116 results about "Diethylstilbestrol" patented technology

The invention relates to a making method and an application of a CdS sensitized TiO2 environmental estrogen photoelectrochemical sensor. The method uses TiO2 as an antigen capture substrate material, a CdS photoelectric active material is in situ generated on the surface of a Cd2<+> functional TiP nanomaterial maker modified electrode through a direct Na2S dropping technology, and CdS is irritated by an LED lamp of visible light wavelength to be converted to a photoelectric current signal. The substrate material TiO2 can be well matched with CdS energy band, so the photocurrent conversion signal of CdS is further improved, thereby the competitive photoelectrochemical immunosensor for ultra sensitive detection of estradiol, estriol, diethylstilbestrol, bisphenol A, nonylphenol, oestrone and other environmental pollutants is made.

The invention relates to an electrochemical check method of glass-carbon electrode decoration of estromon, belonging to electrochemical analysis technical field, comprising that electrode pretreatment, nanometer gold sol preparation, polyethylene imine-nanometer gold decorated glass carbon electrode preparation, more particularly comprising that preparing polyethylene imine decorated glass-carbon electrode and preparaing polyethylene imine nanometer gold decorated electrode, and using the polyethylene imine nanometer gold decorated electrode to test the content of estromon. The inventive decoration of the working electrode is simple and repeatable, while the decorated electrode has strong selective collection on estromon, better test repeatability and high sensitivity, while the check limit can reach 0. 001mug/g.

The invention provides a kit for detecting diethylstilbertrol and detection method thereof, belonging the the time resolution fluoroimmunoassay (TRFIA) technical field, capable of detecting the content of diethylstilbestrol (DES) in animal-source food, blood, urine and feedstuff. The kit uses the TRFIA to detect the DES and the mark immune response is the base of the detection. A micropore plate is coated by DES-carrier protein and added with DES standard or sample and then added with DES antibody. The free DES and the DES-carrier protein on the micropore plate complete the DES antibody and the DES antibody which is not bonded with the free DES or the DES-carrier protein is cleared away and added with the EU3+-goat anti-rabbit antibody, after labeling the immune reaction, the non-bonded EU3+-goat anti-rabbit antibody is cleared away. After adding the reinforcement liquid, the fluorescence intensity cps is detected using a time resolution fluorescence instrument and the fluorescence intensity is inversely proportional to the DES concentration in sample and the content of DES in sample can be determined according to the standard curve. The DES detection kit has features of simple structure, convenient use, cheapness, high sensitivity up to 1ng/mL.

The invention discloses a diethylstilbestrol chemiluminescent detection kit, comprising a detection plate and a reagent. The reagent comprises an antibody which is a monoclonal antibody prepared by using a conjugate of diethylstilbestrol and bovine serum albumin as an immunogen. The diethylstilbestrol detection kit can realize quick and large-scale detection and has very high specificity and sensitivity, wherein the detection sensitivity reaches 0.016 ppb.

The invention relates to a making method and an application of a CdS sensitized TiO2 environmental estrogen photoelectrochemical sensor. The method uses TiO2 as an antigen capture substrate material, a CdS photoelectric active material is in situ generated on the surface of a Cd2<+> functional TiP nanomaterial maker modified electrode through a direct Na2S dropping technology, and CdS is irritated by an LED lamp of visible light wavelength to be converted to a photoelectric current signal. The substrate material TiO2 can be well matched with CdS energy band, so the photocurrent conversion signal of CdS is further improved, thereby the competitive photoelectrochemical immunosensor for ultra sensitive detection of estradiol, estriol, diethylstilbestrol, bisphenol A, nonylphenol, oestrone and other environmental pollutants is made.

Oral dosage forms as a biodegradable, water soluble film for delivering pharmaceutically active agents, particularly diethylstilbestrol and pharmaceutically acceptable salts thereof to patients through insertion into the mouth of patient and methods for administering pharmaceutically active agents to patients by insertion into the mouth to provide selective uptake of said agents through the mucosa and thus avoiding the gastrointestinal tract.

The invention discloses a method for detecting diethylstilbestrol and a special chemiluminescent immunoassay kit thereof. The chemiluminescent immunoassay kit for detecting diethylstilbestrol comprises a diethylstilbestrol specific antibody, a coating antigen and a standard solution, wherein the coating antigen is a conjugate of diethylstilbestrol hapten and carrier protein, and the constitutional formula of the coating antigen is as shown in a formula I. The detection method has the characteristics of simple preprocessing of samples, easy operation, low cost, high specificity, high sensitivity, high accuracy, and the like, and is capable for on-site monitoring and suitable for screening a large amount of samples.

