715results about How to "The detection method is accurate" patented technology

The image-pickup apparatus includes an image-pickup element including first type pixels photoelectrically converting an object image formed by a light flux from an optical system and second type pixels photoelectrically converting plural images formed by plural light fluxes divided from the light flux from the optical system, a controller performing contrast detection focus control using signals from pixels in a contrast evaluation area among the first type pixels, and a detector detecting phase differences of respective pairs of images of the plural images using signals from the second type pixels, the pairs of images being respectively formed in plural phase difference detection areas in the contrast evaluation area, and calculating information on an in-focus position of the optical system or object distance for each phase difference detection area based on each phase difference. The controller changes the contrast evaluation area based on the information calculated for the respective phase difference detection areas.

The invention discloses a method for using an electronic nose to detect the freshness of chicken. The invention aims at providing a method which uses the electronic nose to detect the freshness of chicken, has the advantages of rapidity and simpleness, easy use and objective and reliable detection result, and can reduce the impact of storing conditions to the detection result. The method comprises the following steps: a chicken sample to be detected is put into a sealed container and then heated in water bath with thermostatic control; the heated chicken sample is put into a test room, and then smell sent by the sample is detected by a gas sensor array which consists of four gas sensors of different types; the data which is aquired by the gas sensor array is collected and processed; and then the freshness of chicken is judged according to the processing result of the data. The detection method uses an electronic nose which consists of four gas sensors of different types to detect the chicken sample, and has the advantages of objective, accurate, and fast detection result, good repeatability, simple analysis process and small using amount of sensors, and reduces the extraction of eigenvalues.

The invention relates to a method for detecting a release situation of nicotine in buccal tobacco products, which is characterized by comprising the steps of simulating the release situation of the nicotine in the buccal tobacco products in the human mouth, instantly quantifying the release quantity and the release speed and then detecting the release speed and the release degree of the nicotine in the buccal tobacco products. The release and the collection of the nicotine are realized through a release and collection device in vitro; by means of the device, the release process of the nicotine in the buccal tobacco products in the human mouth can be simulated and nicotine release solutions can be the instantly collected, wherein the release process is a process that the nicotine is continuously extracted from the buccal tobacco products by utilizing artificial saliva at a constant temperature; and the detection of the nicotine content in the release solution is realized through a high performance liquid chromatography. The method has the advantages: the release speed and the release degree of the nicotine in various buccal tobacco products can be rapidly and accurately detected with good repeatability, high sensitivity and short detection period and is suitable for the rapid analysis of mass samples for different industries and different purposes.

The invention provides a method for the leak detection of an anesthesia machine system. The method comprises the following steps that: each gas passage of the anesthesia machine system, which is communicated with outside, is closed, and the anesthesia machine system becomes a closed containing cavity; an inspiratory valve is opened, the anesthesia machine system is inflated, and simultaneously, a pressure sensor of the gas passages is utilized to monitor the internal pressure of the anesthesia machine system at real time; when the internal pressure of the anesthesia machine system reaches a preset value, the inspiratory valve is closed, and inflation is stopped; the current internal pressure of the anesthesia machine system is detected; the internal pressure of the anesthesia machine system is detected again after a preset time; the decreased pressure difference of the internal pressure of the anesthesia machine system is calculated; and a corresponding data table of the pressure difference and the leak quantity is looked up according to the pressure difference, and the leak quantity leaked by the anesthesia machine system is calculated. The method can accurately detect the gas leak quantity leaked by the anesthesia machine system, and can enhance the moisture control and the monitoring accuracy of the anesthesia machine simultaneously.

The invention discloses a malicious application detection system for an android mobile phone, and belongs to the technical field of mobile phone detection. The malicious application detection system for the android mobile phone comprises a positive and negative sample collection module, a static feature extraction module, a dynamic feature extraction module, a neural network module and a monitoring result output module. According to the system, software code-based static analysis is combined with software behavior-based dynamic monitoring, so that a detection method is no longer static analysis purely based on a signature technology and judges whether software is malicious software or not by analyzing codes of the software and a behavior after running of the software; the detection methodis more accurate; and malicious software samples and secure software samples are collected to obtain static eigenvectors and a dynamic feature matrix, and an MLP neural network and an RNN are trainedthrough a large amount of samples to perform automatic learning and detection, so that the efficiency and the accuracy are greatly improved.

The present invention provides a SNP marker associated with pig growth rate, and application thereof, wherein the SNP marker is located on the 201 bp position of the pig LXR[alpha] gene exon 5, and the growth rate of the pig with the base A at the position is significantly higher than the growth rate of the pig with the base C at the position. According to the present invention, the polymerase chain reaction (PCR) and the sequencing technology are adopted to screen the SNP marker associated with pig growth rate, the restriction fragment length polymorphism (RFLP) method is adopted to detect the genotype of the pig gene to be detected, and the breeding of the predominant variety of the pig with the rapid growth is performed according to the genotype so as to accelerate the pig breeding process.

