169 results about "Furazolidone" patented technology

A detection method simultaneously detects residue of multiple synthetic antibacterial agents in aquatic products by a high performance liquid chromatography. The synthetic antibacterial agents mainly comprise sulfadiazine SD, sulfamerazine SMR, sulfadimidine SDD, sulfadimethoxine SDM, furazolidone FZD, oxolinic acid OXA, nalidixic acid NAA and the like. The multiple synthetic antibacterial agents in an aquatic product sample are extracted by acetonitrile, degreased by normal hexane, concentrated, purified by a solid phase extraction column (alumina neutral) or a C18 solid phase extraction column, analyzed by a liquid chromatograph, detected by an ultraviolet detector, and quantified by an external standard method. The method can quickly, sensitively and accurately detect multiple target compounds to greatly reduce detection cost and shorten detection time.

The invention discloses a Chinese and western compound medicine for treating stomach diseases, which comprises seventeen traditional Chinese medicinal materials including pilose asiabell root, white atractylodes rhizome, Poria cocos, chicken's gizzard-skin, astragalus root, licorice root, haw, medicated leaven and malt, and a small number of Western medicinal materials including chloramphenicol, terramicin and furazolidone.

The invention discloses a method for simultaneously detecting the medicaments such as furazolidone, diazepam, trimethoprim, acetaminophen and ibuprofen in water. The method comprises the following steps of: filtering a water sample to remove suspended matter; adjusting the pH of the water sample to be 2 to 4; activating hydrophile-lipophile balance (HLB) solid phase extraction column by using acetone, methanol, ammonium acetate-containing formic acid aqueous solution and ultrapure water in sequence; after the enrichment is finished, drying the HLB solid phase extraction column under the protection of nitrogen and eluting the HLB solid phase extraction column; collecting eluant, and drying the eluant by blowing a nitrogen flow; adding acetonitrile to dissolve the residue; and quantitatively detecting the concentration of the five medicaments. By the method, the water sample pretreatment is environment-friendly, easy to operate, large in enrichment factor, and high in reproducibility; and the content of the five common medicaments in a water environment can be analyzed quickly and accurately.

The invention discloses a reagent kit for the chemiluminescence enzyme-linked immunoassay of furazolidone, which comprises a kit body, an enzyme label plate arranged in the kit body and a reagent arranged in the kit body, wherein, holes of the enzyme label plate are coated by an envelope antigen which is manufactured through coupling between 3-azyl-2-oxazolone that is a metabolin of furazolidone and ovalbumin; and the reagent contains a polyclonal antibody of furazolidone, an enzyme label secondary antibody that is a goat anti-rabbit antibody marked by horseradish peroxidase, a furazolidone-series standard solution, a concentrated phosphate buffer solution, a concentrated cleaning solution and a luminescent liquid. The reagent kit has the characteristics of high sensitivity, good reproducibility, simplicity, convenience, speediness and accuracy; compared with the traditional color comparison ELISA method, the sensitivity can be improved by one order higher, and the reagent is expected to play an important role in furazolidone residue detection of water used in stock raising, feeds and animal-derived foods (such as milk samples, animal tissue samples and urine samples).

The invention relates to a fluorescent immunochromatography test strip for detecting furazolidone metabolites as well as preparation and an application of the test strip. The fluorescent immunochromatography test strip comprises a bottom plate, a sample pad, a bonding pad, a chromatography membrane and a water absorption pad, wherein a detection line and a quality control line are arranged on the chromatography membrane; a fluorescent probe of a fluorescent nanoparticle marked AOZ (3-amina-2-oxazolldinone) monoclonal antibody is fixed on the bonding pad; the detecting line is coated with an antigen AOZ-BSA, and the quality control line is coated with a sheep anti-mouse IgG antibody. Compared with the prior art, according to the characteristic of high sensitivity of an amino-modified fluorescent CdTe quantum dot, the fluorescent probe of the marked AOZ monoclonal antibody is prepared, the AOZ fluorescent immunochromatography test strip is prepared and can be used for performing qualitative detection, semi-quantitative detection and auxiliary quantitative detection for a fluorescent reading instrument, so that the detection sensitivity is improved greatly, and the test strip is suitable for AOZ on-site detection and provides higher-value reference for detection of other pesticide residues and antibiotics.

