The invention discloses diethylstibestrol
hapten and complete
antigen and a preparation method thereof. The preparation method comprises the following steps: under conditions of normal temperature andpresence of a catalyst, performing reaction on
diethylstilbestrol and monochloroacetate in an
organic solvent, terminating the reaction with icy water, treating unreacted
diethylstilbestrol with an organic extraction agent, and acidizing a water phase with an acid, so as to obtain white precipitate, namely diethylstibestrol
hapten. By adopting the preparation method, the catalyst, the reaction time and the like in synthesis are optimized, a simple and convenient method without heating or pH value control is provided, and
hapten can be prepared within a short time; by the virtue of different
dissolution properties of a product and a substrate, unreacted
diethylstilbestrol substances are removed by using an extraction method, the obtained diethylstilbestrol substances are recycled and continuously used, and the reaction substrate is saved; finally the diethylstilbestrol hapten of high purity is prepared by using a
hydrochloric acid acidification
crystallization method; complete antigenis prepared from the diethylstibestrol hapten and a
carrier protein through connection.