99 results about "Sesquiterpene" patented technology

The application belongs to the technical field of microorganisms and particularly relates to a kind of Aspergillus ustus and application thereof.The Aspergillus ustus is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.40820.The Aspergillus ustus can be separated to obtain sesquiterpenes shown in formula I and formula II, and the sesquiterpenes have good bacteriostatic activity on Physarum polycephalum, and the bacteriostatic rates are 79.45% and 76.71% respectively at a final concentration of 100 μg / mL, which is equivalent to the control agent of bercyn in inhibiting mycelial growth.

This invention discloses a genetic transformation and gene editing method applicable to multiple sesquiterpene species, belonging to the field of plant genetic transformation and gene editing technology. The method uses the cotyledonary nodes of sterile seedlings of the sesquiterpene genus as explants, and introduces an optimized pScEF1α-Cas9 gene editing transformation vector using Agrobacterium infection. Regenerated plants are obtained through recovery culture, selection culture, and rooting culture. The vector contains four gene expression units: pScEF1α:Cas9, pGmU6:sgRNA, pGmUBI:DsRed2, and pCaMV35S:HygR. In vivo screening is further performed using the DsRed2 fluorescent reporter gene, and the gene-edited plants are identified by molecular detection. This method is applicable to common sesquiterpene, stem-nodled sesquiterpene, and spiny sesquiterpene, and has advantages such as strong species versatility, high transformation efficiency, short cycle, visualized screening, and high positive rate, providing efficient technical support for gene function research and genetic improvement of sesquiterpene species.

The application relates to application of a sesquiterpenoid compound in preparation of a medicine for preventing and / or treating pulmonary fibrosis. The sesquiterpenoid compound has obvious inhibiting collagen deposition effect on TGF-beta 1 induced human embryo lung fibroblasts, does not show cytotoxicity, has good anti-pulmonary fibrosis activity, and can be used for preparing the medicine for preventing and / or treating pulmonary fibrosis.

The present invention refers to a method for the preparation of a compound according to formula (45), its enantiomers, diastereomers, a racemic mixture, and the pharmaceutically acceptable salts thereof. Furthermore the invention refers to a compound of formula (45) prepared by said method for use in treatment of neurodegenerative disorders and to pharmaceutical compositions.

The application belongs to the field of enzyme genetic engineering, and particularly relates to application of a sesquiterpene synthase and a mutant thereof in synthesis of enantiomeric-drimenol compounds, wherein the sesquiterpene synthase is a sesquiterpene synthase from Chenopodium quinoa Willd., and includes CqDMDS1 or CqDMDS2; the mutant of the sesquiterpene synthase is a mutant CqDMDS1 in which an amino acid at position 443 of CqDMDS1 is mutated F443A / G The application realizes heterologous expression of the sesquiterpene synthase and the mutant thereof from Chenopodium quinoa Willd. in an Escherichia coli and a N. benthamiana expression system, catalyzes synthesis of (-)‑8,11-driman-diol or (+)‑drimenol. It is found through activity test that the sesquiterpene synthase from Chenopodium quinoa Willd. has a significant inhibitory effect on plant pathogenic fungi Phytophthora nicotianae, Phytophthora sojae and Fusarium graminearum. The sesquiterpene synthase and the mutant thereof and the biological preparation of the catalytic product have important significance for development of biological pesticides.

The invention belongs to the technical field of medicinal plant cultivation, and particularly provides a method for increasing the content of sesquiterpene glycoside in dendrobium nobile. The method comprises the step of applying a magnesium ion-containing solution with effective concentration at the growth stage of dendrobium nobile. Preferably, the magnesium ion-containing solution is a magnesium chloride solution, the application concentration is 10-500 mg / L, the magnesium ion-containing solution is applied in a foliage spraying or root irrigation mode, and the key period of application is the rapid growth period or the effective component accumulation period of the dendrobium nobile. The promotion effect of exogenous magnesium ions on biosynthesis of the sesquiterpene glycoside of the dendrobium nobile is disclosed for the first time, and the content of sesquiterpene glycoside components in the dendrobium nobile medicinal material can be remarkably increased under the condition that a basic cultivation mode is not changed, so that the medicinal potential and market value of immunoregulation, anti-inflammation and the like of the dendrobium nobile medicinal material are enhanced. The method is simple, efficient, low in cost, free of pollution and suitable for industrial application.

