322 results about "Sesquiterpene" patented technology

The invention relates to the field of natural products, in particular to a dimer compound of guaiane type sesquiterpenes and uric acid as well as an extraction and separation method and application of the dimer compound. According to the invention, a new dimer compound of guaiane type sesquiterpene and uric acid is extracted and separated from carpesium abrotanoides in Guizhou, and it is found that the dimer compound has relatively strong anti-tumor activity. The compound has strong inhibitory activity on breast cancer, prostate cancer, nasopharynx cancer, liver cancer, lung cancer, leukemia, pancreatic cancer, glioma, osteosarcoma, skin cancer, cervical cancer, ovarian cancer, kidney cancer and esophageal cancer, and provides an optional scheme for broad-spectrum anti-cancer drugs. Moreover, in human prostate cancer cells PC3, human cervical cancer cells Hela, human liver cancer cells HepG2 and human glioma cells U87 which are resistant to paclitaxel, the compound also shows strong anti-cancer activity, which indicates that the compound has the potential of treating patients resistant to paclitaxel.

The invention belongs to the technical field of medicines, and particularly relates to application of sesquiterpenoids and / or pharmaceutically acceptable salts thereof in preparation of medicines for treating or preventing infectious diseases caused by staphylococcus aureus. The invention relates to the field of medicines, in particular to application in preparation of medicines for treating or preventing infectious diseases caused by drug-sensitive staphylococcus aureus or drug-resistant staphylococcus aureus. The structure of the sesquiterpenoids is shown as a formula I in the specification. The sesquiterpenoids applied in the invention are derived from rattan chrysanthemum, the source is wide, and the production cost is low. The sesquiterpenoids shown in the formula I have a very good activity inhibition effect on staphylococcus aureus for the first time, and the sesquiterpenoids have a very good activity inhibition effect on staphylococcus aureus with drug sensitivity, drug medium or drug resistance.

The invention provides a sesquiterpene dimer in artemisia selengensis, a pharmaceutical composition of the sesquiterpene dimer and a preparation method and application of the sesquiterpene dimer, and belongs to the technical field of medicines. According to the present invention, the eight new sesquiterpene dimers represented by the structural formula of the present invention, artemselenoids A-H (1-8) have significant inhibitory activity on three liver cancer cells (HepG2, Huh7 and SK-Hep-1), can form the pharmaceutical composition with the pharmaceutically acceptable carrier, and can be used for preparing the anti-liver cancer drug.

The invention discloses a phoebe zhennan PzGGPS12 gene and application of the phoebe zhennan PzGGPS12 gene to improvement of yield and drought tolerance of plant terpenoids, and belongs to the field of genetic engineering, a key enzyme gene PzGGPS12 for catalyzing biosynthesis of the terpenoids in phoebe zhennan wood is screened and analyzed, and a full-length CDS nucleic acid sequence of the PzGGPS12 is obtained. Transgenic experiments prove that overexpression of the gene improves the relative content of tobacco monoterpenes and triterpenes. Short-term drought experiments prove that overexpression of the gene can promote biosynthesis of sesquiterpenes, diterpenes and triterpenes so as to enhance drought tolerance of transgenic tobacco. Therefore, the gene can be introduced into a plant as a target gene, the yield of terpenoids in the plant is increased, and the drought tolerance of the plant is enhanced so as to improve the variety of the plant.

The invention discloses a marine streptomyces strain SHEa3, a compound derived from the marine streptomyces strain SHEa3 and application of the marine streptomyces strain SHEa3, the classification name of the marine streptomyces strain SHEa3 is Streptomyces sp, the preservation number of the marine streptomyces strain SHEa3 is GDMCC No: 66667, and the preservation date of the marine streptomyces strain SHEa3 is July 14, 2025. The invention also discloses a sesquiterpenoids compound streptaviol A derived from marine streptomyces and application of the sesquiterpenoids compound streptaviol A in preparation of drugs for preventing and treating vibrio diseases of mariculture animals. The invention further discloses application of the sesquiterpenoids, namely the sea threitol C, derived from the marine streptomyces in preparation of drugs for preventing and treating vibrio diseases of mariculture animals. The marine streptomyces source compound has efficient anti-vibrio activity, and the marine streptomyces source compound streptaviol A and the helithreitol C are used for preparing the medicine for preventing and treating vibrio diseases of mariculture animals, so that the side effect is low, and the activity is excellent.