A medicine for treating the intractable tinea, including psoriasis and syphilis, is prepared from 17 raw materials including VB1, VB2, VB6, chlorpheniramine, furazolidone, dexamethasone acetate, etc.

The invention discloses a protein chip for detecting multiple veterinary drug residues and a kit thereof. The protein chip comprises a substrate and dot coatings of veterinary drug antibody, wherein the dot coatings are distributed in an array type, the substrate is a glass substrate or a membrane substrate; and the dot coatings of the veterinary drug antibody refer to eight types of veterinary drug antibodies, i.e. an anti-chloramphenicol antibody, an anti-chlorpromazine antibody, an anti-colchicin antibody, an anti-dapsone antibody, an anti-stilboestrol antibody, an anti-dimetridazole antibody, an anti-furazolidone antibody and an anti-metronidazole antibody which are uniformly distributed and in dot lattice on the substrate. The protein chip can obtain the reaction results with multiple indexes by only one-step reaction and can be suitable for simultaneously detecting eight veterinary drugs, i.e. chloramphenicol, chlorpromazine, colchicin, dapsone, stilboestrol, dimetridazole, furazolidone and metronidazole which are contained in a sample, thereby ensuring the safety and the sanitation of a large quantity of meat products.

The invention relates to application of a microorganism material in detection of environmental pollutants, in particular to a fluorescent iron carrier secreted by pseudomonas aeruginosa (Pseudomonas aeruginosa) PA1 and application thereof. The pseudomonas aeruginosa PA1 is registered and preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation date is April, 28th, 2011; and the number is CGMCC No.4799. When the fluorescent iron carrier secreted by pseudomonas aeruginosa (Pseudomonas aeruginosa)PA1, related by the invention, is used for detecting furazolidone in the environment, the fluorescent iron carrier has the advantages of high specificity and fastness, and the furazolidone is quickly and sensitively detected on the basis of optimization of experimental conditions.

The invention utilizes a surface-enhanced raman spectroscopy to detect antibacterial drugs of furazolidone and furacilin. A method which adopts potassium chloroaurate to reduce sodium citrate is used for preparing aurosol; an acetone liquid which contains the furazolidone or the furacilin is mixed with the aerosol, the pH is regulated to 1.0-6.0, and a laser raman spectrometer with a laser light source of 785 nanometers is adopted to scan, so that a raman spectroscopy is obtained; 1611, 1560, 1488, 1472, 1397, 1343, 1260, 1173, 1022, 977, 806 and 775 centimeters <-1> are used as qualitative characteristic peaks for identifying the furazolidone, and 1602, 1554, 1456, 1336, 1162 and 966 centimeters <-l> are used as the qualitative characteristic peaks for identifying the furacilin; and moreover, the peaks of 1611centimeters <-1> and 1602 centimeters <-1> are respectively used for quantitatively determining the furazolidone and the furacilin. The method is convenient, quick, accurate and low in cost, and can be popularized and used in the fields food and drug testing.

The invention provides a specific beriberi medicament, which is mainly prepared from the following raw materials in portion by weight: 3 to 12 portions of calcined alum, 1 to 4 portions of almond, 0.25 to 1 portion of metronidazole tablet, 0.15 to 1 portion of nystatin tablet, 0.15 to 1 portion of furazolidone tablet, 0.25 to 1 portion of oxytetracycline tablet, 0.15 to 1 portion of vitamin B1 tablet, 0.25 to 1 portion of vitamin B2 tablet, and 10 to 30 portions of clotrimazole cream. The medicament takes antibacterial medicaments as main ingredients, and can kill bacteria and fungi which cause the beriberi; the adding of vitamin B complex and vitamin C aims to repair wound surfaces, promote the healing, and ensure that the skin restores to normal more quickly; the calcined alum ensures that medicament application positions keep relatively dry; the almond relieves internal heat; various components supplement each other, can quickly and effectively treat the beriberi, and enables patients to recover; and at the same time, the medicament also has better efficacies in treating sole sweating and foot odor.

