89 results about "Lamprey" patented technology

The invention discloses a powder compound feed for adult Japanese eels. The feed comprises the following components in percentage by weight: 52.5 to 60.0 percent of steam-dried fish meal, 24.0 to 26.0 percent of pre-gelatinized starch, 7.0 to 11.5 percent of extruded soybean, 0.5 to 1.5 percent of fish oil, 2.0 to 4.0 percent of beer yeast, 1.0 to 2.0 percent of mineral, 0.5 to 1.0 percent of monocalcium phosphate, 0.3 percent of choline chloride and 0.2 percent of multi-vitamin. The feed can supply sufficient nutrition at an adult Japanese eel breeding stage and eel breeding cost is lowered.

The invention relates to an eel breeding pond and belongs to the technical field of breeding equipment. The eel breeding pond comprises a breeding pond body formed by pouring concrete, a cover plate and a water injection connector. The water injection connector is arranged over the culture pond body and used for injecting water into the culture pond body through a water injection pipe. The bottom of the breeding pond body is provided with a first drain outlet in connection with a rear breeding pond of the breeding pond. The inner surface of the bottom of the breeding pond body is provided with a rock ballast layer and a yellow sand layer. An escape prevention net, a first gate and a second gate are sequentially arranged in the rear breeding pond from one side of the first drain outlet. One side of the bottom of the rear breeding pond, which is close to the second gate, is provided with a second drain outlet. A feeding device for feeding eel is also arranged in the breeding pond body. Therefore, the eel breeding pond has the functions of escape prevention, eel catching easiness and water injection and drainage convenience, is easy to feed, can maintain a constant water level in long term, provides a good living environment for eel, and is suitable for popularization and application.

The gene clone and expression of Japonese lampreyí»s oral gland model proteins RGD with antineoplastic action are disclosed. Three cDNA sequences with said model proteins RGD and their clones, the protein sequence and its expression in engineered bacteria, and the action of proteins RGD in closing the signal transfer channel to suppress the vascular neogenesis and tumor cell reproduction for antineoplastic purpose are also disclosed.

The invention discloses a nuisance-free bulked granulated compound feed for adult eels, which comprises steam heated fish meal 45-55%, flour 18-25%, soybean waste 5-15%, bulked soybean 1-10%, fish oil 2-6%, brewer's yeast powder 2-6%, minerals 1-3%, choline chloride 0.3-1%, and vitamins for eels 0.2%. The feed has a higher conversion rate.

The invention discloses a nuisance-free bulked granulated compound feed for eels, which comprises steam heated fish meal 55-63%, flour 20-25%, soybean waste 2%-10%, bulked soybean 1-5%, fish oil 2-4%, brewer's yeast 2-5%, minerals 1-3%, choline chloride 0.3-1%, and vitamins for eels 0.2%. The feed has a higher conversion rate.

The invention discloses a combined tool for processing sea eel or finless eel. The invention aims to provide a combined tool which is convenient and quick in operation, low in cost and fit for killing a small amount of sea eel or finless eel in the places, such as home, restaurants and hotels. The combined tool comprises a sea eel or finless eel automatic loading device, a shaping elastic sleeve and a cutter, wherein the sea eel or finless eel automatic loading device comprises a storage box with an opening at the upper end and a plurality of vertical insert plates which are arranged in the storage box and can be taken out of the storage box; a plurality of loaders are respectively arranged on the vertical insert plates; mesh enclosures are respectively arranged on one side of each of the vertical insert plates; the loaders on the same vertical insert plate are all located in the corresponding mesh enclosures; each of the loaders comprises an inducing sleeve, a pin, a dragging line, a trigger rod, a spring steel sheet and a bait bag; the spring steel sheet is arranged right under the inducing sleeve; the bait bag is arranged in the inducing sleeve; one end of the inducing sleeve is fixed on a side wall of each of the vertical insert plates; penetrating through holes are respectively arranged on the side walls of the vertical insert plates corresponding to the inducing sleeve.

