115results about "Egg immunoglobulins" patented technology

The invention relates to the technical field of biological medicine, in particular to cat allergen fusion protein, an mRNA immune preparation and application of the cat allergen fusion protein and the mRNA immune preparation. The amino acid sequence of the cat allergen fusion protein disclosed by the invention is as shown in SEQ ID NO.11. The cat allergen fusion protein provided by the invention is a fusion protein obtained by fusing key segments with strong sensitization of Fel d2 and Fel d4 proteins with Fel d1 full-length protein, the fusion protein can simultaneously cover main sensitization epitopes of Fel d1, Fel d2 and Fel d4, and the sensitization effect of cat allergens can be more comprehensively blocked. According to the cat allergen fusion protein provided by the invention, an mRNA immune preparation can be rapidly designed and synthesized, the expression of the cat allergen fusion protein can be accurately controlled in poultry bodies, the immunoreaction is more durable, and the prepared IgY antibody can effectively block allergic protein in cat oral saliva.

The invention discloses a preparation method of an anti-human TNF (Tumor Necrosis Factor) alpha egg yolk antibody. Relates to the technical field of biomedicine. The invention provides a sequence of the fusion protein, an egg yolk antibody and application. The fusion protein provided by the invention is constructed by combining a human TNF alpha sequence and an IgY Fc fragment, and efficient production is realized through a pichia pastoris expression system. The specific egg yolk antibody aiming at the human TNF alpha protein is generated by adopting an immune laying hen technology. The anti-TNF alpha egg yolk antibody prepared by the invention is expected to be converted into various clinical application forms by virtue of an innovative preparation process and excellent affinity, occurrence and development of inflammatory diseases are effectively inhibited and relieved, and a new biomedical solution is provided for diagnosis and treatment of the inflammatory diseases.

The disclosure relates to avian Immunoglobulin Y (IgY) antibodies and antigen binding fragments, variants, multimeric versions, or bispecifics thereof that specifically bind to an antigen (such as a human tumor associated antigen), as well as methods of making and using said antibodies and antigen binding fragments thereof in a variety of therapeutic, diagnostic and prophylactic indications.

This application discloses a feline allergen Fel d1 egg yolk antibody and its preparation method and application, comprising: obtaining PCR amplification products through PCR amplification; constructing a recombinant plasmid by ligating the PCR amplification products with pET-28a(+); transforming the recombinant plasmid into Rosetta competent cells to obtain transformants; obtaining recombinant antigens by extracting proteins from the transformants; obtaining a subunit vaccine by mixing and emulsifying the recombinant antigens with white oil adjuvant; administering the subunit vaccine to laying hens to obtain immunized egg yolk liquid; adding Tris-HCl buffer and polyethylene glycol to the egg yolk liquid and centrifuging to collect the protein aqueous phase; filtering the protein aqueous phase to obtain a filtrate, adding polyethylene glycol, mixing, centrifuging to collect the precipitate; mixing the precipitate with PBS buffer, adding ammonium sulfate, and centrifuging to obtain the egg yolk antibody. This invention solves the problem that the safety and economy of existing feline allergy treatment methods cannot be simultaneously achieved.

The invention discloses riemerella anatipestifer and application of the riemerella anatipestifer in an egg yolk antibody. The riemerella anatipestifer is named as BRA22A011, and the preservation number of the riemerella anatipestifer is CGMCC (China General Microbiological Culture Collection Center) No.45887. The invention also discloses an inactivated vaccine, wherein the antigen in the inactivated vaccine contains the inactivated riemerella anatipestifer BRA22A011. The invention further discloses an egg yolk antibody, and the egg yolk antibody is prepared by using the riemerella anatipestifer BRA22A011 as an antigen. The use mode of the egg yolk antibody is intramuscular injection inoculation, and the injection dosage is 0.5 mL. The riemerella anatipestifer BRA22A011 has high pathogenicity and strong immunogenicity, and the egg yolk antibody prepared from the riemerella anatipestifer BRA22A011 can replace antibiotics and vaccines, is safe, free of residues, free of drug resistance and environment-friendly, and has a good prospect in clinical application of preventing and treating infectious serositis of ducks. The riemerella anatipestifer BRA22A011 and the Escherichia coli BE-20261 are adopted for preparing the egg yolk antibody together, and the prevention and treatment effects of the egg yolk antibody are more obvious.

