131 results about "Mitochondrion membrane" patented technology

The invention belongs to the technical field of bioengineering, and discloses a sunflower seed peptide for regulating metabolism of brain cells, the sunflower seed peptide has a biological sequence of Arg His Ala Lys Thr Pro Ser Asn Lys, and a preparation method of the sunflower seed peptide for regulating metabolism of brain cells comprises the following specific steps: S1, raw material pretreatment; s2, peeling and crushing; s3, protein extraction; s4, performing enzymolysis treatment; s5, performing enzyme deactivation treatment; s6, filtering and purifying; s7, concentrating and drying; and S8, quality detection and packaging. The sunflower seed protein is directionally decomposed into the active peptide fragment by precisely regulating and controlling compound protease and enzymolysis conditions, the active peptide fragment can be compatible with a brain cell specific receptor and penetrate through a blood brain barrier, and cell experiments and animal models prove that the peptide fragment can activate a mitochondrial respiratory chain complex, so that the mitochondrial membrane potential is increased by more than 30%, and the mitochondrial membrane potential is increased by more than 30%. Meanwhile, the activity of neurotransmitter synthetase can be enhanced, the brain health is protected, and tests further prove that the sunflower seed peptide also has the function of regulating the metabolism of brain cells.

The invention relates to the technical field of antitumor drugs, in particular to a Ru (II) complex and a preparation method and application thereof, and the Ru (II) complex has a structure as shown in a formula I. The Ru (II) complex synthesized by the method disclosed by the invention not only has relatively high cytotoxicity, but also has good photodynamic antitumor activity. After the complex is illuminated, the efficiency of the complex entering cells in an active transportation mode can be accelerated, mitochondria and endoplasmic reticulum are targeted at the same time, mitochondrial membrane potential decline and endoplasmic reticulum stress are caused to form a dual organelle damage effect, immunogenic cell death is caused, the tumor microenvironment is adjusted, and the tumor cell immunogenicity is improved. The enrichment of dendritic cells (DCs) in tumor cells is realized, the chemotactic activity of effector T cells is improved, the proportion of CD4 < + > and Foxp3 < + > cell populations is reduced, and finally the anti-tumor immune response is activated. Meanwhile, the complex provided by the invention can also induce the cell to generate pan apoptosis, retard the cell cycle in the G2 / M period and enhance the effect of the complex in inhibiting tumor proliferation.

The invention belongs to the technical field of cosmetics, and discloses a composition for promoting metabolism of hair papilla cells as well as a preparation method and application of the composition. The composition disclosed by the invention is prepared from the following components: a cacumen biotae extract, trehalose, betaine, acetyl tetrapeptide-3 and myristoyl pentapeptide-4. The components in the composition have a good synergistic effect, the mitochondrial membrane potential of the hair papilla cells can be obviously enhanced, the cell activity of the hair papilla cells is promoted, the cell metabolism function of the hair papilla cells is promoted, and therefore the health of hair follicles is effectively maintained.

The invention discloses a modular tumor microenvironment response polypeptide and a preparation method and application thereof. The modular tumor microenvironment response polypeptide is composed of the following functional modules: a targeting and chemotactic module used for targeting tumor cell surface p32 protein and tumor lymphatic vessels and promoting tumor tissue penetration and enrichment; the microenvironment double-response module is used for being cut by MMP-2 / 9 enzyme and broken by high-concentration glutathione GSH in a tumor microenvironment to trigger self-assembly; the self-assembly driving module is used for exposing after the microenvironment responds to activation and driving short peptides to be self-assembled to form a nano structure; a killing module: a pro-apoptotic peptide PAD used for targeting a mitochondrial membrane and inducing tumor cell apoptosis; and the stability optimization module is used for enhancing the degradation resistance of the oligopeptide. By integrating targeted delivery, enzyme digestion response, acid-sensitive release and stabilizing structures, the problems of insufficient targeting property, inaccurate release, poor stability, diagnosis-treatment splitting and the like of the anti-tumor drug and the developing agent are solved.

