48results about "Egg immunoglobulins" patented technology

The application provides a galactoside agglutinin 10 (Gal10) crystal and a preparation method and prepared antibody IgY and a pharmaceutical preparation thereof, and belongs to the technical field of medicines. The application uses the galactoside agglutinin 10 crystal as an antigen to prepare an egg yolk neutralizing antibody IgY capable of dissolving the Gal10 crystal, has the effects of improving rhinitis or asthma of a patient, preventing and treating rhinitis and asthma, and is safe, has no side effects, has a long-lasting curative effect, and has no drug resistance, thereby providing a safe, simple, hygienic, effective, economic and personalized treatment mode for prevention and treatment of rhinitis and asthma, and having a high market value.

The utility model relates to the technical field of egg yolk antibody extraction, and particularly discloses a separation and extraction device for antibodies in egg yolk, which comprises a treatment box, a bearing frame is fixedly mounted at the bottom end of the treatment box, a conveying pipe penetrates into one side of the top end of the treatment box, and a guide hopper is fixedly mounted at the lower part in the treatment box. A separation mechanism is arranged on one side of the conveying pipe at the top end of the treatment box; the separation mechanism comprises a motor, the motor is embedded in one side of the conveying pipe at the top end of the treatment box, a connecting plate is fixedly installed at the power output end of the motor, and a rotating cylinder is fixedly installed on the outer wall of the connecting plate. The communicating groove is communicated with the rotating cylinder, so that impurities such as residual residues and chippings can be discharged out of the rotating cylinder, and the situation that the quality of next separation and extraction is affected due to deterioration of the residual residues after a long time since the residues are difficult to clean due to adsorption is avoided.

The application relates to a yolk extraction device and a preparation method of egg yolk antibody. The extraction device comprises a workbench, a first linear module arranged on the workbench, a placing hole arranged on the first linear module, a pressing module arranged on the workbench and located above the first linear module, a lifting platform arranged on the workbench and located directly below the pressing module, an extraction shaft fixed at a first end of the lifting platform, a drill arranged on a second end of the extraction shaft and rotationally connected with the extraction shaft, a driving motor group arranged on the lifting platform, and two suction modules arranged on the lifting platform. The yolk extraction device and the preparation method of egg yolk antibody can directly extract egg white and egg yolk in the egg shell, so as to reduce the risk of contamination of the egg yolk liquid.

The invention relates to the technical field of biological product manufacturing, and particularly discloses an anti-shewanella-alginate egg yolk antibody as well as a preparation method and application thereof. The invention relates to an anti-shewanella algal egg yolk antibody, which is obtained by immunizing laying hens by using inactivated shewanella algal (RDSH-25070) thalli as antigens, collecting egg yolk liquid, separating and purifying, the shewanella algal (RDSH-25070) is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 14, 2025, is named as shewanella algal, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 14, 2025. The preservation number is CGMCC (China General Microbiological Culture Collection Center) The anti-shewanella-alginate egg yolk antibody disclosed by the invention has a strong inhibiting effect on shewanella alginate, can be effectively applied to preventing and treating diseases caused by the shewanella alginate for aquatic animals, and can be used for effectively retaining freshness and preventing corrosion of aquatic products at the same time.

Provided herein are methods of producing immunoglobulin Y (IgY) antibodies against monkeypox virus. The method includes immunizing hens with a peptide encoding at least one immunogenic domain of A29 fusion protein from the monkeypox virus and isolating the IgY antibodies against the A29 fusion protein from yolks of eggs laid by the immunized hens. Pharmaceutical compositions containing the IgY antibodies and methods of inhibiting or treating monkeypox virus infection are also provided.

The present invention relates to a method of producing a binder-toxin fusion protein comprising at least, one protein binder selected from the group consisting of an antibody, an antibody fragment or derivative retaining target binding capacity, or an antibody mimetic, optionally, a peptide linker, and at least one protein toxin or protein protoxin. The method comprises the steps of: contacting a plant cell or a whole plant with a nucleic acid construct comprising in operational linkage at least the following (A) at least one polynucleotide encoding for the protein binder, or a target binding chain or domain thereof, and either B1) a polynucleotide encoding for a cleavable peptide linker and a polynucleotide encoding for a protein toxin, or B2) a polynucleotide encoding for a protein protoxin, which protoxin comprises a cleavable domain for activation thereof, allowing the construct to integrate into the nucleus of the plant cell, or of one or more cells of the whole plant, and expressing the fusion protein encoded by the nucleic acid construct (Fig. 7).