The invention discloses a preparation method and application of a derived hapten and an artificial antigen of a furazolidone metabolite AOZ. An active arm introduced into the hapten completely retains a feature structure of an AOZ derivative and has no influence on electron cloud density of the AOZ derivative; in addition, the active arm has an active group which can be coupled with carrier protein and is mild in reaction conditions and simple in operation step; the prepared artificial antigen can be specifically combined with an antibody, has the characteristics of high titer, high sensitivity, high accuracy and the like, and can be used for quickly detecting residues of the furazolidone metabolites in various products.

The invention belongs to the technical field of Chinese and western medicinal compounds, and in particular relates to a Chinese and western medicinal composition for treating empyrosis, which comprises the following Chinese medicaments or western medicaments in part by weight: 80 to 120 parts of golden thread, 800 to 1,200 parts of medicinal charcoal, 8 to 12 parts of borneol, 80 to 120 parts of elephant's hide, 60 to 90 parts of chloramphenicol, 3 to 7 parts of vitamin B2, 80 to 120 parts of furazolidone, 230 to 270 parts of oxytetracycline and 30 to 70 parts of griseofulvin. The Chinese and western medicinal composition can stop pain quickly, clear away heat and toxic materials, eliminate stasis to subdue swelling, remove slough and promote the growth of tissue regeneration and repair tissue epithelium quickly, has high cure rate and low cost (the cost is 1/20 of regular hospitals), and is not limited by prophetical environment during treatment; and particularly, the Chinese and western medicinal composition has the unique advantage on the aspect of treating diabetes empyrosis patients, namely the blood sugar is not needed to be controlled, the patients are bound up by thickening, the healing time is shortened greatly, and the diabetes empyrosis patients can be basically synchronous with normal empyrosis patients.

The invention relates to 60Cogamma-ray irradiation for degrading five fish drugs of chloramphenicol, sulphadimidine, terramycin, oxolinic acid and furazolidone. In the invention, a water solution containing the five fish drugs generates chemical reaction through the irradiation to generate free radicals of .OH with extremely strong oxidability, hydrated electron of eaq- with extremely strong reducibility, and .H, wherein among the free radicals, the .OH free radical can generate addition reaction with an aromatic series compound or unsaturated groups, the hydrated electron of eaq- can utilizes the low-power empty orbit contained by the reactant aromatic series group to be quickly added on the groups, and the .H free radical can be added on the groups after the reactants are subjected to dehalogenation reaction, thereby causing the degradation of the five fish drugs. The invention has relatively obvious effect on the five fish drugs, wherein the larger the dosage of the absorbent is, the higher the degradation rate of the drugs is; the lower the initial concentration is, the more beneficial to removing the residual drugs; alkalinity, acidity and the deaeration treatment have different influences on the degradation rate of different drugs; and the low-temperature treatment can obviously inhibit the degradation of the drugs.

The invention discloses a drug for curing dysentery, which is produced by uniformly mixing the active pharmaceutical ingredients with the parts by weight: 625 of oxytetracycline, 625 of chloramphenicol, 1000 of sulfamethoxazole, 500 of furazolidone, 200 of methylbenzylamine pyrimidine, 50 of extractum belladonnae, 2500 of sodium bicarbonate and 25 of prednisone acetate to produce into powder, tablet or capsule and other formulation. The drug for curing dysentery has the advantages that the effective rate for curing the dysentery is 100%, the cure rate is 99.99%, and the sufferer can be recovered between 24 to 48 hours; in addition, the drug also has the advantages of fast therapeutic effect, short treatment cycle and low treatment cost.

The invention discloses an enzyme immune detecting agent box suit for analyzing the surplus furazolidone and its application in the field of chemo immunology analyzing technology. The agent box is mainly formed by a 3-amido-2-AOZ peculiarity antibody, a AOZ standard solution which comprises AOZ and the ferment standard plate of the egg albumin complex. The sample releases AOZ after alcaine hydrolysis, then the benzaldehyde derivatizes over the night, MAX column purifies, it adopts indirect contest ELISA method to detect the animal structure such as liver and the AOZ surplus in muscle. The core technology comprises: artificial antibody synthesis, antibody preparing, ELISA method establishing, and ELISA agent box adjustment.

