50 results about "Lipid raft" patented technology

In this application are described filovirus-like particles for both Ebola and Marburg and their use as a diagnostic and therapeutic agent as well as a filovirus vaccine. Also described is the association of Ebola and Marburg with lipid rafts during assembly and budding, and the requirement of functional rafts for entry of filoviruses into cells.

The present invention relates to the use of agents that inhibit the toxic effects of amyloid oligomers by increasing intracellular levels of phosphoinositol 4-phosphate (PI(4)P, or “PIP”) and / or phosphotidylinositol 4,5-biphosphate (PI(4,5)P2 or “PIP2”), the use of such agents for the treatment of neurodegenerative diseases, methods of treating neurodegenerative diseases by administration of agents which alter lipid metabolism, and methods of identifying agents which alter the association of presenilins with γ-secretase and lipid rafts.

The invention discloses a chromatographic column decorated by lipid rafts. The chromatographic column is a silica gel chromatographic column which is decorated with the lipid rafts on the surface. A TrkA lipid raft-sillica gel stationary phase chromatographic system is built firstly. Stationary phase can be combined with medicinal specificity of tyrosine kinase receptors as a target spot, and then reservation is produced, but non-reservation behavior is not existed in the TrkA lipid raft-sillica gel chromatographic column of the tyrosine kinase target spot medicines. Lipid raft-sillica gel chromatograph which is rich in TrkA receptors is a novel medicine screening model which has a living being affiliation character and is capable of selective recognition medicines, wherein the medicines are reacted with the TrkA receptors. The chromatographic system is different from the traditional chromatographic system and has double characters of chromatograph separation and activity recognition. Therefore, the chromatographic system can be used for guide medicine screening study where the TrkA receptors served as drones. The chromatographic column decorated by the lipid rafts is used through different time and different moving phases, and the TrkA lipid raft-sillica gel stationary phase chromatographic system is proved stable. The chromatographic column is good in repeatability, long in service life and has good application prospects. The invention discloses a manufacturing method of the chromatographic column decorated by the lipid rafts.

Methods of reducing the risk of transmission of a sexually transmitted pathogen by contacting the pathogen or cells susceptible to infection by the pathogen with a β-cyclodextrin are provided. Methods for reducing the risk of transmission of a sexually transmitted pathogen to or from a subject by contacting the pathogen or cells susceptible to the pathogen in the subject with a pharmaceutical composition containing a β-cyclodextrin also are provided. Accordingly, pharmaceutical compositions, which include 1) a β-cyclodextrin, which is in an amount that blocks passage of the pathogen through lipid rafts in the membrane of a cell susceptible to the pathogen, and 2) a contraceptive, an agent for treating a sexually transmitted disease, a lubricant, or a combination thereof, are provided, as are composition formulated from a solid substrate that contains an amount of β-cyclodextrin useful for reducing the risk of transmission of a sexually transmitted pathogen.

Two-photon dyes for real-time imaging of lipid rafts are provided. The two-photon dyes emit fluorescence with high intensity in the environments of cell membranes and can be used to clearly discriminate the hydrophobic and hydrophilic domains of the cell membranes. Therefore, the use of the two-photon dyes enables satisfactory imaging of actual lipid raft domains. In addition, since the two-photon dyes show no cytotoxicity, they can be suitably used in bioimaging applications, for example, real-time imaging of lipid rafts over a long period of time.

In this application are described filovirus-like particles for both Ebola and Marburg and their use as a diagnostic and therapeutic agent as well as a filovirus vaccine. Also described is the association of Ebola and Marburs with lipid rafts during assembly and budding, and the requirement of functional rafts for entry of filoviruses into cells.

The present invention relates to the application of human recombined protein PDCD5 in preparing tumor chemotherapy temoporfin. Human recombined protein PDCD5 is non-virus intracellular transfer protein, which enters into cells through lipid raft / caveolae-dependent phagocytosis; PDCD5 can promote the death of programmed cells; the enter of the exogenous human recombined protein PDCD5 into cells can strengthen the sensitivity of various tumor cells to various chemotherapeutic drugs and promote the death of tumor cells caused by chemotherapeutic drugs.

