23results about How to "The detection method is accurate" patented technology

The invention discloses a method for determining dextrocarnitine in a levocarnitine product by using high performance liquid chromatography, and the chromatographic conditions are as follows: cellulose-tri-(3, 5-dimethyl phenyl carbamate) is used as a filler for a chromatographic column, a hexafluorophosphate buffer solution-acetonitrile is used as a mobile phase, the volume ratio is 77: 23-69: 31, the column temperature is 15-25 DEG C, the flow velocity is 0.3-0.5 ml / min, and the detection wavelength is 246nm. The method disclosed by the invention can be used for rapidly and accurately detecting the dextrocarnitine in the levocarnitine product and calculating the content of the dextrocarnitine, is simple to operate and good in reproducibility, can better realize quality control of the levocarnitine preparation and an intermediate product thereof, and provides guarantee for synthesis and preparation process optimization.

The invention discloses a hapten for detecting the content of isofenphos-methyl and application of the hapten, particularly relates to the hapten for detecting the content of isofenphos-methyl and a preparation method of the hapten, and further discloses application of the hapten in the aspects of antibodies and kits. The enzyme linked immunosorbent assay kit established by aiming at the hapten for detecting the content of isofenphos-methyl can realize rapid detection of a large batch of samples to be detected, is convenient to use and low in detection cost, the detection method is efficient, accurate and rapid, can simultaneously detect a large batch of samples, and is suitable for on-site monitoring of isofenphos-methyl in fruits, vegetables and traditional Chinese medicinal materials and screening of a large number of samples.

The present invention discloses a method for detecting pathogens by combining a SERS-based CRISPR / Cas system with a microfluidic paper analysis device and its application. The method only requires extracting the genome of the pathogen in a sample, and after RPA isothermal amplification, the CRISPR / Cas system can be used to react with a sandwich gold nanostar probe on a μPAD to achieve detection of the microbial pathogen Salmonella. The detection range is wide, the method is simple, fast and easy to operate.

The embodiment of the invention discloses a contactor contact adhesion detection method and device, electronic equipment and a storage medium. The method comprises the steps that a contactor is controlled to be conducted, and the first temperature of a heater is obtained; controlling the contactor to be disconnected to obtain the current second temperature of the heater; after the contactor is disconnected, a fan for cooling the heater is controlled to run for a first preset time, and a third temperature of the heater when the fan stops running is obtained; after the fan runs for the first preset time, the heater is controlled to stand for the second preset time, and the fourth temperature of the heater after standing is obtained; and determining whether the contactor contact is adhered according to the difference degree of the second temperature, the third temperature and the fourth temperature. According to the technical scheme provided by the embodiment of the invention, an auxiliary detection contact does not need to be additionally arranged, whether the contact of the high-voltage direct-current contactor is adhered or not can be judged only by comparing the temperatures of the high-voltage PTC heater in different states, and the detection method is simple, accurate and relatively low in cost.

The invention belongs to the technical field of pharmaceutical analysis, particularly relates to a method for detecting methylamine in Vonoprasone fumarate, and provides a convenient, efficient and accurate detection method for solving the problem of detection of methylamine in Vonoprasone fumarate. Derivative products are detected by liquid chromatography, the content of methylamine in the Vonoprazan fumarate can be detected, so that medication safety is effectively guaranteed, quality control of the Vonoprazan fumarate is facilitated, the method is convenient, efficient and accurate, the system applicability, repeatability, specificity and accuracy completely conform to the guidance principle verified by methods in Chinese pharmacopoeia, and the method is suitable for popularization and application. The method can be used for quality control of the Vonoprasone fumarate.

The present invention relates to the field of molecular biology detection technology, and in particular to a primer combination, a kit and a method for detecting human neutrophil antigen genotyping. The present invention designs a forward primer, a reverse primer and an extension primer for all 11 SNP sites of the human granulocyte antigen HNA 1-5 system, and the sequence is shown in SEQ ID No: 1-22. The method for detecting human neutrophil antigen genotyping using the primer combination can achieve batch detection of 96 or 384 samples per batch, and high-throughput detection will bring great convenience to related clinical diagnosis and scientific research work, greatly save experimental time, and the detection method is accurate and stable.