The invention discloses a nasal delivery multistage targeting bionic nano drug delivery system as well as a preparation method and application of the nasal delivery multistage targeting bionic nano drug delivery system. According to the drug delivery system, a black phosphorus nanosheet with an active oxygen scavenging effect is used as an inner core carrier, a neuroprotective agent fingolimod hydrochloride FTY720 and polydimethyldiguanide PolyMet are loaded, and a microcolloid cell membrane and a mitochondrial membrane are modified on the surface of the preparation; the nano drug delivery system is applied to treatment of cerebral arterial thrombosis reperfusion injury, can overcome the blood brain barrier, can quickly enter the brain through nasal administration, realizes efficient delivery of drugs, remarkably reduces the cerebral infarction area, reduces the brain inflammation level, protects nerve cells, greatly relieves cerebral arterial thrombosis reperfusion injury, and has application prospects.

The invention provides a novel application of a raspberry extract. It is found through tests that any one of isoquercitrin, ellagic acid, vanillic acid, oleanolic acid, kaempferol-3-O-rutinoside, kaempferol-3-O-sophoroside, tiliroside, tormentic acid and raspberry extract can remarkably improve the activity of the human ovarian granular cells or remarkably improve the oxidative stress level, and the activity of the human ovarian granular cells can be remarkably improved. According to the present invention, the ovarian function can be effectively protected by the eight components, the ovarian function can be effectively protected by the eight components, or the mitochondrial membrane potential level can be significantly regulated and controlled, or the estrogen level in the microenvironment can be effectively maintained, such that the ovarian function can be effectively protected by the eight components, and the raspberry extract containing the eight components. The invention provides a new choice for ovarian function protection products, and also provides a new application for the substances.

The invention relates to the technical field of biological medicines, and particularly discloses application of betaine in preparation of a medicine for preventing or treating sepsis myocardial injury. Betaine (BET) provided by the invention can be used for effectively relieving cytokine storm caused by sepsis injury and inhibiting reduction of core body temperature of a mouse; the abnormal blood routine and blood biochemical indexes are improved. In addition, betaine can reduce generation of active oxygen in myocardial cells of the HL-1 mouse and improve mitochondrial membrane potential imbalance of the myocardial cells of the HL-1 mouse. The chemical structural formula of the betaine is shown in the specification.

The invention discloses a preparation method of DRP1 engineered mitochondrial derived vesicles and application of the DRP1 engineered mitochondrial derived vesicles in cell aging resistance. The method comprises the following steps: firstly, constructing a lentiviral vector of an overexpressed DRP1 gene, and transfecting cells to obtain a cell strain of the stable overexpressed DRP1 gene; and culturing the cell strain, collecting a culture solution, and separating and extracting the mitochondrial derived vesicles to obtain the DRP1 engineered mitochondrial derived vesicles DRP1-MDVs rich in mtDNA. The DRP1-MDVs is used for treating senescent cells, the DRP1-MDVs is successfully internalized into senescent cells, the ROS level can be effectively reduced, the mitochondrial mtDNA and ATP content can be improved, the mtDNA mutation rate can be reduced, the mitochondrial membrane potential and the mitochondrial network structure can be recovered, and finally the expression of senescence-related proteins P16 and P21 can be reduced. The obvious mitochondrial function repairing potential and the cell aging improving effect are shown.

This invention relates to the field of cosmetic compositions, and more specifically to a synergistic anti-aging composition involving energy metabolism and autophagy, its preparation method, and its application. The composition comprises sea buckthorn fruit extract, trehalose, and a cosmetically acceptable polyol carrier. This invention pioneers a betaine-trehalose-NADES extraction system, achieving low-temperature, high-efficiency extraction while preserving the heat-sensitive active ingredients of sea buckthorn. Trehalose possesses both extraction and autophagy activation effects. A selective separation strategy achieves a balance between efficacy, compliance, and process simplicity. The green, circular process reduces costs and is environmentally friendly. Through the synergistic effect of sea buckthorn fruit extract and trehalose, the composition enhances mitochondrial membrane potential, increases ATP production, inhibits excessive activation of the mTOR pathway, and promotes LC3-II conversion, thus synergistically combating skin aging from multiple targets including cellular energy metabolism, signaling pathways, and autophagy.

The invention provides application of taurochenodeoxycholic acid in preparation of a medicine for preventing and / or treating Fuchs corneal endothelial dystrophy, and particularly belongs to the technical field of medicine preparation. The invention provides an application of taurochenodeoxycholic acid in preparation of a medicine for preventing and / or treating Fuchs corneal endothelial dystrophy. Test results show that taurochenodeoxycholic acid can realize prevention and / or treatment of corneal endothelial cell injury caused by UVA; the number reduction, activity reduction, oxidative stress, ROS level rise and mitochondrial membrane potential drop of corneal endothelial cells caused by UVA are relieved; mitochondrial biological energy deficiency caused by UVA is relieved; and the prevention and / or treatment of the Fuchs corneal endothelial dystrophy are / is realized by activating a Ca < 2 + > signal channel.