The invention belongs to the technical field of veterinary drug residue analysis and immuno-analysis, and specifically relates to a monoclonal antibody for detecting furazolidone metabolites, an ELISA method, and a kit. The invention mainly comprises the following steps: synthesizing semiantigen, immunogen, and coating antigen, preparing a monoclonal antibody, and establishing an ELISA method. The provided kit is mainly composed of an monoclonal antibody for preventing furazolidone metabolites (AOZ), horse radish peroxidase labeled goat-anti-mouse IgG, and an ELISA plate, in which a coating antigen specifically bonded with the AOZ monoclonal antibody is coated. The provided ELISA kit has the advantages of high sensitivity and high performance/price ratio and can be used to detect the residues of furazolidone metabolites (AOZ) in aquatic products.

The invention discloses furazolidone metabolite hapten and antigen as well as a preparation method and application thereof. The furazolidone metabolite hapten provided by the invention is the compoundshown in the formula (I), and the furazolidone metabolite antigen can be obtained by connecting the compound shown in the formula (I) and carrier protein. The furazolidone metabolite antigen can be applied to preparing the specific antibody of the furazolidone metabolite. The method for preparing the compound is simple in operation and the compound is high in purity and yield. The specific antibody with high affinity for the furazolidone metabolite is generated after the compound of the invention is used for immunizing animals, and enzyme-linked immunosorbent assay established by using the antibody can be used for detecting the residual furazolidone metabolite in the animal derived foods in a rapid, sensitive and simple way. The invention has great economic value and social meaning.

The invention relates to a feed additive for preventing animal diseases, which consists of the following components in weight percentage: 10 to 30 percent of honeysuckle vine, 5 to 15 percent of dayflower, 5 to 15 percent of lophatherum, 5 to 15 percent of glabrous greenbrier rhizome, 7 to 21 percent of small grass, 5 to 15 percent of radix isatidis, 7 to 21 percent of plaster stone and 7 to 21 percent of furazolidone powder, which are mixed. The additive uses suitable Chinese and Western medicaments, can effectively inhibit or kill harmful pathogen in vivo and regulate normal physiological metabolism, can further remove the harmful pathogenic fungi in vivo, maintain the normal physiological metabolism, improve the immunity of a colony and maintain favorable colony health for a normal individual, so as to achieve the aim of preventing diseases.

The method of temporarily keeping river crab for sale includes four parts, including selecting temporarily keeping pond, selecting river crab to be kept, determining temporarily keeping density and managing. It features the selection of temporarily keeping pond near water source with convenient water pouring and draining ports, area of 3-5 unit of area and water depth of 1.5-1.8 m; selecting robust river crab and sterilizing with 4 wt% concentration salt solution for 10-15 min; the temporarily keeping density of 2000-2500/mou or 200-250 kg/unit of area and temporarily keeping period of 1-3 months; and managing including laying bait of 3-5 wt% of crab weight once a day, changing water of 15-25 cm once every 5-7 days, spraying lime once every 20-30 days, spraying 0.5 ppm concentration trichloroiso cyanuric acid concentration to sterilize water regularly, and feeding terramycin and furazolidone mixed into feed for continuous 5 days every half one month.

The invention discloses an oil-in-water furazolidone nano emulsion antibacterial medicament. The diameter of the nano emulsion drops is between 10 and 100 nanometers; and the medicament is prepared from the following raw materials in percentage by mass: 20.00 to 40.00 percent of surfactant, 2.00 to 16.00 percent of cosurfactant, 1.00 to 5.00 percent of oil, 0.01 to 2.00 percent of furazolidone, 1.00 to 12.00 percent of auxiliary material and the balance of distilled water, wherein the mass percentage sum of the raw materials is 100 percent. According to the medicament, the dissolubility of the furazolidone is enhanced, the stability of the medicament is improved, the bioavailability of the furazolidone is improved, the medicament metabolizing time of the furazolidone in vivo is delayed, the consumption of the auxiliary material is reduced, and the production cost is lowered; and the medicament has broad market prospect in the field of medicine.

