159results about "Egg immunoglobulins" patented technology

The invention relates to the technical field of biological medicine, in particular to cat allergen fusion protein, an mRNA immune preparation and application of the cat allergen fusion protein and the mRNA immune preparation. The amino acid sequence of the cat allergen fusion protein disclosed by the invention is as shown in SEQ ID NO.11. The cat allergen fusion protein provided by the invention is a fusion protein obtained by fusing key segments with strong sensitization of Fel d2 and Fel d4 proteins with Fel d1 full-length protein, the fusion protein can simultaneously cover main sensitization epitopes of Fel d1, Fel d2 and Fel d4, and the sensitization effect of cat allergens can be more comprehensively blocked. According to the cat allergen fusion protein provided by the invention, an mRNA immune preparation can be rapidly designed and synthesized, the expression of the cat allergen fusion protein can be accurately controlled in poultry bodies, the immunoreaction is more durable, and the prepared IgY antibody can effectively block allergic protein in cat oral saliva.

The invention belongs to the technical field of biology, and particularly relates to an egg yolk antibody for specifically recognizing cat allergens FEL D1 and FEL D4 and application of the egg yolk antibody. The invention provides an egg yolk antibody composition, and the egg yolk antibody composition comprises an egg yolk antibody capable of specifically recognizing and neutralizing a cat allergen FEL D1 and an egg yolk antibody capable of specifically recognizing and neutralizing a cat allergen FEL D4. The egg yolk antibody composition can more effectively reduce the occurrence of anaphylactic reaction, can be applied by adopting a liquid preparation, directly acts on allergens in the environment or on the surface of a cat body, and is more convenient to store and use.

The invention discloses a preparation method of an anti-human TNF (Tumor Necrosis Factor) alpha egg yolk antibody. Relates to the technical field of biomedicine. The invention provides a sequence of the fusion protein, an egg yolk antibody and application. The fusion protein provided by the invention is constructed by combining a human TNF alpha sequence and an IgY Fc fragment, and efficient production is realized through a pichia pastoris expression system. The specific egg yolk antibody aiming at the human TNF alpha protein is generated by adopting an immune laying hen technology. The anti-TNF alpha egg yolk antibody prepared by the invention is expected to be converted into various clinical application forms by virtue of an innovative preparation process and excellent affinity, occurrence and development of inflammatory diseases are effectively inhibited and relieved, and a new biomedical solution is provided for diagnosis and treatment of the inflammatory diseases.

The invention relates to the technical field of biological agents, in particular to a goose astrovirus egg yolk antibody and a preparation method and application thereof. The invention provides a goose astrovirus egg yolk antibody. The goose astrovirus egg yolk antibody is prepared by adopting an inactivated vaccine as an antigen, wherein the inactivated vaccine is prepared from a goose astrovirus GAstV / SD / 36-95P strain with the preservation number of CCTCC (China Center For Type Culture Collection) NO: V202445. The neutralizing titer of the goose astrovirus egg yolk antibody provided by the invention is high and can reach 10.5 log2, after goslings are infected by the goose astrovirus, the goslings can be protected from disease attack and death by injecting the egg yolk antibody, and the protection rate of the egg yolk antibody can reach 100%.

The invention relates to the technical field of bioengineering, and particularly discloses a chicken infectious anemia virus, a chicken infectious anemia virus egg yolk antibody and a preparation method and application thereof. The invention relates to a chicken infectious anemia virus, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and is named as the chicken infectious anemia virus RD240101A, the preservation date is September 23, 2024, and the preservation number is CGMCC NO.46198. The chicken infectious anemia virus has the advantages that the chicken infectious anemia virus is named as the chicken infectious anemia virus RD240101A; the chicken infectious anemia virus egg yolk antibody is prepared by taking an inactivated vaccine prepared from the chicken infectious anemia virus RD240101A as an immunogen, is high in specificity and good in safety, does not have any local or whole-body adverse reaction caused by injection of the antibody after being inoculated to chicks, can provide effective immune protection for chicken flocks, and can be used for preparing the chicken infectious anemia virus egg yolk antibody. And the method has a good commercial development prospect.