The application provides a galactoside agglutinin 10 (Gal10) crystal and a preparation method and prepared antibody IgY and a pharmaceutical preparation thereof, and belongs to the technical field of medicines. The application uses the galactoside agglutinin 10 crystal as an antigen to prepare an egg yolk neutralizing antibody IgY capable of dissolving the Gal10 crystal, has the effects of improving rhinitis or asthma of a patient, preventing and treating rhinitis and asthma, and is safe, has no side effects, has a long-lasting curative effect, and has no drug resistance, thereby providing a safe, simple, hygienic, effective, economic and personalized treatment mode for prevention and treatment of rhinitis and asthma, and having a high market value.

The utility model relates to the technical field of egg yolk antibody extraction, and particularly discloses a separation and extraction device for antibodies in egg yolk, which comprises a treatment box, a bearing frame is fixedly mounted at the bottom end of the treatment box, a conveying pipe penetrates into one side of the top end of the treatment box, and a guide hopper is fixedly mounted at the lower part in the treatment box. A separation mechanism is arranged on one side of the conveying pipe at the top end of the treatment box; the separation mechanism comprises a motor, the motor is embedded in one side of the conveying pipe at the top end of the treatment box, a connecting plate is fixedly installed at the power output end of the motor, and a rotating cylinder is fixedly installed on the outer wall of the connecting plate. The communicating groove is communicated with the rotating cylinder, so that impurities such as residual residues and chippings can be discharged out of the rotating cylinder, and the situation that the quality of next separation and extraction is affected due to deterioration of the residual residues after a long time since the residues are difficult to clean due to adsorption is avoided.

The invention discloses an egg yolk globulin crude extraction method, extraction process and extraction equipment, and relates to the technical field of egg yolk globulin extraction.The process comprises the following steps that S1, egg yolk liquid is poured into a mold to be subjected to liquid nitrogen quick freezing, demolding is conducted, and the egg yolk liquid is smashed into particles; s2, mixing and stirring precooled acetone and the frozen egg yolk particles, centrifuging, and removing lipid precipitates on the upper layer; s3, adding the pre-cooled PBS, mixing, stirring and centrifuging, taking supernate, and carrying out treatment at the temperature of 0-5 DEG C; s4, slowly adding ammonium sulfate until the saturation degree is 30-40%, standing for 2 hours in an environment of 0-5 DEG C, then performing centrifugal treatment, taking precipitate, and redissolving the precipitate with PBS to obtain a redissolved solution; s5, performing ultrafiltration concentration on the reconstitution fluid by using an ultrafiltration membrane with the molecular weight cutoff of 100-200kDa; s6, carrying out ion exchange layer and hydrophobic layer chromatography treatment in sequence; and S7, carrying out pre-freezing in an environment of-80 DEG C, and then carrying out vacuum freeze-drying treatment. The method has the effect of improving the comprehensive benefit of extracting high-purity and high-activity egg yolk globulin.

The application relates to a yolk extraction device and a preparation method of egg yolk antibody. The extraction device comprises a workbench, a first linear module arranged on the workbench, a placing hole arranged on the first linear module, a pressing module arranged on the workbench and located above the first linear module, a lifting platform arranged on the workbench and located directly below the pressing module, an extraction shaft fixed at a first end of the lifting platform, a drill arranged on a second end of the extraction shaft and rotationally connected with the extraction shaft, a driving motor group arranged on the lifting platform, and two suction modules arranged on the lifting platform. The yolk extraction device and the preparation method of egg yolk antibody can directly extract egg white and egg yolk in the egg shell, so as to reduce the risk of contamination of the egg yolk liquid.