The invention relates to a composition for regulating autophagy of eukaryotic cells and improving mitochondrial dysfunction as well as a preparation method and application of the composition. Comprising the following raw materials in parts by weight: 10-30 parts of broccoli powder, 10-30 parts of wheat germ microcapsule powder, 20-40 parts of raspberry extract, 20-40 parts of banana powder, 2-6 parts of leucine and 0.1-1 part of pyrroloquinoline quinone disodium salt. The invention further provides a preparation method of the broccoli powder, the wheat germ micro-capsule powder, the raspberry extract and the banana powder, and application of the composition of the broccoli powder, the wheat germ micro-capsule powder, the raspberry extract and the banana powder. The composition integrates the effects of the six components, is reasonable in compatibility, safe and effective, and various components activate mitochondria by regulating protein and depending on a PINK1 / Parkin dependent pathway and a PINK1 / Parkin non-dependent pathway; meanwhile, by activating a PGC-1alpha signal channel, mitochondrial DNA replication and new mitochondrial generation are promoted; the mitochondrial membrane potential is stabilized, and the ATP synthesis efficiency is improved; mitochondrial autophagy is synergistically activated, mitochondrial dysfunction is improved, and aging is relieved.

The invention discloses a protein complex for protecting mitochondria from being damaged, a pharmaceutical composition and application. According to the application, the influence of nitrate on mitochondrial injury in in-vivo radiation-induced salivary gland injury and in-vitro human salivary gland cell injury is evaluated, the action mechanism in the mitochondrial injury is further deeply explored, and a result shows that sialic acid transporter interacts with FKBP8, so that the mitochondrial injury can be inhibited, and the mitochondrial injury can be inhibited. In turn, particles are formed and anchored on the outer mitochondrial membrane, thereby maintaining membrane integrity. Therefore, the invention provides a brand new therapeutic target for preventing radiation-induced salivary gland injury and other mitochondrial membrane related diseases.

The invention discloses an application of a vinyl quinoline fluorescent dye in preparation of a reagent or a kit for detecting mitochondria or lysosome. The fluorescent dye does not need to be washed, the probe is used for dyeing mitochondria when the mitochondrial membrane potential is normal, and the probe targets lysosome when the mitochondrial membrane potential is reduced, so that the probe can also be used for observing the mitochondrial membrane potential, the influence of washing on the mitochondrial membrane potential and the damage to cells can be avoided, and the applicability is wider.

The application discloses a tumor-penetrating carrier-free nanomedicine as well as a preparation method and application thereof, and belongs to the field of nanomedicine. The carrier-free nanomedicine can significantly improve water solubility of a water-insoluble compound, has mitochondrion targeting and tumor active penetration capabilities, and under the action of a tertiary amine nitrogen oxygen group, the carrier-free nanomedicine can be mostly transported into mitochondria, which is beneficial to inducing a mitochondrion membrane potential drop and cell apoptosis. The carrier-free nanomedicine also has a good blood long-circulation effect and tumor accumulation capability in a body, and an antitumor effect is better than that of a clinical drug, and the carrier-free nanomedicine also has good biological safety. The preparation method is simple, and can be used for preparation of an antitumor drug.

The invention discloses a nano vesicle with tissue targeting and mitochondrial localization as well as preparation and application of the nano vesicle. The nano-vesicle is a nucleus pulposus cell membrane entrapped with a small molecule mitochondrial membrane stabilizer, and tetra-acetylated N-acetamido mannose is added in the culture process of nucleus pulposus cells, so that a sialic acid derivative with an azide group is expressed on the surface of the nucleus pulposus cell membrane. Therefore, triphenylphosphine cations are modified on the surface of the nucleus pulposus cell membrane through a biological orthogonal reaction. The nano-vesicle can stabilize a mitochondrial membrane structure, improve cell aging-related secretion phenotype release and mitochondrial functions, block mtDNA release and SASP factor generation, realize anti-aging, anti-inflammatory and tissue degeneration intervention, can be used for preparing intervention drugs for tissue degeneration diseases related to mitochondrial membrane damage, and has broad application prospects. Or a drug for regulating mtDNA leakage and SASP release in a cell aging model. Wide application prospects and important clinical transformation values are realized.