The invention discloses a protein chip for detecting multiple veterinary drug residues and a kit thereof. The protein chip comprises a substrate and dot coatings of veterinary drug antibody, wherein the dot coatings are distributed in an array type, the substrate is a glass substrate or a membrane substrate; and the dot coatings of the veterinary drug antibody refer to eight types of veterinary drug antibodies, i.e. an anti-chloramphenicol antibody, an anti-chlorpromazine antibody, an anti-colchicin antibody, an anti-dapsone antibody, an anti-stilboestrol antibody, an anti-dimetridazole antibody, an anti-furazolidone antibody and an anti-metronidazole antibody which are uniformly distributed and in dot lattice on the substrate. The protein chip can obtain the reaction results with multiple indexes by only one-step reaction and can be suitable for simultaneously detecting eight veterinary drugs, i.e. chloramphenicol, chlorpromazine, colchicin, dapsone, stilboestrol, dimetridazole, furazolidone and metronidazole which are contained in a sample, thereby ensuring the safety and the sanitation of a large quantity of meat products.

The invention discloses a method for measuring residual quantity of diethylstilbestrol in aquatic products by utilizing a liquid chromatogram fluorescent method, comprising the following steps: (1) weighing 100g of ground aquatic product samples in a 50mL plastic centrifuge tube, adding 20mL of acetic ether, homogenizing for 230s, centrifuging for 10min with the speed of 7000r/min, moving the acetic ether layer to a 100mL pear-shaped flask, utilizing 2mL of normal hexane and acetic ether with a volume ratio of 85:15 to dissolve residue, and then stirring for 6min; (2) activating an SLH column by utilizing 5mL of normal hexane and 5mL of acetic ether, adding extracting solution on the SLH column, utilizing 10mL of normal hexane and acetic ether with the volume ratio of 82:20 to elute by controlling the flow rate at 2mL/min, collecting eluent, carrying water bath at the temperature of 450 DEG C, and then carrying out nitrogen drying till the original state; and (3) adding 1mL of sulfuric acid to the centrifuge tube of nitrogen drying, then adding 8mL of water in the centrifuge tube, utilizing 1mL of mobile phase for metering volume so as to be measured by an efficient liquid phase chromatogram. The method for measuring residual quantity of diethylstilbestrol in aquatic products has advantages of low cost, simple method, and low measuring limit, is suitable for popularization and use in the production and practice and is a technology demanded by the market, and has positive social meaning and social demand.

The invention discloses an enzyme immunity adsorption check agent box used for analyzing the left depo-estromon, characterized in composed of an enzyme mark plate with the depo-estromon antigen, a sponge support, a concentrate washing agent (PBST), a depo-estromon standard product, a depo-estromon single-clone antibody, an enzyme mark two-antibody, a develop liquid and a reaction end liquid. In the check, the covered antigen absorbed on the hole wall of the enzyme mark plate competes with the object depo-estromon to react with the antibody, while the result is displayed via the development, checks the depo-estromon in known density and draws a standard curvature, to obtain the density of the object depo-estromon. The invention can accurately check the left depo-estromon in water and meat, with simple pretreatment, low time consumption, batch application, and low cost, while the agent has long service life and non radioactive pollution.

The invention discloses a preparation method of a filter medium for removing diethylstilbestrol in water. The preparation method comprises the following steps: a) mixing raw materials containing ultrahigh molecular weight polyethylene, active carbon, sepiolite powder, attapulgite powder and hole forming agent, wherein the weight ratio of the ultrahigh molecular weight polyethylene, the active carbon, the sepiolite powder, the attapulgite powder and the hole forming agent is 200-350:50-100:40-100:50-100:50-120; and b) pressing, sintering and cooling the obtained mixture in a mould. The invention also discloses the filter medium prepared according to the preparation method. Compared with the prior art, the invention can remove the diethylstilbestrol in the water with a removal rate of over 95 percent so as to achieve the aim of improving the water quality; in addition, the method is simple, and the raw materials for preparing the filter medium are widely available and have low cost. Thetechnical scheme is in particular applicable for the treatment of drinking water polluted by the diethylstilbestrol.

