3results about How to "The detection method is accurate" patented technology

The application belongs to the technical field of pharmaceutical analysis, and particularly relates to a detection method of ammonia in methamphetamine, which is a convenient, efficient and accurate detection method for solving the problem of ammonia detection in methamphetamine. The method uses gas chromatography to detect the content of ammonia in methamphetamine, has low detection cost, and is convenient for quality control of methamphetamine. The method is convenient, efficient and accurate, and fully meets the guiding principles of method verification of Chinese Pharmacopoeia in terms of system applicability, repeatability, specificity and accuracy, and can be used for quality control of methamphetamine.

The invention discloses a method for detecting food-borne pathogenic bacteria based on Argonaute protein in combination with loop-mediated isothermal amplification and electrochemical biosensing, and belongs to the technical field of microbiological detection and biosensing. The integrated detection method based on Argonaute protein, loop-mediated isothermal amplification and electrochemical biosensing, which is high in sensitivity, strong in specificity, simple and convenient to operate and suitable for on-site rapid detection, is provided aiming at the problems that an existing food-borne pathogenic bacterium detection method is insufficient in sensitivity, depends on complex instruments, is difficult to apply on site and the like. Target nucleic acid is subjected to exponential amplification through LAMP (loop-mediated isothermal amplification), then Argonaute-mediated two-stage specific recognition and signal transduction are performed, and finally reading is performed through a high-sensitivity electrochemical platform, so that the detection limit of salmonella typhimurium as high as 1 CFU / mL is realized, and the detection range can cover 100-108 CFU / mL.

This invention provides a method for detecting the diethyl sulfate content in methyl 4-acetamido-2-ethoxybenzoate. The method involves derivatizing the sample with sodium iodide-ascorbic acid and detecting the sample using a headspace gas chromatograph equipped with an ECD detector. The chromatographic column is an Agilent DB-624 30m × 0.53mm × 3μm column, the injection port temperature is 220℃, the detector temperature is 250℃, and the carrier gas is N2. 2， Flow rate: 1.5 mL / min, temperature programmed: initial column temperature 70℃, increased to 150℃ at a rate of 5℃ / min and held for 0 min; then run at 240℃ and held for 5 min, flow rate 3 mL / min. This invention provides a convenient, efficient, and accurate method for detecting the diethyl sulfate content in methyl 4-acetamido-2-ethoxybenzoate. This method uses gas chromatography to detect the diethyl sulfate content in methyl 4-acetamido-2-ethoxybenzoate, thereby effectively ensuring medication safety.