75 results about "Biflavonoids" patented technology

The invention relates to botanical agricultural bacteriostat used for controlling plant pathogenic fungi, a preparation method and purposes thereof. The botanical agricultural bacteriostat is prepared by the method that stellera chamaejasme is extracted chemically, mixed with the active ingredient including vegetal biflavonoids eluted by 30 percent of ethanol through macroporous absorbent resin RP-8, and then mixed with surface active agent, emulsifier, penetrant and synergistic agent. The characteristics of the agricultural bacteriostat of stellera chamaejasme vegetal biflavonoids are of high effect, low toxicity, less residue and harmoniousness with environment.

The invention discloses a method for extracting hinokiflavone from Cacumen Biotae. The method comprises the following steps: 1) pulverizing Cacumen Biotae, adding 5-10 times of saturated lime water for immersion and extraction for 2-3 times, ultrafiltering the extracting solution through an ultrafiltration membrane, and concentrating the filtrate with a nanofiltration membrane to obtain a concentrated solution; 2) regulating the pH value of the concentrated solution to 3-5, adding into a macroporous resin column for adsorption, carrying out gradient elution with an ethanol solution, concentrating the eluate, regulating the pH value to 3-5, and crystallizing; and 3) dissolving the filtered crystal in a 50-80% methanol solution, concentrating until the methanol concentration is 10-30%, adding into a macroporous resin for adsorption, carrying out gradient elution with an ethanol solution, concentrating the eluate for crystallization, recrystallizing the crystal with a 70% ethanol solution, and drying. The method for producing hinokiflavone has the advantages of simple technological operation, low energy consumption and small pollution, and can easily implement industrial production.

S. pvlvinata (Hook.et Gev.) Maxim of Selaginella of Selaginellaceae, S. tamariscina (beav.) spring, S. moellendorfii Hieron and so on are taken as raw materials for extraction and separation of total flavonoids of the Selaginella and the single component amentoflavone thereof, thus preparing a pharmaceutical preparation for prevention and treatment for Alzheimer disease.

The present invention relates to an improved multi-step method for preparing an extract from ginkgo biloba having a reduced content of 4'-O-methyl pyridoxine and/or biflavones, wherein the depletion is carried out by filtration over an adsorber resin and/or an ion exchanger and the substances to be removed are retained on the resin. The invention further relates to an extract from ginkgo biloba having a reduced content of 4'-O-methyl pyridoxine and/or biflavones, which is obtainable by the method according to the present invention, as well as to its use.

In the invention, methods such as ethyl alcohol extraction and polyamide column chromatography and the like are employed to prepare high content selaginella general flavone and high purity amentotaxus biflavone monomer ingredients in large quantity from S. pvlvinata (Hook. et Gev.) Maxim, S. tamariscina (beav.) spring and S. moellendorfii Hieron of Selaginella for the first time. The selaginella general flavone and the amentotaxus biflavone monomer ingredients are proven to have the function of myocardial ischemia resistance and the new usage of curing angiocardiopathy caused by myocardial ischemia and the like.

The invention provides a novel application of a biflavone compound. A biflavone compound (particularly ginkgetin) has a function of preventing and treating lung cancer (especially non-small cell lung cancer). Experiment results show that the ginkgetin has a high cytotoxic effect on A549 non-small cell lung cancer, is capable of obviously increasing the reduction rate of mitochondrial membrane potential and generation of reactive oxygen species, increasing expression of apoptosis-related protein and autophagy critical protein, inducing apoptosis of the A549 non-small cell lung cancer, and obviously inhibiting tumor growth of an A549 nude mouse bearing the tumor, has good antitumor activity for the A549 nude mouse bearing the tumor and is free of obvious toxicity, and through combined application of the ginkgetin and cisplatin that is a common clinical lung cancer treating medicine, anticancer effects of the cisplatin and the ginkgetin during single using are increased and toxicity of the cisplatin is reduced. The biflavone compound (particularly the ginkgetin) can be used for preparing medicines or healthcare products preventing and treating the lung cancer (especially the non-small cell lung cancer).

