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Oil sesquiterpene synthase and coding gene and application thereof

A technology of sesquiterpene synthase and encoding gene, which is used in application, genetic engineering, plant genetic improvement, etc.

Active Publication Date: 2018-12-11
RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biosynthetic genes for terpene synthases in Phoebe oleifera have not been isolated and identified
β-Caryophyllene is widely used in food flavors and flavors, etc., but its production is mainly through direct extraction from plants, and there are no reports of production by bioengineering methods

Method used

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  • Oil sesquiterpene synthase and coding gene and application thereof
  • Oil sesquiterpene synthase and coding gene and application thereof
  • Oil sesquiterpene synthase and coding gene and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] 1. Clone the oleifera sesquiterpene synthase gene SgSTPS1, construct a cloning vector and transform prokaryotic cells

[0026] RNA was extracted from the stem tissue of Phoebe oleifera, and cDNA was synthesized by reverse transcription using reverse transcriptase M-MLV. Using the cDNA as a template, the forward primer is: 5'-ATGGGGCGACCCACGGCAA-3', the reverse primer is: 5'-CTATTTGTTGACATCAATGGC-3', and PCR amplification is performed using Ex Taq DNA polymerase from TakaRa Company; the PCR conditions are: 94°C for 5min; 94°C for 30s, 55°C for 1min, 72°C for 1min30s, 35 cycles; 72°C for 10min. The PCR product was detected by 1% agarose gel electrophoresis, and the results were as follows: figure 1 as shown, figure 1 The M in the figure is the DNA marker, and the arrow is the fragment of the target gene SgSTPS1.

[0027] Use the method of agarose gel electrophoresis gel recovery kit to recover the target gene fragment, carry out TA cloning of the target fragment, conne...

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Abstract

The invention discloses an oil sesquiterpene synthase and a coding gene and application thereof. The invention provides a novel sesquiterpene synthase, namely oil sesquiterpene synthase SgSTPS1 and acoding gene thereof. The oil sesquiterpene synthase SgSTPS1 protein can produce an active sesquiterpene synthase after prokaryotic expression. The catalytic substrate FPP produces beta-caryophyllene,isocaryophyllene and alpha-caryophyllene, which can be used for mass production thereof.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to a oleifera sesquiterpene synthase and its coding gene and application. Background technique [0002] Sindora glabra Merr.ex de Wit belongs to the genus Sindora glabra Merr.ex de Wit. It is originally distributed in Hainan Province of my country. It is a tall tree in the tropics and subtropics. The most important feature of Phoebe oleifera is that resin oil can be secreted after the xylem of the trunk is damaged. Its flammability is similar to diesel oil, so it is also called "diesel tree". It has a wide range of uses in oil, medicine, ornamental and wood. development and utilization prospects. [0003] The components of Phoebe resin oil mainly include sesquiterpenoids, accounting for more than 70%, and diterpenes, accounting for more than 14%, of which α-copaene, β-caryophyllene and δ-junipene account for more than 30% More than 2 / 3 of semiterpenoi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/88C12N15/60C12N15/70C12N1/21C12P5/00C12R1/19
CPCC12N9/88C12N15/70C12P5/002C12Y402/03
Inventor 余纽杨锦昌李荣生尹光天邹文涛何畅
Owner RES INST OF TROPICAL FORESTRY CHINESE ACAD OF FORESTRY
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