function of the hair papilla cells is promoted, and therefore the health of hair follicles is effectively maintained.

The invention discloses a thermotherapy device for accelerating cell metabolism, which comprises a host, a physiotherapy head and a touch screen controller, a skin cleaning mechanism is arranged on the physiotherapy head, the touch screen controller is mounted at the upper end of the host, the physiotherapy head is electrically connected with the host through a wire, the physiotherapy head comprises a handle, an electrode plate and a switch, and a physiotherapy sucker is arranged on the side end face of the handle. The skin cleaning mechanism comprises a transmission rotating disc, a motor, a brushing and sweeping disc filter cartridge and a water storage kettle, the transmission rotating disc is installed in the physiotherapy suction cup and rotationally connected with the physiotherapy suction cup, the motor is fixedly installed in the handle and drives the transmission rotating disc, and the brushing and sweeping disc is detachably installed on the transmission rotating disc; and the kettle and the filter cartridge are detachably mounted on the physiotherapy head. The device has the advantages of integrating skin cleaning, electrolyte water circulation flushing and synchronous thermal therapy, and solves the problems that traditional equipment is poor in conductivity, inconvenient to clean and low in treatment comfort level.

The invention belongs to the technical field of genetic engineering, and particularly relates to a genetically engineered bacterium for producing N-acetylneuraminic acid as well as construction and application of the genetically engineered bacterium. Accumulation of acetic acid is controlled by combining and applying different strategies: rational transformation is performed on an escherichia coli metabolic pathway, and acetic acid synthesis pathway related genes poxB, ackA and pta are knocked out to reduce acetic acid synthesis; the glyoxylic acid cycle is activated by knocking out the coding gene iclR of the glyoxylic acid cycle transcriptional regulation factor, and acetic acid overflow is reduced; the fermentation temperature is adjusted to control overall metabolism of cells to reduce acetic acid accumulation. Through comprehensive application of the strategy, the Neu5Ac production method capable of effectively controlling acetic acid accumulation is obtained, and the method has a good industrial application prospect.

The invention relates to the technical field of biology, and particularly discloses a supercooled cell preserving fluid as well as a preserving method and application thereof. The preserving fluid is mainly prepared from RPX basic cell preserving fluid, an energy substrate, a calcium ion channel blocker, a Na < + > / H < + > exchange channel inhibitor, a colloid impermeable cell protective agent, a cell apoptosis inhibitor, an antioxidant, an inflammation inhibitor, a colloid osmotic pressure regulator, a PH regulator and the like according to a certain proportion. According to the preservation method, the cells are preserved under the subcooling condition of-1 DEG C to-7 DEG C, the cell metabolism rate is reduced to the maximum extent, meanwhile, structural and functional damage caused by freezing to the cells is eliminated, and the effect that the survival rate of the recovered cells exceeds 80% after the cells are preserved for more than 18 months under the non-freezing condition is achieved; the application opens up a way for long-term non-freezing preservation of special functional cells of cells and genetic engineering and clinical cell treatment products, provides an effective method, and lays a foundation for starting a new stage of non-freezing long-term preservation of the cells.

Quinones for use in therapeutic recovery, normalization and / or improvement of ATP in mitochondrial deficient cells, such as red blood cells, are disclosed. Particular examples are use in the treatment of erythrocystic diseases, especially in the treatment of erythrocyte metabolic defects, such as erythrocyte glycolysis defects. This treatment increases ATP in non-mitochondrial cells. Thus, the present invention relates to quinones for use in the treatment of diseases or conditions associated with or caused by a glycolytic deficiency in red blood cells.

The present application relates to a system for assessing the degree of senescence, which assesses the degree of senescence of a cell by calculating a score of a cell metabolome state by taking into account metabonomics data of the cell. The system is especially suitable for cosmetic efficacy evaluation, anti-aging drug screening and other scenes, can shorten the research and development period in practical application, and has significant industrial application value.

