113 results about "Cell metabolism" patented technology

The invention relates to an anti-wrinkle and anti-aging composition and application thereof. The anti-wrinkle and anti-aging composition is prepared from hydrolyzed rice protein, a bifidus yeast fermentation product lysate, dipeptide diaminobutyryl benzyl amide diacetate, adenosine, hydroxydecyl ubiquinone, ceramide NP, palmitoyl tripeptide-5, carnosine and acetyl hexapeptide-8. According to the present invention, the components achieve the synergistic effect, such that the fibroblast proliferation can be promoted, the cell metabolism can be improved, the generation of collagen, elastin and the like can be promoted, the excellent oxidation resistance can be provided, and the effects of expression line inhibition, existing wrinkle fading and long-term aging resistance can be combined.

The invention belongs to the technical field of exosome freeze-dried powder, and discloses exosome freeze-dried powder and a preparation method thereof, the exosome freeze-dried powder contains mesenchymal stem cell exosome, trehalose, sodium hyaluronate, hydroxypropyl-beta-cyclodextrin, glutathione, ceramide and fructo-oligosaccharide. According to the exosome freeze-dried powder, hydroxypropyl-beta-cyclodextrin, glutathione, ceramide and fructo-oligosaccharide are added, the membrane structure integrity of the exosome is effectively protected, the retention rate of active substances in the exosome freeze-dried powder is high, and therefore the effects of resisting inflammation, regulating cell metabolism, promoting tissue repair and the like of the exosome can be fully exerted. In addition, glutathione can directly remove skin free radicals and inhibit oxidative stress injury, and has an anti-oxidation effect; ceramide can also supplement skin lipid barrier, enhance the water locking capacity and enhance the moisturizing effect of sodium hyaluronate; fructo-oligosaccharide can also regulate skin micro-ecological balance, and has anti-inflammatory and bacteriostatic effects.

The invention relates to the technical field of biology, and particularly discloses a supercooled cell preserving fluid as well as a preserving method and application thereof. The preserving fluid is mainly prepared from RPX basic cell preserving fluid, an energy substrate, a calcium ion channel blocker, a Na < + > / H < + > exchange channel inhibitor, a colloid impermeable cell protective agent, a cell apoptosis inhibitor, an antioxidant, an inflammation inhibitor, a colloid osmotic pressure regulator, a PH regulator and the like according to a certain proportion. According to the preservation method, the cells are preserved under the subcooling condition of-1 DEG C to-7 DEG C, the cell metabolism rate is reduced to the maximum extent, meanwhile, structural and functional damage caused by freezing to the cells is eliminated, and the effect that the survival rate of the recovered cells exceeds 80% after the cells are preserved for more than 18 months under the non-freezing condition is achieved; the application opens up a way for long-term non-freezing preservation of special functional cells of cells and genetic engineering and clinical cell treatment products, provides an effective method, and lays a foundation for starting a new stage of non-freezing long-term preservation of the cells.

Quinones for use in therapeutic recovery, normalization and / or improvement of ATP in mitochondrial deficient cells, such as red blood cells, are disclosed. Particular examples are use in the treatment of erythrocystic diseases, especially in the treatment of erythrocyte metabolic defects, such as erythrocyte glycolysis defects. This treatment increases ATP in non-mitochondrial cells. Thus, the present invention relates to quinones for use in the treatment of diseases or conditions associated with or caused by a glycolytic deficiency in red blood cells.

The present application relates to a system for assessing the degree of senescence, which assesses the degree of senescence of a cell by calculating a score of a cell metabolome state by taking into account metabonomics data of the cell. The system is especially suitable for cosmetic efficacy evaluation, anti-aging drug screening and other scenes, can shorten the research and development period in practical application, and has significant industrial application value.

The invention discloses a liver-protecting beverage containing a composite plant exosome with optimized antioxidant capacity. The liver-protecting beverage comprises a plant exosome solution and a liver-protecting component, the plant exosome solution is a silybum marianum exosome solution and at least two of a Pingyin red rose exosome solution, a radix puerariae exosome solution, a turmeric exosome solution and a dandelion exosome solution; the liver protection component is at least one of silybum marianum micro-capsule powder, a radix puerariae extract, corn peptide, a dandelion extract and S-acetyl-L-glutathione. Hydroxyl and amido on small molecules are combined with lipid structures on the surfaces of multiple plant exosomes, small molecule active ingredients are combined through adsorption or reaction on the surfaces of the lipid structures of the exosomes, the oxidation resistance of the product can be improved, absorption of intestinal cells and probiotics is facilitated, cell metabolism is promoted, and then the fatigue feeling is reduced.