The disclosure relates to avian Immunoglobulin Y (IgY) antibodies and antigen binding fragments, variants, multimeric versions, or bispecifics thereof that specifically bind to an antigen (such as a human tumor associated antigen), as well as methods of making and using said antibodies and antigen binding fragments thereof in a variety of therapeutic, diagnostic and prophylactic indications.

The invention belongs to the technical field of bioengineering, and particularly relates to a cat hair allergy recombinant fusion protein as well as a preparation method and application thereof. The method comprises the following steps: analyzing and screening B cell epitopes of the cat hair allergic protein, carrying out series fusion expression on Feld1, Feld3, Feld4, Feld7 and Feld8 and the B cell epitopes of the allergic protein, optimizing escherichia coli codons, and realizing high-efficiency expression in an escherichia coli expression system; the fusion expression scheme not only has higher protein expression, but also enhances the immunogenicity due to the connection with the own B cell epitope. A plurality of cat hair allergy recombinant fusion proteins with strong immunogenicity are adopted to carry out subcutaneous immunization on laying hens to obtain corresponding high-titer broad-spectrum egg yolk antibodies, allergy proteins can be effectively neutralized, the egg yolk antibodies are used for preparing functional cat food or additives, and allergic reactions of animals and contact personnel can be reduced.

The invention relates to the technical field of antibodies, in particular to a quadruple egg yolk antibody for cats as well as a preparation method and application of the quadruple egg yolk antibody. The quadruple egg yolk antibody for cats, provided by the invention, comprises egg yolk antibodies aiming at four viruses, namely cat parvovirus, cat herpes virus type I, cat calicivirus and cat coronavirus, can specifically neutralize the viruses, has relatively high neutralizing antibody titer, and can be used for preventing and treating infection of the viruses and diseases caused by the infection. The quadruple egg yolk antibody for cats provided by the invention has the advantages that the preparation method is simple and efficient, the egg yolk antibody is high in yield and purity, the prepared antibody is high in titer and low in cost, large-scale production is easy to realize and the like, and the quadruple egg yolk antibody has a wide application prospect.

The invention relates to the field of microorganisms, and particularly discloses a highly pathogenic gosling plague virus and a preparation method of an egg yolk antibody of the highly pathogenic gosling plague virus. The invention relates to a gosling plague virus, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), is named as the gosling plague virus RD2311B-E21, is preserved on April 22, 2024, and has the preservation number of CGMCC NO.45964. The gosling plague virus is named as the gosling plague virus RD2311B-E21. The preparation method of the egg yolk antibody comprises the following steps: proliferating and inactivating gosling plague virus RD2311B-E21 to prepare an immunogen; respectively injecting the immunogen into the laying hens for four times to obtain four-immunized eggs; the preparation method comprises the following steps: soaking a tetra-immunized egg in a bromogeramine solution, performing spray fumigation with glutaraldehyde, then separating yolk, treating the yolk with disodium hydrogen phosphate to obtain a turbid solution, and performing acidification extraction, degreasing with octanoic acid, degerming filtration, virus removal by ultrafiltration, ultrafiltration concentration and subpackaging to obtain a finished product. The compound has the advantages of high purity, high titer and good treatment, prevention and control effects.

The invention discloses a Fel D1 and Fel D4 fusion protein and application thereof, the Fel D1 and Fel D4 fusion protein is obtained in a prokaryotic expression mode, the fusion expressed protein greatly simplifies operation procedures in the aspects of inducing protein expression, purification, concentration, immunization and the like, and the economic cost is reduced. According to the preparation method of the egg yolk antibody provided by the invention, the egg yolk antibody obtained through fusion protein immunization laying hens can be subjected to antibody-antigen reaction with Fel D1 and Fel D4 respectively, and the titer of the egg yolk antibody is higher than that of a single antigen protein immunization group and a combined immunization group. The invention further provides a preparation method of the functional cat food, the egg yolk powder is added into the cat food according to a certain proportion, after the cat food is fed for a period of time, the content of Fel D1 and Fel D4 in cat hair and saliva is remarkably reduced, the cat food prepared from the fusion protein egg yolk antibody can resist Fel D1 and Fel D4 at the same time, and the blocking effect is remarkably improved.