The invention discloses a liver-protecting beverage containing a composite plant exosome with optimized antioxidant capacity. The liver-protecting beverage comprises a plant exosome solution and a liver-protecting component, the plant exosome solution is a silybum marianum exosome solution and at least two of a Pingyin red rose exosome solution, a radix puerariae exosome solution, a turmeric exosome solution and a dandelion exosome solution; the liver protection component is at least one of silybum marianum micro-capsule powder, a radix puerariae extract, corn peptide, a dandelion extract and S-acetyl-L-glutathione. Hydroxyl and amido on small molecules are combined with lipid structures on the surfaces of multiple plant exosomes, small molecule active ingredients are combined through adsorption or reaction on the surfaces of the lipid structures of the exosomes, the oxidation resistance of the product can be improved, absorption of intestinal cells and probiotics is facilitated, cell metabolism is promoted, and then the fatigue feeling is reduced.

The present invention relates to an RNA hydrogel for tissue regeneration, and more specifically, to an RNA hydrogel for tissue regeneration, which can provide enhanced cell proliferation effect and anti-inflammatory effect, regenerate tissue through wound healing, can be efficiently delivered to cells, does not contain additional chemical components other than nucleic acids, thereby ensuring safety, can deliver a large amount of substances in vivo without toxicity, and can be used for tissue regeneration. The defect that the existing nucleic acid medicine is easy to decompose due to various enzymes in plasma is overcome, and meanwhile, the RNA hydrogel of adenosine is generated in quantity through cell metabolism.

Disclosed in the present invention are a peripheral nerve catheter and a preparation method therefor. The peripheral nerve catheter comprises: an outer sheath, which is a hollow braided tube that has micron-sized pores and is non-wound; at least one inner core tube, which is placed in the outer sheath and is coaxially arranged with the outer sheath, each inner core tube being a hollow fiber tube that has micro / nano-sized pores and is non-wound; and filling gel, which is filled between the outer sheath and the inner core tube to fix the inner core tube. The bionic nerve catheter having a structure composed of the inner core and the outer sheath according to the present invention can achieve effective nerve regeneration, the inner core tube, which axially runs through the outer sheath, has a topological structure that is conducive to the adhesion and directional growth of nerve cells, and also has a micro / nano-sized pore structure that is conducive to the penetration of nutrients for growth and the formation of nerve connections and neurovascular network. In addition, the filling and curing of the filling gel effectively prevents the infection of damaged tissues, and is conducive to the penetration of nutrients and the transfer of cellular metabolic waste.

Provided are methods for characterizing cellular metabolism in a subject through a repeated exposure approach of administering HP pyruvate to a subject. The methods described may be used for determining the efficacy of cancer therapy or for monitoring disease progression in the subject.

The invention discloses an application of sodium oxamate in preparation of anti-influenza virus products, and provides an application of sodium oxamate as a lactic dehydrogenase A inhibitor in preparation of anti-influenza virus products, the sodium oxamate has broad-spectrum antiviral activity, and can play a unique action mechanism by regulating host cell metabolism (such as protein lactylation). And a new direction is provided for overcoming virus resistance.

The invention provides a composition, cell culture and application of the composition in differentiation of human pluripotent stem cells to myocardial cells and / or construction of human-derived heart organoid, and belongs to the technical field of cell engineering. The invention provides a composition. The composition comprises a fatty acid receptor antagonist, a fatty acid synthetase inhibitor and a fatty acid oxidation activator. The composition provided by the invention can regulate cell metabolism, breaks through the contradiction that the proliferation capacity and the cell maturity cannot be obtained at the same time in the traditional technology, can maintain the myocardial cells in a proliferation stage, and obtains the myocardial cells with relatively mature structures and functions, and the contractility of 3D heart organs constructed by the myocardial cells is greatly improved. The invention provides a high-quality cell source for myocardial regeneration treatment, high-throughput drug cardiotoxicity screening and heart disease mechanism research, and has remarkable clinical application value and industrialization prospect.