This disclosure provides, among other things, a transgenic chicken. In some embodiments, the transgenic chicken comprises B cells in which the endogenous immunoglobulin heavy chain locus comprises: (a) a functional immunoglobulin heavy chain gene comprising a nucleic acid encoding a heavy chain variable domain in which the CDR3 is in the range of 30-60 amino acids in length and comprises at least 2 cysteine residues; and (b) a plurality of pseudogenes that are operably linked to said functional immunoglobulin heavy chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding the heavy chain variable domain of (a), wherein the pseudogenes are upstream or downstream of the functional immunoglobulin heavy chain gene.

The invention is suitable for the technical field of veterinary biological products, and provides a pasteurella egg yolk antibody for sheep and a preparation method thereof, and the preparation method comprises the following steps: S1, preparing an immunogen; s2, immunizing the hens; s3, crude extraction of an egg yolk antibody: separating egg yolk, collecting egg yolk liquid, adding a PBS buffer solution in proportion to fully dissolve the egg yolk, and standing and centrifuging to obtain a crude extract; s4, egg yolk antibody purification: precipitating the crude body fluid with an ammonium sulfate solution with the saturation degree of 38%, collecting antibody precipitate, dissolving with a PBS buffer solution, carrying out primary dialysis, then adding an ammonium sulfate solution with the saturation degree of 28%, carrying out secondary precipitation, and finally carrying out secondary dialysis to obtain a purified egg yolk antibody solution; and S5, antibody quality detection and split charging. The scheme has the advantages of comprehensive serotype coverage and strong protective force; the original animal is used for separating strains, so that the specificity is high; and the antibody has the advantages of high purity, low side reaction rate, quick response, no drug resistance, no residue and the like, and can be applied to prevention and treatment.

This disclosure provides, among other things, a transgenic chicken. In some embodiments, the transgenic chicken comprises B cells in which the endogenous immunoglobulin heavy chain locus comprises: (a) a functional immunoglobulin heavy chain gene comprising a nucleic acid encoding a heavy chain variable domain in which the CDR3 is in the range of 30-60 amino acids in length and comprises at least 2 cysteine residues; and (b) a plurality of pseudogenes that are operably linked to said functional immunoglobulin heavy chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding the heavy chain variable domain of (a), wherein the pseudogenes are upstream or downstream of the functional immunoglobulin heavy chain gene.

The application discloses an egg yolk antibody against Nocardiosis and a preparation method and application thereof, and relates to the technical field of biological medicines. A light chain sequence of the egg yolk antibody comprises a sequence shown in SEQ ID NO. 1, and a heavy chain sequence comprises a sequence shown in SEQ ID NO. 2. The application expands culture of pathogenic bacteria Nocardia, prepares an inactivated bacterin vaccine, and immunizes hens, so that the hens produce egg yolk antibodies against Nocardia through an immune response. After immunization, the application collects eggs, extracts the egg yolk antibodies against Nocardiosis from egg yolk by using a water dilution method. It is verified that the egg yolk antibodies have a strong binding capacity with Nocardia, can effectively inhibit Nocardia, and the titer can reach 1:64000 at the highest.

The invention discloses a preparation method of specific yolk immunoglobulin (IgY) for resisting gliadin. The preparation method comprises the following steps: immunizing and inoculating experimental laying hens, monitoring health behaviors, collecting eggs, and separating, extracting and purifying the IgY. The IgY prepared on the basis of the method can serve as a food base material to be added into low-gluten food (such as whole wheat bread and noodles), and the allergy risk caused by intake of a small amount of gluten is reduced through specific binding of the IgY and gliadin; patients are allowed to properly ingest gluten-containing food, diet diversity is improved, nutrient imbalance is avoided, and the life quality of the patients is improved. As the IgY has good thermal stability, the IgY purified by the method can still tolerate mild heating (such as baking and cooking) in food processing, and can be widely applied to various forms of products such as baked food, meal replacement powder, liquid beverage and the like; meanwhile, the acid-base stability of the antibody can ensure that the antibody keeps activity in a stomach environment after oral administration and finally reaches the intestinal tract to play a role, and the limitation that the conventional antibody is easy to digest and degrade after oral administration is broken through.