The invention belongs to the technical field of bioactive peptides and antibodies, discloses an antigen epitope peptide of CaSR, a vaccine, an antibody and application, and aims to construct an egg yolk antibody targeting intestinal tract CaSR by using a polypeptide vaccine technology, improve the calcium absorption efficiency of poultry, seek a scheme for efficiently utilizing calcium elements of poultry, and realize calcium saving and emission reduction in production.

This application discloses a feline allergen Fel d1 egg yolk antibody and its preparation method and application, comprising: obtaining PCR amplification products through PCR amplification; constructing a recombinant plasmid by ligating the PCR amplification products with pET-28a(+); transforming the recombinant plasmid into Rosetta competent cells to obtain transformants; obtaining recombinant antigens by extracting proteins from the transformants; obtaining a subunit vaccine by mixing and emulsifying the recombinant antigens with white oil adjuvant; administering the subunit vaccine to laying hens to obtain immunized egg yolk liquid; adding Tris-HCl buffer and polyethylene glycol to the egg yolk liquid and centrifuging to collect the protein aqueous phase; filtering the protein aqueous phase to obtain a filtrate, adding polyethylene glycol, mixing, centrifuging to collect the precipitate; mixing the precipitate with PBS buffer, adding ammonium sulfate, and centrifuging to obtain the egg yolk antibody. This invention solves the problem that the safety and economy of existing feline allergy treatment methods cannot be simultaneously achieved.

The invention discloses riemerella anatipestifer and application of the riemerella anatipestifer in an egg yolk antibody. The riemerella anatipestifer is named as BRA22A011, and the preservation number of the riemerella anatipestifer is CGMCC (China General Microbiological Culture Collection Center) No.45887. The invention also discloses an inactivated vaccine, wherein the antigen in the inactivated vaccine contains the inactivated riemerella anatipestifer BRA22A011. The invention further discloses an egg yolk antibody, and the egg yolk antibody is prepared by using the riemerella anatipestifer BRA22A011 as an antigen. The use mode of the egg yolk antibody is intramuscular injection inoculation, and the injection dosage is 0.5 mL. The riemerella anatipestifer BRA22A011 has high pathogenicity and strong immunogenicity, and the egg yolk antibody prepared from the riemerella anatipestifer BRA22A011 can replace antibiotics and vaccines, is safe, free of residues, free of drug resistance and environment-friendly, and has a good prospect in clinical application of preventing and treating infectious serositis of ducks. The riemerella anatipestifer BRA22A011 and the Escherichia coli BE-20261 are adopted for preparing the egg yolk antibody together, and the prevention and treatment effects of the egg yolk antibody are more obvious.

The application provides a galactoside agglutinin 10 (Gal10) crystal and a preparation method and prepared antibody IgY and a pharmaceutical preparation thereof, and belongs to the technical field of medicines. The application uses the galactoside agglutinin 10 crystal as an antigen to prepare an egg yolk neutralizing antibody IgY capable of dissolving the Gal10 crystal, has the effects of improving rhinitis or asthma of a patient, preventing and treating rhinitis and asthma, and is safe, has no side effects, has a long-lasting curative effect, and has no drug resistance, thereby providing a safe, simple, hygienic, effective, economic and personalized treatment mode for prevention and treatment of rhinitis and asthma, and having a high market value.