The present disclosure is directed to compositions comprising mitochondrial optogenetics-based gene therapies and their methods of use. In some embodiments, a composition described herein comprises an expression vector comprising a first nucleic acid sequence encoding a channelrhodopsin fusion protein and a second nucleic acid sequence encoding a luciferase protein. In some cases, the first nucleic acid sequence and the second nucleic acid sequence are operably linked to an expression control sequence. In some instances, the channelrhodopsin fusion protein comprises a channelrhodopsin protein linked to an inner mitochondrial membrane-mitochondrial localization signal (IMM-MLS). In some implementations, when the expression vector is expressed, the luciferase protein is localized to the cytosol. In some cases, the IMM-MLS comprises a leading sequence from a mitochondrial inner membrane protein selected from ABCB10, ABCB140, Cytochrome C, and renal outer medullary potassium channel (ROMK).

The application discloses Bifidobacterium Pseudocatenulatum and application thereof, and the Bifidobacterium Pseudocatenulatum YSBP01CCTCC NO: M 20232067. Experiments prove that the Bifidobacterium Pseudocatenulatum can enhance the antioxidant capacity of Caenorhabditis elegans, prolong the life of the nematode, improve the activity and the reproductive capacity of the nematode, reduce fat accumulation, improve the mitochondrial membrane potential, and delay the aging of the body. The Bifidobacterium Pseudocatenulatum can also significantly inhibit the weight increase of a high-fat-diet mouse, reduce the TG level in serum of the mouse, significantly reduce the fasting blood glucose concentration, and effectively prevent and relieve obesity and type 2 diabetes.

This invention discloses a method for detecting mitochondrial membrane potential in the gills or hepatopancreas of crustaceans, belonging to the field of mitochondrial membrane potential detection technology. This invention, by addressing the structural and physiological differences between gill and hepatopancreas tissues in crustaceans, formulates differentiated incubation strategies: for gill tissue, a "live pre-equilibration-short-time staining-rapid slide preparation" method is used, strictly controlling the JC-1 incubation time to within 10 minutes to effectively avoid probe toxicity damage to mitochondria; for hepatopancreas tissue, in vivo incubation is extended to 1 hour to ensure sufficient dye penetration. Under the condition of uniformly using 2 μM JC-1 and 20 μmol / L CCCP as positive controls, this method significantly improves the tissue penetration and signal uniformity of the dye, solving problems such as uneven staining and weak signal caused by exoskeleton barriers and high lipid content, providing reliable technical support for aquatic health assessment and toxicology research.

The invention discloses a method for synergistically improving the activity of porcine oocytes through low-temperature plasma and erythritol, and belongs to the technical field of reproductive biology. The method comprises the following steps: collecting pig ovaries and separating oocytes; freezing protection is carried out by adopting a freezing liquid (DMSO: EG: FSF = 2: 2: 6) containing 0.5 mol / L erythritol, and preservation is carried out at-80 DEG C for 48 hours; and after unfreezing, treating for 30 seconds by using low-temperature plasma (6.83 kHz, 4.8 kV) to activate the cells. Experiments show that the erythritol can reduce frozen ice crystal damage, and the plasma treatment can significantly improve the cell activity after resuscitation, reduce the ROS content, increase the GSH reserve and enhance the mitochondrial membrane potential. And the survival rate of the oocytes is improved through synergism of the two. The problems that the cell activity is reduced and oxidative damage is serious in the cryopreservation process are solved, and an efficient technical scheme is provided for preservation of pig germplasm resources.

The application discloses a kind of mitochondrial quality control detection methods, the present application relates to mitochondrial quality control detection technical field, operating steps include preparation sample cell suspension, fluorescent dye labeling, cardiomyocyte separation, establish myocardial cell damaged model and detect mitochondrial membrane potential situation.This mitochondrial quality control detection method can simultaneously carry out multi-parameter, rapid quantitative analysis and sorting, measure fast, measure the multi-parameter characteristics of each cell, while carrying out cell characteristic analysis, can separate out the cell of specified characteristics, so as to further culture, cloning, observation or carry out mitochondrial fragmentation test to specific cell, establish myocardial cell damaged model using doxorubicin, the mitochondrial membrane potential situation of normal myocardial cell and damaged myocardial cell obtained by using JC-1 mitochondrial membrane potential fluorescent probe detection is verified the detection quality of this mitochondrial quality control detection method.