The invention discloses an efficient induction method and a microsatellite molecular marker identification method of Nile tilapia pseudo-female fishes. Different doses of estrogen diethylstilbestrol (DES) are used for sex reversal processing of different groups of Nile tilapia fishes to establish the efficient induction method of the Nile tilapia pseudo-female fishes; and a newly-developed microsatellite molecular marker tlp-23-sd and a primer thereof are further used for identification of the Nile tilapia pseudo-female fishes to establish the microsatellite molecular marker rapid identification method of the pseudo-female fishes, wherein the microsatellite molecular marker tlp-23-sd is closely linked with the sex. The efficient induction method is high in pseudo-female fish induction efficiency, and the microsatellite molecular marker rapid identification method is rapid, accurate and reliable in identification, and can be used for the production and identification of the Nile tilapia pseudo-female fishes.

The invention discloses a GC-MS (gas chromatography-mass spectrum) measuring method of phenolic compounds in surface water, and belongs to the technical field of organic pollutants in water environment. The method is characterized in that in the water sample filtering, solid-phase extraction, purification and concentration processes, an ion monitoring GC-MS method is used for measurement; an internal standard method is used for quantification. The measuring method has the advantages that the detection on four kinds of phenolic compounds including octyl phenol, nonyl phenol, bisphenol A and diethylstilbestrol in the surface water has higher sensitivity, accuracy and precision; the measuring method hopefully becomes a new detection standard.

A method for detecting diethylstilbestrol belongs to the field of analytical chemistry, wherein the method includes the followings: pre-treating the sample to be detected, preparing the sample solution and then carrying out the following treatments: adding sample solution at one end point of the paper chromatography strip; placing the paper chromatography strip in the normal hexane-ethanol developing solvent; taking out the paper chromatography strip when the front end of the developing solvent is adjacent to the front end of the paper chromatography strip; air-drying, wherein the normal hexane-ethanol developing solvent by volume includes: 1 to 20 parts of normal hexane, 0.1 to 5 parts of ethanol; spraying the potassium ferricyanide-ferric trichloride-H2O2 on the chromatography strip; color displaying, wherein the potassium ferricyanide-ferric trichloride-H2O2 is as follows: mixing the 0.01 to 5 mol/L aqueous solution of the potassium ferricyanide and the 0.01 to 5 mol/L aqueous solution of ferric trichloride evenly; then adding 0.001% to 5% of the H2O2 by volume 1 to 1; and the volume of the H2O2 is 0 to 10% of the total volume of the solution. The invention has the advantages of simple method and device, fast detecting speed and low detecting cost, and can be used for fastly detecting the DES in food on the spot.

A chemical reagent box applies enzyme linked immunosorbent assay method to detect diethylstilbestrol. The cony IgG sensitizer is enveloped on the enzyme scale plate and the two are reacted through hatching, washnig, sample diluent and enzyme scale antigen adding after diethyl stilbestrol sensitizer is added. The detection is finished by washing, adding enzyme-substrate material to display coloure and using enzyme scale meter to detect A 450 nm.

The invention discloses a synthesis method of diethylstilbestrol compound methyl pigeon pea ketonic acid A. The synthesis method comprises the steps: esterifying a compound with a formula (I) to generate a compound with a formula (II); reducing the compound with the formula (II) to generate a compound with a formula (III); carrying out substitution on the compound with the formula (III) to generate a compound with a formula (IV); carrying out substitution on the compound with the formula (IV) to generate a compound with a formula (V); carrying out alkylation on the compound with the formula (V) to generate a compound with a formula (VI); carrying out esterolysis on the compound with the formula (VI) to generate a compound with a formula (VII); carrying out decarboxylation on the compound with the formula (VII) to generate a compound with a formula (VIII); carrying out dehydration cyclization on the compound with the formula (VIII) to generate a compound with a formula (IX); carrying out condensation on the compound with the formula (IX) to generate a compound with a formula (X); carrying out five-membered ring breakage on the compound with the formula (X) to generate a compound with a formula (XI); carrying out condensation on the compound with the formula (XI) to generate a compound with a formula (XII); carrying out coupling on the compound with the formula (XII) to generate a compound with a formula (XIII); carrying out esterolysis on the compound with the formula (XIII) to generate a compound with a formula (XIV), namely methyl pigeon pea ketonic acid A.