The invention relates to the technical field of biological product manufacturing, and particularly discloses an anti-shewanella-alginate egg yolk antibody as well as a preparation method and application thereof. The invention relates to an anti-shewanella algal egg yolk antibody, which is obtained by immunizing laying hens by using inactivated shewanella algal (RDSH-25070) thalli as antigens, collecting egg yolk liquid, separating and purifying, the shewanella algal (RDSH-25070) is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 14, 2025, is named as shewanella algal, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 14, 2025. The preservation number is CGMCC (China General Microbiological Culture Collection Center) The anti-shewanella-alginate egg yolk antibody disclosed by the invention has a strong inhibiting effect on shewanella alginate, can be effectively applied to preventing and treating diseases caused by the shewanella alginate for aquatic animals, and can be used for effectively retaining freshness and preventing corrosion of aquatic products at the same time.

Provided herein are methods of producing immunoglobulin Y (IgY) antibodies against monkeypox virus. The method includes immunizing hens with a peptide encoding at least one immunogenic domain of A29 fusion protein from the monkeypox virus and isolating the IgY antibodies against the A29 fusion protein from yolks of eggs laid by the immunized hens. Pharmaceutical compositions containing the IgY antibodies and methods of inhibiting or treating monkeypox virus infection are also provided.

The invention relates to the field of biological products, and particularly discloses a goose astrovirus, a goose astrovirus egg yolk antibody as well as a preparation method and application of the goose astrovirus egg yolk antibody. The invention relates to a goose astrovirus, which is named as RD240629A, is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No: 46197, and the preservation date is September 23, 2024. The goose astrovirus is named as RD240629A, and the goose astrovirus is named as RD240629A and is preserved in the China General Microbiological Culture Collection Center (CGMCC). The goose astrovirus can be used for preparing the egg yolk antibody for preventing gout of goslings, the egg yolk antibody is good in safety, no local or whole-body adverse reaction caused by antibody injection occurs, infection of the novel goose astrovirus can be effectively prevented and / or treated, and the anti-gout effect is good. The prepared egg yolk antibody is evaluated by adopting antibody titer determination and a challenge protection test, the titer of the egg yolk antibody is not lower than 1: 256, and the egg yolk antibody has a good commercial development prospect.

A composition and method for therapeutic or prophylactic treatment of mammals includes a mixture of IgY antibodies derived from one or more eggs laid by one or more avian species, wherein each of the one or more avian species have been vaccinated with one or more of the plurality of antigens. The mixture of IgY antibodies can be combined with a protective material that includes colostrum. In some embodiments the composition can also include IgA and IgM antibodies.

The present invention discloses an egg yolk antibody against Muscovy duck reovirus of goose origin, its preparation method and application, belonging to the technical field of veterinary biological products. The egg yolk antibody is prepared by immunizing poultry with a vaccine prepared using the inactivated Muscovy duck reovirus of goose origin with the preservation number CCTCC NO: V202491 as an antigen, and then extracting and purifying it from the egg yolk of highly immunized poultry eggs. Using the Muscovy duck reovirus of goose origin with the preservation number CCTCC NO: V202491 as a vaccine production strain to prepare an inactivated vaccine to immunize poultry can efficiently obtain the egg yolk antibody. This egg yolk antibody has good safety, high neutralization titer, and has a good prevention and treatment effect against the infection of Muscovy duck reovirus of goose origin, and has broad prospects for commercial development.

The invention discloses a method for producing feed granules containing antiviral proteins for protecting aquatic animals from viral infestation by passive immunization. The particles contain an effective amount of an active antiviral protein that is incorporated into the basal feed raw material together with a stabilizer, a gelling agent and a bioavailability enhancer. The antiviral protein is an IgY antibody contained in a hyper-immune egg powder obtained from an egg produced from an avian species exposed to an antigen associated with viral infection. In order to protect shrimps from white spot syndrome virus (WSSV), the antigens consist of proteins made of active portions of the VP28 and VP19 sequences of the WSSV. In one embodiment, the stabilizer is sodium alginate, the gelling agent is carboxymethyl cellulose, and the bioavailability enhancer is piperine. In one embodiment, the active protein and related supplements are incorporated throughout the feed pellet, and in another embodiment, it is sprayed on the surface of the feed pellet.