The present invention discloses a para-gene for an exogenous insertion vector of transgenic maize transformation event MON88017 and application thereof, the sequence of which is shown in table 1. The para-gene for the exogenous insertion vector of transgenic maize transformation event MON88017 provided by the invention can be used for specifically detecting whether the transgenic maize and related products contain transformation event MON88017 and the content of the transformation event; and the detection method is sensitive, accurate, simple and reliable, and has broad market prospects. The para-gene analyzes and confirms the sources of different bases in the para-sequence of the exogenous insertion vector of transgenic maize transformation event MON88017 for the first time, and determines the binding sites of the exogenous insertion vector sequence and the maize genome sequence, thus contributing to scientific research.

The invention provides an elisa kit for detecting cephalo-type medicine, containing: an ELIAS plate peridium of which is provided with peridium source, an enzyme label, cephalo-type medicine specific antibody working solution (contained when the peridium on the ELIAS plate is antigen and the enzyme label is enzyme label anti antibody or when the peridium on the ELIAS plate is anti antibody and theenzyme label is enzyme label antigen), ceftiofur standard solution, substrate developer, stop solution, concentrated cleaning solution and concentrated complex solution. The invention also disclosesa method applying the elisa kit for detecting cephalo-type medicine, including: firstly sample preprocessing is carried out, then the elisa kit is used for detection, and finally the detection result is analyzed. The elisa kit provided by the invention can be applied to detecting residual quantity of cephalo-type medicine in samples such as animal tissue (chicken, pork, fish and shrimp) and milk, etc, the operation is easy, the cost is low, the sensitivity is high, and the elisa kit is applied to on-site supervision and numerous sample screening.

The invention discloses a Cpf1 reagent kit for quickly detecting nucleic acid of an African swine fever virus. The Cpf1 reagent kit comprises a Cpf1 detection system suitable for quickly detecting theAfrican swine fever virus, and an immune colloidal gold test strip, wherein the Cpf1 detection system comprises specific crRNA protein, specific Cpf1 protein and a single-chain DNA(ssDNA) reporting system in accordance with a p72 gene of the African swine fever virus, the specific crRNA is one or more of crRNAs from ASFV P72 crRNA1 to ASFV P72 crRNA10, and the sequence of the specific crRNA is SEQ NO.4 to SEQ NO.13; and the single-chain DNA(ssDNA) reporting system comprises ssDNA FQreporter for fluorescence detection of a microplate reader and/or ssDNA DB reporter for detecting the immune colloidal gold test strip. According to the Cpf1 reagent kit disclosed by the invention, for the first time, the Cpf1 is used for detecting the African swine fever virus, and has the advantages of beinghigh in sensitivity, high in specificity, short in time consumption, high in flux, independent of large-scale experiment equipment and the like. The advantages enable a detection method based on the immune colloidal gold test strip developed by the invention to be conveniently used in basic laboratories and breeding enterprises to be used for performing detection, identification and diagnosis on basic quick detection of the African swine fever.

The invention belongs to the technical field of agricultural product quality analysis, and particularly relates to a near-infrared detection method for the peanut quality and application. The method comprises the following steps that peanut samples are collected, physical and chemical testing is performed on the peanut samples, near-infrared scanning is performed on the peanut samples, denoising processing and preprocessing are performed on obtained light absorption values, obtained preprocessed light absorption values are analyzed, near-infrared spectrum characteristic wavelengths are obtained through screening, and a prediction model of the peanut quality is built through a stepwise regression method. According to the near-infrared detection method for the peanut quality and the application, the obtained information is intuitive and reliable, the characteristic wavelengths of the peanut quality are determined and are few in number, and the analytical method that the model is built through the characteristic wavelengths is applied, so that the model precision is improved; on the condition of the same prediction precision, the prediction speed is high; meanwhile, through the built near-infrared prediction model method for the moisture, protein, fat, total sugar and ash content of peanuts, the peanut quality can be analyzed more comprehensively, and usage and popularization are easy.

The invention discloses a method for detecting a nicotine release behavior of a gum base type smoke-free tobacco product. The method for detecting the nicotine release behavior of the gum base type smoke-free tobacco product is characterized by being capable of simulating the release condition of nicotine in the gum base type tobacco product when the tobacco product is chewed in the mouth of a person and performing real-time and quantitative detection on a release amount and a release speed. The method comprises the specific steps of: (1) releasing and collecting nicotine in vitro; (2) performing quantitative analysis on the content of the nicotine in collected liquid and residual samples; and (3) drawing an in-vitro nicotine release curve. The method can be used for detecting the nicotine release speed and the nicotine release degree of the gum base type tobacco product so as to provide the technical support for researching and judging the safety of the gum base type smoke-free tobacco product and performing quality regulation and control. The method is close to the true condition of the gum base type smoke-free tobacco product consumed by the person, is rapid and exact, good in repeatability and short in detection period, is suitable for detection of the gum base type smoke-free tobacco product in various sizes and is convenient to popularize.