The invention belongs to the technical field of biological agents, and particularly relates to composite IgY antibody freeze-dried powder for pet oral hygiene and a preparation method and application thereof. Oral bacteria such as prevotella intermedia, porphyromonas gingivalis, streptococcus mutans, actinomyces viscosus and fusobacterium nucleatum are selected in a targeted manner, the bacteria are used for preparing a composite IgY antibody, the composite IgY antibody is combined with the freeze-drying agent optimized by the invention, and the antibody freeze-dried powder is prepared by immunizing laying hens, so that the range of the bacteria is wider, and the immunogenicity of the laying hens is improved. The compound IgY antibody obtained by the prepared compound IgY antibody freeze-dried powder is high in activity, and the compound IgY antibody freeze-dried powder has good re-dissolvability, stability, affordability, simplicity and convenience, can maintain titer at-20 DEG C for more than two years, and is convenient to store, transport and popularize. Compared with the prior art, the composite IgY antibody freeze-dried powder aims at wider flora, can effectively resist canine oral malignant bacteria, and can be used as an additive to be added into oral health products such as toothpaste and mouth wash.

The invention relates to the technical field of biological medicines, and particularly discloses an egg yolk antibody for resisting canine interleukin as well as a preparation method and application of the egg yolk antibody. The egg yolk antibody is prepared by taking immunocompetence antigen epitope peptide of canine interleukin-31 as an immunogen, the antigen epitope peptide is obtained by predicting key antigen epitopes of IL-31 through an Antigenic tool and performing series optimization, a coding gene of the antigen epitope peptide is optimized by an escherichia coli codon to construct a recombinant expression vector, and high-purity antigen is obtained by inducible expression and purification. A laying hen is immunized by the antigen, after basic immunization and two times of booster immunization, the egg yolk antibody is extracted from egg yolk and purified to obtain the egg yolk antibody, and the ELISA of the egg yolk antibody is obviously superior to that of an antibody prepared by commercial canine IL-31 protein immunization. The egg yolk antibody can be specifically combined with canine IL-31, the histamine level in serum of a canine with pruritus is remarkably reduced, the atopic dermatitis related pruritus symptoms are effectively relieved, an efficient and safe biological treatment preparation is provided for canine IL-31 mediated pruritus diseases, and the egg yolk antibody has wide clinical application prospects.

The invention discloses a technology for removing viruses from tetanus human immune globulin through a nano film. The process comprises the following steps: pretreating a feed liquid, so that the protein concentration of the feed liquid is 50-60g / L, the pH is 3.8-4.4, and the feed liquid contains 90-110g / L maltose and 50-150mmol / L sodium chloride; then, the feed liquid is subjected to two-stage filtration through a 0.1-micron nylon pre-filtration membrane bag with negative charges and a 20-nm regenerated cellulose (RC) virus removal membrane bag in sequence. According to the process, through optimizing the physical and chemical state of the feed liquid and the synergistic effect of the feed liquid and the functionalized filter membrane, the protein aggregation is effectively inhibited, the membrane pollution is reduced, the virus removal effect is ensured not to be lower than 4log10, the protein load of the RC membrane is remarkably improved to be more than 200L / m, and the product purity is more than or equal to 98%. The invention solves the technical problems of low flux and easy blockage of high-concentration immune globulin in small-aperture virus removal filtration, and is suitable for efficient, safe and large-scale production of blood products.