The invention discloses a method for determining related substances of cis-tetrahydrocarboline hydrochloride, an intermediate of tadalafil, which specifically adopts a diode array detector and uses 0.1% formic acid solution-acetonitrile as a mobile phase for gradient elution. Take an appropriate amount of cis-tetrahydrocarboline hydrochloride, add a solvent (water: acetonitrile 1:1) to prepare a solution containing 0.4 mg of cis-tetrahydrocarboline hydrochloride per 1 ml, as a test solution. Take the test solution and inject it into a liquid chromatograph, record the chromatogram, and process the chromatogram according to the area normalization method. The detection method of related substances of cis-tetrahydrocarboline hydrochloride, an intermediate of tadalafil, can quickly and accurately detect the situation of related substances of cis-tetrahydrocarboline hydrochloride. The operation is simple and the sensitivity is high, and the product quality can be well controlled.

The utility model discloses a steering system connecting shaft flat square anti-secondary processing detection device, which comprises a matching mechanism, a guide sleeve and a reminding mechanism, and is characterized in that the matching mechanism comprises a base and a detection seat arranged at the top of the base, and the guide sleeve is arranged at the top of the detection seat; according to the utility model, displacement detection is carried out on the flat square of the connecting shaft through the displacement sensor, the detection end of the displacement sensor is located at the bottommost layer of the flat square channel, when the flat square passes, a displacement signal is detected, and the warning lamp is electrically connected with the displacement sensor. When the flat square does not pass through the flat square channel and cannot reach the bottommost layer to be detected by the detection end, the indicating lamp does not emit light, the flat square is large in size, the connecting shaft is unqualified, secondary processing is needed, and the detection method is simple and more accurate.

The invention belongs to the technical field of biopharmacy analysis, and provides a # imgabs0 # 13-S9 content detection method, which comprises the operation of detecting a sample to be detected by adopting an RP-UPLC-ELSD (Reverse P-Ultra Performance Liquid Chromatography-Evaporative Light Scattering Detector) method, mobile phases adopted in the RP-UPLC-ELSD method comprise a mobile phase A and a mobile phase B; wherein the mobile phase A is water, and the mobile phase B is methanol; according to the method, the mobile phase is adopted for gradient elution. According to the detection method for the content of the # imgabs1 # 13-S9, provided by the invention, the residual quantity of the # imgabs2 # 13-S9 in the recombinant protein vaccine stock solution can be accurately determined, and the detection method is rapid, accurate, linear and good in specificity, is not interfered by other components in the recombinant protein vaccine stock solution, and has guiding significance for detecting the residual quantity of the # imgabs3 # 13-S9 in a histone vaccine stock solution.

The invention provides a method for detecting impurities in (3R, 4R)-1-benzyl-N, 4-dimethylpiperidine-3-amine dihydrochloride, which comprises the following steps: (1) mixing a reference substance with a blank solution to obtain a reference substance solution; (2) mixing a to-be-detected sample with a blank solution to obtain a test solution; and (3) respectively carrying out sample introduction on the test solution, the reference solution and the blank solution, and carrying out liquid chromatography-tandem mass spectrometry detection to obtain a detection result. The detection method provided by the invention can be used for simultaneously detecting four mutagenic impurities in (3R, 4R)-1-benzyl-N, 4-dimethylpiperidine-3-amine dihydrochloride, and is accurate, high in precision and good in repeatability.

The application belongs to the technical field of pharmaceutical analysis, and particularly relates to a detection method of ammonia in methamphetamine, which is a convenient, efficient and accurate detection method for solving the problem of ammonia detection in methamphetamine. The method uses gas chromatography to detect the content of ammonia in methamphetamine, has low detection cost, and is convenient for quality control of methamphetamine. The method is convenient, efficient and accurate, and fully meets the guiding principles of method verification of Chinese Pharmacopoeia in terms of system applicability, repeatability, specificity and accuracy, and can be used for quality control of methamphetamine.

The invention relates to a KASP primer group for identifying a molecular marker of a wheat low-gluten gene LGP1, a kit and application, and in particular, a gene sequence of the wheat low-gluten gene LGP1 is obtained by cloning in common wheat, and a KASP primer Kasp-lgp1-C / T / R is designed according to the difference of different sequences. Experiments prove that the molecular marker is accurate, reliable and good in repeatability, can be used for screening the wheat low-gluten gene LGP1, provides a simple and accurate detection method for effective identification and selection of the wheat low-gluten gene LGP1 in the wheat quality improvement process, and has important significance on wheat quality improvement.