The invention discloses a sesterterpenoid compound, a preparation method thereof and application of the sesterterpenoid compound in preparation of a medicine for treating psoriasis, and belongs to the technical field of biological medicine. Comprising a compound 1-4, and the structural formula of the compound 1-4 is as follows: # imgabs0. A new molecular template is provided for developing a new psoriasis treatment medicine, and a new thought is provided for further developing a novel treatment strategy with an immunomodulatory effect.

The present invention relates to processes for the oxidation of sesquiterpenes comprising fused 5- and 7-membered rings using mutant Cytochrome P450 family 102A sub-family member (CYP102A) enzymes. The invention also relates to mutant CYP102A enzymes, polynucleotides encoding the enzymes, cells expressing the enzymes, transgenic animals or plants comprising the enzymes, and compounds produced by the processes.

The invention discloses a saccharomyces cerevisiae recombinant strain based on a uracil metabolism gene URA3 as well as a construction method and application of the saccharomyces cerevisiae recombinant strain. The construction method comprises the following steps: taking saccharomyces cerevisiae rich in farnesyl pyrophosphate as an original strain, introducing a uracil metabolism gene URA3, synchronously overexpressing a ribosome biosynthetic gene UTP10 and an iron metabolism gene FIT3, and utilizing the uracil metabolism gene URA3 to express up-regulation correlation with the ribosome biosynthetic gene UTP10 and the iron metabolism gene FIT3, so as to obtain the farnesyl pyrophosphate-rich saccharomyces cerevisiae. The metabolic flux of the terpenoids is enhanced, and the saccharomyces cerevisiae recombinant strain for efficiently producing the terpenoids is constructed. According to the method, a metabolic network of yeast is systematically optimized, and combined regulation and control on precursor flux distribution, protein translation capability and metal ion steady state are realized, so that the microbial synthesis efficiency of sesquiterpenoids such as patchouli alcohol and beta-elemene is remarkably improved.

The invention discloses a directed evolution strategy of type II sesquiterpene cyclase (SsDMS) derived from Streptomyces shedoensis based on alanine scanning, and an application of the type II sesquiterpene cyclase (SsDMS) derived from Streptomyces shedoensis based on alanine scanning. The invention relates to a plurality of SsDMS key mutant sites capable of catalytically generating a novel drive type sesquiterpenoid compound, which comprise substitution of sites 208, 248, 249, 497 and 505, and the SsDMS key mutant sites comprise substitution of sites 208, 248, 249, 497 and 505. The invention discloses an efficient escherichia coli cell factory applied to heterologous expression of an SsDMS mutant and efficient production of a novel drive type sesquiterpene compound, and further obtains the drive type sesquiterpene compound with a novel structure. According to the present invention, the efficient production of the drive type sesquiterpene compound by using the SsDMS and the mutant thereof is achieved, the modification strategy has wide applicability, the important enzymology resource is provided for the biosynthesis of the novel terpene compound, and the more choices are provided for the discovery of the drug lead compound.

The invention discloses an extraction method and application of an aza-sesquiterpenoid compound which is derived from marine fungi and has anti-prostatic cancer activity. The structure of the aza-sesquiterpenoid compound is shown as a formula I-3 or pharmaceutical salt of the aza-sesquiterpenoid compound: # imgabs0 #, wherein the compound 1 is pyripyropene E, the compound 2 is pyripyropene O, and the compound 3 is 11-deacetylated pyripyropene A (11-deacetylated PPPA). The invention further discloses a preparation method of the aza-sesquiterpenoid compound with the anti-prostatic cancer activity and application of the aza-sesquiterpenoid compound with the anti-prostatic cancer activity and application of the aza-sesquiterpenoid compound with the anti-prostatic cancer activity. Molecular docking analysis shows that the compound shows anti-prostatic cancer activity, further experiments find that the compound pyripyroene O (compound 2) has remarkable cytotoxic activity on prostatic cancer cells, an alternative compound is provided for developing new prostatic cancer treatment, and the compound 2 has important significance on development of marine drug resources in China.