The invention discloses a preparation method of an electrochemical diethylstilbestrol sensor based on a titanium-dioxide-based two-dimensional composite nanomaterial and belongs to the technical field of novel nano functional materials and biosensors. A novel two-dimensional flaky magnetic nano light-sensitive material FeCo-N@TiO2 is prepared firstly; through good bio-compatibility, the large specific area and ferromagnetism of the material, a diethylstilbestrol antibody is loaded, then horse radish peroxidase is fixed through the interlinking effect of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide, electrochemical signals are generated, through the influences of specific quantitative combination of the antibody and an antigen on electron transmission capacity, the current intensity is correspondingly reduced, and finally the electrochemical biosensor which is low in cost, high in sensitivity, good in specificity, rapid in detection and easy to prepare and detects diethylstilbestrol is prepared.

The invention discloses an immunochromatographic test paper for detecting diethylstilbestrol and a preparation method thereof. The immunochromatographic test paper for detecting diethylstilbestrol provided in the invention comprises a sample pad containing superparamagnetic composite particle marked antibodies to diethylstilbestrol, a cellulose nitrate membrane connected with one end of the pad and a water absorbing pad connected with another end of the cellulose nitrate membrane, wherein, the cellulose nitrate membrane is coated with a detection line and a quality control line which are mutually separated, the detection line contains diethylstilbestrol antigen, and the quality control line contains antiantibodies capable of specific binding to the antibodies to diethylstilbestrol. Experiments in the invention indicate that the test paper provided in the invention has the characteristics of high sensitivity, strong specificity, rapidness, simpleness and capacity of realizing objective detection in detecting diethylstilbestrol.

The invention is a kind of immune colloidal gold reagent for measuring the ethene estraphenol and the manufacturing method. The reagent includes a sample cushion, a colloidal gold combination cushion, aeotic acid cellulose film, a suction cushion and a PVC back lining, one end of the PVC back lining is adhered with sample cushion, colloidal gold combination cushion in order, the middle part is adhered with aeotic acid cellulose film, the other end is adhered with a suction cushion. The character lies in: the colloidal gold combination cushion is enclosed with a kind of ethene estraphenol resisting specificity single clone antibody-colloidal gold label, the aeotic acid cellulose film is enclosed by ethene estraphenol-BSA couplings and sheep (rabbit) antiplague IgG. The reagent has a strong specificity, high sensitivity, quick and simple, cheap, and convenient.

The invention discloses a diethylstilbestrol compound and application thereof in preparation of a drug for treating osteoporosis and/or hyperlipemia. The structural formula of the diethylstilbestrol compound is shown in the specification. The data of a cellular level pharmacodynamic test show that cajanstilbene H (R is H, and X is Cl) can promote multiplication and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) of an SD rat. After an ob/ob obese mice orally takes the cajanstilbene H (20 mg/kg/d) continuously for 21 days, the results are as follows: (1) no obvious effect on weight is generated; (2) the bone density of the mice is obviously increased; serum alkaline phosphatase (ALP) and tartrate resistant acid phosphatase (TRACP) level are obviously reduced; and the serum calcium concentration is improved; and (3) levels of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) in the serum of the mice are obviously reduced. The results show that the cajanstilbene H has positive significance in treatment of the osteoporosis caused by obesity and high blood sugar, and has a clear curative effect on the aspect of improvement of lipid metabolism of the obese mice. The cajanstilbene H has a clear treatment effect on the osteoporosis caused by obesity and high blood sugar and simultaneously can improve lipid metabolism; and metabasis of hyperlipemia to diabetes mellitus to chronic complication of diabetes mellitus to osteoporosis can be avoided to a certain extent.

The invention provides a fermentation control method which comprises the following steps: placing saccharified wheat juice into a corresponding yeast growing tank; after yeast proliferation, delivering raw material solution into a can body for further fermentation; when the fermentation temperature rises, guiding cooling alcoholic solution into a cooling jacket; when the fermentation degree of the appearance is 60%, closing a jacket cooling agent valve and raising the temperature naturally for bi-diethylstilbestrol reduction; after temperature rise, starting to seal a can for boosting and then fermenting; when the fermentation degree of the appearance is 65%, raising the temperature according to the preset rate; eliminating yeast for the first time and maintaining a certain pressure and temperature; emptying the yeast and filtering, so as to complete beer fermentation, wherein in the fermentation process, a temperature control module and a pressure control module are used for controlling temperature and pressure. The method provided by the invention is automatically controlled by a computer, the control method is flexible, the quality of beer is improved, the fermentation period is shortened, the system is stable and reliable, and the maintenance management is convenient.