The present invention relates to an RNA hydrogel for tissue regeneration, and more specifically, to an RNA hydrogel for tissue regeneration, which can provide enhanced cell proliferation effect and anti-inflammatory effect, regenerate tissue through wound healing, can be efficiently delivered to cells, does not contain additional chemical components other than nucleic acids, thereby ensuring safety, can deliver a large amount of substances in vivo without toxicity, and can be used for tissue regeneration. The defect that the existing nucleic acid medicine is easy to decompose due to various enzymes in plasma is overcome, and meanwhile, the RNA hydrogel of adenosine is generated in quantity through cell metabolism.

Disclosed in the present invention are a peripheral nerve catheter and a preparation method therefor. The peripheral nerve catheter comprises: an outer sheath, which is a hollow braided tube that has micron-sized pores and is non-wound; at least one inner core tube, which is placed in the outer sheath and is coaxially arranged with the outer sheath, each inner core tube being a hollow fiber tube that has micro / nano-sized pores and is non-wound; and filling gel, which is filled between the outer sheath and the inner core tube to fix the inner core tube. The bionic nerve catheter having a structure composed of the inner core and the outer sheath according to the present invention can achieve effective nerve regeneration, the inner core tube, which axially runs through the outer sheath, has a topological structure that is conducive to the adhesion and directional growth of nerve cells, and also has a micro / nano-sized pore structure that is conducive to the penetration of nutrients for growth and the formation of nerve connections and neurovascular network. In addition, the filling and curing of the filling gel effectively prevents the infection of damaged tissues, and is conducive to the penetration of nutrients and the transfer of cellular metabolic waste.

The invention discloses an application of sodium oxamate in preparation of anti-influenza virus products, and provides an application of sodium oxamate as a lactic dehydrogenase A inhibitor in preparation of anti-influenza virus products, the sodium oxamate has broad-spectrum antiviral activity, and can play a unique action mechanism by regulating host cell metabolism (such as protein lactylation). And a new direction is provided for overcoming virus resistance.

The invention provides a composition, cell culture and application of the composition in differentiation of human pluripotent stem cells to myocardial cells and / or construction of human-derived heart organoid, and belongs to the technical field of cell engineering. The invention provides a composition. The composition comprises a fatty acid receptor antagonist, a fatty acid synthetase inhibitor and a fatty acid oxidation activator. The composition provided by the invention can regulate cell metabolism, breaks through the contradiction that the proliferation capacity and the cell maturity cannot be obtained at the same time in the traditional technology, can maintain the myocardial cells in a proliferation stage, and obtains the myocardial cells with relatively mature structures and functions, and the contractility of 3D heart organs constructed by the myocardial cells is greatly improved. The invention provides a high-quality cell source for myocardial regeneration treatment, high-throughput drug cardiotoxicity screening and heart disease mechanism research, and has remarkable clinical application value and industrialization prospect.

The invention relates to the technical field of biological medicines, in particular to a maintenance solution for culturing influenza viruses as well as a preparation method and application of the maintenance solution. The maintenance liquid comprises a basic culture medium and effective components, the effective components comprise amino acids, vitamins, salts and a virus nutrition enhancer, and the virus nutrition enhancer is a microorganism induced culture extract. The extract is prepared by inoculating fermentation microbial bacteria, performing induced culture through a plant induction factor extract containing American ginseng, polygonum cuspidatum and golden cypress, and then performing autolysis and enzymolysis in the presence of compound metabolic regulatory factors, namely trichosalix A and N-acetylcysteine. The maintenance fluid can significantly enhance host cell metabolism, inhibit cell antiviral response and delay cell apoptosis after infection, so that the culture yield and process stability of influenza viruses are significantly improved under the condition of no dependence on serum.