The invention relates to the field of aquaculture, in particular to an indoor high-density breeding method of current-year fingerlings of scatophagus argus. The indoor high-density breeding method consists of breeding preparation, fingerling pulling net counting, fingerling transportation and delivery, bait management, water quality management and pulling net capture and adopts an indoor cement pool for high-density breeding of the current-year fingerlings of the scatophagus argus; aquaculture water is estuary brackish water, artificially-bred fingerlings that are converted to eel feed are transported to the indoor cement pool, the specification body length is 1.3-1.5 cm, and the breeding density is 300-400/m<2>; the eel feed is fed to the fingerlings with a respective body length of lessthan 3 cm, and floating pellet feed is fed to the fingerlings with a respective body length of 3 cm; during breeding, sewage suction and bottom cleaning are performed on time, and water change and pool turnover are performed timely; as for the current-year fingerlings with a respective specification body length of more than 4-5 cm, the pulling net capture can be performed to transfer to the pool for breeding. The indoor high-density breeding method provided by the invention has the beneficial effects that the survival rate after indoor high-density breeding for 181 days is 80-85 percent, and the technical blank of indoor intensive and large-scale production of the current-year fingerlings is filled up.

The invention discloses liproteins, a preparation method and an application of liproteins in preparing a medicament for preventing and treating tumor diseases. The liproteins are pure natural proteins which are separated and purified from a cell culture fluid of lamprey corpuscle, is named Liproteins, has a Jacalin_like domain and a Pore-formingtoxin_like domain, and can be synthesized through expression of a heterologous expression system to form recombinant protein of liproteins. The liproteins have a specific killing function of tumor cells, does not destroy normal cells, and does not have side effect to human bodies.

The invention disclose a granulated eel compound feed which is prepared by puffing the following components and then sieving by a 40-meshed sieve: 42-50% of fishmeal, 10-15% of shrimp powder, 10-15% of corn flour, 10-15% of flour, 10-15% of soybean meal, 5-10% beer yeast, and the balance vitamins and minerals for eel. The eel compound feed of the invention has balanced nutrition, low cost, high transformation efficiency and stable quality, eel can grow well after eating the feed, and the yeast can protect eel from eel viruses, reduce occurrence of diseases, and improve eel yield.

The invention relates to a Scylla serrata seed cultivating pool and relative method, in particular to a method for improving survival rate and growing seed Scylla serrata quickly. the invention comprises a main pool and a seed pool, wherein the bottom of th main pool is provided with a slope inclined along a water outlet, and a sand layer, the sand layer is provided with at least one groove extended from the high part to the lower part of the bottom of the main pool to be collected into the water outlet, the seed pool is arranged under the water outlet with a network box inside. The water specific weight is 1.006-1.015 while the seed cultivating density is 2000-3000n/m2, while the water is exchanged each day and the forge is fish slurry, shellfish meat, or the like to be fed for at least four times and 2-3 times of seed weight, and the whole cultivation is aerated continuously.

Provided is an indoor cement pond culturing method of artificially bred sebastiscus marmoratus fingerling. The method is characterized in that in May to June every year, the specification of artificially bred sebastiscus marmoratus juvenile fishes reaches 2 cm to 4 cm, domestication of salinity and bait is carried out, the salt reducing speed is 2%/d, the final breeding salinity is kept at 11% to 15%, domestication bait of the juvenile fishes is sea eel feed, and more than 90% of the juvenile fishes take mixed feed; when the feed scrambling phenomenon occurs, one-age fingerling is cultured and cultured to November to December, and when the water temperature is below 15 DEG C, sebastiscus marmoratus is transferred into an indoor cement pond for overwintering culture; the water temperature of culture water is raised to 15 DEG C or above in next spring, the sebastiscus marmoratus overwintering fingerling gets into a two-age fingerling culture stage, and the fingerling is cultured till overwintering. The survival rate of the sebastiscus marmoratus can reach 72% to 82%, the overwintering survival rate is 90% or above, operation is easy and convenient, and popularization is easy.