The utility model relates to the technical field of egg yolk antibody extraction, and particularly discloses a separation and extraction device for antibodies in egg yolk, which comprises a treatment box, a bearing frame is fixedly mounted at the bottom end of the treatment box, a conveying pipe penetrates into one side of the top end of the treatment box, and a guide hopper is fixedly mounted at the lower part in the treatment box. A separation mechanism is arranged on one side of the conveying pipe at the top end of the treatment box; the separation mechanism comprises a motor, the motor is embedded in one side of the conveying pipe at the top end of the treatment box, a connecting plate is fixedly installed at the power output end of the motor, and a rotating cylinder is fixedly installed on the outer wall of the connecting plate. The communicating groove is communicated with the rotating cylinder, so that impurities such as residual residues and chippings can be discharged out of the rotating cylinder, and the situation that the quality of next separation and extraction is affected due to deterioration of the residual residues after a long time since the residues are difficult to clean due to adsorption is avoided.

The invention discloses an egg yolk globulin crude extraction method, extraction process and extraction equipment, and relates to the technical field of egg yolk globulin extraction.The process comprises the following steps that S1, egg yolk liquid is poured into a mold to be subjected to liquid nitrogen quick freezing, demolding is conducted, and the egg yolk liquid is smashed into particles; s2, mixing and stirring precooled acetone and the frozen egg yolk particles, centrifuging, and removing lipid precipitates on the upper layer; s3, adding the pre-cooled PBS, mixing, stirring and centrifuging, taking supernate, and carrying out treatment at the temperature of 0-5 DEG C; s4, slowly adding ammonium sulfate until the saturation degree is 30-40%, standing for 2 hours in an environment of 0-5 DEG C, then performing centrifugal treatment, taking precipitate, and redissolving the precipitate with PBS to obtain a redissolved solution; s5, performing ultrafiltration concentration on the reconstitution fluid by using an ultrafiltration membrane with the molecular weight cutoff of 100-200kDa; s6, carrying out ion exchange layer and hydrophobic layer chromatography treatment in sequence; and S7, carrying out pre-freezing in an environment of-80 DEG C, and then carrying out vacuum freeze-drying treatment. The method has the effect of improving the comprehensive benefit of extracting high-purity and high-activity egg yolk globulin.

The application relates to a yolk extraction device and a preparation method of egg yolk antibody. The extraction device comprises a workbench, a first linear module arranged on the workbench, a placing hole arranged on the first linear module, a pressing module arranged on the workbench and located above the first linear module, a lifting platform arranged on the workbench and located directly below the pressing module, an extraction shaft fixed at a first end of the lifting platform, a drill arranged on a second end of the extraction shaft and rotationally connected with the extraction shaft, a driving motor group arranged on the lifting platform, and two suction modules arranged on the lifting platform. The yolk extraction device and the preparation method of egg yolk antibody can directly extract egg white and egg yolk in the egg shell, so as to reduce the risk of contamination of the egg yolk liquid.

The invention relates to the technical field of biological product manufacturing, and particularly discloses an anti-shewanella-alginate egg yolk antibody as well as a preparation method and application thereof. The invention relates to an anti-shewanella algal egg yolk antibody, which is obtained by immunizing laying hens by using inactivated shewanella algal (RDSH-25070) thalli as antigens, collecting egg yolk liquid, separating and purifying, the shewanella algal (RDSH-25070) is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 14, 2025, is named as shewanella algal, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 14, 2025. The preservation number is CGMCC (China General Microbiological Culture Collection Center) The anti-shewanella-alginate egg yolk antibody disclosed by the invention has a strong inhibiting effect on shewanella alginate, can be effectively applied to preventing and treating diseases caused by the shewanella alginate for aquatic animals, and can be used for effectively retaining freshness and preventing corrosion of aquatic products at the same time.

Provided herein are methods of producing immunoglobulin Y (IgY) antibodies against monkeypox virus. The method includes immunizing hens with a peptide encoding at least one immunogenic domain of A29 fusion protein from the monkeypox virus and isolating the IgY antibodies against the A29 fusion protein from yolks of eggs laid by the immunized hens. Pharmaceutical compositions containing the IgY antibodies and methods of inhibiting or treating monkeypox virus infection are also provided.