Pharmaceutical compositions and methods of using these compositions are disclosed that show efficacy in the treatment / amelioration of various human diseases, including cancer. The pharmaceutical composition comprises compounds that modulate the function of fatty-acid binding proteins that are used in combination with immunotherapy treatment that demonstrates synergistic efficacy to target and eliminate disease-associated antigens and / or cells.

The utility model discloses and provides a multifunctional meridian comb which is economical, practical and comprehensive in function, combines modern science and technology and traditional Chinese medicine theories, achieves double effects of meridian massage and beauty treatment, and is internally provided with a heating part and a discharging part. According to the invention, acupuncture points on the body can be accurately massaged, the skin of the body can be beautified and maintained, and meanwhile, blood circulation and cell metabolism can be promoted, so that the effects of relieving fatigue, maintaining beauty and keeping young are achieved. The electric heating comb comprises a comb handle assembly, a comb body assembly and a comb cover assembly, the bottom end of the comb body assembly is matched with the interior of the comb handle assembly in a limiting mode, the comb cover assembly is matched with the comb handle assembly and the comb body assembly in a limiting mode, a circuit board is arranged in the comb handle assembly, a heating part is arranged on the circuit board, and the heating part is arranged on the circuit board. And the heating part is connected and matched with the comb body assembly. The device is applied to the field of body massage and skin beautifying devices.

Techniques for predicting an amount of at least one biomaterial produced or consumed by a biological system in a bioreactor are provided. Process conditions and metabolite concentrations are measured for the biological system as a function of time. Metabolic rates for the biological system, including specific consumption rates of metabolites and specific production rates of metabolites are determined. The process conditions and the metabolic rates are provided to a hybrid system model configured to predict production of the biomaterial. The hybrid system model includes a kinetic growth model configured to estimate cell growth as a function of time and a metabolic condition model based on metabolite specific consumption or secretion rates and select process conditions, wherein the metabolic condition model is configured to classify the biological system into a metabolic state. An amount of the biomaterial based on the hybrid system model is predicted.

T lymphocytes play a key role in the immune response and their functions are intimately linked to metabolic programs. During immune responses, T cells undergo a metabolic reprogramming notably characterized by an increased aerobic glycolysis. Using a quantitative phosphoproteomic approach, the inventors have identified a new transcription factor called Foxk1 as being highly phosphorylated in T cells upon T Cell Receptor (TCR) engagement. The results also indicate that Foxk1 phosphorylation and nuclear translocation is dependent of the AKT-mTOR kinase activities. Using T-cell specific Foxk1 deficient mice (Foxk1- / -), we demonstrated that Foxk1 is required for full T cell activation. Foxk1-deficient T cells exhibited reduced proliferation and cytokine secretion following TCR stimulation. Furthermore, T cells from Foxk1- / - mice were less prone to acquire an effector like phenotype than wild-type cells when challenged in vivo. Conversely, Foxk1 overexpression in T cells enhanced their effector functions in a TCR-dependent manner. In CD8+ T cells, this effect also results into enhanced cancer cell killing capacity in vitro, and improved tumor rejection in vivo. Altogether, these results indicated that Foxk1 is a major regulator of T cell metabolism and thus, of T cell effector functions. Thus, the present invention relates to host immune cells engineered to overexpress a Foxk1 polypeptide and their use of the treatment of cancer.

The invention discloses a preparation technology of astragalus polysaccharide camouflage monosaccharide, and relates to the technical field of biological product production, the preparation technology comprises the following steps: S1, extraction, S2, purification: carrying out purification treatment on astragalus polysaccharide extracted in the step 1, S3, degradation camouflage: dissolving the astragalus polysaccharide purified in the step 2 in a solvent, carrying out degradation treatment on the astragalus polysaccharide by adopting ultrasonic waves, and carrying out purification treatment on the astragalus polysaccharide to obtain the astragalus polysaccharide camouflage monosaccharide. A specific glucan structural unit in a polysaccharide chain is degraded and disguised into a monosaccharide or oligosaccharide structure; and S4, separating to obtain a product. Astragalus polysaccharide is directionally camouflaged as a monosaccharide / oligosaccharide structure, the recognition and uptake efficiency of astragalus polysaccharide by cancer cells is remarkably enhanced, so that the targeting of inducing apoptosis of cancer cells is improved, the structure can effectively activate and train immune cells such as dendritic cells and strengthen anti-tumor immune response, and meanwhile, the product can interfere with a metabolic pathway of cancer cells and reverse the drug resistance of cancer cells, so that the anti-tumor effect is improved. Nutrition supply is blocked to inhibit tumor growth, the production process is green and controllable, and good clinical transformation and industrialization prospects are achieved.

