30 results about "Fructosamine" patented technology

Methods whereby, by reference to fructosamine oxidase activity in blood plasma of a patient or patients, the risk of diabetes associated vascular complications can be assessed, candidate fructosamine oxidase inhibitors and/or antagonists can be identified or tested and the inhibition and/or antagonism of the fructosamine oxidase inhibition and/or antagonism of a patient can be assessed.

The method comprises: its standard solution uses the healthy human blood serum as base substance; the fructosamine measuring reagent box uses a combination of dual reagents R1 and R2; said R1 comprises NBT reaction component, pH is neutrality; said R2 is alkalinity potassium carbonate buffer solution; the ratio of R1,P2 to the sample under test is 40-50: 8-12: 3.

The invention relates to a process flow to produce a sesquiterpene inside a golden fungus fermentation liquid and the virtue of the sesquiterpene produced by the golden fungus to reduce the blood sugar, belonging to the biological fermentation and medicine field. The process flow is characterized in that the process flow applicant finds the fermentation liquid and the sesquiterpene produced by the production process are applied on a high blood sugar mouse through an oral administration route, the mouse significantly reduces the blood sugar and the fructosamine, which approves that the golden fungus fermentation liquid and the sesquiterpene are possessed of preparing a health care drink and medicine to reduce the blood sugar; the fermentation liquid color is orange to yellow-brown; the inhibitive rate to the ex vivo non-enzymic glycosylation is up to 72% to 90% with the fermentation liquid and 5mg/ml sesquiterpene. The process flow to produce a sesquiterpene inside a golden fungus fermentation liquid and the virtue of the sesquiterpene produced by the golden fungus to reduce the blood sugar has the advantages of not only creating a fire-new channel to further develop the golden fungus but also providing an ideal Chinese herbal medicine to reduce blood sugar.

The invention discloses a method for detecting an action mechanism of ursolic acid in inhibiting AGEs (advanced glycation end products), which includes the steps: firstly, simulating a non-enzymatic glycation system of body proteins; secondly, detecting inhibition of the ursolic acid against generation of fructosamine; thirdly, detecting inhibition of the ursolic acid against generation of AGEs; fourthly, processing data, statistically comparing inhibition of the ursolic acid against generation of the fructosamine to inhibition of the ursolic acid against generation of AGEs, and obtaining the action mechanism of the ursolic acid in inhibiting AGEs. The method can be used for simply and effectively detecting the action mechanism of the ursolic acid in inhibiting AGEs.

A method of measuring HbA1c is provided that, even with a whole blood sample after storage, measurement accuracy substantially equal to a whole blood sample right after collection can be maintained. Whole blood is stored in a presence of a glycolytic inhibitor and protease is added to the stored whole blood sample to cleave hemoglobin in the whole blood sample. Then a glycated part of a hemoglobin fragment thereby obtained is treated with fructosyl amine oxidase. Thereafter, a glycation degree of HbA1c is determined by measuring a redox reaction between the glycated part and the fructosyl amine oxidase. Further, instead of storage of the whole blood in a presence of the glycolytic inhibitor, a strong electrolyte substance such as KCl, K₂SO₄, KNO, NaCl, Na₂SO₄, NaNO, MgCl₂, MgSO₄, Mg(NO)₂, etc. is added to the whole blood after storage and a protease treatment is performed in a presence of the strong electrolyte substance. According to these methods, fluctuation in a measurement value of HbA1c due to storage of the whole blood can be avoided.

The invention discloses stereum hirsutum fermentation liquor and sesquiterpene which are produced by fermenting stereum hirsutum liquor, as well as applications thereof, and particularly relates to a production technology process of sesquiterpene cultured and obtained by the stereum hirsutum liquor, and the hypoglycemic efficiency of sesquiterpene. Repeated tests and study show that a culture solution produced by the production technology contains sesquiterpene, a mouse with hyperglycemia takes the culture solution orally, and then blood sugar and fructosamine of the mouse can be reduced remarkably, therefore, the stereum hirsutum culture solution is confirmed to have the application for preparing healthy drinks and medicines capable of reducing the blood sugar. The culture solution is liquor with the color ranging from aurantium to tawny, and the sesquiterpene content in the culture solution is 0.4 mg/mL-4.0 mg/mL. According to the invention, not only is a new way explored for the deep development and utilization of the stereum hirsutum, but also an ideal traditional Chinese medicine is provided for controlling the blood sugar.

Synthesis of fructosamine perfume compound and flavoring use of tobacco are disclosed. It is carried out by taking fructose and ammonia water as material and catalytic synthesizing in sodium hydrate-methyl alcohol solution system. It achieves low cost, simple synthetic process and high output. It can be used for additive of tobacco.

The invention discloses a synthesis method of 2-C-methyl-D-ribonic acid-1,4-lactone. The synthesis method comprises the following specific steps: step 1, isomerizing glucose in ethanol under an acidic condition to form fructose, and then enabling the fructose and N-methylbenzylamine to form fructosamine; step 2, crystallizing and purifying the fructosamine formed by the step 1 to obtain a solid; and step 3, enabling the fructosamine obtained by the step 2 to react under an alkaline condition, so as to obtain the 2-C-methyl-D-ribonic acid-1,4-lactone. According to the synthesis method disclosed by the invention, glucose is used as a raw material, so that the cost of the raw material is low; after the reaction is finished, an intermediate of the step 1 can be separated out through a crystallization manner, and a lot of uncertain impurities, generated in a glucose isomerization process, are removed, so that uncertain factors of the next step of reaction are greatly reduced; the method is simple in preparation process; conventional reaction reagents are adopted, so that the sources of the reagents are wide; and the reaction time is short, and the yield of products is high, so that the synthesis method is suitable for large-scale industrial production.