Nanoparticles comprising a composition comprising a polypeptide having a molecular weight greater than 50,000 g / mol (e.g., IgY antibody), wherein the composition is encapsulated in a material comprising a biocompatible biodegradable polymer, are provided. Also provided are methods of preparing these nanoparticles and the use of the nanoparticles as a therapeutic agent for treating a disease state.

The present invention relates to a method of producing a binder-toxin fusion protein comprising at least, one protein binder selected from the group consisting of an antibody, an antibody fragment or derivative retaining target binding capacity, or an antibody mimetic, optionally, a peptide linker, and at least one protein toxin or protein protoxin. The method comprises the steps of: contacting a plant cell or a whole plant with a nucleic acid construct comprising in operational linkage at least the following (A) at least one polynucleotide encoding for the protein binder, or a target binding chain or domain thereof, and either B1) a polynucleotide encoding for a cleavable peptide linker and a polynucleotide encoding for a protein toxin, or B2) a polynucleotide encoding for a protein protoxin, which protoxin comprises a cleavable domain for activation thereof, allowing the construct to integrate into the nucleus of the plant cell, or of one or more cells of the whole plant, and expressing the fusion protein encoded by the nucleic acid construct (Fig. 7).

The invention provides a canine coronavirus strain as well as an inactivated vaccine and application thereof. The canine coronavirus strain is named as CCoV2015, and the genome full-length cDNA sequence of the canine coronavirus strain is as shown in SEQ ID NO. 1. The canine coronavirus strain is collected from intestinal tissues of naturally diseased dogs, sequence comparative analysis shows that the strain is a novel canine coronavirus strain, the infection ability of the strain is high, the strain is highly homologous with canine coronavirus strains separated in recent years, and the canine coronavirus strain is inactivated to prepare the canine coronavirus vaccine. Meanwhile, the canine coronavirus vaccine also fills the blank of a single canine coronavirus vaccine, and plays a very important role in prevention of canine coronavirus.

This disclosure provides, among other things, a transgenic chicken. In some embodiments, the transgenic chicken comprises B cells in which the endogenous immunoglobulin heavy chain locus comprises: (a) a functional immunoglobulin heavy chain gene comprising a nucleic acid encoding a heavy chain variable domain in which the CDR3 is in the range of 30-60 amino acids in length and comprises at least 2 cysteine residues; and (b) a plurality of pseudogenes that are operably linked to said functional immunoglobulin heavy chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding the heavy chain variable domain of (a), wherein the pseudogenes are upstream or downstream of the functional immunoglobulin heavy chain gene.

The invention discloses a preparation method and application of a composite antibody IgY for preventing and treating acne, and belongs to the technical field of cosmetics. The preparation method comprises the following steps: (1) preparing immunogens: respectively mixing inactivated propionibacterium acnes liquid, staphylococcus epidermidis liquid, staphylococcus aureus liquid and escherichia coli liquid with an ISA 78 adjuvant to obtain the immunogens of the four bacteria; (2) immunizing: respectively injecting immunogens of the four bacteria to laying hens, and collecting to obtain immunized eggs of the four bacteria; and (3) collection and preparation of a composite antibody IgY: mixing immunized eggs of the four bacteria, separating yolk, and carrying out acid treatment and impurity removal to obtain the composite antibody IgY. The prepared composite antibody IgY is high in titer, has the effect of preventing and treating acne and acne infection, is high in safety and remarkable in curative effect, and provides a new treatment strategy for preventing and treating acne.

The invention is suitable for the technical field of veterinary biological products, and provides a pasteurella egg yolk antibody for sheep and a preparation method thereof, and the preparation method comprises the following steps: S1, preparing an immunogen; s2, immunizing the hens; s3, crude extraction of an egg yolk antibody: separating egg yolk, collecting egg yolk liquid, adding a PBS buffer solution in proportion to fully dissolve the egg yolk, and standing and centrifuging to obtain a crude extract; s4, egg yolk antibody purification: precipitating the crude body fluid with an ammonium sulfate solution with the saturation degree of 38%, collecting antibody precipitate, dissolving with a PBS buffer solution, carrying out primary dialysis, then adding an ammonium sulfate solution with the saturation degree of 28%, carrying out secondary precipitation, and finally carrying out secondary dialysis to obtain a purified egg yolk antibody solution; and S5, antibody quality detection and split charging. The scheme has the advantages of comprehensive serotype coverage and strong protective force; the original animal is used for separating strains, so that the specificity is high; and the antibody has the advantages of high purity, low side reaction rate, quick response, no drug resistance, no residue and the like, and can be applied to prevention and treatment.