The invention belongs to the technical field of cosmetics, and particularly relates to an anti-aging cell metabolism accelerating cosmetic and a preparation method thereof, and the technical scheme is that the anti-aging cell metabolism accelerating cosmetic comprises the following components: 0.0001%-0.005% of a cell growth factor compound, 0.0001%-0.003% of a small molecule peptide compound, 0.1%-5% of a plant stem cell extract, 1%-10% of grease, and the balance of water. 1%-10% of a surfactant, 1%-15% of a humectant, 0.1%-1% of an antioxidant, 0.1%-3% of a pH regulator, 0.05%-0.2% of a preservative and the balance of deionized water; epidermal and dermal layer cell proliferation is promoted through the cell growth factor, cell metabolism is regulated and controlled and collagen synthesis is promoted through the small molecule peptide, the plant stem cell extract is rich in polyphenol active ingredients and can activate the activity of skin endogenous stem cells, and the three components have a synergistic effect through different action pathways; the metabolism rate of skin cells and the anti-aging effect are obviously improved.

A method for evaluating the effect of a drug on a tumor and tumor-related cells. The tumor and the tumor-related cells are cultured in a culture medium containing a to-be-tested drug and a metabolic marker containing deuterium, the tumor and the tumor-related cells are imaged by means of coherent Raman scattering microscopy, and a metabolic product signal of the metabolic marker in an image is quantified, so as to evaluate the effect of the to-be-tested drug on the tumor and the tumor-related cells. According to the provided method, by means of quantifying unique chemical bonds that are newly generated after cellular metabolism, different cell samples can be subjected to drug effect detection based on the differences in metabolic inhibition by means of the same detection scheme. The method is simple and rapid, requiring a short culture time, which avoids the influence of prolonged culture on various properties of cells. The method has a better universality while reducing the detection cost.

The application relates to the technical field of medicines, in particular to a lymphocyte preservation solution, a preparation method and application in sample pretreatment, and the lymphocyte preservation solution is prepared from the following components in parts by mass: Tris-HCl buffer 90-100 parts, trehalose 10-15 parts, trichostatin A 0.01-0.03 parts, nuclease inhibitor 0.3-0.5 parts and the like raw materials. Through the synergistic design of a composite buffer system and functionalized nanomaterials, combined with multi-stage active protection technology, cell metabolism regulation and precise isolation of external interference factors are realized. The application improves the processing efficiency of complex samples, prolongs the preservation time of key biomarkers, and enhances the adaptability in extreme environments. The integration of the innovative preparation process and the intelligent quality control module provides reliable support for immune diagnosis and treatment, rare cell research and remote medical treatment, and has wide clinical application and scientific research transformation prospects.

The invention relates to the field of cosmetics, in particular to a cosmetic composition containing sturgeon polypeptide and a preparation method and application of the cosmetic composition. According to the cosmetic composition containing the sturgeon polypeptide, the cosmetic composition contains the sturgeon polypeptide, and the sturgeon polypeptide is derived from sturgeon eggs. The sturgeon polypeptide from sturgeon roes is selected as an active ingredient of the cosmetic, and the polypeptide is small in molecular weight, good in biocompatibility, capable of participating in skin cell metabolism regulation and beneficial to promoting synthesis of COL-I and ELN. Compared with an existing cosmetic mainly containing common protein or non-specific polypeptide, the composition has more obvious efficacy advantages in the aspect of improving skin fine wrinkles and wrinkles.

The application discloses a method for predicting different metabolic states of cells in cancer and metabolic characteristic subtypes of the cells based on a metabolite-protein interaction network, and the method comprises the following steps: (1) identifying the classification and metabolic state of the cells: preprocessing data, filtering low-quality cells; performing twice principal component analysis according to key genes; performing twice dimension reduction clustering on the cells respectively; (2) predicting metabolic characteristic subtypes of a queue according to the proportion of different metabolic states of cells in the sample. The method can identify the metabolic state of the cells and metabolic subtypes, and provides a new perspective for cancer research.