The invention belongs to the technical field of pharmaceutical analysis, particularly relates to a method for detecting 4-(1, 1-dibromoethyl)-6-chloro-5-fluoropyrimidine in voriconazole, and provides a convenient, efficient and accurate detection method for solving the problem of detection of 4-(1, 1-dibromoethyl)-6-chloro-5-fluoropyrimidine in voriconazole. The content of 4-(1, 1-dibromoethyl)-6-chloro-5-fluoropyrimidine in voriconazole can be detected, so that the medication safety is effectively guaranteed, the quality control of voriconazole is facilitated, the method is convenient, efficient and accurate, the system applicability, repeatability, specificity and accuracy completely conform to the guidance principle verified by methods in Chinese Pharmacopoeia, and the method is suitable for popularization and application. The method can be used for quality control of voriconazole.

The invention discloses a molecular marker in a bovine KCNIP1 gene and application of the molecular marker. The molecular marker is an SNP (Single Nucleotide Polymorphism) site g.2487025Ggt in a bovine genome; a. The molecular marker or the substance for detecting the single nucleotide polymorphism of the cattle molecular marker provided by the invention can be applied to cattle genetic breeding, so that the number of red blood cells and the hemoglobin concentration of improved cattle offspring in Tibetan are reduced, and the problem of hypoxia faced by cattle breeding in a plateau environment is solved.

The utility model belongs to the technical field of direct-current charging, and discloses a fault detection device of an output relay of a direct-current charging system, which is characterized in that a pin 3 of an operational amplifier U1 is connected with one end of a resistor R1 and one end of a resistor R3, the other end of the resistor R1 is connected with a port c, the other end of the resistor R3 is connected with a port GND, a pin 2 of the operational amplifier U1 is connected with one end of a resistor R2 and one end of a resistor R15, and the other end of the resistor R2 is connected with the port GND. The other end of the resistor R2 is connected with the port d, the pin 1 of the operational amplifier U1 is connected with one end of the resistor R7 and the other end of the resistor R15, the other end of the resistor R7 is connected with one end of the capacitor C2 and the port UCD, and the other end of the capacitor C2 is connected with the port GND. The beneficial effects of the utility model are that the fault state of the relay is judged by detecting the voltages at the front end and the rear end of the main contact of the output relay and is judged by combining the state of the auxiliary contact of the output relay, thereby improving the reliability.

The present application provides a molecular marker in the ADAM18 gene of cattle and its application. The molecular marker is one or more of g.34873341T>A, g.34874222C>T and g.34877973C>G in the cattle genome. The molecular marker or the material used to detect the single nucleotide polymorphism of the molecular marker of cattle provided by the present application can be used in cattle genetic breeding to reduce the hemoglobin concentration of the offspring of Tibetan improved cattle and solve the low oxygen bottleneck problem faced by the breeding of plateau cattle.

The invention discloses a method for detecting food-borne pathogenic bacteria based on Argonaute protein in combination with loop-mediated isothermal amplification and electrochemical biosensing, and belongs to the technical field of microbiological detection and biosensing. The integrated detection method based on Argonaute protein, loop-mediated isothermal amplification and electrochemical biosensing, which is high in sensitivity, strong in specificity, simple and convenient to operate and suitable for on-site rapid detection, is provided aiming at the problems that an existing food-borne pathogenic bacterium detection method is insufficient in sensitivity, depends on complex instruments, is difficult to apply on site and the like. Target nucleic acid is subjected to exponential amplification through LAMP (loop-mediated isothermal amplification), then Argonaute-mediated two-stage specific recognition and signal transduction are performed, and finally reading is performed through a high-sensitivity electrochemical platform, so that the detection limit of salmonella typhimurium as high as 1 CFU / mL is realized, and the detection range can cover 100-108 CFU / mL.