Systems and methods are provided for predicting characteristics of one or more cells of a tissue sample based on intracellular dynamic activity information. An optical imaging device is used in conjunction with a high-speed camera to capture a set of images. The set of images are then processed to determine frequency data associated with one or more cells in the images. One or more machine learning (ML) / deep learning (DL) algorithm is applied to the set of images to predict characteristics of one or more cells in the tissue sample. In some embodiments, a semantic segmentation algorithm is used to identify intracellular structures within each cell and the ML / DL algorithms are trained to utilize a semantic map of each cell to predict the characteristics.

The invention relates to the technical field of biological medicines, in particular to a maintenance solution for culturing influenza viruses as well as a preparation method and application of the maintenance solution. The maintenance liquid comprises a basic culture medium and effective components, the effective components comprise amino acids, vitamins, salts and a virus nutrition enhancer, and the virus nutrition enhancer is a microorganism induced culture extract. The extract is prepared by inoculating fermentation microbial bacteria, performing induced culture through a plant induction factor extract containing American ginseng, polygonum cuspidatum and golden cypress, and then performing autolysis and enzymolysis in the presence of compound metabolic regulatory factors, namely trichosalix A and N-acetylcysteine. The maintenance fluid can significantly enhance host cell metabolism, inhibit cell antiviral response and delay cell apoptosis after infection, so that the culture yield and process stability of influenza viruses are significantly improved under the condition of no dependence on serum.

Pharmaceutical compositions and methods of using these compositions are disclosed that show efficacy in the treatment / amelioration of various human diseases, including cancer. The pharmaceutical composition comprises compounds that modulate the function of fatty-acid binding proteins that are used in combination with immunotherapy treatment that demonstrates synergistic efficacy to target and eliminate disease-associated antigens and / or cells.

The utility model discloses and provides a multifunctional meridian comb which is economical, practical and comprehensive in function, combines modern science and technology and traditional Chinese medicine theories, achieves double effects of meridian massage and beauty treatment, and is internally provided with a heating part and a discharging part. According to the invention, acupuncture points on the body can be accurately massaged, the skin of the body can be beautified and maintained, and meanwhile, blood circulation and cell metabolism can be promoted, so that the effects of relieving fatigue, maintaining beauty and keeping young are achieved. The electric heating comb comprises a comb handle assembly, a comb body assembly and a comb cover assembly, the bottom end of the comb body assembly is matched with the interior of the comb handle assembly in a limiting mode, the comb cover assembly is matched with the comb handle assembly and the comb body assembly in a limiting mode, a circuit board is arranged in the comb handle assembly, a heating part is arranged on the circuit board, and the heating part is arranged on the circuit board. And the heating part is connected and matched with the comb body assembly. The device is applied to the field of body massage and skin beautifying devices.

Techniques for predicting an amount of at least one biomaterial produced or consumed by a biological system in a bioreactor are provided. Process conditions and metabolite concentrations are measured for the biological system as a function of time. Metabolic rates for the biological system, including specific consumption rates of metabolites and specific production rates of metabolites are determined. The process conditions and the metabolic rates are provided to a hybrid system model configured to predict production of the biomaterial. The hybrid system model includes a kinetic growth model configured to estimate cell growth as a function of time and a metabolic condition model based on metabolite specific consumption or secretion rates and select process conditions, wherein the metabolic condition model is configured to classify the biological system into a metabolic state. An amount of the biomaterial based on the hybrid system model is predicted.