The invention discloses a mattress material based on modified fibers and a preparation method of the mattress material. The mattress material comprises nylon 66 fibers, low-melting-point fibers and the modified fibers. The low-melting-point fiber is ES bi-component parallel composite fiber composed of polypropylene and polyethylene, and the modified fiber is prepared by mixing a modified polymer and a modified filler to prepare a spinning solution and then carrying out electrostatic spinning and ultraviolet irradiation. Molecular chains of the modified polymer contain rich furan rings and thiophene rings, synthesis of bacterial cell walls can be destroyed, the furan is grafted with oximido, and a formed furan oxime ester unit can generate highly unstable and reactive free radicals, so that metal ions in cells are unbalanced to cause change of cell metabolism homeostasis. The lysozyme fixed on the modified filler can be hydrolyzed to form peptidoglycan of bacterial cell walls, so that the cell walls are broken, and the material has good antibacterial performance.

The invention discloses application of R-1, 3-butanediol in preparation of anti-aging cosmetics or medicines, and relates to the technical field of cosmetics. According to the embodiment of the invention, the R-1, 3-butanediol is applied to the skin, and the R-1, 3-butanediol is explored and proved to be capable of exceeding the traditional physical and chemical functions of R-1, 3-butanediol, being utilized by cells, participating in the energy metabolism process of the skin cells, regulating the energy metabolism of mitochondria and regulating the expression of AMPK protein in the skin cells; the skin function is improved from the fundamental level of cell energy supply, and the skin aging problem caused by cell metabolism decline is resisted. The positioning and action mechanism is different from all known traditional applications, and represents a brand-new application mode of the component in cosmetics based on cytobiology. The functional skin care product is expected to be promoted to be upgraded to the direction of cellular metabolism regulation, and is popularized and applied.

The present application relates to the field of medical detection, in particular to a probe combination for full-spectrum flow cytometry detection of cell metabolism and application thereof. Traditional flow cytometry is limited by fluorescence channel overlap and cannot simultaneously detect multiple metabolic states in the same sample, affecting detection efficiency and data accuracy. The present application optimizes the probe combination and uses full-spectrum flow cytometry to achieve simultaneous detection of two or more metabolic probes, simultaneously detecting multiple metabolic indicators such as intracellular fatty acid uptake, glucose uptake, active oxygen level, and mitochondrial function, and ensuring the compatibility of fluorescence signals between probes. The method of the present application can reduce sample consumption and improve detection efficiency, and is suitable for metabolic analysis of trace clinical samples and rare cell populations, and is widely used in the fields of tumor research, metabolic disease monitoring, drug screening, etc., and provides an efficient and accurate technical means for single-cell level metabolic research.

This invention belongs to the field of lithium adsorbent technology, and discloses an aluminum-based lithium adsorbent, its preparation method, and its application. The method involves mixing water-soluble lithium salt, aluminum salt, graphite analog, and an antibacterial agent to obtain a mixed solution. After pH adjustment, reaction, and filtration, an active powder is obtained. This powder is then mixed with a binder dissolved in a solvent, granulated, dried, and sieved to obtain the aluminum-based lithium adsorbent. In this aluminum-based lithium adsorbent, the sharp lamellar structure of the graphite analog surface can disrupt cell integrity, and the oxygen-containing functional groups release reactive oxygen species and free radicals to inhibit microbial growth and increase the mechanical strength of the adsorbent. The doped antibacterial agent interferes with cell metabolism, and the two work synergistically to enhance the antibacterial effect. This material inhibits bacteria through physicochemical means, is less likely to induce drug resistance, extends service life, reduces the risk of biocontamination, and improves adsorption capacity and efficiency.

