63 results about "Peridium" patented technology

The invention discloses double-antibody sandwich ELISA (enzyme linked immunosorbent assay) kit used for detecting an avian leukosis group specific antigen. The kit comprises an enzyme plate coated by a monoclonal antibody which is secreted by a hybridoma cell strain the preservation serial number of which is CGMCC (china general microbiological culture collection center) No.5961. The invention also discloses a double-antibody sandwich ELISA method which is established by utilizing the monoclonal antibody and is capable of rapidly and effectively detecting an ALV (avian leukosis virus). In the double-antibody sandwich ELISA method, the monoclonal antibody prepared by pronucleus expressive HLJ09mdj-1p27 albumen is utilized as a peridium antibody, and antibodies prepared by p27 are utilized as a detection antibody. According to the invention, the minimum detection amount of the p27 is 1.25 ng/ml, the method is not reacted with the common virus of birds, and the specificity is good. The method is utilized to detect egg white and an anus swab sample, and the coincidence rate is respectively 96.5% and 88.9% compared with a PCR (polymerase chain reaction) method; and the result proves that the method has the advantages of convenience, celerity, differentia, sensitivity and the like, and is useful for the detection and population purification of the ALV.

The invention relates to a PCV2 (porcine circovirus 2) ELISA (enzyme-linked immuno sorbent assay) antigen detection kit as well as a preparation method and applications thereof, wherein the detection kit comprises an elisa plate of a polyclonal antibody of peridium anti-PCV2-Cap (nucleocapsid) protein, seal liquids, sample diluent, an antigen standard product, a second antibody of a monoclonal antibody of HRP marked anti-PCV2-Cap protein, a concentrated washing liquid, an enzyme substrate solution A, an enzyme substrate solution B and a stop solution, wherein the antigen standard product is purified reconstructed PCV2-Cap protein. The specificity of the kit provided by the invention achieves 100%, and the sensitivity is as high as 4ng/ml, and the kit can be used for swinery PCV2 antigen detection and PCV2 vaccine product quantitative detection.

The invention discloses the production method and use for imidacloprid artificial hapten, artificial antigen and specific antibody, wherein the production method comprises, using imidacloprid (1-(6-chlorine-3-picolyl)-N-nitro-2-imidazoline imine) as raw material for reaction with 3-mercaptopropionic acid under alkaline condition, thus synthesizing hapten 1-(6-(2-carboxyethyl) sulfo-3-picolyl)-N-nitro-2-imidazoline imines (IM), then coupling with proteins through carbodiimide method and mixed anhydride method to prepare artificial antigens (immunogens and peridium antigens).

The invention relates to a diagnostic reagent kit for testing the hepatitis c virus (HCV) and the preparation and test method, which is to add the HCV recombinant antigen used for peridium into the buffer solution, blend it, move into the luminous microplate, make incubation for 18 hours under 4DEG.C, wash the luminous microplate, add into the confining liquid, leave the liquid after incubation and fully dry the luminous microplate to complete the preparation of the pre-peridium luminous microplate; combine the anti-human IgG used for marking and the horse radish peroxidase by improving the sodium periodate to complete the preparation of the enzyme marker; prepare the chemical luminous substrate solution A with luminal, Tween20 and luminous intensifier and prepare the chemical luminous substrate solution B with the hydrogen peroxide. The reagent kit also comprises the sample diluent and concentrated scrub solution. The negative corresponds to the normal human serum while the positive corresponds to the people with serum of pooled serum with HCV antibody. The reagent kit provided in the invention has much higher detection sensitivity than the ELISA, which is safe and reliable, easy to operate with low cost, and without any expensive full-automatic chemical luminous measuring apparatus required.

This invention uses immunity compatible chromatography post and enzyme linked immunosorbent assay to detect the remain RactopamineHy-drochloridie in food. It belongs to immunology and food safety detect technology. The method is: prepare muti-clone antibody by immunity antigen synthesis, antigen peridium, animal immunity and antigen depuration. Then put the antigen on to agarose gel to prepare immunity compatible chromatography post. You can enrich the RactopamineHy-drochloridie in the sample by compatible post and gather the eluent of the compatible post, then use linked immunosorbent assay to determine the consistency of the RactopamineHy-drochloridie.

