37 results about "Chicken Infectious Anemia Virus" patented technology

The invention provides a GeXP quick detection kit and detection method for simultaneously identifying 8 chicken infectious immunosuppression disease pathogens. The kit is used on the basis of a GeXP system, and comprises 8 pairs of PCR (polymerase chain reaction) primers. The detection result indicates that the kit has the advantages of high specificity and high sensitivity and can be used for simultaneously identifying and detecting chicken Marek's disease virus, A, B and J subgroup avian leucovirus, avian reticuloendothelium hyperplasia virus, avian reovirus, chicken infectious anemia virus and infectious bursal disease virus.

The invention discloses a multiple fluoroimmunoassay primer, kit and method for rapidly distinguishing 5 avian immunosuppression pathogens. Operation is simple, a target amplified fragment is acquired through a PCR, an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized, the MFI value is read through a detector, and viruses of different types are distinguished. The method can simultaneously detect the chicken infectious anemia virus, the chicken Marek's disease virus, the avian reticuloendotheliosis virus, the avian reovirus and the chicken infectious bursa disease virus accurately, specificity is high, sensitivity is high, and repeatability is good. Compared with a traditional detection method, the method achieves simultaneous detection of multiple different target molecules in the same sample, the use number of samples is small, operation is simple and rapid, and the detection cost can be greatly reduced.

The invention discloses a multiplex detection reagent for immunosuppressive poultry pathogens of avian influenza and the like and an application of the multiplex detection reagent. The multiplex detection reagent comprises six specific probes with sequences shown in Seq ID NO.1-Seq ID NO.6 which refer to AVI virus, ND (newcastle disease) virus, serotype I Marek's disease virus, IBDV (infectious bursal disease virus), avian infectious anaemia virus and ALV-J (aivan leukosis virus subgroup J) in sequence. The reagent comprises the specific probes for six immunosuppressive poultry pathogens comprising AVI and the like and can specifically capture target sequences of the six immunosuppressive poultry pathogens, and the foundation is laid for high-throughput rapid detection of the six immunosuppressive poultry pathogens. With adoption of the reagent, a rapid and accurate detection method is provided for the six immunosuppressive poultry pathogens on the basis of a liquid chip.

The invention relates to a method for rapidly testing chicken infectious anemia virus and a kit, which relate to the technical field of biology. The target region of chicken infectious anemia virus genes is amplified by using the specific LAMP primer group selectivity through the LAMP method to determine whether amplification products exist. The method of the invention reasonably avoids the inconvenience brought by the special requirements for temperature circulation of a conventional PCR; SYBR Green I stain can be added in the LAMP final product in the experiment for directly observing the results without the need of observation through gel electrophoresis or the turbidity monitoring method of the by-product-magnesium cell pyrophosphate. The method is simple and easy to be operated. A novel, rapid and easily-operated chicken infectious anemia virus identification and diagnosis method is provided by utilizing the method of the invention, further establishes the working foundation for controlling chicken infectious anemia, and has good economic benefits and market prospects.

The invention discloses an indirect ELISA (Enzyme-linked Immuno Sorbent Assay) kit for detecting a blood serum 8 type avian adenovirus antibody based on spike glycoprotein F. The indirect ELISA kit comprises an ELISA enzyme-linked plate which is coated with expression protein of blood serum 8 type avian adenovirus spike glycoprotein gene F, positive and negative controls, an HRP (Horseradish Peroxidase) labeled rabbit anti-chicken secondary antibody, a diluting solution, a color developing solution, a washing solution and a stopping solution. The ELISA kit disclosed by the invention can be used for specifically detecting a FAdV-8 antibody and does not react with anti-influenza virus blood serum, anti-Marek's virus blood serum, anti-Newcastle disease virus blood serum, anti-chicken infectious anemia virus blood serum, reticuloendotheliosis virus, ant-blood serum 4 type avian adenovirus blood serum, anti-egg drop syndrome virus blood serum and SFP chicken blood serum. Therefore, the kitdisclosed by the invention has good FAdV-8 specificity and can be used for FAdV-8 infection and immunization status epidemiological surveys.