The invention discloses a copper-manganese alloy drug loading system and a preparation method and application thereof.The preparation method of the copper-manganese alloy drug loading system at least comprises the following steps that S1, gas-atomized copper-manganese powder serves as a raw material, the 3D printing technology is used for printing the gas-atomized copper-manganese powder, and a copper-manganese alloy precursor is obtained; s2, the copper-manganese alloy precursor is placed in a dealloying solution for dealloying, and the copper-manganese alloy of the porous nanostructure is obtained; and S3, loading an anti-tumor drug on the copper-manganese alloy with the porous nanostructure to obtain the copper-manganese alloy drug loading system. The copper-manganese alloy drug delivery system selectively releases manganese ions, can widely inhibit expression of cancer cell glycolysis related genes, hinder cancer cell glycolysis, mediate cancer cell pyroptosis and activate anti-tumor immunity, KHK-IN-2 can inhibit cancer-promoting macrophage fructose metabolism, finally macrophage metabolism-immune reprogramming is achieved, and the anti-tumor curative effect is further enhanced.

The utility model discloses a multifunctional massage waistband which comprises a waistband body, a control module, an air bag massage module and a heating module. The waistband main body comprises a waist cone body and a waistband; the control module is arranged in the storage groove; the air bag massage module is arranged in the containing groove. The air bag massage module is selected to massage a user, muscle stress of the user is softer in an air bag expansion mode, the user does not feel uncomfortable, the massage experience of the user is greatly improved, only the heating module is arranged, and the heating part and the massage piece are attached and fixed, so that warm massage enjoyment is achieved for the user, and the user experience is improved. The warming effect can promote muscle relaxation, a better massage effect can be achieved for a user, micro-current pulse signals sent by the control module are transmitted to the human body through the heating part and the massage pieces, muscle contraction and relaxation are stimulated, the muscle capacity is enhanced, muscle fatigue can be relieved, local circulation can be promoted, and cell metabolism can be enhanced.

The invention discloses a tissue regeneration method based on umbilical cord mesenchymal stem cells, and relates to the technical field of stem cell bioengineering and regeneration. Biochemical indexes related to a cell metabolism function and an energy state in an umbilical cord mesenchymal stem cell culture system are collected in real time, time synchronization calibration is carried out with a unified timestamp, and a continuous metabolism characteristic matrix is constructed. According to the method, a multi-parameter metabolism scoring system and a probability model are constructed, the cell metabolism unbalance index is output in real time, laser irradiation parameters are intelligently adjusted according to the index, and dynamic matching of laser energy input and cell metabolism bearing capacity is achieved. Compared with a traditional method, the method has higher physiological adaptability and risk prediction capacity, the stability and consistency of cell amplification are remarkably improved, the cell death rate is reduced, the controllability and safety of the preparation process are enhanced, and a high-quality stem cell preparation scheme is provided for clinical regenerative medicine.

The invention relates to the technical field of prevention and treatment of complications after organ transplantation, and discloses a preparation for preventing and treating diabetes mellitus after organ transplantation based on a Treg cell metabolic regulation function and application, the preparation takes repair or enhancement of the Treg cell metabolic regulation function as a core action mechanism, and comprises at least one active component, according to the PTDM prevention and treatment preparation based on the Treg cell metabolic regulation function and the application, the preparation contains IL-2 / anti-CD3 / CD28 beads, FKBP12siRNA, a PPAR-gamma agonist, SOCS-3siRNA and other active ingredients, and the active ingredients are matched with pharmaceutical auxiliary materials to be prepared into an injection or a freeze-dried powder injection; the preparation is suitable for PTDM high-risk recipients using CNI after kidney / heart transplantation, has a single drug and combination scheme, can also amplify Treg cells in vitro for transfusion to assist intervention, can realize'immune tolerance-metabolic regulation 'double-effect synergy, accurately target PTDM key pathways, adapt to multiple transplantation types, and can be combined with CPB for blood perfusion to synergistically reduce PTDM and AKI risks, reduce non-target injury and improve the curative effect of the PTDM. The life quality of a recipient is improved, the medical burden is reduced, and the blank of PTDM immunotherapy is filled.

The invention discloses a lipid compound for enhancing immune response level and a preparation method and application thereof. The lipid compound provided by the invention has a symmetrical tail carbon chain and comprises a compound as shown in a formula IX. The invention also provides lipid nanoparticles, a drug delivery system and a pharmaceutical composition containing the lipid compound. The lipid compound can promote cell mitochondria activation and enhance the cell metabolism level, so that immune response is remarkably improved, and the lipid compound has wide application prospects in the fields of mRNA vaccines, protein vaccines, CAR-T cell therapy and the like.