The invention relates to a raising method for promoting quick growth of young loaches. The raising of young loaches is divided into 2 stages which are a 0.3 to 3cm body length stage and a 3 to 5cm body length stage respectively, wherein at the 0.3 to 3cm body length stage, soybean milk and young eel feed, of which the content of crude proteins is 47 percent are mixed in a ratio of 1 to 5 and then used for feeding the young loaches; and at the 3 to 5cm body length stage, the noturus flavipinnis feed of which the content of crude proteins is 32 percent and the snakehead feed of which the content of crude proteins is 44 percent are mixed in a ratio of 2 to 1 and then used for feeding the young loaches. The raising method can promote the quick growth of the young loaches, lower the risk of contracting a disease, and improve survival rate of the young loaches.

The invention discloses a preparation method of an affinity chromatography purification tag-free genetic recombinant lamprey Lj-RGD3 protein rLj-RGD3(Tag<->). The preparation method has simple processes and a high purification rate. According to the preparation method, a recombinant expression vector pET23b-RGD3(Tag<->) without any purification tags is constructed; the recombinant plasmid is transferred into expression bacteria and a genetic recombinant protein is expressed; and through metal-chelating ion affinity chromatography, cation exchange and dialysis, the lamprey Lj-RGD3 genetic recombinant protein without any affinity chromatography purification tags is prepared, is named as rLj-RGD3(Tag<->) protein and has protein purity greater than or equal to 99%.

The invention relates to a method for extracting sea eel small molecular active peptide from sea eel bones. The method comprises the following steps of 1, sea eel bone processing, 2, low-temperature smashing, 3, cell dissolution processing, 4, biological enzymolysis processing and 5 postprocessing; the ratio of the obtained sea eel small molecular peptide with the molecular weight being 0-480 d is 88.5% or more; the sea eel small molecular peptide is applicable to a peptide for diagnosis, a peptide vaccine, an antibacterial activity peptide, a cell factor simulation peptide, a cell factor simulation peptide, an antiviral peptide or an anti-tumor peptide and a peptide health care product.

The invention relates to dimeric proteins comprised of subunits having (i) recombinant lamprey variable lymphocyte receptor (VLR) diversity regions linked to (ii) multimerization domains. The dimeric proteins exhibit binding specificity for glycosylated antigens, and they may be used in methods of detecting or isolating glycans from a sample, and in methods of disease diagnosis, prognosis, progression monitoring, treatment, and imaging.

A gene clone and expression of the oral gland KGD pattern protein of Japanese lamprey with anti-thrombosis is disclosed. Its cDNA sequence and clone, its relative protein sequence, its expression in colibacillus and Piohia yeast, its combination with the glucoprotein gpó�b/IIIa on cell membrane of platelet to suppress the platelet coagulation, and its application in developing the anti-thrombosis medicines are disclosed.

The invention relates to a technology for breeding fish, in particular to a technology for breeding marble goby, which belongs to the technical field of aquiculture. The technology comprises maintenance of the pond, throwing and feeding of breeding baits, and the like. The field pond is used for cultivating hyacinth and narcissus, thereby reducing the content of ammonia nitrogen in pond water and keeping the water quality and the water temperature stable; and fish slurry mixed by iced fresh fish or eels is fed as the bait, thereby accelerating the growth of fries and providing the market with a byproduct which has the advantages of delicious flavor, high safety and reliability.