The invention relates to an absolute fluorescent quantitative PCR detection method for chicken infectious anemia virus. Primers for specifically amplifying a VP1 gene segment of the chicken infectiousanemia virus are scientifically designed, and then the absolute fluorescent quantitative PCR method is established by using a TB Green<TM>Premix Ex Taq<TM>II reagent of the TaKaRa Company. Accordingto a highly conserved region of a VP1 gene of the chicken infectious anemia virus, upstream and downstream primers with the amplified fragment sizes of 140 bp are scientifically designed and synthesized, and the sequences of the primers are as follows: the sequence of the upstream primer is VP1-F: 5'-GCCCCGGTACGTATAGTGTG-3', and the sequence of the downstream primer is VP1-R: 5'-CCCGTACATGTGGTCTGCAT-3'. The method can be used for rapid and specific detection of chicken infectious anemia virus vaccine contamination residues and low-content chicken infectious anemia viruses in clinical samples by enterprises; and by quantitatively detecting the content of the chicken infectious anemia virus, the change rule and the like of the chicken infectious anemia virus in vivo can be known, and an effective means is provided for further researching the molecular biological characteristics of the chicken infectious anemia virus and making an effective prevention and control strategy.

The invention discloses a method for identifying avian leukosis virus and chicken infectious anemia virus through visual double LAMP. The invention provides a single-stranded DNA group for identifying avian leukosis virus and chicken infectious anemia virus through visual double LAMP. The single-stranded DNA group consists of single-stranded DNA molecules as shown in SEQ ID No.1-10. According to the invention, the visual double LAMP method for identifying and detecting the ALV and the CIAV is successfully established. The double LAMP can identify and diagnose the ALV and the CIAV in the same reaction tube, has the advantages of good specificity, high sensitivity, small pollution, convenience, rapidness and the like, a detection result can be directly observed by naked eyes, the result is judged according to the color of a reaction product, and the double LAMP is suitable for clinical rapid screening of the ALV and the CIAV.

The invention relates to application of a chicken anemia virus (CAV)-derived VP1-aa 23-43 polypeptide as an efficient cell penetrating peptide. The sequence of the VP1-aa 23-43 polypeptide is shown in SEQ ID NO.2. The polypeptide can carry FITC to enter a 293T cell, an HCT-116 cell, a 3T3 cell, an MDCK cell and an MSB1 cell within 30min; furthermore, the cell penetrating efficient is positively correlated to the concentration of the cell penetrating peptide; the key feature is that the polypeptide can efficiently enter suspension culture cells. The results of a laser scanning confocal microscope and a flow cytometry indicate that the cell penetrating efficiency of the CAV VP1-aa 23-43 polypeptide is over 2 times higher than that of a TAT oligopeptide at 10Mu M.

The invention relates to a use of a chicken anemia virus (CAV) VP1-aa 1-19 polypeptide as a highly efficient cell-penetrating peptide. The VP1-aa 1-19 polypeptide sequence is shown in SEQ ID NO. 2. The polypeptide can carry FITC and enter 293T cells, HCT-116 cells, 3T3 cells, MDCK cells and MSB1 cells in 30min, and the cell-penetrating efficiency is positively correlated with the concentration of the cell-penetrating peptide. The VP1-aa 1-19 polypeptide can efficiently enter suspension culture cells. The laser confocal microscope and flow cytometry result shows that the CAV VP1-aa 1-19 polypeptide has cell-penetrating efficiency significantly higher than that of a TAT short peptide.

The invention discloses multi-immunofluorescence assay primers, a kit and a method for detecting chicken Marek's disease viruses and chicken infectious anemia viruses. The primers, kit and method are simple in operation. The method comprises acquiring a target amplified fragment by PCR, then hybridizing the amplified product, fluorescence-coded microspheres and streptavidin-phycoerythrin, then reading an MFI value through a detector and distinguishing the different types of pathogens. The method can simultaneously detect the chicken Marek's disease viruses and chicken infectious anemia viruses, has the advantages of strong specificity, high sensitivity and good reproducibility, and can realize simultaneous detection of many different target molecules in the same sample. The multi-immunofluorescence assay primers have good flexibility and can detect more or less types of pathogens on the basis of the demands.