The invention relates to the technical field of agriculture, and provides germacane type sesquiterpenoids in tithonia diversifolia as well as a preparation method and application thereof.The preparation method comprises the steps that stem and leaf parts of tithonia diversifolia are subjected to ethanol cold-soaking extraction, and an extracting solution is subjected to vacuum concentration to obtain a total extract; suspending the total extract with water, sequentially extracting with petroleum ether and ethyl acetate, and concentrating the extract liquor under reduced pressure to obtain each extraction layer; carrying out silica gel column chromatography separation on the ethyl acetate extraction layer, and combining to obtain six parts A-F; carrying out MCI column chromatography separation on the part D, and combining to obtain thirteen parts D1-D13; carrying out silica gel column chromatography separation on the D9 part, and combining to obtain fifteen parts D91-D915; and separating the D915 part by adopting HPLC (High Performance Liquid Chromatography) to prepare two germacane type sesquiterpenoids. The preparation method disclosed by the invention is simple and good in reproducibility, and the prepared germacane type sesquiterpenoids are high in purity and have specific herbicidal activity on foreign invasive plants such as ambrosia trifida and ambrosia esculenta.

The application belongs to the technical field of biology, and discloses application of CmPHL1 transcription factor in inhibition of citrus leaf oil cell formation and regulation of volatile substances. In view of the problem of insufficient research on existing citrus oil cell formation and essential oil synthesis, a pGBI-CmPHL1 overexpression vector is constructed, and positive strains are obtained by means of Agrobacterium-mediated transformation of citrus. Experiments prove that overexpression of CmPHL1 can significantly reduce the number of oil cells and the content of monoterpenes, sesquiterpenes and other volatile substances. CmPHL1 targets the molecular regulation network of oil cell development, accurately regulates and is heritable, provides a new gene target for citrus quality improvement and breeding, is in line with the direction of green agriculture, and has important economic and ecological benefits.

Sesterterpenoid compounds and an extract L01 of Leucosceptrum canum, and use thereof in treatment of psoriasis are provided, belonging to the technical field of natural medicinal chemistry. The sesterterpenoid compound of the Leucosceptrum canum has a structure shown in any one of Formulas 9 to 12, which shows a highly rare backbone type, exhibits anti-inflammatory and immunosuppressive activities, and can be used in preparation of an anti-inflammatory drug and an immunosuppressive drug. A Leucosceptrum canum extract L01 having compounds 1 to 12 as characteristic ingredients, and use thereof in preparation of an anti-inflammatory drug and an immunosuppressive drug are further provided.

This invention provides a method for the separation, purification, and aroma quality control of biosynthesized patchouli essential oil, belonging to the field of daily chemical product purification technology. It solves the technical problems of low separation efficiency, insufficient purity, easy destruction of heat-sensitive components, and low aroma matching in existing essential oils. This method uses metabolically engineered brewer's yeast fermentation broth as raw material, sequentially undergoing pretreatment, multi-stage countercurrent extraction, three-stage high-vacuum distillation, column chromatography purification, activated carbon decolorization and deodorization, aroma component recombination, and product stabilization to obtain high-purity patchouli essential oil with high aroma matching. This invention is adapted to the three-stage distillation process of biosynthetic systems, achieving efficient separation of solvents, sesquiterpenes, and patchouli alcohol, resulting in high purity; the product aroma has a high matching degree with natural Indonesian essential oils; the process is mild throughout, can be stably scaled up from small-scale to ton-scale, and is low-cost. This invention provides a complete, efficient, and green technical solution for the industrialization of natural fragrances derived from synthetic biology.