The invention relates to an anti-recombinant cat nerve growth factor egg yolk antibody as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing an antigen recombinant cat nerve growth factor by utilizing genetic engineering prokaryotic expression; immunizing laying hens by using the antigen recombinant cat nerve growth factors, and collecting eggs; the method comprises the following steps: cleaning eggs, separating yolk, mixing with normal saline to obtain a first mixed solution, adding chloroform into the first mixed solution, carrying out water bath to obtain a second mixed solution, centrifuging to obtain a yolk antibody extracting solution, and further purifying the yolk antibody extracting solution to obtain the yolk antibody, the amino acid of the recombinant cat nerve growth factor is the 113th-238th amino acid of the cat nerve growth factor, the gene sequence for coding the recombinant cat nerve growth factor is shown as SEQ ID NO: 8, and the prepared egg yolk antibody has excellent antigenicity, is more convenient to obtain, can effectively reduce the cost, is more beneficial to production, and has wide application prospects.

The present application relates to the technical field of immunology and biology, and particularly relates to an anti-colorectal cancer polyclonal antibody, a preparation method and application thereof, which comprises the following steps: inactivating HCT-116 colorectal cancer cells cultured in vitro or tumor cells cultured in primary culture after colorectal cancer tissue is lysed by four different methods, then performing magnetic stirring high-speed mixing with a mixed immunoadjuvant of white oil adjuvant and traditional Chinese medicine, obtaining a whole tumor cell vaccine, using the vaccine as an immunogen to immunize laying hens, regularly collecting eggs laid by the immunized laying hens, separating egg white and egg yolk, extracting egg yolk antibody, purifying, and obtaining an anti-colorectal cancer polyclonal antibody. The polyclonal antibody has high biological titer, and in-vivo and in-vitro experiments show that the polyclonal antibody has good anti-tumor effect. The present application uses complete tumor cells to obtain antigen epitopes of whole tumor cells, and the polyclonal antibody prepared by using the antigen epitopes can combine with multiple antigen binding sites of tumor cells, so that the chance of tumor escape is greatly reduced.

The present invention provides compositions, kits, and methods for the detection of host cell proteins (HCPs) in biological samples. In some embodiments, the present invention utilizes immunization of aves hosts with proteins derived from non-aves host cells to produce aves antibodies specific for non-aves HCPs.

The invention discloses a preparation method of an egg yolk antibody, which comprises the following steps of: preparing a vaccine composition from a porcine epidemic diarrhea virus strain with the microbial preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.46190, inoculating 90-180-day-old laying hens with the vaccine composition to prepare hyper-immune eggs, and extracting the egg yolk antibody in the hyper-immune eggs by using an octanoic acid extraction method. The egg yolk antibody can effectively prevent porcine epidemic diarrhea, is good in specificity, safe and effective, and has a clinical application prospect.

According to the present invention, the FPV-BJ strain (2 # seed virus) is adopted as the immunogen, and is inactivated to prepare the egg yolk antibody, the research results show that the egg yolk antibody with the FPV-HI titer of 1: 2048 can be obtained under the condition of the immunogen concentration of 105.0 TCID50 / ml, the obtained egg yolk antibody is higher than the existing reported level, and the egg yolk antibody can be further prepared into the feeding egg yolk antibody egg yolk powder for pet food, according to the present invention, the egg yolk antibody freeze-drying preparation or the egg yolk antibody injection preparation can be used as the drug, and the egg yolk antibody freeze-drying preparation is optimized, such that the treatment period and the treatment effect of the affected cat using the egg yolk antibody freeze-drying preparation or the egg yolk antibody injection preparation can be significantly improved; the symptom recovery time and the recovery time are shorter than those of sick cats without using the egg yolk antibody, so that the egg yolk antibody injection preparation prepared by the invention can effectively relieve clinical symptoms and greatly shorten the curing time.

A method of manufacturing ostrich antibodies may include immunizing mature female ostriches with a mixture solution comprising Clostridium difficile toxin A and toxin B, obtaining eggs from the immunized ostriches, and purifying egg yolk antibodies from the eggs.