T lymphocytes play a key role in the immune response and their functions are intimately linked to metabolic programs. During immune responses, T cells undergo a metabolic reprogramming notably characterized by an increased aerobic glycolysis. Using a quantitative phosphoproteomic approach, the inventors have identified a new transcription factor called Foxk1 as being highly phosphorylated in T cells upon T Cell Receptor (TCR) engagement. The results also indicate that Foxk1 phosphorylation and nuclear translocation is dependent of the AKT-mTOR kinase activities. Using T-cell specific Foxk1 deficient mice (Foxk1- / -), we demonstrated that Foxk1 is required for full T cell activation. Foxk1-deficient T cells exhibited reduced proliferation and cytokine secretion following TCR stimulation. Furthermore, T cells from Foxk1- / - mice were less prone to acquire an effector like phenotype than wild-type cells when challenged in vivo. Conversely, Foxk1 overexpression in T cells enhanced their effector functions in a TCR-dependent manner. In CD8+ T cells, this effect also results into enhanced cancer cell killing capacity in vitro, and improved tumor rejection in vivo. Altogether, these results indicated that Foxk1 is a major regulator of T cell metabolism and thus, of T cell effector functions. Thus, the present invention relates to host immune cells engineered to overexpress a Foxk1 polypeptide and their use of the treatment of cancer.

The invention discloses a preparation technology of astragalus polysaccharide camouflage monosaccharide, and relates to the technical field of biological product production, the preparation technology comprises the following steps: S1, extraction, S2, purification: carrying out purification treatment on astragalus polysaccharide extracted in the step 1, S3, degradation camouflage: dissolving the astragalus polysaccharide purified in the step 2 in a solvent, carrying out degradation treatment on the astragalus polysaccharide by adopting ultrasonic waves, and carrying out purification treatment on the astragalus polysaccharide to obtain the astragalus polysaccharide camouflage monosaccharide. A specific glucan structural unit in a polysaccharide chain is degraded and disguised into a monosaccharide or oligosaccharide structure; and S4, separating to obtain a product. Astragalus polysaccharide is directionally camouflaged as a monosaccharide / oligosaccharide structure, the recognition and uptake efficiency of astragalus polysaccharide by cancer cells is remarkably enhanced, so that the targeting of inducing apoptosis of cancer cells is improved, the structure can effectively activate and train immune cells such as dendritic cells and strengthen anti-tumor immune response, and meanwhile, the product can interfere with a metabolic pathway of cancer cells and reverse the drug resistance of cancer cells, so that the anti-tumor effect is improved. Nutrition supply is blocked to inhibit tumor growth, the production process is green and controllable, and good clinical transformation and industrialization prospects are achieved.

The invention discloses a dry macular degeneration therapeutic apparatus based on a multispectral metabolic repair system, and belongs to the related technical field of metabolic repair therapeutic equipment. The dry macular degeneration therapeutic apparatus based on the multispectral metabolic repair system comprises a dry macular degeneration therapeutic apparatus host; a display screen and a plurality of control buttons are arranged on the front face of a shell of the dry macular degeneration therapeutic instrument host, the control buttons are evenly arranged in a single row and all located below the display screen, a clamping base is arranged on the side face of the dry macular degeneration therapeutic instrument host, and a remote controller is clamped through the clamping base. The bottom of the dry macular degeneration therapeutic instrument host is provided with two embedded grooves, and each embedded groove is internally provided with a plurality of trundles. The device can be combined with a multispectral metabolism repairing technology, improves cell metabolism through photobiological regulation of a specific wavelength combination, avoids the problems of single wavelength, inaccurate energy parameters and the like, and realizes accurate intervention on a retina metabolism function.

The invention belongs to the technical field of cosmetics, and particularly relates to an anti-aging cell metabolism accelerating cosmetic and a preparation method thereof, and the technical scheme is that the anti-aging cell metabolism accelerating cosmetic comprises the following components: 0.0001%-0.005% of a cell growth factor compound, 0.0001%-0.003% of a small molecule peptide compound, 0.1%-5% of a plant stem cell extract, 1%-10% of grease, and the balance of water. 1%-10% of a surfactant, 1%-15% of a humectant, 0.1%-1% of an antioxidant, 0.1%-3% of a pH regulator, 0.05%-0.2% of a preservative and the balance of deionized water; epidermal and dermal layer cell proliferation is promoted through the cell growth factor, cell metabolism is regulated and controlled and collagen synthesis is promoted through the small molecule peptide, the plant stem cell extract is rich in polyphenol active ingredients and can activate the activity of skin endogenous stem cells, and the three components have a synergistic effect through different action pathways; the metabolism rate of skin cells and the anti-aging effect are obviously improved.