The invention relates to high-content S.tamariscina(beav.)spring total flavonoids and a high-purity amentotaxus argotaenia biflavone monomer composition firstly prepared from Selaginella S.pvlvinata(Hook.et Gev.)Maxim, S.tamariscina(beav.) spring and S.moellendorfii Hieron by a macroporous resin and polyamide serial columns in series chromatography method. The S.tamariscina(beav.)spring total flavonoids and the amentotaxus argotaenia biflavone monomer composition are firstly proved to have functions of inhibiting alpha-glucosaccharase, reducing blood sugar, and regulating blood fat, and the S.tamariscina(beav.)spring total flavonoids and amentotaxus argotaenia biflavone in preventing have new application in treating hyperglycemia and hyperlipemia.

The invention discloses a preparation method and application of 3'8''-bis-apigenin type bis-flavonoid. The preparation method of the compound comprises the following steps of: hot refluxing and extracting coarse powders of Taxus cuspidatea twigs and leaves by using ethanol to obtain total extract; and performing reversed phase and normal phase column chromatography, gel and recrystallization and separation on the total extract to obtain the 3'8''-bis-apigenin type bis-flavonoid. The 3'8''-bis-apigenin type bis-flavonoid is used for preparing medicaments for inhibiting formation of senile plaques or treating an Alzheimer disease, can effectively reduce the generation of Beta amyloid, also depolymerizes the aggregated Beta amyloid, has no toxic effect on cells, and also can stimulate growth of cells at a high concentration; and the preparation method has simple process and high yield.

The invention belongs to the technical field of chemical analysis and detection and particularly relates to a method for measuring active ingredient content in Ginkgo biloba L. leaves. According to the method, rapid online optimization is carried out on mass spectrometry parameters comprising a fragmentation voltage and collision energy in an electrospray anion tandem mass spectrometry multiple reaction monitoring (MRM) mode through application of an ultra performance liquid chromatography-tandem quadrupole mass spectrometer, and high-strength target ion pairs are formed. Through utilization of the target ion pairs with the series of characteristics, rapid qualitative and quantitative analysis is carried out on three kinds of active ingredients of ginkgolides, flavonol glycosides and biflavones in a ginkgo biloba medicinal material. The detection method provided by the invention is simple in operation and has high sensitivity and precision, good reproducibility, high stability and a high recovery rate. The three kinds of active ingredients of ginkgolides, flavonol glycosides and biflavones contained in the Ginkgo biloba L. leaves can be measured at the same time. The defect that anexisting medicinal material quality control technology is not complete and scientific enough is made up. Quality of the Ginkgo biloba L. medicinal material can be accurately and comprehensively controlled.

The invention discloses a making method of separated and purified new bis-flavonoid from dragon's blood, which is characterized by the following: adopting dragon's blood as raw material; extracting through ligarine, acetone, acetic ester solvent sequently; separating and recrystallizing the acetic ester solvent extract through silica gel column or rapid silica gel column to chromatograph; obtaining the product named 8-(3-(4-hydroxy-2-methoxybenzene)-1-(4-hydroxy benzene) propyl )-2-(4-hydroxy phenyl )-7-methoxy -4H-benzopyranyl -4-ketone to inhibit alpha-glucosidase acitivty and reduce blood sugar.

The invention discloses application of ginkgo biflavonoids to preparation of a medicine for preventing and treating asthma. The ginkgo biflavonoids are at least one of 7-demethylated biflavones, ginkgetin, sciadopitysin, and isoginkgetin. The invention discloses a medicinal composition for preventing and treating the asthma, the composition comprises ginkgo biflavonoids, and further comprises at least one of radix glycyrrhizae, white mulberry root-bark and semen armeniacae amarum. The invention further discloses a pharmaceutical composition formed by mixing ginkgo biflavonoids and a pharmaceutically acceptable excipient, and the mass percent of ginkgo biflavonoids is greater than and equal to 2.5%. The ginkgo biflavonoids can inhibit the recruitment of inflammatory cells in lungs, reduce the expression of inflammatory factors in the lungs, and lower the expression of PI3K-related proteins in inflammation pathways, and are free of obvious toxic or side effects and safe and reliable after being taken for a long term.

