47results about How to "Retain chemical structure" patented technology

The invention discloses a hapten, and a preparation method and application thereof, particularly a methyl parathion hapten. The invention also discloses a preparation method and application of the hapten. The kit quick detection product established on the basis of the methyl parathion hapten is convenient to use and low in detection cost; and the detection method is efficient, accurate and quick, can be used for simultaneously detecting mass samples, and is suitable for on-site supervision of methyl parathion residues in corn and apple samples and screening of mass samples.

The invention relates to a method for preparing vital wheat gluten flour, and belongs to processing methods of edible protein. The method comprises the following steps: a. adding water to wheat flour to be made into flour dough; b. naturally curing and washing the flour dough with the water to obtain a gluten flour clot; and c. freezing and drying the gluten flour clot to obtain the vital wheat gluten flour, wherein, the freezing temperature is minus 5-minus 6 DEG C, and the freezing time is 8-10 hours.

The invention discloses a multi-scale continuous calculation method for solving the mesomechanics performance of an energetic material. The method comprises the following steps: respectively carryingout molecular dynamics simulation and coarse graining molecular dynamics simulation on an explosive crystal component and a polymer binder component in PBX; converting a real PBX structure into a random round particle digital model through an effective approximation method; connecting key parameters from microcosmic scale to mesoscopic scale; calculating the mechanical properties of particles PBXunder a mesoscopic scale, establishing a representative volume meta-model of PBX on the mesoscopic scale by adopting a material point method, researching the mechanical behaviors of PBX under uniaxialcompression to obtain the elasticity modulus and state equation parameters of PBX, and inputting the elasticity modulus and state equation parameters into a continuum of a macroscopic scale as parameters for calculation. According to the invention, a composite energetic material cross-scale continuous calculation method completely based on theoretical calculation is realized from microcosmic scale to microcosmic scale and from single component to compound, and the method has important guiding significance for safety design of explosives.

The invention provides a preparation method of a sildenafil artificial antigen and antibody, and relates to a preparation method of an artificial hapten, antigen and antibody maximizing the retention of a sildenafil structure. A sildenafil hapten prepared by a chemical synthesis method and a carrier KLH protein are connected and synthesized into an artificial antigen; and an antibody is prepared by the following steps: immunizing animals with the artificial antigen, taking blood, separating out antiserum and purifying. By designing and synthesizing the hapten and artificial antigen having a sildenafil structure, the invention highlights the molecule-specific antigenic determinant, overcomes difficulties in chemical synthesis, and produces the high-affinity antibody through animal immunization inducement. Compared with other similar methods, the invention has the characteristics of specificity, sensitivity, accuracy, high speed, convenience, low cost and the like; and the designed and synthesized hapten lays a foundation for the preparation of the antibody having favorable specificity.

The invention discloses a O, O-dimethyl-O-[-3-methyl-4-amino phenyl] thionic phosphate (FNH2) as fenitrothion hapten, artificial antigen and specific antibody, which is characterized by the following: reducing nitro in the fenitrothion molecular structure into amino under acid condition with zinc powder; reacting to obtain the hapten molecule in connection with carrier protein; diazotizing to couple hapten and protein to obtain immunogen and peridium; using immune animal with immunogen to make the specific antibody.

The invention discloses a semi-antigen, a preparation method and applications thereof, and specifically relates to a dexamethasone semi-antigen. The invention also discloses a preparation method of the semi-antigen and applications thereof. The rapid detection kit product based on the dexamethasone semi-antigen has the advantages of user-friendliness, low detection cost, high efficiency, accuracy and rapidness, is capable of detecting a large amount of samples at the same time, and is suitable for on-site supervision of dexamethasone residual in the animal source food and screening of a large amount of samples.

The invention discloses acetamiprid hapten and a preparation method thereof as well as application. The acetamiprid hapten is characterized in that the acetamiprid hapten is obtained by reacting N-noracetamiprid and aminobutyric acid; and the acetamiprid hapten is obtained by coupling acetamiprid hapten and carrier protein. The hapten prepared by the preparation method shows a specific acetamipridantigen determinant, so that a high-specificity acetamiprid monoclonal antibody is possibly screened out. The generated antibody is high in specificity and sensitivity, can be used for establishing an enzyme linked immunoadsorption assay method and a colloidal gold test paper quick measuring method, so that quick detection on acetamiprid in tobaccos and food is realized.