The invention discloses a colloidal gold test strip for detecting chicken infectious anemia virus. The colloidal gold test strip comprises a chicken anti-CIV (Huma Certain Immunodeficiency Virus) gold-labeled antibody glass fiber film, a nitrocellulose film, a water absorbing cushion and a PVC (polyvinyl chloride) bottom plate, wherein the nitrocellulose film is adhered to the center of the PVC bottom plate; the glass fiber film is formed by overlapping and connecting a sample cushion and a combining cushion; the other end of the combining cushion is adhered to one end with a T line, of the nitrocellulose film; the water absorbing cushion is adhered to one end with a C line, of the nitrocellulose film. With the adoption of the test strip for detecting, the sample is simply dropwise added onto the sample cushion of the test strip, and the operation is fast, simple and convenient, can be performed by one step, and is free of any instrument; the test strip is suitable for on-site use.

The invention provides a novel E-type aviadenovirus Fiber fluorescent quantitative PCR detection kit and an application thereof. A TaqMan probe fluorescent quantitative PCR (qPCR) detection method forE-type aviadenovirus AH720-8a is established, the amplification efficiency is 92%, and a target gene fragment can be effectively amplified. The repeatability and the accuracy are high. Clinical separation sample detection shows that the sensitivity of a method is 10 times higher than that of conventional PCR, and 29.3 copies/[mu] L;10<2> TCID50/mL the virus amount can be detected at least. The specificity is high, and detection results for other viruses infected with poultry, such as a Marek's disease virus Md5 virulent strain, a duck hepatitis virus type I (DHV-I), a duck Tembusu virus (DTMUV), a chicken infectious anemia virus, an avian enterovirus, FADV-4, chicken mycoplasma gallisepticum (MG),chicken mycoplasma synoviae (MS) and double distilled water, are negative. The establishmentof a diagnosis method can provide a technical means for rapid detection of the viruses, and the kit has important significance for promoting the healthy development of the poultry breeding industry.

The present invention discloses a primer set for identifying chicken parvovirus and chicken infectious anemia virus. The primer set comprises a primer pair I and a primer pair II. The primer pair I iscomposed of primers ChPV-F and ChPV-R, and the primer pair II is composed of primers CIAV-F and CIAV-R; and the primers ChPV-F, ChPV-R, CIAV-F, and CIAV-R separately have base sequences of SEQ.ID.NO.1 to SEQ.ID.NO.4 in the sequence table . On the basis, inventors also establish corresponding detection and identification methods and develop corresponding kits. An established duplex PCR can be usedfor rapid detection of mixed infection of the chicken parvovirus and chicken infectious anemia virus, is suitable for large-scale detection, can save costs and time, also reduces pollution and has very high practical value.

The invention provides application of a perylenequinone compound in the preparation of feed for preventing and treating poultry viruses, and belongs to the technical field of biological engineering and agriculture. The invention relates to the preparation of the feed for preventing and treating the poultry viruses such as Marek's disease virus, avian leukosis virus subgroup J, chicken infectious anemia virus, avian reovirus, newcastle disease virus, infectious bursal disease virus, goose parvovirus, duck plague virus, avian influenza virus and the like by adding the perylenequinone compound into poultry feed. The perylenequinone compound comprises hypocrellin, elsinochrome, polyanticin, cladosporin, calphostin, cercosporin and the like, and has a series advantages of being convenient to extract, having good stability, having no obvious side effect for oral administration, realizing quick metabolism and the like. The dosage of the perylenequinone compound in the preparation of the feedfor preventing and treating the poultry viruses is 1-50umol of perylenequinone compound per kg by weight every day; the dosage of the perylenequinone compound in the preparation of the feed for preventing and treating the poultry viruses is 50umol-200umol of perylenequinone compound per kg by weight; moreover, the perylenequinone compound has very obvious effect of preventing and treating the poultry viruses, meanwhile can be produced through modern biotechnology, and can bring good economic and social benefits.