The invention relates to the technical field of bioengineering, in particular to a saccharomyces cerevisiae engineering strain for producing caryophyllene and application of the saccharomyces cerevisiae engineering strain. The invention provides a saccharomyces cerevisiae engineering strain which has enhanced expression and / or activity of mevalonic acid pathway enzyme and one or more modifications selected from the following (1)-(2): (1) weakened expression and / or activity of sterol synthesis pathway enzyme; (2) attenuated expression and / or activity of fatty acid synthetic pathway enzymes; compared with an original strain, the saccharomyces cerevisiae engineering strain has the advantage that the sesquiterpene yield is increased. Compared with an original strain, the saccharomyces cerevisiae engineering strain has the advantages that the production capacity of the sesquiterpenes such as the caryophyllene is obviously improved, the sesquiterpenes such as the caryophyllene can be efficiently synthesized, and the saccharomyces cerevisiae engineering strain has better application value.

The invention discloses a cadinane type sesquiterpene as well as a preparation method and application thereof. The cadinane type sesquiterpene is separated from cinnamon bark, and has at least one of structural formulas shown as a formula (I), a formula (II), a formula (III) and a formula (IV). Liver protection activity evaluation is carried out by applying an alcohol-induced hepatocyte injury model, and it is found that the cadinane type sesquiterpene can effectively relieve alcohol-induced AML-12 hepatocyte injury; the dumarane sesquiterpenes can inhibit the alcoholic liver disease and relieve fat accumulation, so that the dumarane sesquiterpenes have the anti-alcoholic liver disease activity, can be applied to preparation of the anti-alcoholic liver disease drugs, and have good research and development prospects; belongs to the field of natural medicinal chemistry.

The invention provides a function and application of a transcription factor NAC42. The invention discloses a novel gene NAC42 which is closely related to regulation and control of phytoalexin, volatile monoterpenes and sesquiterpenes of plants (so as to regulate and control insect resistance). The invention provides a new way for improving the insect resistance of plants.

The invention relates to application of an Artemisia rupestris extract in preparation of medicines for preventing and treating lung diseases. According to the application of the artemisia rupestris extract in preparation of the medicine for preventing and treating the lung diseases, the artemisia rupestris extract is prepared by taking artemisia rupestris as a raw material, and the crude drug concentration is 20-110 mg / mL. The artemisia rupestris extract contains chemical components such as sesquiterpenoids, flavonoids, organic acids and phenylpropanoids. The medicine is a veterinary medicine. The lung diseases comprise allergic pneumonia, pulmonary fibrosis or diseases caused by injury of other lung tissues. The dosage form of the medicine for preventing and treating the lung diseases is a pharmaceutically acceptable dosage form. The pharmaceutically acceptable dosage form is oral liquid, powder, tablets, granules, capsules, pills, syrup, suspension or emulsion. The application defines the prevention and treatment effect of the Artemisia rupestris extract on lung diseases. During application, the artemisia rupestris extract is small in dosage and high in curative effect, and the weight of livestock and poultry can be greatly reduced due to the fact that lung tissue is damaged.

The invention discloses a biosynthetic gene cluster of a novel skeleton sesquiterpenoid (sestercyclic acid) and an application of the biosynthetic gene cluster of the novel skeleton sestercyclic acid (sestercyclic acid). The sesterterpene tercyclic acid biosynthetic gene cluster at least comprises three genes, namely, terA, terB and terC, and the nucleotide sequences of the sesterterpene tercyclic acid biosynthetic gene cluster are as shown in SEQ ID NO.1-3. The gene cluster of the terrecyclic acid compound disclosed by the invention is found for the first time, and each gene function is also confirmed for the first time. Protease TerA catalyzes farnesyl pyrophosphate (FPP) to be cyclized into a new skeleton sesquiterpene 25, then P450 protease TerB is oxidized into a compound 32, and the compound 32 is dehydrogenated through protease TerC to form a final product compound 2. According to the present invention, the effective drug source approach is provided for the development of the terrecyclic acid antitumor compound.

