Novel application of ginkgetin

A technology of ginkgo biflavones and biflavonoids, which is applied in the field of medicine, can solve the problems of non-small cell lung cancer research that has not been reported, and the research on the antitumor efficacy of ginkgo biflavones is rare.

Active Publication Date: 2016-11-30
THE HONG KONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Ginkgo biloba is rich in biflavonoids, among which Ginkgo biloba L. is the dry leaf extract of Ginkgo biloba L., and its efficacy has been mainly focused on the treatment of coronary heart disease, angina pectoris, and cerebral arteriosclerosis. , senile dementia, hypertension, there are few studies on the antitumor efficacy of ginkgo biloba, especially for non-small cell lung cancer.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: The Toxic Effect of Ginkgo biloba Flavonoids on A549 Non-small Cell Lung Cancer Cells

[0032] 1. Cell culture

[0033] Get A549 cells and cultivate them in 1640 medium, which contains 10% fetal bovine serum, penicillin 100U / ml, gentamicin 100ug / ml, 1mM amino acid and 0.1mM sodium pyruvate, and the temperature of the incubator is 37 °C, with 5% CO 2 .

[0034] 2. Cytotoxicity Assay

[0035] A549 cells were planted in a 96-well plate with a cell density of about 3×10 per well 3 After 24 hours of cell attachment, give different concentrations of ginkgo biloba and cisplatin, add MTT staining after 24 hours, 48 ​​hours, and 72 hours, and discard the original culture medium after 4 hours of staining, add DMSO to dissolve, and put it into a microplate reader , the absorbance was measured using a detection wavelength of 570 nm.

[0036] For comparison results, see the attached figure 1 and Table 1. The results showed that ginkgobiflavone had strong cytotoxic ...

Embodiment 2

[0039] Example 2: Ginkgo Biflavone Increases the Mitochondrial Membrane Potential of A549 Cells

[0040] A549 cells were planted on a 6-well plate with a cell density of about 5×10 per well 4 Let the cells adhere to the wall overnight, add different concentrations of Ginkgo biloba (5, 10 μM), and replace the culture medium before adding the medicine. 24 hours after adding the drug, discard the medium, wash twice with PBS, digest and collect the cells, add serum-free culture containing JC-1 with a final concentration of 5 μg / μL, and label with fluorescence at 37°C for 0.5 hours, centrifuge to discard the medium, wash with PBS Twice, resuspended and detected by flow cytometry, the excitation wavelengths are 488nm and 561nm.

[0041] For comparison results, see the attached figure 2 . The results showed that Ginkgo biloba could significantly increase the reduction rate of mitochondrial membrane potential in A549 cells, which was significantly higher than that of cisplatin at ...

Embodiment 3

[0042] Example 3: Ginkgo biloba increased the production of reactive oxygen species (ROS) in A549 cells

[0043] A549 cells were planted on a 6-well plate with a cell density of about 5×10 per well 4 Let the cells adhere to the wall overnight, add different concentrations of Ginkgo biloba (5, 10 μM), and replace the culture medium before adding the medicine. 24 hours after the addition of the drug, add serum-free culture containing DCFH-DA with a final concentration of 15 μM based on fluorescent labeling at 37°C for 0.5 hours, then discard the medium, wash twice with PBS, digest and collect cells, and use flow cytometry for detection. The detection excitation wavelength is 488nm. The experimental results are the average value of three experiments, and three holes are made in parallel each time.

[0044] For comparison results, see the attached image 3, the results showed that: Ginkgo biloba can significantly increase the production of ROS in A549 cells, and it was signific...

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Abstract

The invention provides a novel application of a biflavone compound. A biflavone compound (particularly ginkgetin) has a function of preventing and treating lung cancer (especially non-small cell lung cancer). Experiment results show that the ginkgetin has a high cytotoxic effect on A549 non-small cell lung cancer, is capable of obviously increasing the reduction rate of mitochondrial membrane potential and generation of reactive oxygen species, increasing expression of apoptosis-related protein and autophagy critical protein, inducing apoptosis of the A549 non-small cell lung cancer, and obviously inhibiting tumor growth of an A549 nude mouse bearing the tumor, has good antitumor activity for the A549 nude mouse bearing the tumor and is free of obvious toxicity, and through combined application of the ginkgetin and cisplatin that is a common clinical lung cancer treating medicine, anticancer effects of the cisplatin and the ginkgetin during single using are increased and toxicity of the cisplatin is reduced. The biflavone compound (particularly the ginkgetin) can be used for preparing medicines or healthcare products preventing and treating the lung cancer (especially the non-small cell lung cancer).

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of ginkgo biloba biloba in the preparation of medicines for preventing and treating non-small cell lung cancer. Background technique [0002] The high morbidity and mortality of lung cancer make it the main factor affecting the death of tumor diseases. Due to environmental pollution and people's fast-paced lifestyle, the morbidity and mortality of lung cancer show an increasing trend year by year. According to IACR estimates, until 2030, 10 million people will die of lung cancer every year, which is equivalent to 2,700 people dying of lung cancer every day. Lung cancer is mainly divided into non-small cell lung cancer (NSCLC) and small cell carcinoma (SCLC), among which non-small cell lung cancer accounts for more than 80% of lung cancer types. Non-small cell lung cancer can be divided into adenocarcinoma, squamous cell carcinoma, and large cell carcinoma accord...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61K33/24A61P35/00A23L33/105A23L33/16
CPCA23V2002/00A61K31/352A61K33/24A61K2300/00A23V2200/308A23V2200/314A23V2250/1622A23V2250/2116A23V2250/2122
Inventor 詹华强董婷霞楼剑书
Owner THE HONG KONG UNIV OF SCI & TECH
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