32 results about "Biflavonoids" patented technology

The invention discloses a selaginella tamariscina extract, a selaginella tamariscina clathrate compound, selaginella tamariscina formula granules and a preparation method thereof, and relates to the field of medicines. The invention provides a preparation method of a selaginella tamariscina extract, which comprises the following steps: adding 12-16 times of water into selaginella tamariscina, soaking, decocting and distilling for the first time, and collecting first volatile oil and dregs; adding 8-10 times of water into the medicine residues, performing secondary decoction and distillation, and collecting second volatile oil and decoction liquid; combining the first volatile oil and the second volatile oil. The preparation process can effectively extract the active ingredients in the selaginella tamariscina, the active ingredients are applied to preparation of the selaginella tamariscina formula granules, the content and transfer rate of volatile oil in the selaginella tamariscina formula granules and the extraction rate of amentoflavone can be remarkably increased, so that the clinical effectiveness of the selaginella tamariscina formula granules is improved, and the selaginella tamariscina formula granules have the advantages of being high in stability, simple to operate, green, environmentally friendly and the like; and a way is provided for large-batch production of the selaginella tamariscina formula granules.

The invention discloses application of ginkgetin in treatment of chronic pulmonary obstruction, and relates to the field of medical technical methods of ginkgetin. According to the invention, by constructing a model of cigarette extract on airway epithelial cell injury, the biflavone compound is found to have a protective effect on epithelial cell injury caused by smoking; through transcriptomics research, biflavone compounds such as ginkgetin and the like are found to have pharmacological activity of inhibiting CEBP beta protein high expression caused by cigarette extracts, so that the effect of protecting airway inflammation caused by smoking is achieved. More importantly, the ginkgetin and the glucocorticoid are jointly used, so that the treatment effect on chronic pulmonary obstruction is remarkably improved compared with that of independently using the ginkgetin and the glucocorticoid, and the ginkgetin and the glucocorticoid have wide medical application.

The invention provides an application of ginkgetin in enhancing VS4718 or ponatinib in resisting the progress of esophageal squamous cell carcinoma. It is found that ginkgo biflavone can enhance the growth and invasion inhibition effect of VS4718 or ponatinib on esophageal squamous carcinoma cells; ginkgo biflavone is used for enhancing the inhibition effect of VS4718 or ponatinib on glycolipid metabolism of tumor cells, and the ginkgo biflavone is respectively combined with VS4718 or ponatinib to generate a synergistic anti-cancer effect.

The invention belongs to the technical field of medicines, and particularly relates to application of ginkgetin in preparation of a medicine for delaying senescence of mesenchymal stem cells. The application of the ginkgo biflavone in delaying the aging of the adipose-derived stem cells is explored by exploring the effects of a culture system added with the ginkgo biflavone in delaying the aging process of the adipose-derived stem cells and maintaining the inherent attributes of the adipose-derived stem cells, and a theoretical basis and a technical thought are provided for related intervention of aging and obesity and treatment of related diseases.

The invention discloses a novel application of ginkgo biflavone in lung cancer treatment, belongs to the technical field of medicines, and researches show that ginkgo biflavone can target CDC7 and is a novel targeted medicine. It is confirmed for the first time that ginkgo biflavone regulates and controls the thermal stability and enzymatic degradation of ginkgo biflavone through targeting CDC7 kinase (binding free energies-8.3 kcal / mol), directly intervenes in the DNA replication process, and provides a natural drug lead compound for development of a novel CDC7 small-molecule inhibitor. The compound has great significance in preventing and treating malignant tumors, and has a wide prospect in the aspect of preparing medicines for treating lung tumors.

