Sea eel small molecular peptide extracted from sea eel bones and extraction method thereof
A technology of small molecule peptides and small molecule active peptides, applied in the direction of fermentation, etc., can solve the problems of long production cycle, low absorption rate, incomplete removal of impurities in enzymatic hydrolysis solution, etc., and achieve the effect of wide application range and easy preservation
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Embodiment 1
[0019] A method for extracting eel small-molecule active peptides from eel bones, comprising the following steps: (1) eel bone treatment: fresh eel bones are cleaned and crushed, and in a cooking tank, the temperature is 120° C., 0.13 Mpa pressure cooking for 10 hours, bone residues are discharged to obtain bone broth, after quick freezing, frozen bone broth oil jelly blocks are formed, stored in a freezer at -18°C for subsequent use; The frozen bone broth jelly block is crushed at low temperature, and the pulverization temperature is controlled at 4° C., and the frozen bone broth jelly block is pulverized into frozen bone broth jelly block particles with a particle diameter of 0.02 mm; (3) cell dissolution Treatment: Use anaerobic water at 4°C under normal pressure to impregnate the pulverized frozen bone broth jelly block particles, and the water saturation time is 8 hours. The anaerobic water is based on completely submerging the raw materials; Thawing treatment, in which t...
Embodiment 2
[0021] A method for extracting eel small-molecule active peptides from eel bones, comprising the following steps: (1) eel bone treatment: fresh eel bones are cleaned and crushed, and in a cooking tank, the temperature is 120° C., 0.13 Mpa pressure cooking for 10 hours, bone residues are discharged to obtain bone broth, after quick freezing, frozen bone broth oil jelly blocks are formed, stored in a freezer at -18°C for subsequent use; The frozen bone broth jelly block is crushed at low temperature, and the pulverization temperature is controlled at 0° C., and the frozen bone broth jelly block is pulverized into frozen bone broth jelly block particles with a particle diameter of 0.05 mm; (3) cell dissolution Treatment: Use anaerobic water at 4°C under normal pressure to impregnate the pulverized frozen bone broth jelly block particles, and the water saturation time is 8 hours. The anaerobic water is based on completely submerging the raw materials; Thawing treatment, in which t...
Embodiment 3
[0023] A method for extracting eel small-molecule active peptides from eel bones, comprising the following steps: (1) eel bone treatment: clean fresh eel bones, crush them, and use a temperature of 125° C. in a cooking tank at a temperature of 0.14 Mpa pressure cooking 8h, bone dregs are discharged to obtain bone broth, after quick freezing, frozen bone broth oil jelly blocks are formed, stored in a freezer at -18°C for subsequent use; The frozen bone broth jelly block is crushed at a low temperature, and the crushing temperature is controlled at 2° C., and the frozen bone broth jelly block is pulverized into frozen bone broth jelly block particles with a particle diameter of 0.04 mm; (3) cell dissolution Treatment: use 4°C anaerobic water under normal pressure to carry out impregnation water treatment on the crushed frozen bone broth jelly block particles, the water saturation time is 7 hours, and the anaerobic water is subject to completely submerging the raw materials; then ...
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