The invention discloses an animal brucella antigen colloidal gold test paper film detection kit, which bases on immunology basic principle of antigen and antibody specific combination, utilizes colloidal gold immunity chromatography technique and silver dye reinforcement technique, uses combined golden yellow staphylococcus A protein (SPA) as gold marking antibody, uses high purity brucella LPS as peridium antigen, and utilizes goat anti-bovine IgG as quality control line to prepare colloidal gold test paper tape. The invention can accurately differentiate species of animal brucella and can synchronously detect animal brucella disease of cattle, sheep and pig species with no crossing reaction and low false negative proportion, negative and positive coincidence ratio of the invention and conventional separation and culture is 100%, detection result can be obtained in 5 min, and the kit need no assistant complicated detection instrument.

This invention relates to kit used to diagnosis hypertriglyceridemia (FHTG). Apolipoprotein A5 (ApoA5) is used as FHTG diagnosis biochemical criterion to make anti ApoA5 single clone antibody amphi. The kit includes enzyme standard anti ApoA5 single clone antibody and unmarked anti ApoA5 single clone antibody, confinig liquid, enzyme substrate solution, peridium buffer solution, termination solution, washing solution and enzyme scale. ApoA5in human body blood is coated by the single antibody, and enzyme anti ApoA5 single clone body is used to recognize ApoA5in human body blood, and they are used to the diagnosis of hypertriglyceridemia to reach efficiency prevention and cure of coronary heart disease and stroke and other cardiovascular disease.

The invention provides an elisa kit for detecting chloramphenicol, comprising an enzyme yoke plate with an encrusting peridium source, an enzyme-labeling substance, a chloramphenicol specific antibody, a chloramphenicol standard solution, a substrate visualization solution, a stopping solution, a thickening and washing solution and a thickening double solution; an encrusting chloramphenicol antigen is obtained by coupling a chloramphenicol hapten and bovine gamma globulin by an active ester method, and an antibody is a polyclonal antibody or a monoclonal antibody. The invention also provides a method for detecting the chloramphenicol in animal-derived food, comprising the following steps: sample pre-treatment; detection by reagents in the kit; and detection result analysis. The elisa kit and the detection method are simple and convenient to operate, have low cost and are suitable for screening a great amount of samples to detect the chloramphenicol in the animal-derived food.

The invention discloses an ELISA method by indirect competition for testing the Gonyautoxin GTX2 and GTX3, which comprises the preparation method of antigen pre-peridium strips and the test method of Gonyautoxin. By utilizing the gained secretory positive cells of the monoclonal antibody with the function of anti-GTX2 and GTX3, the method provided in the invention can prepare in mass production the monoclonal antibody with the function of anti-GTX2 and GTX3, which costs low and can fast, conveniently and sensitively test the content of the GTX2 and GTX3 of the samples. The invention can make fast and sensitive test on the PSP (Paralytic shellfish poison) left in the seafood like fishes and shellfishes on a large scale.

The invention discloses a O, O-dimethyl-O-[-3-methyl-4-amino phenyl] thionic phosphate (FNH2) as fenitrothion hapten, artificial antigen and specific antibody, which is characterized by the following: reducing nitro in the fenitrothion molecular structure into amino under acid condition with zinc powder; reacting to obtain the hapten molecule in connection with carrier protein; diazotizing to couple hapten and protein to obtain immunogen and peridium; using immune animal with immunogen to make the specific antibody.

The invention relates to a hospital checking and diagnostic reagent and the instrument development and application field. The technical scheme includes: (1) preparing serial microglobes of different impedances. 2) making the peridium of the known antigen, antibody and DNA segment on different impedance microglobes, making the surface of different impedance serial microglobes bear light signals, 4) interpreting the mpedance and analysing the quantity of light signal. The advantage is that one determining reagent box can determine multiple kinds of specimen. The infinity of impedance variable of the the impedance variable microglobe liquid biochip etermine the infinity of the detecting items, so as to bring more assay items and the higher sensitivity, besides, different impedance diagnosis microglobes can be preserved for a long time, the technical requirement of the determining device is relatively lower, easier for industrialization and popularization.