The application provides a method for separating biflavonoids from Rhus potaninii flower buds, which specifically comprises the following steps: firstly, taking Rhus potaninii flower bud powder, and refluxing extraction by 95% ethanol, and filtering to obtain a filtrate; then purifying the filtrate by semi-preparative HPLC to obtain an eluate; after detecting the eluate at 300 nm, the eluate with a retention time of 20.5-23.0 min and 38.4-40.6 min is collected; and the eluate is concentrated and evaporated under reduced pressure to obtain biflavonoids Rhusflavanone and Rhusflavone. The separation method is simple, fast, efficient, environmentally friendly, and the obtained biflavonoids have high purity. The obtained biflavonoids can be used as a control sample to determine the content of the biflavonoids in Rhus potaninii and its pseudo-products Rhus succedaneum and Rhus succedaneum, and effectively identify whether the pseudo-products are replaced in the medicinal material or the preparation, and fill the technical blank.

The invention provides an application of ginkgetin in enhancing VS4718 or ponatinib in resisting the progress of esophageal squamous cell carcinoma. It is found that ginkgo biflavone can enhance the growth and invasion inhibition effect of VS4718 or ponatinib on esophageal squamous carcinoma cells; ginkgo biflavone is used for enhancing the inhibition effect of VS4718 or ponatinib on glycolipid metabolism of tumor cells, and the ginkgo biflavone is respectively combined with VS4718 or ponatinib to generate a synergistic anti-cancer effect.

The invention relates to the field of material science and engineering, and particularly discloses a screening method of an anti-macrobrachium rosenbergii iridovirus natural compound based on an MCP protein target. The method comprises the following steps: constructing a three-dimensional structure of a virus major capsid protein MCP and determining an active pocket; performing structure pretreatment on the natural compound library; by taking the active pocket as a target spot, performing multiple rounds of virtual screening through molecular docking to obtain a candidate compound with binding energy less than or equal to-10.0 kcal / mol; and establishing a macrobrachium rosenbergii artificial infection model, determining the virus inhibition rate and protection rate of the candidate compound, and screening the active compound. The eugenol, the amentoflavone, the polyphyllin II and the solamargine screened by the method show a remarkable antiviral effect in an in-vivo injection test and breeding verification. According to the method, accurate screening from target spot prediction to in-vivo verification is realized.

The application discloses active components in Selaginella lepidophylla and application of the active components in inhibition of alpha-glucosidase. The active components comprise four sesquiterpenes, three lignans and three biflavones; wherein, compound 1 is a new sesquiterpene compound, and compounds 2-10 are obtained from Selaginella lepidophylla for the first time. The compounds obtained by separation are subjected to in-vitro enzyme inhibition activity evaluation, so as to lay a scientific foundation for reasonable development and utilization of Selaginella lepidophylla plant resources. The alpha-glucosidase inhibition activity test result shows that compounds 7-10 have good alpha-glucosidase inhibition activity.

The present invention relates to the field of cosmetic technology, and in particular to a composition with water-oil harmonizing effect and its application. The composition with water-oil harmonizing effect comprises 0.5 to 3 parts of red pine lactone and 1 to 10 parts of schizonepeta biflavonoids by weight. The composition of the present invention, by using the two components of red pine lactone and schizonepeta biflavonoids in combination, has a synergistic effect, can significantly improve the regulating effect on oils and fats, and achieve the effect of water-oil harmonizing; the composition of the present invention can be used as a raw material for water-oil harmonizing effect in cosmetic products, and can significantly improve the water-oil harmonizing effect of cosmetic products.

The invention relates to the technical field of medicines, in particular to a compound composition for preventing alopecia and promoting hair growth and application thereof. The invention provides a compound composition for preventing hair loss and promoting hair growth. The compound composition is prepared from the following raw materials in parts by weight: 1-5 parts of amentoflavone and 1-10 parts of unodil. The invention provides an application of amentoflavone and unodil in hair loss prevention and hair growth. An in-vitro cell experiment shows that after the amentoflavone and the unodil are combined, the proliferation of human primary dermal papilla cells can be promoted. A preclinical animal experiment shows that after the amentoflavone and the unodil are locally used, the hair growth speed is increased, and the hair density is increased.