A method for evaluating the effect of a drug on a tumor and tumor-related cells. The tumor and the tumor-related cells are cultured in a culture medium containing a to-be-tested drug and a metabolic marker containing deuterium, the tumor and the tumor-related cells are imaged by means of coherent Raman scattering microscopy, and a metabolic product signal of the metabolic marker in an image is quantified, so as to evaluate the effect of the to-be-tested drug on the tumor and the tumor-related cells. According to the provided method, by means of quantifying unique chemical bonds that are newly generated after cellular metabolism, different cell samples can be subjected to drug effect detection based on the differences in metabolic inhibition by means of the same detection scheme. The method is simple and rapid, requiring a short culture time, which avoids the influence of prolonged culture on various properties of cells. The method has a better universality while reducing the detection cost.

The application relates to the technical field of medicines, in particular to a lymphocyte preservation solution, a preparation method and application in sample pretreatment, and the lymphocyte preservation solution is prepared from the following components in parts by mass: Tris-HCl buffer 90-100 parts, trehalose 10-15 parts, trichostatin A 0.01-0.03 parts, nuclease inhibitor 0.3-0.5 parts and the like raw materials. Through the synergistic design of a composite buffer system and functionalized nanomaterials, combined with multi-stage active protection technology, cell metabolism regulation and precise isolation of external interference factors are realized. The application improves the processing efficiency of complex samples, prolongs the preservation time of key biomarkers, and enhances the adaptability in extreme environments. The integration of the innovative preparation process and the intelligent quality control module provides reliable support for immune diagnosis and treatment, rare cell research and remote medical treatment, and has wide clinical application and scientific research transformation prospects.

This invention relates to the field of optical components and provides a pair of far-infrared nanomaterial functional glasses for biophoton-targeted cell activation. The glasses include a frame, lenses, nose pads, and temples. The nose pads are connected to the frame and threaded into the inner wall of a threaded groove. The temples are rotatably connected to the screw, and a gear is fixed to the upper end of the screw. A slider is slidably connected to the inner wall of a transmission cavity, and the slider is connected to the inner wall of the transmission cavity via a second spring. A pusher is connected to the slider, and a first permanent magnet is slidably connected to the pusher. A rack is fixed to the first permanent magnet. A third and fourth permanent magnet are fixed to the inner wall of the transmission cavity. The two frames are fixed together via a nose bridge. The frame, nose pads, and temples are made of far-infrared nanomaterials. The lenses consist of a lens substrate and a tourmaline powder film connected sequentially. The lens substrate is made of polycarbonate. This invention can target and activate cells, promote cell metabolism, and repair eye fatigue.

The invention relates to the field of cosmetics, in particular to a cosmetic composition containing sturgeon polypeptide and a preparation method and application of the cosmetic composition. According to the cosmetic composition containing the sturgeon polypeptide, the cosmetic composition contains the sturgeon polypeptide, and the sturgeon polypeptide is derived from sturgeon eggs. The sturgeon polypeptide from sturgeon roes is selected as an active ingredient of the cosmetic, and the polypeptide is small in molecular weight, good in biocompatibility, capable of participating in skin cell metabolism regulation and beneficial to promoting synthesis of COL-I and ELN. Compared with an existing cosmetic mainly containing common protein or non-specific polypeptide, the composition has more obvious efficacy advantages in the aspect of improving skin fine wrinkles and wrinkles.