The invention discloses a preparation method of a functional nanomaterial based biological immunosensor with dual signal sending strategies of electrochemiluminescence and photoelectrochemistry.The sensor prepared according to the method has the advantages of simplicity in operation, portability, quickness in detection and low in cost and can be used for rapid and sensitive detection of diethylstilbestrol in fields of daily production, daily life and the like.

The invention relates to the field of environment treatment using microbes, and concretely relates to an Aspergillus niger for metabolizing diethylstilbestrol. The Aspergillus niger for metabolizing diethylstilbestrol is Aspergillus niger YAT, and the Aspergillus niger YAT is registered and preserved in China General Microbiological Culture Collection Center (CGMCC) on Feb. 11, 2015 with the preservation number of CGMCC No.10568. The Aspergillus niger can effectively degrade 20mg/L of diethylstilbestrol, and can be processed to develop corresponding environmentally friendly biological preparations, provides important resources for degradation or removal of diethylstibestrol residual in environment and foods, and has a good application prospect.

The invention relates to a concrete release agent comprising the following components in percentage by mass: 10%-15% of perfluorinated alkyl ethylene oxide, 10%-25% of fatty alcohol-polyoxyethylene ether, 12%-19% of a complexing agent, 14%-20% of n-butyl acetate, 6%-12% of formic acid, 8%-16% of methylphenol, 9%-15% of sodium metasilicate, 3%-8% of diethylstilbestrol, 6%-14% of acetone, 9%-15% of cyclohexanone, 6%-15% of potassium hydroxide, 3%-17% of etidronic acid, 40%-60% of pure water, and 8%-10% of a tribromosalicylaniline, triethanolamine and glycerinum mixture, wherein the weight ratio of tribromosalicylaniline to triethanolamine to glycerinum in the mixture is 2 to 7 to 1. The concrete release agent disclosed by the invention is capable of playing roles in protecting a template, improving the appearance quality of concrete, enhancing the surface smoothness of the film, and the like.

The invention discloses a preparation method of modified pollen and the application thereof for absorbing and treating EDCs of water, which belong to a treatment method for sewage or wastewater. The preparation method disclosed by the invention comprises the following steps: washing natural pollen with ethanol; carrying out solid-liquid separation, drying and crushing; and adding the crushed product into a concentrated hydrochloric acid, sequentially carrying out ultrasonic dispersion and backflow filtering, washing to neutral, and drying, wherein the natural pollen is lotus pollen, piny flower pollen, apricot flower pollen and dandelion pollen. According to the application of the modified pollen for treating EDCs of waer, the modified pollen is added into water containing pollutants such as bisphenol A, estrone, 17beta-estradiol, estriol, progesterone, 17-alpha-ethinyl estradiol, 4-n-nonyl phenol, 4-t-octyl phenol, diethylstilbestrol or tetrabromobisphenol A. The pollen disclosed by the invention is small in polarity, hydrophobic, high in adsorbent active site and saturated adsorption amount, small in desorption ratio, renewable and reusable, therefore, the application is a way of treating water pollutions by using biomass materials.

The invention relates to an estradiol, estriol and diethylstilbestrol three-joint detection card and a processing method of a sample detected by the same, belonging to the technical field of residue detection of estrogen veterinary drugs. A test strip is arranged in a housing of the three-joint detection card, a sample pad, a colloidal gold film, a nitrocellulose film and a water absorbing film are sequentially pasted on a supporting back plate, wherein the colloidal gold film is a glass fiber film containing an estradiol antibody, an estriol antibody and a diethylstilbestrol antibody colloidal gold marker, three detection strips are arranged on the nitrocellulose film and respectively comprise an estradiol protein coupling matter, an estriol protein coupling matter and a diethylstilbestrol protein coupling matter, and in addition, a quality controlling belt containing a rabbit antibody or mat antibody is arranged on the nitrocellulose film. The estradiol, estriol and diethylstilbestrol three-joint detection card can simultaneously detect estradiol, estriol and diethylstilbestrol contained in urine or feeds as well as animal tissues, meat and livers, and has the advantages of easiness to preparation, detection expense saving, convenience and quickness in use and accurate result.