37 results about "Chicken Infectious Anemia Virus" patented technology

The invention discloses a chicken infectious anemia virus LAMP (loop-mediated isothermal amplification) detection kit and a detection method thereof and relates to the technical field of biology. 198 highly conserved nucleotide sequences at the positions of 532bp to 729bp of a chicken infectious anemia virus gene sequence are selected as target regions to design a specific LAMP primer group; the specific LAMP primer group comprises positive and negative outer primers F3 and B3, positive and negative inner primers FIP and BIP and positive and negative ring primers LF and LB; and the nucleotide sequences are shown in SEQNO1-6. The high efficiency amplification of the target regions can be completed by the primer group in one hour; and agarose electrophoresis is dyed by a product according to the conditions, or the magnesium pyrophosphate precipitation turbidity of a by-product is detected, or after a color-developing agent is added, the color reaction is observed to judge a result. The kit formed by the invention has the advantages of convenience, rapidness, low price and strong specificity and is suitable for clinical diagnosis and monitoring of the chicken infectious anemia.

The invention relates to a method for rapidly testing chicken infectious anemia virus and a kit, which relate to the technical field of biology. The target region of chicken infectious anemia virus genes is amplified by using the specific LAMP primer group selectivity through the LAMP method to determine whether amplification products exist. The method of the invention reasonably avoids the inconvenience brought by the special requirements for temperature circulation of a conventional PCR; SYBR Green I stain can be added in the LAMP final product in the experiment for directly observing the results without the need of observation through gel electrophoresis or the turbidity monitoring method of the by-product-magnesium cell pyrophosphate. The method is simple and easy to be operated. A novel, rapid and easily-operated chicken infectious anemia virus identification and diagnosis method is provided by utilizing the method of the invention, further establishes the working foundation for controlling chicken infectious anemia, and has good economic benefits and market prospects.

The invention provides application of a perylenequinone compound in the preparation of feed for preventing and treating poultry viruses, and belongs to the technical field of biological engineering and agriculture. The invention relates to the preparation of the feed for preventing and treating the poultry viruses such as Marek's disease virus, avian leukosis virus subgroup J, chicken infectious anemia virus, avian reovirus, newcastle disease virus, infectious bursal disease virus, goose parvovirus, duck plague virus, avian influenza virus and the like by adding the perylenequinone compound into poultry feed. The perylenequinone compound comprises hypocrellin, elsinochrome, polyanticin, cladosporin, calphostin, cercosporin and the like, and has a series advantages of being convenient to extract, having good stability, having no obvious side effect for oral administration, realizing quick metabolism and the like. The dosage of the perylenequinone compound in the preparation of the feedfor preventing and treating the poultry viruses is 1-50umol of perylenequinone compound per kg by weight every day; the dosage of the perylenequinone compound in the preparation of the feed for preventing and treating the poultry viruses is 50umol-200umol of perylenequinone compound per kg by weight; moreover, the perylenequinone compound has very obvious effect of preventing and treating the poultry viruses, meanwhile can be produced through modern biotechnology, and can bring good economic and social benefits.

The invention discloses a multiple fluoroimmunoassay primer, kit and method for rapidly distinguishing 5 avian immunosuppression pathogens. Operation is simple, a target amplified fragment is acquired through a PCR, an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized, the MFI value is read through a detector, and viruses of different types are distinguished. The method can simultaneously detect the chicken infectious anemia virus, the chicken Marek's disease virus, the avian reticuloendotheliosis virus, the avian reovirus and the chicken infectious bursa disease virus accurately, specificity is high, sensitivity is high, and repeatability is good. Compared with a traditional detection method, the method achieves simultaneous detection of multiple different target molecules in the same sample, the use number of samples is small, operation is simple and rapid, and the detection cost can be greatly reduced.

The invention discloses a multiple fluorescence immunoassay primer, kit and method capable of fast distinguishing CAV, MDV, REV and IBDV. The multiple fluorescence immunoassay primer, kit and method has the advantages that target amplification fragments are obtained through PCR, \ amplification products, fluorescence encoded microspheres and streptavidin-phycoerythrin are hybridized, MFI values are read through a detector, and different types of pathogens are distinguished; the method can simultaneously and accurately detect chicken infectious anemia viruses, chicken Marek's disease viruses, chicken reticuloendotheliosis viruses and chicken infectious bursal disease viruses and is high in specificity, high in sensitivity and good in repeatability; compared with a traditional method, the method can simultaneously detect various molecules, with different purposes, in the same sample and is low in sample use amount, simple and fast to operate, capable of greatly lowering detecting cost, good in flexibility and capable of increasing and decreasing to-be-detected pathogen types on the basis.

The invention discloses a recombinant fowl pox virus live carrier vaccine expressing chicken infectious anemia virus VP1 and VP2 genes. The present invention constructs recombinant infectious clone pSY681-VP1-LacZ-VP2 on the basis of fowlpox virus transfer vector pSY681, and homologous recombination occurs between pSY681-VP1-LacZ-VP2 and FVP strain gene through homologous arms in CEF cells Get rFPV‑VP1‑VP2. The rFPV-VP1-VP2 virus strain can induce the body to produce effective antibodies against CAV, which plays an immune protection role; the offspring chickens obtain a high level of effective antibodies against CAV, which can well resist the invasion of CAV virulence, and play a role in immune protection. immune protection.