The application discloses a pterosin sesquiterpenoid compound and a preparation method and application thereof, belongs to the technical field of biological medicines, aims at the problems of a research blank of pterosin sesquiterpenoid compounds in Anemone raddeana and a lack of structure-confirmed anti-inflammatory active monomers, and provides a pterosin sesquiterpenoid compound with a structural formula as shown in the following.The compound preparation needs to first crush dried whole grass of Anemone raddeana, extract by heating and refluxing with an organic solution, concentrate under reduced pressure to obtain total extract; then, the extract is extracted by petroleum ether, ethyl acetate and n-butanol step by step, the ethyl acetate part extract is enriched, and the high-purity product is prepared through multi-step purification of a silica gel column, a C-18 reverse phase column, a gel column and semi-preparative liquid chromatography.In addition, the compound can dose-dependently inhibit the NO release of LPS-induced macrophages, and can be used for preparing anti-inflammatory drugs for treating or preventing inflammatory diseases related to excessive production of nitric oxide, fills the research blank, and provides a high-quality candidate component for anti-inflammatory drug research and development.

This invention discloses a eucalyptane-type sesquiterpene dimer compound, its preparation method, and its applications, belonging to the field of pharmaceutical preparation technology. The six eucalyptane-type sesquiterpene dimers (compounds 1-6) disclosed in this invention are novel compounds with defined stereostructures and optically pure composition, exhibiting varying degrees of cytotoxic activity against HepG2, Hep3B, and Huh7 cells. Using sorafenib as a positive control, compounds 1, 5, and 6 showed stronger cytotoxic activity against HepG2 cells than the positive control sorafenib, compound 5 exhibited the strongest cytotoxic activity against Hep3B cells, and compounds 1, 4, 5, and 6 showed stronger cytotoxic activity against Huh7 cells than the positive control sorafenib. Therefore, compounds 1-6 disclosed in this invention have the potential to be developed into anti-hepatocellular carcinoma drugs, showing promising application prospects, and also provide important theoretical support for the further development of Atractylodes macrocephala medicinal plant resources.

This invention belongs to the field of pharmaceutical technology, specifically relating to the use of a sesquiterpene compound and / or its pharmaceutically acceptable salt in the preparation of medicaments for treating or preventing infectious diseases caused by Staphylococcus aureus, particularly in the preparation of medicaments for treating or preventing infectious diseases caused by drug-sensitive or drug-resistant Staphylococcus aureus. The structure of the sesquiterpene compound of this invention is shown in Formula I: [Formula I]. The sesquiterpene compound used in this invention is derived from *Chrysanthemum indicum*, which is widely available and has low production costs. This invention is the first to discover that the sesquiterpene compound shown in Formula I exhibits very good inhibitory activity against Staphylococcus aureus, including very good inhibitory activity against drug-sensitive, drug-neutral, or drug-resistant Staphylococcus aureus.

This invention discloses a heterozygous sesquiterpene tricyclic epoxy compound derived from the strain *Arthrum oligosporum*, its preparation method, and its applications, belonging to the field of natural product chemistry. This invention provides a heterozygous sesquiterpene tricyclic epoxy compound derived from *Arthrum oligosporum* (… A.oligospora ) AOL_ s00210g57 Anthrobotrisin D, a novel heterozygous sesquiterpene tricyclic epoxy compound from a gene knockout strain, has been shown in vitro to possess excellent anti-inflammatory activity, significantly inhibiting lipopolysaccharide-induced inflammatory responses in mouse microglia. It exhibits a clear dose-dependent inhibitory trend at multiple levels, including NO release, protein and mRNA expression of inflammatory factors (TNF-α, IL-1β, IL-6), and iNOS protein and mRNA expression. Furthermore, at a concentration of 33 μM, its anti-inflammatory effect is comparable to that of the clinically proven positive drug dexamethasone, and in some aspects, it is even superior. This invention not only provides a new lead compound for the development of anti-inflammatory drugs but also opens up new directions for developing new drugs from the unique microbial resource of nematode-preying fungi.