The invention relates to an external coating ointment for burns and scalds. The external coating ointment is prepared from the following medicines by using the following preparation methods: (1) preparing the following raw materials: 15g of furazolidone tablets, 3500 units of neomycin sulfate, 10g of a fluocinonide ointment, 5g of sesame oil, 15g of snake slough, 15g of astragalus membranaceus, 15g of radix stemonae, 15g of radix sophorae flavescentis and 10g of human urine agglomerate; (2) baking the snake slough, and decocting and concentrating the snake slough, the astragalus membranaceus, the radix stemonae and the radix sophorae flavescentis with a proper amount of water to obtain a thick medicine liquid; (3) mixing and crushing the human urine agglomerate, the furazolidone tablets and the neomycin sulfate into mixed medicine powder; (4) mixing the mixed medicine powder with the thick medicine liquid, the fluocinonide ointment and the sesame oil into mixed cream; and (5) enclosing the mixed cream into a glass bottle or an ointment shell and sealing. According to the external coating ointment for burns and scalds, the traditional Chinese medicines and the western medicines with the effects of efficiently killing insects, sterilizing, removing wind and expelling toxin are combined; a specific medicine human urine agglomerate is utilized to prepare the ointment; the ointment can be timely coated by a patient, and is capable of quickening wound healing, preventing and inhibiting tumor growth of the wound, inhibiting hyperblastosis, and healing and smoothing the wound.

The invention relates to a herbal shampoo for effective anti-Schuppen, hair heath, hair growth and hait protection, which can solve problem of scurf, hair-tickle, hair-dry and hair-lose for vast consumers. The formula includes sodium dodecyl sulfate, octadecanol, magnesium stearate, borax, glycerine, ammonia chloride, essence, sublimed sulfur, vitamin E, borneol, polygonum multiflorum, angelica, eclipta prostrata, Glossy Privet Fruit, aloe, Densefruit Pittany Root-bark, Radix Stemonae, phellodendron, rhizoma ligustici wallichii, deionized water, DMSO nystatin and furazolidone, etc,.

The invention relates to a colloidal gold immunochromatography test strip for detecting furazolidone metabolites and a preparation method and application of the colloidal gold immunochromatography test strip, and belongs to the technical fields of immunology and veterinary drug residue analysis. The test strip comprises a base plate, a sample pad, a colloidal gold pad, a coating membrane and a water absorption pad; protective membranes are arranged at the two ends of the test strip, and the sample pad, the colloidal gold pad, the coating membrane and the water absorption pad are sequentially stuck to the base plate; the colloidal gold pad is coated with colloidal gold-labeled monoclonal antibodies which can recognize the furazolidone metabolites; the coating membrane is provided with an invisible detection line printed by a carrier protein solution coupled with the furazolidone metabolites and an invisible quality control line printed by a goat anti-mouse IgG solution. The colloidal gold immunochromatography test strip has the advantages of being convenient and rapid to operate, visual, high in timeliness and the like and is wide in application range, low in cost and convenient to apply and popularize.

The present invention relates to a monoclonal antibody and applications thereof, especially to a furazolidone metabolite derivative monoclonal antibody and applications thereof, and belongs to the technical field of immunochemistry. The furazolidone metabolite derivative monoclonal antibody 1C3' is produced by mouse hybridoma cell line 1C3, wherein a subtype is IgG1 type. According to the present invention, the monoclonal antibody 1C3' produced by the mouse hybridoma cell line 1C3 can be abundantly produced, and can be used to prepare an enzyme linked immunoassay kit and colloidal gold test paper strips for furazolidone metabolite (AOZ) detection so as to achieve a purpose of rapid and sensitive detection of furazolidone drug metabolites in muscle, liver and aquatic products and establish a foundation for furazolidone metabolite residue detection kit and colloidal gold product development.

The invention provides a kit and a method for detecting and determining the drug resistance of helicobacter pylori. The method comprises the following steps: adopting nine pairs of primers having nucleotide sequences as shown in SEQ ID NO. 1-18 to detect mutations in 41 loci including six genotypes: 23sRNA, PBP1, PORD, OORD, 16s rRNA, GYRA and rdxA; comprehensively analyzing mutation loci of six existing mainstream helicobacter pylori drug-resistant genes; and finding corresponding genotypes according to the loci, thereby judging the resistance to clarithromycin, amoxicillin, furazolidone, tetracycline, levofloxacin and metronidazole. The method has a sequencing read length up to 1000bp, an accuracy up to 99.999%, and great significance for guiding personalized treatment for patients withhelicobacter pylori.