The invention belongs to the field of pharmaceutical chemicals, and relates to a biflavone compound as well as an extraction method and application thereof. The structural formula of the biflavone compound is as shown in formula 1: # imgabs0. The high-purity C-O-C bonding type biflavone compound is prepared through a series of steps of crushing, extraction, concentration, extraction, column chromatography, HPLC (High Performance Liquid Chromatography), drying and the like, and the anti-HCC activity of the compound is superior to that of reported C-C bond type amentotaxus biflavone and C-O-C type hinokiflavone; therefore, the compound has higher potential for preparing the hepatocellular carcinoma medicine.

The invention discloses biflavone oxygen-methyltransferase as well as a coding gene and application thereof. The biflavone oxygen-methyltransferase has an amino acid sequence as shown in any one of SEQ ID NO: 1-5, or the amino acid sequence of the biflavone oxygen-methyltransferase and the amino acid sequence as shown in any one of SEQ ID NO: 1-5 have at least 95% of sequence identity. The biflavone oxymethyltransferase provides a new technical path for large-scale production of amentotaxus biflavone compounds, has important application value, not only provides an important gene element for biosynthesis of amentotaxus biflavone compounds with high pharmacological activity, such as bilobaliflavone, ginkgetin and isoginkgetin, but also provides an important gene element for biosynthesis of amentotaxus biflavone compounds with high pharmacological activity, such as bilobalitin, ginkgetin and isoginkgetin. The method has reference significance in widening production tools and application of biflavone compounds.

The invention relates to the technical field of medicines, and finds that a natural product amentoflavone can be used as a GPR35 (G-protein coupled receptor 35, GPR35) receptor agonist and application thereof, the compound is amentoflavone, has GPR35 receptor agonist activity, is a GPR35 receptor agonist, can be used for treating thromboembolic diseases, spring conjunctivitis, intracranial embolism, primary sclerosing cholangitis and myocardial infarction, and can be used for treating various diseases such as thromboembolic diseases, spring conjunctivitis, intracranial embolism, primary sclerosing cholangitis and myocardial infarction. Therefore, a novel GPR35 receptor stimulant seedling compound with a clear action target can be provided for the related diseases.

The invention provides a method for separating biflavone compounds from ferrugina flower buds, which specifically comprises the following steps: firstly, taking ferrugina flower bud powder, adding 95% ethanol for reflux extraction, and filtering to obtain filtrate; the preparation method comprises the following steps: firstly, filtering the raw materials to obtain a filtrate, then purifying the filtrate through semi-preparative HPLC (High Performance Liquid Chromatography) to obtain an eluent, detecting the eluent at 300nm, determining the eluent with the collection and retention time of 20.5-23.0 minutes and 38.4-40.6 minutes, and carrying out vacuum concentration and evaporation to dryness to obtain the biflavone compounds Rhusflavanone and Rhusflavanone. The separation method disclosed by the invention is simple, rapid, efficient and environment-friendly, and the obtained biflavone compound is high in purity. The biflavone compound obtained by the invention can be used as a reference substance, is used for measuring the content of the biflavone compound in the fermentum and counterfeit siamensis siamensis and siamensis siamensis and siamensis siamensis and siamensis siamensis, and effectively identifying the authenticity of a fermentum medicinal material or whether counterfeit replacement exists in a preparation or not, so that the technical blank is filled.