The present application relates to the technical field of retinal ischemia-reperfusion injury, and specifically to RNA, pharmaceutical compositions, conjugates and applications. The nucleotide sequence of the RNA is shown in SEQ ID NO: 1 and / or SEQ ID NO: 2. The RNA can inhibit the proliferation of retinal Müller cells, increase the changes in mitochondrial membrane potential of retinal Müller cells, promote Müller cell apoptosis, reduce Müller cell glial activation and protect ganglion cells from damage, reduce the inflammatory response of retinal ischemia-reperfusion injury, and has application prospects in the prevention and treatment of retinal ischemia-reperfusion injury.

The invention provides a liposome for protecting mitochondrial vesicles of mesenchymal stem cells as well as a preparation method and application of the liposome, and belongs to the technical field of liposome preparation. The liposome comprises a lipid membrane and a mitochondrial protective agent located in the lipid membrane, preparation raw materials of the lipid membrane comprise DOPC, DOTAP, cholesterol and DSPE-PEG2000, and preparation raw materials of the mitochondrial protective agent comprise human serum albumin, trehalose, glutathione, tryptophan and the like. The liposome has good biocompatibility and safety, and immunogenicity and toxicity risks are reduced. After the lipidosome is fused into the mitochondrial vesicles, the structural integrity, the membrane fluidity and the functional activity of mitochondria can be effectively maintained, mitochondrial swelling and collapse are reduced, and the mitochondrial membrane potential is maintained; the liposome vesicles can be stably stored for 6-8 hours at 25 DEG C, and the short-distance transportation requirement is met. The preparation raw materials of the liposome are low in cost, the preparation method is simple and easy to implement, and the liposome is suitable for large-scale production.

The application discloses a method for inducing T cell reprogramming into NK-like cells, comprising the following steps: (1) culturing the T cells in a culture system to obtain activated T cells; (2) adding small molecule inducers twice to the activated T cells to obtain NK-like cells; wherein the culture system in step (1) contains 1-10 v / v % serum substitute and / or platelet lysate, and does not contain fetal bovine serum. By optimizing the culture process and precisely adding drugs twice, the small molecule accumulation toxicity caused by multiple drug additions is avoided, the damage to the structure and function of the mitochondrial membrane is reduced, the mitochondrial membrane potential is significantly improved, the energy metabolism capacity of the mitochondria is enhanced, sufficient energy support is provided for the long-term survival and function maintenance of the cITNK cells, and the physiological activity of the cells is ensured from the energy metabolism level.

The invention relates to the field of biological medicine, in particular to an endoplasmic reticulum targeting peptide, an agonist, a preparation method and application. Wherein the agonist comprises an endoplasmic reticulum targeting peptide, and further comprises a mitochondrial membrane disturbing sequence and a sequence with cathepsin B response, the mitochondrial membrane disturbing sequence is KLAKLAK 2, and the sequence with cathepsin B response is GFLG; the endoplasmic reticulum targeting peptide comprises a KKKAA peptide sequence, and chlorin e6 and R8 peptide are connected to the KKKAA peptide sequence; the mitochondrial membrane disturbing sequence is connected to the R8 peptide through a sequence with cathepsin B response. According to the scheme, the characteristics of biochemical information interaction and tumor growth regulation of the mitochondria and the endoplasmic reticulum are utilized, excessive stress of the endoplasmic reticulum and a mitochondria calcium uptake channel are activated at the same time in a safe and controllable mode, free Ca < 2 + > in the endoplasmic reticulum rapidly flows into the mitochondria, the steady states of the endoplasmic reticulum and the mitochondria of tumor cells are damaged, and the tumor growth is controlled. And the two synergistically induce cells to start an apoptosis procedure, so that the aim of treating diseases is fulfilled.

The invention relates to the technical field of biology, in particular to acoustoelectric Ba0. 85Ca0. 15Ti0. 9Yb0. 1O3 nanoparticles capable of triggering post-translational modification as well as a preparation method and application of the acoustoelectric Ba0. 85Ca0. 15Ti0. 9Yb0.1 O3 nanoparticles. Stirring and mixing the titanium tetrachloride solution and the mixed reagent solution to obtain a stirred mixed solution; mixing the stirred mixed solution with sodium hydroxide and polyvinylpyrrolidone, and performing heat treatment to obtain a heat-treated substance; and carrying out solid-liquid separation on the heat-treated substance to obtain a solid, washing the solid to be neutral, and drying. The nanoparticles provided by the invention consume H < + > near mitochondria under ultrasonic stimulation, so that mitochondrial membrane potential depolarization is caused. After mitochondrial membrane potential depolarization, E3 ubiquitin ligase Parkin is transferred from cytoplasm to a mitochondrial outer membrane, and is massively gathered and accumulated on the mitochondrial outer membrane, so that ubiquitination modification and cascade degradation of mitochondrial outer membrane anti-apoptosis protein MCL-1 are caused, and cell apoptosis is promoted.