This disclosure provides, among other things, a transgenic chicken. In some embodiments, the transgenic chicken comprises B cells in which the endogenous immunoglobulin heavy chain locus comprises: (a) a functional immunoglobulin heavy chain gene comprising a nucleic acid encoding a heavy chain variable domain in which the CDR3 is in the range of 30-60 amino acids in length and comprises at least 2 cysteine residues; and (b) a plurality of pseudogenes that are operably linked to said functional immunoglobulin heavy chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding the heavy chain variable domain of (a), wherein the pseudogenes are upstream or downstream of the functional immunoglobulin heavy chain gene.

The utility model relates to the field of egg yolk antibody preparation equipment, in particular to an egg yolk antibody filtering and clarifying device which comprises a steel barrel and a filter bag arranged in the steel barrel, a rocker is rotationally arranged on the inner side wall of the steel barrel, and one end of the rocker can be close to or away from the filter bag by rotating the rocker. The steel drum is further provided with a driving assembly used for driving the rocker to rotate in a reciprocating mode. The device has the effects of reducing the blockage of the filter bag and improving the production efficiency of the egg yolk antibody.

The application discloses an egg yolk antibody against Nocardiosis and a preparation method and application thereof, and relates to the technical field of biological medicines. A light chain sequence of the egg yolk antibody comprises a sequence shown in SEQ ID NO. 1, and a heavy chain sequence comprises a sequence shown in SEQ ID NO. 2. The application expands culture of pathogenic bacteria Nocardia, prepares an inactivated bacterin vaccine, and immunizes hens, so that the hens produce egg yolk antibodies against Nocardia through an immune response. After immunization, the application collects eggs, extracts the egg yolk antibodies against Nocardiosis from egg yolk by using a water dilution method. It is verified that the egg yolk antibodies have a strong binding capacity with Nocardia, can effectively inhibit Nocardia, and the titer can reach 1:64000 at the highest.

In one aspect, the present disclosure is directed to a method for preventing or treating a coronavirus infection in a subject in need thereof, comprising administering to the subject a therapeutically effective amount of a hyperimmunized egg product obtained from an egg-producing animal, thereby preventing or treating coronavirus infection in the subject, wherein the hyperimmunized egg product comprises a therapeutically effective amount of one or more antibodies to the coronavirus. The present disclosure is also directed to hyperimmunized eggs and egg products produced by an animal that has been hyperimmunized with an antigen selected from i) a spike (S) protein, an S1 subunit protein, an S2 subunit protein, a receptor binding domain (RBD), and an immunogenic fragment thereof; ii) a nucleocapsid (N) protein, and an immunogenic fragment thereof; iii) a human ACE2 receptor protein, and an immunogenic fragment thereof; and iv) a human coronavirus selected from the group consisting of SARS-CoV, MERS-CoV, SARS-CoV-2, HCoV-HKU1, HCoV-NL63, HCoV-OC43 and HCoV-229E. Methods of preparing the hyperimmunized eggs and egg products are also disclosed.

The invention provides a preparation method of a grouper nervous necrosis virus protein antigen and an egg yolk antibody, which comprises the following steps of: obtaining a grouper nervous necrosis virus capsid protein gene sequence, recombining the grouper nervous necrosis virus capsid protein gene sequence into a pET-28a carrier, and introducing into escherichia coli for expression to obtain the grouper nervous necrosis virus protein antigen. The grouper nervous necrosis virus capsid protein antigen obtained after purification, concentration, folding and dialysis is pseudovirus with an icosahedron symmetrical structure, after laying hens are immunized by the pseudovirus, eggs of the laying hens are collected, and egg yolk antibodies are extracted and applied to immune protection of groupers. The obtained antigen is high in immunogenicity, the obtained antibody has excellent titer, has practical value for preventing and treating nervous necrosis virus of grouper, is high in safety and low in cost, and can effectively reduce economic loss of aquaculture.