The invention relates to the technical field of gene engineering, and discloses a chicken infectious anemia virus detection method based on a CRISPR/Cas12a system, which comprises the following steps: S1, treatment of tissue samples: weighing about 1g of each tissue sample, adding 1mL of PBS (Phosphate Buffer Solution), putting into a grinding bowl, grinding into homogenate, transferring into a sterile centrifugal tube, and centrifuging in a centrifugal machine at 8500rpm/min for 3 minutes; s2, virus nucleic acid extraction: extracting virus RNA by using a virus genome DNA/RNA extraction kit (centrifugal column type) of a certain biochemical technology Co., Ltd; a CRISPR/Cas12a system is combined with an ERA technology, the rapid molecular detection method for the chicken infectious anemia virus is established, the method has the advantages of visualization, high sensitivity, strong specificity, no dependence on expensive instruments and equipment and the like, the whole detection process only needs about 1 h, a new means is provided for rapid diagnosis of the chicken infectious anemia virus, and the method is suitable for large-scale popularization and application. And the method is of great significance to monitoring of immunosuppressive viruses.

The invention belongs to the field of virus detection, and particularly provides a triple TaqMan fluorescent quantitative PCR (Polymerase Chain Reaction) kit for simultaneously detecting three circoviruses, which integrates a forward primer, a reverse primer and a specific fluorescent probe of chicken infectious anemia virus (CAV). The kit comprises a forward primer, a reverse primer and a specific fluorescent probe of a circovirus avian type-1 (Gyrovirus galga1, GyG1), and also comprises a reverse primer and a specific fluorescent probe of the circovirus avian type-1 (GyG1). The kit comprises a forward primer, a reverse primer and a specific fluorescent probe of the circovirus human type 1 (Gyrovirus homsa1, GyH1), and further comprises a reverse primer and a specific fluorescent probe of the circovirus human type 1 (Gyrovirus homsa1, GyH1). The kit adopting the composition has the advantages of high sensitivity, high specificity, low pollution and real-time detection, and provides reliable technology and product for early diagnosis, monitoring, early warning, prevention and treatment of clinical circle virus diseases in livestock and poultry farms and animal food safety inspection.

The invention discloses a chicken infectious anemia virus genetic engineering vaccine. The chicken infectious anemia virus genetic engineering vaccine comprises fusion protein, the coding gene sequence of which is as shown in SEQ ID NO:1. The antigenicity, the immunogenicity and the function of the vaccine are similar to that of natural protein; the expression level is relatively high; the immunogenicity is high; the vaccine does not have pathogenicity to poultries, such as chickens; relatively high humoral immunity can be generated in the body of a poultry; an immunized poultry can resist strong toxicity and counteract toxicity; furthermore, the vaccine can be prepared by large-scale serum-free suspension culture through a bioreactor; and thus, the production cost of the vaccine is greatly reduced.

The invention discloses a primer group for identifying avian nephritis virus and chicken infectivity anemia virus. The primer group comprises a primer pair I and a primer pair II, wherein the primer pair I is composed of primers ANV-F and ANV-R, and the primer pair II is composed of primers CIAV-F and CIAV-R; the primers ANV-F, ANV-R, CIAV-F and CIAV-R have base sequences from SEQ. ID. NO.1 to SEQ. ID. NO.4 in the sequence table respectively. Therefore, the inventor also establishes a corresponding detection and identification method and develops a corresponding test kit. The duplex PCR established by the invention can be used for rapidly detecting mixed infection of avian nephritis virus and chicken infectivity anemia virus, is suitable for large-batch detection, can save cost and time, can reduce pollution, and has very high practical value.

The invention discloses a fluorescent quantitative PCR kit and primer for detecting a chicken infectious anemia virus. The primer comprises CIAV-124-F and CIAV-124-R, and is a primer pair capable of specifically amplifying a high-conservative gene segment in a VP1 gene of the CIAV, so that the anti-interference capability of detecting the CIAV by using molecular biology is effectively improved. The fluorescent quantitative PCR kit comprises the primer pair, tests prove that the lowest detection concentration of the fluorescent quantitative PCR kit is 2.0 * 10 <-1> copies/[mu]L, and the detection sensitivity of the fluorescent quantitative PCR kit is improved by 1000 times compared with that of common PCR. Moreover, the specificity is good, and an obvious amplification curve does not appear for common poultry diseases such as AIV-5, AIV-7, ILT, NDV, IBV and Fadv-4.