The invention provides preparation of a semi-antigen and an artificial antigen and an antibody against herbicide anilofos, which relates to the semi-antigen, the artificial antigen and the antibody which have a basic structure of the herbicide anilofos. According to the invention, the antibody with good specificity to the herbicide anilofos is prepared by using an easy and convenient method. The antibody is prepared through the following steps: synthesizing the semi-antigen from N-chloracetyl-N-isopropyl-4-chloroaniline and mercaptoacetic acid; respectively connecting the semi-antigen with bovine serum albumin (BSA) and horseradish peroxidase (HRP) to synthesize the artificial antigen and an enzyme-labeled antigen; and subjecting the artificial antigen to animal immunization, blood drawing, separation of antiserum and purification so as to prepare the antibody. The antibody is stable and has good specificity and sensitivity; the synthetic method for the antibody is simple; and the antibody can be used for rapid immunodetection of residual herbicide anilofos in the environment, agricultural products and foodstuffs and has a good application prospect.

The invention discloses an antigen, an antibody and an enzyme-linked immunosorbent assay (ELISA) kit of amantadine. Firstly, an amantadine hapten is prepared, wherein the molecular structural formulaof the amantadine hapten is as shown in formula (I) (The formula is shown in the description.); then, the amantadine hapten is coupled with carrier protein to obtain an artificial antigen; and then, amonoclonal antibody of amantadine is obtained through a hybridoma technique, thereby establishing a competitive ELISA method and kit according to the monoclonal antibody of amantadine. The ELISA kitof amantadine, disclosed by the invention, can be used for detecting the content of amantadine in an animal tissue sample, the detection limit is 0.10mu g / kg, IC50 is 0.79mu g / L, and the linear detection range is 0-16.2mu g / L; and the method is simple, convenient and quick, is low in cost, high in sensitivity and highstrong in specificity, and is suitable for on-site quick detection.

The invention discloses a yellow fluorescence carbon dot, a preparation method of the yellow fluorescence carbon dot, and an application of the yellow fluorescence carbon dot to label-free detection of quinolone antibiotics. The carbon dot is a yellow fluorescence carbon dot taking o-phenylenediamine and aminobutyric acid as the raw materials and prepared through a one-step hydro-thermal synthesismethod. The preparation method of the carbon dot is simple and convenient, and the prepared carbon dot is stable in optical property and good in water solubility and dispersity. The carbon dot, as afluorescence probe, is used to rapidly sensitively detect the quinolone antibiotics without label due to the special surface structure. When the quinolone antibiotics exist, the fluorescence of the carbon dot is effectively quenched by the quinolone antibiotics, and the content of the quinolone antibiotics in a sample is detected according to the variation degree of the fluorescence. Compared withthe traditional method, the provided quinolone antibiotics detection method is simple, convenient and fast, no label is extraordinarily modified, the sample is directly detected, and the method has more advantages in the practical application.

The invention provides a preparation method of a nandrolone artificial antigen and antibody, and relates to a preparation method of an artificial hapten, antigen and antibody maximizing the retention of a nandrolone structure. A nandrolone hapten prepared by a chemical synthesis method and a carrier BSA protein are connected and synthesized into an artificial antigen; and an antibody is prepared by the following steps: immunizing animals with the artificial antigen, taking blood, separating out antiserum and purifying. By designing and synthesizing the hapten and artificial antigen having a nandrolone structure, the invention highlights the molecule-specific antigenic determinant, overcomes difficulties in chemical synthesis, and produces the high-affinity antibody through animal immunization inducement. Compared with other similar methods, the invention has the characteristics of specificity, sensitivity, accuracy, high speed, convenience, low cost and the like; and the designed and synthesized hapten lays a foundation for the preparation of the antibody having favorable specificity.