The application belongs to the technical field of microorganisms and particularly relates to a kind of Aspergillus ustus and application thereof.The Aspergillus ustus is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.40820.The Aspergillus ustus can be separated to obtain sesquiterpenes shown in formula I and formula II, and the sesquiterpenes have good bacteriostatic activity on Physarum polycephalum, and the bacteriostatic rates are 79.45% and 76.71% respectively at a final concentration of 100 μg / mL, which is equivalent to the control agent of bercyn in inhibiting mycelial growth.

This invention discloses a genetic transformation and gene editing method applicable to multiple sesquiterpene species, belonging to the field of plant genetic transformation and gene editing technology. The method uses the cotyledonary nodes of sterile seedlings of the sesquiterpene genus as explants, and introduces an optimized pScEF1α-Cas9 gene editing transformation vector using Agrobacterium infection. Regenerated plants are obtained through recovery culture, selection culture, and rooting culture. The vector contains four gene expression units: pScEF1α:Cas9, pGmU6:sgRNA, pGmUBI:DsRed2, and pCaMV35S:HygR. In vivo screening is further performed using the DsRed2 fluorescent reporter gene, and the gene-edited plants are identified by molecular detection. This method is applicable to common sesquiterpene, stem-nodled sesquiterpene, and spiny sesquiterpene, and has advantages such as strong species versatility, high transformation efficiency, short cycle, visualized screening, and high positive rate, providing efficient technical support for gene function research and genetic improvement of sesquiterpene species.

The invention discloses curcuma wenyujin sesquiterpene synthase CwTPS8 as well as a coding gene and application thereof, and belongs to the technical field of plant genetic engineering, the amino acid sequence of the curcuma wenyujin sesquiterpene synthase CwTPS8 is shown as SEQ ID NO.1, the nucleotide sequence of the gene for coding the curcuma wenyujin sesquiterpene synthase CwTPS8 is shown as SEQ ID NO.2, and the nucleotide sequence of the gene for coding the curcuma wenyujin sesquiterpene synthase CwTPS8 is shown as SEQ ID NO.1. The curcuma wenyujin sesquiterpene synthase CwTPS8 can be used for preparing beta-caryophyllene, alpha-humulene and other curcuma wenyujin sesquiterpenoids by taking farnesyl pyrophosphate FPP as a substrate, and the technology can be used for subsequently producing the sesquiterpenoids through a microbial cell factory. Meanwhile, a foundation is laid for heterologous synthesis and further development and utilization of sesquiterpenoids such as beta-caryophyllene and alpha-humulene.

The application relates to application of a sesquiterpenoid compound in preparation of a medicine for preventing and / or treating pulmonary fibrosis. The sesquiterpenoid compound has obvious inhibiting collagen deposition effect on TGF-beta 1 induced human embryo lung fibroblasts, does not show cytotoxicity, has good anti-pulmonary fibrosis activity, and can be used for preparing the medicine for preventing and / or treating pulmonary fibrosis.

The invention relates to a recombinant genetically engineered bacterium for synthesizing terpenoids. The recombinant genetically engineered bacterium is saccharomyces cerevisiae in which a gal80 gene, an lpp1 gene and a dpp1 gene are knocked out and a heterologous MVA pathway gene and an AuoblA gene are integrated. According to the invention, a ophiobollin synthesis route is introduced into the saccharomyces cerevisiae in a heterologous manner for the first time, so that de novo synthesis of a ophiobollin skeleton compound, namely the ophiobollin F and an oxidation product of the ophiobollin F is realized; the synthesis of the ophiobolin F of 5.1 g / L is realized, which is also the highest sesterterpene heterologous synthesis yield reported at present; according to the invention, the ophiobolin 5-OH-21-CHO-opF with a new structure, which has excellent anti-cancer activity, is obtained through identification; the engineering strain of high-yield ophiobolin U is constructed, and the shake flask yield reaches 128.9 mg / L.