The invention provides a method for determining the content of two sesquiterpenoids in a turmeric extract, and belongs to the technical field of medicine detection.The quality detection method comprises the steps that the turmeric extract is taken to be prepared into a test solution, then acetonitrile (A) and 0.08-0.12 wt% phosphoric acid aqueous solution (B) are subjected to isocratic elution with the ratio of 65-75: 25-35, then an external standard one-point method or a standard curve method is used for determining the content of the two sesquiterpenoids in the turmeric extract, and the content of the two sesquiterpenoids in the turmeric extract is determined. And calculating the content of aryl turmerone and beta-turmerone in the turmeric extract. The method for determining the content of the two sesquiterpene compounds in the turmeric extract is innovatively established, accurate quantitative analysis of the target sesquiterpene component is realized for the first time by optimizing chromatographic separation conditions and detection parameters, the detection blank of an existing standard system is filled, and the method has a wide application prospect. And a key technical support is provided for constructing a turmeric extract multi-dimensional quality control system.

This invention discloses a mango terpene synthase gene TPS4 and its applications, the nucleotide sequence of which is shown in SEQ ID NO:1. The amino acid sequence of the protein it encodes is shown in SEQ ID NO:2. The invention also discloses a recombinant expression vector containing the mango terpene synthase gene TPS4, a recombinant engineered strain, and the recombinant mango terpene synthase TPS4; and the application of the mango terpene synthase gene TPS4, its encoded protein, the recombinant expression vector, the recombinant engineered strain, or the recombinant TPS4 in regulating the synthesis of terpenoid compounds from geraniol diphosphate (GPP), neroli diphosphate (NPP), and farnesyl diphosphate (FPP). This invention is the first to clone and verify a terpene synthase gene TPS4 from the Sacred Heart mango genome, capable of simultaneously catalyzing the synthesis of monoterpenes and sesquiterpenes from three substrates: GPP, NPP, and FPP. It has broad application prospects and significant economic value.

The present invention provides novel water-soluble compositions comprising sesquiterpene coumarin enriched fractions containing different ratios of sesquiterpene coumarin derived from Ferula species for the treatment of Parkinson's disease via inhibition of MAOenzyme (MAO-A and MAO-B). The invention also provides a method of preparation of ethyl-acetate extract and sesquiterpene coumarin enriched fractions from asafoetida (Hing) that is oleo-gum-resin of Ferula assafoetida using silica gel column chromatography wherein the mobile phase consists of differing compositions of hexane, toluene, chloroform,ethyl-acetate, and methanol. The invention provides the method to prepare the formulation using a multistep process comprising of preparing sesquiterpene coumarin enriched fraction followed by preparing water-soluble formulation. The final composition improves the water solubility, imparts long-term stability, exerts MAO inhibitory activity, and improves pharmaceutical compliance.

The invention discloses judgment and optimization of key amino acid sites for controlling sesquiterpene synthesis, and determines amino acid residues lysine or arginine or glutamine related to sesquiterpene synthesis for point mutation and combined mutation of various plant sesquiterpene synthase. Lysine or arginine or glutamine is located in a cavity among 5-10 amino acids in front of the first long alpha helix at the N end, alpha helixes (also the longest alpha helix in the protein) communicating the N end and the C end of the protein and the last 1-7 amino acids at the C end at the site. The method for determining the key amino acid can be used for efficiently screening the sesquiterpene synthase with activity, and the optimization of the amino acid of the key residue can be used for guiding the transformation of the high-yield sesquiterpene synthase for industrial production. The invention has important application value and economic benefit in the fields of biological synthase identification of sesquiterpenoids, biosynthesis of drugs, fermentation production of spices, synthesis of industrial raw materials and the like.

The application discloses a preparation method of sesquiterpene derivatives. The method comprises the following steps: constructing a water phase / organic phase two-phase system by using GFPP buffer solution, SvSS enzyme, an incubation buffer of beta-cyclodextrin and an organic solvent, reacting at 25-35 DEG C for 1-24 hours, and obtaining the sesquiterpene derivatives through post-treatment after the reaction is completed. The preparation method of the sesquiterpene derivatives is suitable for scale-up application, and provides a reliable technical basis for further research and development of sesterviolene A.

