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Sea eel small molecular peptide extracted from sea eel bones and extraction method thereof

A technology of small molecule peptides and small molecule active peptides, applied in the direction of fermentation, etc., can solve the problems of long production cycle, low absorption rate, incomplete removal of impurities in enzymatic hydrolysis solution, etc., and achieve the effect of wide application range and easy preservation

Inactive Publication Date: 2016-04-20
潘爱国
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current extraction is mainly to extract collagen from fish scales and fish skins, such as tilapia fish scales, deep-sea fish skins such as cod skins, but these extracts have a wide molecular weight distribution and are not easy to absorb. The existence of outstanding problems such as low absorption rate has greatly prevented the realization of new health care concepts such as high nutritional value, safety, and good absorption that modern people pursue.
[0004] On the other hand, the volume of traditional collagen or protein peptide extracts is large, the production cycle of the product is long, and the enzymatic solution is not completely removed, which affects the quality of collagen. The resulting finished product often has a bitter smell and poor taste. If filtered If the level is not enough, the water solubility of the product will be poor, which will directly affect digestion and absorption, and the solution to the problem of high salt content in fish extracts is not ideal, which will affect the taste of the product
[0005] Although with the advancement of technology, people have used the advantages of membrane separation technology to improve product quality to a certain extent, but they have not fundamentally obtained small molecule active peptides.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for extracting eel small-molecule active peptides from eel bones, comprising the following steps: (1) eel bone treatment: fresh eel bones are cleaned and crushed, and in a cooking tank, the temperature is 120° C., 0.13 Mpa pressure cooking for 10 hours, bone residues are discharged to obtain bone broth, after quick freezing, frozen bone broth oil jelly blocks are formed, stored in a freezer at -18°C for subsequent use; The frozen bone broth jelly block is crushed at low temperature, and the pulverization temperature is controlled at 4° C., and the frozen bone broth jelly block is pulverized into frozen bone broth jelly block particles with a particle diameter of 0.02 mm; (3) cell dissolution Treatment: Use anaerobic water at 4°C under normal pressure to impregnate the pulverized frozen bone broth jelly block particles, and the water saturation time is 8 hours. The anaerobic water is based on completely submerging the raw materials; Thawing treatment, in which t...

Embodiment 2

[0021] A method for extracting eel small-molecule active peptides from eel bones, comprising the following steps: (1) eel bone treatment: fresh eel bones are cleaned and crushed, and in a cooking tank, the temperature is 120° C., 0.13 Mpa pressure cooking for 10 hours, bone residues are discharged to obtain bone broth, after quick freezing, frozen bone broth oil jelly blocks are formed, stored in a freezer at -18°C for subsequent use; The frozen bone broth jelly block is crushed at low temperature, and the pulverization temperature is controlled at 0° C., and the frozen bone broth jelly block is pulverized into frozen bone broth jelly block particles with a particle diameter of 0.05 mm; (3) cell dissolution Treatment: Use anaerobic water at 4°C under normal pressure to impregnate the pulverized frozen bone broth jelly block particles, and the water saturation time is 8 hours. The anaerobic water is based on completely submerging the raw materials; Thawing treatment, in which t...

Embodiment 3

[0023] A method for extracting eel small-molecule active peptides from eel bones, comprising the following steps: (1) eel bone treatment: clean fresh eel bones, crush them, and use a temperature of 125° C. in a cooking tank at a temperature of 0.14 Mpa pressure cooking 8h, bone dregs are discharged to obtain bone broth, after quick freezing, frozen bone broth oil jelly blocks are formed, stored in a freezer at -18°C for subsequent use; The frozen bone broth jelly block is crushed at a low temperature, and the crushing temperature is controlled at 2° C., and the frozen bone broth jelly block is pulverized into frozen bone broth jelly block particles with a particle diameter of 0.04 mm; (3) cell dissolution Treatment: use 4°C anaerobic water under normal pressure to carry out impregnation water treatment on the crushed frozen bone broth jelly block particles, the water saturation time is 7 hours, and the anaerobic water is subject to completely submerging the raw materials; then ...

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PUM

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Abstract

The invention relates to a method for extracting sea eel small molecular active peptide from sea eel bones. The method comprises the following steps of 1, sea eel bone processing, 2, low-temperature smashing, 3, cell dissolution processing, 4, biological enzymolysis processing and 5 postprocessing; the ratio of the obtained sea eel small molecular peptide with the molecular weight being 0-480 d is 88.5% or more; the sea eel small molecular peptide is applicable to a peptide for diagnosis, a peptide vaccine, an antibacterial activity peptide, a cell factor simulation peptide, a cell factor simulation peptide, an antiviral peptide or an anti-tumor peptide and a peptide health care product.

Description

technical field [0001] The invention relates to a technique for extracting peptides from fish bones, in particular to a method for extracting small molecule peptides from eel bones. Background technique [0002] Collagen extracted from animals such as yak bones has certain limitations in absorption rate, and with the risk of mad cow disease, this more traditional method is not recognized by people, and people increasingly recognize it from The corresponding nutrients are extracted from fish. [0003] The current extraction is mainly to extract collagen from fish scales and fish skins, such as tilapia fish scales, deep-sea fish skins such as cod skins, but these extracts have a wide molecular weight distribution and are not easy to absorb. The existence of outstanding problems such as low absorption rate has greatly prevented the realization of new health care concepts such as high nutritional value, safety, and good absorption that modern people pursue. [0004] On the oth...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 潘爱国
Owner 潘爱国
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