The invention relates to the technical field of medicinal compounds, and particularly discloses a biflavone compound and a preparation method and application thereof.The preparation method comprises the steps that suaeda salsa is taken and cut into small sections, the small sections are soaked in methyl alcohol, extracting solutions are mixed, and the mixed extracting solutions are concentrated into fluid extract to obtain total extract; the preparation method comprises the following steps: sequentially adding diluted hydrochloric acid to adjust the pH value to 3-5, extracting with ethyl acetate, adjusting the pH value to 11-13 with a sodium hydroxide solution, extracting with ethyl acetate, combining extract liquor, concentrating under reduced pressure to remove ethyl acetate to obtain a crude extract, and sequentially performing ODS column chromatography chopping and silica gel column chromatography elution on the crude extract to obtain the compound. According to the method disclosed by the invention, a novel biflavone compound can be extracted and separated from suaeda salsa, and the compound has anti-gastric cancer and antibacterial activity. The biflavone compound extracted and separated by the invention has proliferation inhibition activity on a human gastric cancer 7901 cell strain. According to the present invention, the biflavone compound obtained through extraction and separation has an inhibition effect on Pseudomonas aeruginosa (Pseudomonas aeruginosa)-ATCC27853.

The invention discloses a nucleic acid construct, an amentoflavone production strain as well as a construction method and application of the amentoflavone production strain. The nucleic acid construct comprises nucleic acid for coding amentotaxus biflavone synthase and nucleic acid for coding a cytochrome P450 enzyme redox partner; the amentotaxus biflavone synthase has an amino acid sequence as shown in SEQ ID NO: 9. According to the invention, the amentotaxus biflavone production strain is established through a gene recombination technology, and biflavone fermentation production and manufacturing by using a heterologous artificial chassis are constructed for the first time. The prokaryotic production strain established by the invention is of great significance to development of novel drugs for treating neurodegenerative diseases and cardiovascular systemic diseases, and a new way is provided for industrial production of the potential drug amentotaxus biflavone.

The embodiment of the invention discloses a selaginella tamariscina extract purification method, a skin care composition and application of a purified product. A specific embodiment of the method comprises the following steps: mixing a selaginella tamariscina extract with a first hydrochloric acid solution to obtain a first mixture; after standing the first mixture for a first time, separating the first mixture to obtain a first precipitate and a first filtrate; mixing the first filtrate with a second hydrochloric acid solution to obtain a second mixture; after standing the second mixture for a second time, separating the second mixture to obtain a second precipitate and a second filtrate; and drying and grinding the second precipitate to obtain a selaginella tamariscina purified product. According to the embodiment, the content of flavonoid chemical components in the selaginella tamariscina extract, especially the content of amentotaxus biflavone, can be further increased, and compared with the prior art, the method has higher amentotaxus biflavone process transfer rate.

The invention relates to a preparation method of amentoflavone from a selaginella medicinal material. The preparation method comprises the following steps: medicinal material extraction, macroporous adsorption resin column chromatography and solid-liquid extraction. According to the characteristic that the amentotaxus biflavone has an acidic phenolic hydroxyl group, the extract rich in the amentotaxus biflavone is obtained by utilizing an alkali extraction and acid precipitation extraction method, then the extract is purified by combining a macroporous adsorption resin process with a solid-liquid extraction process, finally the amentotaxus biflavone with the purity of 98% or above is obtained, and the yield can reach 1.319%. The method reduces the use of an organic solvent, greatly simplifies the extraction, separation and purification process, is easy to amplify production, and has the advantages of simplicity in operation, low cost and high yield.

This invention discloses a novel biflavonoid compound, its extraction method, and its uses. The extraction method is as follows: extraction with an aqueous ethanol solution, concentration to obtain an extract, followed by extraction with ethyl acetate to obtain an extract. The extract is then subjected to one normal-phase column chromatography separation and one reverse-phase column chromatography separation to obtain a crude product. The crude product is further purified by gel column chromatography to obtain the novel biflavonoid compound. Pharmacological studies have shown that this compound has effective anti-inflammatory properties and is suitable for preparing new drugs for the prevention and treatment of inflammation, possessing broad application prospects and significant potential value.