The invention belongs to the technical field of natural pharmaceutical chemistry and medicine, and relates to a sesquiterpene compound and a separation method thereof, and application of the sesquiterpene compound in blood sugar reduction. According to the 14-acetylated 1-indanone type sesquiterpene glucoside compound disclosed by the invention, a new 14-acetylated 1-indanone type sesquiterpene glucoside compound, namely 14-acetyl-(2S, 3S)-Pterosine C-3-O-D glucoside, is separated and identified from pteris multifida poir for the first time. According to the compound, a new chemical entity is added for a natural product library, and valuable candidate molecules are provided for drug research and development. The sesquiterpene compound disclosed by the invention shows excellent alpha-glucosidase inhibitory activity; an in-vitro enzyme activity experiment shows that the sesquiterpene compound has a remarkable inhibition effect on alpha-glucosidase from yeast (the inhibition rate reaches 52.1% under the concentration of 10 mg / mL).

Methods and systems for extracting additional beneficial lipid-soluble heavy compounds from plant material in an environmentally sustainable manner. A method includes preparing a solution that includes a solvent comprising one or more of a monoterpene, a monoterpenoid, a sesquiterpene, or a sesquiterpenoid. The solution further includes a solute comprising a plant material. The method includes extracting one or more compounds from the plant material using the solvent comprising the one or more of the monoterpene, the monoterpenoid, the sesquiterpene, or the sesquiterpenoid.

The application relates to the technical field of plant chemistry and active ingredient heterocyclic compounds of traditional Chinese medicine, and discloses a C17 sesquiterpene alkaloid, a preparation method and application, wherein the C17 sesquiterpene alkaloid and a pharmaceutically acceptable salt thereof are shown as formula I; the C17 sesquiterpene alkaloid of formula I is separated from an ethanol extract n-butanol extraction phase of Dendrobium huoshanense stems through three-step operations of silica gel column chromatography, acid solution and base precipitation and recrystallization; the structure of the compound is determined through nuclear magnetic resonance, high-resolution mass spectrometry and single crystal diffraction analysis; experiments show that the C17 sesquiterpene alkaloid can effectively inhibit the survival and proliferation of A549 lung cancer cells, has good anti-lung cancer activity, and can be used for preparing anti-lung cancer drugs.

This invention discloses a green extraction method for high-purity vetiver essential oil and its application, belonging to the field of plant fragrance extraction technology. The invention first pre-treats the vetiver roots by drying and pulverizing them, then uses a two-stage supercritical carbon dioxide extraction process for fractional extraction. The crude essential oil is obtained through a two-stage separator, and then undergoes solvent removal, primary molecular distillation fractionation, silica gel gradient adsorption enrichment, and secondary molecular distillation purification to finally obtain high-purity vetiver essential oil. This invention, through differentiated supercritical extraction combined with the synergistic effect of multiple refining processes, can pre-remove non-polar impurities from the raw materials and directionally enrich the effective components of sesquiterpenes in vetiver, effectively improving the purity and component stability of the essential oil. The overall preparation process is green and pollution-free, with low solvent residue, standardized operation procedures, and is suitable for large-scale industrial production.

The invention relates to a spiro sesquiterpene compound as well as a preparation method and application thereof. The spiro sesquiterpene compound comprises a compound with a structure as shown in a formula (1). The compound provided by the invention is extracted from dry roots of a cultivated plant eggplant, can be used for preparing a pesticide, is safe to human and livestock, has remarkable activity on agricultural, warehousing and sanitary pests, and has a wide insecticidal spectrum, and the pests are not easy to generate drug resistance after being used. Formula (1)

The application discloses a sesquiterpene dimer and a preparation method and application thereof, and belongs to the technical field of natural compound extraction. According to the preparation method, dried sesquiterpene dimers are extracted by refluxing with 95% ethanol, and then petroleum ether and ethyl acetate are used for extraction to obtain an extract, which can be obtained by column chromatography and semi-preparative high performance liquid chromatography, so that the method is simple, time and labor are saved, and the yield is higher. The obtained sesquiterpene dimers have high anti-neuritis activity, and have important application prospects for developing new drugs for treating senile dementia.