The invention provides an enzyme-linked immunosorbent kit for inspecting sulfa drugs, comprising an ELISA plate which is coated with coating antigen, an enzyme label, sulfa drug specific antibody working liquid (being contained when the antigen is coated on the ELISA plate and the enzyme label is enzyme labeling antibody or antibody is coated on the ELISA plate and the enzyme label is enzyme labeling antigen), sulfamethoxy-isoxazole standard product solution, substrate color development solution, stop solution, concentrated washing liquid and concentrated complex solution. The invention further discloses a method which applies the enzyme-linked immunosorbent kit for inspecting the sulfa drugs, and the method comprises the steps of firstly carrying out the pre-treatment on a sample, then using the kit for inspecting and finally analyzing the inspection result. The provided enzyme-linked immunosorbent kit can be used for inspecting the residual amount of the sulfa drugs in animal tissues(chicken, pork, fish and shrimp), honey, eggs, milk, feeds and other samples, the operation is simple, the cost is low, the sensitivity is high and the enzyme-linked immunosorbent kit can be monitore d on-site and is applicable in screening mass samples.

The invention relates to a method for detecting pre-treatment of nitrofurans metabolite, comprising the following steps: (1) choosing a sample; (2) uniformly mixing the sample and extractant to obtain supernate; (3) adding the extractant to carry out extraction once again and obtaining mixed extractant; (4) adding derivatization reagent and catalyst to the mixed extractant and uniformly mixing the mixture; (5) cooling the mixture to room temperature; (6) adjusting the mixed extractant to neutrality and adding ethyl acetate to carry out extraction; (7) repeating the operation and combining ethyl acetate layers; (8) carrying out rotary evaporation on the mixture to be dry and adding methanol solution to dissolve residue; (9) activating a purification pillar to cause the methanol solution to pass through the purification pillar and obtaining and drying eluent; (10) adding acetonitrile aqueous solution and isooctane to the residue, dissolving and obtaining lower acetonitrile water layer; (11) causing the acetonitrile water layer to pass through a microfiltration membrane; and (12) analyzing and measuring the components and contents of the nitrofurans metabolite. The method of the invention is accurate, reliable and highly sensitive and enjoys good technical effect.

Composition including an oleophilic base ink composition having dispersed therein particles of an infrared-absorbing material wherein the infrared-absorbing material is selected from reduced indium tin oxide, titanium nitride, zirconium nitride, molybdenum metal, copper chromate, lanthanum hexaboride, neodymium oxide, neodymium chloride, dysprosium oxide and praseodymium oxide.

The invention belongs to the technical fields of crop nutrition diagnosis and fertilization, and in particular relates to a fertilizing method based on nitrogen nutrition nondestructive detection of winter rapes. The fertilizing method is characterized by comprising the following steps: testing a SPAD value by selecting the key growing period and time of the rapes, determining the sensitive leaf position and leaf part subjected to the nitrogen nutrition detection, establishing a critical leaf SPAD value detection method, carrying out real-time nitrogenous fertilizer topdressing by virtue of obtained detection values, and the like. The fertilizing method disclosed by the invention can be used for evaluating and detecting the nitrogen nutrition sufficiency and deficiency level of the rapes relatively accurately; and compared with a conventional laboratory chemical analysis method, the method, as a nondestructive rape nitrogen nutrition status detection and fertilization method, is real-time and rapid.

The invention discloses a composite affinity column for purifying 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone, a preparation method and an application thereof. According to the invention, by employing 4% of cylindrical agarose gel as a solid phase carrier, agarose gel and an antibody are coupled to form an immunoadsorbent, and filling in a column to prepare the immunization affinity column. When a sample containing 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone passes through the immunization affinity column, 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone can be specifically adsorbed by the immunoadsorbent, other impurities flows out of the immunization affinity column, then methanol is used for eluting 3-acetyl deoxynivalenol, aflatoxin, ochratoxin A and zearalenone from the column, so that the sample can be better purified.

A gene chip for detecting the pathogenic bacteria from blood features that the specific probes, derivative probes, complementary probes, and universal probes are arranged on the nylon film to detect different pathogenic bacteria. Its advantages are high speed and high correctness.

The invention provides an enzyme linked immune regent box to detect neomycin medicine. It includes: the enzyme labeled board which encrusts the encrusting substance, the enzyme marker, the special antibody for the neomycin, the neomycin standard solution, the substrate color solution, the stop solution, the condense washing solution, the condense recovery solution. The invention also discloses the method which includes the process: first to pre-treat the sample, then to detect by the regent box and last to analyze the detecting result. The invention is to detect the neomycin residue weight in the animal food such as the chicken, chicken liver, pork, pork liver, milk powder, egg and feedstuff. The method is simple, low cost and has high sensitivity, also it is proper for detect the mass sample.