The invention relates to the technical field of microorganisms, in particular to a highly pathogenic duck virus hepatitis III virus and an egg yolk antibody, preparation and application thereof. The virus strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 24, 2024, the preservation number is CGMCC No.46271, and the virus strain is named as RD240820B. The egg yolk antibody is prepared by using the highly pathogenic duck virus hepatitis III type virus strain as an antigen. The egg yolk antibody is applied to preparation of products for preventing or treating duck virus hepatitis III. In a virulence experiment on a duck embryo, the death rate of the virus strain reaches 80% after the duck embryo is inoculated for 36 hours, and the virus strain has strong pathogenicity. And the death rate within 60 hours after inoculation is 100%, and the strain has relatively strong pathogenicity. The death rate is still 60% when the diluent is 10-8, and the toxicity is strong. The ELD50 value of the strain is relatively low, which indicates that the strain has relatively strong pathogenicity to duck embryos. After the egg yolk antibody is injected, the protection rate reaches up to 97.6% after the diseased duck is treated, and the egg yolk antibody has a remarkable prevention and control effect.

The invention discloses an egg yolk antibody extraction device and process for pets, and relates to the technical field of egg yolk antibody extraction.The egg yolk antibody extraction device comprises a barrel, a movable disc is arranged at the top end of the barrel, a through hole is formed in the center of the movable disc, and second U-shaped blocks are fixedly connected to the positions, close to the left side and the right side, of the bottom of the movable disc; swing plates are hinged to inner cavities of second U-shaped blocks, first U-shaped blocks are hinged to the positions, close to the bottoms, of the edges of the outer sides of the swing plates, through mutual cooperation of a motor, a stirring rod, a vertical pipe, an L-shaped plate and other structures, yolk can be poured into the vertical pipe and enter the barrel, a filter screen removes yolk membranes on the yolk, and the yolk membranes on the yolk can be removed through the filter screen. The motor drives the vertical pipe to rotate through the first bevel gear and the second bevel gear, the vertical pipe drives the stirring rod to rotate, materials such as diluent are added while stirring is conducted, and when the motor is not needed for driving, the barrel is also independently rotated, and the barrel is matched with the stirring rod to stir the solution.

The invention relates to the technical field of biological medicine, and particularly discloses a preparation method of a collagen and anti-propionibacterium acnes gamma protein combined preparation, and the preparation method of the collagen and anti-propionibacterium acnes gamma protein combined preparation comprises the following steps: preparing anti-propionibacterium acnes gamma protein; adding water into the anti-propionibacterium acnes gamma protein and the recombinant III-type collagen, stirring and mixing, adding glycerol and a neutralizing agent, and mixing to obtain a uniform solution; the preparation method comprises the following steps: dispersing carbomer with water, adding a preservative and a humectant, mixing, finally adding the uniform solution, and mixing to obtain the required collagen and anti-propionibacterium acnes gamma protein combined preparation. The combined preparation prepared by the method can be used for effectively treating acnes, is remarkable in curative effect, does not generate drug resistance, is short in treatment cycle, can quickly eliminate skin damage, promote cell growth and adhesion and repair skin barriers, and does not generate new rash after healing.

The present disclosure belongs to the field of biotechnology, and particularly relates to a kind of egg yolk antibody specifically recognizing cat allergen FEL D1 and FEL D4 and its use.The present disclosure provides an egg yolk antibody composition, wherein the egg yolk antibody composition comprises egg yolk antibody specifically recognizing and neutralizing cat allergen FEL D1 and egg yolk antibody specifically recognizing and neutralizing cat allergen FEL D4.The egg yolk antibody composition can more effectively reduce the occurrence of allergic reaction, and can be applied using liquid preparation, directly acting on the allergen on the surface of the environment or cat body, and is more convenient to store and use.

The invention provides cat allergen protein self-assembled nanoparticles as well as a preparation method and application thereof, and belongs to the technical field of biology. The nanoparticles are formed by displaying cat allergen protein on the surface of a T7 bacteriophage through Spy Tag and Spy Catcher. The invention also provides a preparation method of the nanoparticle. The preparation method comprises the following steps: preparing the recombinant T7 bacteriophage nanoparticle with Spy Tag polypeptide displayed on the surface; preparing a fusion protein formed by the Spy Catcher protein and the cat allergen protein; and mixing the recombinant T7 bacteriophage nanoparticles with the fusion protein to obtain the nanoparticles. According to the invention, the Spy Tag is displayed on the surface of the T7 phage for the first time, and a covalent bond is spontaneously formed by the Spy Tag and the Spy Catcher-Fel d1 fusion protein, so that the efficient assembly of the allergen protein on the T7 phage is realized, the immunogenicity of Fel d1 is remarkably improved, and the development and application prospects are broad.