The invention provides an anti-virus or/and anti-tumor drug and a preparation method thereof and an application of fenugreek biflavone glycosides for preparing anti-virus or/and anti-tumor drugs. The invention provides the application of the chemical component fenugreek biflavone glycosides in fenugreek seeds as drugs for the first time, and uses the fenugreek biflavone glycosides for preparing anti-virus drugs and anti-tumor drugs for the first time. In vivo and in vitro experiment research shows that fenugreek biflavone glycoside tablets or fenugreek biflavone glycoside capsules of the invention have obvious effect on resisting influenza viruses, and the anti-influenza drug effect of the fenugreek biflavone glycoside tablets or fenugreek biflavone glycoside capsules of the invention is superior to the anti-influenza drug effect of the existing Western medicine ribavirin tablets. The anti-HBV effect of the fenugreek biflavone glycoside tablets or fenugreek biflavone glycoside capsules is superior to the anti-HBV effect of kurarinone capsules, and the anti-HIV effect of the fenugreek biflavone glycoside tablets or fenugreek biflavone glycoside capsules is superior to the anti-HIV effect of zidovudine tablets. The fenugreek biflavone glycoside tablets or fenugreek biflavone glycoside capsules have obvious effect on resisting proliferation of liver cancer, gastric cancer, intestinal cancer, lung cancer and cervical cancer cells, and the anti-tumor effect of the fenugreek biflavone glycoside tablets or fenugreek biflavone glycoside capsules is superior to the anti-tumor effect of the existing chemical component preparation hydroxycamptothecine injection.

The present invention relates to an improved multi-step method for preparing an extract from ginkgo biloba having a reduced content of 4'-O-methyl pyridoxine and/or biflavones comparing with the original extractive. The invention further relates to an extract from ginkgo biloba having a reduced content of 4'-O-methyl pyridoxine and/or biflavones comparing with the original extractive, which is obtainable by the method according to the present invention, as well as to its use.

The invention relates to a pharmaceutical composition used for treating liver cancer. The pharmaceutical composition is prepared by taking peimine, curcumenol, hinokiflavone, and justicidin A as drug substances via mixing. Different dosage forms can be obtained via conventional pharmaceutical technology. The pharmaceutical composition possesses significant importance on preparation of medicines used for treating liver cancer.

The invention provides a biflavonoid compound. The compound has a structural formula shown in the description. The invention also provides a preparation method of the biflavonoid compound, and relatesto a pharmaceutical preparation containing the biflavonoid compound and an application of the biflavonoid compound in preparation of a medicine for treating hepatocellular carcinoma and a medicine for protecting liver. According to the compound provided by the invention, as a natural small-molecule compound, (5,7,8,4'-tetrahydroxyflavone)-3'-4-(5,7-dihydroxyflavone) has lower toxicity to normal cells and less side effects; in addition to a good function of protecting the liver, the biflavonoid compound also exhibits significant hepatocellular carcinoma-resistant effects, and the biflavonoid compound can provide a novel therapeutic medicine and therapeutic route for patients with the hepatocellular carcinoma, and has the advantages of a moderate effective dose and significant curative effects, small toxic and side effects; therefore, the biflavonoid compound has broad application prospects.

The invention discloses biflavone compounds. The biflavone compounds are compounds obtained by connecting any one compound as shown in a formula I, a formula II or a formula III with any one compoundas shown in the formula I, the formula II and the formula III in a 2'-2''' C-C connection mode, or pharmaceutically acceptable salts thereof, wherein the formula I, the formula II and the formula IIIare as described in the specification. According to the biflavone compounds provided by the invention, the compounds as shown in a formula VII, a formula VIII and a formula IX are natural biflavone compounds separated from hovenia dulcis thunb. The results of in-vitro anti-respiratory syncytial virus activity tests and anti-inflammatory activity tests show that the biflavone compounds as shown inthe formula VII, the formula VIII or the formula IX have remarkable antiviral activity and anti-inflammatory activity, and can be used for preparing drugs or food with antiviral and/or anti-inflammatory effects.