The invention belongs to the technical field of biology, and particularly relates to application of astragaloside in regulation of parthenogenetic activation embryo development. According to the method disclosed by the invention, the maturation rate of the oocytes can be obviously improved by adding substances such as astragaloside into the sheep oocyte in-vitro maturation liquid. The preparation method comprises the following steps: firstly, respectively adding lycopene, astragaloside, isoliquiritigenin, forsythin and baicalein into an oocyte maturation solution, and screening out the optimal dosage of each additive according to the first polar body discharge rate, the cleavage rate and the blastocyst rate; and finally, adding an additive combination into the oocyte maturation liquid according to the screened optimal dosage, detecting the first polar body discharge rate, the cleavage rate and the blastocyst rate to determine an optimal composition, and detecting the levels of ROS, GSH, mitochondrial membrane potential, oxidative stress and apoptosis related genes of the optimal composition to further determine the application effect of the composition.

The invention belongs to the technical field of cell biology, and provides an oocyte in-vitro maturation culture solution additive and application thereof. Experimental results show that coumarin 106 can improve the retardation of the meiosis process of oocytes, reduce the form proportion of abnormal spindles of oocytes and the proportion of wrongly arranged chromosomes, recover the level of acetylated tubulin and improve the stability of microtubules; the actin expression level in the spindle body of the oocyte is improved; the conditions of wrong distribution and expression decline of mitochondria in oocytes are improved; the mitochondrial membrane potential level is improved; fluorescence signals of oocyte cortical particle and specific cortical particle protease are improved; the fertilization rate is improved; the active oxygen level and the DNA damage level in the oocytes are reduced, and the occurrence of early apoptosis in the oocytes is reduced. A culture solution prepared from the coumarin 106 can be used for promoting in-vitro maturation of oocytes.

The application discloses a butyl tin-cycloiridium phenanthroline complex with an aggregation-induced emission (AIE) property, a preparation method of the complex and anticancer application of the complex. The structural formula of the complex is shown as formula (I), and R is one of hydrogen, a phenyl group, a p-tolyl group, a p-bromophenyl group, a p-fluorophenyl group, a biphenyl group, a triphenylamine group, a carbazole group, a tetraphenyl ethene group and a triphenylbenzene group. The growth inhibition rates of the target compound on human alveolar basal epithelial carcinoma cells (A549) and cisplatin-resistant cells (A549 / DDP), cervical cancer cells (Hela) and human lung normal epithelial cells (BEAS-2B) are tested, and it is found through comparison that the target compound shows potential anticancer activity. In addition, the target compound shows unique AIE emission characteristics. The target compound mainly targets the lysosomes of A549 cells, and influences the mitochondria to cause the decline of the mitochondrial membrane potential, thereby showing anticancer activity.

The invention discloses a system for monitoring the influence of atorvastatin on mitochondrial functions in real time, and the system comprises a biosensor module which is used for monitoring biomarkers related to the mitochondrial functions and glycometabolism in blood in real time; the micro-fluidic chip is used for rapidly separating and enriching cells or specific components in blood; the optical detection module monitors the active oxygen level of mitochondria in real time; the data processing and transmission module processes and analyzes the collected data in real time; the equipment terminal receives the processed data and displays the data on a user interface in real time; the analysis and early warning module analyzes the monitoring data in real time and provides early warning and personalized suggestions for diabetes mellitus; the database module is used for storing monitoring data and historical records; through a biosensor, optical detection, a micro-fluidic chip and the like, after a patient takes atorvastatin, indexes of blood sugar, insulin and the like in a blood sample, and mitochondrial functions and oxidative damage indexes of active oxygen level, mitochondrial membrane potential and the like are detected.

The application discloses a kind of mitochondrial fluorescent probe independent of mitochondrial membrane potential and its preparation method and application, the mitochondrial fluorescent probe independent of mitochondrial membrane potential provided by the application can target and image mitochondria when mitochondrial membrane potential decreases and disappears.