The invention discloses a semi-antigen and a preparation method and application thereof and specifically relates to an amikacin semi-antigen. The invention further discloses a preparation method and application of the amikacin semi-antigen. A rapid detection kit product established on the basis of the amikacin semi-antigen has the advantages of convenient usage, low detection cost, usage of highly efficient, accurate and rapid detection methods and capacity of simultaneous on-site monitoring of amikacin residue and screening of considerable samples.

The invention relates to a preparation method and the application of a hapten and an antigen of butralin. The preparation method is characterized in that the hapten of butralin is obtained through thereaction of 4-chloro-3,5-dinitrophenylacetic acid and butylamine, and the antigen of butralin is obtained by coupling the hapten of butralin with a carrier protein. The antigen prepared by the methodprovided by the invention presents a specific butralin antigenic determinant, which ensures the probability of screening out a high-specificity butralin monoclonal antibody. A generated antibody hashigh specificity and sensitivity, and can be used for establishing an enzyme-linked immunosorbent assay method and a colloidal gold test paper rapid assay method, therefore, rapid detection of butralin in tobacco and food is achieved.

The invention discloses a hapten, and a preparation method and application thereof, particularly a sulfonamide hapten. The invention also discloses a preparation method and application of the hapten. The kit quick detection product established on the basis of the sulfonamide hapten is convenient to use and low in detection cost; and the detection method is efficient, accurate and quick, can be used for simultaneously detecting mass samples, and is suitable for on-site supervision of sulfonamide residues in animal-derived samples and screening of mass samples.

The invention relates to a preparation method and application of a safrole hapten and a safrole antigen. The preparation method is characterized in that the safrole hapten is obtained by carrying outreaction on 5-(2-bromo-2-propene-1-yl)-1,3-benzodipentene and aminopropionic acid; and the safrole antigen is obtained by coupling the safrole hapten and carrier protein. The antigen prepared throughthe preparation method shows a specific safrole antigenic determinant, and a safrole monoclonal antibody can be prepared through immune animals; and the generated antibody is high in specificity and high in sensitivity and can be used for preparing an enzyme-linked immunosorbent assay kit for detecting safrole, a colloidal gold test strip and a time-resolved fluorescence immunochromatographic teststrip, so that the safrole in tobacco flavors and essences, tobacco, tobacco products and food can be rapidly detected. The formula of the safrole hapten is shown in the description.

The invention relates to preparation of a semiantigen, an artificial antigen and an antibody with a basic structure of 5-hydroxymethyl furfural. According to the invention, the antibody with good specificity to 5-hydroxymethyl furfural is prepared by using an easy and convenient method. The antibody is prepared through the following steps: synthesizing the semiantigen from 5-hydroxymethyl furfural and succinic anhydride; respectively connecting the semiantigen with KLH and HRP to synthesize the artificial antigen and an enzyme-labeled antigen; and subjecting the artificial antigen to animal immunization, blood drawing, separation of antiserum and purification so as to prepare the antibody. The antibody is stable and has good specificity and sensitivity; the synthetic method for the antibody is simple; and the antibody can be used for rapid immunodetection of 5-hydroxymethyl furfural in foodstuffs and has a good application prospect.

The invention discloses a hapten, as well as a preparation method and application thereof, and specifically relates to a furazolidone metabolite hapten. The invention also discloses a preparation method of the hapten and application thereof. A kit quick detection product established based on the furazolidone metabolite hapten is convenient to use, low in detection cost, and efficient, accurate and quick in detection method; the kit quick detection product is capable of detecting a large batch of samples at the same time, and thus suitable for on-site supervision of furazolidone metabolite residues in animal derived food and screening of lots of samples.

The invention provides a gemfibrozil hapten and an artificial antigen. Molecular structural formulas of the gemfibrozil hapten and the artificial antigen are correspondingly as shown in the description. The invention further discloses preparation methods for the gemfibrozil hapten, the artificial antigen and an antibody prepared from the gemfibrozil hapten and the artificial antigen and purposes of the gemfibrozil hapten and the antibody. The antibody which is finally prepared by the preparation method provided by the invention has the advantages of high detection sensitivity and high specificity.