The invention discloses a calibrator for fructosamine detection and a detection kit applying the calibrator. The calibrator is bovine serum albumin. The invention also discloses a detection kit for determining fructosamine. The detection kit adopts the bovine serum albumin as the calibrator. According to the invention, on the basis of using 1-deoxy-1-morpholine fructose (DMF) as a first-grade standard substance and using a nitrotetrazolium blue chloride (NBT) reduction method to determine the fructosamine (FA), the method for determining the fructosamine (FA) by using bovine serum albumin as the calibrator (third grade) is reasonably established; the method provided by the invention can make up for defects of DMF as a standard substance in aspects of a molecular structure, a reaction rateand reaction condition sensitivity; and meanwhile, advantages of an accurate sample detection result, low cost and better transportation stability are possessed.

The invention discloses a multi-item detection all-in-one machine which comprises an electrochemical detection mechanism, a high-pressure liquid chromatography mechanism and a sample adding mechanism, the electrochemical detection mechanism is used for detecting the content of blood glucose and fructosamine, and the high-pressure liquid chromatography mechanism is used for detecting the content of glycosylated hemoglobin; the sample adding mechanism is connected with the high-pressure liquid chromatography mechanism, and the sample adding mechanism is used for conveying a sample solution to the high-pressure liquid chromatography mechanism and dripping the sample solution into the electrochemical detection mechanism. According to the multi-item detection all-in-one machine, the electrochemical detection mechanism and the high-pressure liquid chromatography mechanism are integrated together, so that the multi-item detection all-in-one machine can detect blood sugar, fructosamine and glycosylated hemoglobin at the same time, the situation that three detection items need to be carried out on three different instrument platforms is avoided, the detection efficiency is improved, and the detection cost is reduced. And meanwhile, repeated blood drawing operation on the patient when different items are detected is also avoided.

A method for predicting the degree of weight loss attainable by applying one or more dietary interventions to a subject, said method comprising determining the level of one or more biomarkers in one or more samples obtained from the subject, wherein the biomarkers are selected from fructosamine and factor VII.

The invention relates to a fructosamine decarboxylase carrier, a transgenic cell line and a genetically engineered bacterium for expressing fructosamine decarboxylase and application of fructosamine desaccharase, and belongs to the technical field of biological enzyme method production. The invention provides a method for catalyzing transfer of an amino group from an amino donor compound to an amino receptor compound, which comprises the following steps of: utilizing an enzyme (fructosamine desaccharase), or an expression vector or a cloning vector for expressing the enzyme, or a transgenic cell line for expressing the enzyme, or a genetically engineered bacterium for expressing the enzyme; and the catalytic amino group is transferred from the amino donor compound to the amino acceptor compound. The method provided by the invention has the advantages of sufficient substrate source, no limitation of raw materials, mild preparation conditions, small environmental pollution, high reaction specificity, few impurities in the system, easiness in downstream separation and purification, and low production cost.

The invention provides FN6K having temperature and pH stability and a method for measuring fructosyl lysine using the same. The method is characterized by reacting a sample to be tested with fructosamine-6-kinase having the following characteristics (a) to (f). (a) Producing a fructosyl lysine-6-phosphoric acid by phosphorylation of the fructosyl lysine in the presence of ATP: (b) specific activity: 10U/mg or more; (c) substrate specificity: reactivity against fructosyl valine is less than 5% when the reactivity to fructosyl lysine is assumed to be 100% or less, (d) molecular weight: 25kDa to35kDa; (e) pH stability: stable at 6.5 to 8.0; and (f) temperature stability: stable at 30 degrees centigrade.

The invention relates to a new anti-saccharification application of litsea coreana, an anti-saccharification product and a preparation method of the anti-saccharification product, and belongs to the technical field of plant extraction. On one hand, the invention provides the new anti-saccharification application of the litsea coreana. On the other hand, the invention provides the anti-saccharification product which is characterized by comprising an anti-saccharification active component; the action target of the anti-saccharification active component is selected from a group consisting of fructosamine, a protein carbonylation reaction pathway, AGEs, and a reaction pathway for crosslinking of a non-enzymatic glycosylation reaction intermediate product and protein; and the anti-saccharification active component comprises a litsea coreana extract. The third aspect of the invention provides a preparation method of the anti-saccharification product, which is characterized in that the litsea coreana extract is used as the anti-saccharification active component of the anti-saccharification product. Experiments prove that the litsea coreana extract has more excellent effects on inhibition of fructosamine, protein carbonylation and protein crosslinking than positive control aminoguanidine.

The invention discloses an apple virus vaccine. The apple virus vaccine is characterized by comprising ACLSV, ASGV virus strains, phosphoric acid, a filter, saccharose serving as a mother liquid and fructosamine. The apple virus vaccine disclosed by the invention has the beneficial effects that the situations that the conventional vaccine has an effect on only bacterial diseases but can do nothing for the virus are changed; the main component is non-pathogenic virus strains, can be multiplied for a long time and achieve an effect continuously; an injection mode is adopted, so that the effect is achieved more obviously.