The application provides a novel HPV fusion protein antigen, a preparation method and application in preparation of polyclonal yolk antibody, which first connects core antigen peptides of human papilloma virus 16 subtype and HPV 18 subtype capsid protein L1 with Fc of immunoglobulin IgY and tail peptides of immunoglobulin IgM in series, and the above-mentioned recombinant fusion protein antigen is prepared by means of E. coli expression and inclusion body renaturation technology, which is beneficial to improving antigen peptide stability and presentation capacity, and the novel HPV fusion protein antigen is directly used for immunizing laying hens, after antibody production, the antibody is purified through steps such as salting-out and dialysis, and verification shows that the obtained yolk antibody can combine with L1 proteins of HPV16 and HPV18, and has the ability of preventing or treating HPV16 and HPV18 infection.

The invention discloses a preparation method and application of a GIIc type PEDV (Porcine Epidemic Diarrhea Virus) strain and a gastric acid resistant specific egg yolk antibody thereof, the PEDV strain is a GIIc type clinical predominant prevalent strain, and has good cell adaptability after plaque purification and domestication; the strain can be used for developing PEDV attenuated live vaccines, inactivated vaccines, novel genetic engineering vaccines, preventive and therapeutic antibodies and diagnostic reagents, and solves the technical problems that the existing vaccines are insufficient in protective power and the GIIc type PEDV clinical isolated strain is low in in-vitro culture titer; meanwhile, the invention discloses a core-shell nanoparticle antibody coating technology based on zein / carboxymethyl chitosan (Zein / CMCS), the PEDV egg yolk antibody IgY can be protected from being damaged by a strong acid environment and pepsase in the stomach of a pig, viruses in the intestinal tract are fully released and neutralized in the intestinal tract, PEDV infection is effectively prevented and treated, and the effect of preventing and treating porcine epidemic diarrhea is achieved. The problem that the neutralizing activity of the existing PEDV egg yolk antibody is sharply reduced after intragastric administration is solved.

The invention relates to the technical field of IgY antibodies, in particular to a preparation method and application of an anti-human papilloma virus IgY antibody, and the preparation method comprises the following steps: S1, preparing an immunogen; s2, carrying out stepped immunization; s3, collection and pretreatment of hyper-immune eggs: from 18-25 days after primary immunization, collecting eggs laid by the immunized hens, and after surface disinfection, taking yolk liquid for later use; s4, preliminarily extracting an antibody; s5, preliminarily concentrating and purifying the antibody; s6, fine purification of the antibody; s7, performing preparation treatment on the antibody; according to the present invention, the HPV L1 virus-like particle (VLP) immunogen strategy is adopted, the cross protection potential of the high immunogenicity of the L1 VLP is completely performed, the efficient adjuvant system is matched, the poultry is effectively induced to produce the high titer neutralizing antibody with wide cross reactivity, and the defects of limited single antigen targeting type and narrow protection range are overcome.

Nanoparticles comprising a composition comprising a polypeptide having a molecular weight greater than 50,000 g / mol (e.g., IgY antibody), wherein the composition is encapsulated in a material comprising a biocompatible biodegradable polymer, are provided. Also provided are methods of preparing these nanoparticles and the use of the nanoparticles as a therapeutic agent for treating a disease state.

The present application provides polyclonal antibodies for alleviating or treating pain in humans or animals, compositions comprising them, and uses thereof. In particular, the invention relates to a yolk IgY polyclonal antibody against pain mediator molecules NGF, CCL2 and CGRP and a composition thereof. The pain phenomenon of the OA sick dog can be relieved and the pain score can be reduced by independently orally taking the composition only containing one of the antibodies; by taking the composition containing two or three IgY antibodies, the pain relieving effect can be synergistically enhanced, and the highest pain relieving rate can reach 100%.