The invention discloses hapten and a preparation method and application thereof and particularly relates to spinosad hapten and a preparation method and application thereof. The spinosad hapten has the advantages that the chemical structure of spinosad is retained to a maximum degree, -COOH capable of being coupled with protein is introduced through chemical synthesis modification, and the synthesizing method is simple and high in product purity and yield; an antigen system suitable for animal immunization is prepared by using the spinosad hapten as the raw material, an antibody obtained after the animal immunization is good in titer, specificity and affinity, and the antibody can be used for preparing an enzyme linked immunosorbent assay kit; the kit is simple to operate, low in detection cost, efficient, accurate and fast in detection method, capable of simultaneously detecting a large batch of samples and applicable to the field monitoring of spinosad residues in animal-derived food and the screening of a large amount of samples; the spinosad hapten plays an important role in spinosad detection.

The invention discloses preparation and application of a pendimethalin hapten and antigen. Preparation of the pendimethalin hapten and antigen is characterized in that the pendimethalin hapten is prepared from 3-(1-ethyl aminopropylamino)-6-methyl-2,4-dinitrobenzaldehyde and 3-hydrazino propionic acid which are reacted; the pendimethalin antigen is obtained by coupling the pendimethalin hapten andcarrier protein. The prepared antigen presents a specific pendimethalin antigenic determinantantigen epitope, so that screening out a high-specificity pendimethalin monoclonal antibody becomes possible; the generated antibody is high in specificity and high in sensitivity, and can be used for building an enzyme-linked immunosorbent assay method and a colloidal gold test strip rapid determinationmethod, so that pendimethalin in tobacco and food is fast detected.

The invention discloses diethylstibestrol hapten and complete antigen and a preparation method thereof. The preparation method comprises the following steps: under conditions of normal temperature andpresence of a catalyst, performing reaction on diethylstilbestrol and monochloroacetate in an organic solvent, terminating the reaction with icy water, treating unreacted diethylstilbestrol with an organic extraction agent, and acidizing a water phase with an acid, so as to obtain white precipitate, namely diethylstibestrol hapten. By adopting the preparation method, the catalyst, the reaction time and the like in synthesis are optimized, a simple and convenient method without heating or pH value control is provided, and hapten can be prepared within a short time; by the virtue of different dissolution properties of a product and a substrate, unreacted diethylstilbestrol substances are removed by using an extraction method, the obtained diethylstilbestrol substances are recycled and continuously used, and the reaction substrate is saved; finally the diethylstilbestrol hapten of high purity is prepared by using a hydrochloric acid acidification crystallization method; complete antigenis prepared from the diethylstibestrol hapten and a carrier protein through connection.

The invention discloses a preparation method of a bisphenol A hapten and a bisphenol A complete antigen. The preparation method comprises the steps of enabling bisphenol A, which is taken as a raw material, to react with monochloroacetate in presence of a catalyst-potassium hydroxide / sodium hydroxide, removing the unreacted bisphenol A by using ethyl acetate, then acidizing by using hydrochloric acid to obtain a product, filtering, washing, and drying to obtain the bisphenol A carboxylated hapten; coupling the bisphenol A hapten with a carrier protein OVA by using a carbodiimide method so as to prepare the bisphenol A complete antigen. The preparation method provided by the invention is simple, convenient and rapid, and greatly shortens the reaction time on the basis of guaranteeing the product purity and yield; furthermore, after the preparation method is adopted, heating and pH control are not needed, so that the industrial production cost is lowered.

The invention provides an ALT (atenolol) hapten and an artificial antigen. The molecular structural formula of the hapten is shown in the description. The invention further discloses preparation methods of the hapten, the artificial antigen and the antibody prepared from the antigen as well as applications of the hapten and the antibody. The final prepared antibody has high detection sensitivity and strong specificity.

The invention discloses an estrogen hapten, a complete antigen and a preparation method thereof. The method comprises: reacting estrols and monochloroacetate in an organic solvent at normal temperature and in the presence of a catalyst, terminating the reaction with ice water, taking the unreacted estrols of the organic extractant, and using acid Acidification produces a white precipitate, which is the estrol hapten. By optimizing the catalyst and reaction time in the synthesis, this method provides a simple method without heating and controlling the pH, and the hapten can be obtained in a short time; the unreacted product and the substrate are used to remove the unreacted hapten by using the extraction method. At the same time, the obtained estrols can continue to be used through recovery, saving the reaction substrate; finally, the method of crystallization by hydrochloric acid acidification can obtain high-purity estrols haptens; Haptens are linked to carrier proteins to obtain complete antigens.