The invention relates to novel biflavone compound Yunjingsu as well as application and a preparation method thereof, and a biological activity experiment shows that the biflavone compound has GPR35 agonistic activity and can be used in medicines for preventing and treating diseases such as hypertension, asthma, pain, inflammation and tumors related to GPR35 receptors.

The invention discloses application of amentoflavone in preparation of a medicine for promoting wound healing. The amentoflavone is a flavonoid small molecule compound, and a brand new agonist is developed on the basis of a novel target point GPR39 for promoting wound healing. Experiments prove that the amentoflavone has obvious activity of promoting wound healing by exciting a GPR39 target spot, and can be used for research and development of medicines for promoting wound healing.

The compounds of the present invention comprise biflavonoid-sialic acid conjugates. Such biflavonoid-sialic acid conjugates show anti-influenza virus activities, not only in in vitro systems using cultured cells, but also in in vivo systems using mice. Thus, they are useful as preventive or therapeutic agents for influenza, and moreover, are useful in food and drink products for preventing or treating influenza.

The present invention relates to a cosmetic composition for improving skin wrinkles, which contains biflavonoid derivatives as an active ingredient. The cosmetic composition containing the biflavonoid derivatives of the present invention not only is safe for application on the skin without side-effects but also improves wrinkles caused by UV through procollagen production and MMP-I inhibition.

The invention discloses a method for detecting the content of various biflavones in a ginkgo leaf preparation. The method can detect amentoflavone, ginkgetin, isoginkgetin, bilobetin and sciadopitysinin the ginkgo leaf preparation simultaneously, an HPLC(Waters e2695) and Welch Bolimate C18 column is adopted for determination; a mobile phase is acetonitrile-water, and gradient elution is conducted, wherein the volume fraction of acetonitrile is 40%-80%, the flow velocity is 0.5 ml/min, the detection wavelength is 330 nm, and the column temperature is 30 DEG C; automatic sample introduction isconducted on standard substances and samples for test separately, and the content of five biflavones is calculated with an external standard method. Accordingly, by means of detection of the biflavone content, the quality control standard of the ginkgo leaf preparation is greatly improved; complex pretreatment is not needed, rapid detection can be achieved, the detection time is saved, and the detection cost is reduced.

The invention relates to a preparation method of two diflavonoid compounds with antitumor activity in sinopodophyllum emodi and application thereof. The preparation method of the two novel diflavonoidcompounds with the antitumor activity in the sinopodophyllum emodi, namely sinodiflavonoid A and sinodiflavonoid B, comprises the following steps: preparing a crude extract, preliminarily separatingthrough combination of silica gel column chromatography and gel column chromatography, and purifying by high performance liquid chromatography. The preparation method is stable, reliable and high in efficiency; by the preparation method, the two novel diflavonoid compounds with the antitumor activity, namely the sinodiflavonoid A and the sinodiflavonoid B, can be quickly separated from the sinopodophyllum emodi; in addition, results of an activity test show that the sinodiflavonoid A and the sinodiflavonoid B have cytotoxic activity for human cervical cancer cells (HeLa), and can be effectively applied to preparation of medicines for resisting cervical cancer, so that the medicinal value and novel application of the sinopodophyllum emodi are opened up, the commercial value of the sinopodophyllum emodi is increased, and the economic and social benefits are huge.

The invention discloses an application of bioflavonoids, and mainly provides an application of a compound shown in the formula (I) to preparation of a colitis treatment drug, wherein R1 is hydrogen orhydroxyl. When the compound acts on DSS-induced enteritis of a mouse, it can be found that the compound can relieve symptoms of colitis of the mouse, the compound has a good therapeutic effect on colitis and can be applied to preparation of the colitis treatment drug, and the application range of the drug is expanded.