The application belongs to the field of antibacterial activity research, and discloses a preparation method of guaiane sesquiterpenes and application thereof in resisting plant pathogenic fungi. Firstly, leaf blades of Artemisia stolonifera are dried, extracted, concentrated, and then a crude fraction is obtained through extraction, and then the crude fraction is further purified through normal silica gel, dextran gel and reversed-phase macroporous resin column chromatography, and finally three guaiane sesquiterpenes are obtained through liquid phase preparation. The compounds can effectively inhibit the growth of Sclerotinia sclerotiorum, Cochliobolus sativus and Verticillium dahliae, and the minimum inhibitory concentration (MIC) of moxartenolide is 6.25, 12.5 and 12.5 μg / mL respectively, the minimum inhibitory concentration (MIC) of artemdubolide C is 12.5, 12.5 and 12.5 μg / mL respectively, and the minimum inhibitory concentration (MIC) of artemvulactone F is 12.5, 12.5 and 12.5 μg / mL respectively, and the compounds have great application potential in resisting plant pathogenic fungi.

The application discloses a sesquiterpene derivative, a preparation method and application thereof. The application provides a novel sesquiterpene derivative which is obtained by modifying atractylodin III. Activity research shows that the sesquiterpene derivative has stronger inhibiting effect on IgE or histamine secretion than atractylodin III. It is known to those skilled in the art that allergy is mediated by IgE, and histamine secretion under the stimulation of IgE is an important cause of various allergic symptoms. Therefore, the sesquiterpene derivative provided by the application has the prospect of being developed into a medicine for preventing and / or treating diseases which are prevented and / or treated by inhibiting IgE or histamine secretion, such as allergic diseases like food allergy.

The invention discloses an antiproliferative synergistic antifungal drug composition based on a notopterygium incisum source, and relates to the technical field of drug preparation, the antiproliferative synergistic antifungal drug composition comprises a first active component and a second active component, the first active component is an anti-proliferative active component and is selected from at least one of the following two compounds: class A: giant column alkane sesquiterpenoids; the class B is a cycloartane type triterpenoid compound; the second active component is a synergistic antifungal active component and is composed of an active component A and an active component B; the pharmaceutical composition takes a specific active component from notopterygium incisum as a core, and can accurately induce apoptosis of target cells, efficiently inhibit proliferation of the target cells, remarkably enhance the antibacterial activity of the antifungal drug and greatly reduce the clinical dosage and toxic risk of the antifungal drug; the natural active components are excellent in biocompatibility and high in medication safety, and can synchronously realize dual effects of disease treatment and infection protection.

The application discloses a sesquiterpenoid compound and a preparation method and application thereof, and belongs to the field of medicines.The structural formula of the application is The separation and preparation method of the compound of the application is that the ethyl acetate part of the calyx of kadsura coccinea is divided into sections by a normal phase silica gel column, elution is carried out with CH2Cl2:MeOH (50:1-0:1), the obtained part is further eluted by normal phase CH2Cl2-MeOH (40:1-0:1) and reversed phase C18 with MeOH:H2O (30:1, 40:1, 50:1, 60:1, 80:1), and then separation and purification are carried out through gel and HPLC.The compound of the application can be used for preparing antioxidant medicines or as a lead compound for development of antioxidant medicines.

The invention discloses a hybrid sesquiterpene ternary epoxy compound derived from arthrobotrys oligospora strain as well as a preparation method and application thereof, and belongs to the technical field of natural product chemistry. The invention provides a novel hybrid sesquiterpene ternary epoxy compound anthrobotrisin D which is derived from an arthrobotrys oligospora AOLs00210g57 gene knockout strain, and an in-vitro experiment proves that the compound has excellent anti-inflammatory activity and has a remarkable inhibition effect on a lipopolysaccharide induced mouse microglial cell inflammatory reaction. The compound shows a clear dose-dependent inhibition trend in a plurality of levels such as NO release, protein and mRNA expression of inflammatory factors (TNF-alpha, IL-1beta and IL-6), protein and mRNA expression of iNOS and the like, and has an anti-inflammatory effect equivalent to that of a clinical positive drug dexamethasone when the concentration is 33 mu M, and a part of indexes even more excellent. The invention not only provides a new lead compound for research and development of inflammation treatment drugs, but also opens up a new direction for developing new drugs from a unique microbial resource of nematode-trapping fungi.