Diethylstibestrol hapten and complete antigen and preparation method thereof

Abstract

The invention discloses diethylstibestrol hapten and complete antigen and a preparation method thereof. The preparation method comprises the following steps: under conditions of normal temperature andpresence of a catalyst, performing reaction on diethylstilbestrol and monochloroacetate in an organic solvent, terminating the reaction with icy water, treating unreacted diethylstilbestrol with an organic extraction agent, and acidizing a water phase with an acid, so as to obtain white precipitate, namely diethylstibestrol hapten. By adopting the preparation method, the catalyst, the reaction time and the like in synthesis are optimized, a simple and convenient method without heating or pH value control is provided, and hapten can be prepared within a short time; by the virtue of different dissolution properties of a product and a substrate, unreacted diethylstilbestrol substances are removed by using an extraction method, the obtained diethylstilbestrol substances are recycled and continuously used, and the reaction substrate is saved; finally the diethylstilbestrol hapten of high purity is prepared by using a hydrochloric acid acidification crystallization method; complete antigenis prepared from the diethylstibestrol hapten and a carrier protein through connection.

Description

Technical field

[0001] The present invention relates to the field of immunochemistry and detection technology, in particular to an estradiol hapten, a complete antigen and a preparation method thereof, in particular to diethylstilbestrol (DES), hexestrol (HEX) and diethylstilbestrol (DIS) Preparation method of hapten. Background technique

[0002] Environmental estrogen refers to a class of compounds that have estrogenic effects, can imitate natural estrogen, and interfere with endocrine function. Diethylstilbestrol (DES), Hexestrol (HEX) and Diethylstilbestrol (DIS) are non-steroidal anabolic hormones, which can promote the increase of nitrogen retention in animals, increase protein deposition and reduce fat deposition, because of their simple structure , Easy to synthesize, low cost, etc., and is used to promote the growth of livestock and poultry, increase lean meat rate and so on. Because DES, HEX, DIS, etc. are all environmental estrogens, they can interfere with or block ...

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