The present invention refers to a method for the preparation of a compound according to formula (45), its enantiomers, diastereomers, a racemic mixture, and the pharmaceutically acceptable salts thereof. Furthermore the invention refers to a compound of formula (45) prepared by said method for use in treatment of neurodegenerative disorders and to pharmaceutical compositions.

The present invention discloses terpene compounds derived from polar fungi, their preparation methods and applications, and respectively provides a calamenane-type sesquiterpene compound eutyacorane and a pimarane-type diterpene compound libertellenone Z derived from polar fungi. The structures are shown in the following formulas I and II respectively: The experimental results show that the two compounds have a better inhibition rate on the release of NO in the LPS-induced inflammatory model of mouse macrophages RAW264.7, providing a new type of choice for anti-inflammatory drugs.

The invention discloses a dihydro-beta-agilawood furan type sesquiterpenoids compound as shown in a formula (I) as well as a preparation method and application thereof. The compound provided by the invention can promote cartilage cell proliferation and reverse mRNA expression of symbolic genes Col2A1 and SOX-9 in cartilage cells under inflammation induction; meanwhile, the expression of inflammation-induced cartilage cell matrix metalloproteinase-13mRNA can be down-regulated, the degradation of cartilage cell extracellular matrix can be inhibited, and the cartilage cell metalloproteinase-13mRNA can be used for preparing products for preventing and / or treating diseases or symptoms caused by abnormal cartilage cell activity and / or improving bone and joint movement functions. # imgabs0 #

The application belongs to the field of enzyme genetic engineering, and particularly relates to application of a sesquiterpene synthase and a mutant thereof in synthesis of enantiomeric-drimenol compounds, wherein the sesquiterpene synthase is a sesquiterpene synthase from Chenopodium quinoa Willd., and includes CqDMDS1 or CqDMDS2; the mutant of the sesquiterpene synthase is a mutant CqDMDS1 in which an amino acid at position 443 of CqDMDS1 is mutated F443A / G The application realizes heterologous expression of the sesquiterpene synthase and the mutant thereof from Chenopodium quinoa Willd. in an Escherichia coli and a N. benthamiana expression system, catalyzes synthesis of (-)‑8,11-driman-diol or (+)‑drimenol. It is found through activity test that the sesquiterpene synthase from Chenopodium quinoa Willd. has a significant inhibitory effect on plant pathogenic fungi Phytophthora nicotianae, Phytophthora sojae and Fusarium graminearum. The sesquiterpene synthase and the mutant thereof and the biological preparation of the catalytic product have important significance for development of biological pesticides.

The invention belongs to the technical field of medicinal plant cultivation, and particularly provides a method for increasing the content of sesquiterpene glycoside in dendrobium nobile. The method comprises the step of applying a magnesium ion-containing solution with effective concentration at the growth stage of dendrobium nobile. Preferably, the magnesium ion-containing solution is a magnesium chloride solution, the application concentration is 10-500 mg / L, the magnesium ion-containing solution is applied in a foliage spraying or root irrigation mode, and the key period of application is the rapid growth period or the effective component accumulation period of the dendrobium nobile. The promotion effect of exogenous magnesium ions on biosynthesis of the sesquiterpene glycoside of the dendrobium nobile is disclosed for the first time, and the content of sesquiterpene glycoside components in the dendrobium nobile medicinal material can be remarkably increased under the condition that a basic cultivation mode is not changed, so that the medicinal potential and market value of immunoregulation, anti-inflammation and the like of the dendrobium nobile medicinal material are enhanced. The method is simple, efficient, low in cost, free of pollution and suitable for industrial application.