The invention discloses an extraction method of ginkgo biflavone as well as a product and application thereof, belongs to the technical field of small molecule extraction, and aims to solve the technical problem that the purity and the processability of ginkgo biflavone need to be further improved in the prior art. Phenylboronic acid cured silica gel selective adsorption, L-arginine molecular compounding and double-layer solubilizing auxiliary material construction are used as a core technical path, efficient conversion of the whole process from extraction and purification of ginkgetin to tablet forming is achieved, impurities are removed through specific adsorption, dispersity is improved through molecular compounding, and the bioavailability of ginkgetin is improved. The drug release and the structure stability are jointly regulated and controlled by a quick disintegration solubilizing system and a stable solubilizing system, the obtained tablet is high in purity, quick in disintegration, sufficient in dissolution and excellent in long-term storage performance, and the processability and the bioavailability of the ginkgetin in a solid preparation are comprehensively improved.

This invention relates to natural extracts, specifically to the use of a *Selaginella uncinata* extract in the preparation of medicaments for the treatment and / or prevention of phosphodiesterase type 4 (PDE4)-related diseases. The extract of this invention and the biflavonoids therein possess significant PDE4 inhibitory activity and can be used to prepare PDE4 inhibitors for the treatment and / or prevention of PDE4-related diseases.

The invention belongs to the technical field of plant extraction, and discloses a cacumen biotae extract as well as a preparation method and application thereof. The preparation method of the cacumen biotae extract comprises the following steps: mixing cacumen biotae and an extraction solvent, and performing high-pressure homogenization to obtain a cacumen biotae homogeneous solution; heating, extracting and filtering the Chinese arborvitae twig and leaf homogenized liquid to obtain a Chinese arborvitae twig and leaf extract; the extraction solvent comprises a eutectic solvent and water; the eutectic solvent comprises a hydrogen bond acceptor and a hydrogen bond donor, the hydrogen bond acceptor comprises urea and / or betaine, and the hydrogen bond donor comprises sorbitol and / or trehalose. According to the preparation method, the specific deep-eutectic solvent is selected, and a high-pressure homogenization green extraction process is matched, so that active ingredients in the cacumen biotae can be fully released and dissolved, the utilization efficiency of the raw materials is improved, and the prepared cacumen biotae extract contains high-content total flavonoids, myricetin, isoquercitrin, quercitrin, amentoflavone and quercetin; therefore, the traditional Chinese medicine composition has remarkable anti-oxidation and anti-inflammatory effects, and is mild and non-irritant.

The invention belongs to the field of traditional Chinese medicine preparation, and relates to application of biflavone compounds isochamaejasmine and genkwanol A in preparation of anti-complement drugs. According to the present invention, the biflavone compounds such as the isochamaejasme and the genkwanol A are extracted from the ethyl acetate extraction part of the ethanol extract of the dried root of Stellera tianshanica plant Stellera tianshanica Pod. The in-vitro anti-complement activity evaluation experiment results prove that the biflavone compounds such as the isochamaejasme and the genkwanol A have strong inhibition effects on the classical pathway and the alternative pathway of the complement system, wherein the stellera tianshanica and the genkwanol A are extracted from the ethyl acetate extraction part of the ethanol extract of the dried root of Stellera tianshanica Pod. The biflavone compound isochamaejasmine and genkwanol A can be further used for preparing medicines for treating complement related diseases, and the complement related diseases comprise systemic lupus erythematosus, rheumatoid arthritis or acute respiratory distress syndrome.

The present invention belongs to the technical field of traditional Chinese medicine detection, and particularly relates to a method for determining the contents of multiple components in Wuweiganlu medicinal bath powder. The method uses high performance liquid chromatography to simultaneously determine the contents of the main active components hyperoside, luteoloside, quercitrin, quercetin, and amentoflavone in Wuweiganlu medicinal bath powder. The present invention can effectively shorten the analysis time of the main active components in Wuweiganlu medicinal bath powder, has a good linear range, high accuracy, precision, and repeatability, the method is stable and feasible, and can be applied to the quality inspection of products.