The invention discloses a technology for cultivating takifugu flavidus summer fingerlings in a cement pond of a simple plastic greenhouse. The cement pond is adopted, and an arch-shaped ring top is formed above the cement pond. The technology is characterized in that: a dimmable shading film covers on the arch-shaped ring top; the air density in the cement pond is 1-1.5 pieces per square meter; the stocking sizes are 10-12 millimeters in full lengths per fingerling; the stocking density is 1,000-1,500 fingerlings per square meter; the water level is 70 centimeters during stocking; 20 centimeters of fresh water is injected every day from the second day, the cement pond is full of water four days later, and 50 percent of water is changed every other day from the fifth day; after 10-15 days of cultivation, bait scraps and fish excrement are cleared; the water quality indexes are that: the salinity of sea water is 0.8-1.2 percent, the water temperature is 25-28 DEG C, the dissolved oxygen is over 5 mg/L, and the pH is 7.5-8.5; medium-sized cladocerans and copepods are fed at an earlier stage, and large frozen fairy shrimps are fed at a middle stage; and after the full lengths of fingerlings are up to 30 millimeters, a compound feed is fed instead of the frozen fairy shrimps, and an eel feed is fed completely after 3-4 days of transition.

The invention discloses a river eel culturing method comprising the following steps of ground processing, preprocessing forage sorting and feeding and daily management. With the above method, pond culturing output can be increased; natural foods are employed for the culture, so product is green and safe and taste can be remained; and river eel quality can be increased and diseases can be prevented.

The invention aims to provide an aquaculture process for South American eel larvae. The aquaculture process includes cleaning aquaculture ponds, and controlling the water temperatures and the salinity of the eel fry ponds. The aquaculture ponds are soaked or cleaned by the aid of dry quicklime powder, bleaching powder, potassium permanganate, clear water and the like; the water temperatures and the salinity of the aquaculture ponds are controlled in such modes that temperature difference of the ground are regulated, water is blended, salt is added into the water at specified steps, the water is diluted at specified steps and the like. The aquaculture process has the advantages that cleaning optimization schemes implemented before the aquaculture ponds are utilized are optimized by means of exploration, and methods for removing cement alkali are improved; aquaculture pond sterilization modes are optimized; the growth speeds of the eel larvae are increased, eel deformity can be prevented, and the death rates in larva aquaculture periods can be decreased; eel larva releasing modes and salinity regulation modes are modified, and accordingly death of the eel larvae due to temperature or salinity problems in releasing procedures further can be reduced.

An ecological cultivation method of fugu flavidus intercropping barracuda parental fish is characterized in that barracudas are released to prepare parental fish in winter; an outdoor earth pond is provided with an aerator of 1.5 Kw at every 3 to 5 mu; the pond is disinfected by quick lime before the barracudas are released; water is injected into the pond a week later and the water source is natural seawater; after ten days of injecting the water, two-year-old barracudas are released to prepare parental fish as backup; the density is within the range of 20 to 30 tails per mu without feeding fish meal; in spring, the water temperature of the outdoor earth pond is risen to 14 to 15 DEG C; two-year-old fugu flavidus fingerlings that live through winter are released into the outdoor earth pond; the density is within the range of 800 to 1000 tails per mu; the fugu flavidus fingerlings are fed with baits twice a day; the daily bait-feeding amount is 3 to 8 percent of the weight of each of the fugu flavidus fingerlings; the baits are eel feeding stuff; the next bait-feeding amount is regulated timely according to the ingestion situation; the water is changed once every two weeks; from mid to late of November, the fugu flavidus fingerlings are started to be caught into a greenhouse for living through winter; and the barracudas are kept in the pond to early January and the pond is dried for catching the barracudas when the temperature is relatively low.

The invention discloses a fish processing product, in particular a smoked eel product. The product is an eel processing product produced by taking fresh eels as a raw material, using a flavoring to cure after the killing, and performing smoking and packaging. The most typical characteristic of the product which is different from other smoked food is that the moisture content is high, the moisture content of a smoked raw eel frozen product is more than or equal to 50 percent, and the moisture content of a smoked cooked eel product is more than or equal to 45 percent, so the disadvantages of fat overflow and excessive weight loss of the eels caused by smoking dehydration can be overcome, the quality of products can be improved, and even the risk of overhigh production cost caused by weight loss can also be reduced.