The invention relates to a sesquiterpenoid glycoside compound in Atractylodes lancea, and a preparation method and application thereof. The invention belongs to the technical field of medicines, and relates to a method for extracting and separating a sesquiterpenoid glycoside compound (1S, 5S, 7R, 10S)-10-hydroxy-deoxy split-ring atractylodes macrocephala delta-lactone-11-O-beta-D-glucopyranoside from rhizome of Atractylodes lancea by using normal phase silica gel, reverse phase silica gel, preparative high performance liquid chromatography and other technologies. In-vitro anti-tumor activity screening experiments show that the compound has good anti-tumor activity, including obvious inhibition effects on human lung cancer cells A549, human cervical cancer cells Hela and human liver cancer cells HepG-2. Therefore, the sesquiterpenoid glycoside compound has a prospect of being developed into antitumor drugs.

The invention provides a method for increasing the content of geranone and furandiene in zedoary turmeric oil, which comprises the following steps: activating secondary metabolism through moderate drought, low temperature or salt stress in a key growth period of zedoary turmeric, and promoting sesquiterpene accumulation; then, phosphorus-potassium fertilizer and zinc-boron trace elements are additionally applied, nitrogen fertilizer is reduced, and the component proportion is optimized; the preparation method comprises the following steps: washing, drying and crushing the rhizome of curcuma zedoary, adding water to infiltrate, extracting crude oil by water vapor, performing reduced pressure distillation and molecular distillation purification, finally filling nitrogen and sealing, and refrigerating at 2-4 DEG C and storing in a dark place. According to the method, the content and extraction rate of target components can be remarkably increased, purity is guaranteed, thermosensitive components are protected, pollution is reduced, and the storage period is prolonged.

The invention relates to the technical field of agriculture, and provides germacane type sesquiterpenoids in tithonia diversifolia as well as a preparation method and application thereof.The preparation method comprises the steps that stem and leaf parts of tithonia diversifolia are subjected to ethanol cold-soaking extraction, and an extracting solution is subjected to vacuum concentration to obtain a total extract; suspending the total extract with water, sequentially extracting with petroleum ether and ethyl acetate, and concentrating the extract liquor under reduced pressure to obtain each extraction layer; carrying out silica gel column chromatography separation on the ethyl acetate extraction layer, and combining to obtain six parts A-F; carrying out MCI column chromatography separation on the part D, and combining to obtain thirteen parts D1-D13; carrying out silica gel column chromatography separation on the D9 part, and combining to obtain fifteen parts D91-D915; and separating the D915 part by adopting HPLC (High Performance Liquid Chromatography) to prepare two germacane type sesquiterpenoids. The preparation method disclosed by the invention is simple and good in reproducibility, and the prepared germacane type sesquiterpenoids are high in purity and have specific herbicidal activity on foreign invasive plants such as ambrosia trifida and ambrosia esculenta.

The application belongs to the technical field of biology, and discloses application of CmPHL1 transcription factor in inhibition of citrus leaf oil cell formation and regulation of volatile substances. In view of the problem of insufficient research on existing citrus oil cell formation and essential oil synthesis, a pGBI-CmPHL1 overexpression vector is constructed, and positive strains are obtained by means of Agrobacterium-mediated transformation of citrus. Experiments prove that overexpression of CmPHL1 can significantly reduce the number of oil cells and the content of monoterpenes, sesquiterpenes and other volatile substances. CmPHL1 targets the molecular regulation network of oil cell development, accurately regulates and is heritable, provides a new gene target for citrus quality improvement and breeding, is in line with the direction of green agriculture, and has important economic and ecological benefits.

Sesterterpenoid compounds and an extract L01 of Leucosceptrum canum, and use thereof in treatment of psoriasis are provided, belonging to the technical field of natural medicinal chemistry. The sesterterpenoid compound of the Leucosceptrum canum has a structure shown in any one of Formulas 9 to 12, which shows a highly rare backbone type, exhibits anti-inflammatory and immunosuppressive activities, and can be used in preparation of an anti-inflammatory drug and an immunosuppressive drug. A Leucosceptrum canum extract L01 having compounds 1 to 12 as characteristic ingredients, and use thereof in preparation of an anti-inflammatory drug and an immunosuppressive drug are further provided.