The invention belongs to fields of immunochemistry analysis. It discloses an enzyme-uniting immune method and reagent box used in detection of quinoxaline-2-carboxyl acid residues, the method mainly contain medicine reconstruction, the preparation of immunogen, peridium antigen and antibody and pretreatment of sample and building of ELISA detecting method. The reagent box comprises quinoxaline-2-carboxyl acid (QCA) specificity antibody, QCA standard substance, enzyme target-object peridiumed with coupling substance of egg albumin and reaction production of QCA and gamma-aminobutyric acid. The sample is hydrolysed by metaphosphoric acid to discharge QCA, and cleaned by MAX column, derivatized by butyl amine, indirect competition ELISA is adopted in detection. The method and reagent box in the invention has advantages of simpleness, speediness, sensitivity and accuracy, large-lot samples can be detected fast and simultaneously, lowest detecting limit is 0.6 mug/kg.

The invention provides a hapten comprising chlorogenic acid or derivatives thereof, an immunogen of the hapten which can be combined with the carrier material endowed with antigenicity, a conjugate (peridium source) of the hapten which is combined with tag reagent, and an antibody which can resist the immunogen; and the antibody can be combined with the determining position of at least one structural antigen in complete chlorogenic acid. The invention also provides a method and a kit used for testing or measuring the content of the chlorogenic acid in samples, and the usage of the conjugate and the antibody in the tested or measured chlorogenic acid. The invention has specificity to the chlorogenic acid, and can be used for testing the presence of the chlorogenic acid and measuring the content of the chlorogenic acid.

The invention discloses an animal brucella antibody colloidal gold test paper film detection kit, which bases on immunology basic principle of antigen and antibody specific combination, utilizes colloidal gold immunity chromatography technique and silver dye reinforcement technique, uses combined golden yellow staphylococcus A protein (SPA) as gold marking antibody, uses high purity brucella LPS as peridium antigen, and utilizes goat anti-bovine IgG as quality control line to prepare colloidal gold test paper tape. The invention can accurately differentiate species of animal brucella and can synchronously detect animal brucella disease of cattle, sheep and pig species with no crossing reaction and low false negative proportion, negative and positive coincidence ratio of the invention and conventional separation and culture is 100%, detection result can be obtained in 5 min, and the kit need no assistant complicated detection instrument.

The invention discloses an immunodiagnosis kit for detecting III dengue virus NS1 antigen and the application of the kit to the detection of the III dengue virus NS1 antigen. The immunodiagnosis kit for detecting III dengue virus NS1 antigen provided by the invention comprises a microplate coated by peridium monoclonal antibody 1D14A2A10, monoclonal antibody 5D32A17 marked by biotin, positive control, negative control, concentration wash solution, colored solution and stopping solution. The kit can specifically detect that NS1 protein of the III dengue virus and the other three types of serotype dengue virus NS1 have no cross reaction and combines the different antigen sites of the NS1. The immunodiagnosis kit for detecting III dengue virus NS1 antigen can rapidly detect large amounts of samples at the same time, has the characteristics of high specificity, high sensitivity, and the like and important clinical application value, and can rapidly and correctly perform early typing diagnosis to the patients with the dengue virus infection.

The invention belongs to the technical field of improvement, research and development of biological test methods and particularly relates to a test method for hog cholera virus E2 protein quantification. The test method comprises the first step of preparing and purifying a hog cholera virus E2 protein monoclonal antibody, the second step of optimizing peridium and peridium concentration of the hog cholera virus E2 protein monoclonal antibody and the concentration of hog cholera virus E2 protein to be tested and the third step of determining the critical value and the test result. Compared with a conventional method, the qualitative process for quantification through the method requires about 2 hours; on the premise of not influencing the quantification result, the requirements for personnel, instruments and equipment are not high, time and labor are saved, and a novel method is provided for hog cholera virus E2 protein quantification.