The invention discloses a semi-antigen, a preparation method and applications thereof, and specifically relates to a dexamethasone semi-antigen. The invention also discloses a preparation method of the semi-antigen and applications thereof. The rapid detection kit product based on the dexamethasone semi-antigen has the advantages of user-friendliness, low detection cost, high efficiency, accuracy and rapidness, is capable of detecting a large amount of samples at the same time, and is suitable for on-site supervision of dexamethasone residual in the animal source food and screening of a large amount of samples.

The invention discloses a hapten, and a preparation method and application thereof, particularly a methyl parathion hapten. The invention also discloses a preparation method and application of the hapten. The kit quick detection product established on the basis of the methyl parathion hapten is convenient to use and low in detection cost; and the detection method is efficient, accurate and quick, can be used for simultaneously detecting mass samples, and is suitable for on-site supervision of methyl parathion residues in corn and apple samples and screening of mass samples.

A preparation method and application of coumarin hapten and antigen, characterized in that: the coumarin hapten is obtained by acetal reaction of coumarin; the coumarin antigen is obtained by coumarin hapten and Carrier protein coupling is obtained. The antigen prepared by the invention presents specific coumarin antigenic determinants, and the coumarin monoclonal antibody can be prepared by immunizing animals. The antibody produced has high specificity and high sensitivity, and can be used to prepare enzyme-linked immunosorbent assay kits, colloidal gold test strips and time-resolved fluorescent immunochromatographic test strips for the detection of coumarin, thereby realizing the detection of flavors in tobacco, flavors and fragrances and food. Rapid detection of soylin.

The invention discloses an antigen, an antibody and an enzyme-linked immunosorbent assay (ELISA) kit of amantadine. Firstly, an amantadine hapten is prepared, wherein the molecular structural formulaof the amantadine hapten is as shown in formula (I) (The formula is shown in the description.); then, the amantadine hapten is coupled with carrier protein to obtain an artificial antigen; and then, amonoclonal antibody of amantadine is obtained through a hybridoma technique, thereby establishing a competitive ELISA method and kit according to the monoclonal antibody of amantadine. The ELISA kitof amantadine, disclosed by the invention, can be used for detecting the content of amantadine in an animal tissue sample, the detection limit is 0.10mu g / kg, IC50 is 0.79mu g / L, and the linear detection range is 0-16.2mu g / L; and the method is simple, convenient and quick, is low in cost, high in sensitivity and highstrong in specificity, and is suitable for on-site quick detection.

A preparation method and application of a pendimethalin hapten and an antigen, characterized in that: the pendimethalin hapten is composed of N-(1-ethylpropyl)-3,4-dimethylaniline and Ethyl 4‑bromobutyrate is reacted to generate alkylated N‑(1‑ethylpropyl)‑3,4‑dimethylaniline, which is then reacted with concentrated nitric acid to generate nitroalkylated N‑(1‑ethylpropyl) )-3,4-dimethylaniline, and then obtained by reacting with potassium hydroxide; the pendimethalin antigen is obtained by coupling pendimethalin hapten with carrier protein. The antigen prepared by the invention presents specific pendimethalin antigenic determinant, which makes it possible to screen highly specific pendimethalin monoclonal antibodies. The antibody produced has high specificity and high sensitivity, and can be used to establish an enzyme-linked immunosorbent assay method and a rapid determination method of colloidal gold test paper, thereby realizing the rapid detection of pendimethalin in tobacco and food.

The invention discloses a streptomycin hapten, as well as a preparation method and application thereof, and in particular relates to a streptomycin hapten. The invention further discloses a preparation method and an application of the streptomycin hapten. A quick detection enzyme-linked immune kit product built based on the streptomycin hapten is convenient to use, low in detection cost and efficient, accurate and quick in detection, can be used for simultaneously detecting large batches of samples, and is suitable for site monitoring and streptomycin residue in animal derived foods and screening of lots of samples.