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Multiplex detection reagent for immunosuppressive poultry pathogens of AVI (avian influenza) and the like and application of multiplex detection reagent

An immunosuppressive, multiple detection technology, applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of cumbersome operation, long time, poor specificity, etc.

Active Publication Date: 2017-03-08
SHENZHEN CUSTOMS ANIMAL & PLANT INSPECTION & QUARANTINE TECH CENT +2
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AI Technical Summary

Problems solved by technology

[0009] Existing detection methods for immunosuppressive pathogens such as poultry avian influenza mainly include methods such as isolation and culture, electron microscopy, agar diffusion test, virus neutralization test, and enzyme-linked immunosorbent assay; but these detection methods are not time-consuming and cumbersome to operate , is the disadvantage of poor specificity

Method used

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  • Multiplex detection reagent for immunosuppressive poultry pathogens of AVI (avian influenza) and the like and application of multiplex detection reagent
  • Multiplex detection reagent for immunosuppressive poultry pathogens of AVI (avian influenza) and the like and application of multiplex detection reagent
  • Multiplex detection reagent for immunosuppressive poultry pathogens of AVI (avian influenza) and the like and application of multiplex detection reagent

Examples

Experimental program
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Embodiment

[0047] 1. Reagent consumables

[0048] 1. Strains and Chicken Embryos

[0049] The avian influenza virus H5N1 (Re-6) standard hemagglutination antigen and Newcastle disease standard hemagglutination antigen used in this example were purchased from Harbin Institute of Virology, 1 strain of Marek virus type 1, 1 strain of infectious bursal virus, chicken infectious bursal virus One strain of anemia virus and one strain of J subtype avian leukosis virus were preserved and provided by the Animal Inspection Laboratory of Shenzhen Entry-Exit Inspection and Quarantine Bureau.

[0050] In addition, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and other viruses that infect poultry as negative references, as well as bacteria that infect poultry such as Escherichia coli O157, Salmonella, and Listeria monocytogenes, were also tested. It is preserved and provided by the Animal Inspection Laboratory of Shenzhen Entry-Exit Inspection and Quarantin...

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Abstract

The invention discloses a multiplex detection reagent for immunosuppressive poultry pathogens of avian influenza and the like and an application of the multiplex detection reagent. The multiplex detection reagent comprises six specific probes with sequences shown in Seq ID NO.1-Seq ID NO.6 which refer to AVI virus, ND (newcastle disease) virus, serotype I Marek's disease virus, IBDV (infectious bursal disease virus), avian infectious anaemia virus and ALV-J (aivan leukosis virus subgroup J) in sequence. The reagent comprises the specific probes for six immunosuppressive poultry pathogens comprising AVI and the like and can specifically capture target sequences of the six immunosuppressive poultry pathogens, and the foundation is laid for high-throughput rapid detection of the six immunosuppressive poultry pathogens. With adoption of the reagent, a rapid and accurate detection method is provided for the six immunosuppressive poultry pathogens on the basis of a liquid chip.

Description

technical field [0001] The application relates to the field of detection of poultry immunosuppressive pathogens such as avian influenza, in particular to a reagent, detection method and application for multiple detection of poultry immunosuppressive pathogens such as avian influenza. Background technique [0002] There are many kinds of immunosuppressive pathogens in poultry, in addition to Marek's virus type 1 (MDV), infectious bursal virus, chicken infectious anemia virus, subtype J avian leukosis virus, Newcastle disease virus and avian influenza virus can also produce a strong immunosuppressive effect. [0003] Chicken Infectious Bursal Disease, also known as Gamboreau's disease, is an acute, contagious disease caused by Infectious Bursal Disease Virus (IBDV). It is characterized by inflammation, necrosis, atrophy, and severe damage of lymphocytes in the bursa of Fabricius. Thereby causing immune dysfunction in chickens and interfering with the immune effects of variou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/686C12Q1/701C12Q2600/16C12Q2537/143C12Q2521/107C12Q2531/113
Inventor 秦智锋孙洁陈兵卢体康马岚曾少灵刘建利郑晓聪吴绍强唐金明赵广武陈书琨林庆燕陶虹廖立珊詹爱军兰文升曹琛福王津津刘荭史秀杰贾鹏
Owner SHENZHEN CUSTOMS ANIMAL & PLANT INSPECTION & QUARANTINE TECH CENT
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