The invention discloses a synthetic method of an immunogen for preparing a salmonella cross-type antiboby, and belongs to the technical field of immunoassay. The characteristic that mutant salmonella lipopolysaccharide (Ra-LPS) has no O-specificity side chain is utilized, the EDC and NHS method is adopted for coupling LPS tail end COOH and KLH, and mice sera obtained in an immunization mode have titer on the Ra-LPS and intact LPS. Salmonella thallus peridia of different serogroups are used for detecting the mice sera to obtain the result that all the mice sera have the cross reaction, and it is proved that the immunogen can be used for preparing the salmonella cross-type sera or monoclonal antibody. The synthetic method of the immunogen is used for preparing the salmonella cross-type antiboby, and therefore a solid foundation is laid for detecting the salmonella in the bacterial genus level.

The invention discloses an ELISA (Enzyme Linked Immuno-Sorbent Assay) knit based on Nsp10 protein in PRRSV (porcine reproductive and respiratory syndrome virus), which comprises 50-200 ml of recombined protein peridium, 50-200 ml of positive reference, 50-200 ml of negative reference, 50-200 ml of sealing liquid, 50-200 ml of enzyme labeled secondary antibody, 50-200 ml of substrate liquid and 50-200 ml of stop solution. The purified Nsp10 recombined protein is taken as envelope antigen, and an ELISA method is established to optimize the reaction condition and research an ELISA knit based on Nsp10 protein in PRRSV; via comparing the ELISA detection result with the positive reference and the negative reference, a detection conclusion is obtained; a detection conclusion is obtained through the comparison between the ELISA detection result and the negative reference and the positive reference; the conclusion can basically distinguish PRRS inactivated vaccine immune swinery from inapparent infected swinery; therefore, reference data is provided for the clinical diagnosis and the prevention and control of the PRSS; according to the detection result, the uninfected swinery is selectively immune; therefore, the immune aimlessness can be reduced, and the economic loss is effectively avoided. The ELISA knit has the characteristics of good specificity, sensitivity, repeatability, and the like, and is good in use effect.

The invention relates to an enzyme-linked immunity reagent kit which detects an aquatic animal spiroplasma; the reagent kit comprises peridium solution, cleaning solution, calf serum, one-antiserum, enzyme binder working solution, visualization solution, stop solution, positive control, negative control and an enzyme-labeled plate; the visualization solutions are tetramethy benzidine, citric acid-phosphate buffer solution and hydrogen peroxide; the positive control is a sample comprising spiroplasma; the negative control is the sample which doesn't comprise spiroplasma; the one-antiserum is polyclonal antibody of the aquatic animal spiroplasma; the enzyme binder working solution is a horseradish peroxide enzyme-labeled goat anti-rabbit IgG polyclonal antibody; indirect ELISA method is mainly adopted to detect the spiroplasmas in the blood sample and muscle tissue sample of an aquatic animal. The reagent kit has the advantages of convenience, swiftness, economy, high sensitivity, strong specificity and sound repeatability; furthermore, the reagent kit is in favor of popularization and application and can effectively detect the spiroplasmas in blood sample and muscle tissue sample.

The invention discloses a ray irradiation method for improving peridium quality of polystyrene micropore plate, which is realized by the following schemes: aligning a bottle opening of the polystyrene micropore plate to a cobalt source plate, implementing gamma-ray irradiation to the polystyrene micropore plate by the cobalt source plate, controlling the minimum absorption dose of the polystyrene micropore plate to be 4.0KGY and controlling the unevenness of irradiation to be 1.3-1.4, wherein the gamma-ray has an energy of 1.33MeV,. The invention uses the characteristics of higher energy and high penetration of 60Cor ray, and performs irradiation processing to the polystyrene micropore plate using certain irradiation dose and special technique, wherein the irradiated micropore plate is suitable for all types of antigen molecules, can effectively improve absorption of the micropore plate to micromolecule polypeptide and obviously improve the sensitivity and uniformity for detecting enzyme linked immunosorbent (ELISA). The method has the advantages of simple operation, stable quality, lower cost, and the like, thereby having enormous promotion and application value.