The invention discloses a tumor detection kit adopting a lamprey Li proteins as a marker. The kit contains a capture reagent and a detection reagent, and the capture reagent and the detection reagentare recombinant lamprey Li proteins capable of specifically marking sugar and sphingomyelin of lipid rafts on the surfaces of tumor cells. The above prepared recombinant protein rLi protein has higherstability, specificity and affinity than antibody molecules, and still keeps complete antigen binding capability and is more sensitive to tumor cell antigens after being stored at 4 DEG C for one year or more when in a dissolved state. The kit has the advantages of high detection sensitivity, convenience in storage and transportation, long validity and low production cost.

The invention relates to the technical field of biological pharmaceuticals, in particular to a fusion protein and an application thereof. According to the fusion protein, a plasma membrane positioningsequence, a Dab1 protein, a light-sensitive protein and a fluorescent protein are in fusion to be positioned to a lipid raft region or a non lipid raft region of a cytoplasmic membrane, and through illumination, quick activation and adjustment and control of a PI3K / Akt signal channel can be realized locally at a high time and space resolution. The fusion protein has great important significance in quick effective specific opening and closing control of the signal channel, contributing to understanding a cascade transmission rule of signals and researching diseases closely related to the signal channel.

A biological composition has a mixture of mechanically selected allogeneic biologic material derived from placental tissue. The mixture has non-whole cellular components including vesicular components and active and inactive components of biological activity, cell fragments, cellular excretions, cellular derivatives, and extracellular components. The mixture including non-whole cell fractions including one or more of exosomes, transcriptosomes, proteasomes, membrane rafts, lipid rafts. The mixture is compatible with biologic function.

Two-photon dyes for real-time imaging of lipid rafts are provided. The two-photon dyes emit fluorescence with high intensity in the environments of cell membranes and can be used to clearly discriminate the hydrophobic and hydrophilic domains of the cell membranes. Therefore, the use of the two-photon dyes enables satisfactory imaging of actual lipid raft domains. In addition, since the two-photon dyes show no cytotoxicity, they can be suitably used in bioimaging applications, for example, real-time imaging of lipid rafts over a long period of time.

Immunoreactive compositions and methods for immunizing animal, including, humans, cows, and fowl, against cholesterol and cholesterol derivatives, including cholesterol oxides, and their use in methods for reducing and preventing lipid raft-based diseases, including, but not limited to HIV-1, SARS, prion formation in Creutzfeldt-Jakob disease, and neutralizing oxidized modified lipoproteins, specifically, oxidized-LDL, oxidized-VLDL / IDL, and oxidized-chylomicrons, which contribute to the formation of fatty streaks and atherosclerotic plaques, are described.

The present invention discloses methods of using endothelial membrane protein 2 (EMP2) as a biomarker for stratification of endometrial premalignancy, diagnosing, staging and imaging of endometrial neoplasia. Further, methods for identifying pharmaceutical / therapeutic modalities are described, including compositions which modulate glycolipid-enriched lipid raft microdomains (GEMs).

The invention discloses a single fluorescent probe for clearly distinguishing a cell membrane-lipid raft microdomain from a non-lipid-raft microdomain by using two fluorescence colors in a real-time in-situ manner and simultaneously imaging the microdomains. The fluorescent probe is a compound with a structure shown as a formula (I), wherein R1 expresses 2-ethoxyethyl, aminoalkyl, hydroxyalkyl or alkyl; R2 and R3 express alkyl. The invention also discloses application of the fluorescent probe in marking the lipid raft microdomain and the non-lipid-raft microdomain on a cell membrane of a living cell by using red and green fluorescence colors in the real-time in-situ manner. The probe can mark the lipid raft microdomain by using red fluorescence, and marks the non-lipid-raft microdomain by using green fluorescence at the same time, thereby realizing simultaneous imaging of the two microdomains. Compared with a current commonly-used similar probe laurdan, the probe disclosed by the invention has the characteristics that spectrum difference of two microdomains is greater, so that clear distinguishing and simultaneous imaging of the two microdomains are realized; besides, the internal problems is improved, so the probe is simpler and more convenient to operate during application and is broad in commercial application prospect.

The invention discloses a dual-channel structured light illumination super-resolution imaging method and device, which include an ordinary fluorescence microscope, and also include a light source, a first reflecting mirror, a second reflecting mirror, a first lens, a diaphragm, a second lens, and a space light Modulator, third lens, light baffle plate, fourth lens, second dichromatic mirror, first bandpass filter, second bandpass filter, first detector and second detector. At the same time, the invention also discloses a microscopic imaging method based on the imaging system. The imaging system and imaging method can measure the heterogeneity of lipid rafts on the cell membrane at the scale of super-spatial resolution.