The application belongs to the field of medicine and chemical industry, and relates to a biflavonoid compound and an extraction method and application thereof. A structural formula of the biflavonoid compound is as shown in formula 1: The biflavonoid compound is prepared through a series of steps such as crushing, extraction, concentration, extraction, column chromatography, HPLC and drying, and has high purity. The anti-HCC activity of the C-O-C bonded biflavonoid compound is better than that of reported C-C bonded amentoflavone and C-O-C bonded taxifolin, and therefore the biflavonoid compound has high potential for preparing hepatocellular carcinoma drugs.

The present invention provides a use of ginkgo biflavonoids for preparing a product for preventing and / or treating SVA infection; in another aspect, the present invention also provides a product for preventing and / or treating SVA infection, wherein the product contains ginkgo biflavonoids at a pharmacologically effective concentration. The present invention provides the use of ginkgo biflavonoids in preparing a preparation for preventing and / or treating SVA infection. Ginkgo biflavonoids has a very significant effect on preventing SVA infection, can reduce cell infection caused by SVA, and inhibit the adsorption, replication, assembly and release stages of SVA, providing a scientific and reliable theoretical basis for the clinical treatment of swine Seneca virus disease.

The application discloses a ginkgo biloba biflavonoid extraction method and products and applications thereof, belongs to the technical field of small molecule extraction, and is used for solving the technical problem that the purity and processability of ginkgo biloba biflavonoid need to be further improved in the prior art. The application takes the selective adsorption of phenylboronic acid solidified silica gel, the molecular complexing of L-arginine, and the construction of a double-layer solubilization auxiliary material as core technical paths, realizes efficient conversion of the whole process from ginkgo biloba biflavonoid extraction and purification to tablet forming, removes impurities through specific adsorption, improves dispersibility through molecular complexing, and jointly controls drug release and structure stability through a rapid disintegration solubilization system and a stable solubilization system. The obtained tablet has high purity, fast disintegration, sufficient dissolution, and excellent long-term storage performance, and comprehensively improves the processability and bioavailability of ginkgo biloba biflavonoid in solid preparations.

According to the application of amentotaxus biflavone in preparation of the drug for the sensitivity of the bladder cancer to the cis-platinum, the amentotaxus biflavone synergistically improves the activity and proliferation of the cis-platinum in inhibiting bladder cancer T24 cells and bladder cancer 5637 cells through the specific cytotoxic effect of the amentotaxus biflavone on the T24 cells and the 5637 cells, so that the sensitivity of the bladder cancer to the cis-platinum is enhanced, and the sensitivity of the bladder cancer to the cis-platinum is improved. The compound is used for preparing medicines for treating bladder cancer and belongs to the field of medicines.

The invention provides application of ginkgetin in preparation of a medicine for treating osteoporotic diseases, and belongs to the technical field of medicines, according to the regulation and control effect of ginkgetin on bone loss caused by overactivity of osteoclasts, firstly, it is found that ginkgetin inhibits formation of RANKL-induced osteoclasts through TRAP staining; bone resorption experiments and oxyntobutin discover that the biflavone inhibits the bone resorption and oxyntobutin functions of osteoclasts. Through PCR detection, it is found that ginkgo biflavone inhibits expression of specific genes of osteoclast differentiation. Meanwhile, according to transcriptome sequencing analysis and WB experiment clarification of a basic molecular mechanism, it is found that ginkgo biflavone inhibits generation and differentiation of osteoclasts by regulating and controlling streak OXPHOS. In addition, a femoral artery ligation mouse model is constructed to simulate occurrence of hypoxia osteoporosis, and Hamp is scanned through CT; according to E staining and TRAP staining, the bone destruction degree and the number of osteoclasts are analyzed, and compared with FAL group mice, the ginkgo biflavone treatment group obviously reduces the number of osteoclasts in bone tissues.