The invention discloses a preparation method of specific yolk immunoglobulin (IgY) for resisting gliadin. The preparation method comprises the following steps: immunizing and inoculating experimental laying hens, monitoring health behaviors, collecting eggs, and separating, extracting and purifying the IgY. The IgY prepared on the basis of the method can serve as a food base material to be added into low-gluten food (such as whole wheat bread and noodles), and the allergy risk caused by intake of a small amount of gluten is reduced through specific binding of the IgY and gliadin; patients are allowed to properly ingest gluten-containing food, diet diversity is improved, nutrient imbalance is avoided, and the life quality of the patients is improved. As the IgY has good thermal stability, the IgY purified by the method can still tolerate mild heating (such as baking and cooking) in food processing, and can be widely applied to various forms of products such as baked food, meal replacement powder, liquid beverage and the like; meanwhile, the acid-base stability of the antibody can ensure that the antibody keeps activity in a stomach environment after oral administration and finally reaches the intestinal tract to play a role, and the limitation that the conventional antibody is easy to digest and degrade after oral administration is broken through.

The invention belongs to the technical field of biological agents, and particularly relates to composite IgY antibody freeze-dried powder for pet oral hygiene and a preparation method and application thereof. Oral bacteria such as prevotella intermedia, porphyromonas gingivalis, streptococcus mutans, actinomyces viscosus and fusobacterium nucleatum are selected in a targeted manner, the bacteria are used for preparing a composite IgY antibody, the composite IgY antibody is combined with the freeze-drying agent optimized by the invention, and the antibody freeze-dried powder is prepared by immunizing laying hens, so that the range of the bacteria is wider, and the immunogenicity of the laying hens is improved. The compound IgY antibody obtained by the prepared compound IgY antibody freeze-dried powder is high in activity, and the compound IgY antibody freeze-dried powder has good re-dissolvability, stability, affordability, simplicity and convenience, can maintain titer at-20 DEG C for more than two years, and is convenient to store, transport and popularize. Compared with the prior art, the composite IgY antibody freeze-dried powder aims at wider flora, can effectively resist canine oral malignant bacteria, and can be used as an additive to be added into oral health products such as toothpaste and mouth wash.

The invention relates to the technical field of biological medicines, and particularly discloses an egg yolk antibody for resisting canine interleukin as well as a preparation method and application of the egg yolk antibody. The egg yolk antibody is prepared by taking immunocompetence antigen epitope peptide of canine interleukin-31 as an immunogen, the antigen epitope peptide is obtained by predicting key antigen epitopes of IL-31 through an Antigenic tool and performing series optimization, a coding gene of the antigen epitope peptide is optimized by an escherichia coli codon to construct a recombinant expression vector, and high-purity antigen is obtained by inducible expression and purification. A laying hen is immunized by the antigen, after basic immunization and two times of booster immunization, the egg yolk antibody is extracted from egg yolk and purified to obtain the egg yolk antibody, and the ELISA of the egg yolk antibody is obviously superior to that of an antibody prepared by commercial canine IL-31 protein immunization. The egg yolk antibody can be specifically combined with canine IL-31, the histamine level in serum of a canine with pruritus is remarkably reduced, the atopic dermatitis related pruritus symptoms are effectively relieved, an efficient and safe biological treatment preparation is provided for canine IL-31 mediated pruritus diseases, and the egg yolk antibody has wide clinical application prospects.

This disclosure provides, among other things, a transgenic chicken. In some embodiments, the transgenic chicken comprises B cells in which the endogenous immunoglobulin heavy chain locus comprises: (a) a functional immunoglobulin heavy chain gene comprising a nucleic acid encoding a heavy chain variable domain in which the CDR3 is in the range of 30-60 amino acids in length and comprises at least 2 cysteine residues; and (b) a plurality of pseudogenes that are operably linked to said functional immunoglobulin heavy chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding the heavy chain variable domain of (a), wherein the pseudogenes are upstream or downstream of the functional immunoglobulin heavy chain gene.