The invention discloses an image color difference detection method and system for industrial production. The method comprises: converting a pure color image to be detected from an RGB color space to an HSV color space and a CIELAB color space respectively to obtain a first pure color image to be detected and a second pure color image to be detected; extracting hue, saturation and brightness information in the first pure color image to be detected, calculating the color difference of each pixel, and marking an area where the color difference is greater than a first adaptive threshold as a first area; performing Gaussian filtering optimization on the first area in the second pure color image to be detected, extracting L, a, and b components, calculating the color difference of each pixel to obtain an optimized mixed color detection result, comparing the optimized mixed color detection result with a second adaptive threshold, and marking the mixed color area. The invention realizes efficient and accurate pure color image mixed color detection through multi-color space conversion and adaptive threshold calculation, and has broad application prospects.

This invention provides a method for detecting the diethyl sulfate content in methyl 4-acetamido-2-ethoxybenzoate. The method involves derivatizing the sample with sodium iodide-ascorbic acid and detecting the sample using a headspace gas chromatograph equipped with an ECD detector. The chromatographic column is an Agilent DB-624 30m × 0.53mm × 3μm column, the injection port temperature is 220℃, the detector temperature is 250℃, and the carrier gas is N2. 2， Flow rate: 1.5 mL / min, temperature programmed: initial column temperature 70℃, increased to 150℃ at a rate of 5℃ / min and held for 0 min; then run at 240℃ and held for 5 min, flow rate 3 mL / min. This invention provides a convenient, efficient, and accurate method for detecting the diethyl sulfate content in methyl 4-acetamido-2-ethoxybenzoate. This method uses gas chromatography to detect the diethyl sulfate content in methyl 4-acetamido-2-ethoxybenzoate, thereby effectively ensuring medication safety.

The invention discloses a method and a kit for detecting sodium cyclamate in baijiu, and belongs to the technical field of food safety detection. The hapten is obtained, the sodium cyclamate monoclonal antibody is prepared according to the hapten, the enzyme linked immunosorbent assay kit is further prepared through specific binding of the sodium cyclamate monoclonal antibody and sodium cyclamate, and qualitative and quantitative detection can be conducted on sodium cyclamate in baijiu. The detection method is rapid, efficient and high in sensitivity, the lowest detection limit of sodium cyclamate in various types of baijiu is as low as 2.75-2.82 mu g / L, the detection variable coefficient RSD is smaller than 5%, the detection result is accurate and sensitive, and the problems that an existing detection method is complex in sample pretreatment step, high in instrument and equipment condition requirement and the like are effectively solved.

The present invention discloses a hapten, an artificial antigen, an antibody and a preparation method and application thereof. The structural formula of the hapten is shown in formula (I), and the hapten is used to prepare artificial antigens and antibodies for detecting lipopolysaccharide saponins. The enzyme-linked immunosorbent assay kit prepared by using the artificial antigen and antibody for lipopolysaccharide saponins provided by the present invention has a detection sensitivity of 0.0103 ng / mL for lipopolysaccharide saponins, and IC 50 The linear range is 0.01-10 ng / mL. The enzyme-linked immunosorbent assay kit is easy to use, has low detection cost, and is fast, efficient, and accurate. It is suitable for the quality control of sea cucumber saponins in sea cucumber tissues or the on-site rapid detection of sea cucumber saponin residues and the screening of a large number of samples, and has good application prospects.

The invention discloses a method for determining the content of a traditional Chinese medicine composition containing angelica sinensis and cinnamon, which comprises the following steps: taking a test solution and a reference solution of the traditional Chinese medicine composition, and detecting under the chromatographic conditions that a chromatographic column with octadecyl bonded silica gel as a filler is adopted; the mobile phase A is selected from one or more of acetonitrile, methanol and tetrahydrofuran, and the mobile phase B is an acid aqueous solution, an alkali aqueous solution and / or a buffer salt aqueous solution; obtaining content information of a reference substance of the traditional Chinese medicine composition according to a detection result; wherein the traditional Chinese medicine composition comprises angelica sinensis, cinnamon, radix paeoniae alba, ginger, Chinese dates and liquorice, and the reference substances are ferulic acid, cinnamaldehyde, 6-gingerol and ligustilide. The detection method is simple, accurate, efficient, good in stability, high in precision, good in reproducibility, convenient and easy to master, energy can be effectively saved, the analysis cost is reduced, and the technical content is not disclosed in the prior art.