The invention relates to the field of biological products, and particularly discloses a goose astrovirus, a goose astrovirus egg yolk antibody as well as a preparation method and application of the goose astrovirus egg yolk antibody. The invention relates to a goose astrovirus, which is named as RD240629A, is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No: 46197, and the preservation date is September 23, 2024. The goose astrovirus is named as RD240629A, and the goose astrovirus is named as RD240629A and is preserved in the China General Microbiological Culture Collection Center (CGMCC). The goose astrovirus can be used for preparing the egg yolk antibody for preventing gout of goslings, the egg yolk antibody is good in safety, no local or whole-body adverse reaction caused by antibody injection occurs, infection of the novel goose astrovirus can be effectively prevented and / or treated, and the anti-gout effect is good. The prepared egg yolk antibody is evaluated by adopting antibody titer determination and a challenge protection test, the titer of the egg yolk antibody is not lower than 1: 256, and the egg yolk antibody has a good commercial development prospect.

A composition and method for therapeutic or prophylactic treatment of mammals includes a mixture of IgY antibodies derived from one or more eggs laid by one or more avian species, wherein each of the one or more avian species have been vaccinated with one or more of the plurality of antigens. The mixture of IgY antibodies can be combined with a protective material that includes colostrum. In some embodiments the composition can also include IgA and IgM antibodies.

The invention discloses an indirect ELISA method for detecting cat allergen Fel d1 egg yolk antibody, comprising the following steps: coating cat allergen Fel d1 in the coating buffer with a coating buffer; blocking the non-specific antibody binding site of the cat allergen Fel d1 with a blocking solution; adding egg yolk antibody for incubation; adding HRP-labeled secondary antibody for incubation; adding a color developing solution for color development, and then adding a stop solution to stop the reaction; the amino acid sequence of cat allergen Fel d1 is shown in SEQ ID.NO.1. The method of the invention can effectively monitor the main cat allergen Fel d1, and provides a fast and simple tool for allergen screening, disease diagnosis and vaccine immune effect evaluation.

The present invention discloses an egg yolk antibody against Muscovy duck reovirus of goose origin, its preparation method and application, belonging to the technical field of veterinary biological products. The egg yolk antibody is prepared by immunizing poultry with a vaccine prepared using the inactivated Muscovy duck reovirus of goose origin with the preservation number CCTCC NO: V202491 as an antigen, and then extracting and purifying it from the egg yolk of highly immunized poultry eggs. Using the Muscovy duck reovirus of goose origin with the preservation number CCTCC NO: V202491 as a vaccine production strain to prepare an inactivated vaccine to immunize poultry can efficiently obtain the egg yolk antibody. This egg yolk antibody has good safety, high neutralization titer, and has a good prevention and treatment effect against the infection of Muscovy duck reovirus of goose origin, and has broad prospects for commercial development.

Provided herein are functionalized monoclonal antibodies (mAbs) including antibody fragments, including those where a Fab-binding molecule (Fab binding moiety) linked to a steric hindering molecule (steric hindering chemical moiety) is mechanically interlocked (e.g., through noncovalent conjugation) with the antibody or antibody fragment. Also provided are compositions that form highly stable and versatile drug delivery and diagnostic compositions.

The invention discloses a method for producing feed granules containing antiviral proteins for protecting aquatic animals from viral infestation by passive immunization. The particles contain an effective amount of an active antiviral protein that is incorporated into the basal feed raw material together with a stabilizer, a gelling agent and a bioavailability enhancer. The antiviral protein is an IgY antibody contained in a hyper-immune egg powder obtained from an egg produced from an avian species exposed to an antigen associated with viral infection. In order to protect shrimps from white spot syndrome virus (WSSV), the antigens consist of proteins made of active portions of the VP28 and VP19 sequences of the WSSV. In one embodiment, the stabilizer is sodium alginate, the gelling agent is carboxymethyl cellulose, and the bioavailability enhancer is piperine. In one embodiment, the active protein and related supplements are incorporated throughout the feed pellet, and in another embodiment, it is sprayed on the surface of the feed pellet.

Nanoparticles comprising a composition comprising a polypeptide having a molecular weight greater than 50,000 g / mol (e.g., IgY antibody), wherein the composition is encapsulated in a material comprising a biocompatible biodegradable polymer, are provided. Also provided are methods of preparing these nanoparticles and the use of the nanoparticles as a therapeutic agent for treating a disease state.

The present invention relates to a method of producing a binder-toxin fusion protein comprising at least, one protein binder selected from the group consisting of an antibody, an antibody fragment or derivative retaining target binding capacity, or an antibody mimetic, optionally, a peptide linker, and at least one protein toxin or protein protoxin. The method comprises the steps of: contacting a plant cell or a whole plant with a nucleic acid construct comprising in operational linkage at least the following (A) at least one polynucleotide encoding for the protein binder, or a target binding chain or domain thereof, and either B1) a polynucleotide encoding for a cleavable peptide linker and a polynucleotide encoding for a protein toxin, or B2) a polynucleotide encoding for a protein protoxin, which protoxin comprises a cleavable domain for activation thereof, allowing the construct to integrate into the nucleus of the plant cell, or of one or more cells of the whole plant, and expressing the fusion protein encoded by the nucleic acid construct (Fig. 7).

The invention provides a canine coronavirus strain as well as an inactivated vaccine and application thereof. The canine coronavirus strain is named as CCoV2015, and the genome full-length cDNA sequence of the canine coronavirus strain is as shown in SEQ ID NO. 1. The canine coronavirus strain is collected from intestinal tissues of naturally diseased dogs, sequence comparative analysis shows that the strain is a novel canine coronavirus strain, the infection ability of the strain is high, the strain is highly homologous with canine coronavirus strains separated in recent years, and the canine coronavirus strain is inactivated to prepare the canine coronavirus vaccine. Meanwhile, the canine coronavirus vaccine also fills the blank of a single canine coronavirus vaccine, and plays a very important role in prevention of canine coronavirus.

This disclosure provides, among other things, a transgenic chicken. In some embodiments, the transgenic chicken comprises B cells in which the endogenous immunoglobulin heavy chain locus comprises: (a) a functional immunoglobulin heavy chain gene comprising a nucleic acid encoding a heavy chain variable domain in which the CDR3 is in the range of 30-60 amino acids in length and comprises at least 2 cysteine residues; and (b) a plurality of pseudogenes that are operably linked to said functional immunoglobulin heavy chain gene and that donate, by gene conversion, nucleotide sequence to the nucleic acid encoding the heavy chain variable domain of (a), wherein the pseudogenes are upstream or downstream of the functional immunoglobulin heavy chain gene.

The invention discloses a preparation method and application of a composite antibody IgY for preventing and treating acne, and belongs to the technical field of cosmetics. The preparation method comprises the following steps: (1) preparing immunogens: respectively mixing inactivated propionibacterium acnes liquid, staphylococcus epidermidis liquid, staphylococcus aureus liquid and escherichia coli liquid with an ISA 78 adjuvant to obtain the immunogens of the four bacteria; (2) immunizing: respectively injecting immunogens of the four bacteria to laying hens, and collecting to obtain immunized eggs of the four bacteria; and (3) collection and preparation of a composite antibody IgY: mixing immunized eggs of the four bacteria, separating yolk, and carrying out acid treatment and impurity removal to obtain the composite antibody IgY. The prepared composite antibody IgY is high in titer, has the effect of preventing and treating acne and acne infection, is high in safety and remarkable in curative effect, and provides a new treatment strategy for preventing and treating acne.